Front. Vet. Sci. Frontiers in Veterinary Science Front. Vet. Sci. 2297-1769 Frontiers Media S.A. 10.3389/fvets.2025.1749139 Correction Correction: Isolation, identification of porcine a rotavirus, and preparation of the monoclonal antibodies Hu Yu 1 2 Wu Hongjin 1 2 Zha Boqian 1 2 Song Deping 1 2 Wu Xiangdong 1 2 Wu Huansheng 1 2 * Department of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China Correspondence: Huansheng Wu, huanswu@jxau.edu.cn 18 12 2025 2025 12 1749139 18 11 2025 20 11 2025 24 11 2025 Copyright © 2025 Hu, Wu, Zha, Song, Wu and Wu. 2025 Hu, Wu, Zha, Song, Wu and Wu https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. A Correction on Isolation, identification of porcine a rotavirus, and preparation of the monoclonal antibodies by Hu, Y., Wu, H., Zha, B., Song, D., Wu, X., and Wu, H. (2025). Front. Vet. Sci. 12:1689520. doi: 10.3389/fvets.2025.1689520 neonatal piglet diarrhea rotavirus isolation and identification monoclonal antibodies indirect ELISA method section-at-acceptance Veterinary Infectious Diseases

There was a mistake in Figure 2G as published.

The IFA image intended to show “the identification of fluorescent response in MA104 cells infected with G5 porcine rotavirus using mAb 1D3” was unintentionally misplaced from another experiment. This was solely due to a mislabeling of image files during figure assembly and does not affect the experimental results, underlying data, or the scientific conclusions of our study in any way. It was an inadvertent presentational oversight. The corrected figure appears below.

Preparation of monoclonal antibodies against rotavirus VP6 protein. This figure illustrates the construction of the pET30a-VP6 prokaryotic expression vector, protein purification, animal immunization, serum antibody detection, and the application of mAbs 1C7/1D3 in indirect immunofluorescence assays, with all experiments conducted in three independent replicates to ensure statistical robustness. (A) Colony PCR Verification of the pET-30a-VP6 Recombinant Plasmid. (B) Induced Expression and Protein Purification of the Recombinant Strain pET-30a-VP6-BL21(DE3)Plyss. (C) Recombinant VP6 protein was identified using an anti-His monoclonal antibody. (D) Recombinant VP6 protein was verified with PoRV-positive serum. (E) Serum antibody titers were determined via indirect enzyme-linked immunosorbent antibody assay (ELISA). (F) Isotype and Subclass Characterization of mAbs 1C7/1D3. (G) Fluorescent Reactions of mAbs 1C7/1D3 to Three Genotypes of PoRV (100 μm).

Gel electrophoresis (A), protein expression analysis with SDS-PAGE (B), Western blot showing His-tagged VP6 protein (C), reactivity with mouse serum (D), ELISA antibody titration graph with immunized mice (E), bar chart showing OD450 readings for mAb 1C7 and 1D3 (F), and fluorescent microscopy images showing cell staining with different antibodies (G).

Alt-text for Figure 2 has been modified to align with this correction.

The original version of this article has been updated.

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Edited and reviewed by: Kaiqing Zhang, Biocytogen, United States