AUTHOR=Zhang Shuxin , Cang Ming , Alatan Suhe , Gan Zhuer , Naren Zhana , Bu He , Cao Guifang , Tong Bin 
  
TITLE=Polymorphisms in the 3′UTR of the TGF-β1 gene associated with litter size in Ujimqin and Sonid sheep
  
JOURNAL=Frontiers in Veterinary Science
  
VOLUME=Volume 12 - 2025
  
YEAR=2026
  
URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1700201
  
DOI=10.3389/fvets.2025.1700201
  
ISSN=2297-1769
  
ABSTRACT=Reproductive characteristics like ovulation frequency and litter number significantly impact the sheep industry. Transforming growth factor beta 1 (TGF-β1) functions as a multifunctional regulator of key reproductive processes, plays a pivotal role in mammalian ovarian development. However, research on the role of the TGF-β1 gene in the reproduction of Ujimqin and Sonid sheep breeds remains scarce. Thus, this study identified 11 new variants of the TGF-β1 gene in these breeds by direct sequencing. In these 11 variants, the Sonid-linkage disequilibrium (LD)1 and Ujimqin-LD1 (comprising the g.50044287 G > A and g.50044526 G > C polymorphisms) in the 3′ untranslated region of the TGF-β1 gene were significantly associated with litter size in Sonid and Ujimqin ewes (p < 0.01, p < 0.05, respectively), and the single g.50044837 C > T polymorphism in the 3′UTR was also associated with litter size in Sonid sheep (p < 0.01) through association analyses. These findings may provide potential genetic markers for improving prolificacy in sheep.