Front. Vet. Sci. Frontiers in Veterinary Science Front. Vet. Sci. 2297-1769 Frontiers Media S.A. 10.3389/fvets.2022.861137 Veterinary Science Original Research Proteomic Investigation Reveals Eukaryotic Translation Initiation Factor 5A Involvement in Porcine Reproductive and Respiratory Syndrome Virus Infection in vitro Li Huawei 1 Wan Bo 2 Jiang Dawei 2 Ji Pengchao 2 Zhao Mengmeng 3 Li Xinfeng 1 Li Rui 4 * Qiao Songlin 4 * 1Henan Key Laboratory of Innovation and Utilization of Unconventional Feed Resources, Henan University of Animal Husbandry and Economy, Zhengzhou, China 2College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China 3School of Life Science and Engineering, Foshan University, Foshan, China 4Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China

Edited by: Yulong Gao, Harbin Veterinary Research Institute (CAAS), China

Reviewed by: Dang Wang, Huazhong Agricultural University, China; Kun Zhang, Virginia Commonwealth University, United States

 *Correspondence: Rui Li lirui860620@sina.com Songlin Qiao cdj565@gmail.com

This article was submitted to Veterinary Infectious Diseases, a section of the journal Frontiers in Veterinary Science

 13 04 2022 2022 9 861137 24 01 2022 18 03 2022 Copyright © 2022 Li, Wan, Jiang, Ji, Zhao, Li, Li and Qiao. 2022 Li, Wan, Jiang, Ji, Zhao, Li, Li and Qiao

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Porcine reproductive and respiratory syndrome virus (PRRSV), one of the most serious animal pathogens in the world, has caused enormous global swine industry losses. An in-depth investigation of the PRRSV-host interaction would be beneficial for preventing and controlling PRRSV infections and transmission. In this study, we performed label-free quantitative proteomic assays to investigate proteome dynamics of porcine alveolar macrophages (PAMs) during infection with highly pathogenic PRRSV (HP-PRRSV) strain HN07-1. Analysis of the results led to identification of 269 significantly differentially expressed host cellular proteins, of which levels of proteins belonging to the eukaryotic translation initiation factor (eIF) family were found to be decreased in abundance in HP-PRRSV-infected PAMs. Furthermore, knockdown of eIF5A expression was demonstrated to markedly suppress HP-PRRSV propagation, as reflected by reduced progeny virus titers in vitro. These results highlight the importance of eIF5A in PRRSV infection, while also demonstrating that PAMs down-regulate eIF5A expression as a host cell antiviral strategy. Results of the current study deepen our understanding of PRRSV pathogenesis and provide novel insights to guide development of effective strategies to combat the virus.

 proteome eIF5A PRRSV infection PAMs 31902284 31902279 National Natural Science Foundation of China10.13039/501100001809 Introduction

Porcine reproductive and respiratory syndrome (PRRS), a highly contagious disease caused by PRRS virus (PRRSV) infection, leads to reproductive disorders in sows and respiratory symptoms in pigs of all ages (1). PRRS was first discovered in North America in 1987 before it spread around the world, to cause substantial economic swine industry losses (2). In 2006, a severe epidemic of highly pathogenic (HP)-PRRS occurred in China that induced high fever in pigs and was associated with high mortality regardless of age. Importantly, the viral strain that was responsible for the epidemic possessed a 30-amino-acid discontinuous deletion within PRRSV non-structural protein 2 (nsp2) (3, 4).

PRRSV belongs to the family Arteriviridae (genus Betaarterivirus) within the taxonomic order Nidovirales and possesses a single-stranded positive-sense RNA genome of 15.4 kb (5). All PRRSV isolates are classified into two genotypes: PRRSV-1 and PRRSV-2 (6). PRRSV-2 strains were predominant in China. The cell tropism of PRRSV, which is highly limited, includes porcine alveolar macrophages (PAMs) that act as primary host cells to support viral infection (7). Importantly, PRRSV infection is a complex process such that mechanisms associated with PRRSV infection have not yet been fully clarified in spite of intensive research efforts.

Quantitative proteomic techniques, which are classified as label-based and label-free methods, have been used to research viral infections such as influenza virus (8), human respiratory syncytial virus (9), hepatitis B virus (10), porcine circovirus type 2 (11), foot and mouth disease virus (12), infectious bursal disease virus (13), African swine fever virus (14, 15). Consequently, results of these studies have created a foundation of knowledge on which to build future investigations to better understand pathogenesis of other viruses, including PRRSV.

In recent years, researchers have studied PRRSV infection using quantitative proteomic approaches. For example, Fang et al. (16) used an acetylation-based antibody enrichment technique and a tandem mass tag label high-affinity purification liquid chromatography-mass spectrometry (LC-MS/MS) method to study acetylome regulation of antiviral activities in PRRSV-infected PAMs. A few years earlier, Zhang et al. (17) had generated a broad-spectrum ubiquitination modification map of PRRSV-infected PAMs using ubiquitination antibody enrichment in combination with MS technology. Slightly earlier, Li et al. (18) conducted label-free quantitative proteomics to detect differentially secreted proteins in supernatants of PRRSV-infected PAMs and compared the results to those obtained for supernatants of uninfected PAMs. These studies not only enhanced our understanding of PRRSV infection, but also identified potential targets of antiviral drugs.

In order to better understand the proteome alterations of host cells during PRRSV infection, a label-free quantitative approach coupled with LC-MS/MS was applied to analyze the altered proteins in HP-PRRSV HN07-1-infected PAMs. The results of these experiments revealed that at 24 h post-infection (hpi), expression levels of 269 host cellular proteins were found to be significantly altered. Subsequent bioinformatic analyses revealed that these differentially expressed proteins were enriched for functional terms corresponding to several biological processes and KEGG pathways. Interestingly, expression levels of translation-related proteins, including eukaryotic translation initiation factors (eIFs), were significantly down-regulated after HP-PRRSV HN07-1 infection, with one such factor, eIF5A, found to be essential for viral replication. The results of this work uncovered for the first time the importance of eIF5A during virus infection, while also providing insights into host-pathogen interactions that occur during PRRSV infection to guide future development of effective antiviral strategies.

 Materials and Methods Cells and Virus

PAMs were collected from 4-week-old pathogen-free piglets that had been previously confirmed to be uninfected with PRRSV, porcine circovirus type 2, foot-and-mouth disease virus, pseudorabies virus, classical swine fever virus, and porcine parvovirus. The PAMs collection procedure was approved by the Ethical and Animal Welfare Committee of the Key Laboratory of Animal Immunology of the Ministry of Agriculture of China (permit no. 2018005). Collected PAMs were cultured in RPMI-1640 medium (Hyclone, China) containing 10% fetal bovine serum (FBS, Gibco, USA), 100 μg/mL streptomycin, and 100 units/mL penicillin (Hyclone, China) in a humidified incubator maintained at 37°C with 5% CO2. MARC-145 cells were purchased from ATCC and passaged in our laboratory. CRL-2843-CD163 cells were obtained from Professor Enmin Zhou of the College of Veterinary Medicine, Northwest Agriculture and Forestry University. The HP-PRRSV HN07-1 strain (GenBank: KX766378.1) that was used in this study was isolated and identified by the Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences (19).

 Virus Inoculation

PAMs were infected with HP-PRRSV HN07-1 at a multiplicity of infection (MOI) of 0.1 then the cells were fixed in ice-cold 95% methyl alcohol at 12, 24, 36, and 48 hpi. Next, cells were blocked in 5% skim milk in phosphate buffer solution with Tween-20 (PBST) at 4°C for 12 h. Thereafter, an immunofluorescence assay (IFA) was conducted to detect viral propagation at different time points post-infection using anti-PRRSV nucleocapsid (N) protein antibody as a probe (GeneTex, USA). Thereafter, cells were incubated with fluorescein isothiocyanate (FITC)-labeled goat-anti-mouse IgG (Sigma, USA) as secondary antibody. After incubation with primary and secondary antibodies, the cells were washed with PBST then 4′,6-diamidino-2-phenylindole-dihydrochloride (DAPI, Beyotime, China) was added to cells followed by incubation at room temperature for 30 min to stain the nuclei. Next, an OLYMPUS IX 81 confocal microscope equipped with a digital camera was used to capture the fluorescent images. Then the one-step growth curve of HP-PRRSV HN07-1 in PAMs was plotted based on virus titers obtained at 12, 24, 36, 48, 60, and 72 hpi.

 Sample Preparation, Protein Isolation, and Protein Digestion

PRRSV-infected and uninfected PAMs were gently washed with PBS, then were treated with 0.25% trypsin-EDTA (Solarbio, China) for 2 min. Next, cell suspensions were centrifuged at 1,200 rpm for 6 min then the cells were stored at −80°C until needed for further investigations. Protein extractions from PAMs were conducted as follows: frozen protein pellets were homogenized in lysis buffer (8 M urea, 2 M thiourea, 4% CHAPS, 20 mM Tris base, 30 mM dithiothreitol (DTT), and 2% Bio-Lyte) on ice. After that, samples were subjected to ultrasonic treatment followed by centrifugation at 13,500 rpm at 4°C for 20 min. Thereafter, each supernatant was removed then precipitated with ice-cold acetone at −20°C for 30 min then centrifuged twice (13,500 rpm for 10 min at 4°C) to pellet the proteins. Next, each pellet was collected and dissolved in 40 mM NH4HCO3 in which DTT was added to a final concentration of 100 mM, and iodoacetamide was also added to a final concentration of 50 mM. Thereafter, reductive alkylation was allowed to proceed for 1 h in dark at room temperature. Protein concentrations were quantified using the Bradford assay (20).

To conduct protein digestions, denatured proteins were reduced in 100 mM DTT and alkylated with 50 mM iodoacetamide to prevent reformation of disulfide bonds. Next, samples were digested with sequencing grade modified trypsin (Promega, USA) then were incubated at 37°C for 14 h. Finally, peptides were pooled and dried using a Speed-Vac system (RVC 2–18, Marin Christ, Germany) then were analyzed via MS/MS.

 LC–MS/MS Analysis

Each dried sample was dissolved in 1 formic acid aqueous solution and centrifuged at 14,000 rpm for 20 min at 4°C. Next, each supernatant was gently transferred to a clean tube to avoid creation of bubbles. Prior to MS analysis, digested peptides were suspended in 15 μL of 0.1% formic acid then 10 μL of each peptide sample was subjected to LC-MS/MS analysis using an Easy nLC1000 System (Thermo Fisher Scientific, USA) coupled to a Q Exactive Orbitrap Spectrometer (Thermo Fisher Scientific) that was equipped with a nanoelectrospray ion source (capillary temperature 275°C, spray voltage 2.3 kV, and S-Lens RF 55%). After addition of loading solvent (2% acetonitrile and 0.1% formic acid in H2O) to the tryptic digests, the samples were loaded onto an Easy-Spray column filled with 2 μm C18 resin (75 μm × 50 cm, 100 Å, Thermo Fisher Scientific). Peptides were separated over a period of 130 min using a gradient consisting of 3 to 30% acetonitrile (containing 0.1% formic acid) using an analytical column packed with 3 μm C18 (75 μm × 15 cm, 100 Å, Thermo Fisher Scientific). The mass spectrometer was tuned to positive ion mode, MS scan control was maintained using Xcalibur software 2.2 (Thermo Fisher Scientific), MS data acquisition was data-dependent, repetition count was set to 1, exclusion duration was set to 30 s, and dynamic exclusion was enabled. MS1 precursor scan (m/z 300–2,000) acquisition was carried out in the orbitrap with a nominal resolution of 30,000 at m/z 400. Next, MS/MS fragmentation of the top 20 most intense multiply charged precursor ions was conducted using higher energy collisional dissociation with 35% normalized fragmentation energy. MS2 scans (m/z 100–2,000) were performed using the orbitrap mass analyzer at a resolution setting of 15,000 at m/z 400 and a starting m/z setting of 100.

 Data Analysis and Protein Quantification

MS raw data files were retrieved using Xcalibur 2.2 (Thermo Fisher Scientific) and searched using PEAKS 7.5 against the Sus Scrofa database, which contained 38,431 entries when it was downloaded in July 2020 from the NCBI-ref database (21). Search parameters were set as follows: parent mass error tolerance of 20.0 ppm, fragment mass error tolerance of 0.05 Da, enzyme was trypsin. No specific cleavage site was selected for the peptide, maximum missed cleavages per peptide was set to 2, carbamidomethyl (C, +57.02) was selected as the fixed modification, and oxidation (M, +15.99) was selected as the variable modification. Each peptide had at most three types of posttranslational modifications. Protein selection parameters were as follows: false discovery rate (FDR) was ≤1.0% (-10l g P ≥ 20.0) and unique peptide with one spectrum was ≥1. The results of database retrieval were quantitatively analyzed using PEAKS Q and the peptide rate was calculated according to peak area. The conditions were set as follows: retention time shift tolerance of 1 min, mass error tolerance of 15 ppm, unique peptide ≥1, charge between 2 and 8, fold change of proteins and peptides was ≥1.5, and significance of ≥3 (P ≤ 0.05).

 Bioinformatics Analysis

All identified proteins were used as inputs for functional analysis using ClueGO V2.1.7, a Cytoscape plug-in (http://www.ici.upmc.fr/cluego/), that comprehensively identifies proteins that are involved in various biological signal pathways and protein interactions (22). Protein FASTA files were blasted against the Sus scrofa database using GI numbers. Right-sided hypergeometric enrichment was conducted as a statistical test using the Bonferroni step-down correction method (P ≤ 0.05) using parameters of gene ontology score range of three to eight, Kappa threshold set to 0.4, and initial group size set to one. Protein-protein interaction (PPI) networks were constructed using another Cytoscape plug-in, GeneMANIA (23), which uses many functional association data, including protein and genetic interactions.

 Real-Time PCR (RT-PCR)

Samples of total RNA of mock- and PRRSV-infected PAMs were prepared using TRIzol reagent (Invitrogen, USA) at 12 and 24 hpi, then cDNAs were generated from total RNA preparations using a reverse transcriptase kit according to the manufacturer's instructions (Takara, Japan). β-Actin served as an internal reference to normalize the data. Primer sequences are listed in Supplementary Table 1. RT-PCR assays were conducted using an Applied Biosystems 7,500 Fast RT-PCR System with 20 μL reactions (performed in triplicate) prepared that contained SYBR Green Premix 10 μL (ROCHE, Switzerland), 0.6 μL of each primer, and 6.8 μL of H2O. Each experiment was performed independently three times.

 UV-Inactivation of PRRSV

The virus solution was irradiated by exposure to ultraviolet light of wavelength 254 nm that was emitted by a low-intensity ultraviolet lamp (120 mJ/cm2). Irradiation was conducted at room temperature for 30 min to inactivate the virus; the effectiveness of inactivation was assessed using RT-PCR.

 Western Blot (WB)

Mock-infected, PRRSV-infected, and UV-inactivated PRRSV-treated PAMs were harvested at 0, 6, 12, and 24 hpi then the cells were lysed in RIPA buffer (Solarbio) containing 1% PMSF (Solarbio) for 30 min on ice followed by measurement of protein concentrations. For WB analysis, cell lysates containing equivalent concentrations of total protein were subjected to 12% SDS-PAGE then the separated proteins were transferred to 0.45 μm polyvinylidene difluoride (PVDF) membranes (Millipore, USA). After membranes were blocked in 5% skim milk at 4°C overnight, membranes were incubated with polyclonal antibodies specific for myxovirus-resistant protein 1 (Mx1) (Proteintech, China), tetratricopeptide repeats 3 (IFIT3) (Proteintech), PRRSV N (GeneTex, USA), or monoclonal antibody (mAb) specific for signal transducer and activator of transcription 1 (STAT1), eIF5A, eIF4E, eIF4H, 4EBP1, β-Actin (Cell Signaling Technology, USA). After being washed with PBST for three times, the membranes were treated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG or goat anti-mouse IgG secondary antibody (Abbkine, USA). A chemiluminescence kit (Beyotime) was used to detect signals resulting from antibodies binding to membrane-bound proteins.

 SiRNA Transfection

SiRNAs targeting eIF5A, eIF4E and the negative control (NC) were synthesized by Gene Pharma (Shanghai, China) as described in Supplementary Table 2. CRL-2843-CD163 cells or PAMs were transfected with each indicated siRNA at a final concentration of 0.2 mM using Lipofectamine RNAiMAX (Invitrogen, USA) according to the manufacturer's instructions. Effects of transfected siRNAs after 24, 36, and 48 h were verified by RT-PCR using primers that are listed in Supplementary Table 1 and by WB analysis based on binding of mAb probes to eIF5A and eIF4E proteins. The rescue experiment was carried out according to a previous study (24), the siRNA targeting the 3′untranslated region (UTR) of eIF5A which was used in rescue experiment was synthesized by Gene Pharma (sequence is listed in Supplementary Table 2) and was used in the eIF5A knockdown experiment conducted in CRL-2843-CD163 cells. Cytotoxicity was assessed at 24, 36, and 48 h after siRNA transfection by adding MTS reagent to cells followed by incubation of cells at 37°C for 1 h. Absorbance was measured at 490 nm.

 Effect of <italic>SiRNA-eIF5A</italic> on PRRSV Infection

After CRL-2843-CD163 cells or PAMs were subjected to eIF4E and eIF5A knockdown with appropriate siRNAs (or NC control), the cells were inoculated with HP-PRRSV HN07-1 (MOI = 0.1) and harvested at 12 and 24 hpi for RT-PCR analysis. CRL-2843-CD163 cells or PAMs after eIF5A knockdown were inoculated with HP-PRRSV HN07-1 (MOI = 0.1) and harvested at 24 hpi for IFA and WB analyses. PAMs after eIF5A knockdown were infected with HP-PRRSV HN07-1 (MOI = 0.1) and harvested at 48 hpi for determination of the median tissue culture infective dose (TCID50), which was conducted as follows: MARC-145 cells were cultured in 96-well plates overnight in DMEM (Solarbio) containing 10% FBS. Next, the cells were inoculated with diluted PRRSV at a MOI of 0.1 followed by incubation at 37°C for 3 h. After the cells were washed, DMEM containing 2% FBS was added to each well-then viral yields were calculated based on TCID50 values determined at 48 hpi as per the Reed-Munch method (25).

 EIF5A Rescue Experiments

To determine whether recombinant eIF5A expression could reverse suppression of PRRSV infection due to eIF5A knockdown, we cloned eIF5A into a eukaryotic expression vector. Briefly, cDNA encoding eIF5A was amplified via PCR from full-length eIF5A cDNA (Gene ID: 100517970) using the primers listed in Supplementary Table 1. Next, the amplified PCR product was isolated and inserted into the 3*Flag-CMV-7.1 eukaryotic expression vector (Sigma) between the HindIII and XbaI sites. The recombinant 3*Flag-CMV-eIF5A eukaryotic expression vector was validated by the Shanghai Sangon Biotech Co. Ltd. (Shanghai, China). WB was used to confirm expression of 3*Flag-CMV-eIF5A protein in cells using anti-eIF5A mAb and anti-Flag mAb (Sigma). After siRNA targeting the 3′UTR of eIF5A was transfected into CRL-2843-CD163 cells, recombinant 3*Flag-CMV-eIF5A was transfected into the same cells then PRRSV propagation in the CRL-2843-CD163 cells was evaluated by RT-PCR, IFA, WB, and TCID50 assay. Cytotoxicity of 3*Flag-CMV-eIF5A after it was transfected into CRL-2843-CD163 cells (after eIF5A knockdown) was assessed at 24, 36, and 48 h post-transfection using the same method as described above.

 Statistical Analysis

Experimental data were expressed as the mean and ± standard deviation (SD) based on triplicate samples then data were analyzed via Student's t-test using GraphPad Prism software (v8.0). Statistical significance is indicated in the figures as *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

 Results Propagation Kinetics of HP-PRRSV HN07-1 in PAMs

First, PAMs were infected with HP-PRRSV HN07-1 at a MOI of 0.1. Next, to determine the appropriate time point for proteomic analysis, IFA was conducted of infected cells using a mAb probe specific for the viral N protein in order to determine the kinetics of HP-PRRSV HN07-1 infection in PAMs. As shown in Figure 1A, specific immunofluorescence became visible as early as 12 hpi that indicated that PRRSV propagation began at that time, with fluorescence significantly increasing after 24 hpi. At 36 hpi, fluorescence reached its maximum value then decreased at 48 hpi (Figure 1A). Based on these results, the one-step growth curve for HP-PRRSV HN07-1 in PAMs was plotted and it revealed that viral titers of HP-PRRSV HN07-1 reached a maximum of approximately 107.2 TCID50/mL at 36 hpi then gradually declined (Figure 1B). Based on IFA and growth curve results, we chose the time point of 24 hpi (before the titer peaked at 36 hpi) for use in subsequent proteomic analyses.

The propagation kinetics of HP-PRRSV HN07-1 in PAMs. (A) PAMs were infected with HP-PRRSV HN07-1 at MOI = 0.1 for 12, 24, 36, and 48 h or were mock-infected for 36 h as a control. Fluorescent images were recorded using an OLYMPUS IX 81 confocal microscope based on detection of virus using anti-PRRSV N protein antibody. (B) The one-step growth curve of HP-PRRSV HN07-1 in PAMs based on viral titers at 12, 24, 36, 48, 60, and 72 hpi.

 LC-MS/MS Detection

Using mock-infected and HP-PRRSV HN07-1-infected PAMs, label-free quantitative proteome analysis was performed on triplicate samples then the data for each sample were analyzed using Peaks 7.5 software tool in order to conduct database searching and quantitative analysis. The results revealed detection of total numbers of peptides (29,854, 26,947) and proteins (3794, 3558) in mock-infected and HP-PRRSV HN07-1-infected PAMs, respectively (Supplementary Tables 3–6). All proteomic data were deposited into the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) iProX partner repository (26) using the dataset identifier PXD026209.

 Protein Quantification

Next, the quantitative function tool of PEAKS 7.5 (Bioinformatics Solutions Inc.) was used to examine clusters of significantly different proteins identified in HP-PRRSV HN07-1-infected PAMs at 24 hpi (>1.5-fold, P < 0.05). Ultimately, 269 significantly differentially expressed proteins were identified after HP-PRRSV HN07-1 infection (P < 0.05), of which 46 proteins were significantly up-regulated and 223 proteins were significantly down-regulated (Tables 1, 2). Importantly, for samples tested in triplicate, the heatmap indicated good repeatability (Figure 2). More information can be found in Supplementary Table 7.

The significantly up-regulated proteins in HP-PRRSV HN07-1-infected PAMs identified by LC-MS/MS.

No Accession Coverage (%) Peptides Unique Group Profile Description
(Ratio)
1 gi|346421333 17 2 1 00:01.0 Non-histone chromosomal protein HMG-17
2 gi|324123893 9 3 3 00:01.0 Interferon-induced protein with tetratricopeptide repeats 3
3 gi|346986269 8 3 3 00:01.0 Interferon-induced protein with tetratricopeptide repeats 1
4 gi|545867461 8 5 4 00:01.0 Interferon-induced GTP-binding protein Mx1
5 gi|148231384 31 1 1 00:01.0 Thymosin beta-10
6 gi|311258597 1 1 1 00:01.0 Rootletin
7 gi|311250758 21 1 1 00:01.0 Interferon-induced transmembrane protein 1
8 gi|545812163 11 1 1 00:01.0 Histidine triad nucleotide-binding protein 1-like
9 gi|350529421 8 1 1 00:01.0 Mitochondrial ribosomal protein L49
10 gi|545884074 57 6 5 1.00:31.22 Thymosin beta-4
11 gi|545892076 15 3 3 1.00:11.28 Interferon-induced 17 kDa protein
12 gi|350583970 2 1 1 1.00:4.87 Keratin type II cytoskeletal 75
13 gi|345441792 2 1 1 1.00:3.31 Alcohol dehydrogenase 1C (class I) gamma polypeptide
14 gi|47522622 4 1 1 1.00:3.05 Galectin-9
15 gi|312283580 14 4 4 1.00:2.94 Superoxide dismutase [Mn] mitochondrial
16 gi|194036463 22 4 1 1.00:2.77 Ras-related protein Rap-1A
17 gi|545855071 2 1 1 1.00:2.77 Von Willebrand factor A domain-containing protein 8
18 gi|356460981 1 1 1 1.00:2.64 Probable ATP-dependent RNA helicase DDX58
19 gi|148235632 31 9 8 1.00:2.63 Interferon-induced GTP-binding protein Mx2
20 gi|350580091 5 1 1 1.00:2.61 Proteoglycan 3
21 gi|545811106 5 2 2 1.00:2.35 Sequestosome-1
22 gi|350582355 2 1 1 1.00:2.33 Exportin-1
23 gi|350539043 3 1 1 1.00:2.18 Phospholipid scramblase 1
24 gi|545808740 5 1 1 1.00:2.12 60S ribosomal protein L18a
25 gi|350586371 1 1 1 1.00:2.11 Coagulation factor XIII A chain
26 gi|297632416 14 2 2 1.00:1.88 Enhancer of rudimentary homolog
27 gi|148233143 3 1 1 1.00:1.81 Prolyl 4-hydroxylase subunit alpha-1 precursor
28 gi|545859997 3 1 1 1.00:1.80 Beta-arrestin-2
29 gi|72535204 4 1 1 1.00:1.79 Nicotinamide phosphoribosyl transferase
30 gi|194036682 3 1 1 1.00:1.77 Gamma-glutamyl hydrolase
31 gi|47523066 4 1 1 1.00:1.77 Caspase-3
32 gi|545821746 3 1 1 1.00:1.74 Tyrosine-protein kinase Lyn
33 gi|545856413 1 2 2 1.00:1.70 E3 ubiquitin-protein ligase RNF213-like partial
34 gi|47522754 1 1 1 1.00:1.68 Trifunctional enzyme subunit alpha mitochondrial
35 gi|237681310 24 3 3 1.00:1.66 Protein S100-A8
36 gi|545880266 3 1 1 1.00:1.65 RNA-binding protein Raly
37 gi|194034801 17 1 1 1.00:1.63 Normal mucosa of esophagus-specific gene 1 protein
38 gi|47523306 4 3 3 1.00:1.61 Signal transducer and activator of transcription 1
39 gi|350539097 10 1 1 1.00:1.61 Ubiquitin/ISG15-conjugating enzyme E2 L6
40 gi|347300243 9 5 5 1.00:1.57 Glutamate dehydrogenase 1 mitochondrial
41 gi|148225750 3 2 2 1.00:1.57 Heat shock protein 105 kDa
42 gi|350589740 11 1 1 1.00:1.56 60S ribosomal protein L21
43 gi|350580983 17 3 3 1.00:1.53 Receptor expression-enhancing protein 5
44 gi|50979305 1 1 1 1.00:1.52 Sialoadhesin precursor
45 gi|335279372 6 1 1 1.00:1.51 Myristoylated alanine-rich C-kinase substrate
46 gi|545887382 6 1 1 1.00:1.51 B-cell receptor-associated protein 31

The significantly down-regulated proteins in HP-PRRSV HN07-1-infected PAMs identified by LC-MS/MS.

No Accession Coverage (%) Peptides Unique Group profile Description
(Ratio)
1 gi|264681454 8 2 2 1.00:0.67 S-adenosylmethionine synthase isoform type-2
2 gi|545801818 15 6 6 1.00:0.66 Cytosolic non-specific dipeptidase
3 gi|47522870 3 1 1 1.00:0.66 Serine/threonine-protein phosphatase 2A 65kDa regulatory subunit
4 gi|47522828 2 1 1 1.00:0.66 Transferrin receptor protein 1
5 gi|113205886 7 1 1 1.00:0.66 Nucleoside diphosphate kinase B
6 gi|350581449 9 3 1 1.00:0.66 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
7 gi|335282345 12 1 1 1.00:0.66 Mitochondrial import inner membrane translocase subunit Tim13
8 gi|347300400 6 1 1 1.00:0.66 Core histone macro-H2A.1
9 gi|311254887 3 1 1 1.00:0.66 Gasdermin-D
10 gi|346716314 18 2 2 1.00:0.65 Rho GDP dissociation inhibitor (GDI) beta
11 gi|545810927 5 2 2 1.00:0.65 Polypyrimidine tract-binding protein 1
12 gi|335283403 10 5 4 1.00:0.65 Lamin-B1
13 gi|350596594 3 1 1 1.00:0.65 Malate dehydrogenase cytoplasmic-like
14 gi|311258550 19 5 4 1.00:0.64 EF-hand domain-containing protein D2
15 gi|346644866 5 1 1 1.00:0.64 Coiled-coil-helix-coiled domain-containingprotein 3 mitochondrial
16 gi|148230268 25 8 8 1.00:0.64 Galectin-3
17 gi|545804280 6 1 1 1.00:0.64 Erythrocyte band 7 integral membrane protein
18 gi|350594565 13 5 5 1.00:0.64 Acid ceramidase-like
19 gi|545894790 28 4 4 1.00:0.64 Ribonuclease inhibitor partial
20 gi|545815108 2 1 1 1.00:0.64 Coronin-7
21 gi|311268187 7 1 1 1.00:0.64 Thioredoxin domain-containing protein 17 isoform 1
22 gi|545884463 7 4 4 1.00:0.64 Cytochrome b-245 heavy chain
23 gi|47522784 8 2 2 1.00:0.64 Fructose-1 6-bisphosphatase 1
24 gi|311252239 2 1 1 1.00:0.64 All-trans-retinol 13,14-reductase
25 gi|545877341 6 1 1 1.00:0.64 NAD(P) transhydrogenase mitochondrial-like
26 gi|545806957 9 31 31 1.00:0.63 AHNAK nucleoprotein
27 gi|335296249 14 2 2 1.00:0.63 Cysteine and glycine-rich protein 1-like isoform 3
28 gi|545894785 3 1 1 1.00:0.63 Heterogeneous nuclear ribonucleoprotein H2
29 gi|335296435 9 5 5 1.00:0.63 Cytoplasmic aconitate hydratase
30 gi|178056616 12 2 2 1.00:0.63 Rho-related GTP-binding protein RhoG
31 gi|545893961 5 1 1 1.00:0.63 Mannose-P-dolichol utilization defect 1 protein-like
32 gi|545894799 13 1 1 1.00:0.63 Nuclear transport factor 2-like partial
33 gi|172072665 5 4 2 1.00:0.63 Hexokinase-2
34 gi|343790912 22 4 2 1.00:0.63 Ras-related C3 botulinum toxin substrate 1
35 gi|350593002 10 1 1 1.00:0.63 Sideroflexin-3
36 gi|194043861 21 10 1 1.00:0.63 Tubulin alpha-1D chain
37 gi|229892818 21 2 2 1.00:0.62 Prothymosin alpha
38 gi|350587143 9 3 3 1.00:0.62 Legumain
39 gi|347300396 11 3 3 1.00:0.62 Histamine N-methyltransferase
40 gi|165973416 11 3 3 1.00:0.62 SLA class II histocompatibility antigen DQ haplotype C beta chain precursor
41 gi|545870539 1 1 1 1.00:0.62 Vinculin
42 gi|545830766 4 1 1 1.00:0.62 Heterogeneous nuclear ribonucleoprotein L
43 gi|298104128 22 1 1 1.00:0.62 Reactive oxygen species modulator
44 gi|545819344 5 1 1 1.00:0.61 40S ribosomal protein S7-like
45 gi|545867661 16 11 11 1.00:0.61 Integrin beta-2
46 gi|347300387 13 2 2 1.00:0.61 60S ribosomal protein L17
47 gi|194044822 7 2 1 1.00:0.61 Peroxiredoxin-4
48 gi|311259613 12 2 2 1.00:0.61 40S ribosomal proteinS5 isoform 1
49 gi|328550534 4 1 1 1.00:0.61 Electron transfer flavoprotein subunit beta
50 gi|311267953 21 3 3 1.00:0.61 60S ribosomal protein L23a-like
51 gi|346227212 4 2 2 1.00:0.61 ribosomal protein L3
52 gi|335292095 2 1 1 1.00:0.61 Mitogen-activated protein kinase 14
53 gi|545854442 12 2 2 1.00:0.60 High mobility group protein B1
54 gi|51592135 20 3 3 1.00:0.60 Cofilin-1
55 gi|114326183 8 1 1 1.00:0.60 ADP-ribosylation factor 4
56 gi|545848409 4 1 1 1.00:0.60 Acetyl-CoA acetyltransferase mitochondrial
57 gi|545894465 19 4 4 1.00:0.59 14-3-3 protein epsilon-like
58 gi|545887655 6 2 2 1.00:0.59 Glucose-6-phosphate 1-dehydrogenase partial
59 gi|545834779 2 1 1 1.00:0.59 Tyrosine-tRNA ligase cytoplasmic
60 gi|545876142 2 1 1 1.00:0.58 Leucine-rich repeat flightless-interacting protein 2
61 gi|335278864 6 2 1 1.00:0.58 L-lactate dehydrogenase A-like 6B-like
62 gi|335287489 2 1 1 1.00:0.58 Tubulin tyrosine ligase-like family member 12
63 gi|113205762 2 1 1 1.00:0.58 Granulins precursor
64 gi|311277265 3 1 1 1.00:0.58 55 kDa erythrocyte membrane protein
65 gi|194034199 4 1 1 1.00:0.58 Proteasome subunit alpha type-3
66 gi|545812127 12 1 1 1.00:0.58 Alpha-aminoadipic semialdehyde dehydrogenase
67 gi|545876461 1 1 1 1.00:0.58 Low quality protein: vigilin
68 gi|113205616 24 4 4 1.00:0.57 60S ribosomal protein L10
69 gi|545895140 24 2 2 1.00:0.57 Filamin-A partial
70 gi|47523694 7 2 2 1.00:0.57 calpain small subunit 1
71 gi|311275455 10 1 1 1.00:0.57 V-type proton ATPase subunit F
72 gi|545869404 3 1 1 1.00:0.57 RNA-binding protein EWS
73 gi|47523866 4 1 1 1.00:0.57 Aldose 1-epimerase
74 gi|545883733 3 1 1 1.00:0.56 Transcriptional activator protein Pur-beta-like partial
75 gi|359465556 1 1 1 1.00:0.56 Active breakpoint cluster region-related protein
76 gi|47522760 2 1 1 1.00:0.56 Long-chain 3-ketoacyl-CoA thiolase
77 gi|347658980 10 1 1 1.00:0.55 Ribosomal protein L36
78 gi|113205704 7 1 1 1.00:0.55 Proteasome subunit beta type-10
79 gi|346421419 2 1 1 1.00:0.55 Lysosome membrane protein 2 precursor
80 gi|48675931 16 2 1 1.00:0.55 40S ribosomal protein S17
81 gi|335281954 2 1 1 1.00:0.55 Rho GTPase-activating protein 1
82 gi|545830671 2 1 1 1.00:0.55 26S proteasome non-ATPase regulatory subunit 8
83 gi|346986249 6 1 1 1.00:0.55 Proteasome subunit beta type-1
84 gi|545873280 10 1 1 1.00:0.54 Activated RNA polymerase II transcriptional coactivator p15-like
85 gi|545839827 2 2 2 1.00:0.54 Exportin-5
86 gi|523580068 1 1 1 1.00:0.54 Chaperonin containing TCP1 subunit 5 (epsilon)
87 gi|545894159 3 1 1 1.00:0.54 Beta-galactosidase-like
88 gi|545847157 2 1 1 1.00:0.54 Integrin-linked protein kinase
89 gi|545871130 5 2 2 1.00:0.53 Lysosomal acid lipase/cholesteryl ester hydrolase
90 gi|349501107 36 2 1 1.00:0.53 Ribosomal protein large P2
91 gi|311275636 5 2 2 1.00:0.53 Septin-7
92 gi|311254975 3 1 1 1.00:0.53 NADH-cytochrome b5 reductase 3-like
93 gi|545858849 4 2 2 1.00:0.53 Retinoid-inducible serine carboxypeptidase
94 gi|346421386 18 1 1 1.00:0.53 ATP synthase subunit g mitochondrial
95 gi|113205690 5 2 1 1.00:0.53 Protein phosphatase 1 catalytic subunit alpha isoform
96 gi|311261988 14 6 6 1.00:0.52 Phosphoglucomutase-2
97 gi|350584895 3 1 1 1.00:0.52 Protein VAC14 homolog partial
98 gi|545894912 6 2 2 1.00:0.52 Apolipoprotein B receptor partial
99 gi|545860680 2 1 1 1.00:0.52 Transient receptor potential cation channel subfamily V member 2
100 gi|350582454 1 1 1 1.00:0.52 Leucine-rich PPR motif-containing protein mitochondrial
101 gi|346986437 9 2 2 1.00:0.52 Family with sequence similarity 49 member B
102 gi|356582297 15 3 1 1.00:0.52 ADP-ribosylation factor-like 8B
103 gi|545828227 12 5 5 1.00:0.51 Leukotriene A-4 hydrolase
104 gi|47523292 15 2 2 1.00:0.51 CD74 antigen
105 gi|350595800 9 1 1 1.00:0.51 SH3 domain-binding glutamic acid-rich-like protein-like
106 gi|340007404 13 8 7 1.00:0.51 Alpha-actinin-1
107 gi|194040450 9 2 2 1.00:0.51 Lactoylglutathione lyase isoform 1
108 gi|545883591 7 1 1 1.00:0.51 Chromobox protein homolog 3
109 gi|335282824 3 1 1 1.00:0.51 IlvB (bacterial acetolactate synthase)-like
110 gi|335281298 14 6 1 1.00:0.50 Tubulin beta-4B chain
111 gi|324021713 20 6 6 1.00:0.50 Ribosomal protein S4
112 gi|545825344 10 1 1 1.00:0.50 UPF0160 protein MYG1 mitochondrial-like
113 gi|329663948 6 2 1 1.00:0.50 Ras GTPase-activating protein-binding protein 1
114 gi|47522648 2 1 1 1.00:0.50 Beta-hexosaminidase subunit beta precursor
115 gi|305855130 2 2 2 1.00:0.50 Valyl-tRNA synthetase
116 gi|178057125 4 1 1 1.00:0.50 Cathepsin Z precursor
117 gi|545825997 14 3 3 1.00:0.49 Proliferation-associated protein 2G4-like
118 gi|311255664 3 3 3 1.00:0.48 Extended synaptotagmin-1
119 gi|269914120 11 2 2 1.00:0.48 Lysozyme C-3 precursor
120 gi|545827028 6 2 2 1.00:0.48 Branched-chain-amino-acid aminotransferase cytosolic
121 gi|311274648 7 1 1 1.00:0.48 Sulfiredoxin-1-like
122 gi|348605266 7 1 1 1.00:0.48 Ribosomal protein S13
123 gi|55926217 32 3 3 1.00:0.47 Cytochrome c oxidase subunit 5B mitochondrial precursor
124 gi|545862394 8 2 1 1.00:0.47 Guanine nucleotide-binding protein G(i) subunit alpha-2
125 gi|335286747 12 1 1 1.00:0.47 Hepatoma-derived growth factor
126 gi|47522692 3 1 1 1.00:0.47 Long-chain specific acyl-CoA dehydrogenase mitochondrial precursor
127 gi|83921637 3 1 1 1.00:0.47 Matrix metalloproteinase-9
128 gi|223950631 42 2 2 1.00:0.46 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12
129 gi|47522940 3 1 1 1.00:0.46 Dihydrolipoyl dehydrogenase mitochondrial precursor
130 gi|345110604 4 1 1 1.00:0.46 LIM and senescent cell antigen-like domains 1
131 gi|335304552 8 3 3 1.00:0.45 Acetyl-coenzyme A synthetase 2-like mitochondrial
132 gi|545838059 4 1 1 1.00:0.45 BOLA class I histocompatibility antigen alpha chain BL3-7
133 gi|147899011 13 1 1 1.00:0.45 40S ribosomal protein S26
134 gi|335309772 8 1 1 1.00:0.45 Acyl-coenzyme A thioesterase 2 mitochondrial-like partial
135 gi|47522782 10 1 1 1.00:0.44 Beta-2-microglobulin precursor
136 gi|350593430 2 1 1 1.00:0.44 Glutathione reductase mitochondrial isoform 1
137 gi|545894793 11 1 1 1.00:0.44 Vesicle-associated membrane protein 3-like
138 gi|154147607 2 1 1 1.00:0.44 Calpain-2 catalytic subunit
139 gi|116175251 8 1 1 1.00:0.43 Macrophage migration inhibitory factor
140 gi|356460899 12 5 5 1.00:0.43 Catalase
141 gi|89886167 36 5 5 1.00:0.42 Fatty acid-binding protein epidermal NADH dehydrogenase
142 gi|148225172 15 1 1 1.00:0.42 [Ubiquinone] 1 alpha subcomplex subunit 4
143 gi|545803550 5 1 1 1.00:0.42 Stomatin-like protein 2 mitochondrial
144 gi|47523720 12 7 7 1.00:0.42 Glucose-6-phosphate isomerase
145 gi|47523764 52 7 2 1.00:0.40 Peptidyl-prolyl cis-trans isomerase A
146 gi|335289972 16 2 2 1.00:0.40 Neutral amino acid transporter B(0)-like
147 gi|311254226 13 1 1 1.00:0.40 40S ribosomal protein S27
148 gi|545859904 6 3 1 1.00:0.40 Beta-enolase
149 gi|47523126 3 1 1 1.00:0.40 Ficolin-2 precursor
150 gi|350597193 12 3 3 1.00:0.40 Peptidyl-prolyl cis-trans isomerase B
151 gi|545877091 2 1 1 1.00:0.40 Disabled homolog 2
152 gi|350584410 2 1 1 1.00:0.40 Lysophospholipid acyltransferase 5
153 gi|194035847 7 1 1 1.00:0.39 Astrocytic phosphoprotein PEA-15
154 gi|311262781 8 1 1 1.00:0.39 Ragulator complex protein LAMTOR3 isoform 1
155 gi|311247250 35 2 2 1.00:0.38 barrier-to-autointegration factor-like
156 gi|335300836 5 1 1 1.00:0.38 ES1 protein homolog mitochondrial
157 gi|545856802 5 1 1 1.00:0.38 CMRF35-like molecule 1
158 gi|47523668 8 1 1 1.00:0.38 Microsomal glutathione S-transferase 1
159 gi|350590733 1 2 2 1.00:0.37 Pre-mRNA-processing-splicing factor 8 partial
160 gi|363814526 1 1 1 1.00:0.37 Macrophage mannose receptor 1 precursor
161 gi|335287593 19 3 1 1.00:0.37 Ras-related C3 botulinum toxin substrate 2
162 gi|545844580 3 1 1 1.00:0.37 RNA-binding protein 47
163 gi|157279731 0 1 1 1.00:0.37 Myosin-1
164 gi|545831942 2 1 1 1.00:0.37 Glycogen [starch] synthase muscle
165 gi|47523548 24 2 2 1.00:0.36 Glutaredoxin-1
166 gi|311260951 3 1 1 1.00:0.36 Dehydrogenase/reductase SDR family member 1
167 gi|545875118 1 1 1 1.00:0.35 Splicing factor 3B subunit 1
168 gi|545858898 25 1 1 1.00:0.34 Dynein light chain 2 cytoplasmic-like
169 gi|350578528 13 2 2 1.00:0.34 Peptidyl-prolyl cis-trans isomerase B partial
170 gi|47522836 19 2 2 1.00:0.34 Osteoclast-stimulating factor 1
171 gi|545856859 14 1 1 1.00:0.34 60S ribosomal protein L38
172 gi|545881923 7 2 2 1.00:0.33 GTPase IMAP family member 4
173 gi|194018718 25 3 3 1.00:0.33 40S ribosomal protein S20
174 gi|307746897 15 2 2 1.00:0.33 Protein-L-isoaspartate(D-aspartate) O-methyltransferase
175 gi|311258112 3 1 1 1.00:0.33 Myeloid-associated differentiation marker
176 gi|47523692 41 5 5 1.00:0.32 Thioredoxin
177 gi|311270662 22 2 2 1.00:0.32 Phosphatidylethanolamine-binding protein 1
178 gi|47522916 4 1 1 1.00:0.32 Glutathione S-transferase omega-1
179 gi|545808462 10 2 2 1.00:0.30 ADP-ribosylation factor 1
180 gi|545855599 0 1 1 1.00:0.30 Dedicator of cytokinesis protein 9
181 gi|350582111 1 1 1 1.00:0.30 Eukaryotic translation initiation factor 5B
182 gi|343478222 9 1 1 1.00:0.29 Eukaryotic translation initiation factor 4H
183 gi|545804271 7 1 1 1.00:0.29 Ras-related protein Rab-14
184 gi|311245496 23 1 1 1.00:0.26 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit
185 gi|346227226 23 3 3 1.00:0.24 40S ribosomal protein S11
186 gi|55742824 4 1 1 1.00:0.24 Spliceosome RNA helicase DDX39B
187 gi|350582722 20 4 2 1.00:0.22 14-3-3 protein theta
188 gi|545803693 7 1 1 1.00:0.22 Clathrin light chain A
189 gi|545892437 11 2 2 1.00:0.21 CD9 antigen-like
190 gi|545817119 8 2 2 1.00:0.20 Pleckstrin
191 gi|317054710 4 1 1 1.00:0.20 Cytochrome c oxidase subunit II (mitochondrion)
192 gi|545834956 0 1 1 1.00:0.20 Microtubule-actin cross-linking factor 1
193 gi|194018698 21 2 2 1.00:0.20 Cytochrome c
194 gi|343478210 12 2 2 1.00:0.20 Syntaxin 12
195 gi|545894926 9 1 1 1.00:0.20 Vacuolar protein sorting-associated protein 35-like partial
196 gi|47523666 21 4 4 1.00:0.19 Proteasome activator complex subunit 1
197 gi|545839153 6 1 1 1.00:0.19 60S ribosomal protein L10a
198 gi|346644790 22 1 1 1.00:0.19 Eukaryotic translation initiation factor 4E-binding protein 1
199 gi|545870732 5 1 1 1.00:0.17 Uncharacterized protein
200 gi|545825732 5 1 1 1.00:0.17 CD63 antigen
201 gi|47523278 11 1 1 1.00:0.16 Resistin precursor
202 gi|194041813 3 1 1 1.00:0.16 Phosphatidylinositol 4-kinase type 2-alpha
203 gi|311250199 2 1 1 1.00:0.15 Heterogeneous nuclear ribonucleoprotein A0
204 gi|47522778 2 1 1 1.00:0.15 Scavenger receptor cysteine-rich type 1 protein M130 precursor
205 gi|237681312 43 4 4 1.00:0.14 Protein S100-A12
206 gi|545846943 6 1 1 1.00:0.13 Heterogeneous nuclear ribonucleoprotein D-like
207 gi|312233368 15 1 1 1.00:0.12 ATP synthase F0 subunit 8 (mitochondrion)
208 gi|311268292 19 3 3 1.00:0.10 Eukaryotic translation initiation factor 5A
209 gi|545803799 12 3 2 1.00:0.07 Acidic leucine-rich nuclear phosphoprotein 32 family member
210 gi|545806769 5 1 1 1.00:0.06 Reticulon-3
211 gi|48374063 6 3 1 1.00:0.06 Desmin
212 gi|147903958 21 1 1 1.00:0.04 Cystatin-B
213 gi|545851826 10 1 1 1.00:0.04 Acyl carrier protein mitochondrial
214 gi|47523802 8 1 1 1.00:0 Translationally-controlled tumor protein
215 gi|213021237 7 1 1 1.00:0 DNA-(apurinic or apyrimidinic site) lyase
216 gi|311252670 22 4 1 1.00:0 Calmodulin-like
217 gi|350579215 41 6 1 1.00:0 Peptidyl-prolyl cis-trans isomerase A-like
218 gi|545815969 0 1 1 1.00:0 E3 SUMO-protein ligase RanBP2
219 gi|48675935 6 1 1 1.00:0 60S ribosomal protein L32
220 gi|335281875 4 1 1 1.00:0 Proteoglycan 3-like
221 gi|342349338 1 1 1 1.00:0 ElaC homolog 2
222 gi|545894997 18 1 1 1.00:0 SH3 domain-binding glutamic acid-rich-like protein 3 partial
223 gi|47523608 5 1 1 1.00:0 Cytochrome b-245 light chain

Clustering analysis of significantly differentially expressed proteins in HP-PRRSV HN07-1-infected PAMs. Unsupervised hierarchical clustering of differentially expressed (fold change ≥2 and P < 0.05) proteins in HP-PRRSV HN07-1-infected PAMs. The columns represent mock cells and HP-PRRSV-HN07-1-infected PAMs for three replicate samples, while rows represent different proteins. Up-regulated and down-regulated proteins are indicated by red and green colors, respectively, with color intensity reflecting magnitudes of protein expression level changes, as shown in the legend at the upper right. More information can be found in Supplementary Table 7.

 Bioinformatics Analysis Based on ClueGo

ClueGo V2.1.7 was used to generate functionally grouped annotation networks so that we could functionally categorize significantly differentially expressed proteins associated with HP-PRRSV HN07-1 infection. As shown in Figure 3A, up-regulated proteins were mainly associated with functional terms such as response to interferon (IFN)-α (P = 1.03 × 10−5), IFN-β (P = 1.18 × 10−5), positive regulation of interleukin (IL)-8 production (P = 1.34 × 10−4), positive regulation of IL-1β production (P = 4.22 × 10−2), and regulation of Fc receptor-mediated stimulatory signaling pathway (P = 2.38 × 10−7). Down-regulated proteins were mainly associated with functional terms such as cytoplasmic translation (P = 1.78 × 10−04), translation (P = 1.02 × 10−11), translational initiation (P = 1.07 × 10−14), and antigen processing and presentation of exogenous peptide antigen (P = 2.8 × 10−06) (Figure 3B). Additional data are presented in Supplementary Tables 8, 9.

ClueGo was applied to enrich the biological process and KEGG pathways of significantly differentially expressed proteins identified in HP-PRRSV HN07-1-infected PAMs. (A) Biological process enrichment terms of significantly up-regulated proteins. (B) Biological process enrichment terms of significantly down-regulated proteins. (C) KEGG pathway enrichment terms of significantly up-regulated proteins. (D) KEGG pathway enrichment terms of significantly down-regulated proteins. The same color indicates similar functional categories. *P < 0.05, **P < 0.01. More information can be found in Supplementary Tables 8–11.

ClueGo V 2.1.7 was next used to conduct KEGG pathway analysis to explore potential functional networks of differentially expressed proteins. Up-regulated proteins were mainly enriched for KEGG pathway terms such as IL-6 signaling (P = 4.46 × 10−06), regulation of IFN signaling (P = 4.45757089317539 × 10−06), and termination of translesion DNA synthesis (P = 1.8771367 × 10−02) (Figure 3C). Down-regulated proteins were mainly enriched for the KEGG term ribosome (P = 7.5 × 10−08) (Figure 3D). Additional data are shown in Supplementary Tables 10, 11. Taken together, bioinformatic analysis results revealed that after HP-PRRSV HN07-1 infection, host innate immunity-related proteins and pathways were almost all up-regulated, while translation-related proteins and processes were dramatically down-regulated.

 PPI Network Analysis

To gain additional insights into possible functional interactions among the identified proteins, PPI networks were constructed using GeneMANIA (23) based on a large set of applicable association data pertaining to protein and genetic interactions (Figure 4). Due to the fact that the pig genome database is under-annotated, gene identifications for identified significantly differentially regulated proteins (listed in Tables 1, 2) were converted to human protein GI numbers. Next, predicted and integrated known PPI data sets from the Homo sapiens genome were input into GeneMANIA. Thereafter, GO categories were enriched for the input dataset using a false discovery rate (FDR)-corrected hypergeometric test.The results were compared to the background set of GO annotations for the entire Homo sapiens genome, with predicted, genetic, and physical interactions enabled during creation of the networks. The top 20 related genes and 20 attributes were displayed using GO biological process-based weighting, then Cytoscape was used to depict the networks. Figure 4A shows the interaction network for up-regulated proteins in HP-PRRSV HN07-1-infected PAMs that highlights interactions between host innate immunity and IFN I-related signaling pathway proteins. Figure 4B shows the interaction network for down-regulated proteins in HP-PRRSV HN07-1-infected PAMs that highlights the importance of interactions involving host proteins related to protein translation.

PPI network with significantly differentially expressed proteins in HP-PRRSV infected-PAMs using the GeneMANIA tool. (A) Significantly up-regulated proteins are shown in red; (B) Significantly down-regulated proteins are shown in blue. Edge: interaction between two different proteins.

 RT-PCR and WB Analyses of Up-Regulated IFN I-Mediated Signaling Pathways

Notably, proteins in IFN I-mediated signaling pathways were found to be up-regulated in our study. Given the importance of IFN-related proteins in host antiviral responses, we conducted RT-PCR to measure mRNA-level expression of genes that encode IFN-induced proteins, such as retinoic acid-inducible gene I (RIG-I), IFIT1, IFIT3, Mx1, Mx2, STAT1, interferon-stimulated gene 15 (ISG15), and interferon-induced transmembrane protein 1 (IFITM1) in HP-PRRSV HN07-1-infected or mock-infected PAMs. Intriguingly, abundances of mRNAs corresponding to these proteins were found to be significantly increased after HP-PRRSV HN07-1 infection (Figure 5A), with increased levels of Mx1, IFIT3, and STAT1 proteins confirmed by WB analysis (Figure 5B). Importantly, these results aligned with our MS results.

Confirmation of up-regulated proteins using RT-PCR and WB analyses. (A) PAMs were infected with HP-PRRSV HN07-1 at MOI = 0.1 or mock-infected. Samples were collected at 24 hpi for RT-PCR detection for analysis of relative mRNA abundances of RIG-I, IFIT1, IFIT3, Mx1, Mx2, ISG15, STAT1, and IFITM1. (B) PAMs were infected with HP-PRRSV at MOI = 0.1 or mock-infected. Samples were collected at 24 hpi for WB to analyze protein expression levels of STAT1, IFIT3, Mx1, and β-Actin. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.

 Decreased EIF5A Abundance After HP-PRRSV HN07-1 Infection

Translation and translational initiation related proteins were found down-regulated in the current study. The protein levels of eIF5A, eIF4E, eIF4E-binding protein 1(4EBP1) and eIF4H in HP-PRRSV HN07-1-infected PAMs at 0, 6, 12 and 24 hpi were determined by WB. The protein levels of eIF5A, 4EBP1, and eIF4H were significantly decreased after 6 hpi, whereas that of eIF4E was not significantly altered (Figure 6A). Considering that eIF5A expression was down-regulated most significantly after PRRSV HN07-1 infection along with the fact that this protein has not been extensively researched in the field of virology, we chose eIF5A as a target for further study and used eIF4E as a control. Next, we tested eIF5A dynamics after PAMs were exposed to UV-inactivated PRRSV, with the results revealing that the eIF5A level remained stable in PAMs treated with UV-inactivated virions (Figure 6B). Interestingly, transcription-level expression of eIF5A in HP-PRRSV HN07-1-infected PAMs remained almost unchanged at all time points (0, 6, 12, and 24 hpi) (Figure 6C).

The protein level of eIF5A was decreased after HP-PRRSV HN07-1 infection. (A) PAMs were infected with HP-PRRSV HN07-1 at MOI = 0.1 or mock-infected. The cells were collected at 0, 6, 12, and 24 hpi then total proteins of cells were analyzed via WB to measure protein levels of eIF5A, 4EBP1, eIF4H, and eIF4E. (B) PAMs were treated with UV-inactivated PRRSV then the cells were harvested at 0, 6, 12, and 24 hpi, then total proteins from cells were analyzed via WB to measure protein levels of eIF5A. (C) PAMs were infected with HP-PRRSV HN07-1 at MOI = 0.1 or mock-infected. The cells were collected at 0, 6, 12, and 24 hpi then total RNA preparations from the cells were analyzed via RT-PCR to detect transcription-level expression of eIF5A.

 Effect of <italic>EIF5A</italic> Knockdown on PRRSV Propagation <italic>in vitro</italic>

We next studied the biological significance of eIF5A on PRRSV infection using the CRL-2843-CD163 cell line and PAMs. CRL-2843-CD163, a cell line that stably expresses CD163, was obtained by transfection of immortalized PAMs (CRL-2843) with DNA encoding the host PRRSV receptor CD163; thus, these cells can be readily infected with PRRSV. As shown in Figure 7A, RT-RCR and WB were carried out to verify effects of eIF4E and eIF5A knockdown in CRL-2843-CD163 cells. At 24 h after transfection, RT-PCR results indicated that transcriptional expression levels of eIF5A and eIF4E were dramatically reduced. WB analysis indicated that expression levels of eIF5A and eIF4E proteins were markedly decreased at 48 h after transfection (Figure 7A).

EIF5A is important for PRRSV infection in vitro. (A) SiRNA-eIF4E and siRNA-eIF5A were transfected into CRL-2843-CD163 cells for 24, 36, or 48 h, with NC transfected as the control. The knockdown effect was validated by RT-PCR and WB. (B) The eIF4E knockdown CRL-2843-CD163 cells or PAMs were inoculated with HP-PRRSV HN07-1 (MOI = 0.1) and harvested at 12 and 24 hpi for RT-PCR analysis. (C) The eIF5A knockdown CRL-2843-CD163 cells or PAMs were inoculated with HP-PRRSV HN07-1 (MOI = 0.1) and harvested at 12 and 24 hpi for RT-PCR analysis. (D) HP-PRRSV HN07-1 (MOI = 0.1) was used to inoculate eIF5A knockdown CRL-2843-CD163 cells or PAMs and harvested at 24 hpi for IFA analysis with anti-PRRSV N protein antibody. (E) HP-PRRSV HN07-1 (MOI = 0.1) was used to inoculate eIF5A knockdown CRL-2843-CD163 cells or PAMs then cells were harvested at 24 hpi for WB analysis with anti-PRRSV N protein antibody. (F) HP-PRRSV HN07-1 (MOI = 0.1) was used to inoculate eIF5A knockdown CRL-2843-CD163 cells or PAMs then cells were harvested at 48 hpi. Viral yields were determined based on TCID50 values in MARC-145 cells. Each experiment was carried out three times independently and yielded consistent findings. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.

Next, CRL-2843-CD163 cells and PAMs were transfected with small interfering RNA (siRNA)-eIF4E and siRNA-eIF5A for 48 h, then were infected with HP-PRRSV HN07-1 and harvested at 24 hpi. Thereafter, RT-PCR was applied to verify the effects of transfected siRNAs on HP-PRRSV HN07-1 replication in vitro. The results showed that PRRSV replication in cells with knockdown of eIF4E expression (both CRL-2843-CD163 cells and PAMs) was not significantly different from that of the NC group (Figure 7B). By contrast, viral propagation in cells with knockdown of eIF5A expression was significantly inhibited (Figure 7C). In addition, expression of PRRSV N protein, as measured using IFA, was considerably suppressed in cells that were knockdown of eIF5A expression (both CRL-2843-CD163 cells and PAMs) (Figure 7D). Moreover, WB results revealed that N protein expression was dramatically reduced in cells after eIF5A knockdown (both CRL-2843-CD163 cells and PAMs) (Figure 7E). Furthermore, TCID50 values were much lower in cells after eIF5A knockdown (both CRL-2843-CD163 cells and PAMs) than in the NC group (Figure 7F).

 Recombinant EIF5A Rescue of the SiRNA-EIF5A Inhibitory Effect

To further determine whether overexpression of recombinant eIF5A could reverse the eIF5A knockdown-induced inhibitory effect on PRRSV infection, we transfected specific siRNA targeting the eIF5A 3′UTR into CRL-2843-CD163 cells to knock down endogenous eIF5A expression. Next, the cells were transfected with 3*Flag-CMV-eIF5A (Figure 8A) to restore eIF5A expression (as Flag-tagged eIF5A) while inhibiting endogenous eIF5A expression. Thereafter, the modified CRL-2843-CD163 cells were inoculated with HP-PRRSV HN07-1 (MOI = 0.1) then PRRSV propagation was assessed via RT-PCR, IFA, and WB and virus titer determinations. The results revealed that restoration of eIF5A expression in CRL-2843-CD163 cells after eIF5A knockdown rescued PRRSV propagation (Figure 8). Meanwhile, cytotoxicity assays showed that cell viability was not adversely affected by siRNA transfection or restoration of eIF5A expression (Supplementary Figures 1, 2). Taken together, all of these results demonstrated that eIF5A was required for PRRSV propagation in vitro.

Flag-tagged eIF5A rescued the inhibitory effect of eIF5A knockdown on PRRSV propagation. (A) WB analysis of endogenous eIF5A in CRL-2843-CD163 cells and recombinant Flag-tagged eIF5A in CRL-2843-CD163 cells with eIF5A knockdown. Endogenous eIF5A was knocked down by siRNA targeting of the eIF5A 3′UTR in CRL-2843-CD163 cells. HP-PRRSV HN07-1 (MOI = 0.1) was added to endogenous eIF5A knockdown CRL-2843-CD163 cells with recombinant Flag-tagged eIF5A overexpression. PRRSV propagation was validated by (B) RT-PCR, (C) IFA and (D) WB, (E) Virus titers were also determined. Each experiment was performed three times independently and all had similar results. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.

 Discussion

PAMs are known target cells of PRRSV infection. Therefore, it is of great significance to study the interaction between PRRSV and PAMs in order to clarify mechanisms involved in viral infection and propagation. Toward this goal, label-free LC-MS/MS can serve as a powerful, quantitative proteomic method that offers many advantages over traditional proteomic methods, including high sensitivity, high coverage, and high accuracy (27, 28). In our study, the proteome of HP-PRRSV HN07-1-infected PAMs was investigated using label-free LC-MS/MS, with uninfected PAMs serving as the control. Ultimately, a total of 269 differentially expressed proteins were identified, among which 46 proteins were significantly up-regulated and 223 proteins were significantly down-regulated (Tables 1, 2).

Importantly, our results revealed that up-regulated proteins were mainly enriched in IFN-I signaling pathways. RT-PCR further confirmed that specific genes associated with these pathways were significantly up-regulated at 24 hpi, such as RIG-I, IFIT1, IFIT3, Mx1, Mx2, STAT1, ISG15, and IFITM1 (Figure 5A). Moreover, expression of several these proteins (IFIT3, Mx1, and STAT1) was confirmed in HP-PRRSV HN07-1-infected PAMs at 24 hpi by WB analysis (Figure 5B). Taken together, these results indicated that PRRSV infection activated the host innate immune system, a result that was consistent with previously reported results. For example, the expression of cytoplasmic virus sensing receptors RIG-I and melanoma differentiation-associated gene five were found to be significantly increased in PRRSV-infected lungs (29). Furthermore, PRRSV infection of MARC-145 cells had been shown to up-regulate expression of Mx2, which when overexpressed was shown to suppress PRRSV replication. In addition, antiviral activity mediated by IFN-β was found to be reduced when Mx2 expression was knocked down, with Mx2 protein observed to reduce PRRSV replication through its interaction with the viral N protein (30). Importantly, infection of PAMs by PRRSV vaccine strains promoted the secretion of extracellular ISG15 from infected PAMs. This observation prompted researchers to introduce recombinant DNA encoding ISG15 into PAMs, after which PAMs expressed ISG15 then entered an antiviral state whereby PRRSV propagation was blocked (31). IFITM3 overexpression had been shown to inhibit PRRSV replication. Meanwhile, endogenous IFITM3 silencing had been shown to promote PRRSV replication. Additionally, it had also been reported that IFITM3 was S-palmitoylated and ubiquitinated and that both of these posttranslational changes contribute to the anti-PRRSV effect of IFITM3 (32).

In contrast to the results mentioned above for up-regulated proteins, down-regulated proteins were mainly enriched for functional terms related to translation-associated and translational initiation-associated processes. More specifically, levels of eIF5A, eIF5B, eIF1A, eIF4H proteins, and ribosomal proteins 40S and 60S were significantly decreased in PAMs after HP-PRRSV HN07-1-infection (Table 2). These results may be explained the host antiviral defense strategy involved the shutting down of translation-related protein synthesis to restrain virus propagation. Indeed, this concept is supported by results of numerous research studies that have shown that levels of eIFs and other host translation-related proteins were significantly decreased in cells after viral infections. For example, after infection with swine transmissible gastroenteritis virus, results of quantitative proteomic experiments revealed that expression levels of eIF3 protein and ribosomal subunit proteins 40S and 60S were considerably decreased in PK-15 cells (33). As another example, during infection with the extremely pathogenic porcine epidemic diarrhea virus (PEDV), the level of eIF2 protein was drastically decreased in Vero cells (34). As yet another example, the host translation system was shown to be repressed after PRRSV infection, with nsp2 and its transmembrane domain found to be responsible for inducing translation shutdown (35). Furthermore, an investigation of proteomic changes associated with organ infection with the PEDV YN144 strain indicated that expression levels of hnRNPA1 and eIF4G1 proteins were decreased in the PEDV YN144-infected group, suggesting that both proteins might be connected to PEDV YN144 strain pathogenicity (36).

Notably, here protein-level expression of eIF5A in PAMs infected with HP-PRRSV HN07-1 was found to be decreased via WB analysis (Figure 6), indicating that this protein might play a role in PRRSV propagation. Meanwhile, after HP-PRRSV HN07-1 infection, eIF5A protein-level expression was down-regulated in PAMs but not in PAMs exposed to UV-inactivated virions, prompting us to speculate that down-regulation of eIF5A expression in PAMs after PRRSV infection was caused by viral replication rather than viral invasion. Interestingly, transcription-level expression of eIF5A was not altered in HP-PRRSV HN07-1-infected PAMs at 0, 6, 12, and 24 hpi even though the eIF5A protein level was decreased in HP-PRRSV HN07-1-infected PAMs, warranting further study.

EIFs are important eukaryotic protein translation proteins. EIF2 inhibits protein translation, reduces the levels of early stress proteins and misfolded proteins that are produced in the endoplasmic reticulum (ER), and relieves ER stress (37). Meanwhile, eIF3 mediates ribosome binding to specific RNAs during formation of the translation initiation complex (38), while eIF4E binds to the 5′ methylated cap structure of eukaryotic mRNA and participates in the formation of the translation initiation complex (39). In fact, in recent years results of several studies have shown that eIFs are closely associated with viral replication, including results reported by Regina Cencic et al. showing that blocking the functional link between eIF4E and eIF4G greatly decreased replication of human coronavirus (40). In other studies, viral suppression of replication was observed after silencing of eIF4G1 protein expression during infections with vesicular stomatitis virus and influenza virus (41, 42).

EIF5A, which is also known as eIF4D, was first isolated from immature red blood cells (43). It is an acidic protein with a molecular mass of 17–21 kDa that is fairly well-conserved from yeast to humans (44). The function of eIF5A during translation has been widely studied in recent years, with results of studies showing that eIF5A binds to a region of the ribosome that is associated with its translation function, where it acts to promotes the elongation of numerous non polyproline-specific tripeptide sequences (45, 46). EIF5A also binds to 3′-terminal polyadenylation tails of eukaryotic mRNAs and plays a vital role in termination of translation (47). Another critical function of eIF5A is to mediate nucleocytoplasmic transport of mRNA and ensure the balanced distribution of mRNA in the cell nucleus and cytoplasm (48).

In a previous study, eIF5A was observed to play a pivotal role in human immunodeficiency virus (HIV) replication (49). In addition, Ruhl et al. reported that eIF5A participated in HIV replication in combination with HIV assistant factor regulator of expression of Rev protein (50). Subsequently, Hofmann et al. discovered that Rev protein shuttles back and forth between the nucleus and cytoplasm of host cells as part of its primary function, whereby it controls nuclear transport of non-spliced and incompletely spliced viral mRNAs as an eIF5A-dependent process (51). Meanwhile, mutation of eIF5A has been shown to significantly suppress mRNA nuclear export and inhibit HIV replication in vitro without affecting cell propagation and metabolic activity (52). In addition, expression of eIF5A was found to be down-regulated after Fe overload, while expression of the NEF protein of HIV was considerably down-regulated and HIV replication was reduced in vitro when eIF5A expression was reduced due to shRNA effects (53). Taken together, these results show that eIF5A plays a role in HIV replication, while eIF5A effects on PRRSV replication have not yet been reported.

Replication of the PRRSV RNA genome is a multi-step process involving the assembly of replication and transcription complexes that consist of viral and cell-derived components (54–56). In this study, we first investigated the role of eIF5A in PRRSV replication in vitro. Notably, PRRSV propagation was significantly inhibited after knockdown of eIF5A expression in CRL-2843-CD163 cells and PAMs even though eIF4E knockdown did not affect viral propagation (Figure 7). However, suppression of HP-PRRSV infection after eIF5A knockdown could be reversed by restoration of host cell expression of eIF5A (Figure 8). Although these results are intriguing, they raise additional questions regarding the mechanisms underlying eIF5A involvement in PRRSV propagation. Thus, experiments are currently underway in our laboratory to answer these questions toward the development of novel anti-viral strategies and more effective anti-viral drugs to combat PRRSV.

 Conclusion

In summary, here dynamic changes in the proteome of HP-PRRSV HN07-1-infected PAMs were analyzed using label-free LC-MS/MS techniques, resulting in identification of a total of 269 significantly differentially expressed host proteins. Interestingly, expression of one of these proteins, eIF5A, was down-regulated in PAMs after HP-PRRSV HN07-1 infection, while PRRSV replication was significantly inhibited and the viral titer was suppressed considerably by eIF5A knockdown in vitro. Taken together, these results demonstrated that eIF5A participates in PRRSV infection and created a foundation for further exploration of mechanisms toward the development of antiviral strategies to control and prevent PRRSV infection.

 Data Availability Statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.

 Ethics Statement

The animal study was reviewed and approved by The Ethical and Animal Welfare Committee of the Key Laboratory of Animal Immunology of the Ministry of Agriculture of China.

 Author Contributions

HL, RL, and SQ designed the experiments. HL, BW, DJ, PJ, MZ, and XL performed the experiments and analyzed the data. HL wrote the paper. RL and SQ revised the article, and all authors approved the final manuscript.

 Funding

This study was supported by grants from National Natural Science Foundation of China (31902284, 31902279), Funding scheme for young teachers in colleges and universities in Henan province (2020GGJS258), Doctoral Research Initiation Fund of Henan University of Animal Husbandry and Economy (2019HNUAHEDF040).

 Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Publisher's Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

 Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fvets.2022.861137/full#supplementary-material

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