<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3-mathml3.dtd">
<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.3" xml:lang="EN">
<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Public Health</journal-id>
<journal-title-group>
<journal-title>Frontiers in Public Health</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Public Health</abbrev-journal-title>
</journal-title-group>
<issn pub-type="epub">2296-2565</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fpubh.2026.1748436</article-id>
<article-version article-version-type="Version of Record" vocab="NISO-RP-8-2008"/>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Original Research</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Oral hygiene practices and oral health outcomes among older adults in China</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Han</surname>
<given-names>Cheng</given-names>
</name>
<xref ref-type="aff" rid="aff1"/>
<xref ref-type="corresp" rid="c001"><sup>&#x002A;</sup></xref>
<uri xlink:href="https://loop.frontiersin.org/people/3283555"/>
<role vocab="credit" vocab-identifier="https://credit.niso.org/" vocab-term="Writing &#x2013; original draft" vocab-term-identifier="https://credit.niso.org/contributor-roles/writing-original-draft/">Writing &#x2013; original draft</role>
<role vocab="credit" vocab-identifier="https://credit.niso.org/" vocab-term="Data curation" vocab-term-identifier="https://credit.niso.org/contributor-roles/data-curation/">Data curation</role>
<role vocab="credit" vocab-identifier="https://credit.niso.org/" vocab-term="software" vocab-term-identifier="https://credit.niso.org/contributor-roles/software/">Software</role>
<role vocab="credit" vocab-identifier="https://credit.niso.org/" vocab-term="Formal analysis" vocab-term-identifier="https://credit.niso.org/contributor-roles/formal-analysis/">Formal analysis</role>
<role vocab="credit" vocab-identifier="https://credit.niso.org/" vocab-term="Writing &#x2013; review &#x0026; editing" vocab-term-identifier="https://credit.niso.org/contributor-roles/writing-review-editing/">Writing &#x2013; review &#x0026; editing</role>
<role vocab="credit" vocab-identifier="https://credit.niso.org/" vocab-term="conceptualization" vocab-term-identifier="https://credit.niso.org/contributor-roles/conceptualization/">Conceptualization</role>
<role vocab="credit" vocab-identifier="https://credit.niso.org/" vocab-term="methodology" vocab-term-identifier="https://credit.niso.org/contributor-roles/methodology/">Methodology</role>
</contrib>
</contrib-group>
<aff id="aff1"><institution>Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University</institution>, <city>Nanjing</city>, <country country="cn">China</country></aff>
<author-notes>
<corresp id="c001"><label>&#x002A;</label>Correspondence: Cheng Han, <email xlink:href="mailto:17834896663@163.com">17834896663@163.com</email></corresp>
</author-notes>
<pub-date publication-format="electronic" date-type="pub" iso-8601-date="2026-02-09">
<day>09</day>
<month>02</month>
<year>2026</year>
</pub-date>
<pub-date publication-format="electronic" date-type="collection">
<year>2026</year>
</pub-date>
<volume>14</volume>
<elocation-id>1748436</elocation-id>
<history>
<date date-type="received">
<day>17</day>
<month>11</month>
<year>2025</year>
</date>
<date date-type="rev-recd">
<day>20</day>
<month>01</month>
<year>2026</year>
</date>
<date date-type="accepted">
<day>26</day>
<month>01</month>
<year>2026</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2026 Han.</copyright-statement>
<copyright-year>2026</copyright-year>
<copyright-holder>Han</copyright-holder>
<license>
<ali:license_ref start_date="2026-02-09">https://creativecommons.org/licenses/by/4.0/</ali:license_ref>
<license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License (CC BY)</ext-link>. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract>
<sec>
<title>Background</title>
<p>Oral health in older adults is crucial to overall well-being. The high prevalence of dental caries, periodontitis, and tooth loss in older adults Chinese people, together with limited preventative care, highlights the need to investigate oral hygiene behaviors and their effects on oral health.</p>
</sec>
<sec>
<title>Objective</title>
<p>To retrospectively evaluate oral hygiene behaviors and their associations with oral health outcomes among older and elder adults in China.</p>
</sec>
<sec>
<title>Methods</title>
<p>A retrospective observational study was undertaken on 500 patients aged &#x2265;60&#x202F;years utilizing hospital data. Demographic characteristics, lifestyle factors, comorbidities, dental hygiene, and oral health outcomes were collected. After adjusting for covariates, chi-square tests and multivariate logistic regression were used to examine oral hygiene behaviors and oral health outcomes.</p>
</sec>
<sec>
<title>Results</title>
<p>The average age of participants was 72.4&#x202F;&#x00B1;&#x202F;7.6&#x202F;years, with 56% female. Most individuals brushed twice daily (66%), used fluoride toothpaste (80%), and had infrequent dental appointments (56% had not seen a dentist in a year). Dental caries, periodontitis, and tooth loss affected 44, 36, and 40% of subjects, respectively. Multivariate analysis showed that twice-daily toothbrushing and the use of fluoride toothpaste were associated with a significant reduction in the risk of dental caries and periodontitis. Bass or circular brushing was associated with a reduced risk of periodontitis (adjusted OR&#x202F;=&#x202F;0.63, 95% CI: 0.41&#x2013;0.97). Dental floss, regular dental checkups, and daily mouthwash were associated with reduced tooth loss, gingival bleeding, and halitosis.</p>
</sec>
<sec>
<title>Conclusion</title>
<p>Chinese seniors who practiced regular toothbrushing, flossing, and dental visits had better oral health, highlighting the need for improved oral health education and greater emphasis on flossing to support overall oral health in this population.</p>
</sec>
</abstract>
<kwd-group>
<kwd>brushing frequency</kwd>
<kwd>dental caries</kwd>
<kwd>older and older adults population</kwd>
<kwd>periodontitis</kwd>
<kwd>tooth loss</kwd>
</kwd-group>
<funding-group>
<funding-statement>The author(s) declare that financial support was not received for the research and/or publication of this article.</funding-statement>
</funding-group>
<counts>
<fig-count count="0"/>
<table-count count="5"/>
<equation-count count="0"/>
<ref-count count="37"/>
<page-count count="8"/>
<word-count count="5546"/>
</counts>
<custom-meta-group>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Aging and Public Health</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
<body>
<sec sec-type="intro" id="sec1">
<label>1</label>
<title>Introduction</title>
<p>Dental health is crucial to overall health and quality of life, particularly among older adults (<xref ref-type="bibr" rid="ref1">1</xref>). Oral health is a critical public health issue as the global geriatric population grows. Poor oral health in older adults is associated to cardiovascular disease, diabetes, malnutrition, respiratory infections, dental caries, periodontitis, tooth loss, and oral mucosal problems (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref3">3</xref>). These issues can lower the quality of life, social participation, and functioning. Older folks globally have dental difficulties. According to the WHO, almost half of seniors have periodontal disease, and 60&#x2013;90% suffer from tooth decay. 30&#x2013;50% of seniors have lost most or all of their teeth, making chewing difficult (<xref ref-type="bibr" rid="ref4">4</xref>). Chinese older people&#x2019;s untreated caries, periodontitis, and denture difficulties reflect the country&#x2019;s growing geriatric population and oral healthcare inadequacies (<xref ref-type="bibr" rid="ref5">5</xref>). Recent Chinese data suggest that senior persons have terrible oral disease loads that need research. In the 4th National Oral Health Survey, 98.0% of Chinese elders aged 65&#x2013;74 had untreated dental caries, with at least one tooth rotting, lost, or filled (DMFT&#x202F;&#x2265;&#x202F;1) (<xref ref-type="bibr" rid="ref6">6</xref>, <xref ref-type="bibr" rid="ref7">7</xref>). National statistics show 4.5% of Chinese aged people are edentulous and have not received dental prosthetic rehabilitation (<xref ref-type="bibr" rid="ref8">8</xref>). Just 2.2% of 60-year-olds received professional dental cleanings last year. Geriatric oral hygiene is important for public health, according to Chinese epidemiology. Untreated caries, periodontal disease, and tooth loss are prevalent; behavioral and clinical reasons and preventative strategies are crucial (<xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref10">10</xref>).</p>
<p>Biological variables that contribute to dental plaque production include salivary flow, which is related to aging, oral mucosal thinness, and reduced immune response (<xref ref-type="bibr" rid="ref11">11</xref>, <xref ref-type="bibr" rid="ref12">12</xref>). Infrequent toothbrushing, improper toothbrushing technique, limited use of dental floss or mouthwash, infrequent dental visits, smoking, and poor nutrition increase the risk of dental disease (<xref ref-type="bibr" rid="ref13">13</xref>). Diabetes, cardiovascular disease, dental cavities, and periodontal disease are linked to xerostomia-causing medicines (<xref ref-type="bibr" rid="ref14">14</xref>, <xref ref-type="bibr" rid="ref15">15</xref>). Economic constraints, limited dental treatment, and low health literacy contribute to poor oral hygiene in older persons. Poor oral health in the older adults affects nutrition, systemic health, and quality of life (<xref ref-type="bibr" rid="ref16">16</xref>, <xref ref-type="bibr" rid="ref17">17</xref>). Chewing difficulties and tooth loss can cause malnutrition, poor meal quality, and unexpected weight loss in older persons. Dental infections and periodontitis cause systemic inflammation, which worsens cardiovascular disease and diabetes control (<xref ref-type="bibr" rid="ref18">18</xref>, <xref ref-type="bibr" rid="ref19">19</xref>). Oral infections, speech and swallowing issues, and xerostomia lower quality of life and social participation. Older persons with poor dental hygiene get more respiratory infections, including aspiration pneumonia (<xref ref-type="bibr" rid="ref20">20</xref>). Dental checkups, flossing, brushing, and mouthwash prevent cavities and gum disease. Dentures and other prostheses require proper care to improve chewing, nutrition, and quality of life. Systemic disorders and healthy habits promote oral health (<xref ref-type="bibr" rid="ref21">21</xref>, <xref ref-type="bibr" rid="ref22">22</xref>). Oral hygiene routines are crucial to oral health, but little is known about their effects on older Chinese adults. This study will retrospectively assess oral hygiene behaviors and oral health outcomes in older Chinese adults to provide clinical and public health initiatives for this vulnerable group.</p>
</sec>
<sec sec-type="materials|methods" id="sec2">
<label>2</label>
<title>Materials and methods</title>
<sec id="sec3">
<label>2.1</label>
<title>Ethical considerations</title>
<p>This study was approved by the Ethics Committee of Nanjing Stomatological Hospital. No. NJSH-2023NL-37 used only de-identified patient data and involved no direct contact with participants. It was conducted in accordance with the Declaration of Helsinki to ensure ethical standards and confidentiality.</p>
</sec>
<sec id="sec4">
<label>2.2</label>
<title>Study design and population</title>
<p>This retrospective observational study examined older people&#x2019; dental hygiene habits and health outcomes. Electronic hospital records and patient files provided demographic, clinical, and oral health data. The study initially identified 560 older adults patients from the records of Nanjing Stomatological Hospital. After applying inclusion and exclusion criteria, a total of 500 participants were included in the final analysis. Age&#x202F;&#x2265;&#x202F;60&#x202F;years, complete medical and dental records available in the hospital database, and community-dwelling or institutionalized older adults patients who had at least one documented dental evaluation in the past year were included in this study, and those who had incomplete or missing medical/dental records. Patients with terminal illness or severe cognitive impairment affecting reliable oral health assessment, and those who had major oral or maxillofacial surgery within the past 6&#x202F;months, were excluded from the study.</p>
</sec>
<sec id="sec5">
<label>2.3</label>
<title>Data collection</title>
<p>Data were retrospectively extracted from hospital electronic records and physical patient files using a structured data collection form. Variables collected included demographic information: age, gender, educational level, marital status, living arrangement, employment/retirement status, and residence. Lifestyle factors: smoking status, alcohol consumption, and physical activity level. Comorbidities: cardiovascular disease, hypertension, diabetes mellitus, stroke, and respiratory diseases (e.g., pneumonia, COPD). Oral hygiene practices: frequency and duration of tooth brushing, brushing technique, use of fluoride toothpaste, mouthwash, dental floss, history of dentures or prosthesis, and frequency of dental visits. The structured data collecting form was based on validated instruments used in older adults to assure content validity, and two trained researchers independently assessed a subset of records for reliability and accuracy. This method ensured collected variables were valid and consistent for analysis.</p>
</sec>
<sec id="sec6">
<label>2.4</label>
<title>Data analysis</title>
<p>Data were entered and analyzed using statistical software (SPSS version 25). Descriptive statistics were generated, including mean&#x202F;&#x00B1;&#x202F;SD for continuous variables and frequencies (%) for categorical variables. Using chi-square tests for categorical variables and logistic regression models to generate ORs with 95% CIs, gender, age, comorbidities, and lifestyle factors were used to analyze oral hygiene habits and oral health outcomes.</p>
</sec>
</sec>
<sec sec-type="results" id="sec7">
<label>3</label>
<title>Results and discussion</title>
<sec id="sec8">
<label>3.1</label>
<title>Demographic and health characteristics of the study population</title>
<p>The study included 500 older adults volunteers with a mean age of 72.4&#x202F;years (SD&#x202F;=&#x202F;7.6&#x202F;years). 16% of the participants were 60&#x2013;64&#x202F;years old, 22% were 65&#x2013;69&#x202F;years old, 24% were 70&#x2013;74&#x202F;years old, 18% were 75&#x2013;79&#x202F;years old, 12% were 80&#x2013;84&#x202F;years old, and 8% were 85&#x202F;years old. The population was 44% male and 56% female; 30% of the population had no formal education, 36 % had completed elementary, 24 % had completed secondary, and 10 % had completed higher education; 6% were divorced, 30 % widowed, and 64 % married; 74% lived with family, 20 % alone, and 6 % in institutions. Lifestyle characteristics showed that 56% of individuals had never smoked, 30% had smoked in the past, and 14% smoked currently; 70% never drank, 20 % occasionally, and 10 % often; 40% were idle, 44 % somewhat active, and 16 % exercised. Three-sixths of patients had hypertension, 24% had cardiovascular disease, 16% had diabetes, 8% had strokes, and 10% had respiratory problems such as pneumonia or COPD (<xref ref-type="table" rid="tab1">Table 1</xref>).</p>
<table-wrap position="float" id="tab1">
<label>Table 1</label>
<caption>
<p>Demographic and health characteristics of study population.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Characteristic</th>
<th align="left" valign="top">Subcategory/range</th>
<th align="left" valign="top"><italic>n</italic> (%) or mean&#x202F;&#x00B1;&#x202F;SD</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" colspan="2">Total participants</td>
<td align="left" valign="top">500 (100)</td>
</tr>
<tr>
<td align="left" valign="top">Age (years)</td>
<td align="left" valign="top">Mean&#x202F;&#x00B1;&#x202F;SD</td>
<td align="left" valign="top">72.4&#x202F;&#x00B1;&#x202F;7.6</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">Age group</td>
<td align="left" valign="top">60&#x2013;64</td>
<td align="left" valign="top">80 (16)</td>
</tr>
<tr>
<td align="left" valign="top">65&#x2013;69</td>
<td align="left" valign="top">110 (22)</td>
</tr>
<tr>
<td align="left" valign="top">70&#x2013;74</td>
<td align="left" valign="top">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top">75&#x2013;79</td>
<td align="left" valign="top">90 (18)</td>
</tr>
<tr>
<td align="left" valign="top">80&#x2013;84</td>
<td align="left" valign="top">60 (12)</td>
</tr>
<tr>
<td align="left" valign="top">&#x2265;85</td>
<td align="left" valign="top">40 (8)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">Gender</td>
<td align="left" valign="top">Male</td>
<td align="left" valign="top">220 (44)</td>
</tr>
<tr>
<td align="left" valign="top">Female</td>
<td align="left" valign="top">280 (56)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Educational level</td>
<td align="left" valign="top">No formal education</td>
<td align="left" valign="top">150 (30)</td>
</tr>
<tr>
<td align="left" valign="top">Primary school</td>
<td align="left" valign="top">180 (36)</td>
</tr>
<tr>
<td align="left" valign="top">Secondary school</td>
<td align="left" valign="top">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top">Higher education</td>
<td align="left" valign="top">50 (10)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Marital status</td>
<td align="left" valign="top">Married</td>
<td align="left" valign="top">320 (64)</td>
</tr>
<tr>
<td align="left" valign="top">Widowed</td>
<td align="left" valign="top">150 (30)</td>
</tr>
<tr>
<td align="left" valign="top">Divorced/separated</td>
<td align="left" valign="top">30 (6)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Living arrangement</td>
<td align="left" valign="top">Living alone</td>
<td align="left" valign="top">100 (20)</td>
</tr>
<tr>
<td align="left" valign="top">Living with spouse/family</td>
<td align="left" valign="top">370 (74)</td>
</tr>
<tr>
<td align="left" valign="top">Living in institutional care</td>
<td align="left" valign="top">30 (6)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Employment/retirement status</td>
<td align="left" valign="top">Employed</td>
<td align="left" valign="top">60 (12)</td>
</tr>
<tr>
<td align="left" valign="top">Retired</td>
<td align="left" valign="top">410 (82)</td>
</tr>
<tr>
<td align="left" valign="top">Unemployed</td>
<td align="left" valign="top">30 (6)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">Residence</td>
<td align="left" valign="top">Urban</td>
<td align="left" valign="top">300 (60)</td>
</tr>
<tr>
<td align="left" valign="top">Rural</td>
<td align="left" valign="top">200 (40)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="9">Lifestyle factors</td>
<td align="left" valign="top">Smoking &#x2013; never</td>
<td align="left" valign="top">280 (56)</td>
</tr>
<tr>
<td align="left" valign="top">Smoking &#x2013; former</td>
<td align="left" valign="top">150 (30)</td>
</tr>
<tr>
<td align="left" valign="top">Smoking &#x2013; current</td>
<td align="left" valign="top">70 (14)</td>
</tr>
<tr>
<td align="left" valign="top">Alcohol consumption &#x2013; never</td>
<td align="left" valign="top">350 (70)</td>
</tr>
<tr>
<td align="left" valign="top">Alcohol consumption &#x2013; occasional</td>
<td align="left" valign="top">100 (20)</td>
</tr>
<tr>
<td align="left" valign="top">Alcohol consumption &#x2013; regular</td>
<td align="left" valign="top">50 (10)</td>
</tr>
<tr>
<td align="left" valign="top">Physical activity &#x2013; sedentary</td>
<td align="left" valign="top">200 (40)</td>
</tr>
<tr>
<td align="left" valign="top">Physical activity &#x2013; moderate</td>
<td align="left" valign="top">220 (44)</td>
</tr>
<tr>
<td align="left" valign="top">Physical activity &#x2013; active</td>
<td align="left" valign="top">80 (16)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="5">Comorbidities</td>
<td align="left" valign="top">Cardiovascular disease (CVD)</td>
<td align="left" valign="top">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top">Hypertension</td>
<td align="left" valign="top">180 (36)</td>
</tr>
<tr>
<td align="left" valign="top">Diabetes mellitus</td>
<td align="left" valign="top">80 (16)</td>
</tr>
<tr>
<td align="left" valign="top">Stroke</td>
<td align="left" valign="top">40 (8)</td>
</tr>
<tr>
<td align="left" valign="top">Respiratory disease (pneumonia, COPD)</td>
<td align="left" valign="top">50 (10)</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>n</italic> (%), number and percentage of participants; SD, standard deviation.</p>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec9">
<label>3.2</label>
<title>Oral hygiene practices of the study population</title>
<p>Among the 500 older persons, a wide range of oral hygiene practices was observed. Among the people who took part in the research, 66 % rinsed their mouths, 24 % brushed their teeth, and 66 % cleaned their teeth at least three times a day; 64% of the participants cleaned their teeth for 1 to 2&#x202F;min, 24 % for longer, and 12 % for shorter; 1 to 2&#x202F;min was the typical amount of time spent brushing one&#x2019;s teeth. There were 50 % of people who used the vertical/bass brushing approach, 30 % who used the horizontal technique, and 20 % who used the circular technique. Fluoride toothpaste was used by 80 % of the people who participated in the study, whereas 20 % did not use it. It was found that 24% of people used mouthwash on a regular basis, 36% used it occasionally, and 40% never used it. In addition, only 10% of people used dental floss on a regular basis, 24% used it rarely, and 66% never used it. The percentage of respondents who had visited a dentist on a regular basis in the preceding year was 44%, while the percentage of respondents who had not seen a dentist was 56%. Two-thirds of patients came in for routine examinations, while 16% came in for treatment or discomfort related to their dental health. There were 10% of respondents who said that their oral health was exceptional, 40% who said it was good, 36% who said it was fair, and 14% who said it was poor. A total of 30 % of the individuals in the study had previously utilized dentures or prostheses, whereas 70 % had not (<xref ref-type="table" rid="tab2">Table 2</xref>).</p>
<table-wrap position="float" id="tab2">
<label>Table 2</label>
<caption>
<p>Detailed oral hygiene practices.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Oral hygiene practice (<italic>n</italic>&#x202F;=&#x202F;500)</th>
<th align="left" valign="top">Subcategory/Frequency</th>
<th align="center" valign="top"><italic>n</italic> (%)</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" rowspan="3">Tooth brushing frequency</td>
<td align="left" valign="top">Once daily</td>
<td align="char" valign="top" char="(">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top">Twice daily</td>
<td align="char" valign="top" char="(">330 (66)</td>
</tr>
<tr>
<td align="left" valign="top">&#x2265;3 times daily</td>
<td align="char" valign="top" char="(">50 (10)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Tooth brushing duration</td>
<td align="left" valign="top">&#x003C;1&#x202F;min</td>
<td align="char" valign="top" char="(">60 (12)</td>
</tr>
<tr>
<td align="left" valign="top">1&#x2013;2&#x202F;min</td>
<td align="char" valign="top" char="(">320 (64)</td>
</tr>
<tr>
<td align="left" valign="top">&#x003E;2&#x202F;min</td>
<td align="char" valign="top" char="(">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Tooth brushing technique</td>
<td align="left" valign="top">Horizontal</td>
<td align="char" valign="top" char="(">150 (30)</td>
</tr>
<tr>
<td align="left" valign="top">Vertical/Bass</td>
<td align="char" valign="top" char="(">250 (50)</td>
</tr>
<tr>
<td align="left" valign="top">Circular</td>
<td align="char" valign="top" char="(">100 (20)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">Use of fluoride toothpaste</td>
<td align="left" valign="top">Yes</td>
<td align="char" valign="top" char="(">400 (80)</td>
</tr>
<tr>
<td align="left" valign="top">No</td>
<td align="char" valign="top" char="(">100 (20)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Use of mouthwash</td>
<td align="left" valign="top">Daily</td>
<td align="char" valign="top" char="(">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top">Occasionally</td>
<td align="char" valign="top" char="(">180 (36)</td>
</tr>
<tr>
<td align="left" valign="top">Never</td>
<td align="char" valign="top" char="(">200 (40)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Use of dental floss</td>
<td align="left" valign="top">Daily</td>
<td align="char" valign="top" char="(">50 (10)</td>
</tr>
<tr>
<td align="left" valign="top">Occasionally</td>
<td align="char" valign="top" char="(">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top">Never</td>
<td align="char" valign="top" char="(">330 (66)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">Regular dental visits (past year)</td>
<td align="left" valign="top">Yes</td>
<td align="char" valign="top" char="(">220 (44)</td>
</tr>
<tr>
<td align="left" valign="top">No</td>
<td align="char" valign="top" char="(">280 (56)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">Reasons for last dental visit</td>
<td align="left" valign="top">Routine check-up</td>
<td align="char" valign="top" char="(">140 (28)</td>
</tr>
<tr>
<td align="left" valign="top">Dental pain/treatment</td>
<td align="char" valign="top" char="(">80 (16)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Self-reported oral health status</td>
<td align="left" valign="top">Excellent</td>
<td align="char" valign="top" char="(">50 (10)</td>
</tr>
<tr>
<td align="left" valign="top">Good</td>
<td align="char" valign="top" char="(">200 (40)</td>
</tr>
<tr>
<td align="left" valign="top">Fair</td>
<td align="char" valign="top" char="(">180 (36)</td>
</tr>
<tr>
<td align="left" valign="top">Poor</td>
<td align="char" valign="top" char="(">70 (14)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">History of dentures/prosthesis</td>
<td align="left" valign="top">Yes</td>
<td align="char" valign="top" char="(">150 (30)</td>
</tr>
<tr>
<td align="left" valign="top">No</td>
<td align="char" valign="top" char="(">350 (70)</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>n</italic> (%), number and percentage of participants.</p>
</table-wrap-foot>
</table-wrap>
</sec>
</sec>
<sec id="sec10">
<label>4</label>
<title>Oral health outcomes among participants</title>
<p>A study of 500 older adults found that dental diseases were common. The older participants had many dental problems; 44% had dental caries, 36 % had periodontitis, and 40 % had lost teeth; 30% had gingiva hemorrhages, and 6 % had ulcers or leukoplakia. Halitosis was noted by 20% and dry mouth by 18%. Also prominent were oral health-related functioning deficits. Oral issues caused 8% trouble swallowing, 26% difficulty chewing, and 7% difficulty speaking. These were dental issues. Mouth pain reduced meal intake for 20% of respondents. Dentures were worn by 24, 16, and 8% of people. Of participants, 24% wore dentures. This study explains tooth loss and orthodontic therapy in this demographic (<xref ref-type="table" rid="tab3">Table 3</xref>).</p>
<table-wrap position="float" id="tab3">
<label>Table 3</label>
<caption>
<p>Oral health outcomes among participants.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Oral health outcome category (<italic>n</italic>&#x202F;=&#x202F;500)</th>
<th align="left" valign="top">Specific outcome/Description</th>
<th align="left" valign="top"><italic>n</italic> (%)</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" rowspan="7">Dental conditions</td>
<td align="left" valign="top">Dental caries</td>
<td align="char" valign="top" char="(">220 (44)</td>
</tr>
<tr>
<td align="left" valign="top">Periodontitis</td>
<td align="char" valign="top" char="(">180 (36)</td>
</tr>
<tr>
<td align="left" valign="top">Tooth loss (number of teeth missing &#x2265;1)</td>
<td align="char" valign="top" char="(">200 (40)</td>
</tr>
<tr>
<td align="left" valign="top">Gingival bleeding</td>
<td align="char" valign="top" char="(">150 (30)</td>
</tr>
<tr>
<td align="left" valign="top">Oral mucosal lesions (ulcers, leukoplakia)</td>
<td align="char" valign="top" char="(">30 (6)</td>
</tr>
<tr>
<td align="left" valign="top">Halitosis (self-reported)</td>
<td align="char" valign="top" char="(">100 (20)</td>
</tr>
<tr>
<td align="left" valign="top">Dry mouth/xerostomia</td>
<td align="char" valign="top" char="(">90 (18)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Functional outcomes</td>
<td align="left" valign="top">Difficulty chewing/masticatory problems</td>
<td align="char" valign="top" char="(">130 (26)</td>
</tr>
<tr>
<td align="left" valign="top">Difficulty swallowing (dysphagia)</td>
<td align="char" valign="top" char="(">40 (8)</td>
</tr>
<tr>
<td align="left" valign="top">Speech difficulties due to dental issues</td>
<td align="char" valign="top" char="(">35 (7)</td>
</tr>
<tr>
<td align="left" valign="top">Reduced dietary variety due to oral discomfort</td>
<td align="char" valign="top" char="(">100 (20)</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">Prosthetic status</td>
<td align="left" valign="top">Denture use</td>
<td align="char" valign="top" char="(">120 (24)</td>
</tr>
<tr>
<td align="left" valign="top">Partial dentures</td>
<td align="char" valign="top" char="(">80 (16)</td>
</tr>
<tr>
<td align="left" valign="top">Full dentures</td>
<td align="char" valign="top" char="(">40 (8)</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>n</italic> (%), number and percentage of participants.</p>
</table-wrap-foot>
</table-wrap>
<sec id="sec11">
<label>4.1</label>
<title>Associations between oral hygiene practices and oral health outcomes</title>
<p>Oral hygiene and oral health were found to be significantly associated, according to the findings of an investigation. It was found that brushing teeth once daily was related to a lower risk of dental caries (odds ratio&#x202F;=&#x202F;0.48, 95% confidence interval: 0.33&#x2013;0.70, <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001) and periodontitis (odds ratio&#x202F;=&#x202F;0.52, 95% confidence interval: 0.36&#x2013;0.76, <italic>p</italic>&#x202F;=&#x202F;0.001) in comparison to brushing teeth twice daily. There was a significant reduction in tooth loss with the use of dental floss (odds ratio&#x202F;=&#x202F;0.55, 95% confidence interval: 0.34&#x2013;0.87, <italic>p</italic>&#x202F;=&#x202F;0.010). The odds ratio was 0.60, the 95% confidence interval was 0.42&#x2013;0.87, and the <italic>p</italic>-value was 0.006. Regular dental visits were found to minimize gingival bleeding. Daily use of mouthwash was associated with reduced halitosis (odds ratio&#x202F;=&#x202F;0.50, 95% confidence interval: 0.30&#x2013;0.82, <italic>p</italic>&#x202F;=&#x202F;0.005). Fluoride toothpaste significantly reduced the risk of dental caries (odds ratio&#x202F;=&#x202F;0.45; 95% confidence interval, 0.28&#x2013;0.72; <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001). Bass or circular brushing reduced periodontitis risk compared to horizontal brushing (odds ratio&#x202F;=&#x202F;0.65; 95% CI, 0.42&#x2013;1.00; <italic>p</italic>&#x202F;=&#x202F;0.05). According to these research, older individuals&#x2019; oral health is closely associated to brushing, flossing, fluoride usage, and regular dental care (<xref ref-type="table" rid="tab4">Table 4</xref>).</p>
<table-wrap position="float" id="tab4">
<label>Table 4</label>
<caption>
<p>Association between oral hygiene practices and oral health outcomes.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Oral hygiene practice</th>
<th align="left" valign="top">Oral health outcome</th>
<th align="center" valign="top">OR (95% CI)</th>
<th align="center" valign="top"><italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top">Brushing twice daily vs. once</td>
<td align="left" valign="top">Dental caries</td>
<td align="char" valign="top" char="(">0.48 (0.33&#x2013;0.70)</td>
<td align="char" valign="top" char=".">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">Brushing twice daily vs. once</td>
<td align="left" valign="top">Periodontitis</td>
<td align="char" valign="top" char="(">0.52 (0.36&#x2013;0.76)</td>
<td align="char" valign="top" char=".">0.001</td>
</tr>
<tr>
<td align="left" valign="top">Use of dental floss vs. none</td>
<td align="left" valign="top">Tooth loss</td>
<td align="char" valign="top" char="(">0.55 (0.34&#x2013;0.87)</td>
<td align="char" valign="top" char=".">0.01</td>
</tr>
<tr>
<td align="left" valign="top">Regular dental visits vs. none</td>
<td align="left" valign="top">Gingival bleeding</td>
<td align="char" valign="top" char="(">0.60 (0.42&#x2013;0.87)</td>
<td align="char" valign="top" char=".">0.006</td>
</tr>
<tr>
<td align="left" valign="top">Mouthwash use (daily) vs. never</td>
<td align="left" valign="top">Halitosis</td>
<td align="char" valign="top" char="(">0.50 (0.30&#x2013;0.82)</td>
<td align="char" valign="top" char=".">0.005</td>
</tr>
<tr>
<td align="left" valign="top">Fluoride toothpaste vs. non-fluoride</td>
<td align="left" valign="top">Dental caries</td>
<td align="char" valign="top" char="(">0.45 (0.28&#x2013;0.72)</td>
<td align="char" valign="top" char=".">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">Brushing technique (Bass/circular) vs. horizontal</td>
<td align="left" valign="top">Periodontitis</td>
<td align="char" valign="top" char="(">0.65 (0.42&#x2013;1.00)</td>
<td align="char" valign="top" char=".">0.05</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>OR, odds ratio; CI, confidence interval; reference groups &#x2013; brushing once daily, no dental floss use, no regular dental visits, never using mouthwash, non-fluoride toothpaste, and horizontal brushing technique. Statistically significant associations are indicated by <italic>p</italic>-values&#x202F;&#x003C;&#x202F;0.05.</p>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec12">
<label>4.2</label>
<title>Multivariate regression analysis for oral health outcomes</title>
<p>A multivariate logistic regression was performed to identify oral health factors among older adults. Double-daily brushing was linked to lower risk of dental caries (<italic>&#x03B2;</italic>&#x202F;=&#x202F;&#x2212;0.69, SE&#x202F;=&#x202F;0.18; adjusted odds ratio&#x202F;=&#x202F;0.50, 95% confidence interval: 0.34&#x2013;0.73, <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001) and periodontitis (<italic>&#x03B2;</italic>&#x202F;=&#x202F;&#x2212;0.60, SE&#x202F;=&#x202F;0.20; adjusted odds ratio&#x202F;=&#x202F;0.55, 95% confidence interval: 0.38&#x2013;0.80, <italic>p</italic>&#x202F;=&#x202F;0.002). Fluoride toothpaste was found to significantly protect against dental caries (<italic>&#x03B2;</italic>&#x202F;=&#x202F;&#x2212;0.76, standard error&#x202F;=&#x202F;0.24; adjusted odds ratio&#x202F;=&#x202F;0.47, 95% confidence interval: 0.29&#x2013;0.75, <italic>p</italic>&#x202F;=&#x202F;0.002). A strong correlation exists between brushing techniques, particularly the Bass or circular method, and reduced incidence of periodontitis (<italic>&#x03B2;</italic>&#x202F;=&#x202F;&#x2212;0.46, SE&#x202F;=&#x202F;0.22; adjusted odds ratio&#x202F;=&#x202F;0.63, 95% confidence interval: 0.41&#x2013;0.97, <italic>p</italic>&#x202F;=&#x202F;0.04). Dental floss use reduced tooth loss risk (<italic>&#x03B2;</italic>&#x202F;=&#x202F;&#x2212;0.55, SE&#x202F;=&#x202F;0.23; adjusted odds ratio&#x202F;=&#x202F;0.58, 95% confidence interval: 0.36&#x2013;0.91, <italic>p</italic>&#x202F;=&#x202F;0.02), while regular dental visits reduced gingival bleeding risk (<italic>&#x03B2;</italic>&#x202F;=&#x202F;&#x2212;0.49, SE&#x202F;=&#x202F;0.18; adjusted odds ratio&#x202F;=&#x202F;0.61, 95% confidence interval: 0.42&#x2013;0.88, <italic>p</italic>&#x202F;=&#x202F;0.007). The study found that daily mouthwash use reduced the probability of halitosis (<italic>&#x03B2;</italic>&#x202F;=&#x202F;&#x2212;0.67, SE&#x202F;=&#x202F;0.23; adjusted odds ratio&#x202F;=&#x202F;0.51, 95% confidence interval: 0.31&#x2013;0.84, <italic>p</italic>&#x202F;=&#x202F;0.007) (<xref ref-type="table" rid="tab5">Table 5</xref>). This discovery is significant. From these findings, older persons can dramatically lower their risk of common oral health issues by practicing good oral hygiene and getting preventative dental care.</p>
<table-wrap position="float" id="tab5">
<label>Table 5</label>
<caption>
<p>Multivariate regression analysis for oral health outcomes.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Oral Health Outcome</th>
<th align="left" valign="top">Predictor</th>
<th align="center" valign="top">&#x03B2; (SE)</th>
<th align="center" valign="top">Adjusted OR (95% CI)</th>
<th align="center" valign="top"><italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="middle" rowspan="2">Dental caries</td>
<td align="left" valign="middle">Brushing twice daily vs. once</td>
<td align="center" valign="middle">&#x2212;0.69 (0.18)</td>
<td align="center" valign="middle">0.50 (0.34&#x2013;0.73)</td>
<td align="center" valign="middle">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="middle">Fluoride toothpaste use</td>
<td align="center" valign="middle">&#x2212;0.76 (0.24)</td>
<td align="center" valign="middle">0.47 (0.29&#x2013;0.75)</td>
<td align="center" valign="middle">0.002</td>
</tr>
<tr>
<td align="left" valign="middle" rowspan="2">Periodontitis</td>
<td align="left" valign="middle">Brushing technique (Bass/circular)</td>
<td align="center" valign="middle">&#x2212;0.46 (0.22)</td>
<td align="center" valign="middle">0.63 (0.41&#x2013;0.97)</td>
<td align="center" valign="middle">0.04</td>
</tr>
<tr>
<td align="left" valign="middle">Brushing twice daily vs. once</td>
<td align="center" valign="middle">&#x2212;0.60 (0.20)</td>
<td align="center" valign="middle">0.55 (0.38&#x2013;0.80)</td>
<td align="center" valign="middle">0.002</td>
</tr>
<tr>
<td align="left" valign="middle">Tooth loss</td>
<td align="left" valign="middle">Use of dental floss vs. none</td>
<td align="center" valign="middle">&#x2212;0.55 (0.23)</td>
<td align="center" valign="middle">0.58 (0.36&#x2013;0.91)</td>
<td align="center" valign="middle">0.02</td>
</tr>
<tr>
<td align="left" valign="middle">Gingival bleeding</td>
<td align="left" valign="middle">Regular dental visits vs. none</td>
<td align="center" valign="middle">&#x2212;0.49 (0.18)</td>
<td align="center" valign="middle">0.61 (0.42&#x2013;0.88)</td>
<td align="center" valign="middle">0.007</td>
</tr>
<tr>
<td align="left" valign="middle">Halitosis</td>
<td align="left" valign="middle">Mouthwash daily vs. never</td>
<td align="center" valign="middle">&#x2212;0.67 (0.23)</td>
<td align="center" valign="middle">0.51 (0.31&#x2013;0.84)</td>
<td align="center" valign="middle">0.007</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>&#x03B2;</italic>, regression coefficient; SE, standard error; OR, odds ratio; CI, confidence interval. Reference groups, brushing once daily, nonfluoride toothpaste, horizontal brushing technique, no flossing, no regular dental visits, and never using mouthwash. Statistically significant associations are indicated by <italic>p</italic>-values&#x202F;&#x003C;&#x202F;0.05.</p>
</table-wrap-foot>
</table-wrap>
</sec>
</sec>
<sec sec-type="discussion" id="sec13">
<label>5</label>
<title>Discussion</title>
<p>This study aligns with Chinese research on the older adults. Longer life expectancy among women has led to a higher proportion of women in similar research. Previous generations of Chinese older adults have had limited access to formal education, which explains their low educational attainment. This study found marital status and living arrangements similar to those reported in previous community-based surveys, suggesting that co-residence within the family may provide social and health support (<xref ref-type="bibr" rid="ref23">23</xref>). Smoking, alcohol use, and physical activity are consistent with national and regional studies of Chinese older populations, which show high sedentary behavior and low habitual exercise. Epidemiological surveys show that older persons have a significant burden of chronic non-communicable disorders such as hypertension, cardiovascular disease, and diabetes. Rural Chinese elder research reported a significant incidence of cardiometabolic disorders like hypertension (74.8%) and diabetes (14.9%), especially among women (<xref ref-type="bibr" rid="ref24">24</xref>). Another Longitudinal Healthy Longevity Survey study found that more than two-thirds of older adults had multimorbidity, and that education was a major risk factor for impairment (<xref ref-type="bibr" rid="ref25">25</xref>). Research shows that oral health examinations should be part of overall health management in this population.</p>
<p>Research in China and elsewhere indicated that seniors&#x2019; dental hygiene regimens were comparable to those of younger generations. Investigators examined participants. Although toothbrushing twice daily is the most common practice, many older Chinese adults brush their teeth less frequently or inadequately. A poll indicated that 80% of people use fluoride toothpaste. Because this statistic is slightly higher than that reported in the regional study, urban and semi-urban residents may be more aware of the benefits of fluoride toothpaste. Previous research has shown that older adults in China rarely use dental floss due to reduced manual dexterity, cost, and limited understanding of its benefits. These findings support 10% of persons flossing daily (<xref ref-type="bibr" rid="ref26">26</xref>). Professional dental visit rates (44%) are comparable to those reported in earlier studies, indicating that older Chinese adults seek dental care only when ill. Self-reported oral health status and denture use patterns also support epidemiological studies indicating a high incidence of untreated oral disease and the need for improved preventive care in this population (<xref ref-type="bibr" rid="ref27">27</xref>). In a Mexican cross-sectional study of older adults (&#x2265;60), only 3.6% used dental floss and 16.5% used mouthwash, indicating low utilization of interdental cleaning aids (<xref ref-type="bibr" rid="ref28">28</xref>). According to a study of older community residents, 42 percent washed their teeth twice daily, whereas only 36 percent flossed or brushed their teeth frequently (<xref ref-type="bibr" rid="ref29">29</xref>). Older people typically brush their teeth, but global surveys show minimal flossing and mouthwash use. This suggests the need for greater oral health education and treatment.</p>
<p>This work supports research on ageing populations in China and globally. National surveys indicate that 44% of older adults have dental caries and 36% have periodontitis. Previous Chinese research has found that many older adults persons have at least one missing tooth, typically without prosthetic replacement. 40% of participants lost teeth (<xref ref-type="bibr" rid="ref30">30</xref>). Regional studies show age-related salivary changes, systemic illnesses, and poor oral hygiene cause comparable rates of gingival bleeding (30%) and xerostomia (18%). Chewing (26%) and dietary diversity (20%) limitations among older persons, due to tooth loss and untreated oral illness, affect nutrition and quality of life (<xref ref-type="bibr" rid="ref13">13</xref>, <xref ref-type="bibr" rid="ref31">31</xref>). Other research indicates that cost and access barriers limit prosthesis rehabilitation for many older adults with tooth loss. Denture use (24% of the population) and the distribution of partial and complete dentures confirm these findings (<xref ref-type="bibr" rid="ref32">32</xref>). Numerous nations have made similar observations. According to the Swedish National Study on Ageing and Care, the prevalence of periodontitis increases with age. After investigating, researchers found. Despite regular dental appointments, nearly half of the &#x201C;old-old&#x201D; group aged 81 lost teeth and bone (<xref ref-type="bibr" rid="ref33">33</xref>). According to US studies, 68% of 65-year-olds had chronic periodontitis (<xref ref-type="bibr" rid="ref34">34</xref>). Global studies indicate that oral disease among older persons is widespread, including in China, underscoring the need for preventive measures, routine dental care, and effective prosthesis rehabilitation.</p>
<p>These relationships have been confirmed by Chinese and international aging studies. A new study shows that twice-daily fluoride toothpaste brushing decreases periodontal disease and caries. Our data support <xref ref-type="table" rid="tab4">Tables 4</xref>, <xref ref-type="table" rid="tab5">5</xref> showing twice-daily brushing lowers dental caries and periodontitis due to better plaque removal and bacterial reduction than once-daily brushing. Regular dental checkups and flossing can detect and eliminate plaque early, preventing gingival irritation and tooth loss. Interproximal plaque reduction may explain why daily dental flossing reduced tooth loss. Due to its remineralization and acid-producing bacteria suppression, fluoride toothpaste protected against dental cavities. Bass (circular) brushing may have lowered periodontitis risk via improved gingival edge cleansing. Regular dental visits reduced gingival bleeding, possibly due to inflammation identification and treatment. Halitosis may be minimized by daily mouthwash use, which lowers oral bacterial burden and volatile sulfur compounds (<xref ref-type="bibr" rid="ref21">21</xref>, <xref ref-type="bibr" rid="ref35">35</xref>). A study of US adults over 65 found that flossers had decreased incidences of periodontal disease, cavities, and tooth loss over 5 years (<xref ref-type="bibr" rid="ref36">36</xref>). In a randomized clinical trial, brushing and rinsing with mouthwash that contains cetylpyridinium chloride (CPC) may help to reduce oral malodor (halitosis) and volatile sulfur compound-producing bacteria better than brushing alone (<xref ref-type="bibr" rid="ref37">37</xref>).</p>
<sec id="sec14">
<label>5.1</label>
<title>Limitation of the study</title>
<p>This study is limited by its retrospective design, potential selection bias toward urban and semi-urban older adults, exclusion of the rural group, and a binary oral health outcome analysis that does not account for clinical severity or longitudinal change. Nutrition, socioeconomic status, and access to non-hospital dental care may have affected the reported associations.</p>
</sec>
</sec>
<sec sec-type="conclusions" id="sec15">
<label>6</label>
<title>Conclusion</title>
<p>This study demonstrates that oral hygiene behavior and preventive care substantially affect the oral health status of older Chinese adults, among whom dental caries, periodontitis, tooth loss, and functional impairments remain highly prevalent. In addition to routine dental examination and twice-daily toothbrushing with fluoride toothpaste, the findings underscore the critical role of oral health education in promoting self-care practices. Promotion of appropriate brushing techniques (e.g., circular or Bass methods), interdental cleaning, and the use of therapeutic mouthwashes can improve oral microbial control, halitosis, masticatory function, nutritional status, and overall quality of life. Public health strategies should therefore prioritize culturally appropriate oral health education, accessible preventive services, and targeted behavioral and clinical interventions to reduce the burden of oral disease and improve the well-being of older adults.</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="sec16">
<title>Data availability statement</title>
<p>The original contributions presented in the study are included in the article/<xref rid="SM1" ref-type="supplementary-material">Supplementary material</xref>, further inquiries can be directed to the corresponding author.</p>
</sec>
<sec sec-type="ethics-statement" id="sec17">
<title>Ethics statement</title>
<p>This study was approved by the Ethics Committee of Nanjing Stomatological Hospital. No. NJSH-2023NL-37. The studies were conducted in accordance with the local legislation and institutional requirements. Written informed consent for participation was not required from the participants or the participants&#x2019; legal guardians/next of kin in accordance with the national legislation and institutional requirements.</p>
</sec>
<sec sec-type="author-contributions" id="sec18">
<title>Author contributions</title>
<p>CH: Writing &#x2013; original draft, Data curation, Software, Formal analysis, Writing &#x2013; review &#x0026; editing, Conceptualization, Methodology.</p>
</sec>
<sec sec-type="COI-statement" id="sec19">
<title>Conflict of interest</title>
<p>The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="ai-statement" id="sec20">
<title>Generative AI statement</title>
<p>The author(s) declared that Generative AI was not used in the creation of this manuscript.</p>
<p>Any alternative text (alt text) provided alongside figures in this article has been generated by Frontiers with the support of artificial intelligence and reasonable efforts have been made to ensure accuracy, including review by the authors wherever possible. If you identify any issues, please contact us.</p>
</sec>
<sec sec-type="disclaimer" id="sec21">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<sec sec-type="supplementary-material" id="sec22">
<title>Supplementary material</title>
<p>The Supplementary material for this article can be found online at: <ext-link xlink:href="https://www.frontiersin.org/articles/10.3389/fpubh.2026.1748436/full#supplementary-material" ext-link-type="uri">https://www.frontiersin.org/articles/10.3389/fpubh.2026.1748436/full#supplementary-material</ext-link></p>
<supplementary-material xlink:href="Table_1.docx" id="SM1" mimetype="application/vnd.openxmlformats-officedocument.wordprocessingml.document" xmlns:xlink="http://www.w3.org/1999/xlink"/>
</sec>
<ref-list>
<title>References</title>
<ref id="ref1"><label>1.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baniasadi</surname><given-names>K</given-names></name> <name><surname>Armoon</surname><given-names>B</given-names></name> <name><surname>Higgs</surname><given-names>P</given-names></name> <name><surname>Bayat</surname><given-names>AH</given-names></name> <name><surname>Mohammadi Gharehghani</surname><given-names>MA</given-names></name> <name><surname>Hemmat</surname><given-names>M</given-names></name> <etal/></person-group>. <article-title>The Association of Oral Health Status and socio-economic determinants with Oral health-related quality of life among the elderly: a systematic review and meta-analysis</article-title>. <source>Int J Dent Hyg</source>. (<year>2021</year>) <volume>19</volume>:<fpage>153</fpage>&#x2013;<lpage>65</lpage>. doi: <pub-id pub-id-type="doi">10.1111/idh.12489</pub-id>, <pub-id pub-id-type="pmid">33523593</pub-id></mixed-citation></ref>
<ref id="ref2"><label>2.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Janto</surname><given-names>M</given-names></name> <name><surname>Iurcov</surname><given-names>R</given-names></name> <name><surname>Daina</surname><given-names>CM</given-names></name> <name><surname>Neculoiu</surname><given-names>DC</given-names></name> <name><surname>Venter</surname><given-names>AC</given-names></name> <name><surname>Badau</surname><given-names>D</given-names></name> <etal/></person-group>. <article-title>Oral health among elderly, impact on life quality, access of elderly patients to oral health services and methods to improve oral health: a narrative review</article-title>. <source>J Pers Med</source>. (<year>2022</year>) <volume>12</volume>:<fpage>372</fpage>. doi: <pub-id pub-id-type="doi">10.3390/jpm12030372</pub-id></mixed-citation></ref>
<ref id="ref3"><label>3.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Masood</surname><given-names>M</given-names></name> <name><surname>Newton</surname><given-names>T</given-names></name> <name><surname>Bakri</surname><given-names>NN</given-names></name> <name><surname>Khalid</surname><given-names>T</given-names></name> <name><surname>Masood</surname><given-names>Y</given-names></name></person-group>. <article-title>The relationship between oral health and oral health related quality of life among elderly people in United Kingdom</article-title>. <source>J Dent</source>. (<year>2017</year>) <volume>56</volume>:<fpage>78</fpage>&#x2013;<lpage>83</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.jdent.2016.11.002</pub-id>, <pub-id pub-id-type="pmid">27825838</pub-id></mixed-citation></ref>
<ref id="ref4"><label>4.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chan</surname><given-names>AKY</given-names></name> <name><surname>Tamrakar</surname><given-names>M</given-names></name> <name><surname>Jiang</surname><given-names>CM</given-names></name> <name><surname>Lo</surname><given-names>ECM</given-names></name> <name><surname>Leung</surname><given-names>KCM</given-names></name> <name><surname>Chu</surname><given-names>C-H</given-names></name></person-group>. <article-title>Common medical and dental problems of older adults: a narrative review</article-title>. <source>Geriatrics</source>. (<year>2021</year>) <volume>6</volume>:<fpage>76</fpage>. doi: <pub-id pub-id-type="doi">10.3390/geriatrics6030076</pub-id></mixed-citation></ref>
<ref id="ref5"><label>5.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jiang</surname><given-names>CM</given-names></name> <name><surname>Chu</surname><given-names>CH</given-names></name> <name><surname>Duangthip</surname><given-names>D</given-names></name> <name><surname>Ettinger</surname><given-names>RL</given-names></name> <name><surname>Hugo</surname><given-names>FN</given-names></name> <name><surname>Kettratad-Pruksapong</surname><given-names>M</given-names></name> <etal/></person-group>. <article-title>Global perspectives of oral health policies and oral healthcare schemes for older adult populations</article-title>. <source>Front Oral Health</source>. (<year>2021</year>) <volume>2</volume>:<fpage>703526</fpage>. doi: <pub-id pub-id-type="doi">10.3389/froh.2021.703526</pub-id>, <pub-id pub-id-type="pmid">35048040</pub-id></mixed-citation></ref>
<ref id="ref6"><label>6.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lu</surname><given-names>HX</given-names></name> <name><surname>Tao</surname><given-names>DY</given-names></name> <name><surname>Lo</surname><given-names>ECM</given-names></name> <name><surname>Li</surname><given-names>R</given-names></name> <name><surname>Wang</surname><given-names>X</given-names></name> <name><surname>Tai</surname><given-names>BJ</given-names></name> <etal/></person-group>. <article-title>The 4th national oral health survey in the mainland of China: background and methodology</article-title>. <source>Chin J Dent Res</source>. (<year>2018</year>) <volume>21</volume>:<fpage>161</fpage>&#x2013;<lpage>5</lpage>. doi: <pub-id pub-id-type="doi">10.3290/j.cjdr.a41079</pub-id>, <pub-id pub-id-type="pmid">30255167</pub-id></mixed-citation></ref>
<ref id="ref7"><label>7.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ou</surname><given-names>X</given-names></name> <name><surname>Zeng</surname><given-names>L</given-names></name> <name><surname>Zeng</surname><given-names>Y</given-names></name> <name><surname>Pei</surname><given-names>Y</given-names></name> <name><surname>Zhang</surname><given-names>X</given-names></name> <name><surname>Wu</surname><given-names>W</given-names></name> <etal/></person-group>. <article-title>Health behaviors and tooth retention among older adults in China: findings from the 4th Chinese national oral health survey</article-title>. <source>BMC Oral Health</source>. (<year>2022</year>) <volume>22</volume>:<fpage>285</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s12903-022-02283-2</pub-id>, <pub-id pub-id-type="pmid">35836158</pub-id></mixed-citation></ref>
<ref id="ref8"><label>8.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sun</surname><given-names>H</given-names></name> <name><surname>Du</surname><given-names>M</given-names></name> <name><surname>Tai</surname><given-names>B</given-names></name> <name><surname>Chang</surname><given-names>S</given-names></name> <name><surname>Wang</surname><given-names>Y</given-names></name> <name><surname>Jiang</surname><given-names>H</given-names></name></person-group>. <article-title>Prevalence and associated factors of periodontal conditions among 55-to 74-year-old adults in China: results from the 4th National Oral Health Survey</article-title>. <source>Clin Oral Investig</source>. (<year>2020</year>) <volume>24</volume>:<fpage>4403</fpage>&#x2013;<lpage>12</lpage>. doi: <pub-id pub-id-type="doi">10.1007/s00784-020-03306-4</pub-id>, <pub-id pub-id-type="pmid">32382923</pub-id></mixed-citation></ref>
<ref id="ref9"><label>9.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pace</surname><given-names>CC</given-names></name> <name><surname>McCullough</surname><given-names>GH</given-names></name></person-group>. <article-title>The association between oral microorgansims and aspiration pneumonia in the institutionalized elderly: review and recommendations</article-title>. <source>Dysphagia</source>. (<year>2010</year>) <volume>25</volume>:<fpage>307</fpage>&#x2013;<lpage>22</lpage>. doi: <pub-id pub-id-type="doi">10.1007/s00455-010-9298-9</pub-id></mixed-citation></ref>
<ref id="ref10"><label>10.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Palotie</surname><given-names>U</given-names></name> <name><surname>Vehkalahti</surname><given-names>MM</given-names></name> <name><surname>Varsio</surname><given-names>S</given-names></name></person-group>. <article-title>Direct restorations and enhanced caries prevention among 20-to 60-year-olds attending Helsinki City public dental service&#x2013;a register-based observation</article-title>. <source>Acta Odontol Scand</source>. (<year>2023</year>) <volume>81</volume>:<fpage>86</fpage>&#x2013;<lpage>92</lpage>. doi: <pub-id pub-id-type="doi">10.1080/00016357.2022.2085788</pub-id>, <pub-id pub-id-type="pmid">35697018</pub-id></mixed-citation></ref>
<ref id="ref11"><label>11.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Matsuoka</surname><given-names>M</given-names></name> <name><surname>Soria</surname><given-names>SA</given-names></name> <name><surname>Pires</surname><given-names>JR</given-names></name> <name><surname>Sant&#x2019;Ana</surname><given-names>ACP</given-names></name> <name><surname>Freire</surname><given-names>M</given-names></name></person-group>. <article-title>Natural and induced immune responses in oral cavity and saliva</article-title>. <source>BMC Immunol</source>. (<year>2025</year>) <volume>26</volume>:<fpage>34</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s12865-025-00713-8</pub-id>, <pub-id pub-id-type="pmid">40251519</pub-id></mixed-citation></ref>
<ref id="ref12"><label>12.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Scannapieco</surname><given-names>FA</given-names></name> <name><surname>Shay</surname><given-names>K</given-names></name></person-group>. <article-title>Oral health disparities in older adults: oral bacteria, inflammation, and aspiration pneumonia</article-title>. <source>Dent Clin</source>. (<year>2014</year>) <volume>58</volume>:<fpage>771</fpage>&#x2013;<lpage>82</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.cden.2014.06.005</pub-id>, <pub-id pub-id-type="pmid">25201541</pub-id></mixed-citation></ref>
<ref id="ref13"><label>13.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Orisa-Ubi</surname><given-names>CO</given-names></name></person-group>. <article-title>Dental health problems associated with poor oral hygiene practices and its general health implication among the elderly</article-title>. <source>Int J Health Pharm Res</source>. (<year>2020</year>) <volume>5</volume>:<fpage>46</fpage>&#x2013;<lpage>55</lpage>.</mixed-citation></ref>
<ref id="ref14"><label>14.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Geisinger</surname><given-names>ML</given-names></name> <name><surname>Ogdon</surname><given-names>D</given-names></name> <name><surname>Kaur</surname><given-names>M</given-names></name> <name><surname>Valiquette</surname><given-names>G</given-names></name> <name><surname>Geurs</surname><given-names>NC</given-names></name> <name><surname>Reddy</surname><given-names>MS</given-names></name></person-group>. <article-title>Toss the floss? Evidence-based oral hygiene recommendations for the periodontal patient in the age of &#x201C;Flossgate&#x201D;</article-title>. <source>Clin Adv Periodontics</source>. (<year>2019</year>) <volume>9</volume>:<fpage>83</fpage>&#x2013;<lpage>90</lpage>. doi: <pub-id pub-id-type="doi">10.1002/cap.10048</pub-id></mixed-citation></ref>
<ref id="ref15"><label>15.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kakkar</surname><given-names>M</given-names></name> <name><surname>de Souza Valentim</surname><given-names>EC</given-names></name> <name><surname>Barmak</surname><given-names>AB</given-names></name> <name><surname>Arany</surname><given-names>S</given-names></name></person-group>. <article-title>Potential association of anticholinergic medication intake and caries experience in young adults with xerostomia</article-title>. <source>J Dent Sci</source>. (<year>2023</year>) <volume>18</volume>:<fpage>1693</fpage>&#x2013;<lpage>8</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.jds.2023.01.019</pub-id></mixed-citation></ref>
<ref id="ref16"><label>16.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gr&#x00F6;nbeck Lind&#x00E9;n</surname><given-names>I</given-names></name> <name><surname>H&#x00E4;gglin</surname><given-names>C</given-names></name> <name><surname>Gahnberg</surname><given-names>L</given-names></name> <name><surname>Andersson</surname><given-names>P</given-names></name></person-group>. <article-title>Factors affecting older persons&#x2019; ability to manage oral hygiene: a qualitative study</article-title>. <source>JDR Clin Trans Res</source>. (<year>2017</year>) <volume>2</volume>:<fpage>223</fpage>&#x2013;<lpage>32</lpage>. doi: <pub-id pub-id-type="doi">10.1177/2380084417709267</pub-id>, <pub-id pub-id-type="pmid">30938636</pub-id></mixed-citation></ref>
<ref id="ref17"><label>17.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Poklepovic</surname><given-names>T</given-names></name> <name><surname>Worthington</surname><given-names>HV</given-names></name> <name><surname>Johnson</surname><given-names>TM</given-names></name> <name><surname>Sambunjak</surname><given-names>D</given-names></name> <name><surname>Imai</surname><given-names>P</given-names></name> <name><surname>Clarkson</surname><given-names>JE</given-names></name> <etal/></person-group>. <article-title>Interdental brushing for the prevention and control of periodontal diseases and dental caries in adults</article-title>. <source>Cochrane Database Syst Rev</source>. (<year>2013</year>) <volume>12</volume>&#x2013;<fpage>55</fpage>. doi: <pub-id pub-id-type="doi">10.1002/14651858.CD009857.pub2</pub-id></mixed-citation></ref>
<ref id="ref18"><label>18.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chan</surname><given-names>WSH</given-names></name></person-group>. <article-title>The role of oral health in the prevention of systemic diseases</article-title>. <source>Universal Lib. Med. Health Sci.</source> (<year>2024</year>) <volume>1</volume>&#x2013;<fpage>24</fpage>. doi: <pub-id pub-id-type="doi">10.70315/uloap.ulmhs.2023.0101005</pub-id></mixed-citation></ref>
<ref id="ref19"><label>19.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ijaz</surname><given-names>MH</given-names></name></person-group>. <article-title>Oral-systemic health connections: evidence linking periodontitis with cardiovascular and metabolic diseases</article-title>. <source>J Dent Care</source>. (<year>2024</year>) <volume>1</volume>:<fpage>21</fpage>&#x2013;<lpage>30</lpage>.</mixed-citation></ref>
<ref id="ref20"><label>20.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Khadka</surname><given-names>S</given-names></name> <name><surname>Khan</surname><given-names>S</given-names></name> <name><surname>King</surname><given-names>A</given-names></name> <name><surname>Goldberg</surname><given-names>LR</given-names></name> <name><surname>Crocombe</surname><given-names>L</given-names></name> <name><surname>Bettiol</surname><given-names>S</given-names></name></person-group>. <article-title>Poor oral hygiene, oral microorganisms and aspiration pneumonia risk in older people in residential aged care: a systematic review</article-title>. <source>Age Ageing</source>. (<year>2021</year>) <volume>50</volume>:<fpage>81</fpage>&#x2013;<lpage>7</lpage>. doi: <pub-id pub-id-type="doi">10.1093/ageing/afaa102</pub-id></mixed-citation></ref>
<ref id="ref21"><label>21.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Al-Nasser</surname><given-names>L</given-names></name> <name><surname>Lamster</surname><given-names>IB</given-names></name></person-group>. <article-title>Prevention and management of periodontal diseases and dental caries in the older adults</article-title>. <source>Periodontol</source>. (<year>2020</year>) <volume>84</volume>:<fpage>69</fpage>&#x2013;<lpage>83</lpage>. doi: <pub-id pub-id-type="doi">10.1111/prd.12338</pub-id>, <pub-id pub-id-type="pmid">32844424</pub-id></mixed-citation></ref>
<ref id="ref22"><label>22.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bekzod</surname><given-names>E</given-names></name></person-group>. <article-title>Advances in oral health: prevention, treatment, and systemic implications</article-title>. <source>Am J Educ Learn</source>. (<year>2025</year>) <volume>3</volume>:<fpage>1108</fpage>&#x2013;<lpage>14</lpage>. doi: <pub-id pub-id-type="doi">10.5281/zenodo.15104352</pub-id></mixed-citation></ref>
<ref id="ref23"><label>23.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>Y</given-names></name> <name><surname>Ji</surname><given-names>H</given-names></name> <name><surname>Shen</surname><given-names>Y</given-names></name> <name><surname>Liu</surname><given-names>D</given-names></name></person-group>. <article-title>Chronic disease and multimorbidity in the Chinese older adults&#x2019; population and their impact on daily living ability: a cross-sectional study of the Chinese longitudinal healthy longevity survey (CLHLS)</article-title>. <source>Arch Public Health</source>. (<year>2024</year>) <volume>82</volume>:<fpage>17</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s13690-024-01243-2</pub-id>, <pub-id pub-id-type="pmid">38303089</pub-id></mixed-citation></ref>
<ref id="ref24"><label>24.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ding</surname><given-names>L</given-names></name> <name><surname>Liang</surname><given-names>Y</given-names></name> <name><surname>Tan</surname><given-names>EC</given-names></name> <name><surname>Hu</surname><given-names>Y</given-names></name> <name><surname>Zhang</surname><given-names>C</given-names></name> <name><surname>Liu</surname><given-names>Y</given-names></name> <etal/></person-group>. <article-title>Smoking, heavy drinking, physical inactivity, and obesity among middle-aged and older adults in China: cross-sectional findings from the baseline survey of CHARLS 2011&#x2013;2012</article-title>. <source>BMC Public Health</source>. (<year>2020</year>) <volume>20</volume>:<fpage>1062</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s12889-020-08625-5</pub-id>, <pub-id pub-id-type="pmid">32631359</pub-id></mixed-citation></ref>
<ref id="ref25"><label>25.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Calder&#x00F3;n-Larra&#x00F1;aga</surname><given-names>A</given-names></name> <name><surname>Vetrano</surname><given-names>DL</given-names></name> <name><surname>Ferrucci</surname><given-names>L</given-names></name> <name><surname>Mercer</surname><given-names>SW</given-names></name> <name><surname>Marengoni</surname><given-names>A</given-names></name> <name><surname>Onder</surname><given-names>G</given-names></name> <etal/></person-group>. <article-title>Multimorbidity and functional impairment&#x2013;bidirectional interplay, synergistic effects and common pathways</article-title>. <source>J Intern Med</source>. (<year>2019</year>) <volume>285</volume>:<fpage>255</fpage>&#x2013;<lpage>71</lpage>. doi: <pub-id pub-id-type="doi">10.1111/joim.12843</pub-id></mixed-citation></ref>
<ref id="ref26"><label>26.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tian</surname><given-names>T</given-names></name> <name><surname>Yang</surname><given-names>Z</given-names></name> <name><surname>Li</surname><given-names>S</given-names></name> <name><surname>Fan</surname><given-names>W</given-names></name> <name><surname>Wu</surname><given-names>L</given-names></name> <name><surname>Li</surname><given-names>J</given-names></name> <etal/></person-group>. <article-title>Cross-sectional survey and analysis of factors influencing the prevalence of dental caries among older individuals aged 65-74 in Guangdong Province in 2021</article-title>. <source>BMC Oral Health</source>. (<year>2024</year>) <volume>24</volume>:<fpage>948</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s12903-024-04624-9</pub-id>, <pub-id pub-id-type="pmid">39152368</pub-id></mixed-citation></ref>
<ref id="ref27"><label>27.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cui</surname><given-names>Z</given-names></name> <name><surname>Wang</surname><given-names>W</given-names></name> <name><surname>Si</surname><given-names>Y</given-names></name> <name><surname>Wang</surname><given-names>X</given-names></name> <name><surname>Feng</surname><given-names>X</given-names></name> <name><surname>Tai</surname><given-names>B</given-names></name> <etal/></person-group>. <article-title>Tooth brushing with fluoridated toothpaste and associated factors among Chinese adolescents: a nationwide cross-sectional study</article-title>. <source>BMC Oral Health</source>. (<year>2023</year>) <volume>23</volume>:<fpage>765</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s12903-023-03506-w</pub-id>, <pub-id pub-id-type="pmid">37853352</pub-id></mixed-citation></ref>
<ref id="ref28"><label>28.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nguendo-Yongsi</surname><given-names>HB</given-names></name></person-group>. <article-title>Knowledge, practices and perceptions of oral hygiene in a tropical semi-urban setting (Bafia-Cameroon)</article-title>. <source>J Med Dent Sci Res</source>. (<year>2021</year>) <volume>8</volume>:<fpage>46</fpage>&#x2013;<lpage>54</lpage>.</mixed-citation></ref>
<ref id="ref29"><label>29.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Marchesan</surname><given-names>J</given-names></name> <name><surname>Byrd</surname><given-names>K</given-names></name> <name><surname>Moss</surname><given-names>K</given-names></name> <name><surname>Preisser</surname><given-names>J</given-names></name> <name><surname>Morelli</surname><given-names>T</given-names></name> <name><surname>Zandona</surname><given-names>A</given-names></name> <etal/></person-group>. <article-title>Flossing is associated with improved oral health in older adults</article-title>. <source>J Dent Res</source>. (<year>2020</year>) <volume>99</volume>:<fpage>1047</fpage>&#x2013;<lpage>53</lpage>. doi: <pub-id pub-id-type="doi">10.1177/0022034520916151</pub-id>, <pub-id pub-id-type="pmid">32321349</pub-id></mixed-citation></ref>
<ref id="ref30"><label>30.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yu</surname><given-names>Y</given-names></name></person-group>. <article-title>Healthy ageing in urban China: governing the ageing population</article-title>. <source>Geogr J</source>. (<year>2021</year>) <volume>187</volume>:<fpage>28</fpage>&#x2013;<lpage>38</lpage>. doi: <pub-id pub-id-type="doi">10.1111/geoj.12372</pub-id></mixed-citation></ref>
<ref id="ref31"><label>31.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pei</surname><given-names>X-M</given-names></name> <name><surname>Zhou</surname><given-names>L-X</given-names></name> <name><surname>Tsang</surname><given-names>M-W</given-names></name> <name><surname>Tai</surname><given-names>WC-S</given-names></name> <name><surname>Wong</surname><given-names>S-CC</given-names></name></person-group>. <article-title>The oral microbial ecosystem in age-related xerostomia: a critical review</article-title>. <source>Int J Mol Sci</source>. (<year>2024</year>) <volume>25</volume>:<fpage>12815</fpage>. doi: <pub-id pub-id-type="doi">10.3390/ijms252312815</pub-id>, <pub-id pub-id-type="pmid">39684528</pub-id></mixed-citation></ref>
<ref id="ref32"><label>32.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ennion</surname><given-names>L</given-names></name> <name><surname>Johannesson</surname><given-names>A</given-names></name></person-group>. <article-title>A qualitative study of the challenges of providing pre-prosthetic rehabilitation in rural South Africa</article-title>. <source>Prosthetics Orthot Int</source>. (<year>2018</year>) <volume>42</volume>:<fpage>179</fpage>&#x2013;<lpage>86</lpage>. doi: <pub-id pub-id-type="doi">10.1177/0309364617698520</pub-id>, <pub-id pub-id-type="pmid">28318387</pub-id></mixed-citation></ref>
<ref id="ref33"><label>33.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Holmer</surname><given-names>J</given-names></name> <name><surname>Eriksdotter</surname><given-names>M</given-names></name> <name><surname>H&#x00E4;bel</surname><given-names>H</given-names></name> <name><surname>Hed Myrberg</surname><given-names>I</given-names></name> <name><surname>Jonsson</surname><given-names>A</given-names></name> <name><surname>Pussinen</surname><given-names>PJ</given-names></name> <etal/></person-group>. <article-title>Periodontal conditions and incident dementia: a nationwide Swedish cohort study</article-title>. <source>J Periodontol</source>. (<year>2022</year>) <volume>93</volume>:<fpage>1378</fpage>&#x2013;<lpage>86</lpage>. doi: <pub-id pub-id-type="doi">10.1002/jper.21-0518</pub-id>, <pub-id pub-id-type="pmid">35099831</pub-id></mixed-citation></ref>
<ref id="ref34"><label>34.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>S&#x00F8;dal</surname><given-names>ATT</given-names></name> <name><surname>Skudutyte-Rysstad</surname><given-names>R</given-names></name> <name><surname>Diep</surname><given-names>MT</given-names></name> <name><surname>Koldsland</surname><given-names>OC</given-names></name> <name><surname>Hove</surname><given-names>LH</given-names></name></person-group>. <article-title>Periodontitis in a 65-year-old population: risk indicators and impact on oral health-related quality of life</article-title>. <source>BMC Oral Health</source>. (<year>2022</year>) <volume>22</volume>:<fpage>640</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s12903-022-02662-9</pub-id>, <pub-id pub-id-type="pmid">36566179</pub-id></mixed-citation></ref>
<ref id="ref35"><label>35.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Worthington</surname><given-names>HV</given-names></name> <name><surname>MacDonald</surname><given-names>L</given-names></name> <name><surname>Pericic</surname><given-names>TP</given-names></name> <name><surname>Sambunjak</surname><given-names>D</given-names></name> <name><surname>Johnson</surname><given-names>TM</given-names></name> <name><surname>Imai</surname><given-names>P</given-names></name> <etal/></person-group>. <article-title>Home use of interdental cleaning devices, in addition to toothbrushing, for preventing and controlling periodontal diseases and dental caries</article-title>. <source>Cochrane Database Syst Rev</source>. (<year>2019</year>) <volume>4</volume>. doi: <pub-id pub-id-type="doi">10.1002/14651858.CD012018.pub2</pub-id>, <pub-id pub-id-type="pmid">30968949</pub-id></mixed-citation></ref>
<ref id="ref36"><label>36.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Altalhi</surname><given-names>AM</given-names></name> <name><surname>Alqahtani</surname><given-names>NS</given-names></name> <name><surname>Alareefi</surname><given-names>JA</given-names></name> <name><surname>Alamri</surname><given-names>SS</given-names></name> <name><surname>Almutairi</surname><given-names>KS</given-names></name> <name><surname>Dous</surname><given-names>RAB</given-names></name> <etal/></person-group>. <article-title>A comparative review of water flossers in periodontal therapy</article-title>. <source>Cureus</source>. (<year>2023</year>) <volume>15</volume>&#x2013;<lpage>22</lpage>. doi: <pub-id pub-id-type="doi">10.7759/cureus.50162</pub-id></mixed-citation></ref>
<ref id="ref37"><label>37.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rahman</surname><given-names>MH</given-names></name> <name><surname>Antonio</surname><given-names>JM</given-names></name> <name><surname>Lee</surname><given-names>Y-S</given-names></name> <name><surname>Fadriquela</surname><given-names>A</given-names></name> <name><surname>Kim</surname><given-names>S-M</given-names></name> <name><surname>Han</surname><given-names>S-Y</given-names></name> <etal/></person-group>. <article-title>Oral health effect of an oral rinse containing cetylpyridinium chloride: a randomized clinical trial</article-title>. <source>Mol Cell Toxicol</source>. (<year>2024</year>) <volume>20</volume>:<fpage>671</fpage>&#x2013;<lpage>8</lpage>. doi: <pub-id pub-id-type="doi">10.1007/s13273&#x2011;023&#x2011;00385&#x2011;8</pub-id></mixed-citation></ref>
</ref-list>
<fn-group>
<fn fn-type="custom" custom-type="edited-by" id="fn0001">
<p>Edited by: <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/1996929/overview">Alice Kit Ying Chan</ext-link>, The University of Hong Kong, Hong Kong SAR, China</p>
</fn>
<fn fn-type="custom" custom-type="reviewed-by" id="fn0002">
<p>Reviewed by: <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/189034/overview">William B. Grant</ext-link>, Sunlight Nutrition and Health Research Center, United States</p>
<p><ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/3154143/overview">Girish Shelke</ext-link>, Choctaw Nation Ilc, United States</p>
</fn>
</fn-group>
</back>
</article>