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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Public Health</journal-id>
<journal-title-group>
<journal-title>Frontiers in Public Health</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Public Health</abbrev-journal-title>
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<issn pub-type="epub">2296-2565</issn>
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<publisher-name>Frontiers Media S.A.</publisher-name>
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<article-meta>
<article-id pub-id-type="doi">10.3389/fpubh.2025.1644273</article-id>
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<article-categories>
<subj-group subj-group-type="heading">
<subject>Original Research</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>A community-based Daoyin program for health promotion: effects of the Qi and mind harmonizing method on body constitution for the health of older adults</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Tsai</surname>
<given-names>Yun-Ning</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
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<contrib contrib-type="author">
<name>
<surname>Chang</surname>
<given-names>Yu-Hsin</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref>
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<contrib contrib-type="author">
<name>
<surname>Su</surname>
<given-names>Yi-Chang</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
<xref ref-type="aff" rid="aff5"><sup>5</sup></xref>
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<contrib contrib-type="author">
<name>
<surname>Lee</surname>
<given-names>Shen-Ming</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref>
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<contrib contrib-type="author">
<name>
<surname>Hsiao</surname>
<given-names>Cheng-Huan</given-names>
</name>
<xref ref-type="aff" rid="aff6"><sup>6</sup></xref>
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<contrib contrib-type="author">
<name>
<surname>Lin</surname>
<given-names>Chi-Kuei</given-names>
</name>
<xref ref-type="aff" rid="aff7"><sup>7</sup></xref>
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<contrib contrib-type="author" corresp="yes">
<name>
<surname>Lin</surname>
<given-names>Sunny Jui-Shan</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
<xref ref-type="corresp" rid="c001"><sup>&#x002A;</sup></xref>
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<aff id="aff1"><label>1</label><institution>Department of Chinese Medicine, Tri-Service General Hospital, National Defense Medical University</institution>, <city>Taipei</city>, <country country="tw">Taiwan</country></aff>
<aff id="aff2"><label>2</label><institution>ChanDer Clinic</institution>, <city>Taipei</city>, <country country="tw">Taiwan</country></aff>
<aff id="aff3"><label>3</label><institution>Graduate Institute of Medical Sciences, College of Medicine, National Defense Medical University</institution>, <city>Taipei</city>, <country country="tw">Taiwan</country></aff>
<aff id="aff4"><label>4</label><institution>Department of Statistics, Feng Chia University</institution>, <city>Taichung</city>, <country country="tw">Taiwan</country></aff>
<aff id="aff5"><label>5</label><institution>National Research Institute of Chinese Medicine, Ministry of Health and Welfare</institution>, <city>Taipei</city>, <country country="tw">Taiwan</country></aff>
<aff id="aff6"><label>6</label><institution>E-Med Biotech Inc.</institution>, <city>Taichung</city>, <country country="tw">Taiwan</country></aff>
<aff id="aff7"><label>7</label><institution>Department of Chinese Medicine, Shin Kong Wu Ho-Su Memorial Hospital</institution>, <city>Taipei</city>, <country country="tw">Taiwan</country></aff>
<author-notes>
<corresp id="c001"><label>&#x002A;</label>Correspondence: Sunny Jui-Shan Lin, <email xlink:href="mailto:taco423@ms26.hinet.net">taco423@ms26.hinet.net</email></corresp>
</author-notes>
<pub-date publication-format="electronic" date-type="pub" iso-8601-date="2026-01-05">
<day>05</day>
<month>01</month>
<year>2026</year>
</pub-date>
<pub-date publication-format="electronic" date-type="collection">
<year>2025</year>
</pub-date>
<volume>13</volume>
<elocation-id>1644273</elocation-id>
<history>
<date date-type="received">
<day>10</day>
<month>06</month>
<year>2025</year>
</date>
<date date-type="rev-recd">
<day>09</day>
<month>10</month>
<year>2025</year>
</date>
<date date-type="accepted">
<day>27</day>
<month>11</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2026 Tsai, Chang, Su, Lee, Hsiao, Lin and Lin.</copyright-statement>
<copyright-year>2026</copyright-year>
<copyright-holder>Tsai, Chang, Su, Lee, Hsiao, Lin and Lin</copyright-holder>
<license>
<ali:license_ref start_date="2026-01-05">https://creativecommons.org/licenses/by/4.0/</ali:license_ref>
<license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License (CC BY)</ext-link>. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract>
<sec id="sec1001">
<title>Introduction</title>
<p>This study assessed the potential of the Qi and Mind Harmonizing Method&#x2014;a traditional Daoyin practice&#x2014;as a community-based mind&#x2013;body intervention to improve body constitution, cardiovascular function, sleep, and psychological wellbeing in older adults.</p>
</sec>
<sec id="sec2001">
<title>Methods</title>
<p>An 8-week pre-post intervention trial was conducted at community centers. Daily practice was combined with weekly 60-min group sessions. Primary outcomes included changes in body constitution. Secondary outcomes included regional body constitution scores, brachial artery blood pressure, PSQI, HRV indicators, and BSRS-5.</p>
</sec>
<sec id="sec3001">
<title>Results</title>
<p>After 8 weeks, 90 participants showed significant improvements in body constitution: Yang deficiency (&#x2212;1.9, <italic>p</italic>&#x202F;=&#x202F;0.002), Yin deficiency (&#x2212;2.3, <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001), and phlegm stasis (&#x2212;2.2, <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001). Improvements were observed in various body regions. Systolic pressure (&#x2212;3.6 mmHg, p = 0.015), mean arterial pressure (&#x2212;1.9&#x202F;mmHg, <italic>p</italic>&#x202F;=&#x202F;0.046), and pulse pressure (&#x2212;2.4 mmHg, <italic>p</italic>&#x202F;= 0.037) decreased. Sleep quality improved (PSQI &#x2212;1, <italic>p</italic>&#x202F;= 0.002). HRV analysis showed reduced LF, LF (%), and LF/HF ratio (<italic>p</italic>&#x202F;= 0.018&#x2013;0.022), and increased HF (%) (<italic>p</italic>&#x202F;= 0.028). BSRS-5 scores improved from 3.9 to 3.2 (<italic>p</italic>&#x202F;= 0.009), indicating better psychological wellbeing.</p>
</sec>
<sec id="sec4001">
<title>Discussion</title>
<p>The results may provide preliminary support for considering the integration of Daoyin into scalable public health approaches to healthy aging.</p>
</sec>
<sec>
<title>Clinical trial registration</title>
<p>Funded by the Ministry of Health and Welfare, Taiwan, Republic of China; <ext-link ext-link-type="uri" xlink:href="https://ClinicalTrials.gov">ClinicalTrials.gov</ext-link> number, NCT03640169. Registered on July 20, 2018.</p>
</sec>
</abstract>
<kwd-group>
<kwd>public health</kwd>
<kwd>aging</kwd>
<kwd>Daoyin</kwd>
<kwd>body constitution</kwd>
<kwd>sleep</kwd>
<kwd>autonomic nervous system</kwd>
<kwd>psychological wellbeing</kwd>
</kwd-group>
<funding-group>
<award-group id="gs1">
<funding-source id="sp1">
<institution-wrap>
<institution>Ministry of Health and Welfare, Taiwan</institution>
<institution-id institution-id-type="doi" vocab="open-funder-registry" vocab-identifier="10.13039/open_funder_registry">10.13039/100008903</institution-id>
</institution-wrap>
</funding-source>
<award-id rid="sp1">MOHW106-CMAP-M-114-000422</award-id>
</award-group>
<funding-statement>The author(s) declared that financial support was received for this work and/or its publication. This study was funded by the Ministry of Health and Welfare, Taiwan (Republic of China); project number MOHW106-CMAP-M-114-000422.</funding-statement>
</funding-group>
<counts>
<fig-count count="2"/>
<table-count count="2"/>
<equation-count count="0"/>
<ref-count count="61"/>
<page-count count="9"/>
<word-count count="7031"/>
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<custom-meta-group>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Aging and Public Health</meta-value>
</custom-meta>
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</article-meta>
</front>
<body>
<sec sec-type="intro" id="sec1">
<label>1</label>
<title>Introduction</title>
<p>Since the World Health Organization&#x2019;s first International Conference on Health Promotion in Ottawa in 1986, and through subsequent global health promotion conferences&#x2014;including the 9th Global Conference in Shanghai (2016)&#x2014;WHO has continued to refine and expand the principles of health promotion. These evolving frameworks emphasize not only community empowerment but also public participation (<xref ref-type="bibr" rid="ref1">1</xref>, <xref ref-type="bibr" rid="ref2">2</xref>). Building on these foundations, the Ottawa Charter&#x2019;s definition of health promotion as &#x201C;the process of enabling people to increase control over, and to improve, their health&#x201D; has remained a cornerstone in global public health. Moreover, WHO&#x2019;s current strategy for Traditional, Complementary and Integrative Medicine (TCIM) (2025&#x2013;2034) emphasizes integrating evidence-based and culturally respectful complementary practices into national health systems under appropriate regulation and community engagement (<xref ref-type="bibr" rid="ref3 ref4 ref5">3&#x2013;5</xref>). In Taiwan, these principles have been expanded to include traditional Chinese concepts of preventive medicine within public health planning (<xref ref-type="bibr" rid="ref6">6</xref>), where mind&#x2013;body practices such as Daoyin are recognized as culturally embedded approaches to health promotion (<xref ref-type="bibr" rid="ref7 ref8 ref9">7&#x2013;9</xref>).</p>
<p>Daoyin exercises originated in ancient China and have been practiced for thousands of years (<xref ref-type="bibr" rid="ref10">10</xref>). The term &#x201C;Dao&#x201D; refers to the expulsion of turbid Qi and the intake of fresh air, while &#x201C;Yin&#x201D; involves physical movements to invigorate muscles and bones. This integrated practice of controlled breathing and coordinated body movement is designed to regulate internal Qi flow, promote meridian patency, and enhance musculoskeletal function (<xref ref-type="bibr" rid="ref11">11</xref>). As a core modality in traditional Chinese medicine (TCM) and preventive care, Daoyin is recognized for its therapeutic and health-promoting potential. Common forms such as Qigong and Tai Chi fall under this umbrella and are widely adopted globally as complementary practices for strengthening physical resilience and managing chronic conditions, with growing empirical support for their efficacy (<xref ref-type="bibr" rid="ref12 ref13 ref14 ref15 ref16 ref17">12&#x2013;17</xref>). The practice of Daoyin is thus hypothesized to contribute to systemic physiological regulation and disease prevention.</p>
<p>Constitutional medicine, a cross-cultural field of study that emerged as a distinct discipline in the 19th century (<xref ref-type="bibr" rid="ref18 ref19 ref20 ref21">18&#x2013;21</xref>), continues to evolve through integration with contemporary health science (<xref ref-type="bibr" rid="ref22 ref23 ref24 ref25 ref26">22&#x2013;26</xref>). Broadly defined, constitution refers to relatively stable individual characteristics&#x2014;shaped by both genetic predisposition and environmental influences&#x2014;that affect health resilience, disease vulnerability, and physiological functioning. These characteristics include physical structure, energy regulation, and emotional tendencies. Among them, vital energy denotes the dynamic integration of energy and material substrates required for sustaining bodily function. This perspective aligns with the &#x201C;narrow definition of constitution, &#x201C;which focuses on systemic physiological regulation and serves as the conceptual framework for this study (<xref ref-type="bibr" rid="ref27">27</xref>).</p>
<p>In Traditional Chinese Medicine (TCM), human life is conceptualized as an interplay between energy (Yang, representing physiological activity) and matter (Yin, the material substrate of the body). Optimal health is thought to depend on the dynamic balance and integration of these two forces. Vital energy, or constitution, serves as a key indicator of the body&#x2019;s internal regulatory capacity and resilience. An imbalance or depletion of this vital energy may reflect transitions from health to sub-health or preclinical disease states, thereby signaling increased vulnerability to illness (<xref ref-type="bibr" rid="ref28">28</xref>, <xref ref-type="bibr" rid="ref29">29</xref>). Maintaining a well-balanced vital energy is thus essential for sustaining physiological stability and preventing disease onset (<xref ref-type="bibr" rid="ref30">30</xref>, <xref ref-type="bibr" rid="ref31">31</xref>). Yang deficiency&#x201D; (Yang-Xu), &#x201C;Yin deficiency&#x201D; (Yin-Xu), and &#x201C;Stasis&#x201D; are TCM terms used to describe constitution, respectively indicating a persistent &#x201C;lack of energy,&#x201D; &#x201C;lack of matter,&#x201D; and &#x201C;accumulation of pathological substances&#x201D; owing to the obstruction in the movement of matter by energy (<xref ref-type="bibr" rid="ref32">32</xref>). A Yang deficiency constitution may present with fatigue, cold intolerance, and loose or watery stool (<xref ref-type="bibr" rid="ref33">33</xref>); a Yin deficiency constitution may present with oral ulcers, constipation, and hot flushes (<xref ref-type="bibr" rid="ref34">34</xref>); and a Stasis constitution may present with chest tightness, easy bruising, and numbness of limbs (<xref ref-type="bibr" rid="ref32">32</xref>). These three types of constitutions can appear individually or in combination. Conversely, if none of these three constitutions is present, it is considered a &#x201C;balanced constitution.&#x201D; Additionally, the distribution of energy and matter may be the same or different in different parts of the body, such as the head, chest, abdomen, and limbs (<xref ref-type="bibr" rid="ref35">35</xref>).</p>
<p>Sleep quality is closely related to health. High-quality sleep enhances immune function, improves cognitive abilities, stabilizes mood, and maintains metabolic balance. Conversely, poor sleep quality can lead to decreased immunity, cognitive decline, psychological disorders, metabolic imbalances, and cardiovascular diseases (<xref ref-type="bibr" rid="ref36">36</xref>). Blood pressure (BP) is an important indicator of cardiovascular health (<xref ref-type="bibr" rid="ref37">37</xref>). Normal BP ensures proper blood supply to the organs and tissues, thereby supporting various bodily functions. However, high BP increases the risk of cardiovascular disease (<xref ref-type="bibr" rid="ref38">38</xref>), stroke (<xref ref-type="bibr" rid="ref39">39</xref>), kidney disease (<xref ref-type="bibr" rid="ref40">40</xref>), and other health problems (<xref ref-type="bibr" rid="ref41">41</xref>). Therefore, sleep quality and BP are key indicators of concern for health promotion policies. Increasing evidence suggests that autonomic nervous system function plays a critical role in regulating these factors, with heart rate variability (HRV) emerging as a key biomarker of autonomic balance (<xref ref-type="bibr" rid="ref42">42</xref>). Policies promoting relaxation and stress resilience often target improved HRV, as lower LF/HF ratios and higher HF% are linked to better cardiovascular and mental health (<xref ref-type="bibr" rid="ref43">43</xref>). Psychological distress, assessed by the Brief Symptom Rating Scale-5 (BSRS-5), is another crucial public health indicator, with higher scores associated with anxiety, depression, and sleep disturbances. These factors highlight the need for integrative strategies addressing both physiological and psychological wellbeing (<xref ref-type="bibr" rid="ref44">44</xref>, <xref ref-type="bibr" rid="ref45">45</xref>).</p>
<p>We hypothesized that Daoyin regulates Yin and Yang through breathing and movement, enhancing autonomic stability, improving circulation, optimizing sleep, and strengthening psychological resilience. To test this, we conducted a real-world study to evaluate its effects on health indicators and public health.</p>
</sec>
<sec sec-type="methods" id="sec2">
<label>2</label>
<title>Methods</title>
<sec id="sec3">
<label>2.1</label>
<title>Study participants</title>
<p>We conducted this trial at the Community Activity Centers in Taipei City, Taiwan, from July 10, 2017, to September 30, 2017. The Institutional Review Board approved the study protocol (Case #: CMUH106-REC1-082). Participants with significant injuries or illnesses, mental disorders, positive pregnancy tests, or those planning pregnancy during the study were excluded. Eligible participants were aged 20&#x202F;years and older. All the participants provided written informed consent.</p>
</sec>
<sec id="sec4">
<label>2.2</label>
<title>Study design</title>
<p>This was a prospective, single-arm, pre&#x2013;post intervention study conducted in a real-world community setting. No randomized control group was established, as the primary objective was to assess the feasibility and health promotion effects of the intervention under naturalistic conditions. This design was chosen to align with community-based health promotion practices and maximize participant accessibility. The research process is illustrated in <xref ref-type="fig" rid="fig1">Figure 1</xref> and was conducted in accordance with the trial protocol.</p>
<fig position="float" id="fig1">
<label>Figure 1</label>
<caption>
<p>Invitation, baseline evaluation, and completion of 8-week evaluations.</p>
</caption>
<graphic xlink:href="fpubh-13-1644273-g001.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Flowchart illustrating participant progression in a study. Out of 128 invited, 28 were excluded due to lack of interest or scheduling conflicts. 100 underwent baseline evaluation; 7 were later excluded. 93 completed an 8-week Daoyin exercise project; 3 more were excluded. Finally, 90 underwent an 8-week evaluation.</alt-text>
</graphic>
</fig>
</sec>
<sec id="sec5">
<label>2.3</label>
<title>Daoyin intervention</title>
<p>A Daoyin master with 15&#x202F;years of teaching experience led the intervention, ensuring alignment with public health promotion strategies that emphasize preventive care, community engagement, and accessible wellness practices. The initial session introduced the fundamentals of Daoyin and the Qi and Mind Harmonizing Method, providing detailed explanations of key movements and principles (<xref rid="SM1" ref-type="supplementary-material">Appendix 1</xref>), followed by instructor-led practical training. The exercises were broken down into simple, repetitive components, focusing initially on breathing awareness and gentle body movements. This gradual layering allowed participants to gain familiarity without cognitive or physical overload. To support sustained participation and health literacy, participants received instructional handouts and videos for home practice and were encouraged to join weekly 60-min group sessions held at local community centers.</p>
<p>Each session reinforced the theoretical foundations, breath regulation, physical movements, and relaxation principles central to the intervention. After standardized warm-ups, participants engaged in supervised practice with real-time feedback to enhance precision and safety, which helped consolidate their practice. During the intervention period, participants were instructed to maintain regular self-directed home practice and avoid other structured exercise routines. The research team recommended at least 15&#x202F;min of daily practice using provided materials and asked participants to log their adherence (<xref rid="SM1" ref-type="supplementary-material">Appendix 2</xref>), helping to strengthen habit formation and self-awareness. This pragmatic model reflects key public health values&#x2014;accessibility, empowerment, and sustainability&#x2014;and supports its integration into community-based health promotion initiatives.</p>
</sec>
<sec id="sec6">
<label>2.4</label>
<title>Outcome measures</title>
<p>The primary outcome was the change in body constitution from baseline to the end of the 8-week intervention period. This study used the body constitution questionnaire (BCQ) (<xref rid="SM1" ref-type="supplementary-material">Appendix 3</xref>) consisting of three subscales. Some items were shared across the subscales, resulting in a total of 44. Each item was scored on a five-point Likert scale, with scores ranging from 1 (never happened) to 5 (always happened). The three subscales were as follows:</p>
<list list-type="order">
<list-item>
<p>Yang-Xu: This subscale contained 19 items, with scores ranging from 19 to 95. A Yang-Xu constitution was indicated by a total score of 31 or above (<xref ref-type="bibr" rid="ref27">27</xref>, <xref ref-type="bibr" rid="ref33">33</xref>, <xref ref-type="bibr" rid="ref46 ref47 ref48">46&#x2013;48</xref>).</p>
</list-item>
<list-item>
<p>Yin-Xu: This subscale also contained 19 items, with scores ranging from 19 to 95. A Yin-Xu constitution was indicated by a total score of 30 or above (<xref ref-type="bibr" rid="ref34">34</xref>, <xref ref-type="bibr" rid="ref47 ref48 ref49 ref50">47&#x2013;50</xref>).</p>
</list-item>
<list-item>
<p>Stasis: This subscale included 16 items with scores ranging from 16 to 80. A Stasis constitution was indicated by a total score of 27 or above (<xref ref-type="bibr" rid="ref32">32</xref>, <xref ref-type="bibr" rid="ref47">47</xref>, <xref ref-type="bibr" rid="ref48">48</xref>).</p>
</list-item>
</list>
<p>Higher scores indicate a stronger tendency towards the corresponding constitution type. For example, a higher Yang-Xu score indicates the presence of less energy. The Cronbach&#x2019;s <italic>&#x03B1;</italic> values for the three subscales were approximately 0.85&#x2013;0.88, and the overall Cronbach&#x2019;s &#x03B1; value reached 0.90 (<xref ref-type="bibr" rid="ref51">51</xref>).</p>
<p>Secondary outcomes during the 8-week intervention included the observed changes in energy and matter across different parts of the body, BP in the left brachial artery, heart rate variability (HRV), sleep quality, and psychological distress.</p>
<p>HRV was measured using the &#x201C;Wegene&#x201D; Handheld ECG Monitor (Certified by the Ministry of Health and Welfare, Medical Device Registration No. 004896). Measurements were conducted for 5&#x202F;min while participants remained in a natural, awake state, avoiding movement and intentional breath control.</p>
<p>The study utilized the Pittsburgh sleep quality index (PSQI), developed by Buysse et al., as the measurement tool (<xref ref-type="bibr" rid="ref52">52</xref>). Participants were asked to reflect on their sleep patterns over the past month, including the following seven components:</p>
<list list-type="order">
<list-item>
<p>Subjective sleep quality &#x2014; The participant&#x2019;s satisfaction with their sleep quality.</p>
</list-item>
<list-item>
<p>Sleep latency &#x2014; The amount of time it takes to fall asleep after getting into bed.</p>
</list-item>
<list-item>
<p>Sleep duration &#x2014; The actual hours of sleep achieved each night on average.</p>
</list-item>
<list-item>
<p>Habitual sleep efficiency &#x2014; The ratio of total sleep time to time spent in bed.</p>
</list-item>
<list-item>
<p>Sleep disturbances &#x2014; The frequency and severity of disturbances during sleep.</p>
</list-item>
<list-item>
<p>Use of sleeping medication &#x2014; The frequency of sleeping pill use per week.</p>
</list-item>
<list-item>
<p>Daytime dysfunction &#x2014; The level of difficulty staying awake and maintaining enthusiasm during daily activities.</p>
</list-item>
</list>
<p>The global score, which is the sum of the scores of these seven components, ranged from 0 to 21. A global score greater than 5 indicates poor sleep quality, whereas a score of 5 or less indicates good sleep quality. In summary, a lower score indicates better sleep quality (<xref ref-type="bibr" rid="ref52">52</xref>).</p>
<p>Psychological distress was assessed using the Brief Symptom Rating Scale (BSRS-5), a self-reported questionnaire completed by participants to evaluate their mental wellbeing (<xref ref-type="bibr" rid="ref44">44</xref>, <xref ref-type="bibr" rid="ref45">45</xref>).</p>
</sec>
<sec id="sec7">
<label>2.5</label>
<title>Statistical analysis</title>
<p>Descriptive statistics were used to analyze the baseline characteristics. Paired t-tests assessed changes in BCQ, PSQI, BP, HRV, and BSRS-5 values. Continuous variables are presented as means and standard deviations, whereas categorical variables are presented as frequencies and percentages. A <italic>p</italic>-value of &#x003C; 0.05 was used as the threshold for statistical significance; <italic>p</italic>-values below 0.05 were considered significant, whereas p-values of 0.05 or greater were considered insignificant. All statistical analyses were performed using R software.</p>
</sec>
</sec>
<sec sec-type="results" id="sec8">
<label>3</label>
<title>Results</title>
<p>Of the 128 individuals invited to participate in this community-based health promotion program, 100 completed the baseline assessment. During the 8-week Daoyin intervention (<xref ref-type="fig" rid="fig2">Figure 2</xref> and <xref rid="SM1" ref-type="supplementary-material">Appendix 4</xref>), seven participants withdrew due to personal reasons, yielding a program adherence rate of 93%. Among the 93 who completed the intervention, three did not complete the post-intervention assessment. Ultimately, 90 participants completed both the pre- and post-assessments, resulting in a full program completion rate of 90% (<xref ref-type="fig" rid="fig1">Figure 1</xref>). These high rates of engagement and retention underscore the feasibility and acceptability of implementing Daoyin as a community-based health promotion strategy.</p>
<fig position="float" id="fig2">
<label>Figure 2</label>
<caption>
<p>Community-based Daoyin instruction during the 8-week intervention. Participants followed guided group practice of the Qi and Mind Harmonizing Method at a local community center. Movements were synchronized with instructional video and real-time feedback from an instructor. No identifiable faces are shown.</p>
</caption>
<graphic xlink:href="fpubh-13-1644273-g002.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">A group of people performing standing exercises on colorful mats in a room. They have their hands clasped behind their heads. Exercise equipment is visible on the left, and a projected screen is at the front.</alt-text>
</graphic>
</fig>
<sec id="sec9">
<label>3.1</label>
<title>Baseline characteristics of the patients</title>
<p>The baseline characteristics of the 90 participants before the Daoyin intervention are described, including a mean age of 63.7&#x202F;years, 66% women, and a mean body mass index of 22.8(<xref ref-type="table" rid="tab1">Table 1</xref>).</p>
<table-wrap position="float" id="tab1">
<label>Table 1</label>
<caption>
<p>Baseline Characteristics of the Study Participants.&#x002A;</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Variable</th>
<th align="center" valign="top">Baseline</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top">Female sex &#x2014; no. of patients (%)</td>
<td align="center" valign="top">59 (66%)</td>
</tr>
<tr>
<td align="left" valign="top">Age&#x2014;yr&#x002A;</td>
<td align="center" valign="top">63.7&#x202F;&#x00B1;&#x202F;11.2</td>
</tr>
<tr>
<td align="left" valign="top">Body weight (kg)&#x002A;</td>
<td align="center" valign="top">58.6&#x202F;&#x00B1;&#x202F;10.7</td>
</tr>
<tr>
<td align="left" valign="top">Body height (cm)&#x002A;</td>
<td align="center" valign="top">160.0&#x202F;&#x00B1;&#x202F;7.9</td>
</tr>
<tr>
<td align="left" valign="top">Body-mass index&#x002A;<sup>,</sup><sup>#</sup></td>
<td align="center" valign="top">22.8&#x202F;&#x00B1;&#x202F;2.9</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>&#x002A; Plus&#x2013;minus values are mean&#x202F;&#x00B1;&#x202F;SD unless otherwise noted. # Body mass index is the weight in kilograms divided by the square of the height in meters.</p>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec10">
<label>3.2</label>
<title>Primary and secondary outcomes</title>
<p><xref ref-type="table" rid="tab2">Table 2</xref> presents the changes in all outcomes from baseline to the conclusion of the 8-week intervention. In terms of primary outcomes, the BCQ scores for the Yang-Xu, Yin-Xu, and Stasis constitutions decreased significantly after 8&#x202F;weeks. The specific reductions were as follows: Yang-Xu&#x202F;&#x2212;&#x202F;1.9 (95% CI, &#x2212;3.19, &#x2212;0.72; <italic>p</italic>&#x202F;=&#x202F;0.002), Yin-Xu&#x202F;&#x2212;&#x202F;2.3 (95% CI, &#x2212;3.5, &#x2212;1.1; <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001), and Stasis &#x2212;2.2 (95% CI, &#x2212;3.34, &#x2212;1.12; p&#x202F;&#x003C;&#x202F;0.001). These scores were significantly lower than those recorded 8&#x202F;weeks earlier (<xref ref-type="table" rid="tab2">Table 2</xref>). Additionally, some participants transitioned from having a Yang-Xu constitution to no Yang-Xu constitution and from having a Yin-Xu constitution to no Yin-Xu constitution.</p>
<table-wrap position="float" id="tab2">
<label>Table 2</label>
<caption>
<p>Changes in BCQ score, blood pressure, HRV, PSQI and BSRS-5.<sup>1</sup></p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Variable</th>
<th align="center" valign="top">Baseline</th>
<th align="center" valign="top">After 8-week intervention</th>
<th align="center" valign="top"><italic>P-</italic>value<sup>2</sup></th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top">BCQ+(Yang-Xu)score<sup>3</sup></td>
<td align="center" valign="top">31.2&#x202F;&#x00B1;&#x202F;8.0</td>
<td align="center" valign="top">29.3&#x202F;&#x00B1;&#x202F;7.2</td>
<td align="center" valign="top">0.002</td>
</tr>
<tr>
<td align="left" valign="top">Factor 1: Yang-Xu in the head</td>
<td align="center" valign="top">6.1&#x202F;&#x00B1;&#x202F;2.0</td>
<td align="center" valign="top">5.7&#x202F;&#x00B1;&#x202F;2.0</td>
<td align="center" valign="top">0.026</td>
</tr>
<tr>
<td align="left" valign="top">Factor 2: Yang-Xu in the chest</td>
<td align="center" valign="top">6.2&#x202F;&#x00B1;&#x202F;2.0</td>
<td align="center" valign="top">5.6&#x202F;&#x00B1;&#x202F;2.1</td>
<td align="center" valign="top">0.013</td>
</tr>
<tr>
<td align="left" valign="top">Factor 3: Yang-Xu in the four limbs</td>
<td align="center" valign="top">6.9&#x202F;&#x00B1;&#x202F;2.4</td>
<td align="center" valign="top">6.3&#x202F;&#x00B1;&#x202F;2.3</td>
<td align="center" valign="top">0.007</td>
</tr>
<tr>
<td align="left" valign="top">Factor 4: Yang-Xu in the abdominal cavity</td>
<td align="center" valign="top">6.0&#x202F;&#x00B1;&#x202F;1.5</td>
<td align="center" valign="top">5.9&#x202F;&#x00B1;&#x202F;1.5</td>
<td align="center" valign="top">0.334</td>
</tr>
<tr>
<td align="left" valign="top">Factor 5: Yang-Xu in the body surface</td>
<td align="center" valign="top">6.0&#x202F;&#x00B1;&#x202F;2.5</td>
<td align="center" valign="top">5.8&#x202F;&#x00B1;&#x202F;2.3</td>
<td align="center" valign="top">0.136</td>
</tr>
<tr>
<td align="left" valign="top">BCQ-(Yin-Xu)score<sup>4</sup></td>
<td align="center" valign="top">31.0&#x202F;&#x00B1;&#x202F;7.4</td>
<td align="center" valign="top">28.7&#x202F;&#x00B1;&#x202F;6.7</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">Factor 1: Yin-Xu in the head</td>
<td align="center" valign="top">10.0&#x202F;&#x00B1;&#x202F;2.8</td>
<td align="center" valign="top">9.0&#x202F;&#x00B1;&#x202F;2.7</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">Factor 2: Yin-Xu in the four limbs</td>
<td align="center" valign="top">6.6&#x202F;&#x00B1;&#x202F;2.3</td>
<td align="center" valign="top">5.9&#x202F;&#x00B1;&#x202F;2.0</td>
<td align="center" valign="top">0.001</td>
</tr>
<tr>
<td align="left" valign="top">Factor 3: Yin-Xu in the gastrointestinal tract</td>
<td align="center" valign="top">6.5&#x202F;&#x00B1;&#x202F;1.9</td>
<td align="center" valign="top">6.3&#x202F;&#x00B1;&#x202F;2.0</td>
<td align="center" valign="top">0.293</td>
</tr>
<tr>
<td align="left" valign="top">Factor 4: Yin-Xu in the body surface</td>
<td align="center" valign="top">4.5&#x202F;&#x00B1;&#x202F;1.5</td>
<td align="center" valign="top">4.3&#x202F;&#x00B1;&#x202F;1.3</td>
<td align="center" valign="top">0.105</td>
</tr>
<tr>
<td align="left" valign="top">Factor 5: Yin-Xu in the abdominal cavity</td>
<td align="center" valign="top">3.4&#x202F;&#x00B1;&#x202F;1.2</td>
<td align="center" valign="top">3.2&#x202F;&#x00B1;&#x202F;1.3</td>
<td align="center" valign="top">0.097</td>
</tr>
<tr>
<td align="left" valign="top">BCQs(Stasis)score<sup>5</sup></td>
<td align="center" valign="top">26.3&#x202F;&#x00B1;&#x202F;7.4</td>
<td align="center" valign="top">24.1&#x202F;&#x00B1;&#x202F;6.9</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">Factor 1: Stasis in the trunk</td>
<td align="center" valign="top">8.1&#x202F;&#x00B1;&#x202F;2.8</td>
<td align="center" valign="top">7.3&#x202F;&#x00B1;&#x202F;2.6</td>
<td align="center" valign="top">0.002</td>
</tr>
<tr>
<td align="left" valign="top">Factor 2: Stasis in the body surface</td>
<td align="center" valign="top">7.8&#x202F;&#x00B1;&#x202F;2.7</td>
<td align="center" valign="top">7.1&#x202F;&#x00B1;&#x202F;2.3</td>
<td align="center" valign="top">0.001</td>
</tr>
<tr>
<td align="left" valign="top">Factor 3: Stasis in the head</td>
<td align="center" valign="top">5.5&#x202F;&#x00B1;&#x202F;2.0</td>
<td align="center" valign="top">5.1&#x202F;&#x00B1;&#x202F;2.1</td>
<td align="center" valign="top">0.013</td>
</tr>
<tr>
<td align="left" valign="top">Factor 4: Stasis in the gastrointestinal tract</td>
<td align="center" valign="top">4.9&#x202F;&#x00B1;&#x202F;1.9</td>
<td align="center" valign="top">4.6&#x202F;&#x00B1;&#x202F;1.6</td>
<td align="center" valign="top">0.117</td>
</tr>
<tr>
<td align="left" valign="top">Blood pressure<sup>6</sup></td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">Systolic Pressure</td>
<td align="center" valign="top">131.2&#x202F;&#x00B1;&#x202F;20.5</td>
<td align="center" valign="top">127.6&#x202F;&#x00B1;&#x202F;19.1</td>
<td align="center" valign="top">0.015</td>
</tr>
<tr>
<td align="left" valign="top">Diastolic Pressure</td>
<td align="center" valign="top">76.7&#x202F;&#x00B1;&#x202F;11.7</td>
<td align="center" valign="top">75.6&#x202F;&#x00B1;&#x202F;10.9</td>
<td align="center" valign="top">0.208</td>
</tr>
<tr>
<td align="left" valign="top">Mean arterial pressure</td>
<td align="center" valign="top">94.9&#x202F;&#x00B1;&#x202F;13.8</td>
<td align="center" valign="top">93.0&#x202F;&#x00B1;&#x202F;12.5</td>
<td align="center" valign="top">0.046</td>
</tr>
<tr>
<td align="left" valign="top">Pulse Pressure</td>
<td align="center" valign="top">54.4&#x202F;&#x00B1;&#x202F;13.62</td>
<td align="center" valign="top">52.0&#x202F;&#x00B1;&#x202F;13.9</td>
<td align="center" valign="top">0.037</td>
</tr>
<tr>
<td align="left" valign="top">HRV(Heart Rate Variability)<sup>7</sup></td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">LF</td>
<td align="center" valign="top">5.0&#x202F;&#x00B1;&#x202F;1.3</td>
<td align="center" valign="top">4.7&#x202F;&#x00B1;&#x202F;1.3</td>
<td align="center" valign="top">0.022&#x002A;</td>
</tr>
<tr>
<td align="left" valign="top">HF</td>
<td align="center" valign="top">4.4&#x202F;&#x00B1;&#x202F;1.4</td>
<td align="center" valign="top">4.3&#x202F;&#x00B1;&#x202F;1.2</td>
<td align="center" valign="top">0.723</td>
</tr>
<tr>
<td align="left" valign="top">TP</td>
<td align="center" valign="top">6.5&#x202F;&#x00B1;&#x202F;1.0</td>
<td align="center" valign="top">6.3&#x202F;&#x00B1;&#x202F;1.1</td>
<td align="center" valign="top">0.164</td>
</tr>
<tr>
<td align="left" valign="top">LF(%)</td>
<td align="center" valign="top">60.4&#x202F;&#x00B1;&#x202F;16.5</td>
<td align="center" valign="top">55.2&#x202F;&#x00B1;&#x202F;19.5</td>
<td align="center" valign="top">0.022&#x002A;</td>
</tr>
<tr>
<td align="left" valign="top">HF(%)</td>
<td align="center" valign="top">33.2&#x202F;&#x00B1;&#x202F;14.7</td>
<td align="center" valign="top">37.8&#x202F;&#x00B1;&#x202F;16.9</td>
<td align="center" valign="top">0.028&#x002A;</td>
</tr>
<tr>
<td align="left" valign="top">LF/HF</td>
<td align="center" valign="top">0.7&#x202F;&#x00B1;&#x202F;0.8</td>
<td align="center" valign="top">0.4&#x202F;&#x00B1;&#x202F;0.9</td>
<td align="center" valign="top">0.018&#x002A;</td>
</tr>
<tr>
<td align="left" valign="top">PSQI(Global score)<sup>8</sup></td>
<td align="center" valign="top">8.0&#x202F;&#x00B1;&#x202F;3.83</td>
<td align="center" valign="top">7.0&#x202F;&#x00B1;&#x202F;3.3</td>
<td align="center" valign="top">0.002</td>
</tr>
<tr>
<td align="left" valign="top">Component 1: Subjective sleep quality</td>
<td align="center" valign="top">1.4&#x202F;&#x00B1;&#x202F;0.8</td>
<td align="center" valign="top">1.2&#x202F;&#x00B1;&#x202F;0.7</td>
<td align="center" valign="top">0.001</td>
</tr>
<tr>
<td align="left" valign="top">Component 2: Sleep latency</td>
<td align="center" valign="top">1.4&#x202F;&#x00B1;&#x202F;1.0</td>
<td align="center" valign="top">1.2&#x202F;&#x00B1;&#x202F;0.8</td>
<td align="center" valign="top">0.005</td>
</tr>
<tr>
<td align="left" valign="top">Component 3: Sleep duration</td>
<td align="center" valign="top">1.7&#x202F;&#x00B1;&#x202F;0.8</td>
<td align="center" valign="top">1.8&#x202F;&#x00B1;&#x202F;0.8</td>
<td align="center" valign="top">0.902</td>
</tr>
<tr>
<td align="left" valign="top">Component 4: Habitual sleep efficiency</td>
<td align="center" valign="top">1.2&#x202F;&#x00B1;&#x202F;1.1</td>
<td align="center" valign="top">0.7&#x202F;&#x00B1;&#x202F;1.1</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">Component 5: Sleep disturbance</td>
<td align="center" valign="top">1.3&#x202F;&#x00B1;&#x202F;0.5</td>
<td align="center" valign="top">1.2&#x202F;&#x00B1;&#x202F;0.5</td>
<td align="center" valign="top">0.058</td>
</tr>
<tr>
<td align="left" valign="top">Component 6: Use of sleeping medication</td>
<td align="center" valign="top">0.5&#x202F;&#x00B1;&#x202F;1.0</td>
<td align="center" valign="top">0.4&#x202F;&#x00B1;&#x202F;0.9</td>
<td align="center" valign="top">0.114</td>
</tr>
<tr>
<td align="left" valign="top">Component 7: Daytime dysfunction</td>
<td align="center" valign="top">0.6&#x202F;&#x00B1;&#x202F;0.7</td>
<td align="center" valign="top">0.5&#x202F;&#x00B1;&#x202F;0.6</td>
<td align="center" valign="top">0.181</td>
</tr>
<tr>
<td align="left" valign="top">BSRS-5(5-item Brief Symptom Rating Scale)<sup>9</sup></td>
<td align="center" valign="top">3.9&#x202F;&#x00B1;&#x202F;3.3</td>
<td align="center" valign="top">3.2&#x202F;&#x00B1;&#x202F;2.9</td>
<td align="center" valign="top">0.009&#x002A;&#x002A;</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><sup>1</sup> All values are means with 95% confidence intervals. <sup>2</sup> p-values were calculated using repeated measures analysis of variance. A <italic>p</italic>-value&#x202F;&#x003C;&#x202F;0.05 indicates a significant difference, whereas a <italic>p</italic>-value&#x202F;&#x2265;&#x202F;0.05 indicates no significant difference. <sup>3</sup> The BCQ&#x202F;+&#x202F;(Yang-Xu) score was used to assess the level of energy deficiency. Scores ranged from 5 to 95. The criterion for judging the Yang-Xu constitution was that the total score of these 19 questions should be greater than 31, with higher scores indicating more energy deficiency. <sup>4</sup> The BCQ- (Yin-Xu) score was used to assess the level of material deficiency. Scores ranged from 5 to 95. The criterion for judging the Yin-Xu constitution was that the total score of these 19 questions should be greater than 30, with higher scores indicating greater material deficiency. <sup>5</sup> The BCQs (Stasis) score was used to assess the level of material accumulation. The scores ranged from 5 to 80. The criterion for judging the stasis constitution was that the total score of these 16 questions should be greater than 27, with higher scores indicating more material accumulation. <sup>6</sup> Systolic and diastolic pressures were measured in the left brachial artery of the participants using an electronic sphygmomanometer. Mean arterial pressure was calculated using the formula: &#x201C;systolic pressure &#x002A; 1/3&#x202F;+&#x202F;diastolic pressure &#x002A; 2/3.&#x201D; Pulse pressure was calculated using the formula: &#x201C;systolic pressure &#x2013; diastolic pressure.&#x201D; <sup>7</sup> HRV (Heart rate variability) includes the following indicators. LF (low-frequency power): Reflects overall autonomic nervous system function. HF (High-Frequency Power): Represents parasympathetic nervous system activity. TP (Total Power): Measures overall heart rate variability. LF(%)&#x202F;=&#x202F;low-frequency power in normalized unit, an indicator representing the sympathetic function. HF(%): Normalized high-frequency power, associated with parasympathetic activity. LF/HF Ratio: Calculated as Ln(LF(ms<sup>2</sup>)/HF(ms<sup>2</sup>)), used to assess sympathetic activity. <sup>8</sup> Scores on the Pittsburgh sleep quality index (PSQI) ranged from 0 to 21, with higher scores indicating worse sleep quality. <sup>9</sup> BSRS-5 (5-item Brief Symptom Rating Scale) is a validated screening tool for psychological distress, assessing five core symptoms: anxiety, depression, hostility, interpersonal sensitivity, and sleep disturbance.</p>
</table-wrap-foot>
</table-wrap>
<p>In terms of secondary outcomes, the scores for Yang-Xu, Yin-Xu, and Stasis showed changes across different body regions (<xref ref-type="table" rid="tab2">Table 2</xref>). For Yang-Xu, the score decreased by 0.4 in the head (95% CI, &#x2212;0.73, &#x2212;0.05; <italic>p</italic>&#x202F;=&#x202F;0.026), by 0.6 in the chest (95% CI, &#x2212;0.93, &#x2212;0.11; <italic>p</italic>&#x202F;=&#x202F;0.013), by 0.6 in the four limbs (95% CI, &#x2212;1.1, &#x2212;0.18; <italic>p</italic>&#x202F;=&#x202F;0.007), by 0.1 in the abdominal cavity (95% CI, &#x2212;0.44, 0.15; <italic>p</italic>&#x202F;=&#x202F;0.334), and by 0.2 on the body surface (95% CI, &#x2212;0.44, 0.15; <italic>p</italic>&#x202F;=&#x202F;0.136). For Yin-Xu, the score decreased by 1.0 in the head (95% CI, &#x2212;1.37, &#x2212;0.59; <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001), by 0.7 in the four limbs (95% CI, &#x2212;1.12, &#x2212;0.28; <italic>p</italic>&#x202F;=&#x202F;0.001), by 0.2 in the gastrointestinal tract (95% CI, &#x2212;0.58, 0.18; <italic>p</italic>&#x202F;=&#x202F;0.293), by 0.2 on the body surface (95% CI, &#x2212;0.47, 0.04; <italic>p</italic>&#x202F;=&#x202F;0.105), and by 0.2 in the abdominal cavity (95% CI, &#x2212;0.46, 0.04; <italic>p</italic>&#x202F;=&#x202F;0.097). For Stasis, the score decreased by 0.8 in the trunk (95% CI, &#x2212;1.31, &#x2212;0.31; <italic>p</italic>&#x202F;=&#x202F;0.002), by 0.7 on the body surface (95% CI, &#x2212;1.16, &#x2212;0.31; p&#x202F;=&#x202F;0.001), by 0.4 in the head (95% CI, &#x2212;0.79, &#x2212;0.09; <italic>p</italic>&#x202F;=&#x202F;0.013), and by 0.3 in the gastrointestinal tract (95% CI, &#x2212;0.55, 0.06; <italic>p</italic>&#x202F;=&#x202F;0.117). These results indicate that, while the improvements in Yang-Xu, Yin-Xu, and Stasis varied across different body regions, the overall scores for these three constitutions decreased, suggesting that the Qi and mind harmonizing method had positive effects on improving these constitutions.</p>
<p>For other secondary outcomes, systolic pressure decreased by 3.6&#x202F;mmHg (from 131.2 to 127.6; <italic>p</italic>&#x202F;=&#x202F;0.015), diastolic pressure decreased by 1.1&#x202F;mmHg (from 76.7 to 75.6; <italic>p</italic>&#x202F;=&#x202F;0.208), mean arterial pressure decreased by 1.9&#x202F;mmHg (from 94.9 to 93.0; <italic>p</italic>&#x202F;=&#x202F;0.046), and pulse pressure decreased by 2.4&#x202F;mmHg (from 54.4 to 52.0; <italic>p</italic>&#x202F;=&#x202F;0.037). These results indicate significant reductions in systolic, mean arterial, and pulse pressures. In addition to blood pressure improvements, heart rate variability (HRV) showed significant changes. The low-frequency (LF) component decreased from 5.0 to 4.7 (<italic>p</italic>&#x202F;=&#x202F;0.022), while the high-frequency (HF) component remained stable (4.4 to 4.3; <italic>p</italic>&#x202F;=&#x202F;0.723). Total power (TP) slightly declined (6.5 to 6.3; <italic>p</italic>&#x202F;=&#x202F;0.164). Notably, LF percentage dropped from 60.4 to 55.2% (p&#x202F;=&#x202F;0.022), while HF percentage rose from 33.2 to 37.8% (<italic>p</italic>&#x202F;=&#x202F;0.028), reducing the LF/HF ratio from 0.7 to 0.4 (<italic>p</italic>&#x202F;=&#x202F;0.018). These results suggest the intervention enhanced parasympathetic activity and autonomic balance.</p>
<p>After 8&#x202F;weeks of practicing the Qi and mind harmonizing method, the overall sleep quality of participants, as measured by changes in PSQI scores, improved significantly, with a reduction of 1 from 8 to 7 (<italic>p</italic>&#x202F;=&#x202F;0.002). The changes in the scores of the seven components were as follows: subjective sleep quality decreased by 0.2 (from 1.4 to 1.2; <italic>p</italic>&#x202F;=&#x202F;0.001), sleep latency decreased by 0.2 (from 1.4 to 1.2; <italic>p</italic>&#x202F;=&#x202F;0.005), sleep duration increased by 0.1 (from 1.7 to 1.8; <italic>p</italic>&#x202F;=&#x202F;0.902), habitual sleep efficiency decreased by 0.5 (from 1.2 to 0.7; <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001), sleep disturbances decreased by 0.1 (from 1.3 to 1.2; <italic>p</italic>&#x202F;=&#x202F;0.058), use of sleeping medication decreased by 0.1 (from 0.5 to 0.4; <italic>p</italic>&#x202F;=&#x202F;0.114), and daytime dysfunction increased by 0.1 (from 0.6 to 0.7; <italic>p</italic>&#x202F;=&#x202F;0.181). These results indicate that the participants were more satisfied with their sleep quality, fell asleep faster after going to bed, and had a higher proportion of sleep time in their total bedtime. Psychological distress, measured by the BSRS-5 scale, significantly decreased. The total score dropped from 3.9 to 3.2 (<italic>p</italic>&#x202F;=&#x202F;0.009), indicating improved psychological wellbeing. These findings highlight the intervention&#x2019;s potential as an effective holistic health approach.</p>
</sec>
</sec>
<sec sec-type="discussion" id="sec11">
<label>4</label>
<title>Discussion</title>
<p>The results of this real-world, prospective, pre&#x2013;post intervention study suggest that the Qi and Mind Harmonizing Method, implemented as a community-based health promotion exercise, significantly improved health parameters relevant to preventive public health strategies. After 8&#x202F;weeks of consistent practice, participants showed improvements in body constitution imbalances related to energy deficiency (Yang-Xu), material deficiency (Yin-Xu), and pathological accumulation (Stasis). Several participants transitioned from Yang-Xu and Yin-Xu to balanced constitutions. Reductions in systolic blood pressure with stable mean arterial pressure indicate decreased cardiac workload without compromising organ perfusion&#x2014;key for aging populations (<xref ref-type="bibr" rid="ref53">53</xref>). HRV analysis further demonstrated enhanced parasympathetic activity and improved autonomic regulation. Notably, PSQI and BSRS-5 scores also improved, suggesting better sleep quality and reduced psychological distress&#x2014;supporting the method&#x2019;s role in holistic health promotion strategies.</p>
<p>These findings suggest that daily self-directed practice of the Qi and Mind Harmonizing Method may strengthen the body&#x2019;s functional reserves&#x2014;both in terms of vitality and physiological support&#x2014;while improving internal regulation and reducing the buildup of harmful byproducts. Such improvements contribute to a more resilient physiological state than baseline (<xref ref-type="bibr" rid="ref54 ref55 ref56">54&#x2013;56</xref>). These outcomes were measured using the Body Constitution Questionnaire (BCQ), a validated tool with established reliability. In the context of constitution theory, enhancing an individual&#x2019;s physiological foundation may reduce disease susceptibility and promote prevention (<xref ref-type="bibr" rid="ref30">30</xref>, <xref ref-type="bibr" rid="ref31">31</xref>). This is especially relevant in aging societies, where chronic illness, functional decline, and disability are growing public health concerns (<xref ref-type="bibr" rid="ref57 ref58 ref59 ref60">57&#x2013;60</xref>). By focusing on older adults, this study provides preliminary evidence supporting the method&#x2019;s feasibility as a health promotion approach that enhances baseline health status in aging populations. These findings suggest its potential to prevent or delay age-related diseases. Further large-scale and longitudinal studies are warranted to evaluate its public health value. Various types of Daoyin exercises exist, and this study employed the Qi and Mind Harmonizing Method due to its emphasis on mental relaxation, breath regulation, and joint activation. These features reflect Daoyin&#x2019;s traditional focus on calming the mind and mobilizing the body through coordinated movement and breathing. The integration of mental focus, smooth respiration, and core muscle activation may enhance autonomic function and promote deeper circulation. These physiological effects may help optimize internal transport and address imbalances associated with low energy, limited physiological reserves, and stagnant metabolic byproducts. Future research could explore underlying mechanisms at the molecular level (<xref ref-type="bibr" rid="ref61">61</xref>). Additionally, this study identified region-specific physiological responses (<xref ref-type="table" rid="tab2">Table 2</xref>), suggesting that Daoyin may offer targeted benefits depending on body region. These findings support its potential application in precision public health (<xref ref-type="bibr" rid="ref55">55</xref>).</p>
<p>Our study had some limitations. First, this research was conducted in a real-world community setting, where we promoted the Qi and mind harmonizing method and encouraged participants to continue daily practice independently after the 8-week study to cultivate health-promoting habits. As a result, no control group was established, and long-term effects were not evaluated. Second, in Chinese culture, Daoyin is known to induce physical and mental wellbeing, making placebo effects difficult to assess. This study did not further investigate placebo effects; however, we did not emphasize expected outcomes during the study. Instead, we helped participants focus on their practice and avoided disclosing specific symptoms that might be alleviated. Third, most participants were community residents who considered themselves healthy and had no serious illnesses, so this study did not assess sub-health populations. Lastly, we observed varying degrees of improvement in Yang-Xu, Yin-Xu, and Stasis across different body regions; due to limited sample size, further validation is needed. Despite these limitations, the findings offer valuable preliminary insights into the real-world applicability of mind&#x2013;body interventions in public health.</p>
<p>This study provides valuable preliminary evidence supporting the feasibility and effectiveness of Daoyin as a community-based mind&#x2013;body intervention for health promotion. To further address the study&#x2019;s limitations and strengthen the evidence base, future research should include control groups, monitor constitution changes after practice cessation, and compare with groups receiving only health education. Documenting comorbidities and increasing sample size will enhance the generalizability and validity of results. These methodological refinements are essential to verify Daoyin&#x2019;s role in modern health promotion. By improving study design and expanding target populations, future research can better define how traditional mind&#x2013;body practices contribute to sustainable public health strategies, particularly in aging societies.</p>
</sec>
<sec sec-type="conclusions" id="sec12">
<label>5</label>
<title>Conclusion</title>
<p>This study underscores the potential of the Qi and Mind Harmonizing Method as a scalable, community-based approach for national health promotion. An 8-week program significantly improved body constitution, cardiovascular health, sleep, and psychological wellbeing. These findings not only affirm the role of accessible, culturally rooted practices in supporting healthy aging but also provide real-world evidence to inform preventive public health strategies and integrative health promotion efforts.</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="sec13">
<title>Data availability statement</title>
<p>The original contributions presented in the study are included in the article/<xref rid="SM1" ref-type="supplementary-material">Supplementary material</xref>, further inquiries can be directed to the corresponding author.</p>
</sec>
<sec sec-type="ethics-statement" id="sec14">
<title>Ethics statement</title>
<p>The studies involving humans were approved by China Medical University &#x0026; Hospital, Taichung, Taiwan of Research Ethics Committee. The studies were conducted in accordance with the local legislation and institutional requirements. The participants provided their written informed consent to participate in this study.</p>
</sec>
<sec sec-type="author-contributions" id="sec15">
<title>Author contributions</title>
<p>Y-NT: Validation, Resources, Writing &#x2013; review &#x0026; editing, Writing &#x2013; original draft, Methodology, Project administration, Visualization, Investigation. Y-HC: Resources, Formal Analysis, Project administration, Investigation, Data curation, Validation, Writing &#x2013; review &#x0026; editing, Software. Y-CS: Funding acquisition, Supervision, Conceptualization, Writing &#x2013; review &#x0026; editing, Resources. S-ML: Writing &#x2013; review &#x0026; editing, Formal Analysis, Software, Validation, Data curation. C-HH: Software, Formal Analysis, Validation, Data curation, Writing &#x2013; review &#x0026; editing. C-KL: Investigation, Writing &#x2013; review &#x0026; editing, Resources. SL: Funding acquisition, Writing &#x2013; review &#x0026; editing, Methodology, Conceptualization.</p>
</sec>
<sec sec-type="COI-statement" id="sec16">
<title>Conflict of interest</title>
<p>C-HH was employed by E-Med Biotech Inc.</p>
<p>The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="ai-statement" id="sec17">
<title>Generative AI statement</title>
<p>The author(s) declared that Generative AI was not used in the creation of this manuscript.</p>
<p>Any alternative text (alt text) provided alongside figures in this article has been generated by Frontiers with the support of artificial intelligence and reasonable efforts have been made to ensure accuracy, including review by the authors wherever possible. If you identify any issues, please contact us.</p>
</sec>
<sec sec-type="disclaimer" id="sec18">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<sec sec-type="supplementary-material" id="sec19">
<title>Supplementary material</title>
<p>The Supplementary material for this article can be found online at: <ext-link xlink:href="https://www.frontiersin.org/articles/10.3389/fpubh.2025.1644273/full#supplementary-material" ext-link-type="uri">https://www.frontiersin.org/articles/10.3389/fpubh.2025.1644273/full#supplementary-material</ext-link></p>
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<supplementary-material xlink:href="Supplementary_file_2.pdf" id="SM2" mimetype="application/pdf" xmlns:xlink="http://www.w3.org/1999/xlink"/>
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</sec>
<ref-list>
<title>References</title>
<ref id="ref1"><label>1.</label><mixed-citation publication-type="other"><person-group person-group-type="author"><collab id="coll1">Organization WH</collab></person-group>. Health Promotion Geneva: WHO; <year>2024</year>. Available online at: <ext-link xlink:href="https://www.who.int/health-topics/health-promotion" ext-link-type="uri">https://www.who.int/health-topics/health-promotion</ext-link></mixed-citation></ref>
<ref id="ref2"><label>2.</label><mixed-citation publication-type="book"><person-group person-group-type="author"><collab id="coll2">Organization WH</collab></person-group> In: <person-group person-group-type="author"><collab id="coll3">WHO</collab></person-group>, editor. <source>9th global conference on health promotion: Shanghai 2016</source>. <publisher-loc>Geneva</publisher-loc>: (<year>2016</year>)</mixed-citation></ref>
<ref id="ref3"><label>3.</label><mixed-citation publication-type="other"><person-group person-group-type="author"><collab id="coll4">Organization WH</collab></person-group>. Traditional, Complementary and Integrative Medicine (Tcim) Strategy 2025 2034. Available online at: <ext-link xlink:href="https://www.who.int/health-topics/traditional-complementary-and-integrative-medicine?utm_source=chatgpt.com#tab=tab_1" ext-link-type="uri">https://www.who.int/health-topics/traditional-complementary-and-integrative-medicine?utm_source=chatgpt.com#tab=tab_1</ext-link></mixed-citation></ref>
<ref id="ref4"><label>4.</label><mixed-citation publication-type="book"><person-group person-group-type="author"><collab id="coll5">Organization WH</collab></person-group>. <source>Global Report on traditional and complementary medicine 2019</source>. <publisher-loc>Geneva</publisher-loc>: <publisher-name>WHO</publisher-name> (<year>2019</year>).</mixed-citation></ref>
<ref id="ref5"><label>5.</label><mixed-citation publication-type="other"><person-group person-group-type="author"><collab id="coll6">Organization WH</collab></person-group>. Traditional medicine: questions and answers. Available online at: <ext-link xlink:href="https://www.who.int/news-room/questions-and-answers/item/traditional-medicine" ext-link-type="uri">https://www.who.int/news-room/questions-and-answers/item/traditional-medicine</ext-link></mixed-citation></ref>
<ref id="ref6"><label>6.</label><mixed-citation publication-type="other"><person-group person-group-type="author"><collab id="coll7">MoHaW T</collab></person-group>. The 2025 National Health and Welfare Policy White Paper. 120&#x2013;1244.</mixed-citation></ref>
<ref id="ref7"><label>7.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Taukobong</surname><given-names>NP</given-names></name> <name><surname>Myezwa</surname><given-names>H</given-names></name> <name><surname>Pengpid</surname><given-names>S</given-names></name> <name><surname>Van Geertruyden</surname><given-names>JP</given-names></name></person-group>. <article-title>The degree to which physiotherapy literature includes physical activity as a component of health promotion in practice and entry level education: a scoping systematic review</article-title>. <source>Physiother Theory Pract</source>. (<year>2014</year>) <volume>30</volume>:<fpage>12</fpage>&#x2013;<lpage>9</lpage>. doi: <pub-id pub-id-type="doi">10.3109/09593985.2013.783896</pub-id>, <pub-id pub-id-type="pmid">23808941</pub-id></mixed-citation></ref>
<ref id="ref8"><label>8.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Malik</surname><given-names>SH</given-names></name> <name><surname>Blake</surname><given-names>H</given-names></name> <name><surname>Suggs</surname><given-names>LS</given-names></name></person-group>. <article-title>A systematic review of workplace health promotion interventions for increasing physical activity</article-title>. <source>Br J Health Psychol</source>. (<year>2014</year>) <volume>19</volume>:<fpage>149</fpage>&#x2013;<lpage>80</lpage>. doi: <pub-id pub-id-type="doi">10.1111/bjhp.12052</pub-id>, <pub-id pub-id-type="pmid">23827053</pub-id></mixed-citation></ref>
<ref id="ref9"><label>9.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gutermuth</surname><given-names>LK</given-names></name> <name><surname>Hager</surname><given-names>ER</given-names></name> <name><surname>Pollack Porter</surname><given-names>K</given-names></name></person-group>. <article-title>Using the Cdc's worksite health scorecard as a framework to examine worksite health promotion and physical activity</article-title>. <source>Prev Chronic Dis</source>. (<year>2018</year>) <volume>15</volume>:<fpage>E84</fpage>. doi: <pub-id pub-id-type="doi">10.5888/pcd15.170463</pub-id>, <pub-id pub-id-type="pmid">29935077</pub-id></mixed-citation></ref>
<ref id="ref10"><label>10.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>X</given-names></name> <name><surname>Cui</surname><given-names>J</given-names></name> <name><surname>Li</surname><given-names>R</given-names></name> <name><surname>Norton</surname><given-names>R</given-names></name> <name><surname>Park</surname><given-names>J</given-names></name> <name><surname>Kong</surname><given-names>J</given-names></name> <etal/></person-group>. <article-title>Dao yin (a.K.A. qigong): origin, development, potential mechanisms, and clinical applications</article-title>. <source>eCAM</source>. (<year>2019</year>) <volume>2019</volume>:<fpage>3705120</fpage>. doi: <pub-id pub-id-type="doi">10.1155/2019/3705120</pub-id>, <pub-id pub-id-type="pmid">31772593</pub-id></mixed-citation></ref>
<ref id="ref11"><label>11.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lee</surname><given-names>SH</given-names></name> <name><surname>Jeon</surname><given-names>Y</given-names></name> <name><surname>Huang</surname><given-names>CW</given-names></name> <name><surname>Cheon</surname><given-names>C</given-names></name> <name><surname>Ko</surname><given-names>SG</given-names></name></person-group>. <article-title>Qigong and tai chi on human health: an overview of systematic reviews</article-title>. <source>Am J Chin Med</source>. (<year>2022</year>) <volume>50</volume>:<fpage>1995</fpage>&#x2013;<lpage>2010</lpage>. doi: <pub-id pub-id-type="doi">10.1142/s0192415x22500859</pub-id>, <pub-id pub-id-type="pmid">36266755</pub-id></mixed-citation></ref>
<ref id="ref12"><label>12.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>F</given-names></name> <name><surname>Harmer</surname><given-names>P</given-names></name> <name><surname>Fitzgerald</surname><given-names>K</given-names></name> <name><surname>Eckstrom</surname><given-names>E</given-names></name> <name><surname>Stock</surname><given-names>R</given-names></name> <name><surname>Galver</surname><given-names>J</given-names></name> <etal/></person-group>. <article-title>Tai chi and Postural stability in patients with Parkinson's disease</article-title>. <source>N Engl J Med</source>. (<year>2012</year>) <volume>366</volume>:<fpage>511</fpage>&#x2013;<lpage>9</lpage>. doi: <pub-id pub-id-type="doi">10.1056/NEJMoa1107911</pub-id>, <pub-id pub-id-type="pmid">22316445</pub-id></mixed-citation></ref>
<ref id="ref13"><label>13.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>C</given-names></name> <name><surname>Schmid</surname><given-names>CH</given-names></name> <name><surname>Rones</surname><given-names>R</given-names></name> <name><surname>Kalish</surname><given-names>R</given-names></name> <name><surname>Yinh</surname><given-names>J</given-names></name> <name><surname>Goldenberg</surname><given-names>DL</given-names></name> <etal/></person-group>. <article-title>A randomized trial of tai chi for fibromyalgia</article-title>. <source>N Engl J Med</source>. (<year>2010</year>) <volume>363</volume>:<fpage>743</fpage>&#x2013;<lpage>54</lpage>. doi: <pub-id pub-id-type="doi">10.1056/NEJMoa0912611</pub-id>, <pub-id pub-id-type="pmid">20818876</pub-id></mixed-citation></ref>
<ref id="ref14"><label>14.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Birdee</surname><given-names>GS</given-names></name> <name><surname>Wayne</surname><given-names>PM</given-names></name> <name><surname>Davis</surname><given-names>RB</given-names></name> <name><surname>Phillips</surname><given-names>RS</given-names></name> <name><surname>Yeh</surname><given-names>GY</given-names></name></person-group>. <article-title>T'ai chi and Qigong for health: patterns of use in the United States</article-title>. <source>J Alternat Complement Med</source>. (<year>2009</year>) <volume>15</volume>:<fpage>969</fpage>&#x2013;<lpage>73</lpage>. doi: <pub-id pub-id-type="doi">10.1089/acm.2009.0174</pub-id>, <pub-id pub-id-type="pmid">19757974</pub-id></mixed-citation></ref>
<ref id="ref15"><label>15.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pan</surname><given-names>J</given-names></name> <name><surname>Xie</surname><given-names>Z</given-names></name> <name><surname>Ye</surname><given-names>S</given-names></name> <name><surname>Shen</surname><given-names>H</given-names></name> <name><surname>Huang</surname><given-names>Z</given-names></name> <name><surname>Zhang</surname><given-names>X</given-names></name> <etal/></person-group>. <article-title>The effects of tai chi on clinical outcomes and gait biomechanics in knee osteoarthritis patients: a pilot randomized controlled trial</article-title>. <source>Sci Rep</source>. (<year>2025</year>) <volume>15</volume>:<fpage>18495</fpage>. doi: <pub-id pub-id-type="doi">10.1038/s41598-025-03943-3</pub-id>, <pub-id pub-id-type="pmid">40425665</pub-id></mixed-citation></ref>
<ref id="ref16"><label>16.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>X</given-names></name> <name><surname>Chang</surname><given-names>P</given-names></name> <name><surname>Wu</surname><given-names>M</given-names></name> <name><surname>Jiang</surname><given-names>Y</given-names></name> <name><surname>Gao</surname><given-names>Y</given-names></name> <name><surname>Chen</surname><given-names>H</given-names></name> <etal/></person-group>. <article-title>Effect of tai chi vs aerobic exercise on blood pressure in patients with prehypertension: a randomized clinical trial</article-title>. <source>JAMA Netw Open</source>. (<year>2024</year>) <volume>7</volume>:<fpage>e2354937</fpage>. doi: <pub-id pub-id-type="doi">10.1001/jamanetworkopen.2023.54937</pub-id>, <pub-id pub-id-type="pmid">38335001</pub-id></mixed-citation></ref>
<ref id="ref17"><label>17.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhong</surname><given-names>W</given-names></name> <name><surname>Huang</surname><given-names>W</given-names></name> <name><surname>Deng</surname><given-names>H</given-names></name> <name><surname>Qiu</surname><given-names>S</given-names></name> <name><surname>Yang</surname><given-names>Q</given-names></name> <name><surname>Jia</surname><given-names>H</given-names></name></person-group>. <article-title>A randomized controlled trial to assess the efficacy of standardized tai chi in Prefrail older adults with Immunosenescence: design and protocol</article-title>. <source>BMC Complement Med Ther</source>. (<year>2025</year>) <volume>25</volume>:<fpage>1</fpage>. doi: <pub-id pub-id-type="doi">10.1186/s12906-024-04732-7</pub-id>, <pub-id pub-id-type="pmid">39754159</pub-id></mixed-citation></ref>
<ref id="ref18"><label>18.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Metzger</surname><given-names>N</given-names></name></person-group>. <article-title>&#x201C;On a strictly scientific basis.&#x201D; German constitutional medicine establishing itself, 1911&#x2013;1921</article-title>. <source>Med J</source>. (<year>2016</year>) <volume>51</volume>:<fpage>209</fpage>&#x2013;<lpage>45</lpage>.</mixed-citation></ref>
<ref id="ref19"><label>19.</label><mixed-citation publication-type="other"><person-group person-group-type="author"><name><surname>Yu, WJ, Ma, MY, Chen, XM, Min, JY, Li, LR, Zheng, YF</surname><given-names>WJ</given-names></name> <name><surname>Ma</surname><given-names>MY</given-names></name> <name><surname>Chen</surname><given-names>XM</given-names></name> <name><surname>Min</surname><given-names>JY</given-names></name> <name><surname>Li</surname><given-names>LR</given-names></name> <name><surname>Zheng</surname><given-names>YF</given-names></name> <etal/></person-group>. Traditional Chinese medicine and constitutional medicine in China, Japan and Korea: a comparative study. <source>Am J Chin Med</source>. (<year>2017</year>) <volume>45</volume>:<fpage>1</fpage>&#x2013;<lpage>12</lpage>. doi: <pub-id pub-id-type="doi">10.1142/S0192415X1750001X</pub-id></mixed-citation></ref>
<ref id="ref20"><label>20.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Faelli</surname><given-names>C</given-names></name></person-group>. <article-title>Present status of constitutional medicine</article-title>. <source>Med Colon</source>. (<year>1950</year>) <volume>15</volume>:<fpage>441</fpage>&#x2013;<lpage>53</lpage>.</mixed-citation></ref>
<ref id="ref21"><label>21.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Klotz</surname><given-names>H</given-names></name></person-group>. <article-title>Scientific method in general medicine, especially in constitutional medicine</article-title>. <source>La Semaine Hopitaux</source>. (<year>1952</year>) <volume>28</volume>:<fpage>915</fpage>&#x2013;<lpage>20</lpage>.</mixed-citation></ref>
<ref id="ref22"><label>22.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yu</surname><given-names>W</given-names></name> <name><surname>Ma</surname><given-names>M</given-names></name> <name><surname>Chen</surname><given-names>X</given-names></name> <name><surname>Min</surname><given-names>J</given-names></name> <name><surname>Li</surname><given-names>L</given-names></name> <name><surname>Zheng</surname><given-names>Y</given-names></name> <etal/></person-group>. <article-title>Traditional Chinese medicine and constitutional medicine in China, Japan and Korea: a comparative study</article-title>. <source>Am J Chin Med</source>. (<year>2017</year>) <volume>45</volume>:<fpage>1</fpage>&#x2013;<lpage>12</lpage>. doi: <pub-id pub-id-type="doi">10.1142/s0192415x1750001x</pub-id>, <pub-id pub-id-type="pmid">28068838</pub-id></mixed-citation></ref>
<ref id="ref23"><label>23.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yoo</surname><given-names>J</given-names></name> <name><surname>Lee</surname><given-names>E</given-names></name> <name><surname>Kim</surname><given-names>C</given-names></name> <name><surname>Lee</surname><given-names>J</given-names></name> <name><surname>Lixing</surname><given-names>L</given-names></name></person-group>. <article-title>Sasang constitutional medicine and traditional Chinese medicine: a comparative overview</article-title>. <source>eCAM</source>. (<year>2012</year>) <volume>2012</volume>:<fpage>980807</fpage>. doi: <pub-id pub-id-type="doi">10.1155/2012/980807</pub-id>, <pub-id pub-id-type="pmid">21941592</pub-id></mixed-citation></ref>
<ref id="ref24"><label>24.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kim</surname><given-names>JY</given-names></name> <name><surname>Pham</surname><given-names>DD</given-names></name></person-group>. <article-title>Sasang constitutional medicine as a holistic tailored medicine</article-title>. <source>Evid Based Complement Alternat Med</source>. (<year>2009</year>) <volume>6</volume>:<fpage>11</fpage>&#x2013;<lpage>9</lpage>. doi: <pub-id pub-id-type="doi">10.1093/ecam/nep100</pub-id>, <pub-id pub-id-type="pmid">19745007</pub-id></mixed-citation></ref>
<ref id="ref25"><label>25.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Han</surname><given-names>Y</given-names></name> <name><surname>Wang</surname><given-names>Y</given-names></name> <name><surname>Shi</surname><given-names>MY</given-names></name> <name><surname>Liu</surname><given-names>Y</given-names></name> <name><surname>Cheng</surname><given-names>XD</given-names></name> <name><surname>Zhou</surname><given-names>Y</given-names></name></person-group>. <article-title>Integrating traditional Chinese medicine constitutions into insomnia management: findings from a cross-sectional study</article-title>. <source>J Integr Med</source>. (<year>2025</year>) <volume>23</volume>:<fpage>382</fpage>&#x2013;<lpage>9</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.joim.2025.05.004</pub-id>, <pub-id pub-id-type="pmid">40506321</pub-id></mixed-citation></ref>
<ref id="ref26"><label>26.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>C</given-names></name> <name><surname>Zhang</surname><given-names>H</given-names></name> <name><surname>Nie</surname><given-names>X</given-names></name> <name><surname>Ding</surname><given-names>F</given-names></name> <name><surname>Liu</surname><given-names>Q</given-names></name> <name><surname>Hou</surname><given-names>L</given-names></name> <etal/></person-group>. <article-title>Traditional Chinese medicine constitution and sarcopenia: a cross-sectional study</article-title>. <source>Front Public Health</source>. (<year>2024</year>) <volume>12</volume>:<fpage>1368933</fpage>. doi: <pub-id pub-id-type="doi">10.3389/fpubh.2024.1368933</pub-id>, <pub-id pub-id-type="pmid">39114511</pub-id></mixed-citation></ref>
<ref id="ref27"><label>27.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Su</surname><given-names>YC</given-names></name> <name><surname>Chen</surname><given-names>LL</given-names></name> <name><surname>Lin</surname><given-names>JD</given-names></name> <name><surname>Lin</surname><given-names>JS</given-names></name> <name><surname>Huang</surname><given-names>YC</given-names></name> <name><surname>Lai</surname><given-names>JS</given-names></name></person-group>. <article-title>Bcq+: a body constitution questionnaire to assess Yang-Xu. Part I: establishment of a first final version through a Delphi process</article-title>. <source>Forschende Komplementarmedizin</source>. (<year>2008</year>) <volume>15</volume>:<fpage>327</fpage>&#x2013;<lpage>34</lpage>. doi: <pub-id pub-id-type="doi">10.1159/000175938</pub-id></mixed-citation></ref>
<ref id="ref28"><label>28.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lu</surname><given-names>ZJ</given-names></name></person-group>. <article-title>The yellow emperor's internal classic, an ancient medical canon of traditional Chinese medicine</article-title>. <source>J Trad Chin Med</source>. (<year>1985</year>) <volume>5</volume>:<fpage>153</fpage>&#x2013;<lpage>4</lpage>.</mixed-citation></ref>
<ref id="ref29"><label>29.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Diaoyuan</surname><given-names>K</given-names></name> <name><surname>Yongxia</surname><given-names>S</given-names></name></person-group>. <article-title>A brief history of human constitutionology</article-title>. <source>J Trad Chin Med</source>. (<year>2006</year>) <volume>26</volume>:<fpage>230</fpage>&#x2013;<lpage>8</lpage>. <pub-id pub-id-type="pmid">17078460</pub-id></mixed-citation></ref>
<ref id="ref30"><label>30.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Coyle</surname><given-names>M</given-names></name> <name><surname>Smith</surname><given-names>C</given-names></name></person-group>. <article-title>A survey comparing Tcm diagnosis, health status and medical diagnosis in women undergoing assisted reproduction</article-title>. <source>Acupunct Med</source>. (<year>2005</year>) <volume>23</volume>:<fpage>62</fpage>&#x2013;<lpage>9</lpage>. doi: <pub-id pub-id-type="doi">10.1136/aim.23.2.62</pub-id>, <pub-id pub-id-type="pmid">16025786</pub-id></mixed-citation></ref>
<ref id="ref31"><label>31.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yang</surname><given-names>JJ</given-names></name> <name><surname>Wang</surname><given-names>Q</given-names></name> <name><surname>Jiang</surname><given-names>W</given-names></name></person-group>. <article-title>Harmonizing the mind and body: the interrelationship between traditional Chinese medicine body constitution, mental health and sleep quality</article-title>. <source>World J Psychiatry</source>. (<year>2025</year>) <volume>15</volume>:<fpage>103033</fpage>. doi: <pub-id pub-id-type="doi">10.5498/wjp.v15.i3.103033</pub-id>, <pub-id pub-id-type="pmid">40110014</pub-id></mixed-citation></ref>
<ref id="ref32"><label>32.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lin</surname><given-names>J-D</given-names></name> <name><surname>Lin</surname><given-names>J-S</given-names></name> <name><surname>Chen</surname><given-names>L-L</given-names></name> <name><surname>Chang</surname><given-names>C-H</given-names></name> <name><surname>Huang</surname><given-names>Y-C</given-names></name> <name><surname>Su</surname><given-names>Y-C</given-names></name></person-group>. <article-title>Bcqs: a body constitution questionnaire to assess stasis in traditional Chinese medicine</article-title>. <source>Eur J Integr Med</source>. (<year>2012</year>) <volume>4</volume>:<fpage>e379</fpage>&#x2013;<lpage>91</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.eujim.2012.05.001</pub-id></mixed-citation></ref>
<ref id="ref33"><label>33.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>LL</given-names></name> <name><surname>Lin</surname><given-names>JS</given-names></name> <name><surname>Lin</surname><given-names>JD</given-names></name> <name><surname>Chang</surname><given-names>CH</given-names></name> <name><surname>Kuo</surname><given-names>HW</given-names></name> <name><surname>Liang</surname><given-names>WM</given-names></name> <etal/></person-group>. <article-title>Bcq+: a body constitution questionnaire to assess yang-xu. Part II: evaluation of reliability and validity</article-title>. <source>Forschende Komplementarmedizin</source>. (<year>2009</year>) <volume>16</volume>:<fpage>20</fpage>&#x2013;<lpage>7</lpage>. doi: <pub-id pub-id-type="doi">10.1159/000197770</pub-id></mixed-citation></ref>
<ref id="ref34"><label>34.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lin</surname><given-names>JS</given-names></name> <name><surname>Chen</surname><given-names>LL</given-names></name> <name><surname>Lin</surname><given-names>JD</given-names></name> <name><surname>Chang</surname><given-names>CH</given-names></name> <name><surname>Huang</surname><given-names>CH</given-names></name> <name><surname>Mayer</surname><given-names>PK</given-names></name> <etal/></person-group>. <article-title>Bcq-: a body constitution questionnaire to assess yin-Xu. Part II: evaluation of reliability and validity</article-title>. <source>Forschende Komplementarmedizin</source>. (<year>2012</year>) <volume>19</volume>:<fpage>285</fpage>&#x2013;<lpage>92</lpage>. doi: <pub-id pub-id-type="doi">10.1159/000346060</pub-id>, <pub-id pub-id-type="pmid">23343583</pub-id></mixed-citation></ref>
<ref id="ref35"><label>35.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Qu</surname><given-names>X</given-names></name> <name><surname>Xiong</surname><given-names>HZ</given-names></name> <name><surname>Qu</surname><given-names>DQ</given-names></name> <name><surname>Liu</surname><given-names>H</given-names></name> <name><surname>Xu</surname><given-names>XX</given-names></name> <name><surname>Sun</surname><given-names>R</given-names></name> <etal/></person-group>. <article-title>Correlation analysis of traditional Chinese medicine constitution and metabolic indexes in general physical examination people</article-title>. <source>Endocr Metab Immune Disord Drug Targets</source>. (<year>2025</year>) <volume>25</volume>:<fpage>560</fpage>&#x2013;<lpage>8</lpage>. doi: <pub-id pub-id-type="doi">10.2174/0118715303302433240918104124</pub-id>, <pub-id pub-id-type="pmid">39364874</pub-id></mixed-citation></ref>
<ref id="ref36"><label>36.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Clement-Carbonell</surname><given-names>V</given-names></name> <name><surname>Portilla-Tamarit</surname><given-names>I</given-names></name> <name><surname>Rubio-Aparicio</surname><given-names>M</given-names></name> <name><surname>Madrid-Valero</surname><given-names>JJ</given-names></name></person-group>. <article-title>Sleep quality, mental and physical health: a differential relationship</article-title>. <source>Int J Environ Res Public Health</source>. (<year>2021</year>) <volume>18</volume>:1&#x2013;8. doi: <pub-id pub-id-type="doi">10.3390/ijerph18020460</pub-id>, <pub-id pub-id-type="pmid">33435528</pub-id></mixed-citation></ref>
<ref id="ref37"><label>37.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kengne</surname><given-names>AP</given-names></name> <name><surname>Czernichow</surname><given-names>S</given-names></name> <name><surname>Huxley</surname><given-names>R</given-names></name> <name><surname>Grobbee</surname><given-names>D</given-names></name> <name><surname>Woodward</surname><given-names>M</given-names></name> <name><surname>Neal</surname><given-names>B</given-names></name> <etal/></person-group>. <article-title>Blood pressure variables and cardiovascular risk: new findings from advance</article-title>. <source>Hypertension</source>. (<year>2009</year>) <volume>54</volume>:<fpage>399</fpage>&#x2013;<lpage>404</lpage>. doi: <pub-id pub-id-type="doi">10.1161/hypertensionaha.109.133041</pub-id>, <pub-id pub-id-type="pmid">19470869</pub-id></mixed-citation></ref>
<ref id="ref38"><label>38.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Moore</surname><given-names>MN</given-names></name> <name><surname>Climie</surname><given-names>RE</given-names></name> <name><surname>Otahal</surname><given-names>P</given-names></name> <name><surname>Schultz</surname><given-names>MG</given-names></name></person-group>. <article-title>Exercise blood pressure and cardiovascular disease risk: a systematic review and meta-analysis of cross-sectional studies</article-title>. <source>J Hypertens</source>. (<year>2021</year>) <volume>39</volume>:<fpage>2395</fpage>&#x2013;<lpage>402</lpage>. doi: <pub-id pub-id-type="doi">10.1097/hjh.0000000000002962</pub-id>, <pub-id pub-id-type="pmid">34738988</pub-id></mixed-citation></ref>
<ref id="ref39"><label>39.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><collab id="coll8">GBD 2021 Stroke Risk Factor Collaborators</collab></person-group>. <article-title>Global, regional, and national burden of stroke and its risk factors, 1990-2021: a systematic analysis for the global burden of disease study 2021</article-title>. <source>Lancet Neurol</source>. (<year>2024</year>) <volume>23</volume>:<fpage>973</fpage>&#x2013;<lpage>1003</lpage>. doi: <pub-id pub-id-type="doi">10.1016/s1474-4422(24)00369-7</pub-id></mixed-citation></ref>
<ref id="ref40"><label>40.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Park</surname><given-names>HS</given-names></name> <name><surname>Park</surname><given-names>SH</given-names></name> <name><surname>Seong</surname><given-names>Y</given-names></name> <name><surname>Kim</surname><given-names>HJ</given-names></name> <name><surname>Choi</surname><given-names>HY</given-names></name> <name><surname>Park</surname><given-names>HC</given-names></name> <etal/></person-group>. <article-title>Cumulative blood pressure load and incident CKD</article-title>. <source>Am J Kidney Dis</source>. (<year>2024</year>) <volume>84</volume>:<fpage>675</fpage>&#x2013;<lpage>85.e1</lpage>. doi: <pub-id pub-id-type="doi">10.1053/j.ajkd.2024.05.015</pub-id></mixed-citation></ref>
<ref id="ref41"><label>41.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><collab id="coll9">GBD 2021 Tobacco Forecasting Collaborators</collab></person-group>. <article-title>Forecasting the effects of smoking prevalence scenarios on years of life lost and life expectancy from 2022 to 2050: a systematic analysis for the global burden of disease study 2021</article-title>. <source>Lancet Public Health</source>. (<year>2024</year>) <volume>9</volume>:<fpage>e729</fpage>&#x2013;<lpage>44</lpage>. doi: <pub-id pub-id-type="doi">10.1016/s2468-2667(24)00166-x</pub-id>, <pub-id pub-id-type="pmid">39366729</pub-id></mixed-citation></ref>
<ref id="ref42"><label>42.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Addleman</surname><given-names>JS</given-names></name> <name><surname>Lackey</surname><given-names>NS</given-names></name> <name><surname>Tobin</surname><given-names>MA</given-names></name> <name><surname>Lara</surname><given-names>GA</given-names></name> <name><surname>Sinha</surname><given-names>S</given-names></name> <name><surname>Morse</surname><given-names>RM</given-names></name> <etal/></person-group>. <article-title>Heart rate variability applications in medical specialties: a narrative review</article-title>. <source>Appl Psychophysiol Biofeedback</source>. (<year>2025</year>) <volume>50</volume>:<fpage>359</fpage>&#x2013;<lpage>81</lpage>. doi: <pub-id pub-id-type="doi">10.1007/s10484-025-09708-y</pub-id>, <pub-id pub-id-type="pmid">40293647</pub-id></mixed-citation></ref>
<ref id="ref43"><label>43.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chalmers</surname><given-names>JA</given-names></name> <name><surname>Quintana</surname><given-names>DS</given-names></name> <name><surname>Abbott</surname><given-names>MJ</given-names></name> <name><surname>Kemp</surname><given-names>AH</given-names></name></person-group>. <article-title>Anxiety disorders are associated with reduced heart rate variability: a Meta-analysis</article-title>. <source>Front Psych</source>. (<year>2014</year>) <volume>5</volume>:<fpage>80</fpage>. doi: <pub-id pub-id-type="doi">10.3389/fpsyt.2014.00080</pub-id>, <pub-id pub-id-type="pmid">25071612</pub-id></mixed-citation></ref>
<ref id="ref44"><label>44.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lu</surname><given-names>IC</given-names></name> <name><surname>Yen Jean</surname><given-names>MC</given-names></name> <name><surname>Lei</surname><given-names>SM</given-names></name> <name><surname>Cheng</surname><given-names>HH</given-names></name> <name><surname>Wang</surname><given-names>JD</given-names></name></person-group>. <article-title>Bsrs-5 (5-item brief symptom rating scale) scores affect every aspect of quality of life measured by Whoqol-Bref in healthy workers</article-title>. <source>Qual Life Res</source>. (<year>2011</year>) <volume>20</volume>:<fpage>1469</fpage>&#x2013;<lpage>75</lpage>. doi: <pub-id pub-id-type="doi">10.1007/s11136-011-9889-4</pub-id>, <pub-id pub-id-type="pmid">21431460</pub-id></mixed-citation></ref>
<ref id="ref45"><label>45.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tu</surname><given-names>CY</given-names></name> <name><surname>Wu</surname><given-names>CS</given-names></name> <name><surname>Yen</surname><given-names>CM</given-names></name> <name><surname>Chang</surname><given-names>HY</given-names></name> <name><surname>Yu</surname><given-names>CY</given-names></name> <name><surname>Chang</surname><given-names>KC</given-names></name> <etal/></person-group>. <article-title>Profiling social detachment in older people in Taiwan: a cluster analysis</article-title>. <source>Int J Geriatr Psychiatry</source>. (<year>2024</year>) <volume>39</volume>:<fpage>e70027</fpage>. doi: <pub-id pub-id-type="doi">10.1002/gps.70027</pub-id>, <pub-id pub-id-type="pmid">39632283</pub-id></mixed-citation></ref>
<ref id="ref46"><label>46.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>HJ</given-names></name> <name><surname>Lin</surname><given-names>YJ</given-names></name> <name><surname>Wu</surname><given-names>PC</given-names></name> <name><surname>Hsu</surname><given-names>WH</given-names></name> <name><surname>Hu</surname><given-names>WC</given-names></name> <name><surname>Wu</surname><given-names>TN</given-names></name> <etal/></person-group>. <article-title>Study on Yang-Xu using body constitution questionnaire and blood variables in healthy volunteers</article-title>. <source>eCAM</source>. (<year>2016</year>) <volume>2016</volume>:<fpage>9437382</fpage>. doi: <pub-id pub-id-type="doi">10.1155/2016/9437382</pub-id>, <pub-id pub-id-type="pmid">27340421</pub-id></mixed-citation></ref>
<ref id="ref47"><label>47.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>CM</given-names></name> <name><surname>Tsai</surname><given-names>YN</given-names></name> <name><surname>Chen</surname><given-names>CH</given-names></name> <name><surname>Tseng</surname><given-names>YH</given-names></name> <name><surname>Jui-Shan Lin</surname><given-names>S</given-names></name> <name><surname>Su</surname><given-names>YC</given-names></name></person-group>. <article-title>Levonorgestrel intrauterine devices improve body constitution deviations in the perspective of traditional Chinese medicine and quality of life in patients with chronic pelvic pain and heavy menstrual bleeding</article-title>. <source>Taiwan J Obstet Gynecol</source>. (<year>2022</year>) <volume>61</volume>:<fpage>989</fpage>&#x2013;<lpage>94</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.tjog.2021.10.012</pub-id>, <pub-id pub-id-type="pmid">36428003</pub-id></mixed-citation></ref>
<ref id="ref48"><label>48.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>CM</given-names></name> <name><surname>Lin</surname><given-names>SJ</given-names></name> <name><surname>Tsai</surname><given-names>YN</given-names></name> <name><surname>Su</surname><given-names>YC</given-names></name> <name><surname>Chen</surname><given-names>IH</given-names></name> <name><surname>Galeano Machuca</surname><given-names>MP</given-names></name> <etal/></person-group>. <article-title>Association between traditional Chinese medicine body constitution deviation and chronic pelvic pain: a cross-sectional study</article-title>. <source>Taiwan J Obstet Gynecol</source>. (<year>2023</year>) <volume>62</volume>:<fpage>713</fpage>&#x2013;<lpage>8</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.tjog.2023.07.013</pub-id>, <pub-id pub-id-type="pmid">37679000</pub-id></mixed-citation></ref>
<ref id="ref49"><label>49.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lin</surname><given-names>JD</given-names></name> <name><surname>Chen</surname><given-names>LL</given-names></name> <name><surname>Lin</surname><given-names>JS</given-names></name> <name><surname>Chang</surname><given-names>CH</given-names></name> <name><surname>Huang</surname><given-names>YC</given-names></name></person-group>. <article-title>BCQ: a body constitution questionnaire to assess yin-Xu</article-title>. <source>Res Complement Med</source>. (<year>2012</year>) <volume>19</volume>:<fpage>234</fpage>&#x2013;<lpage>41</lpage>. doi: <pub-id pub-id-type="doi">10.1159/000343580</pub-id>, <pub-id pub-id-type="pmid">23128097</pub-id></mixed-citation></ref>
<ref id="ref50"><label>50.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tsai</surname><given-names>CI</given-names></name> <name><surname>Su</surname><given-names>YC</given-names></name> <name><surname>Lin</surname><given-names>SY</given-names></name> <name><surname>Lee</surname><given-names>IT</given-names></name> <name><surname>Lee</surname><given-names>CH</given-names></name> <name><surname>Li</surname><given-names>TC</given-names></name></person-group>. <article-title>Reduced health-related quality of life in body constitutions of yin-Xu, and Yang-Xu, stasis in patients with type 2 diabetes: Taichung diabetic body constitution study</article-title>. <source>eCAM</source>. (<year>2014</year>) <volume>2014</volume>:<fpage>309403</fpage>. doi: <pub-id pub-id-type="doi">10.1155/2014/309403</pub-id>, <pub-id pub-id-type="pmid">25093025</pub-id></mixed-citation></ref>
<ref id="ref51"><label>51.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Huang</surname><given-names>YC</given-names></name> <name><surname>Lin</surname><given-names>CJ</given-names></name> <name><surname>Cheng</surname><given-names>SM</given-names></name> <name><surname>Lin</surname><given-names>CK</given-names></name> <name><surname>Lin</surname><given-names>SJ</given-names></name> <name><surname>Su</surname><given-names>YC</given-names></name></person-group>. <article-title>Using Chinese body constitution concepts and measurable variables for assessing risk of coronary artery disease</article-title>. <source>eCAM</source>. (<year>2019</year>) <volume>2019</volume>:<fpage>8218013</fpage>. doi: <pub-id pub-id-type="doi">10.1155/2019/8218013</pub-id>, <pub-id pub-id-type="pmid">31636687</pub-id></mixed-citation></ref>
<ref id="ref52"><label>52.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Buysse</surname><given-names>DJ</given-names></name> <name><surname>Reynolds</surname><given-names>CF</given-names></name> <name><surname>Monk</surname><given-names>TH</given-names></name> <name><surname>Berman</surname><given-names>SR</given-names></name> <name><surname>Kupfer</surname><given-names>DJ</given-names></name></person-group>. <article-title>The Pittsburgh sleep quality index: a new instrument for psychiatric practice and research</article-title>. <source>Psychiatry Res</source>. (<year>1989</year>) <volume>28</volume>:<fpage>193</fpage>&#x2013;<lpage>213</lpage>. doi: <pub-id pub-id-type="doi">10.1016/0165-1781(89)90047-4</pub-id>, <pub-id pub-id-type="pmid">2748771</pub-id></mixed-citation></ref>
<ref id="ref53"><label>53.</label><mixed-citation publication-type="book"><person-group person-group-type="author"><name><surname>Shahoud</surname><given-names>JS</given-names></name> <name><surname>Sanvictores</surname><given-names>T</given-names></name> <name><surname>Aeddula</surname><given-names>NR</given-names></name></person-group>. <article-title>Physiology, arterial pressure regulation</article-title> In: <source>Statpearls</source>. <publisher-loc>Treasure Island (FL)</publisher-loc>:</mixed-citation></ref>
<ref id="ref54"><label>54.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>Q</given-names></name></person-group>. <article-title>Individualized medicine, health medicine, and constitutional theory in Chinese medicine</article-title>. <source>Front Med</source>. (<year>2012</year>) <volume>6</volume>:<fpage>1</fpage>&#x2013;<lpage>7</lpage>. doi: <pub-id pub-id-type="doi">10.1007/s11684-012-0173-y</pub-id>, <pub-id pub-id-type="pmid">22460443</pub-id></mixed-citation></ref>
<ref id="ref55"><label>55.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sagner</surname><given-names>M</given-names></name> <name><surname>McNeil</surname><given-names>A</given-names></name> <name><surname>Puska</surname><given-names>P</given-names></name> <name><surname>Auffray</surname><given-names>C</given-names></name> <name><surname>Price</surname><given-names>ND</given-names></name> <name><surname>Hood</surname><given-names>L</given-names></name> <etal/></person-group>. <article-title>The P4 health Spectrum - a predictive, preventive, personalized and participatory continuum for promoting Healthspan</article-title>. <source>Prog Cardiovasc Dis</source>. (<year>2017</year>) <volume>59</volume>:<fpage>506</fpage>&#x2013;<lpage>21</lpage>. doi: <pub-id pub-id-type="doi">10.1016/j.pcad.2016.08.002</pub-id>, <pub-id pub-id-type="pmid">27546358</pub-id></mixed-citation></ref>
<ref id="ref56"><label>56.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Qu</surname><given-names>Q</given-names></name> <name><surname>Jiang</surname><given-names>R</given-names></name> <name><surname>Luo</surname><given-names>F</given-names></name> <name><surname>Mou</surname><given-names>S</given-names></name> <name><surname>Zhang</surname><given-names>Z</given-names></name> <name><surname>Zhu</surname><given-names>W</given-names></name></person-group>. <article-title>The correlation between traditional Chinese medicine constitution and primary osteoporosis: a systematic review and Meta-analysis</article-title>. <source>Medicine</source>. (<year>2024</year>) <volume>103</volume>:<fpage>e39560</fpage>. doi: <pub-id pub-id-type="doi">10.1097/md.0000000000039560</pub-id>, <pub-id pub-id-type="pmid">39287293</pub-id></mixed-citation></ref>
<ref id="ref57"><label>57.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Guo</surname><given-names>J</given-names></name> <name><surname>Huang</surname><given-names>X</given-names></name> <name><surname>Dou</surname><given-names>L</given-names></name> <name><surname>Yan</surname><given-names>M</given-names></name> <name><surname>Shen</surname><given-names>T</given-names></name> <name><surname>Tang</surname><given-names>W</given-names></name> <etal/></person-group>. <article-title>Aging and aging-related diseases: from molecular mechanisms to interventions and treatments</article-title>. <source>Signal Transduct Target Ther</source>. (<year>2022</year>) <volume>7</volume>:<fpage>391</fpage>. doi: <pub-id pub-id-type="doi">10.1038/s41392-022-01251-0</pub-id>, <pub-id pub-id-type="pmid">36522308</pub-id></mixed-citation></ref>
<ref id="ref58"><label>58.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chung</surname><given-names>KW</given-names></name> <name><surname>Kim</surname><given-names>DH</given-names></name> <name><surname>Jung</surname><given-names>HJ</given-names></name> <name><surname>Arulkumar</surname><given-names>R</given-names></name> <name><surname>Chung</surname><given-names>HY</given-names></name> <name><surname>Yu</surname><given-names>BP</given-names></name></person-group>. <article-title>Chronic inflammation as an underlying mechanism of ageing and ageing-related diseases</article-title>. <source>Subcell Biochem</source>. (<year>2023</year>) <volume>103</volume>:<fpage>31</fpage>&#x2013;<lpage>44</lpage>. doi: <pub-id pub-id-type="doi">10.1007/978-3-031-26576-1_3</pub-id></mixed-citation></ref>
<ref id="ref59"><label>59.</label><mixed-citation publication-type="other"><person-group person-group-type="author"><collab id="coll10">Organization WH</collab></person-group>. Ageing (<year>2024</year>). Available online at: <ext-link xlink:href="https://www.who.int/health-topics/ageing#tab=tab_1" ext-link-type="uri">https://www.who.int/health-topics/ageing#tab=tab_1</ext-link>.</mixed-citation></ref>
<ref id="ref60"><label>60.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sk&#x00FD;bov&#x00E1;</surname><given-names>D</given-names></name> <name><surname>&#x0160;lachtov&#x00E1;</surname><given-names>H</given-names></name> <name><surname>Tom&#x00E1;&#x0161;kov&#x00E1;</surname><given-names>H</given-names></name> <name><surname>Daleck&#x00E1;</surname><given-names>A</given-names></name> <name><surname>Ma&#x010F;ar</surname><given-names>R</given-names></name></person-group>. <article-title>Risk of chronic diseases limiting longevity and healthy aging by lifestyle and socio-economic factors during the life-course - a narrative review</article-title>. <source>Med Pr</source>. (<year>2021</year>) <volume>72</volume>:<fpage>535</fpage>&#x2013;<lpage>48</lpage>. doi: <pub-id pub-id-type="doi">10.13075/mp.5893.01139</pub-id>, <pub-id pub-id-type="pmid">34664558</pub-id></mixed-citation></ref>
<ref id="ref61"><label>61.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhang</surname><given-names>YP</given-names></name> <name><surname>Hu</surname><given-names>RX</given-names></name> <name><surname>Han</surname><given-names>M</given-names></name> <name><surname>Lai</surname><given-names>BY</given-names></name> <name><surname>Liang</surname><given-names>SB</given-names></name> <name><surname>Chen</surname><given-names>BJ</given-names></name> <etal/></person-group>. <article-title>Evidence base of clinical studies on qi gong: a bibliometric analysis</article-title>. <source>Complement Ther Med</source>. (<year>2020</year>) <volume>50</volume>:<fpage>102392</fpage>. doi: <pub-id pub-id-type="doi">10.1016/j.ctim.2020.102392</pub-id>, <pub-id pub-id-type="pmid">32444061</pub-id></mixed-citation></ref>
</ref-list>
<fn-group>
<fn fn-type="custom" custom-type="edited-by" id="fn0001">
<p>Edited by: <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/660707/overview">Fang Zeng</ext-link>, Chengdu University of Traditional Chinese Medicine, China</p>
</fn>
<fn fn-type="custom" custom-type="reviewed-by" id="fn0002">
<p>Reviewed by: <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/1140265/bio/overview">Lissandra Zanovelo Foga&#x00E7;a</ext-link>, Universidade Federal de S&#x00E3;o Paulo, Brazil</p>
<p><ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/3091890/overview">Dejian Duan</ext-link>, Beijing Sport University, China</p>
</fn>
</fn-group>
</back>
</article>