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<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Public Health</journal-id>
<journal-title>Frontiers in Public Health</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Public Health</abbrev-journal-title>
<issn pub-type="epub">2296-2565</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
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<article-meta>
<article-id pub-id-type="doi">10.3389/fpubh.2025.1631757</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Public Health</subject>
<subj-group>
<subject>Clinical Trial</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Efficacy of ankle Kinesio taping on balance and functional performance in obese female collegiate students: a prospective randomized placebo-controlled trial</article-title>
</title-group>
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<name><surname>Allam</surname> <given-names>Nesma M.</given-names></name>
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<name><surname>Eladl</surname> <given-names>Hadaya Mosaad</given-names></name>
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<name><surname>Elsherbini</surname> <given-names>Dalia Mahmoud Abdelmonem</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
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<name><surname>Abulfaraj</surname> <given-names>Moaz</given-names></name>
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<name><surname>Almadani</surname> <given-names>Moneer E.</given-names></name>
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<name><surname>Megahed Ibrahim</surname> <given-names>Ateya</given-names></name>
<xref ref-type="aff" rid="aff5"><sup>5</sup></xref>
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<name><surname>El-Sherbiny</surname> <given-names>Mohamed</given-names></name>
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<name><surname>Eladl</surname> <given-names>Mohamed A.</given-names></name>
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<name><surname>Nosseir</surname> <given-names>Nermine</given-names></name>
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<name><surname>Jamjoom</surname> <given-names>Reda</given-names></name>
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<aff id="aff1"><sup>1</sup><institution>Department of Physical Therapy and Health Rehabilitation, College of Applied Medical Sciences, Jouf University</institution>, <addr-line>Sakaka</addr-line>, <country>Saudi Arabia</country></aff>
<aff id="aff2"><sup>2</sup><institution>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University</institution>, <addr-line>Sakaka</addr-line>, <country>Saudi Arabia</country></aff>
<aff id="aff3"><sup>3</sup><institution>Department of Surgery, Faculty of Medicine, King Abdulaziz University</institution>, <addr-line>Jeddah</addr-line>, <country>Saudi Arabia</country></aff>
<aff id="aff4"><sup>4</sup><institution>Department of Clinical Medicine, College of Medicine, AlMaarefa University</institution>, <addr-line>Riyadh</addr-line>, <country>Saudi Arabia</country></aff>
<aff id="aff5"><sup>5</sup><institution>College of Nursing, Prince Sattam bin Abdulaziz University</institution>, <addr-line>Al-Kharj</addr-line>, <country>Saudi Arabia</country></aff>
<aff id="aff6"><sup>6</sup><institution>Department of Family and Community Health Nursing, Faculty of Nursing, Port Said University</institution>, <addr-line>Port Said</addr-line>, <country>Egypt</country></aff>
<aff id="aff7"><sup>7</sup><institution>Department of Basic Medical Sciences, College of Medicine, AlMaarefa University</institution>, <addr-line>Diriyah</addr-line>, <country>Saudi Arabia</country></aff>
<aff id="aff8"><sup>8</sup><institution>Department of Basic Medical Sciences, College of Medicine, University of Sharjah</institution>, <addr-line>Sharjah</addr-line>, <country>United Arab Emirates</country></aff>
<aff id="aff9"><sup>9</sup><institution>Department of Biomedical Sciences, College of Medicine, Gulf Medical University</institution>, <addr-line>Ajman</addr-line>, <country>United Arab Emirates</country></aff>
<author-notes>
<fn fn-type="edited-by" id="fn0011"><p>Edited by: Jian Sun, Guangzhou Sport University, China</p></fn>
<fn fn-type="edited-by" id="fn0012"><p>Reviewed by: Naveen Sathyaseelan, Saveetha Medical College and Hospital, India</p><p>Taoli Wang, Shanghai Jiao Tong University School of Medicine, China</p></fn>
<corresp id="c001">&#x002A;Correspondence: Nesma M. Allam, <email>nmallam@ju.edu.sa</email></corresp>
<fn fn-type="other" id="fn0001"><p><sup>&#x2020;</sup>ORCID: Nesma M. Allam, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0002-2478-4474">orcid.org/0000-0002-2478-4474</ext-link></p><p>Hadaya Mosaad Eladl, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0002-4083-7070">orcid.org/0000-0002-4083-7070</ext-link></p>
<p>Dalia Mahmoud Abdelmonem Elsherbini, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0001-5262-6134">orcid.org/0000-0001-5262-6134</ext-link></p>
<p>Moaz Abulfaraj, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0001-6893-537X">orcid.org/0000-0001-6893-537X</ext-link></p>
<p>Moneer E. Almadani, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0009-0000-8582-5455">orcid.org/0009-0000-8582-5455</ext-link></p>
<p>Ateya Megahed Ibrahim, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0001-8192-0017">orcid.org/0000-0001-8192-0017</ext-link></p>
<p>Mohamed El-Sherbiny, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0002-0814-1743">orcid.org/0000-0002-0814-1743</ext-link></p>
<p>Mohamed A. Eladl, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0002-5675-0842">orcid.org/0000-0002-5675-0842</ext-link></p>
<p>Nermine Nosseir, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0009-0008-3445-6125">orcid.org/0009-0008-3445-6125</ext-link></p>
<p>Reda Jamjoom, <ext-link ext-link-type="uri" xlink:href="http://orcid.org/0000-0001-9393-9719">orcid.org/0000-0001-9393-9719</ext-link></p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>11</day>
<month>08</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="collection">
<year>2025</year>
</pub-date>
<volume>13</volume>
<elocation-id>1631757</elocation-id>
<history>
<date date-type="received">
<day>20</day>
<month>05</month>
<year>2025</year>
</date>
<date date-type="accepted">
<day>11</day>
<month>07</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2025 Allam, Eladl, Elsherbini, Abulfaraj, Almadani, Megahed Ibrahim, El-Sherbiny, Eladl, Nosseir and Jamjoom.</copyright-statement>
<copyright-year>2025</copyright-year>
<copyright-holder>Allam, Eladl, Elsherbini, Abulfaraj, Almadani, Megahed Ibrahim, El-Sherbiny, Eladl, Nosseir and Jamjoom</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<sec id="sec1">
<title>Objective</title>
<p>This study aimed to evaluate the efficacy of ankle Kinesio taping (KT) on static and dynamic balance and functional performance in obese female collegiate students.</p>
</sec>
<sec id="sec2">
<title>Design</title>
<p>This study was a prospective, randomized controlled trial.</p>
</sec>
<sec id="sec3">
<title>Setting</title>
<p>The research was conducted in outpatient physical therapy settings.</p>
</sec>
<sec id="sec4">
<title>Participants</title>
<p>A total of 40 female collegiate students, aged 18&#x2013;25&#x202F;years, were randomly assigned to two groups of equal size.</p>
</sec>
<sec id="sec5">
<title>Intervention</title>
<p>Group A (KT group) received KT with 75&#x2013;100% tension, and group B (Placebo KT group) received placebo KT with 0% tension. KT was applied to the dominant leg in both groups for 6&#x202F;weeks.</p>
</sec>
<sec id="sec6">
<title>Outcome measures</title>
<p>The primary outcome was static balance, which was measured using the single-leg stance test (SLST). The secondary outcomes were dynamic balance, assessed using the Biodex Balance System (BBS), and functional performance, which was assessed using the single-leg vertical jump test (SLVJT) and single-leg hop test (SLHT). All outcomes were assessed at baseline and after 6&#x202F;weeks.</p>
</sec>
<sec id="sec7">
<title>Results</title>
<p>The KT group showed a statistically significant increase in SLS, SLVJT, and SLHT (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001), compared to the placebo KT group. In contrast, a statistically significant decrease was observed in the OSI, MLSI, and APSI (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001) in the KT group compared to the control group after a period of 6&#x202F;weeks.</p>
</sec>
<sec id="sec8">
<title>Conclusion</title>
<p>The application of a 6-week KT mechanical correction technique can improve static and dynamic balance and functional performance in obese female university students compared to the placebo group. Including KT in the treatment plan is recommended for obese women with balance and functional performance deficits.</p>
</sec>
<sec id="sec5001">
<title>Clinical trial registration</title>
<p><ext-link xlink:href="https://clinicaltrials.gov/" ext-link-type="uri">ClinicalTrials.gov</ext-link>, identifier NCT06195748.</p>
</sec>
</abstract>
<kwd-group>
<kwd>Kinesio taping</kwd>
<kwd>balance</kwd>
<kwd>postural stability</kwd>
<kwd>functional performance</kwd>
<kwd>obese females</kwd>
</kwd-group>
<counts>
<fig-count count="5"/>
<table-count count="3"/>
<equation-count count="0"/>
<ref-count count="63"/>
<page-count count="11"/>
<word-count count="8231"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Public Health Education and Promotion</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec sec-type="intro" id="sec9">
<title>Introduction</title>
<p>Obesity is defined as a body mass index (BMI) greater than 30&#x202F;kg/m<sup>2</sup> (<xref ref-type="bibr" rid="ref1">1</xref>). It is a complex and chronic disease that affects women and older adults, regardless of their geographic region, social status, or economic status (<xref ref-type="bibr" rid="ref2">2</xref>). Excess body mass may increase the risk of deterioration and injury to musculoskeletal structures due to repeated stress during weight-bearing activities (<xref ref-type="bibr" rid="ref3">3</xref>). Furthermore, during the stance phase, the tibiotalar joints can withstand stress up to five times a person&#x2019;s body weight, and this stress is further intensified in obese individuals (<xref ref-type="bibr" rid="ref4">4</xref>).</p>
<p>Excessive weight is one of the factors that cause ankle sprains, anatomical misalignment, postural fluctuation, reduction in muscle power and reaction time, and relaxation of the ankle joint (<xref ref-type="bibr" rid="ref5">5</xref>). Ankle joint relaxation is influenced by sex, with women experiencing a higher incidence of lateral ankle ligament injury in females compared to men. Furthermore, estrogen produced during the menstrual cycle causes excessive laxity in joints and muscles (<xref ref-type="bibr" rid="ref6">6</xref>). This increased laxity limits their ability to control dynamic motion, which explains the higher risk of musculoskeletal injuries in women (<xref ref-type="bibr" rid="ref7">7</xref>).</p>
<p>Kinesio taping (KT) is a method that involves applying a flexible, adhesive cotton tape to the skin. Kinesio tape is non-allergenic, thin, and lightweight; therefore, it can be perceived as a part of the body. It can be stretched by 140% of its original length and maintained in position for 3&#x2013;5&#x202F;days without causing any negative consequences (<xref ref-type="bibr" rid="ref8">8</xref>). Kinesio taping enhances proprioceptive feedback and stimulates neurological responses and somatosensory function in superficial mechanoreceptors (<xref ref-type="bibr" rid="ref9">9</xref>). It has been proposed that the facilitatory impact and mechanical properties of ankle braces might improve postural control, increase ankle joint performance, and reduce ankle instability (<xref ref-type="bibr" rid="ref10">10</xref>).</p>
<p>Kinesio taping has been shown to enhance balance in basketball players with functional ankle instability (FAI) (<xref ref-type="bibr" rid="ref10">10</xref>, <xref ref-type="bibr" rid="ref11">11</xref>), in healthy subjects (<xref ref-type="bibr" rid="ref12">12</xref>), and in those with multiple sclerosis (<xref ref-type="bibr" rid="ref13">13</xref>). Moreover, it may enhance functional performance in participants with and without ankle injuries (<xref ref-type="bibr" rid="ref14">14</xref>). Limited research has evaluated the efficacy of ankle KT on static and dynamic balance and functional performance in obese female university students. The findings of this study would help clinicians in making decisions about the use of KT in obese adult women when improvement in balance and functional performance is desired.</p>
</sec>
<sec sec-type="materials|methods" id="sec10">
<title>Materials and methods</title>
<sec id="sec11">
<title>Study design</title>
<p>This prospective, randomized, double-blind, placebo-controlled study was conducted at the physical therapy laboratories of the College of Applied Medical Sciences, Jouf University, from January to September 2024. The study protocol adhered to the CONSORT standards for randomized trials of alternative treatments. All procedures were approved by the Research Ethics Committee of Qurayyat Health Affairs (IRB-No. 2023-124). This study was registered at <ext-link xlink:href="https://ClinicalTrials.gov" ext-link-type="uri">ClinicalTrials.gov</ext-link> (No. NCT06195748) in accordance with the principles of the Declaration of Helsinki.</p>
</sec>
<sec id="sec12">
<title>Participants</title>
<p>A total of 40 female university students were recruited based on the following inclusion criteria: aged between 18 and 25&#x202F;years and a BMI of 30&#x2013;39.9&#x202F;kg/m<sup>2</sup>. The exclusion criteria included any orthopedic or neurological injuries in the past 6&#x202F;months, edema of the ankle joint, skin sensitivity to KT, auditory/vestibular disorders that compromised balance, visual problems, participants suffering from diabetes mellitus, open wounds, inability to follow instructions due to personal or cognitive problems, and regular physical training in the last 3&#x202F;months. Participants were equally divided into two groups: Group A (KT group) received KT with 75&#x2013;100% tension, and Group B (Placebo KT group) received KT with 0% tension; both KT techniques were applied to the dominant leg for 6&#x202F;weeks. Before participating in the study, the authors explained the procedures, and each participant provided signed informed consent.</p>
</sec>
<sec id="sec13">
<title>Sample size</title>
<p>The sample size was calculated using G-Power software (version 3.1.9.2; Franz Faul, Universit&#x00E4;t Kiel, Germany) according to the single-leg test with the following parameters: &#x03B1;&#x202F;=&#x202F;0.05, effect size&#x202F;=&#x202F;0.25, and &#x03B2;&#x202F;=&#x202F;0.2. The present analysis had an actual power of 80% (1-&#x03B2;), with each group comprising a minimum of 18 subjects. This original estimate was adjusted to 40 participants in both groups, with a 10% attrition rate (<xref ref-type="bibr" rid="ref12">12</xref>).</p>
</sec>
<sec id="sec14">
<title>Randomization</title>
<p>Forty participants were randomly divided into Group A (KT group, <italic>n</italic>&#x202F;=&#x202F;20) and Group B (placebo KT group, <italic>n</italic>&#x202F;=&#x202F;20). The block randomization program was generated by a computer at <ext-link xlink:href="http://www.randomization.com/" ext-link-type="uri">http://www.randomization.com/</ext-link>. To minimize bias and group variability, participants were randomly allocated to blocks 4, 6, and 8 using a 1:1 allocation ratio. Randomization was conducted by a single author who did not participate in recruitment, data gathering, or treatment. To ensure concealed allocation, randomization codes were consecutively labeled and kept confidential in concealed opaque envelopes. After the baseline assessment, the independent investigator opened the next envelope sequentially to reveal the group assignment and directed the taping procedure accordingly.</p>
</sec>
<sec id="sec15">
<title>Blinding</title>
<p>This study was double-blinded. The participants were blinded to group allocation using a placebo taping method that visually resembled the Kinesio taping intervention but did not apply tension. Both the intervention and placebo tapes were applied to the same anatomical region by a certified physical therapist trained in both techniques. The outcome assessors were blinded to group assignments. All balance and functional performance assessments were conducted by a separate team that was not involved in the intervention procedures and was unaware of the participants&#x2019; group assignment. Group codes were used during data entry and analysis to maintain the blinding of the data analyst until the statistical analysis was completed.</p>
</sec>
<sec id="sec16">
<title>Procedures</title>
<p>A certified orthopedic physical therapist conducted a pre-participation orthopedic ankle examination during the first interview with the participants. Patients with congenital and/or neurological abnormalities that might have affected the experimental data were excluded. Ligamentous stability of the ankle was assessed using a stress test, and cutaneous sensation was also examined. The dominant leg was determined using a ball-kick test. The weight of each participant was assessed twice a week in conjunction with KT application to ensure that no weight loss occurred during the study period. Ten minutes of warm-up techniques in the form of jumping, jogging, squatting, and submaximal kicking were performed (<xref ref-type="bibr" rid="ref15">15</xref>).</p>
</sec>
<sec id="sec17">
<title>Intervention</title>
<sec id="sec18">
<title>Study group</title>
<p>Following the approach suggested by Kase et al. (<xref ref-type="bibr" rid="ref16">16</xref>), waterproof flexible adhesive tape (thickness of 0.5&#x202F;mm and width of 5&#x202F;cm) was applied. The posterosuperior mechanical correction glide technique was applied manually to the lateral malleolus of the dominant leg of the participants. Individuals were asked to maintain a standing position with no footwear in a neutral position on a 30&#x202F;cm high tool. An I-shaped KT (20&#x202F;cm in length) was applied starting at the lateral malleolus and directed toward the tibia (middle 1/3). To enhance sensation and improve motion, a tension of 75&#x2013;100% is proposed, as it provides guidance to the ankle joint into a more functional alignment, and higher tension resists excessive inversion or plantarflexion, which are common injury mechanisms for the ankle (<xref ref-type="bibr" rid="ref16">16</xref>) (<xref ref-type="fig" rid="fig1">Figure 1</xref>). After application, maximum tape adhesion was achieved by the therapist stroking the hand along the length of the wide tape three times (<xref ref-type="bibr" rid="ref12">12</xref>). For consistency, the same procedure was performed by the same practitioner for all participants.</p>
<fig position="float" id="fig1">
<label>Figure 1</label>
<caption>
<p>Kinesio tape application on the dominant leg to group A (KT group). <bold>(A)</bold> Starting application of KT at the lateral malleolus, <bold>(B)</bold> application 75&#x2013;100% tension toward the posterior aspect of the leg, <bold>(C)</bold> continue the tension of KT till the medial aspect of the tibia (middle 1/3).</p>
</caption>
<graphic xlink:href="fpubh-13-1631757-g001.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Three black and white images labeled A, B, and C showing a foot wrapped with kinesiology tape. Image A shows a side view of the foot and ankle. Image B shows a rear view, emphasizing the heel and lower leg. Image C shows a side view of the leg, displaying the tape&#x2019;s placement on the calf.</alt-text>
</graphic>
</fig>
</sec>
<sec id="sec19">
<title>Control group</title>
<p>In the placebo control group, KT was applied in the same manner as in the experimental group, but with a longer tape (28&#x202F;cm) with no tape tension or fibular glide (<xref ref-type="fig" rid="fig2">Figure 2</xref>). Placebo taping helped distinguish between true physiological effects and psychological or perceptual changes (<xref ref-type="bibr" rid="ref12">12</xref>).</p>
<fig position="float" id="fig2">
<label>Figure 2</label>
<caption>
<p>Kinesio tape application on the dominant leg to group B (Placebo KT group). <bold>(A)</bold> Starting application of KT at the lateral malleolus, <bold>(B)</bold> apply 0% tension toward the posterior aspect of leg, <bold>(C)</bold> continue application without tension till the medial aspect of tibia (middle 1/3).</p>
</caption>
<graphic xlink:href="fpubh-13-1631757-g002.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Three-panel image showing an ankle with kinesiology tape applied. Panel A shows tape on the inner side of the foot. Panel B displays the tape extending upward along the calf. Panel C illustrates a profile view of the foot with tape from heel to calf.</alt-text>
</graphic>
</fig>
<p>In both groups (experimental and placebo), the tape was replaced every 4th&#x2013;5th day until the end of the study (<xref ref-type="bibr" rid="ref16">16</xref>). Nevertheless, patients were asked to record any experience of pain or discomfort during the interaction with the physiotherapist.</p>
</sec>
<sec id="sec20">
<title>Outcome measures</title>
<p>The main outcome was static balance, which was evaluated using the single-leg stance test (SLST). The secondary outcome measures were dynamic balance, assessed using the Biodex Balance System (BBS), and functional performance, assessed using the single-leg vertical jump test (SLVJT) and single-leg hop test (SLHT). All tests were separated by one-minute rest periods. All participants were evaluated by a blinded examiner at baseline and after 6 weeks.</p>
</sec>
<sec id="sec21">
<title>Static balance</title>
<p>Static balance was evaluated using the SLST. The participants were instructed to stand barefoot on the dominant lower limb with their eyes closed and their hands folded across their chests. The foot of the non-dominant leg was elevated until it was close to the ankle of the stance leg, but without touching it. A stopwatch was used to measure the duration for which the individual was able to stand on one limb in seconds, starting when the participant lifted the foot off the ground and terminating when the participant (1) uncrossed his arms, (2) moved the non-dominant limb close to or far from the dominant leg, or (3) lowered it to contact the ground to maintain balance. (4) After 45&#x202F;s, or (5) the eyes that had been closed were opened. The average of the three test repetitions was calculated (<xref ref-type="bibr" rid="ref17">17</xref>). The Intraclass Correlation Coefficient (ICC) for this test was 0.998 for closed eyes, indicating excellent intra-rater reliability (<xref ref-type="bibr" rid="ref18">18</xref>).</p>
</sec>
<sec id="sec22">
<title>Biodex Balance System</title>
<p>The Biodex Balance System (Biodex Medical Systems Inc., Shirley, NY, United States) was used to evaluate the postural stability. It showed an intra-rater reliability of (ICC&#x202F;=&#x202F;0.83). Each participant was instructed to stand with their upper limbs on their sides and to maintain balance. With a 10-s break between trials, the overall stability index (OSI), mediolateral stability index (MLSI), and anteroposterior stability index (APSI) were measured (<xref ref-type="bibr" rid="ref19">19</xref>). The first step in the test was to enter the required data and test parameters into the Biodex software. Before the main test, the participants were asked to stand barefoot on both legs, with their eyes open, on a medium level of difficulty (level 5), with a 30-s trial period and a 10-s rest period. They also had to complete one familiarization trial to learn how to keep the platform as horizontal as possible using screen visual feedback, and the platform was released after a 5-s delay from pressing the start key (<xref ref-type="bibr" rid="ref20">20</xref>).</p>
</sec>
<sec id="sec23">
<title>Single-leg vertical jump test</title>
<p>The objective of the SLVJT is to land on the floor with the dominant leg after jumping as high as possible from a single foot. The procedures for the SLVJT included the following: (1) The participants stood unsupported on one leg, close to a wall, tapped their palm on the wall at the highest possible vertical point, and then a reference mark was made on the wall by their index finger. (2) At take-off, the participants jumped as far as they could, tapped their hand on the wall at the highest vertical point, landed on the same lower limb, and marked their position on the wall. (3) Jump displacement was measured in centimeters as the difference between the standing reach height and peak jump height. The participants were instructed to use a chosen countermovement without stepping and to freely swing their arms before jumping. The average of the two best outcomes was calculated using three attempts (<xref ref-type="bibr" rid="ref21">21</xref>). The SLVJ test has high reliability with an ICC of 0.93 (<xref ref-type="bibr" rid="ref22">22</xref>).</p>
</sec>
<sec id="sec24">
<title>Single leg hops test</title>
<p>The aim of the SLHT was to jump as far as possible on one foot and land on the same foot while maintaining balance. The SLHT procedure included the following: (1) All participants placed their big toe on a predefined mark on the floor while standing on their dominant leg. (2) The participants landed on the same extremity as far forward as possible after the hop. (3) The participants maintained their landing for at least 2&#x202F;s. The assessor recorded the horizontal displacement, in centimeters, between the big toe starting point and the heel landing mark using a conventional tape measure. Before jumping, participants were instructed to use a selected countermovement without stepping and to swing their arms freely. The average of the two best performances from the three trials was recorded (<xref ref-type="bibr" rid="ref23">23</xref>). The ICC of the SLHT was 0.93 (<xref ref-type="bibr" rid="ref22">22</xref>).</p>
</sec>
<sec id="sec25">
<title>Statistical analysis</title>
<p>GraphPad Prism version 9 was used to analyze the data, which were displayed as the mean &#x00B1; SD. The difference in demographic characteristics between the KT and placebo KT groups was determined using Student&#x2019;s <italic>t</italic>-test. A two-way repeated-measures ANOVA was used to evaluate the outcome variables between the groups. Qualitative data are presented as percentages. We used the chi-square test for comparison between variables. Partial eta square was used to evaluate the effect size between groups. Statistical significance was set at <italic>p&#x202F;&#x003C;</italic> 0.05. A paired <italic>t</italic>-test was used to evaluate the differences within each group. After standardizing the data, all extremes were eliminated. The relationship between SLVJT, SLHT, and OSI post-intervention in both groups was assessed using a linear regression model with a generalized estimating equation (GEE) adjustment. The normality and homoscedasticity of the variance were statistically evaluated prior to using parametric assumptions. <italic>Post-hoc</italic> power analysis for clinical parameters was conducted using MedCalc Software Ltd. (Version 23.2.3) (<xref ref-type="bibr" rid="ref24">24</xref>) to estimate power for comparison of means between the KT and placebo KT groups post-intervention.</p>
</sec>
</sec>
</sec>
<sec sec-type="results" id="sec26">
<title>Results</title>
<p>Fifty-five participants were enrolled in the study. Fifteen patients were excluded: eight did not meet the inclusion criteria, and seven refused to participate (<xref ref-type="fig" rid="fig3">Figure 3</xref>). All participants completed the intervention and were included in the statistical analyses.</p>
<fig position="float" id="fig3">
<label>Figure 3</label>
<caption>
<p>Flowchart of ankle Kinesio taping clinical study.</p>
</caption>
<graphic xlink:href="fpubh-13-1631757-g003.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Flowchart depicting a study&#x2019;s participant process. Fifty-five assessed for eligibility; fifteen excluded. Forty randomized into two groups of twenty: Kinesio taping and Placebo taping. All received and completed interventions. No loss to follow-up or exclusion from analysis.</alt-text>
</graphic>
</fig>
<sec id="sec27">
<title>Subject characteristics</title>
<p><xref ref-type="table" rid="tab1">Table 1</xref> presents the characteristics of the study and control groups of patients. The mean age, weight, height, BMI, and dominant limb did not differ significantly between the two groups (<italic>p</italic>&#x202F;&#x003E;&#x202F;0.05).</p>
<table-wrap position="float" id="tab1">
<label>Table 1</label>
<caption>
<p>Baseline demographic data of the subjects (<italic>N</italic>&#x202F;=&#x202F;40).</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top" rowspan="2">Characteristics</th>
<th align="center" valign="top" colspan="2">Mean &#x00B1; SD</th>
<th align="center" valign="top" rowspan="2">MD</th>
<th/>
<th align="center" valign="top" rowspan="2"><italic>p</italic></th>
<th align="center" valign="top" colspan="2">95% CI</th>
</tr>
<tr>
<th align="center" valign="top">KT<break/>(<italic>N</italic>&#x202F;=&#x202F;20)</th>
<th align="center" valign="top">Placebo KT<break/>(<italic>N</italic>&#x202F;=&#x202F;20)</th>
<th align="center" valign="top">Effect size (&#x03B7;<sup>2</sup>)</th>
<th align="center" valign="top">Lower</th>
<th align="center" valign="top">Upper</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top">Age (years)</td>
<td align="center" valign="top">21.40&#x202F;&#x00B1;&#x202F;2.03</td>
<td align="center" valign="top">21.23&#x202F;&#x00B1;&#x202F;2.03</td>
<td align="center" valign="top">&#x2212;0.18</td>
<td align="center" valign="top">0.002</td>
<td align="center" valign="top">0.99 (ns)</td>
<td align="center" valign="top">&#x2212;1.48</td>
<td align="center" valign="top">1.13</td>
</tr>
<tr>
<td align="left" valign="top">Weight (Kg)</td>
<td align="center" valign="top">86.83&#x202F;&#x00B1;&#x202F;10.52</td>
<td align="center" valign="top">87.70&#x202F;&#x00B1;&#x202F;7.64</td>
<td align="center" valign="top">0.88</td>
<td align="center" valign="top">0.002</td>
<td align="center" valign="top">0.77 (ns)</td>
<td align="center" valign="top">&#x2212;5.01</td>
<td align="center" valign="top">6.76</td>
</tr>
<tr>
<td align="left" valign="top">Height (cm)</td>
<td align="center" valign="top">160.2&#x202F;&#x00B1;&#x202F;5.67</td>
<td align="center" valign="top">160.5&#x202F;&#x00B1;&#x202F;2.82</td>
<td align="center" valign="top">0.30</td>
<td align="center" valign="top">0.001</td>
<td align="center" valign="top">0.83 (ns)</td>
<td align="center" valign="top">&#x2212;2.57</td>
<td align="center" valign="top">3.17</td>
</tr>
<tr>
<td align="left" valign="top">BMI (kg/m<sup>2</sup>)</td>
<td align="center" valign="top">33.87&#x202F;&#x00B1;&#x202F;3.67</td>
<td align="center" valign="top">34.11&#x202F;&#x00B1;&#x202F;3.32</td>
<td align="center" valign="top">0.24</td>
<td align="center" valign="top">0.001</td>
<td align="center" valign="top">0.83 (ns)</td>
<td align="center" valign="top">&#x2212;2.00</td>
<td align="center" valign="top">2.48</td>
</tr>
<tr>
<td align="left" valign="top">Dominant limb</td>
<td/>
<td/>
<td/>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">Right</td>
<td align="center" valign="top">17 (85%)</td>
<td align="center" valign="top">16 (80%)</td>
<td/>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">Left</td>
<td align="center" valign="top">3 (15%)</td>
<td align="center" valign="top">4 (20%)</td>
<td/>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">&#x03A7;<sup>2</sup> (<italic>p</italic>-value)</td>
<td align="center" valign="top" colspan="2">0.17 (0.68)</td>
<td/>
<td/>
<td/>
<td/>
<td/>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>N, number; BMI, Body mass index, mean &#x00B1; SD; <italic>p</italic>&#x202F;&#x2265;&#x202F;0.05, statistically non-significant (ns); <italic>&#x03A7;</italic><sup>2</sup>, Chi-square test.</p>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec28">
<title>Clinical measures</title>
<p>Post-intervention, there was a significant increase in SLS, SLVJT, and SLHT, while OSI, MLSI, and APSI exhibited a significant reduction in the KT group compared to pre-treatment values (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001). The SLS, SLVJT, SLHT, OSI, MLSI, and APSI scores did not differ significantly in the Placebo KT group. No significant differences were observed between the pre- and post-intervention groups for any of the variables. After the intervention, the mean values of the OSI, MLSI, and APSI in the KT group significantly decreased (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001) compared with those in the two group, whereas the SLS, SLVJT, and SLHT groups showed a significant increase in the KT group compared with the Placebo KT group (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001) (<xref ref-type="table" rid="tab2">Table 2</xref>; <xref ref-type="fig" rid="fig4">Figure 4</xref>). A two-way repeated-measures ANOVA showed significant changes in SLS, SLVJT, SLHT, OSI, MLSI, and APSI between the KT and Placebo KT post-intervention, as evidenced by an effect size (&#x03B7;<sup>2</sup>)&#x202F;&#x003E;&#x202F;0.14. Post-hoc power analysis comparing the KT and placebo KT groups revealed a power of &#x003E; 98% for all clinical measures.</p>
<table-wrap position="float" id="tab2">
<label>Table 2</label>
<caption>
<p>Clinical characteristics of subjects in both groups after 6&#x202F;weeks of intervention (<italic>N</italic>&#x202F;=&#x202F;40).</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top" rowspan="2">Variable</th>
<th align="center" valign="top" colspan="4">KT group</th>
<th align="center" valign="top" colspan="4">Placebo KT group</th>
<th align="center" valign="top" rowspan="2">Group &#x00D7; time interaction (F)<break/><italic>p</italic> value</th>
<th align="center" valign="top" rowspan="2"><italic>P<sup>b</sup></italic> between groups</th>
<th align="center" valign="top" rowspan="2">Effect size (&#x03B7;2)</th>
<th align="center" valign="top">&#x002A;<italic>Post-hoc</italic> power analysis</th>
</tr>
<tr>
<th align="center" valign="top">Pre</th>
<th align="center" valign="top">Post</th>
<th align="center" valign="top">MD (95%CI)</th>
<th align="center" valign="top"><italic>P<sup>a</sup></italic> within group</th>
<th align="center" valign="top">Pre</th>
<th align="center" valign="top">Post</th>
<th align="center" valign="top">MD (95%CI)</th>
<th align="center" valign="top"><italic>P<sup>a</sup></italic> within group</th>
<th align="center" valign="top">(1-&#x03B2; err prob) (%)</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" rowspan="2">SLS (sec)</td>
<td align="center" valign="top" rowspan="2">7.37&#x202F;&#x00B1;&#x202F;5.07</td>
<td align="center" valign="top" rowspan="2">15.07&#x202F;&#x00B1;&#x202F;4.86</td>
<td align="center" valign="top" rowspan="2">7.69 (4.51&#x2013;10.87)</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">8.60&#x202F;&#x00B1;&#x202F;4.63</td>
<td align="center" valign="top" rowspan="2">8.83&#x202F;&#x00B1;&#x202F;4.62</td>
<td align="center" valign="top" rowspan="2">0.23 (&#x2212;2.73&#x2013;3.18)</td>
<td align="center" valign="top" rowspan="2">0.88</td>
<td align="center" valign="top"><italic>F</italic><sub>1, 38</sub> =&#x202F;10.69</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">0.54</td>
<td align="center" valign="top" rowspan="2">0.982 (98.2%)</td>
</tr>
<tr>
<td align="center" valign="top">&#x003C;0.01</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">SLVJT (cm)</td>
<td align="center" valign="top" rowspan="2">9.41&#x202F;&#x00B1;&#x202F;3.92</td>
<td align="center" valign="top" rowspan="2">18.01&#x202F;&#x00B1;&#x202F;4.42</td>
<td align="center" valign="top" rowspan="2">8.60 (5.92&#x2013;11.28)</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">10.19&#x202F;&#x00B1;&#x202F;3.06</td>
<td align="center" valign="top" rowspan="2">10.32&#x202F;&#x00B1;&#x202F;3.09</td>
<td align="center" valign="top" rowspan="2">0.13 (&#x2212;1.83&#x2013;2.10)</td>
<td align="center" valign="top" rowspan="2">0.89</td>
<td align="center" valign="top"><italic>F</italic><sub>1, 38</sub> =&#x202F;22.61</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">0.42</td>
<td align="center" valign="top" rowspan="2">&#x003E;0.999 (&#x003E;99.9%)</td>
</tr>
<tr>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">SLHT (cm)</td>
<td align="center" valign="top" rowspan="2">50.80&#x202F;&#x00B1;&#x202F;11.84</td>
<td align="center" valign="top" rowspan="2">67.51&#x202F;&#x00B1;&#x202F;12.12</td>
<td align="center" valign="top" rowspan="2">16.71 (9.04&#x2013;24.38)</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">51.92&#x202F;&#x00B1;&#x202F;11.46</td>
<td align="center" valign="top" rowspan="2">52.54&#x202F;&#x00B1;&#x202F;11.41</td>
<td align="center" valign="top" rowspan="2">0.61 (&#x2212;6.71&#x2013;7.93)</td>
<td align="center" valign="top" rowspan="2">0.87</td>
<td align="center" valign="top"><italic>F</italic><sub>1, 38</sub> =&#x202F;8.67</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">0.51</td>
<td align="center" valign="top" rowspan="2">0.977 (97.7%)</td>
</tr>
<tr>
<td align="center" valign="top">&#x003C;0.01</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">OSI (&#x00B0;)</td>
<td align="center" valign="top" rowspan="2">2.32&#x202F;&#x00B1;&#x202F;0.91</td>
<td align="center" valign="top" rowspan="2">0.78&#x202F;&#x00B1;&#x202F;0.48</td>
<td align="center" valign="top" rowspan="2">&#x2212;1.54 (&#x2212;2.00 to-1.07)</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">2.18&#x202F;&#x00B1;&#x202F;0.61</td>
<td align="center" valign="top" rowspan="2">2.00&#x202F;&#x00B1;&#x202F;0.61</td>
<td align="center" valign="top" rowspan="2">&#x2212;0.19 (&#x2212;0.58&#x2013;0.21)</td>
<td align="center" valign="top" rowspan="2">0.34</td>
<td align="center" valign="top"><italic>F</italic><sub>1, 38</sub> =&#x202F;30.74</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">0.75</td>
<td align="center" valign="top" rowspan="2">&#x003E;0.999 (&#x003E;99.9%)</td>
</tr>
<tr>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">MLSI (&#x00B0;)</td>
<td align="center" valign="top" rowspan="2">2.21&#x202F;&#x00B1;&#x202F;0.74</td>
<td align="center" valign="top" rowspan="2">0.87&#x202F;&#x00B1;&#x202F;0.42</td>
<td align="center" valign="top" rowspan="2">&#x2212;1.34 (&#x2212;1.73 to &#x2212;0.95)</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">2.13&#x202F;&#x00B1;&#x202F;0.74</td>
<td align="center" valign="top" rowspan="2">2.01&#x202F;&#x00B1;&#x202F;0.76</td>
<td align="center" valign="top" rowspan="2">&#x2212;0.12 (&#x2212;0.59&#x2013;0.36)</td>
<td align="center" valign="top" rowspan="2">0.63</td>
<td align="center" valign="top"><italic>F</italic><sub>1, 38</sub> =&#x202F;33.35</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">0.58</td>
<td align="center" valign="top" rowspan="2">&#x003E;0.999 (&#x003E;99.9%)</td>
</tr>
<tr>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">APSI (&#x00B0;)</td>
<td align="center" valign="top" rowspan="2">2.70&#x202F;&#x00B1;&#x202F;0.62</td>
<td align="center" valign="top" rowspan="2">0.97&#x202F;&#x00B1;&#x202F;0.44</td>
<td align="center" valign="top" rowspan="2">&#x2212;1.73 (&#x2212;2.07 to &#x2212;1.39)</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">2.65&#x202F;&#x00B1;&#x202F;0.78</td>
<td align="center" valign="top" rowspan="2">2.54&#x202F;&#x00B1;&#x202F;0.79</td>
<td align="center" valign="top" rowspan="2">&#x2212;0.11 (&#x2212;0.61&#x2013;0.39)</td>
<td align="center" valign="top" rowspan="2">0.66</td>
<td align="center" valign="top"><italic>F</italic><sub>1, 38</sub> =&#x202F;32.39</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001</td>
<td align="center" valign="top" rowspan="2">0.79</td>
<td align="center" valign="top" rowspan="2">&#x003E;0.999 (&#x003E;99.9%)</td>
</tr>
<tr>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>SLS, Single Leg Stance; SLVT, Single Leg Vertical Jump Test; SLHT, Single Leg Hop Test; OSI, Overall Stability Index; MLSI, Medial-Lateral Stability Index; APSI, Anterior&#x2013;Posterior Stability Index; Values are shown as mean &#x00B1; standard deviation (SD). CI, Confidence Interval; MD, Mean Difference; <italic>P<sup>a</sup></italic>, value within group from paired-samples <italic>t</italic>-test; <italic>P<sup>b</sup></italic>, value post-intervention from independent-samples <italic>t</italic>-test; &#x03B7;<sup>2</sup>, partial eta square; group &#x00D7; time interaction <italic>p</italic> value from two-way repeated measures ANOVA.</p>
<p>&#x002A;<italic>Post-hoc</italic> power analysis (1-&#x03B2; error probability) (%) between the KT and placebo KT groups post-intervention.</p>
</table-wrap-foot>
</table-wrap>
<fig position="float" id="fig4">
<label>Figure 4</label>
<caption>
<p>Interactions between group and time for <bold>(A)</bold> Single Leg Stance (sec) <bold>(B)</bold> Single Leg Vertical Jump Test (cm) <bold>(C)</bold> Single Leg Hop Test (cm) <bold>(D)</bold> Overall Stability Index <bold>(E)</bold> Medio-Lateral Stability Index <bold>(F)</bold> Antero-Posterior Stability Index. Data are expressed as mean &#x00B1; SD. <sup>&#x002A;&#x002A;&#x002A;</sup><italic>p</italic>&#x202F;&#x003C;&#x202F;0.001 within KT group pre <italic>vs.</italic> post-intervention, <sup>###</sup><italic>p</italic>&#x202F;&#x003C;&#x202F;0.001 of KT <italic>vs.</italic> KT placebo group post-intervention, ns: non-significant.</p>
</caption>
<graphic xlink:href="fpubh-13-1631757-g004.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Six line graphs compare the effects of KT and Placebo KT on various assessments: single leg stance, vertical jump, hop test, overall stability, medial-lateral stability, and anterior-posterior stability, pre- and post-intervention. Graphs illustrate that KT shows significant improvement (indicated by asterisks) compared to Placebo KT, especially post-intervention. Performance is measured in seconds, centimeters, and degrees, with arrows showing better or worse outcomes.</alt-text>
</graphic>
</fig>
</sec>
<sec id="sec29">
<title>Correlation between SLS, SLVJT, and SLHT and OSI</title>
<p><xref ref-type="table" rid="tab3">Table 3</xref> shows a weak positive correlation between the SLVJT and OSI tests in the Placebo KT group (<italic>r</italic>&#x202F;=&#x202F;0.18), but a negative correlation in the KT group (<italic>r</italic>&#x202F;=&#x202F;&#x2212;0.06). Linear regression analysis showed no statistically significant differences. The increased SLVJT explained a 0.04% decrease in OSI in the KT group and a 3% increase in the KT placebo group post-intervention (<xref ref-type="fig" rid="fig5">Figure 5A</xref>). There was a weak positive correlation between SLHT and OSI in the KT placebo group (<italic>r</italic>&#x202F;=&#x202F;0.20) but a negative correlation in the KT group (<italic>r</italic>&#x202F;=&#x202F;&#x2212;0.09). Linear regression analysis showed non-significant results. In the KT placebo group, an increase in SLHT was associated with a 4% increase in the OSI. In contrast, an increase in SLHT in the KT group was related to a 0.09% decrease in OSI (<xref ref-type="fig" rid="fig5">Figure 5B</xref>) post-intervention.</p>
<table-wrap position="float" id="tab3">
<label>Table 3</label>
<caption>
<p>Factors associated with the overall stability index in the KT group <italic>vs.</italic> the placebo KT group post-intervention.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top" rowspan="2">Regression statistics</th>
<th align="center" valign="top" colspan="2">Single leg vertical jump test</th>
<th align="center" valign="top" colspan="2">Single leg hop test</th>
</tr>
<tr>
<th align="center" valign="top">KT group</th>
<th align="center" valign="top">Placebo KT group</th>
<th align="center" valign="top">KT group</th>
<th align="center" valign="top">Placebo KT group</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top">Correlation coefficient (<italic>r</italic>)</td>
<td align="center" valign="top">&#x2212;0.06</td>
<td align="center" valign="top">0.18</td>
<td align="center" valign="top">&#x2212;0.09</td>
<td align="center" valign="top">0.20</td>
</tr>
<tr>
<td align="left" valign="top"><italic>R</italic>
<sup>2</sup>
</td>
<td align="center" valign="top">0.004</td>
<td align="center" valign="top">0.03</td>
<td align="center" valign="top">0.009</td>
<td align="center" valign="top">0.04</td>
</tr>
<tr>
<td align="left" valign="top">Adjusted <italic>R</italic><sup>2</sup></td>
<td align="center" valign="top">&#x2212;0.05</td>
<td align="center" valign="top">&#x2212;0.02</td>
<td align="center" valign="top">&#x2212;0.05</td>
<td align="center" valign="top">&#x2212;0.01</td>
</tr>
<tr>
<td align="left" valign="top" colspan="5">Regression parameters</td>
</tr>
<tr>
<td align="left" valign="top">B (95% CI)</td>
<td align="center" valign="top">&#x2212;0.01 (&#x2212;0.06, 0.05)</td>
<td align="center" valign="top">0.03 (&#x2212;0.06 to 0.13)</td>
<td align="center" valign="top">&#x2013;0.004 (&#x2212;0.02, 0.02)</td>
<td align="center" valign="top">0.01 (&#x2212;0.02 to 0.04)</td>
</tr>
<tr>
<td align="left" valign="top">Beta</td>
<td align="center" valign="top">&#x2212;0.06</td>
<td align="center" valign="top">0.18</td>
<td align="center" valign="top">&#x2212;0.09</td>
<td align="center" valign="top">0.20</td>
</tr>
<tr>
<td align="left" valign="top">SE</td>
<td align="center" valign="top">0.03</td>
<td align="center" valign="top">0.05</td>
<td align="center" valign="top">0.009</td>
<td align="center" valign="top">0.01</td>
</tr>
<tr>
<td align="left" valign="top"><italic>t</italic>-value</td>
<td align="center" valign="top">&#x2212;0.26</td>
<td align="center" valign="top">0.76</td>
<td align="center" valign="top">&#x2212;0.40</td>
<td align="center" valign="top">0.86</td>
</tr>
<tr>
<td align="left" valign="top"><italic>P</italic>-value</td>
<td align="center" valign="top">0.80 (ns)</td>
<td align="center" valign="top">0.46 (ns)</td>
<td align="center" valign="top">0.70 (ns)</td>
<td align="center" valign="top">0.40 (ns)</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>r</italic>, Pearson coefficient; <italic>R</italic><sup>2</sup>, R square coefficient of determination; B, unstandardized coefficient estimate; Beta, standardized coefficient estimate; SE, standard error of the coefficient estimate; CI, confidence interval; <italic>p</italic>&#x202F;&#x2264;&#x202F;0.05 is statistically significant; ns, non-significant.</p>
</table-wrap-foot>
</table-wrap>
<fig position="float" id="fig5">
<label>Figure 5</label>
<caption>
<p>Post-intervention linear regression: <bold>(A)</bold> single-leg vertical jump test and overall stability index and <bold>(B)</bold> single-leg hop test and overall stability index.</p>
</caption>
<graphic xlink:href="fpubh-13-1631757-g005.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Two scatter plots compare the overall stability index against two tests: single leg vertical jump (A) and single leg hop (B). In both plots, blue circles and lines represent KT treatment, while red squares and lines represent placebo KT. Trend lines in both plots show different slopes for KT and placebo. The vertical jump test shows minimal correlation for both treatments, with R squared values near zero. The hop test shows slightly stronger correlations. Equations and correlations are provided.</alt-text>
</graphic>
</fig>
</sec>
</sec>
<sec sec-type="discussion" id="sec30">
<title>Discussion</title>
<p>The main results of the current study revealed that ankle KT significantly improved static and dynamic balance and functional performance when compared to placebo KT in obese female university participants (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001). The present study was conducted on female individuals only, as it is suggested that women have a 25% greater chance of sustaining ankle sprains (Grade I), which is the most common injury, than their male counterparts (<xref ref-type="bibr" rid="ref25">25</xref>). Additionally, ankle instability may not be linked to the injury because the ligaments that connect the ankle bones are only microscopically stretched rather than ruptured (<xref ref-type="bibr" rid="ref26">26</xref>). Given that women often have higher laxity in their ankle joints, it is plausible that KT improves stability in women. Males also have more active muscle stiffness than females, which increases their resistance to changes in muscle length, suggesting that males have more stable joints. Thus, it is possible that females with poorer joint stability benefit more from tactile input (<xref ref-type="bibr" rid="ref27">27</xref>).</p>
<p>The main results of the current study showed that ankle KT resulted in a statistically significant increase in SLS and a significant decrease in OSI, MLSI, and APSI compared with the placebo KT group (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001). Because static and dynamic balance are modulated by various mechanisms, they cannot be coupled; hence, they were assessed separately (<xref ref-type="bibr" rid="ref28">28</xref>).</p>
<p>Kinesio taping (KT) is believed to improve balance through several physiological mechanisms. KT primarily enhances proprioceptive feedback by stimulating cutaneous mechanoreceptors in the skin, thereby increasing sensory input to the central nervous system and improving joint position sense, a crucial component of balance control (<xref ref-type="bibr" rid="ref29">29</xref>). Additionally, tape can facilitate or inhibit muscle activity depending on its direction and tension, promoting optimal muscle coordination and stability (<xref ref-type="bibr" rid="ref16">16</xref>). KT also serves as a tactile cue, helping individuals maintain or correct postural alignment, which reduces compensatory movements that can impair balance (<xref ref-type="bibr" rid="ref30">30</xref>). Furthermore, by enhancing joint stability and reducing pain through mechanisms such as gate control theory, KT indirectly supports improved balance by enabling more natural and confident movement patterns (<xref ref-type="bibr" rid="ref31">31</xref>). These combined effects make KT a potentially useful adjunct in balance rehabilitation, particularly in populations with proprioceptive or neuromuscular deficits (<xref ref-type="bibr" rid="ref32">32</xref>).</p>
<p>The findings of the current study are consistent with those of previous studies (<xref ref-type="bibr" rid="ref11">11</xref>, <xref ref-type="bibr" rid="ref33 ref34 ref35 ref36 ref37 ref38">33&#x2013;38</xref>). KT improves acute postural control in healthy athletes and those with chronic ankle instability (CAI) (<xref ref-type="bibr" rid="ref11">11</xref>). Moreover, KT applied to the foot improves dynamic postural stability in healthy athletes (<xref ref-type="bibr" rid="ref34">34</xref>). Static and dynamic balance improved after the application of KT combined with dynamic taping for ankle sprains in patients with CAI (<xref ref-type="bibr" rid="ref35">35</xref>). Moreover, OSI, MLSI, and APSI improved following the combination of KT and exercises for ankle instability in recreational runners (<xref ref-type="bibr" rid="ref36">36</xref>). Additionally, in patients with first-degree ankle sprains, KT improved dynamic postural control, as evaluated using the Star Excursion Balance Test (SEBT), more than no tape and athletic tape (<xref ref-type="bibr" rid="ref33">33</xref>). Furthermore, ankle stability, as well as static and dynamic balance, improved in participants with ankle instability as a result of the mechanical enhancement of the muscles and compression of the joint by flexible taping (<xref ref-type="bibr" rid="ref37">37</xref>). Six weeks of KT combined with balance exercises enhanced the balance and stability scores of female athletic participants (<xref ref-type="bibr" rid="ref38">38</xref>).</p>
<p>KT has an immediate effect on dynamic balance and postural stability, attributed to KT&#x2019;s elastic nature, which may regulate balance by promoting proprioception around the ankle joint (<xref ref-type="bibr" rid="ref39">39</xref>). The potential benefits of KT on dynamic balance were only obvious with prolonged tape application (after 24&#x202F;h) in women (<xref ref-type="bibr" rid="ref40">40</xref>).</p>
<p>In contrast to the present study results, some studies have revealed no beneficial efficacy of KT mechanical correction techniques on dynamic postural control using different taping materials (<xref ref-type="bibr" rid="ref41">41</xref>, <xref ref-type="bibr" rid="ref42">42</xref>). This contradiction may be attributed to the fact that the injuries of individuals with CAI result from a lack of proprioception brought on by central mechanisms or by using different balancing techniques, compensating for employing more proximal (hip) than ankle strategies during static balance (<xref ref-type="bibr" rid="ref41">41</xref>). Additionally, using computerized dynamic posturography, Mulligan ankle taping was found to have no discernible effect on postural and motor control in healthy participants (<xref ref-type="bibr" rid="ref42">42</xref>). Other studies on the immediate effect of KT on balance (<xref ref-type="bibr" rid="ref43 ref44 ref45">43&#x2013;45</xref>) contradicted the results of the present study. KT has a limited ability to improve postural stability in patients with persistent ankle sprains (<xref ref-type="bibr" rid="ref43">43</xref>). This may be because the KT was not sufficiently taut to support the deep sensory receptors found in the tendon organs and muscle spindles. Similarly, there was no discernible variation in balance or functional performance after applying KT to the calf muscle (<xref ref-type="bibr" rid="ref44">44</xref>). These results may be attributed to the different populations, including healthy active individuals, and various KT application techniques and tensions (KT applied with 50% tension on the gastrocnemius muscle starting from the origin and ending at the insertion). Additionally, KT did not improve static or dynamic balance compared to no tape or placebo (<xref ref-type="bibr" rid="ref45">45</xref>). This may be due to the short duration of the study and the different application techniques used in the study. Moreover, taping did not improve postural control during sagittal and frontal plane landing tasks in subjects with CAI (<xref ref-type="bibr" rid="ref46">46</xref>). This contradiction might be due to differences in sample size and measurement tools.</p>
<p>In addition, KT did not improve the sense of stability in athletes with ankle instability (<xref ref-type="bibr" rid="ref47">47</xref>). This difference from the current finding may be related to the different population, sample size, and technique of application, as it was applied as a single strip from the origin to the insertion of the fibularis longus muscle.</p>
<p>For participants with CAI, no significant change in dynamic postural stability was observed after the application of either taping mechanism (lateral subtalar sling or fibular repositioning) (<xref ref-type="bibr" rid="ref41">41</xref>). This contradiction may be due to the small sample size (only 16 participants) and the different application techniques used.</p>
<p>In terms of functional performance, the present study revealed a significant increase in SLVJT and SLHT in the KT group compared with the Placebo KT group (<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001). This notable effect might result from the stimulatory activity of KT on different sensory receptors on the skin, which enhances the capacity of the muscles to contract, thereby increasing the function of the muscles and joints and enhancing muscle power and gait (<xref ref-type="bibr" rid="ref48">48</xref>). KT is clinically helpful in the management of lateral ankle injuries as it improves proprioception, muscular endurance, and activity performance (<xref ref-type="bibr" rid="ref49">49</xref>). It has been proposed that improving the mobility and stability of the ankle and foot enhances the ability to jump and land (<xref ref-type="bibr" rid="ref50">50</xref>).</p>
<p>The conclusions of the current study are in line with prior studies (<xref ref-type="bibr" rid="ref39">39</xref>, <xref ref-type="bibr" rid="ref51 ref52 ref53 ref54">51&#x2013;54</xref>). KT is efficient in improving ankle functional performance in healthy individuals (<xref ref-type="bibr" rid="ref51">51</xref>, <xref ref-type="bibr" rid="ref53">53</xref>, <xref ref-type="bibr" rid="ref54">54</xref>). Furthermore, it may improve the strength and performance of male semi-professional soccer players with and without FAI, as measured using the hop test immediately after its application (<xref ref-type="bibr" rid="ref39">39</xref>, <xref ref-type="bibr" rid="ref52">52</xref>).</p>
<p>In contrast to the current results, previous studies (<xref ref-type="bibr" rid="ref55">55</xref>, <xref ref-type="bibr" rid="ref56">56</xref>) found a non-significant effect of KT on functional performance. In young, healthy individuals, ankle KT does not affect vertical jump height (<xref ref-type="bibr" rid="ref55">55</xref>). This discrepancy may be attributed to variations in application techniques, KT tension, or patient populations. Moreover, KT applied to a healthy population found no improvement or worsening of the hop test (<xref ref-type="bibr" rid="ref56">56</xref>). A possible explanation for this contradiction might be the differing characteristics of the populations, as well as the different treatment durations.</p>
<p>Additionally, in healthy athletes, KT application did not immediately promote functional performance, as evaluated by the highest vertical jump performance and peak jump power in the vertical jump test (<xref ref-type="bibr" rid="ref57">57</xref>). This contradiction might be due to differences in the population, application techniques, and treatment duration.</p>
<p>The effect of taping versus preventive bracing techniques on functional balance and jumping performance of physically active male university students was evaluated, and there were no significant effects between the modalities (<xref ref-type="bibr" rid="ref58">58</xref>), possibly due to differences in measurement tools.</p>
<p>The findings of the current study showed a weak positive correlation between the SLVJT and OSI tests in the Placebo KT group (<italic>r</italic>&#x202F;=&#x202F;0.18), whereas a negative correlation was observed in the KT group (<italic>r</italic>&#x202F;=&#x202F;&#x2212;0.06). In addition, there was a weak positive correlation between SLHT and OSI in the KT placebo group (<italic>r</italic>&#x202F;=&#x202F;0.20), but a negative correlation in the KT group (<italic>r</italic>&#x202F;=&#x202F;&#x2212;0.09). Given that dynamic balance describes the capacity to sustain a steady center of gravity throughout periods of movement, such as hop distance, it seems to be one of the most important physical-functional aspects affecting SLHT values (<xref ref-type="bibr" rid="ref59">59</xref>).</p>
<p>In line with the present study results, KT showed a statistically significant negative correlation with the single-leg Balance Error Scoring System (BESS) score and vertical jumping performance (<italic>r</italic>&#x202F;=&#x202F;&#x2212;0.596) in football players (<xref ref-type="bibr" rid="ref60">60</xref>). Moreover, it has been proven that balance training boosts jump height and that jump training enhances balance performance (<xref ref-type="bibr" rid="ref61">61</xref>). Similarly, balance training has been shown to improve agility and vertical jump scores in physical education students and those who participate in hands-on recreational activities (<xref ref-type="bibr" rid="ref62">62</xref>). Furthermore, it contributes to improved performance in single-leg hop tests in young elite female basketball players (<xref ref-type="bibr" rid="ref59">59</xref>). Additionally, increased dynamic stability enhances one-legged hop-landing biomechanics (<xref ref-type="bibr" rid="ref63">63</xref>).</p>
</sec>
<sec id="sec31">
<title>Implications for rehabilitation</title>
<list list-type="bullet">
<list-item><p>Rehabilitation professionals should implement mechanical correction techniques for ankle KT as an effective strategy to improve balance and functional performance in obese female university students in the future.</p></list-item>
<list-item><p>The mechanical correction technique of ankle KT should be tailored to address the specific needs of young obese females to enhance musculoskeletal health, reduce the risk of falls, and limit ankle injury risk.</p></list-item>
<list-item><p>The mechanical correction technique of ankle KT provides a safe and non-pharmacological adjuvant therapy for enhancing balance and musculoskeletal performance in obese females.</p></list-item>
</list>
</sec>
<sec id="sec32">
<title>Limitations and recommendations</title>
<p>This study had several limitations that warrant consideration. This study included only healthy obese females. Future research should ideally include studies involving both sexes. Furthermore, this study lacked long-term follow-up evaluations, which makes it desirable for future research to conduct long-term follow-up studies to address this limitation.</p>
</sec>
<sec sec-type="conclusions" id="sec33">
<title>Conclusion</title>
<p>Ankle Kinesio taping, using the mechanical correction technique for six weeks, can improve static and dynamic balance, as well as functional performance in obese female university students, making it a beneficial addition to rehabilitation programs targeting balance impairments in this population.</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="sec34">
<title>Data availability statement</title>
<p>The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.</p>
</sec>
<sec sec-type="ethics-statement" id="sec35">
<title>Ethics statement</title>
<p>The studies involving humans were approved by the Institutional Review Board (IRB) of the Research Ethics Committee, Qurayyat Health Affairs, Aljouf (number: 2023-124). The studies were conducted in accordance with the local legislation and institutional requirements. The participants provided their written informed consent to participate in this study.</p>
</sec>
<sec sec-type="author-contributions" id="sec36">
<title>Author contributions</title>
<p>NA: Conceptualization, Supervision, Validation, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing, Funding acquisition. HE: Formal analysis, Investigation, Methodology, Writing &#x2013; review &#x0026; editing, Funding acquisition. DE: Data curation, Formal analysis, Resources, Writing &#x2013; original draft. MA: Methodology, Project administration, Resources, Writing &#x2013; original draft. MEA: Project administration, Supervision, Writing &#x2013; review &#x0026; editing. AM: Investigation, Project administration, Resources, Writing &#x2013; original draft. ME-S: Conceptualization, Methodology, Resources, Writing &#x2013; original draft. ME: Formal analysis, Resources, Supervision, Writing &#x2013; original draft. NN: Data curation, Investigation, Validation, Writing &#x2013; review &#x0026; editing. RJ: Investigation, Validation, Visualization, Writing &#x2013; original draft.</p>
</sec>
<sec sec-type="funding-information" id="sec37">
<title>Funding</title>
<p>The author(s) declare that financial support was received for the research and/or publication of this article. This work was funded by the Deanship of Graduate Studies and Scientific Research at Jouf University under grant No. (DGSSR-2024-01-01012).</p>
</sec>
<sec sec-type="COI-statement" id="sec38">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="ai-statement" id="sec39">
<title>Generative AI statement</title>
<p>The authors declare that no Gen AI was used in the creation of this manuscript.</p>
</sec>
<sec sec-type="disclaimer" id="sec40">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
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