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<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Public Health</journal-id>
<journal-title>Frontiers in Public Health</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Public Health</abbrev-journal-title>
<issn pub-type="epub">2296-2565</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fpubh.2025.1616810</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Public Health</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Effects of a 12-hour shift system on sleep and cardiovascular health of male machine and plant operators &#x2013; a longitudinal study over four years</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Seibt</surname>
<given-names>Reingard</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="corresp" rid="c001"><sup>&#x002A;</sup></xref>
<uri xlink:href="https://loop.frontiersin.org/people/1593949/overview"/>
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<contrib contrib-type="author">
<name>
<surname>Kreuzfeld</surname>
<given-names>Steffi</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<contrib contrib-type="author">
<name>
<surname>Hunger</surname>
<given-names>Bettina</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
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<aff id="aff1"><sup>1</sup><institution>Institute for Occupational, Social and Environmental Medicine of the Rostock University Medical Centre, University of Rostock</institution>, <addr-line>Rostock</addr-line>, <country>Germany</country></aff>
<aff id="aff2"><sup>2</sup><institution>Office of ASD&#x002A;BGN Coordination Berlin, German Social Accident Insurance Institution for the Foodstuffs and Catering Industry (BGN), Government Safety Organization Foods and Restaurants</institution>, <addr-line>Mannheim</addr-line>, <country>Germany</country></aff>
<author-notes>
<fn fn-type="edited-by" id="fn0001">
<p>Edited by: Melissa Ulh&#x00F4;a, Faculdade de Medicina do Vale do A&#x00E7;o (UNIVACO), Brazil</p>
</fn>
<fn fn-type="edited-by" id="fn0002">
<p>Reviewed by: Subhashis Sahu, University of Kalyani, India</p>
<p>Dimitrios Papadopoulos, 401 General Military Hospital of Athens, Greece</p>
</fn>
<corresp id="c001">&#x002A;Correspondence: Reingard Seibt, <email>Ringard.seibt@uni-rostock.de</email></corresp>
</author-notes>
<pub-date pub-type="epub">
<day>28</day>
<month>08</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="collection">
<year>2025</year>
</pub-date>
<volume>13</volume>
<elocation-id>1616810</elocation-id>
<history>
<date date-type="received">
<day>23</day>
<month>04</month>
<year>2025</year>
</date>
<date date-type="accepted">
<day>31</day>
<month>07</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2025 Seibt, Kreuzfeld and Hunger.</copyright-statement>
<copyright-year>2025</copyright-year>
<copyright-holder>Seibt, Kreuzfeld and Hunger</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<sec id="sec1">
<title>Background</title>
<p>Data on the risks and effects of shift systems involving night work are inconsistent. In particular, there is a lack of longitudinal studies on the impact of 12-h shift systems on indicators of sleep, cardiovascular health and work-life balance. Therefore, this study compared machine and plant operators (MPO) who worked in a rotating 12-h shift system or only during the day, both at baseline (T1) and at follow-up 4&#x202F;years later (T5).</p>
</sec>
<sec id="sec2">
<title>Methods</title>
<p>Data were collected annually and included a questionnaire on shift work and sleep as well as a cardiovascular screening programme. The sample for analysis consisted of 45 shift (SW) and 30&#x202F;day workers (DW) (mean age T1: 40&#x202F;years). Sleep behaviour was examined by sleep quality and quantity (PSQI score), cardiovascular health by blood pressure, body mass index (BMI), blood lipids, glycosylated haemoglobin (HbA1c) and PROCAM score. Work-life balance was assessed on the basis of life satisfaction and impairments. Analyses of covariance with repeated measures were used to determine longitudinal changes in the indicators between T1 and T5.</p>
</sec>
<sec id="sec3">
<title>Results</title>
<p>At T1, SW showed significantly poorer sleep quality (<italic>d</italic>&#x202F;=&#x202F;0.58) and shorter sleep duration (<italic>M</italic>&#x202F;=&#x202F;366&#x202F;min vs. 438&#x202F;min, <italic>d</italic>&#x202F;=&#x202F;1.38) compared to DW. These effects increased significantly in SW only after night shifts at T5 (<italic>M</italic>&#x202F;=&#x202F;5.1 pts, <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.13, sleep duration: <italic>M</italic>&#x202F;=&#x202F;318&#x202F;min). At T1, SW differed from DW only by a significantly higher blood pressure (<italic>d</italic>&#x202F;=&#x202F;0.60/0.49), BMI (<italic>d</italic>&#x202F;=&#x202F;0.68) and PROCAM score in trend (<italic>p</italic>&#x202F;=&#x202F;0.122). Lipids and HbA1c were comparable between the two groups. The means of the PROCAM score were in the low-moderate range, predicting a risk of heart attack &#x003C;10% for 87% of the MPOs. At T5, the group differences for cardiovascular health from T1 were confirmed. SW achieved significantly higher satisfaction at T5 (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.22); it corresponded to that of DW. Both groups reported significantly fewer impairments at T5 (<italic>d</italic>&#x202F;=&#x202F;0.68/0.58).</p>
</sec>
<sec id="sec4">
<title>Conclusion</title>
<p>At T5, the 12-h shift system demonstrably changed sleep behaviour but not cardiovascular health. Sleep deficits could not be compensated. The 12-h shift system seems to offer advantages for work-life balance.</p>
</sec>
</abstract>
<kwd-group>
<kwd>shift work</kwd>
<kwd>day work</kwd>
<kwd>longitudinal section</kwd>
<kwd>sleep</kwd>
<kwd>health</kwd>
<kwd>work-life balance</kwd>
</kwd-group>
<counts>
<fig-count count="3"/>
<table-count count="5"/>
<equation-count count="0"/>
<ref-count count="89"/>
<page-count count="20"/>
<word-count count="15985"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Occupational Health and Safety</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec sec-type="intro" id="sec5">
<label>1</label>
<title>Introduction</title>
<p>The current labour market continues to be characterised by traditional demands on shift systems with night and weekend work. In certain sectors of the economy, shift work is essential to maintain production processes. In 2020, 13% of men in Germany worked night shifts and 15% worked rotating shifts (<xref ref-type="bibr" rid="ref1">1</xref>).</p>
<p>Shift and night work are defined differently internationally, and studies often use the terms imprecisely (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref3">3</xref>). The different use of the term in the primary studies of the meta-analysis by Ijaz et al. (<xref ref-type="bibr" rid="ref4">4</xref>) prevents a comprehensive assessment of the effects of shift and night work on sleep, health or work-life balance. In Germany, night work is regulated by &#x00A7; 2 of the Working Hours Act (ArbZG) (<xref ref-type="bibr" rid="ref5">5</xref>). It covers the period from 23:00&#x202F;h to 06:00&#x202F;h and applies to any work that extends more than 2&#x202F;h into the night. In addition, in Germany the eight-hour day also applies to shift workers. The German Working Time Act (&#x00A7; 7, &#x00A7; 15, para. 1) only allows a continuous 12-h shift system under certain conditions and with additional regulations.</p>
<p>Although there are concerns about such shift systems for reasons of productivity and performance, there is an interest in 12-h shifts from the perspective of both companies and shift workers themselves (<xref ref-type="bibr" rid="ref6">6</xref>). Employers expect benefits for operational processes and employees expect more free time (<xref ref-type="bibr" rid="ref7">7</xref>, <xref ref-type="bibr" rid="ref8">8</xref>). Overall, the data on the consequences of shift and night work is extensive but inconsistent, which is not least due to the lack of a standardised definition of shift and night work (<xref ref-type="bibr" rid="ref3">3</xref>, <xref ref-type="bibr" rid="ref9">9</xref>) and the large number of different shift systems (<xref ref-type="bibr" rid="ref10">10</xref>).</p>
<p>Obtaining enough high-quality sleep is essential for good health. However, night work leads to a physiological desynchronization of bodily functions (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref11 ref12 ref13">11&#x2013;13</xref>). The change in sleep&#x2013;wake rhythm not only causes additional burden but is also associated with a variety of impairments, most commonly sleep deficits and sleep disorders (<xref ref-type="bibr" rid="ref8">8</xref>, <xref ref-type="bibr" rid="ref12 ref13 ref14 ref15 ref16 ref17">12&#x2013;17</xref>). This leads to sleep-related mood disorders and a lack of recovery (e.g., daytime sleepiness, irritability, concentration problems) (<xref ref-type="bibr" rid="ref14">14</xref>, <xref ref-type="bibr" rid="ref18">18</xref>), which increase the risk of errors and work-related accidents (<xref ref-type="bibr" rid="ref14">14</xref>, <xref ref-type="bibr" rid="ref16">16</xref>, <xref ref-type="bibr" rid="ref18">18</xref>). It has been observed particularly in occupations with a high mental workload after more than 8&#x202F;h of work (<xref ref-type="bibr" rid="ref6">6</xref>, <xref ref-type="bibr" rid="ref18">18</xref>).</p>
<p>Numerous studies have associated night and rotating shift work with an increased risk of cardiovascular disease (<xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref16">16</xref>, <xref ref-type="bibr" rid="ref19">19</xref>, <xref ref-type="bibr" rid="ref20">20</xref>) and metabolic diseases (<xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref16">16</xref>, <xref ref-type="bibr" rid="ref20 ref21 ref22">20&#x2013;22</xref>). The elevated risk of cardiovascular disease (<xref ref-type="bibr" rid="ref15">15</xref>, <xref ref-type="bibr" rid="ref23">23</xref>) and type II diabetes (<xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref15">15</xref>, <xref ref-type="bibr" rid="ref22">22</xref>, <xref ref-type="bibr" rid="ref23">23</xref>) has also been derived from meta-analyses and multi-cohort studies for long working hours. However, other reviews and studies have not provided convincing evidence of a link between shift work and cardiovascular disease (<xref ref-type="bibr" rid="ref24 ref25 ref26">24&#x2013;26</xref>) or type II diabetes (<xref ref-type="bibr" rid="ref21">21</xref>). The longitudinal study (five-year follow-up) by Rossnagel et al. (<xref ref-type="bibr" rid="ref27">27</xref>) also found no significant association between long working hours and cardiovascular disease or type II diabetes.</p>
<p>The contradictory data also applies to cardiovascular risk factors. While a number of studies (<xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref11">11</xref>, <xref ref-type="bibr" rid="ref28 ref29 ref30 ref31">28&#x2013;31</xref>) and the systematic review by Esquirol et al. (<xref ref-type="bibr" rid="ref2">2</xref>) found an association between shift and night work and hypertension, longitudinal studies by Morikawa et al. (<xref ref-type="bibr" rid="ref32">32</xref>) or Akbari et al. (<xref ref-type="bibr" rid="ref28">28</xref>) or the cohort study by Hublin et al. (<xref ref-type="bibr" rid="ref24">24</xref>) did not confirm this correlation.</p>
<p>Night work in particular has been associated with an increased risk of being overweight and obesity in several systematic reviews (<xref ref-type="bibr" rid="ref33 ref34 ref35">33&#x2013;35</xref>). In contrast, no such association was identified in the cohort study by Akbari et al. (<xref ref-type="bibr" rid="ref28">28</xref>) or the meta-analysis by Saulle et al. (<xref ref-type="bibr" rid="ref36">36</xref>). Other studies and the review by Esquirol et al. (<xref ref-type="bibr" rid="ref2">2</xref>) have shown that shift and night workers have raised lipid levels (total cholesterol, low-density lipoprotein and high-density lipoprotein cholesterol, triglycerides) (<xref ref-type="bibr" rid="ref31">31</xref>, <xref ref-type="bibr" rid="ref37">37</xref>) and glucose levels (fast blood sugar, fasting plasma glucose) (<xref ref-type="bibr" rid="ref38">38</xref>), while in the studies by Morikawa et al. (<xref ref-type="bibr" rid="ref32">32</xref>), Akbari et al. (<xref ref-type="bibr" rid="ref28">28</xref>) or Dong et al. (<xref ref-type="bibr" rid="ref30">30</xref>), indicators of lipid and glucose metabolism did not differ between shift and day workers.</p>
<p>It has also been observed that long working hours, shift and night work lead to changes in lifestyle and health behaviour (<xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref12">12</xref>, <xref ref-type="bibr" rid="ref39">39</xref>). For example, it has been found that shift workers have unfavourable eating habits (<xref ref-type="bibr" rid="ref22">22</xref>, <xref ref-type="bibr" rid="ref25">25</xref>), smoke more often (<xref ref-type="bibr" rid="ref22">22</xref>, <xref ref-type="bibr" rid="ref25">25</xref>, <xref ref-type="bibr" rid="ref35">35</xref>) and are less physically active (<xref ref-type="bibr" rid="ref22">22</xref>, <xref ref-type="bibr" rid="ref36">36</xref>, <xref ref-type="bibr" rid="ref40">40</xref>) compared to day workers. Other studies have found no evidence between shift work and physical inactivity (<xref ref-type="bibr" rid="ref41">41</xref>, <xref ref-type="bibr" rid="ref42">42</xref>) or smoking (<xref ref-type="bibr" rid="ref32">32</xref>).</p>
<p>Another well-known consequence of shift and night work is social desynchronization, which manifests in restrictions on family and social life (<xref ref-type="bibr" rid="ref7">7</xref>, <xref ref-type="bibr" rid="ref25">25</xref>, <xref ref-type="bibr" rid="ref43">43</xref>, <xref ref-type="bibr" rid="ref44">44</xref>) and less participation in social activities. Night and weekend shifts, in particular, are at odds with the desire for evenings, weekends and holidays off (<xref ref-type="bibr" rid="ref7">7</xref>). However, the results on the impact on work-life balance have also been found to be contradictory (<xref ref-type="bibr" rid="ref7">7</xref>), with the duration and organisation of working time, as well as the opportunities for employee participation, moderating the result (<xref ref-type="bibr" rid="ref10">10</xref>). Although long working hours appeared to make it more difficult to reconcile work and private life (<xref ref-type="bibr" rid="ref45">45</xref>), a better work-life balance was confirmed for the 12-h shift system (<xref ref-type="bibr" rid="ref46">46</xref>). Loudoun (<xref ref-type="bibr" rid="ref47">47</xref>), on the other hand, found no difference in work-life balance for an eight-hour or 12-h shift system.</p>
<p>To summarise, there is a lack of meaningful longitudinal studies that address the influence of 12-h shift systems on indicators of sleep, cardiovascular health and work-life balance. In addition, the findings are inconsistent due to the heterogeneity of the methodology (e.g., definition of shift work, design, population, data collection, adjustment for potential covariates). Furthermore, the occupational group of machine and plant operators (MPO) is clearly underrepresented in the literature.</p>
<p>MPOs are primarily found in industrial production sectors (including the food industry). They are involved in the entire production process and must guarantee smooth production. Their range of tasks includes setting up, commissioning, operating and maintaining machines and production facilities, selecting and applying mechanical and manual production techniques, controlling the flow of materials and performing quality control and assurance tasks (<xref ref-type="bibr" rid="ref48">48</xref>). In addition, there is coordination with upstream and downstream production levels and the documentation of production data.</p>
<p>For technological and economic reasons, MPOs requires a high proportion of night shift work, although two-shift operation is more common on weekdays in the food and beverage industry. Although a 12-h shift system increases the degree of utilisation of plant capacities, higher labour costs are incurred.</p>
<p>One of the primary stress factors for MPOs is the intense, sustained and concentrated attention required to capture, process and implement relevant process information. This can lead to changes in performance over the course of the day, with a particular drop in performance at night (<xref ref-type="bibr" rid="ref49">49</xref>). Since the operating and control processes are mainly carried out while standing, a solid physical fitness is required. Good stress management is also essential, as maintenance and repair work often has to be carried out under time pressure (<xref ref-type="bibr" rid="ref50">50</xref>).</p>
<p>The aim of the study was therefore to map the development of indicators of sleep, cardiovascular health and work-life balance after a period of 4&#x202F;years (follow-up measurement T5) in MPOs working in a rotating 65-h shift system or exclusively during the day (8&#x202F;h) in comparison to the baseline measurement (T1). It was hypothetically assumed that rotating 12-h shift work over a period of 4&#x202F;years would have a negative impact on sleep, cardiovascular health and work-life balance under comparable baseline conditions for the two groups.</p>
</sec>
<sec sec-type="methods" id="sec6">
<label>2</label>
<title>Methods</title>
<p>This study was a longitudinal study with a baseline measurement (T1) and follow-up after 4&#x202F;years (T5). The data was collected annually from 2011 (first measurement point, T1) to 2016 (fifth measurement point, T5) at a company in the food industry in Saxony-Anhalt (&#x003E;400 examinations). The 12-h shift system was introduced at this company in November 2009. Interim results (T2-T4) are not reported here since they do not provide any additional insights.</p>
<p>A total of 108 employees took part in the study, of whom 75 datasets from male MPOs were available which met the data quality requirements, i.e., 45 shift workers and 30&#x202F;day workers (working regular daytime hours) took part in both the baseline and the follow-up measurement after 4&#x202F;years; they form the data basis of this study. Since there were only four female MPOs in the baseline measurement, no women were included in the sample.</p>
<p>The examinations were carried out by THUMEDI Pr&#x00E4;ventionsmanagement GmbH as part of the occupational health programme for shift workers. The examinations were carried out in the company&#x2019;s medical centres, with each participant being examined for 30&#x202F;min. All participants took part in the examinations voluntarily. Before the start of the examination, all employees received a letter with information on data protection, the conduct of the examination and data analysis. The anonymity of the data was guaranteed by means of a personal code.</p>
<sec id="sec7">
<label>2.1</label>
<title>Research methods</title>
<p>The screening concept was developed by THUMEDI Pr&#x00E4;ventionsmanagement GmbH and the Psychophysiological Diagnostics Department of the Faculty of Medicine at the Technical University of Dresden (<xref ref-type="bibr" rid="ref51">51</xref>) and consisted of a modified shift work questionnaire (<xref ref-type="bibr" rid="ref52">52</xref>), other standardised survey instruments, additional questions and a cardiovascular screening programme. The shift work questionnaire was completed in advance of the examination and brought to the occupational health screening programme. This made it possible to check the completeness of the data on site; inaccuracies could be corrected and missing information completed. Internal consistencies of the scales in the questionnaires were analysed using Cronbach&#x2019;s alpha and evaluated according to Blanz (<xref ref-type="bibr" rid="ref53">53</xref>).</p>
<sec id="sec8">
<label>2.1.1</label>
<title>Shift work questionnaire</title>
<p>The Shift Work Questionnaire (SWQ) was developed based on the international standard Shiftwork Index [SSI, (<xref ref-type="bibr" rid="ref54">54</xref>)] and reduced to reflect the specific characteristics of the MPO job profile (<xref ref-type="bibr" rid="ref52">52</xref>). The SSI includes the following six sections, each focusing on a specific aspect of shift work: 1. general biographical information, 2. sleep and fatigue, 3. health and well-being, 4. social and domestic situation, 5. coping strategies, 6. chronotype and personality. A comprehensive description can be found in Barton et al. (<xref ref-type="bibr" rid="ref54">54</xref>). In this study, selected questions from the SSI were used to examine the family situation [e.g., gender, age, marital status, children in the household (yes/no, number)] and shift-related workload (e.g., job title, years in shift work, weekly working hours, shift system, shift duration) (section 1) as well as health factors [e.g., complaints, illnesses or diagnoses, medication) (section 3) and aspects of work-life balance (section 4)].</p>
<p>The questions on health behaviour (e.g., smoking status, alcohol consumption, sporting activity) were developed in-house. For smoking, respondents were asked whether they are or were smokers (<italic>yes/no</italic>) and the average number of cigarettes consumed per day was recorded. Former smokers were categorised as non-smokers. Questions on alcohol consumption related to the frequency (<italic>not at all, occasionally, regularly</italic>) and amount of alcohol consumed (converted into g/day). Sporting activity was determined on the basis of frequency (<italic>not at all, occasionally, regularly/week</italic>), the amount of time/week and the type of sport practised.</p>
<p>The work-life balance was determined on the basis of two scales: satisfaction with available time (satisfaction scale) and impairment of work and private life (impairment scale). The questions were based on aspects known from the literature on the social consequences of shift work. A total of 13 items were asked, with 8 items on the satisfaction scale and 5 items on the impairment scale. The items for the satisfaction scale were assessed using a three-point response scale (<italic>1&#x202F;=&#x202F;yes, 2&#x202F;=&#x202F;partly, 3&#x202F;=&#x202F;no</italic>), while the items on impairment (work&#x2013;family conflict or family&#x2013;work conflict) were assessed on a five-point response scale (<italic>1&#x202F;=&#x202F;always, 2&#x202F;=&#x202F;frequently, 3&#x202F;=&#x202F;occasionally, 4&#x202F;=&#x202F;rarely, 5&#x202F;=&#x202F;never</italic>), with scales 1 and 2 (<italic>frequently</italic>) and 4 and 5 (<italic>never</italic>) being summarised for the presentation of results. The satisfaction score (range: 8&#x2013;24 pts) and the impairment score (range: 5&#x2013;25 pts) were calculated by adding the respective item details. On the satisfaction scale, a low score indicated a high level of satisfaction with the free time available; on the impairment scale, a low score indicated frequent impairment of work and private life. The Cronbach&#x2019;s alpha for the satisfaction and impairment scales was 0.92 and 0.73 respectively, which according to Blanz (<xref ref-type="bibr" rid="ref53">53</xref>) corresponds to excellent and acceptable quality.</p>
</sec>
<sec id="sec9">
<label>2.1.2</label>
<title>Pittsburgh sleep quality index</title>
<p>The Pittsburgh Sleep Quality Index [PSQI, (<xref ref-type="bibr" rid="ref55">55</xref>)] was used to measure sleep quality and quantity in the German translation by Riemann and Backhaus (<xref ref-type="bibr" rid="ref56">56</xref>), adapted for shift workers (<xref ref-type="bibr" rid="ref52">52</xref>). It consisted of 19 items, which were summarised into the following seven components: sleep quality, usual sleep times, latency to fall asleep and duration of sleep (sleep quantity), sleep disturbances, use of sleep medication, daytime sleepiness. Each component covered the value range from zero to three pts and was surveyed retrospectively for the last 4&#x202F;weeks. The PSQI score results from the summation of the seven component scores and can vary between zero and 21 pts, with a high score corresponding to reduced sleep quality. A cut-off value of five pts allows differentiation between good and poor sleepers, and a cut-off value of 11 pts or more is assumed to indicate chronic sleep disorders (<xref ref-type="bibr" rid="ref55">55</xref>). For shift workers, the items on sleeping and waking times as well as sleep duration in the morning and night shifts were differentiated and the PSQI score was determined for both shifts.</p>
<p>The PSQI has a high or acceptable reliability (<xref ref-type="bibr" rid="ref57">57</xref>): Cronbach&#x2019;s <italic>&#x03B1;</italic> were 0.85 and 0.75. For the present data, Cronbach&#x2019;s alpha was 0.73 and is therefore an acceptable indicator of internal consistency (<xref ref-type="bibr" rid="ref53">53</xref>).</p>
</sec>
<sec id="sec10">
<label>2.1.3</label>
<title>Morningness-Eveningness Questionnaire</title>
<p>To determine the individual chronotype, the validated German version of the Morningness-Eveningness Questionnaire [DMEQ, (<xref ref-type="bibr" rid="ref58">58</xref>)] was used, which was originally developed as an English version by Horne and Ostberg (<xref ref-type="bibr" rid="ref59">59</xref>).</p>
<p>The D-MEQ consists of 19 items with different Likert scales and records temporal preferences and habits of the sleep&#x2013;wake cycle (e.g., preferred bed and getting-up/rising times), performance and subjective well-being. The answers were each given a point value and added up to a total score ranging from 16 to 86 points. Based on this, the following chronotypes were differentiated (<xref ref-type="bibr" rid="ref58">58</xref>): definite morning type (70&#x2013;86 pts), moderate morning type (59&#x2013;69 pts), neutral type (42&#x2013;58 pts), moderate evening type (31&#x2013;41 pts), definite evening type (16&#x2013;30 pts). Thus, a higher score indicates a preference for an earlier arising time, an earlier time for high performance, and an earlier finish time.</p>
<p>The retest reliability of the D-MEQ was reported as rtt&#x202F;=&#x202F;0.96 (<xref ref-type="bibr" rid="ref58">58</xref>), which can be classified as excellent or reliable (<xref ref-type="bibr" rid="ref53">53</xref>). The internal consistency of the D-MEQ varies between 0.84 and 0.87 for Cronbach&#x2019;s alpha (<xref ref-type="bibr" rid="ref60">60</xref>), which is considered high (<xref ref-type="bibr" rid="ref53">53</xref>). In the present study, a Cronbach&#x2019;s alpha of 0.82 was determined for the D-MEQ, which is in the good range (<xref ref-type="bibr" rid="ref53">53</xref>).</p>
</sec>
<sec id="sec11">
<label>2.1.4</label>
<title>Cardiovascular screening programme</title>
<p>The following examinations were carried out to assess cardiovascular health:<list list-type="bullet">
<list-item>
<p>blood pressure (BP) measurement</p>
</list-item>
<list-item>
<p>physical examination (including determination of the body mass index)</p>
</list-item>
<list-item>
<p>blood sampling (determination of indicators of lipid and glucose metabolism)</p>
</list-item>
<list-item>
<p>calculation of the Prospective Cardiovascular M&#x00FC;nster Score (PROCAM score).</p>
</list-item>
</list></p>
<sec id="sec12">
<label>2.1.4.1</label>
<title>Blood pressure measurement</title>
<p>BP measurement was used in particular for the early detection of high BP (hypertension). The recommendations of Pickering et al. (<xref ref-type="bibr" rid="ref61">61</xref>) were followed and a calibrated BP measuring device (BOSO medicus from Bosch + Sohn GmbH u. Co. KG) was used for the measurement (practice BP measurement). BP was measured by healthcare professionals after about 5&#x202F;min of rest in a sitting position &#x2013; on both the left and right upper arm; it was given in millimetres of mercury [mmHg]. After 2&#x202F;min, a repeat measurement was taken on the arm with the higher BP, which was used to categorise normotensive and hypertensive MPOs. Hypertensive BP was defined as values &#x2265;140/90&#x202F;mmHg (or/and), normotensive BP was defined as values &#x003C;140/90&#x202F;mmHg (<xref ref-type="bibr" rid="ref62">62</xref>). Even if hypertensive BP values measured once or twice are only suspected to be high BP, those affected were labelled as hypertensive in this study. Anyone taking antihypertensive medication was categorised as hypertensive per se.</p>
</sec>
<sec id="sec13">
<label>2.1.4.2</label>
<title>Physical examination</title>
<p>Body weight and height were measured according to the standardised STEPS protocol (<xref ref-type="bibr" rid="ref63">63</xref>) and the body mass index (BMI) was calculated using the following formula: BMI&#x202F;=&#x202F;body weight [kg]/height squared [m<sup>2</sup>]. Based on the criteria of the German Obesity Society (<xref ref-type="bibr" rid="ref64">64</xref>), the BMI was used to classify underweight (&#x003C;18.5&#x202F;kg/m<sup>2</sup>), normal weight (18.5&#x2013;24.9&#x202F;kg/m<sup>2</sup>), overweight (25.0&#x2013;29.9&#x202F;kg/m<sup>2</sup>) and obesity (&#x2265;30.0&#x202F;kg/m<sup>2</sup>).</p>
</sec>
<sec id="sec14">
<label>2.1.4.3</label>
<title>Blood sampling</title>
<p>Total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C), triglycerides (TG) were examined as indicators of lipid metabolism and fasting serum glucose and glycated haemoglobin A1c (HbA1c) as indicators of carbohydrate metabolism, whereby LDL-C, HDL-C and TG were used as components of the PROCAM score. For their determination, venous blood was taken from the crook of the arm. Blood collection and transport of the blood samples and their analyses in the laboratory were carried out according to defined standards. Blood samples were taken on the day of the study between 05:30 and 08:00&#x202F;a.m. in a fasting state. The lipids were determined from the serum sample, the glycated haemoglobin from the plasma (anticoagulant ethylene diamine tetraacetic acid (EDTA)).</p>
<p>In practice, different standard ranges or cut-off values are known for the clinical interpretation of lipid and carbohydrate metabolism, whereby the diagnostic criteria vary depending on other risk factors and treatment goals. In addition, different units of measurement are used in the literature, which makes it difficult to compare the data. For example, the indicators of lipid metabolism are given in millimoles/litre [mmol/l] or milligrams/decilitre [mg/dl], HbA1c as a percentage of total haemoglobin [%] or in relation to 1 mole of haemoglobin in millimoles/mole of Hb [mmol/mol]. Irrespective of this, the classification of the American Association of Clinical Endocrinologists (AACE) (<xref ref-type="bibr" rid="ref65">65</xref>) was used for the clinical interpretation of lipids (<xref ref-type="table" rid="tab1">Table 1</xref>).</p>
<table-wrap position="float" id="tab1">
<label>Table 1</label>
<caption>
<p>Clinical evaluation of lipid metabolism indicators for men according to Jellinger et al. (<xref ref-type="bibr" rid="ref65">65</xref>).</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top" rowspan="2">Category</th>
<th align="center" valign="top" colspan="4">Classification of the indicators of lipid metabolism (mg/dl [mmol/l])</th>
</tr>
<tr>
<th align="center" valign="top">Normal</th>
<th align="center" valign="top">Borderline</th>
<th align="center" valign="top">Elevated/decreased</th>
<th align="center" valign="top">High</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top">Total cholesterol</td>
<td align="center" valign="top">&#x003C;200 [&#x003C;5.2]</td>
<td align="center" valign="top">200&#x2013;239 [5.2&#x2013;6.2]</td>
<td align="center" valign="top">&#x003E;239 [&#x003E;6.2]</td>
<td/>
</tr>
<tr>
<td align="left" valign="top">LDL cholesterol</td>
<td align="center" valign="top">&#x003C;130 [&#x003C;3.3]</td>
<td align="center" valign="top">130&#x2013;159 [3.3&#x2013;4.1]</td>
<td align="center" valign="top">&#x003E;159&#x2013;189 [&#x003E;4.1&#x2013;4.9]</td>
<td align="center" valign="top">&#x003E;189 [&#x003E;4.9]</td>
</tr>
<tr>
<td align="left" valign="top">HDL cholesterol</td>
<td align="center" valign="top">&#x003E;60 [&#x003E;1.5]</td>
<td align="center" valign="top">60&#x2013;40 [1.5&#x2013;1.0]</td>
<td align="center" valign="top">&#x003C;40 [&#x003C;1.0]</td>
<td/>
</tr>
<tr>
<td align="left" valign="top">Trigylcerides</td>
<td align="center" valign="top">&#x003C;150 [&#x003C;1.7]</td>
<td align="center" valign="top">150&#x2013;199 [1.7&#x2013;2.2]</td>
<td align="center" valign="top">&#x003E;199&#x2013;481 [&#x003E;2.2&#x2013;5.6]</td>
<td align="center" valign="top">&#x003E;481 [&#x003E;5.6]</td>
</tr>
</tbody>
</table>
</table-wrap>
<p>The HbA1c value is the proportion of native haemoglobin with glucose binding and was examined to assess the risk or the presence of diabetes mellitus. It provides information on the level and duration of elevated glucose levels in the plasma over the last four to 12&#x202F;weeks (long-term blood glucose value). In healthy adults, HbA1c values below 39&#x202F;mmol/mol (&#x003C;5.7%) are considered normal, HbA1c values between 39 and 48&#x202F;mmol/mol (5.7&#x2013;6.4%) indicate impaired blood glucose regulation (prediabetes), while HbA1c values above 48&#x202F;mmol/mol (&#x003E;6.4%) are assumed to indicate diabetes mellitus (<xref ref-type="bibr" rid="ref64">64</xref>).</p>
</sec>
<sec id="sec15">
<label>2.1.4.4</label>
<title>PROCAM score</title>
<p>The PROCAM score (<xref ref-type="bibr" rid="ref66">66</xref>) was used to estimate the ten-year risk of myocardial infarction. It is based on the epidemiological PROCAM study and applies to women and men aged between 20 and 75. The following eight factors are included in its calculation, each of which contributes independently to the individual risk of heart attack: age [years], gender, smoking status, presence of diabetes and/or heart attack in the family (first-degree relatives), systolic BP [mmHg] (taking into account the use of antihypertensives), blood concentration of HDL-C and LDL-C [mg/dl] and the TG [mg/dl]. Depending on the severity of the risk factors, different point values are assigned and then added to the PROCAM score (total score). According to Assmann et al. (<xref ref-type="bibr" rid="ref66">66</xref>), 0&#x2013;47 pts represent a low (&#x003C;10%), 48&#x2013;56 pts a medium (10&#x2013;20%) and more than 56 pts a high PROCAM risk (&#x003E;20%), i.e., the lower the PROCAM score, the lower the risk of suffering a heart attack in the next 10&#x202F;years.</p>
</sec>
</sec>
</sec>
<sec id="sec16">
<label>2.2</label>
<title>Data control and statistical analyses</title>
<p>Prior to the statistical calculations, the entire dataset was analysed for implausible information and statistical outliers in the data. There were no missing values for the questionnaire scales. For physiological measurements, missing values were replaced by the group mean. The statistical analyses of the data were carried out using the &#x201C;Statistical Package for the Social Science&#x201D; programme (SPSS, version 28) for Windows. First, the indicators for sleep, cardiovascular health and work-life balance were analysed descriptively (means, standard deviations, medians, quartiles, frequencies, percentages) and examined for differences between the two groups. Simple mean differences were analysed for continuous variables using the <italic>t</italic>-test for independent samples and for categorical variables using the <italic>chi</italic><sup>2</sup> test and the <italic>contingency coefficient</italic> (<italic>CC</italic>) (<xref ref-type="bibr" rid="ref67">67</xref>). In addition, significance tests were carried out for continuous variables within a group for the survey time points (T1:T5) using the <italic>t</italic>-test for dependent samples.</p>
<p>Previous research has shown that the effects of shift work on sleep, health and work-life balance are affected by numerous confounding factors (covariates): <italic>years in shift work, age, regular exercise, children in the household, chronotype</italic>. In a preliminary analysis, associations between these covariates and the independent variables sleep, health and work-life balance, as well as between baseline and follow-up measurements, were analysed using Spearman&#x2019;s rank correlation coefficient (<italic>R</italic>) and interpreted according to B&#x00FC;hl (<xref ref-type="bibr" rid="ref68">68</xref>).</p>
<p>To analyse changes the indicators between the survey time points (T1:T5) in both groups, <italic>two-factor analyses of covariance with repeated measures (rANCOVA</italic>: within-subject factor <italic>time,</italic> between-subject factor <italic>group</italic>) were calculated. As a result of this correlation analysis, the covariates to be considered later in the <italic>rANCOVA</italic> were determined, with associations of <italic>R</italic> &#x003E;&#x202F;0.20 being considered relevant for inclusion as covariates.</p>
<p>The <italic>analysis of variance</italic> is considered to be relatively robust against violations of its application assumptions (normal distribution of dependent variables and variance homogeneity between the groups, <italic>Levene</italic> test) (<xref ref-type="bibr" rid="ref67">67</xref>).</p>
<p>An error probability of <italic>&#x03B1;</italic>&#x202F;&#x003C;&#x202F;0.05 was defined as the statistical significance criterion and supplemented by effect sizes. Their interpretation was based on the conventions of Cohen (<xref ref-type="bibr" rid="ref69">69</xref>). Small effect sizes from <italic>d</italic>&#x202F;&#x2265;&#x202F;0.20 in the t-test or <italic>&#x03C7;</italic><sup>2</sup>-test and from <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;&#x2265;&#x202F;0.01 in the <italic>rANCOVA</italic> are considered practically relevant effects. The effect size <italic>d</italic> was calculated according to the formulas of Lenhard and Lenhard (<xref ref-type="bibr" rid="ref70">70</xref>).</p>
<sec id="sec17">
<label>2.2.1</label>
<title>Sample estimation</title>
<p>Before the start of the study, a sample estimation was carried out using the G&#x002A;Power programme (<xref ref-type="bibr" rid="ref71">71</xref>). It was expected that working in a rotating 12-h shift system over a period of 4&#x202F;years would have a significant impact on sleep quality and quantity. However, we found no effect sizes in the few longitudinal studies that investigated the effects of shift work on sleep. We therefore used the longitudinal study by &#x00C5;kerstedt et al. (<xref ref-type="bibr" rid="ref72">72</xref>) as a reference, which found a significant increase in the risk of difficulty falling asleep [odds ratio (OR = 2.8, CI = 1.84.5) for shift work compared to day work.</p>
<p>Accordingly, an ANOVA with repeated measures over a period of 4&#x202F;years (T1, T5) with an effect size of <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.14 (corresponds to <italic>f</italic>&#x202F;=&#x202F;0.403) and a power of 0.90 would require a total of 67 study participants to obtain a significant result (<italic>&#x03B1;</italic>&#x202F;=&#x202F;0.05). A drop-out rate of 20% was assumed here. We have based this on the dropout rate from clinical studies and our own experience in shift work research.</p>
</sec>
</sec>
</sec>
<sec sec-type="results" id="sec18">
<label>3</label>
<title>Results</title>
<p>The study investigated whether work in a rotating 12-h shift system compared to day work over a period of 4&#x202F;years (T5) led to poorer sleep quality and quantity, whether the cardiovascular risk indicators increased, or whether the work-life balance changed. Firstly, the sample is described. After that, the results of the baseline measurement (T1) are shown for each of these constructs in a comparison of shift and day workers (<italic>t</italic>-test for independent samples, <italic>chi</italic><sup>2</sup> test, <italic>contingency coefficient</italic>). Finally, the results of the repeat measurements (rANCOVAs) are presented for the respective constructs using the multivariate tests (Hotelling&#x2019;s Trace).</p>
<p>The main and interaction effects between baseline and follow-up measurement (factor <italic>time</italic>) and the two groups (factor <italic>group</italic>), as well as the covariates are considered in the following.</p>
<p>As a result of the preliminary analysis, the covariates were included in the <italic>rANCOVAs</italic> as follows: <italic>age</italic> was included as a confounding variable for the sleep and cardiovascular health indicators (exception: for the PROCAM score). For the work-life balance the covariate children living in the household <italic>(children/household [number]</italic>) was included in the repeated measurement models. Since very high correlations (<italic>R</italic>&#x202F;&#x2265;&#x202F;0.95) were confirmed for these covariates between T1 and T5, only the data from T1 were included in the analyses. The covariates <italic>chronotype</italic>, <italic>years in shift work</italic> (shift workers only) and <italic>regular sport/week</italic> were omitted as no practically significant correlations were found for these variables in the preliminary analysis for the sleep and health indicators.</p>
<sec id="sec19">
<label>3.1</label>
<title>Sample</title>
<p>The sample consisted of 45 MPOs who worked in a 12-h rotating shift system (06:00&#x2013;18:00&#x202F;h&#x2014;morning shift, 18:00&#x2013;06:00&#x202F;h&#x2014;night shift) with a night shift component and 30 MPOs who only worked 8&#x202F;h during the day (06:00&#x2013;14:30&#x202F;h&#x2014;day work), (hereinafter referred to as shift worker&#x2014;SW vs. day worker&#x2014;DW&#x202F;=&#x202F;groups). The 12-h shift system was a forward-rotating shift system with 1&#x202F;day off after the morning shifts and 3&#x202F;days off after the night shifts. All employees were employed full-time (40&#x202F;h/week). In addition to MPOs, the group of day workers also included electrical fitters, maintenance mechanics and engineers (hereinafter referred to as MPO).</p>
<p>At the baseline measurement, the average age of the sample was 40&#x202F;years. At this point, the shift workers had already been working shifts for an average of 9&#x202F;years, while the day workers had been in their current job for around 1&#x202F;year. They had always worked during the day in their previous careers. Overall, the MPOs had worked for an average of 23&#x202F;years (<italic>p</italic>&#x202F;=&#x202F;0.595).</p>
<sec id="sec20">
<label>3.1.1</label>
<title>Relationship status</title>
<p>The two groups did not differ in marital status or children living in the household (<italic>p</italic>&#x202F;&#x003E;&#x202F;0.05). In the sample, 80% of the MPOs lived with a partner (T5: 83%); more than half of them (57%) had no children in the household (T5: 53%). On average, shift workers had two children living in their households, while day workers had just one (T5&#x2014;SW vs. DW: 2 children vs. 1 child). Further characteristics of the sample can be found in <xref ref-type="table" rid="tab2">Table 2</xref>.</p>
<table-wrap position="float" id="tab2">
<label>Table 2</label>
<caption>
<p>Sample characteristics of the shift and day workers at baseline.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Sample characteristic</th>
<th align="center" valign="top">Dimension</th>
<th align="center" valign="top">Shift work (<italic>n</italic> =&#x202F;45)</th>
<th align="center" valign="top">Day work (<italic>n</italic> =&#x202F;30)</th>
<th align="center" valign="top">Test-value</th>
<th align="center" valign="top"><italic>p</italic>-value</th>
<th align="center" valign="top">Effect size <italic>d</italic></th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" colspan="7">Personal and work-related status</td>
</tr>
<tr>
<td align="left" valign="top">Age [years]</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">40.0&#x202F;&#x00B1;&#x202F;10.4</td>
<td align="center" valign="top">40.6&#x202F;&#x00B1;&#x202F;11.3</td>
<td align="center" valign="top">0.25</td>
<td align="center" valign="top">0.730</td>
<td align="center" valign="top">0.06</td>
</tr>
<tr>
<td align="left" valign="top">Duration of employment [years]</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">22.9&#x202F;&#x00B1;&#x202F;10.8</td>
<td align="center" valign="top">21.4&#x202F;&#x00B1;&#x202F;12.3</td>
<td align="center" valign="top">0.53</td>
<td align="center" valign="top">0.595</td>
<td align="center" valign="top">0.13</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="2">Duration of shift work [years]</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">8.7&#x202F;&#x00B1;&#x202F;10.0</td>
<td rowspan="2"/>
<td rowspan="2"/>
<td rowspan="2"/>
<td rowspan="2"/>
</tr>
<tr>
<td align="center" valign="top">Mdn (Q<sub>25</sub>, Q<sub>75</sub>)</td>
<td align="center" valign="top">9 (5, 11)</td>
</tr>
<tr>
<td align="left" valign="top" colspan="7">Relationship status</td>
</tr>
<tr>
<td align="left" valign="top">Permanent partnership</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">35 (77.8)</td>
<td align="center" valign="top">25 (83.3)</td>
<td align="center" valign="top">0.35</td>
<td align="center" valign="top">0.841</td>
<td align="center" valign="top">0.14</td>
</tr>
<tr>
<td align="left" valign="top">With parents/parents-in-law</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">4 (8.9)</td>
<td align="center" valign="top">2 (6.7)</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">Single</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">6 (13.3)</td>
<td align="center" valign="top">3 (10.0)</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top" colspan="7">Children and relatives</td>
</tr>
<tr>
<td align="left" valign="top">Children in the household [yes]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">22 (48.9)</td>
<td align="center" valign="top">10 (33.3)</td>
<td align="center" valign="top">1.78</td>
<td align="center" valign="top">0.182</td>
<td align="center" valign="top">0.31</td>
</tr>
<tr>
<td align="left" valign="top">Children in the household [number]</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">1.8&#x202F;&#x00B1;&#x202F;0.8</td>
<td align="center" valign="top">1.0&#x202F;&#x00B1;&#x202F;0.0</td>
<td align="center" valign="top">4.47</td>
<td align="center" valign="top">&#x003C;0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="top">1.14</td>
</tr>
<tr>
<td align="left" valign="top">Care of relatives [yes]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">4 (9.8)</td>
<td align="center" valign="top">&#x2013; (&#x2014;)</td>
<td align="center" valign="top">0.28</td>
<td align="center" valign="top">0.093</td>
<td align="center" valign="top">0.12</td>
</tr>
<tr>
<td align="left" valign="top" colspan="7">Health behaviour</td>
</tr>
<tr>
<td align="left" valign="top" colspan="7">Smoking status</td>
</tr>
<tr>
<td align="left" valign="top">Smoking [yes]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">15 (33.3)</td>
<td align="center" valign="top">9 (30.0)</td>
<td align="center" valign="top">0.09</td>
<td align="center" valign="top">0.762</td>
<td align="center" valign="top">0.07</td>
</tr>
<tr>
<td align="left" valign="top">Cigarettes [number/day]</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">12.5&#x202F;&#x00B1;&#x202F;6.8</td>
<td align="center" valign="top">11.2&#x202F;&#x00B1;&#x202F;6.8</td>
<td align="center" valign="top">0.46</td>
<td align="center" valign="top">0.653</td>
<td align="center" valign="top">0.19</td>
</tr>
<tr>
<td align="left" valign="top" colspan="7">Alcohol use</td>
</tr>
<tr>
<td align="left" valign="top">Regular alcohol consumption [yes]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">28 (62.2)</td>
<td align="center" valign="top">21 (70.0)</td>
<td align="center" valign="top">0.51</td>
<td align="center" valign="top">0.777</td>
<td align="center" valign="top">0.17</td>
</tr>
<tr>
<td align="left" valign="top">Quantity of alcohol [g/day]</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">11.0&#x202F;&#x00B1;&#x202F;6.9</td>
<td align="center" valign="top">13.0&#x202F;&#x00B1;&#x202F;6.7</td>
<td align="center" valign="top">1.02</td>
<td align="center" valign="top">0.157</td>
<td align="center" valign="top">0.29</td>
</tr>
<tr>
<td align="left" valign="top" colspan="7">Sporting activity</td>
</tr>
<tr>
<td align="left" valign="top">Regular sport/week</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">25 (56.6)</td>
<td align="center" valign="top">24 (80.0)</td>
<td align="center" valign="top">4.75</td>
<td align="center" valign="top">0.029&#x002A;</td>
<td align="center" valign="top">0.52</td>
</tr>
<tr>
<td align="left" valign="top">Time for sport [min/week]</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">204.0&#x202F;&#x00B1;&#x202F;121.9</td>
<td align="center" valign="top">238.8&#x202F;&#x00B1;&#x202F;125.3</td>
<td align="center" valign="top">0.98</td>
<td align="center" valign="top">0.165</td>
<td align="center" valign="top">0.28</td>
</tr>
<tr>
<td align="left" valign="top" colspan="7">Chronotype</td>
</tr>
<tr>
<td align="left" valign="top">Chronotype</td>
<td align="center" valign="top"><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD</td>
<td align="center" valign="top">58.6&#x202F;&#x00B1;&#x202F;8.9</td>
<td align="center" valign="top">58.7&#x202F;&#x00B1;&#x202F;8.7</td>
<td align="center" valign="top">0.07</td>
<td align="center" valign="top">0.945</td>
<td align="center" valign="top">0.02</td>
</tr>
<tr>
<td align="left" valign="top">Definitely evening type [16&#x2013;30 pts]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">1 (2.2)</td>
<td align="center" valign="top">&#x2013; (&#x2014;)</td>
<td align="center" valign="top">4.81</td>
<td align="center" valign="top">0.308</td>
<td align="center" valign="top">0.52</td>
</tr>
<tr>
<td align="left" valign="top">Moderately evening type [31&#x2013;41 pts]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">&#x2013; (&#x2014;)</td>
<td align="center" valign="top">(6.7)</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">Neither type [42&#x2013;58 pts]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">18 (40.0)</td>
<td align="center" valign="top">(46.7)</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">Moderately morning type [59&#x2013;69 pts]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">22 (48.9)</td>
<td align="center" valign="top">(43.3)</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">Definitely morning type [70&#x2013;86 pts]</td>
<td align="center" valign="top"><italic>n</italic> (%)</td>
<td align="center" valign="top">4 (8.9)</td>
<td align="center" valign="top">1 (3.3)</td>
<td/>
<td/>
<td/>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD: mean&#x202F;&#x00B1;&#x202F;standard deviation; Mdn (Q<sub>25</sub>, Q<sub>75</sub>): median (quartiles); <italic>n</italic> (%): sample size (frequency in percent). <italic>t</italic>-test (test size: <italic>t</italic>-value, effect size: <italic>d</italic>). <italic>n</italic> (%): <italic>chi</italic><sup>2</sup> test (test size: <italic>&#x03C7;</italic><sup>2</sup>-value, effect size: <italic>d</italic>). <italic>p</italic>-value: significance (two-sided): &#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.05, &#x002A;&#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.01. Effect size <italic>d</italic> according to Cohen (<xref ref-type="bibr" rid="ref69">69</xref>): &#x003C;0.20&#x202F;=&#x202F;no effect, 0.20&#x2013;0.49&#x202F;=&#x202F;small effect, 0.50&#x2013;0.79&#x202F;=&#x202F;medium effect, &#x2265;0.80&#x202F;=&#x202F;large effect.</p>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec21">
<label>3.1.2</label>
<title>Health behaviour</title>
<p>There were also no statistically significant differences for smoking and alcohol consumption (<italic>p</italic>&#x202F;&#x003E;&#x202F;0.05): 32% of the MPOs were smokers (T5: 31%) who smoked an average of 12 cigarettes/day (T5: M = 13 cigarettes/day). Overall, almost two thirds (65%) of the sample stated that they drank alcohol <italic>regularly</italic> (T5: 65%); 15% consumed alcohol <italic>occasionally</italic> (e.g., at parties). With regard to sporting activity, there was a significant difference (<italic>d</italic>&#x202F;=&#x202F;0.52&#x2014;medium effect) between the two groups: 57% of shift workers (T5: 60%) and 80&#x00B0;% of day workers (T5: 83%, <italic>d</italic>&#x202F;=&#x202F;0.51&#x2014;medium effect) exercised <italic>regularly</italic>, whereby the amount of time spent exercising did not differ between the two groups (<italic>p</italic>&#x202F;=&#x202F;0.165) and averaged 204 and 239&#x202F;min/week, respectively (T5: 217 and 207&#x202F;min/week).</p>
</sec>
<sec id="sec22">
<label>3.1.3</label>
<title>Chronotype</title>
<p>The expression of the individual chronotype was comparable in both groups (<italic>d</italic>&#x202F;=&#x202F;0.016). Their mean values ranged between &#x2018;neutral chronotype&#x2019; and &#x2018;moderate morning type&#x2019;, with most shift workers (49%) classified as &#x2018;moderate morning type&#x2019; and most day workers (47%) as &#x2018;neutral chronotype&#x2019; (<italic>p</italic>&#x202F;=&#x202F;0.308).</p>
</sec>
</sec>
<sec id="sec23">
<label>3.2</label>
<title>Sleep behaviour</title>
<p>The results of the sleep behaviour for the baseline and follow-up are summarised in <xref ref-type="table" rid="tab3">Table 3</xref> and <xref ref-type="fig" rid="fig1">Figure 1</xref>.</p>
<table-wrap position="float" id="tab3">
<label>Table 3</label>
<caption>
<p>Main and interaction effects of sleep quality (PSQI score) of shift and day workers at baseline and follow-up.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top" rowspan="2">Measurement time</th>
<th align="center" valign="top" rowspan="2">Shift work (<italic>n</italic> =&#x202F;45)</th>
<th align="center" valign="top" rowspan="2">Day work (<italic>n</italic> =&#x202F;30)</th>
<th align="center" valign="top" colspan="3">Within- and between-subject effects</th>
</tr>
<tr>
<th align="center" valign="top">Test-value</th>
<th align="center" valign="top"><italic>p</italic>-value</th>
<th align="center" valign="top">Effect size</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="middle">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD) [pts]</td>
<td align="center" valign="middle">4.4&#x202F;&#x00B1;&#x202F;1.5</td>
<td align="center" valign="middle">3.5&#x202F;&#x00B1;&#x202F;1.7</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;2.54</td>
<td align="center" valign="middle">0.017&#x002A;</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.58</td>
</tr>
<tr>
<td align="left" valign="middle" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD) [pts]</td>
<td align="center" valign="middle">4.8&#x202F;&#x00B1;&#x202F;2.8</td>
<td align="center" valign="middle">3.1&#x202F;&#x00B1;&#x202F;2.0</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;2.98</td>
<td align="center" valign="middle">0.004&#x002A;&#x002A;</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.66</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Group</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;9.05</td>
<td align="center" valign="middle">0.004&#x002A;&#x002A;</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.11</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Time</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;2.72</td>
<td align="center" valign="middle">0.137</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><italic>
<sub>p</sub>
</italic> &#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Time &#x002A; group</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;2.06</td>
<td align="center" valign="middle">0.155</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.03</td>
</tr>
<tr>
<td align="left" valign="middle">Covariate</td>
<td align="center" valign="middle" colspan="2">Age</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;1.45</td>
<td align="center" valign="middle">0.232</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.02</td>
</tr>
</tbody>
</table>
<table frame="hsides" rules="groups">
<tbody>
<tr>
<td rowspan="2"/>
<td align="center" valign="middle" colspan="2">Shift work</td>
<td colspan="3" rowspan="2"/>
</tr>
<tr>
<td align="center" valign="middle">Morning shift (<italic>n</italic> =&#x202F;45)</td>
<td align="center" valign="middle">Night shift (<italic>n</italic> =&#x202F;45)</td>
</tr>
<tr>
<td align="left" valign="middle">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD) [pts]</td>
<td align="center" valign="middle">4.4&#x202F;&#x00B1;&#x202F;1.5</td>
<td align="center" valign="middle">4.0&#x202F;&#x00B1;&#x202F;1.8</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;1.73</td>
<td align="center" valign="middle">0.091</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.26</td>
</tr>
<tr>
<td align="left" valign="middle" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD) [pts]</td>
<td align="center" valign="middle">4.8&#x202F;&#x00B1;&#x202F;2.8</td>
<td align="center" valign="middle">5.1&#x202F;&#x00B1;&#x202F;2.4</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;1.00</td>
<td align="center" valign="middle">0.161</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.15</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Shift</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;0.47</td>
<td align="center" valign="middle">0.496</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Time</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;1.06</td>
<td align="center" valign="middle">0.309</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.03</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Shift &#x002A; time</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;6.52</td>
<td align="center" valign="middle">0.014&#x002A;</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.13</td>
</tr>
<tr>
<td align="left" valign="middle">Covariate</td>
<td align="center" valign="middle" colspan="2">Age</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;0.74</td>
<td align="center" valign="middle">0.395</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.02</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>PSQ1: Pittsburgh Sleep Quality Index (PSQI score [pts]). <italic>M</italic>&#x202F;&#x00B1;&#x202F;SD: mean &#x00B1; standard deviation, <italic>n</italic>: sample size. <italic>t</italic>-test (test size: <italic>t</italic>-value, effect size: <italic>d</italic>). <italic>rANCOVA</italic> (test size: <italic>F</italic>-value, effect size: <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>): design: constant term + group + age; within-subjects effect: time (T1, T5). <italic>p</italic>-value: significance (two-sided): &#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.05, &#x002A;&#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.01. Effect size according to Cohen (<xref ref-type="bibr" rid="ref69">69</xref>): <italic>d</italic>: &#x003C;0.20&#x202F;=&#x202F;no effect, 0.20&#x2013;0.49&#x202F;=&#x202F;small effect, 0.50&#x2013;0.79&#x202F;=&#x202F;medium effect, &#x2265;0.80&#x202F;=&#x202F;large effect. <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>: &#x003C;0.01&#x202F;=&#x202F;no effect, 0.01&#x2013;0.05&#x202F;=&#x202F;small effect, 0.06&#x2013;0.13&#x202F;=&#x202F;medium effect, &#x2265;0.14&#x202F;=&#x202F;large effect.</p>
</table-wrap-foot>
</table-wrap>
<fig position="float" id="fig1">
<label>Figure 1</label>
<caption>
<p>Classification of sleep quality (PSQI score) of shift and day workers at baseline and follow-up (T1, T5). Notes. PSQI: Pittsburgh Sleep Quality Index [PSQI score (pts)]. <italic>n</italic>: sample size. <italic>chi</italic><sup>2</sup> test (test size: <italic>&#x03C7;</italic><sup>2</sup>-value, effect size: Cohen&#x2019;s <italic>d</italic>). <italic>p</italic>-value: significance (two-sided): <italic>p</italic>&#x202F;&#x003E;&#x202F;0.05 &#x2013; no significance. Effect size according to Cohen (<xref ref-type="bibr" rid="ref69">69</xref>): <italic>d</italic>: &#x003C;0.20&#x202F;=&#x202F;no effect, 0.20&#x2013;0.49&#x202F;=&#x202F;small effect, 0.50&#x2013;0.79&#x202F;=&#x202F;medium effect, &#x2265;0.80&#x202F;=&#x202F;large effect. Baseline: <italic>&#x03C7;</italic><sup>2</sup>(1)&#x202F;=&#x202F;3.12, <italic>p</italic>&#x202F;=&#x202F;0.077, <italic>d</italic>&#x202F;=&#x202F;0.42, follow-up: <italic>&#x03C7;</italic><sup>2</sup>(1)&#x202F;=&#x202F;4.46, <italic>p</italic>&#x202F;=&#x202F;0.101, <italic>d</italic>&#x202F;=&#x202F;0.50.</p>
</caption>
<graphic xlink:href="fpubh-13-1616810-g001.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Bar chart depicting sleep quality (PSQI score) in percentages for four groups: Shift work T1, Day work T1, Shift work T5, and Day work T5. Good sleep dominates in all groups. Shift work T1: 69% good, 31% bad; Day work T1: 87% good, 13% bad; Shift work T5: 67% good, 27% bad, 6% chronic disturbances; Day work T5: 87% good, 13% bad.</alt-text>
</graphic>
</fig>
<sec id="sec24">
<label>3.2.1</label>
<title>Baseline</title>
<p>For sleep behaviour, significantly lower sleep quality was confirmed for shift workers compared to day workers (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.58&#x2014;large effect). Thirty-one percent of shift workers and 13% of day workers fell into the <italic>bad sleep</italic> category (<italic>&#x03C7;</italic><sup>2</sup>(1)&#x202F;=&#x202F;3.12, <italic>p</italic>&#x202F;=&#x202F;0.077, <italic>d</italic>&#x202F;=&#x202F;0.42&#x2014;small effect). In the morning, shift workers went to bed at 21:48&#x202F;h on average (SD&#x202F;=&#x202F;3&#x202F;h 6&#x202F;min), day workers at 21:36&#x202F;h (SD&#x202F;=&#x202F;3&#x202F;h 54&#x202F;min, <italic>t</italic>(74)&#x202F;=&#x202F;0.28, <italic>p</italic>&#x202F;=&#x202F;0.782). Shift workers reported an average rising time of 04:25&#x202F;h (SD&#x202F;=&#x202F;30&#x202F;min), day workers 05:48&#x202F;h (SD&#x202F;=&#x202F;54&#x202F;min, <italic>t</italic>(74)&#x202F;=&#x202F;7.90, <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001, <italic>d</italic>&#x202F;=&#x202F;2.05&#x2014;large effect). At an average of 6&#x202F;h 6&#x202F;min (SD&#x202F;=&#x202F;1&#x202F;h), the sleep duration of shift workers was significantly shorter than that of day workers at 7&#x202F;h 18&#x202F;min (SD&#x202F;=&#x202F;48&#x202F;min, <italic>t</italic>(74)&#x202F;=&#x202F;5.28, <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001, <italic>d</italic>&#x202F;=&#x202F;1.38&#x2014;large effect). However, a distinction must be made between morning and night shift work: after the night shift, workers went to bed at 07:12&#x202F;h on average (SD&#x202F;=&#x202F;36&#x202F;min) and rose at 12:54&#x202F;h (SD&#x202F;=&#x202F;1&#x202F;h 12&#x202F;min); they slept on average only 5&#x202F;h 36&#x202F;min (SD&#x202F;=&#x202F;1&#x202F;h 24&#x202F;min) and thus 30&#x202F;min less than during the morning shift (<italic>t</italic>(44)&#x202F;=&#x202F;1.92, <italic>p</italic>&#x202F;=&#x202F;0.061, <italic>d</italic>&#x202F;=&#x202F;0.28). For shift workers, there was only a medium (<italic>R</italic>&#x202F;=&#x202F;0.63) correlation for the PSQI score between morning and night shift.</p>
</sec>
<sec id="sec25">
<label>3.2.2</label>
<title>Follow-up</title>
<p>For the follow-up measurement, shift workers also reported lower sleep quality for the morning shift (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.11&#x2014;medium effect) than day workers, although this did not change significantly in either group over the four-year period (<italic>p</italic>&#x202F;=&#x202F;0.137); there was no significant interaction between the survey time points T1 and T5 (factor <italic>time</italic> &#x002A; <italic>group</italic>, <italic>p</italic>&#x202F;=&#x202F;0.155). However, there was a significant interaction effect for the sleep quality of shift workers after morning and night shifts at both survey times T1 and T5 (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.13&#x2014;medium effect), according to which the PSQI score had practically deteriorated significantly after the night shift in 4&#x202F;years (<italic>d</italic>&#x202F;=&#x202F;0.46&#x2014;small effect) and had reached the borderline of poor sleep quality (&#x2265;5 pts).</p>
<p>Among the shift workers, 27% reported poor sleep quality and 7% a chronic sleep disorder after the morning shift (T1: 31% poor sleep, <italic>&#x03C7;</italic><sup>2</sup>(1)&#x202F;=&#x202F;8.99, <italic>p</italic>&#x202F;=&#x202F;0.011, <italic>d</italic>&#x202F;=&#x202F;0.74&#x2014;medium effect); 31% of them reported poor sleep quality and 4% a chronic sleep disorder after the night shift (T1: 27% poor sleep, <italic>&#x03C7;</italic><sup>2</sup>(1)&#x202F;=&#x202F;8.83, <italic>p</italic>&#x202F;=&#x202F;0.007, <italic>d</italic>&#x202F;=&#x202F;0.73&#x2014;medium effect).</p>
<p>There were no significant changes between the baseline and follow-up measurement for bedtime (<italic>F</italic>(71)&#x202F;=&#x202F;0.35, <italic>p</italic>&#x202F;=&#x202F;0.563) and wake-up time (<italic>F</italic>(71)&#x202F;=&#x202F;0.39, <italic>p</italic>&#x202F;=&#x202F;0.534) in the morning shift resp. day work for any group. There was also no change in the duration of sleep for day workers, while for shift workers it was 18&#x202F;min shorter on average for the follow-up. This effect was practically significant (<italic>F</italic>(42)&#x202F;=&#x202F;7.90, <italic>p</italic>&#x202F;=&#x202F;0.007, <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.15&#x2014;large effect) and was mainly observed on the night shift (<italic>M</italic>&#x202F;=&#x202F;5&#x202F;h 18&#x202F;min, SD&#x202F;=&#x202F;1&#x202F;h 12&#x202F;min).</p>
<p>The PSQI score correlated slightly (<italic>R</italic>&#x202F;=&#x202F;0.49) between baseline and follow-up for shift workers on the morning shift and moderately (<italic>R</italic>&#x202F;=&#x202F;0.60) for day workers, while a high correlation (<italic>R</italic> = 0.72) was observed between morning and night shift. <italic>Age</italic> did not have a significant influence on sleep quality in any group at any measurement time (morning shift vs. day work: <italic>p</italic>&#x202F;=&#x202F;0.129, T1: SW: <italic>R</italic>&#x202F;=&#x202F;0.08, DW: &#x2212;0.02, T5: SW: <italic>R</italic>&#x202F;=&#x202F;0.08, DW: 0.25).</p>
</sec>
</sec>
<sec id="sec26">
<label>3.3</label>
<title>State of health</title>
<p>The results for the assessment of cardiovascular health are shown in <xref ref-type="table" rid="tab4">Table 4</xref> and <xref ref-type="fig" rid="fig2">Figure 2</xref>.</p>
<table-wrap position="float" id="tab4">
<label>Table 4</label>
<caption>
<p>Main and interaction effects of cardiovascular health indicators of shift and day workers at baseline and follow-up.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top" rowspan="2">Indicator</th>
<th rowspan="2"/>
<th align="center" valign="top" rowspan="2">Shift work (<italic>n</italic> =&#x202F;45)</th>
<th align="center" valign="top" rowspan="2">Day work (<italic>n</italic> =&#x202F;30)</th>
<th align="center" valign="top" colspan="3">Within- and between-subject effects</th>
</tr>
<tr>
<th align="center" valign="top">Test-value</th>
<th align="center" valign="top"><italic>p</italic>-value</th>
<th align="center" valign="top">Effect size</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" rowspan="6">Systolic blood pressure [mmHg]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">138.4&#x202F;&#x00B1;&#x202F;13.4</td>
<td align="center" valign="top">129.8&#x202F;&#x00B1;&#x202F;15.2</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;2.55</td>
<td align="center" valign="top">0.013&#x002A;</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.60</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">133.2&#x202F;&#x00B1;&#x202F;18.2</td>
<td align="center" valign="top">130.5&#x202F;&#x00B1;&#x202F;14.8</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;0.68</td>
<td align="center" valign="top">0.497</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.16</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;3.11</td>
<td align="center" valign="top">0.082</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.04</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.12</td>
<td align="center" valign="top">0.733</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;&#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;2.01</td>
<td align="center" valign="top">0.161</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.03</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;16.32</td>
<td align="center" valign="top">&#x003C;0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.19</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">Diastolic blood pressure [mmHg]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">82.2&#x202F;&#x00B1;&#x202F;4.9</td>
<td align="center" valign="top">79.2&#x202F;&#x00B1;&#x202F;7.8</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;2.08</td>
<td align="center" valign="top">0.041&#x002A;</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.49</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">80.7&#x202F;&#x00B1;&#x202F;7.7</td>
<td align="center" valign="top">80.5&#x202F;&#x00B1;&#x202F;5.8</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;0.10</td>
<td align="center" valign="top">0.920</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.02</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.11</td>
<td align="center" valign="top">0.296</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.02</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.63</td>
<td align="center" valign="top">0.430</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;2.77</td>
<td align="center" valign="top">0.101</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.04</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;16.05</td>
<td align="center" valign="top">&#x003C;0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.18</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">Body mass index [kg/m<sup>2</sup>]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">28.6&#x202F;&#x00B1;&#x202F;4.4</td>
<td align="center" valign="top">25.7&#x202F;&#x00B1;&#x202F;4.1</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;2.88</td>
<td align="center" valign="top">0.003&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.68</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">28.9&#x202F;&#x00B1;&#x202F;4.7</td>
<td align="center" valign="top">26.0&#x202F;&#x00B1;&#x202F;3.7</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;2.89</td>
<td align="center" valign="top">0.003&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.68</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;7.74</td>
<td align="center" valign="top">0.007&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.10</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.02</td>
<td align="center" valign="top">0.315</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.13</td>
<td align="center" valign="top">0.715</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;&#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;4.84</td>
<td align="center" valign="top">0.031&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.06</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">Cholesterol [mmol/l]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">5.3&#x202F;&#x00B1;&#x202F;1.1</td>
<td align="center" valign="top">5.4&#x202F;&#x00B1;&#x202F;1.1</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;0.52</td>
<td align="center" valign="top">0.607</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.12</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">5.4&#x202F;&#x00B1;&#x202F;1.5</td>
<td align="center" valign="top">5.6&#x202F;&#x00B1;&#x202F;1.2</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;0.46</td>
<td align="center" valign="top">0.644</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.11</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.68</td>
<td align="center" valign="top">0.411</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.35</td>
<td align="center" valign="top">0.492</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.02</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.05</td>
<td align="center" valign="top">0.822</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;&#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;2.52</td>
<td align="center" valign="top">0.117</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.03</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">LDL-Cholesterol [mmol/l]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">3.4&#x202F;&#x00B1;&#x202F;1.0</td>
<td align="center" valign="top">3.3&#x202F;&#x00B1;&#x202F;0.9</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;0.75</td>
<td align="center" valign="top">0.455</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.18</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">3.5&#x202F;&#x00B1;&#x202F;1.0</td>
<td align="center" valign="top">3.2&#x202F;&#x00B1;&#x202F;0.9</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;1.46</td>
<td align="center" valign="top">0.148</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.35</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.97</td>
<td align="center" valign="top">0.329</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;2.02</td>
<td align="center" valign="top">0.160</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.03</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.07</td>
<td align="center" valign="top">0.305</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.02</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.20</td>
<td align="center" valign="top">0.276</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.02</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">HDL-cholesterol [mmol/l]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">1.2&#x202F;&#x00B1;&#x202F;0.3</td>
<td align="center" valign="top">1.4&#x202F;&#x00B1;&#x202F;0.3</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;2.54</td>
<td align="center" valign="top">0.013&#x002A;</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.60</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">1.2&#x202F;&#x00B1;&#x202F;0.3</td>
<td align="center" valign="top">1.5&#x202F;&#x00B1;&#x202F;0.4</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;3.49</td>
<td align="center" valign="top">&#x003C;0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.82</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;11.24</td>
<td align="center" valign="top">0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.14</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.01</td>
<td align="center" valign="top">0.998</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;&#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.37</td>
<td align="center" valign="top">0.246</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.02</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.07</td>
<td align="center" valign="top">0.799</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;&#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">Triglycerides [mmol/l]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">2.2&#x202F;&#x00B1;&#x202F;1.0</td>
<td align="center" valign="top">1.9&#x202F;&#x00B1;&#x202F;0.9</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;1.07</td>
<td align="center" valign="top">0.287</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.25</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">2.3&#x202F;&#x00B1;&#x202F;1.3</td>
<td align="center" valign="top">2.1&#x202F;&#x00B1;&#x202F;0.8</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;1.04</td>
<td align="center" valign="top">0.259</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.25</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;2.21</td>
<td align="center" valign="top">0.141</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.03</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;2.91</td>
<td align="center" valign="top">0.092</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.04</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.15</td>
<td align="center" valign="top">0.699</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;&#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.63</td>
<td align="center" valign="top">0.431</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.01</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">Glycated haemoglobin [mmol/mol]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">39.5&#x202F;&#x00B1;&#x202F;9.9</td>
<td align="center" valign="top">38.6&#x202F;&#x00B1;&#x202F;8.1</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;0.44</td>
<td align="center" valign="top">0.663</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.10</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">38.1&#x202F;&#x00B1;&#x202F;9.5</td>
<td align="center" valign="top">37.8&#x202F;&#x00B1;&#x202F;7.3</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;0.18</td>
<td align="center" valign="top">0.849</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.04</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.26</td>
<td align="center" valign="top">0.265</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.02</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;6.59</td>
<td align="center" valign="top">0.012&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.09</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;1.87</td>
<td align="center" valign="top">0.176</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.03</td>
</tr>
<tr>
<td align="left" valign="top">Covariate</td>
<td align="center" valign="top" colspan="2">Age</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;16.68</td>
<td align="center" valign="top">0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.19</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="5">PROCAM score [pts]</td>
<td align="left" valign="top">Baseline (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">30.3&#x202F;&#x00B1;&#x202F;12.7</td>
<td align="center" valign="top">25.4&#x202F;&#x00B1;&#x202F;14.4</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;1.56</td>
<td align="center" valign="top">0.122</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.37</td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">Follow-up (<italic>M</italic>&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="top">33.8&#x202F;&#x00B1;&#x202F;12.7</td>
<td align="center" valign="top">28.0&#x202F;&#x00B1;&#x202F;16.6</td>
<td align="center" valign="top"><italic>t</italic>&#x202F;=&#x202F;1.72</td>
<td align="center" valign="top">0.090</td>
<td align="center" valign="top"><italic>d</italic>&#x202F;=&#x202F;0.40</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;6.36</td>
<td align="center" valign="top">0.014&#x002A;</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.08</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.78</td>
<td align="center" valign="top">0.380</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="top" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic>&#x202F;=&#x202F;0.57</td>
<td align="center" valign="top">0.451</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.01</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>M</italic>&#x202F;&#x00B1;&#x202F;SD: mean&#x202F;&#x00B1;&#x202F;standard deviation, <italic>n</italic>: sample size. <italic>t</italic>-test (test size: <italic>t</italic>-value, effect size: <italic>d</italic>). <italic>rANCOVA</italic> (test size: <italic>F</italic>-value, effect size: <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>): design: constant term + shift group + age (exception PROCAM score); within-subjects effect: time (T1, T5). <italic>p</italic>-value: significance (two-sided): &#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.05, &#x002A;&#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.01, &#x002A;&#x002A;&#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001. Effect size according to Cohen (<xref ref-type="bibr" rid="ref69">69</xref>): <italic>d</italic>: &#x003C;0.20&#x202F;=&#x202F;no effect, 0.20&#x2013;0.49&#x202F;=&#x202F;small effect, 0.50&#x2013;0.79&#x202F;=&#x202F;medium effect, &#x2265;0.80&#x202F;=&#x202F;large effect. <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>: &#x003C;0.01&#x202F;=&#x202F;no effect, 0.01&#x2013;0.05&#x202F;=&#x202F;small effect, 0.06&#x2013;0.14&#x202F;=&#x202F;medium effect.</p>
</table-wrap-foot>
</table-wrap>
<fig position="float" id="fig2">
<label>Figure 2</label>
<caption>
<p><bold>(A-H)</bold> Classification of cardiovascular health indicators (BP, BMI, TC, LDL-C, HDL-C, TG, HbA1c, PROCAM score) of shift and day workers at baseline and follow-up (T1, T5). <italic>n</italic>: sample size. Blood pressure: hypertensive: &#x2265;140/90&#x202F;mmHg (or/and) and intake of antihypertensive medication, normotensive: &#x003C;140/90&#x202F;mmHg (<xref ref-type="bibr" rid="ref62">62</xref>).</p>
</caption>
<graphic xlink:href="fpubh-13-1616810-g002.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Eight bar charts display the frequency percentages of different health metrics for shift work and day work groups at two time points, T1 and T5. (A) Blood pressure categories: normotensive and hypertensive. (B) Body mass index: normal weight, overweight, obesity. (C) Total cholesterol: normal, borderline, high. (D) LDL cholesterol: normal, borderline, high. (E) HDL cholesterol: normal, borderline, low. (F) Triglycerides: normal, borderline, high. (G) Glycated hemoglobin: normal, prediabetes, diabetes. (H) PROCAM score: less than 10%, 10-20%, greater than 20%. Each bar indicates the proportion of individuals in each category.</alt-text>
</graphic>
</fig>
<sec id="sec27">
<label>3.3.1</label>
<title>Baseline</title>
<p>At baseline, blood pressure (<italic>M</italic>&#x202F;=&#x202F;138/82 vs. 130/79&#x202F;mmHg, <italic>d</italic>&#x202F;=&#x202F;0.60&#x2014;medium effect) and body mass index (<italic>M</italic>&#x202F;=&#x202F;29 vs. 26&#x202F;kg/m<sup>2</sup>, <italic>d</italic>&#x202F;=&#x202F;0.68&#x2014;medium effect) were significantly higher on average for shift workers than for day workers and significantly lower for HDL cholesterol (<italic>M</italic>&#x202F;=&#x202F;1.2 vs. 1.4, <italic>d</italic>&#x202F;=&#x202F;0.60&#x2014;medium effect). The average blood pressure was classified as normal in both groups (<xref ref-type="bibr" rid="ref62">62</xref>). However, one third (33%) of shift workers, but only 10% of day workers, took antihypertensives. The proportion of hypertensive individuals was only slightly higher among shift workers than among day workers (42% vs. 27%, <italic>p</italic>&#x202F;=&#x202F;0.169). In MPOs who did not take antihypertensive drugs (SW: <italic>n</italic>&#x202F;=&#x202F;30, DW: <italic>n</italic>&#x202F;=&#x202F;27), the average blood pressure did not differ significantly between the two groups (<italic>M</italic>&#x202F;=&#x202F;132/80 vs. 129/80&#x202F;mmHg, <italic>p</italic>&#x202F;=&#x202F;0.392/0.800). Similarly, there were no group differences between MPOs taking antihypertensive drugs (<italic>M</italic>&#x202F;=&#x202F;150/87 vs. 133/75&#x202F;mmHg, <italic>p</italic>&#x202F;=&#x202F;0.065/0.264). The mean values of the body mass index in both groups had reached the overweight range (<xref ref-type="bibr" rid="ref64">64</xref>). This affected about half of the shift workers (47%) and day workers (53%); obesity was present in 33% of the shift workers and 10% of the day workers (<italic>d</italic>&#x202F;=&#x202F;0.64&#x2014;medium effect). The mean values for HDL cholesterol in both groups had also reached borderline range (HDL-C: 1.5&#x2013;1.0&#x202F;mmol/l) (<xref ref-type="bibr" rid="ref65">65</xref>). Only 11% of shift workers and around a quarter (27%) of day workers had acceptable HDL cholesterol levels.</p>
<p>The mean values for total cholesterol, LDL cholesterol, triglycerides and HbA1c did not differ significantly between the two groups (<italic>p</italic>&#x202F;&#x003E;&#x202F;0.05). However, the mean values for lipid status (TC, LDL-C, TG) had reached the borderline range in both groups (<xref ref-type="bibr" rid="ref65">65</xref>). In each case, more than 40% of MPOs had elevated or high values for total cholesterol (&#x2265;5.2 mmol/l: 45%), LDL cholesterol (&#x2265;3.3&#x202F;mmol/l: 41%) and triglycerides (1.7&#x2013;2.2&#x202F;mmol/l: 45%). The mean HbA1c values in both groups had also reached the borderline of prediabetes (<xref ref-type="bibr" rid="ref64">64</xref>): 39&#x2013;48&#x202F;mmol/mol; 39% of MPOs were within the range of prediabetes and known diabetes was confirmed for 7%.</p>
<p>For the PROCAM score, there was a trend towards a higher cardiovascular risk for shift workers (<italic>p</italic>&#x202F;=&#x202F;0.122). The high standard deviations (6%) indicated a very different risk. However, the mean values of the PROCAM score in both groups were in the low to moderate range (<italic>M</italic>&#x202F;=&#x202F;30 vs. 25 pts), which predicts a low risk (0&#x2013;47 pts: &#x003C;10%) of a heart attack in the next 10&#x202F;years (<xref ref-type="bibr" rid="ref66">66</xref>). This was true for 87% of MPOs, 12% of MPOs were found to be at intermediate (48&#x2013;56 pts: 10&#x2013;20%) and 2% at high (&#x003E;56 pts: &#x003E;20%) cardiovascular risk (<italic>p</italic>&#x202F;=&#x202F;0.638). On average, the PROCAM risk was 4.9% for shift workers and 4.0% for day workers (<italic>t</italic>(73) = 0.66, <italic>p</italic>&#x202F;=&#x202F;0.509).</p>
</sec>
<sec id="sec28">
<label>3.3.2</label>
<title>Follow-up</title>
<p>After 4&#x202F;years, no significant interaction effect for <italic>time &#x002A; group</italic> was found for any of the health indicators analysed (<italic>p</italic>&#x202F;&#x003E;&#x202F;0.05). This suggests that these health indicators did not develop significantly differently in the two groups over the four-year period. The characteristics of the lipid metabolism and the risk of diabetes of the baseline measurement remained unchanged. However, a main effect for the <italic>group</italic> factor was found for the body mass index, HDL cholesterol and the PROCAM score. For these indicators, the significantly less favourable mean values of the shift workers at the baseline measurement were also found in the follow-up (T5: BMI: <italic>M</italic>&#x202F;=&#x202F;29 vs. 26&#x202F;kg/m<sup>2</sup>, <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.10&#x2014;medium effect, HDL-C: <italic>M</italic>&#x202F;=&#x202F;1.4 vs. 1.5&#x202F;mmol/l, <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.14&#x2014;large effect, PROCAM score: <italic>M</italic>&#x202F;=&#x202F;34 vs. 28 pts, <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.08&#x2014;medium effect). For the HbA1c, there was a significant main effect of the <italic>time</italic> factor (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.08&#x2014;medium effect), which showed a significant improvement in the average HbA1c values for the follow-up measurement.</p>
<p>Shift workers were also characterised by significantly lower mean blood pressure values for the follow-up. However, 42% of the shift workers were now taking antihypertensives (DW: 13%), which moderated blood pressure regulation. More than half (53%) of the MPOs were classified as persons with hypertension (T1:T5 (CC): SW: 62%, <italic>p</italic>&#x202F;&#x003C;&#x202F;0.001, DW: 40%, <italic>p</italic>&#x202F;=&#x202F;0.018).</p>
<p>MPOs who did not take antihypertensive drugs at either measurement point (SW: <italic>n</italic>&#x202F;=&#x202F;26, DW: <italic>n</italic>&#x202F;=&#x202F;26) showed no significant difference in average blood pressure between the two groups at the follow-up measurement (<italic>M</italic>&#x202F;=&#x202F;129/80 vs. 130/81&#x202F;mmHg, <italic>t</italic>(50)&#x202F;=&#x202F;0.856/0.827, <italic>p</italic>&#x202F;=&#x202F;0.856/0.827). There were no significant differences for the factors <italic>time</italic> (<italic>p</italic>&#x202F;=&#x202F;0.121/0.385), <italic>group</italic> (<italic>p</italic>&#x202F;=&#x202F;0.468/0.254) or <italic>time &#x002A; group</italic> (<italic>p</italic>&#x202F;=&#x202F;0.447/0.382). Accordingly, blood pressure did not change significantly in these two groups over 4&#x202F;years. Shift and day workers who were taking antihypertensive drugs at both measurement times had lower blood pressure at the follow-up measurement (<italic>M</italic>&#x202F;=&#x202F;143/83 vs. 130/78&#x202F;mmHg), but there were also no significant effects for the factors <italic>time</italic> (<italic>p</italic>&#x202F;=&#x202F;0.526/0.474), <italic>group</italic> (<italic>p</italic>&#x202F;=&#x202F;0.087/0.0.77) or <italic>time &#x002A; group</italic> (<italic>p</italic>&#x202F;=&#x202F;0.790/0.191).</p>
<p>As there were no effects of the <italic>time</italic> factor for any other health indicators, a comparable state can be assumed between baseline and follow-up. With the exception of blood pressure (SBP: <italic>R</italic>&#x202F;=&#x202F;0.37, DBP: <italic>r</italic>&#x202F;=&#x202F;0.29), this finding (<italic>R</italic>&#x202F;=&#x202F;0.83&#x2013;0.93) was confirmed in the correlation analysis.</p>
<p><italic>Age</italic> had a significant effect on blood pressure, body mass index and HbA1c (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>: SBP&#x202F;=&#x202F;0.19&#x2014;large effect, DBP&#x202F;=&#x202F;0.18&#x2014;large effect, BMI&#x202F;=&#x202F;0.06&#x2014;medium effect, HbA1c&#x202F;=&#x202F;0.19&#x2014;large effect). In both groups, the mean values of these indicators increased with <italic>age</italic> (T1: <italic>R</italic>&#x202F;=&#x202F;0.22&#x2013;0.61, T5: <italic>R</italic>&#x202F;=&#x202F;0.21&#x2013;0.67). <italic>Age</italic> had no influence on blood lipids (TC, LDL-C, HDL-C, TG: <italic>p</italic>&#x202F;&#x003E;&#x202F;0.05, T1: R&#x202F;=&#x202F;0.01&#x2013;0.18, T5: <italic>R</italic>&#x202F;=&#x202F;0.02&#x2013;0.09). The PROCAM score includes <italic>age</italic> itself as a risk factor.</p>
</sec>
</sec>
<sec id="sec29">
<label>3.4</label>
<title>Work-life balance</title>
<p>Work-life balance was analysed using the two scales satisfaction and impairment of the questionnaire for shift workers (<xref ref-type="bibr" rid="ref52">52</xref>) controlling for covariate (<italic>children/household [number]</italic>). The results of the work-life balance for the baseline and follow-up can be found in <xref ref-type="table" rid="tab5">Table 5</xref> and <xref ref-type="fig" rid="fig3">Figures 3a</xref>,<xref ref-type="fig" rid="fig3">b</xref>, whereby <xref ref-type="fig" rid="fig3">Figure 3a</xref> only reflects the work-related impairments to private life.</p>
<table-wrap position="float" id="tab5">
<label>Table 5</label>
<caption>
<p>Main and interaction effects of work-life-balance (scales satisfaction and impairment) of shift and day workers at baseline and follow-up.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top" rowspan="2">Indicator</th>
<th rowspan="2"/>
<th align="center" valign="top" rowspan="2">Shift work (<italic>n</italic> =&#x202F;45)</th>
<th align="center" valign="top" rowspan="2">Day work (<italic>n</italic> =&#x202F;30)</th>
<th align="center" valign="top" colspan="3">Within- and between-subject effect</th>
</tr>
<tr>
<th align="center" valign="top">Test-value</th>
<th align="center" valign="top"><italic>p</italic>-value</th>
<th align="center" valign="top">Effect size</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" rowspan="6">Satisfaction score [pts]</td>
<td align="left" valign="middle">Baseline (M&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="middle">14.1&#x202F;&#x00B1;&#x202F;5.4</td>
<td align="center" valign="middle">10.9&#x202F;&#x00B1;&#x202F;4.7</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;2.67</td>
<td align="center" valign="middle">0.009&#x002A;&#x002A;</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.63</td>
</tr>
<tr>
<td align="left" valign="middle" rowspan="4">Follow-up (M&#x202F;&#x00B1;&#x202F;SD)</td>
<td align="center" valign="middle">10.0&#x202F;&#x00B1;&#x202F;2.3</td>
<td align="center" valign="middle">11.2&#x202F;&#x00B1;&#x202F;3.6</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;1.58</td>
<td align="center" valign="middle">0.121</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.41</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Group</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;1.13</td>
<td align="center" valign="middle">0.292</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.02</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Time</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;11.30</td>
<td align="center" valign="middle">0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.14</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Time &#x002A; group</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;19.37</td>
<td align="center" valign="middle">&#x003C;0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.21</td>
</tr>
<tr>
<td align="left" valign="middle">Covariate</td>
<td align="center" valign="middle" colspan="2">Children/household [number]</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;0.16</td>
<td align="center" valign="middle">0.693</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> &#x003C;&#x202F;0.01</td>
</tr>
<tr>
<td align="left" valign="middle" rowspan="6">Impairment score [pts]</td>
<td align="left" valign="middle">Baseline (<italic>M</italic> &#x00B1;&#x202F;SD)</td>
<td align="center" valign="middle">19.0&#x202F;&#x00B1;&#x202F;3.0</td>
<td align="center" valign="middle">19.9&#x202F;&#x00B1;&#x202F;2.7</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;1.38</td>
<td align="center" valign="middle">0.172</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.33</td>
</tr>
<tr>
<td align="left" valign="middle" rowspan="4">Follow-up (<italic>M</italic> &#x00B1;&#x202F;SD)</td>
<td align="center" valign="middle">20.8&#x202F;&#x00B1;&#x202F;2.4</td>
<td align="center" valign="middle">21.4&#x202F;&#x00B1;&#x202F;2.8</td>
<td align="center" valign="middle"><italic>t</italic> =&#x202F;1.05</td>
<td align="center" valign="middle">0.299</td>
<td align="center" valign="middle"><italic>d</italic> =&#x202F;0.25</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Group</td>
<td align="center" valign="middle"><italic>F</italic> =&#x202F;0.61</td>
<td align="center" valign="top">0.436</td>
<td align="center" valign="top"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.01</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Time</td>
<td align="center" valign="top"><italic>F</italic> =&#x202F;22.17</td>
<td align="center" valign="middle">&#x003C;0.001&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.24</td>
</tr>
<tr>
<td align="center" valign="middle" colspan="2">Time &#x002A; group</td>
<td align="center" valign="top"><italic>F</italic> =&#x202F;0.41</td>
<td align="center" valign="middle">0.526</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.01</td>
</tr>
<tr>
<td align="left" valign="middle">Covariate</td>
<td align="center" valign="middle" colspan="2">Children/household [number]</td>
<td align="center" valign="top"><italic>F</italic> =&#x202F;5.28</td>
<td align="center" valign="middle">0.024&#x002A;</td>
<td align="center" valign="middle"><italic>&#x03B7;</italic><sup>2</sup><sub>p</sub> =&#x202F;0.07</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>n</italic>: sample size, <italic>M</italic>&#x202F;&#x00B1;&#x202F;SD: mean &#x00B1; standard deviation. <italic>t</italic>-test: (test size: <italic>t</italic>-value, effect size: <italic>d</italic>). <italic>rANCOVA</italic> (test size: <italic>F</italic>-value, effect size: <italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>): design: constant term + group + children/household [number]; within-subjects effect: time (T1, T5). <italic>p</italic>-value: significance (two-sided): &#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.05, &#x002A;&#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.01, &#x002A;&#x002A;&#x002A;<italic>p</italic>&#x202F;&#x003C;&#x202F;0.001. Effect size according to Cohen (<xref ref-type="bibr" rid="ref69">69</xref>): <italic>d</italic>: &#x003C;0.20&#x202F;=&#x202F;no effect, 0.20&#x2013;0.49&#x202F;=&#x202F;small effect, 0.50&#x2013;0.79&#x202F;=&#x202F;medium effect, &#x2265;0.80&#x202F;=&#x202F;large effect. <italic>&#x03B7;</italic><sup>2</sup><sub>p:</sub> &#x003C;0.01&#x202F;=&#x202F;no effect, 0.01&#x2013;0.05&#x202F;=&#x202F;small effect, 0.06&#x2013;0.14&#x202F;=&#x202F;medium effect, &#x2265;0.14&#x202F;=&#x202F;large effect.</p>
</table-wrap-foot>
</table-wrap>
<fig position="float" id="fig3">
<label>Figure 3</label>
<caption>
<p><bold>(a)</bold>Satisfactio with the time available for social and leisure activities of shift and day workers at baseline and follow-upT1, T5). <bold>(b)</bold> Impairment from work of shift and day workers at baseline and follow-up (T1, T5). SW: shift work, DW: day work.</p>
</caption>
<graphic xlink:href="fpubh-13-1616810-g003.tif" mimetype="image" mime-subtype="tiff">
<alt-text content-type="machine-generated">Two bar charts showing work impacts and time satisfaction. Chart (a) displays impairment from work on everyday activities, family time, and leisure energy, comparing shift work (SW) and day work (DW) at two time points (T1, T5) with frequency percentages for "never," "sometimes," "often." Chart (b) shows satisfaction percentages for activities like sleep, family, and hobbies among shift and day workers at T1 and T5.</alt-text>
</graphic>
</fig>
<sec id="sec30">
<label>3.4.1</label>
<title>Baseline</title>
<p>For the satisfaction score, a significant difference between shift and day workers was detectable at the baseline measurement (<italic>d</italic>&#x202F;=&#x202F;0.63&#x2014;medium effect), according to which shift workers were more dissatisfied with their available free time than day workers (<italic>M</italic>&#x202F;=&#x202F;14 vs. 11 of 24 pts). This effect was consequently also reflected in the individual items of the satisfaction in social and leisure-related activities items (<xref ref-type="fig" rid="fig3">Figure 3a</xref>): about half of the MPOs reported being satisfied with the time available for sleep (<italic>p</italic>&#x202F;=&#x202F;0.206). Shift workers complained most that they lacked time to participate in organisations or clubs; only about a quarter of them (DW: 40%) had regular time for such leisure activities (<italic>p</italic>&#x202F;=&#x202F;0.120). Similarly, only around a third of shift workers were satisfied with the amount of time available for friends and relatives (DW: 67%, <italic>d</italic>&#x202F;=&#x202F;0.76&#x2014;medium effect), cultural events (DW: 57%, <italic>d</italic>&#x202F;=&#x202F;0.76&#x2014;medium effect), hobbies and sporting activities (DW: 57%, <italic>d</italic>&#x202F;=&#x202F;0.69&#x2014;medium effect), as well as excursions and travelling (DW: 60%, <italic>p</italic>&#x202F;=&#x202F;0.085). About half were satisfied with the time they could spend with their partner (<italic>p</italic>&#x202F;=&#x202F;0.195) and family (<italic>p</italic>&#x202F;=&#x202F;0.106).</p>
<p>For the impairment score, there were no group differences at baseline (<italic>p</italic>&#x202F;=&#x202F;0.172), i.e., at the start of the study, shift and day workers were impaired by their work to the same extent (<italic>M</italic>&#x202F;=&#x202F;19 vs. 20 of 25 pts). This effect was only partially confirmed in the individual items (<xref ref-type="fig" rid="fig3">Figure 3b</xref>): for about half of MPOs, their private life was not affected by their work, for around a quarter <italic>frequently</italic> and <italic>occasionally</italic>, respectively (<italic>p</italic>&#x202F;=&#x202F;0.061). A third of shift workers were also frequently and more than half occasionally unable to spend their time with family or friends as they wished (DW: 10% vs. 80%, <italic>d</italic>&#x202F;=&#x202F;0.61&#x2014;medium effect). 8% of all MPOs <italic>frequently</italic> and 29% <italic>occasionally</italic> lacked the energy to enjoy their free time; 63% did not experience this problem (<italic>p</italic>&#x202F;=&#x202F;0.562).</p>
</sec>
<sec id="sec31">
<label>3.4.2</label>
<title>Follow-up</title>
<p>The time between the two survey points T1 and T5 did make a significant contribution to the explanation of the satisfaction with the time available for social and leisure activities scale for either shift or day workers (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.14&#x2014;medium effect). There was no significant main effect for <italic>group</italic> (<italic>p</italic>&#x202F;=&#x202F;0.292). Only the interaction of <italic>time</italic> &#x002A; <italic>group</italic> had a practically significant effect on satisfaction (<italic>&#x03B7;<sup>2</sup></italic><sub>p</sub>&#x202F;=&#x202F;0.21&#x2014;large effect), according to which satisfaction developed differently in the two groups over the 4&#x202F;years. While shift workers were significantly more satisfied with their available time at the follow-up (<italic>t</italic>(44)&#x202F;=&#x202F;6.36, <italic>p</italic>&#x202F;&#x003C; 0.001, <italic>d</italic>&#x202F;=&#x202F;0.94&#x2014;large effect), it did not change for day workers compared to the baseline measurement (<italic>t</italic>(29)&#x202F;=&#x202F;0.42, <italic>p&#x202F;=</italic> 0.675). In trend, shift workers were even more satisfied than day workers. The satisfaction score correlated only slightly (<italic>R</italic>&#x202F;=&#x202F;0.49) between baseline and follow-up. There was no significant effect on satisfaction for the covariate <italic>children/household [number]</italic> (<italic>p</italic> = 0.693). For the follow-up measurement, there was only low correlations with the satisfaction score for this covariate (<italic>children/household [number]: R</italic> = 0.21).</p>
<p>The satisfaction effect for shift workers was confirmed in the individual items: more shift than day workers stated that they were satisfied with the time available for sleep (SW: 88%, DW: 80%, <italic>d</italic>&#x202F;=&#x202F;0.64&#x2014;medium effect) as well as participation in organisations or club life (SW: 64%, DW: 30%, <italic>d</italic>&#x202F;=&#x202F;0.73&#x2014;medium effect). Satisfaction was comparable in both groups with regard to the time available for the family (81%, <italic>p</italic>&#x202F;=&#x202F;0.126) or partner (75%, <italic>p</italic>&#x202F;=&#x202F;0.520). Satisfaction with the time available for cultural events (49%, <italic>p</italic>&#x202F;=&#x202F;0.468), for hobbies and sporting activities (61%, <italic>p</italic>&#x202F;=&#x202F;0.062), as well as excursions and travel (59%, <italic>p</italic>&#x202F;=&#x202F;0.437) also did not differ between shift and day workers. However, only 31% of shift workers were still satisfied with their time for friends and relatives (DW: 53%, <italic>p</italic>&#x202F;=&#x202F;0.719).</p>
<p>For the impairment score, there was neither a significant main effect for <italic>group</italic> (<italic>p</italic>&#x202F;=&#x202F;0.436) nor an interaction effect for <italic>time &#x002A; group</italic> (<italic>p</italic>&#x202F;=&#x202F;0.526) but for the factor <italic>time</italic> (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.24&#x2014;large effect). Accordingly, comparable changes can be assumed in both groups over the period of 4&#x202F;years (<italic>t</italic>(74)&#x202F;=&#x202F;1.05, <italic>p</italic>&#x202F;=&#x202F;0.299). The changes at the follow-up indicated significantly less impairment in both shift and day workers (<italic>M</italic>&#x202F;=&#x202F;21 of 25 pts, SW: <italic>t</italic>(44)&#x202F;=&#x202F;4.46, <italic>d</italic>&#x202F;=&#x202F;0.68&#x2014;medium effect, DW: <italic>t</italic>(29)&#x202F;=&#x202F;3.19, <italic>d</italic>&#x202F;=&#x202F;0.58&#x2014;medium effect). A medium correlation (<italic>R</italic>&#x202F;=&#x202F;0.52) was found between baseline and follow-up for the impairment score. The <italic>number of children to be cared in the household</italic> had an influence on the impairments (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.07&#x2014;medium effect). MPOs without children reported work-related or private impairments more frequently than those with at least one child. There was a low negative correlation with the covariate <italic>children/household [number]</italic> (<italic>R</italic>&#x202F;=&#x202F;&#x2212;0.30). The individual items confirmed the clear decrease in work-related impairments in both groups.</p>
</sec>
</sec>
</sec>
<sec sec-type="discussion" id="sec32">
<label>4</label>
<title>Discussion</title>
<p>From an occupational health perspective, shift workers should be productive, resilient and satisfied with their work for as long as possible. Based on previous research findings, the study hypothesised at the beginning that MPOs working 12-h shifts over a period of 4&#x202F;years would experience negative effects on their sleep quality and quantity (<xref ref-type="bibr" rid="ref72">72</xref>, <xref ref-type="bibr" rid="ref73">73</xref>), cardiovascular health (<xref ref-type="bibr" rid="ref74">74</xref>) and work-life balance (<xref ref-type="bibr" rid="ref7">7</xref>). MPOs who worked daytime jobs served as a comparison group.</p>
<p>However, shift workers differed from day workers at the baseline measurement in terms of significantly poorer sleep quality, shorter sleep duration, less favourable values for blood pressure, body mass index, HDL cholesterol, PROCAM score and greater dissatisfaction with the time available for social, cultural and sporting activities.</p>
<p>In fact, the cardiovascular health status had not changed significantly in either group at the follow-up. For shift workers, the quality and quantity of sleep only decreased significantly after the night shift over a period of 4&#x202F;years. Nevertheless, most shift workers (T5: 89%) were satisfied with the time they had available for sleep. For day workers, the comparatively higher sleep quality and longer sleep duration from the baseline measurement were also confirmed in the follow-up measurement. The effect on satisfaction was unexpected. Shift workers were significantly more satisfied with their available free time in the follow-up. This satisfaction was now even higher than that of day workers, for whom satisfaction was comparable at both times (T1, T5). In addition, both groups reported significantly fewer impairment<italic>s</italic> after 4&#x202F;years, whereby this effect was moderated by the <italic>number of children to be cared for in the household</italic>. <italic>Age</italic> did not have a significant effect on sleep quality and quantity at any measurement <italic>time</italic> or in any <italic>group</italic>. However, with the exception of the lipid metabolism indicators, the known age effects were confirmed for the cardiovascular health indicators (BP, BMI, HbA1c, PROCAM score).</p>
<p>Shift workers slept on average 72&#x202F;min less than day workers at baseline, whereby their average sleep duration after the night shift was still 30&#x202F;min shorter than during the morning shift. For the follow-up, shift workers slept an average of only 5&#x202F;h 18&#x202F;min after the night shift, i.e., definitely too little. Daytime sleep after a night shift is also considered less restorative as it is more prone to disruption and rarely reaches the depth of night-time sleep (<xref ref-type="bibr" rid="ref8">8</xref>, <xref ref-type="bibr" rid="ref43">43</xref>). According to Hulsegge et al. (<xref ref-type="bibr" rid="ref17">17</xref>), around a third (35%) of shift workers and a quarter (27%) of day workers sleep less than seven hours. A representative survey of working people in Germany (<italic>n</italic>&#x202F;=&#x202F;4,511, 31&#x2013;60&#x202F;years) also found that 12% of shift workers can be assumed to have insufficient rest (<xref ref-type="bibr" rid="ref75">75</xref>). Research has shown that a lack of rest can lead to sleep disorders in the long term (<xref ref-type="bibr" rid="ref75">75</xref>). Health experts therefore recommend that shift workers who sleep less than 7&#x202F;h a day should take a power nap in between (<xref ref-type="bibr" rid="ref13">13</xref>).</p>
<p>Although the additional days off outside the 12-h shifts offer employees opportunities to compensate for sleep deficits and lack of rest, they are probably not enough to fully compensate for them. In the study by Cunha et al. (<xref ref-type="bibr" rid="ref8">8</xref>), only just under half (49%) of shift workers could imagine working in a 12-h shift system until retirement age. In addition, shift work appears to lead to a decrease in sleep quality after a short period of time (<xref ref-type="bibr" rid="ref73">73</xref>).</p>
<p>Sleep disorders are also widespread in the general population. In Germany, 29% of all men (18&#x2013;80&#x202F;years) (<xref ref-type="bibr" rid="ref57">57</xref>), and in an Austrian study 27% of all men (16-&#x202F;&#x2265;&#x202F;70&#x202F;years) have reported poor sleep quality (PSQI score &#x003E;5 pts) (<xref ref-type="bibr" rid="ref76">76</xref>). Chronic sleep disorders (PSQI score &#x003E;10 pts) were not reported by Zeitlhofer et al. (<xref ref-type="bibr" rid="ref76">76</xref>) and by Hinz et al. (<xref ref-type="bibr" rid="ref57">57</xref>) for 5% of men.</p>
<p>In the study by Hinz et al. (<xref ref-type="bibr" rid="ref57">57</xref>), the mean PSQI score for men in the general German population (18&#x2013;59&#x202F;years) was 4.3 pts In contrast to our sample, there was an age effect, according to which the PSQI score increased slightly with increasing age (&#x003C;40&#x202F;years: M&#x202F;=&#x202F;3.9 pts, 50&#x2013;59&#x202F;years: <italic>M</italic>&#x202F;=&#x202F;4.5 pts). In the study by Zeitlhofer et al. (<xref ref-type="bibr" rid="ref76">76</xref>), the PSQI score also increased with increasing age in men from 2.5 (19&#x2013;24&#x202F;years) to 5.5 pts (55&#x2013;59&#x202F;years). Compared to these two studies, the day workers in the present study showed significantly better sleep quality with an average of 3.5 pts at baseline and 3.1 pts at follow-up. In contrast, shift workers reported slightly worse PSQI scores at the follow-up (T5: <italic>M</italic>&#x202F;=&#x202F;4.8&#x2013;5.1 pts) than in the general German population (<xref ref-type="bibr" rid="ref57">57</xref>).</p>
<p>The correlations to the PSQI score between baseline and follow-up (SW: <italic>R</italic>&#x202F;=&#x202F;0.49, DW: <italic>R</italic>&#x202F;=&#x202F;0.60) and between morning and night shift (T1: <italic>R</italic>&#x202F;=&#x202F;0.63, T5: <italic>R</italic>&#x202F;=&#x202F;0.72) correspond to a variance explanation of 24&#x2013;52%. Accordingly, only those who reported good sleep quality at baseline tended to also report this at follow-up. This also applies to the correlation between morning and night shift. Obviously, sleep behaviour is based on &#x2018;third variables&#x2019; that occur independently of working hours. Sleep is a multidimensional construct and the causes of sleep problems are manifold.</p>
<p>With regard to cardiovascular health, the 12-h shift system had hardly any effect over a period of 4&#x202F;years in this study. On the contrary, significant age effects were found. It is assumed that shift workers develop strategies to maintain their health and well-being that prepare the body for 12-h shifts or compensate for the effects of working past 12&#x202F;h (<xref ref-type="bibr" rid="ref8">8</xref>). To categorise the health status of shift and day workers, comparative studies that represent the general German male population and approximately the age range of the study have been used; they are as follows: German Health Interview and Examination Survey for Adults (DEGS1 study, 18&#x2013;59 or 18-64&#x202F;years) (<xref ref-type="bibr" rid="ref77 ref78 ref79 ref80">77&#x2013;80</xref>), PROCAM study (<italic>n</italic>&#x202F;=&#x202F;5,389 men, average age: 47&#x202F;years) (<xref ref-type="bibr" rid="ref66">66</xref>) and Diabetes Cardiovascular Risk-Evaluation: Targets and Essential Data for Commitment of Treatment (DETECT study, <italic>n</italic>&#x202F;=&#x202F;3,672 men, 40&#x2013;65&#x202F;years, average age: 53&#x202F;years) (<xref ref-type="bibr" rid="ref81">81</xref>).</p>
<p>From a preventive point of view, there is a need for action not only for shift workers but also for day workers with regard to cardiovascular risk. For example, almost two thirds (62%) of shift workers and 40% of day workers were considered to be hypertensive at the time of the follow-up, of whom only 43 and 13%, respectively, were taking antihypertensive drugs. The decrease in average blood pressure observed over the four-year period among shift workers is probably a medication effect. In future, occupational health programmes need to focus on increasing the proportion of people with hypertension treated with medication, regardless of whether they work only during the day or in shifts.</p>
<p>Furthermore, about half of the shift workers (47%) and day workers (57%) were found to be overweight and 33 and 10%, respectively, were obese. In addition, lipid metabolism disorders were present in both groups. Although the risk of a heart attack in the next 10&#x202F;years could be classified as <italic>low</italic> for the majority of MPOs (T1:T5: &#x003C;10%: 84 vs. 82%, DW: 90%), it had increased in both groups for the follow-up (<italic>M</italic>&#x202F;=&#x202F;6.3 vs. 5.1 pts) although this was not practically significant. After 4&#x202F;years, 4% of MPOs had a high PROCAM risk (T1: 1%).</p>
<p>In the study by Ohlander et al. (<xref ref-type="bibr" rid="ref82">82</xref>), day workers had a lower prevalence of high blood pressure (8%) than shift workers (without night shift: 12%, night shift workers: 11%, rotating shift workers with nights: 10%). However, these prevalences are significantly lower than in our study and also lower than in the DEGS1 study, in which 15% of men had hypertension (<xref ref-type="bibr" rid="ref77">77</xref>). The low prevalences are explained by the healthy worker effect and higher medical monitoring of employees compared to the general German population (<xref ref-type="bibr" rid="ref82">82</xref>). Rashnuodi et al. (<xref ref-type="bibr" rid="ref31">31</xref>), on the other hand, found elevated blood pressure in 44% of shift workers and 28% of day workers. Esquirol et al. (<xref ref-type="bibr" rid="ref2">2</xref>) and Boini et al. (<xref ref-type="bibr" rid="ref35">35</xref>) also postulate an increased risk of hypertension for shift workers, although the duration of exposure could influence this correlation. According to Su et al. (<xref ref-type="bibr" rid="ref83">83</xref>), 12-h night work leads to an increase in blood pressure and heart rate as well as delayed blood pressure recovery. No association between shift work and high blood pressure was found in the longitudinal studies by Morikawa et al. (<xref ref-type="bibr" rid="ref32">32</xref>), Hublin et al. (<xref ref-type="bibr" rid="ref24">24</xref>) or Akbari et al. (<xref ref-type="bibr" rid="ref28">28</xref>).</p>
<p>The average body mass index in this study is comparable to the mean values of Ohlander et al. (<xref ref-type="bibr" rid="ref82">82</xref>) for both shift and day workers (SW with night work: <italic>M</italic>&#x202F;=&#x202F;28&#x202F;kg/m<sup>2</sup>, DW: <italic>M</italic>&#x202F;=&#x202F;26&#x202F;kg/m<sup>2</sup>). But the proportion of obese people among shift workers is significantly lower here (22&#x2013;24%) than among our shift workers (33%), but slightly higher for day workers (12% vs. 10%). In contrast, only 20% of men in the DEGS1 study were affected by obesity (<xref ref-type="bibr" rid="ref78">78</xref>). Results from reviews (<xref ref-type="bibr" rid="ref34">34</xref>, <xref ref-type="bibr" rid="ref35">35</xref>, <xref ref-type="bibr" rid="ref84">84</xref>) and the longitudinal study by van Drongelen et al. (<xref ref-type="bibr" rid="ref33">33</xref>) have confirmed that night work in particular is associated with an increased risk of being overweight and obesity. However, when the results were adjusted for potential confounders (e.g., age, physical activity), the evidence was insufficient (<xref ref-type="bibr" rid="ref33">33</xref>). In the cohort studies by Dochi et al. (<xref ref-type="bibr" rid="ref37">37</xref>) and Akbari et al. (<xref ref-type="bibr" rid="ref28">28</xref>), the mean values for the body mass index were in the normal range (&#x003C;25&#x202F;kg/m<sup>2</sup>) and did not differ between shift and day workers. This result was also reported by Rashnuodi et al. (<xref ref-type="bibr" rid="ref31">31</xref>), whereby the mean values of the body mass index (28&#x202F;kg/m<sup>2</sup>) were in the overweight range. Consistent with these results, the prevalence of being overweight, but above all obesity, also increased with age among the men in the DEGS1 study (<xref ref-type="bibr" rid="ref78">78</xref>).</p>
<p>In this study, the average increase in blood lipids occurred equally in both groups and could not be attributed to shift work, but rather to an age effect. There is consensus here with the results of Morikawa et al. (<xref ref-type="bibr" rid="ref32">32</xref>), Dochi et al. (<xref ref-type="bibr" rid="ref37">37</xref>), Akbari et al. (<xref ref-type="bibr" rid="ref28">28</xref>) and Dong et al. (<xref ref-type="bibr" rid="ref30">30</xref>), who also found no differences in lipid metabolism indicators between shift workers with night work and day work. In contrast, lipid metabolism disorders have been frequently found in reviews for shift and night workers (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref28">28</xref>, <xref ref-type="bibr" rid="ref31">31</xref>, <xref ref-type="bibr" rid="ref37">37</xref>). Compared to the DEGS1 study (TC: <italic>M</italic>&#x202F;=&#x202F;5.0&#x202F;mmol/l, HDL-C: <italic>M</italic>&#x202F;=&#x202F;1.3&#x202F;mmol/l), the MPOs showed slightly higher mean values for total cholesterol (T1:T5: <italic>M</italic>&#x202F;=&#x202F;5.3 vs. 5.5&#x202F;mmol/l), for HDL cholesterol the mean values were comparable to those of the DEGS1 study (1.3&#x202F;mmol/l) (<xref ref-type="bibr" rid="ref80">80</xref>) and the PROCAM study (1.2&#x202F;mmol/l) (<xref ref-type="bibr" rid="ref66">66</xref>). Overall, 51% of those analysed in the DEGS1 study had borderline elevated levels of total cholesterol, 16% had high levels of total cholesterol and 21% had low levels of HDL cholesterol (<xref ref-type="bibr" rid="ref80">80</xref>). In contrast, the sample shown here indicates a less favourable lipid metabolism status (T5: 31% elevated and 33% high TC values, 23% low HDL-C values). Boini et al. (<xref ref-type="bibr" rid="ref35">35</xref>) found low HDL cholesterol in permanent night and rotating night shift workers, while Guo et al. (<xref ref-type="bibr" rid="ref15">15</xref>) found no association between shift work and low HDL cholesterol. However, the link between lipid metabolism disorders and night shift work has not yet been clearly established (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref25">25</xref>).</p>
<p>In terms of glucose metabolism, shift and day workers did not differ longitudinally; 12-h shift work did not have a negative effect on the HbA1c value over 4&#x202F;years. On the contrary, the HbA1c value also increased with increasing age (T1-T5: <italic>R</italic>&#x202F;=&#x202F;0.45&#x2013;0.32). This finding contradicts the studies in which a higher prevalence of diabetes is postulated for shift and night workers compared to day workers (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref38">38</xref>, <xref ref-type="bibr" rid="ref85">85</xref>). In contrast, results from the studies by Morikawa et al. (<xref ref-type="bibr" rid="ref32">32</xref>), Akbari et al. (<xref ref-type="bibr" rid="ref28">28</xref>) or Dong et al. (<xref ref-type="bibr" rid="ref30">30</xref>), in which no differences were found between shift and day workers with regard to glucose metabolism, are consistent with the results given here. Unexpected was the decrease in the average HbA1c value for follow-up measurement in both groups. One possible explanation for this could be that the dietary habits of those affected had changed as a result of occupational health counselling.</p>
<p>Nevertheless, it is worth noting that 31% of shift workers and 23% of day workers had HbA1c values in the prediabetic range for the follow-up and 7% of all MPOs were affected by manifest diabetes. The prevalence of diabetes was therefore higher than in a comparable study with 12-h day and night shifts (<xref ref-type="bibr" rid="ref85">85</xref>). Here, 5% of alternating shift workers (n&#x202F;=&#x202F;4,150, average age: 45&#x202F;years) and 3% of day workers (n&#x202F;=&#x202F;5,976, average age: 44&#x202F;years) reported diagnosed diabetes mellitus. After additional adjustment of the diabetes risk factor (Find-Risk-Score), the prevalence of diabetes was on average 23% higher for shift workers compared to day workers. However, this effect was not significant and was primarily attributed to the more pronounced Find-Risk risk profile of shift workers (<xref ref-type="bibr" rid="ref85">85</xref>). The DEGS1 study found a diabetes prevalence of 3% for men (<xref ref-type="bibr" rid="ref79">79</xref>). In both studies, the prevalence increased with increasing age (<xref ref-type="bibr" rid="ref79">79</xref>, <xref ref-type="bibr" rid="ref85">85</xref>).</p>
<p>The PROCAM score used to summarise the assessment of cardiovascular health. The calculated risk of a cardiovascular event in the next 10&#x202F;years differed significantly between the groups (<italic>&#x03B7;</italic><sup>2</sup><sub>p</sub>&#x202F;=&#x202F;0.08&#x2014;medium effect) and increased with age. The age effect is also consistent with the findings from comparative studies (<xref ref-type="bibr" rid="ref15">15</xref>, <xref ref-type="bibr" rid="ref66">66</xref>, <xref ref-type="bibr" rid="ref79">79</xref>). The average PROCAM score for the follow-up was 6.3% for shift workers and 5.1% for day workers, which is still in the low-moderate range (<xref ref-type="bibr" rid="ref66">66</xref>). In the PROCAM study, an average PROCAM risk of 6.8% was determined (<xref ref-type="bibr" rid="ref66">66</xref>). In comparison, a significantly higher mean value of 9.2% was determined for the PROCAM score in the DETECT study (<xref ref-type="bibr" rid="ref81">81</xref>).</p>
<p>Our findings are consistent with those of the meta-analysis by Vyas et al. (<xref ref-type="bibr" rid="ref19">19</xref>), according to which shift workers have a higher risk of cardiovascular disease than day workers. However, the moderate increase in PROCAM risk for the follow-up is interpreted more as an age effect and less as a shift system effect, i.e., the fully continuous 12-h shift system does not appear to have an effect on cardiovascular risk. In the longitudinal study by Guo et al. (<xref ref-type="bibr" rid="ref15">15</xref>), no clear tendencies for an increased cardiovascular risk among shift workers were recognisable for men over a period of 22&#x202F;years. Yong et al. (<xref ref-type="bibr" rid="ref26">26</xref>) also found no evidence of an increased cardiovascular risk among 12-h shift workers in industrial production.</p>
<p>From a medical point of view, it is worrying that a significant proportion of the cardiovascular risks in the MPOs were only diagnosed as a result of the study. Most of them were unaware of their risk factors or diseases. As a result, they were not receiving medical treatment. This is where the potential lies for holistic occupational health care in Germany, which takes into account not only the working conditions but also the individual interactions between work and physical and mental health, which can jeopardise employability.</p>
<p>The predictability and plannability of working hours are the key to work-life balance. The 12-h shift system means that shift workers are exposed to work-related stress for longer, but have more continuous time for relaxation, family and non-work activities (<xref ref-type="bibr" rid="ref8">8</xref>). In addition, there is the financial advantage of shift allowances, less travelling time and costs, and free time at unusual times of the day (<xref ref-type="bibr" rid="ref8">8</xref>).</p>
<p>Satisfaction with the time available for social and leisure activities changed significantly differently in the groups between the two survey dates. While the shift workers were more dissatisfied at the baseline measurement, a positive satisfaction effect was registered over the four-year period. In addition, private life was hardly affected by shift work. Accordingly, the decision in favour of a 12-h shift schedule can be useful for shift workers at certain stages of life (e.g., with young children), while health aspects are of secondary importance in this context (<xref ref-type="bibr" rid="ref8">8</xref>). On the other hand, shift workers felt the sleep-related effects and occupational wear and tear of a shift system with night work. Irrespective of this, the benefits of the additional days off of a 12-h shift system described by Berkam et al. (<xref ref-type="bibr" rid="ref86">86</xref>) appear to contribute significantly to the satisfaction of shift workers. Overall, the findings point to a very diverse family and social private life for shift workers.</p>
<p>The family and social conditions of the shift and day workers were comparable at both measurement times: just under 80% of MPOs lived in a partnership. Around half of them were childless and only 8 and 10% of the shift workers had children up to the age of three in their household, so that hardly any adverse effects were expected.</p>
<p>In essence, the development of cardiovascular health over the course of a lifetime must be assumed to be multi-causal. The underlying mechanisms for the health consequences of shift work are not yet fully understood (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref87">87</xref>). So far, no causal relationship between shift work, disturbed sleep and health risks has been proven (<xref ref-type="bibr" rid="ref16">16</xref>, <xref ref-type="bibr" rid="ref25">25</xref>), even if numerous studies suggest this relationship. It is evident that general resilience decreases and illnesses increase with age.</p>
<p>The influence of shift work on cardiovascular and metabolic diseases is most frequently explained by desynchronized circadian rhythms, sleep disturbances, unhealthy diet, psychosocial work stress and social inequality (<xref ref-type="bibr" rid="ref2">2</xref>, <xref ref-type="bibr" rid="ref25">25</xref>). Although the body can adapt to changes in the daily routine in the short term, recurring disruptions to natural biological rhythms such as night work can lead to sleep disturbances and have health implications (including weight gain, increased lipids, coronary heart disease, type II diabetes) (<xref ref-type="bibr" rid="ref16">16</xref>, <xref ref-type="bibr" rid="ref19">19</xref>). The consumption of energy-dense foods during the night shift also appears to favour the development of dyslipidaemia and insulin resistance (<xref ref-type="bibr" rid="ref88">88</xref>). Lifestyle changes include unhealthier eating habits (<xref ref-type="bibr" rid="ref22">22</xref>, <xref ref-type="bibr" rid="ref25">25</xref>), smoking (<xref ref-type="bibr" rid="ref22">22</xref>, <xref ref-type="bibr" rid="ref35">35</xref>), reduced social (<xref ref-type="bibr" rid="ref43">43</xref>, <xref ref-type="bibr" rid="ref44">44</xref>) or physical activity (<xref ref-type="bibr" rid="ref22">22</xref>, <xref ref-type="bibr" rid="ref36">36</xref>, <xref ref-type="bibr" rid="ref40">40</xref>) have been discussed as further possible causes for the development of cardiovascular and metabolic diseases. In this study, more than half (57%) of the shift workers were regularly active in sports (DW: 80%) and one third (33%) were smokers (DW: 30%). In previous research, only a few studies have focused on measures to improve the health problems of shift workers. The current review by Wasiewicz-Ciach et al. (<xref ref-type="bibr" rid="ref13">13</xref>) summarises measures that shift workers can take to better protect their health.</p>
<p>In summary, the study showed that a 12-h rotating shift system is associated with poorer sleep quality and shorter sleep duration compared to working 8&#x202F;h during the day. Although these effects worsened over the four-year observation period, this only occurred after night shifts. This means that the hypothetical negative impact of a 12-h shift system on sleep can only be partially confirmed. During the same period, shift workers&#x2019; satisfaction with the 12-h shift system increased, reaching a level comparable to that of day workers. In addition, both groups reported a decrease in work-related impairments. These results lead to the rejection of the hypotheses regarding the negative impact of a 12-h shift system on work-life balance. In contrast, the cardiovascular health of the MPOs in both groups did not change over the observation period. This hypothesis must therefore also be rejected.</p>
<sec id="sec33">
<label>4.1</label>
<title>Strengths</title>
<p>The originality of the study consists in the fact that data of a homogenous occupational group in a clearly defined shift system were examined in a longitudinal design over 4&#x202F;years and possible covariates were checked. In addition, employees who work only during the day and whose occupations are largely comparable proved to be an important comparison group. Most of the studies on shift work have been based on a cross-sectional design with partially heterogeneous samples in which shift and night work are defined inconsistently and different shift systems are mixed. The studies have had different levels of evidence, in which covariables (e.g., nutrition, alcohol, smoking, exercise, sleep) have not been considered or in an undifferentiated way. Due to these limitations in the methodological quality of the studies, the conclusions drawn from them regarding the effects of shift work on sleep, health or work-life balance may not always have been justified and risks may have been over-interpreted.</p>
<p>In the present study, the shift work factor was added to the PSQI questionnaire and the retrospective period for recording sleep quality and quantity was again adapted to the original version &#x2013; it refers to the last 4&#x202F;weeks prior to the survey (<xref ref-type="bibr" rid="ref52">52</xref>). The German version of the PSQI (<xref ref-type="bibr" rid="ref56">56</xref>) was based on a period of only 2&#x202F;weeks, which limits the comparability with international studies.</p>
</sec>
<sec id="sec34">
<label>4.2</label>
<title>Limitations</title>
<p>It cannot be ruled out that the results are overlaid by different selection effects. For example, employees with good physical and mental adaptability (shift work tolerance) could remain in shift work with night work for longer than those who do not tolerate the stresses of shift work and night work or who have health problems (healthy worker effect, self-selection) (<xref ref-type="bibr" rid="ref43">43</xref>). Paradoxically, such effects can make shift workers appear healthier than &#x2018;normal day workers&#x2019; or the total population of the same age (<xref ref-type="bibr" rid="ref89">89</xref>). Since participation in the occupational health check-up was voluntary, it is possible that primarily those who were health-conscious and healthy took advantage of the examinations, i.e., the health risks of this sample could still have been underestimated.</p>
<p>The calculation of the sample size was based on a large effect size, which was not confirmed by the present results. Therefore, the study&#x2019;s power in detecting small differences in the outcomes between shift and day workers over time could be limited.</p>
<p>With regard to data collection, the results are subject to the well-known limitations of self-assessments using questionnaires (including distortions due to social desirability, response tendencies, memory deficits).</p>
<p>The body mass index is not accurate enough to determine individual health risks, as gender, age, origin, lifestyle, body composition, and fat and muscle mass are not included in its calculation. It can misestimate the health risk for overweight people and should therefore be supplemented by measures to estimate the fat distribution pattern in the body (waist-hip ratio or waist-to-height ratio). Regardless of this, the body mass index remains the decisive measure for diagnosing obesity in Germany (<xref ref-type="bibr" rid="ref64">64</xref>).</p>
<p>In order to use the DEGS1 studies as comparative data for the general German population, the age ranges for the present study were adjusted and recalculated. Exact comparisons require cross-calibrations of the measurement results, which are not available for pre-analytical conditions even with comparable measurement methods.</p>
<p>The present study is based on only one sample of 75 MPOs. Therefore, the results can only be generalised to other occupational groups that work in 12-h shifts to a limited extent.</p>
</sec>
</sec>
<sec sec-type="conclusions" id="sec35">
<label>5</label>
<title>Conclusion</title>
<p>The consequences of shift work are complex and depend on the specifics of the shift models. For acceptance among employees, it is crucial that shift work and non-work needs can be satisfactorily reconciled. In this respect, 12-h rotating shift systems seem to offer advantages over regular eight-hour&#x202F;day work. However, the shorter sleep duration and reduced sleep quality after night work are additional limitations that endanger the long-term employability of shift workers, especially older workers. To counteract this, workers must be offered sufficiently long rest periods after night work to compensate for sleep deficits.</p>
<p>There is no ideal shift model that can eliminate all the unfavourable effects of night work on employees. Night work is always accompanied by a desynchronization of the circadian and social rhythms. It is therefore necessary to find solutions that promote the health of shift workers. The 12-h shift system and the associated distribution of days off and work days affect the quality and quantity of sleep.</p>
<p>Since sleep disorders can reduce performance and quality of life and lead to long-term health problems, they must be detected and treated at an early stage. In this context, occupational medicine can provide a valuable supplement to care provided by general practitioners and specialists. Its task is to limit adverse effects of night shift work on sleep, health and work-life balance. Individual measures for better sleep hygiene could be just as beneficial as an effective company health management system with a focus on sleep and cardiovascular health in achieving a successful outcome in prevention.</p>
<p>The findings on cardiovascular health indicate a need for prevention and medical care for both shift and day workers. Monitoring the prevalence of hypertension, overweight and obesity would be relevant to health, but should be placed in the context of a healthy lifestyle.</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="sec36">
<title>Data availability statement</title>
<p>The datasets presented in this article are not readily available because the dataset supporting the conclusions of this article is not included in the article on the basis of agreed data protection commitments to the participants. Requests to access the datasets should be directed to <email>reingard.seibt@uni-rostock.de</email>.</p>
</sec>
<sec sec-type="ethics-statement" id="sec37">
<title>Ethics statement</title>
<p>The studies involving humans were approved by The Ethics Committee of the Technical University Dresden (EK 60042005). The studies were conducted in accordance with the local legislation and institutional requirements. The participants provided their written informed consent to participate in this study.</p>
</sec>
<sec sec-type="author-contributions" id="sec38">
<title>Author contributions</title>
<p>RS: Resources, Data curation, Visualization, Investigation, Methodology, Conceptualization, Validation, Funding acquisition, Supervision, Project administration, Software, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing, Formal analysis. SK: Supervision, Writing &#x2013; review &#x0026; editing, Visualization, Formal analysis, Validation, Resources. BH: Writing &#x2013; review &#x0026; editing, Conceptualization, Funding acquisition, Project administration.</p>
</sec>
<sec sec-type="funding-information" id="sec39">
<title>Funding</title>
<p>The author(s) declare that financial support was received for the research and/or publication of this article. This study was funded from the German Social Accident Insurance Institution for the foodstuffs and Catering Industry (BGN), Government Safety Organization Foods and Restaurants.</p>
</sec>
<ack>
<p>The authors would like to thank Thumedi Pr&#x00E4;ventionsmanagement GmbH, in particular Dorothee M&#x00F6;ller, who provided significant support in conducting the study, as well as all employees involved in the research project.</p>
</ack>
<sec sec-type="COI-statement" id="sec40">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="ai-statement" id="sec41">
<title>Generative AI statement</title>
<p>The author(s) declare that no Gen AI was used in the creation of this manuscript.</p>
</sec>
<sec sec-type="disclaimer" id="sec42">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
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</ref-list>
<glossary>
<title>Glossary</title>
<def-list list-type="simple" list-content="abbreviation">
<def-item>
<term id="G2">ArbZG</term>
<def>
<p>Working Hours Act</p>
</def>
</def-item>
<def-item>
<term id="G3">BafA</term>
<def>
<p>Federal Employment Agency</p>
</def>
</def-item>
<def-item>
<term id="G4">BGN</term>
<def>
<p>German Government Safety Organisation Foods and Restaurants</p>
</def>
</def-item>
<def-item>
<term id="G5">BMI</term>
<def>
<p>body mass index</p>
</def>
</def-item>
<def-item>
<term id="G6">BP</term>
<def>
<p>blood pressure</p>
</def>
</def-item>
<def-item>
<term id="G61">CI</term>
<def>
<p>confidence interval</p>
</def>
</def-item>
<def-item>
<term id="G7">DAG</term>
<def>
<p>German Obesity Society</p>
</def>
</def-item>
<def-item>
<term id="G8">DBP</term>
<def>
<p>diastolic blood pressure</p>
</def>
</def-item>
<def-item>
<term id="G9">DEGS1</term>
<def>
<p>German Health Interview and Examination Survey for Adults</p>
</def>
</def-item>
<def-item>
<term id="G10">DETECT</term>
<def>
<p>Diabetes Cardiovascular Risk-Evaluation: Targets and Essential Data for Commitment of Treatment</p>
</def>
</def-item>
<def-item>
<term id="G11">DW</term>
<def>
<p>day worker</p>
</def>
</def-item>
<def-item>
<term id="G12">HbA1c</term>
<def>
<p>glycated haemoglobin</p>
</def>
</def-item>
<def-item>
<term id="G13">HDL-C</term>
<def>
<p>high-density lipoprotein cholesterol</p>
</def>
</def-item>
<def-item>
<term id="G14">LDL-C</term>
<def>
<p>low-density lipoprotein cholesterol</p>
</def>
</def-item>
<def-item>
<term id="G15">MPO</term>
<def>
<p>machine and plant operators</p>
</def>
</def-item>
<def-item>
<term id="G151">OR</term>
<def>
<p>odds ratio</p>
</def>
</def-item>
<def-item>
<term id="G16">SBP</term>
<def>
<p>systolic blood pressure</p>
</def>
</def-item>
<def-item>
<term id="G17">PROCAM</term>
<def>
<p>Prospective Cardiovascular M&#x00FC;nster</p>
</def>
</def-item>
<def-item>
<term id="G18">PSQI</term>
<def>
<p>Pittsburgh Sleep Quality Index</p>
</def>
</def-item>
<def-item>
<term id="G19">SPSS</term>
<def>
<p>Statistical Package for the Social Science</p>
</def>
</def-item>
<def-item>
<term id="G20">SW</term>
<def>
<p>shift worker</p>
</def>
</def-item>
<def-item>
<term id="G21">TC</term>
<def>
<p>total cholesterol</p>
</def>
</def-item>
<def-item>
<term id="G22">TG</term>
<def>
<p>triglycerides</p>
</def>
</def-item>
</def-list>
</glossary>
</back>
</article>