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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Psychol.</journal-id>
<journal-title>Frontiers in Psychology</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Psychol.</abbrev-journal-title>
<issn pub-type="epub">1664-1078</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fpsyg.2024.1403146</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Psychology</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Optimism and mental health in college students: the mediating role of sleep quality and stress</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name><surname>Lai</surname> <given-names>Yun-Ju</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="corresp" rid="c001"><sup>&#x002A;</sup></xref>
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<contrib contrib-type="author">
<name><surname>Tsai</surname> <given-names>En-Yun</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
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<contrib contrib-type="author">
<name><surname>Jarustanaput</surname> <given-names>Ploypapus</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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</contrib>
<contrib contrib-type="author">
<name><surname>Wu</surname> <given-names>Yi-Syuan</given-names></name>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref>
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</contrib>
<contrib contrib-type="author">
<name><surname>Chen</surname> <given-names>Yi-Hau</given-names></name>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref>
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<contrib contrib-type="author">
<name><surname>O&#x2019;Leary</surname> <given-names>Samantha E.</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<contrib contrib-type="author">
<name><surname>Manachevakul</surname> <given-names>Sumatchara</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<contrib contrib-type="author">
<name><surname>Zhang</surname> <given-names>Yuan</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<contrib contrib-type="author">
<name><surname>Shen</surname> <given-names>Jiabin</given-names></name>
<xref ref-type="aff" rid="aff5"><sup>5</sup></xref>
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<contrib contrib-type="author">
<name><surname>Wang</surname> <given-names>Yan</given-names></name>
<xref ref-type="aff" rid="aff5"><sup>5</sup></xref>
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<aff id="aff1"><sup>1</sup><institution>School of Nursing, Zuckerberg College of Health Sciences, University of Massachusetts Lowell</institution>, <addr-line>Lowell, MA</addr-line>, <country>United States</country></aff>
<aff id="aff2"><sup>2</sup><institution>School of Pharmacy, College of Medicine, National Taiwan University</institution>, <addr-line>Taipei</addr-line>, <country>Taiwan</country></aff>
<aff id="aff3"><sup>3</sup><institution>Institute of Biomedical Sciences, Academia Sinica</institution>, <addr-line>Taipei</addr-line>, <country>Taiwan</country></aff>
<aff id="aff4"><sup>4</sup><institution>Institute of Statistical Science, Academia Sinica</institution>, <addr-line>Taipei</addr-line>, <country>Taiwan</country></aff>
<aff id="aff5"><sup>5</sup><institution>Department of Psychology, College of Fine Arts, Humanities &#x0026; Social Sciences, University of Massachusetts Lowell</institution>, <addr-line>Lowell, MA</addr-line>, <country>United States</country></aff>
<author-notes>
<fn fn-type="edited-by" id="fn0001">
<p>Edited by: Maura Pilotti, Prince Mohammad bin Fahd University, Saudi Arabia</p>
</fn>
<fn fn-type="edited-by" id="fn0002">
<p>Reviewed by: Maryam Bojulaia, Prince Mohammad bin Fahd University, Saudi Arabia</p>
<p>Lu&#x00ED;s S&#x00E9;rgio Vieira, University of Algarve, Portugal</p>
</fn>
<corresp id="c001">&#x002A;Correspondence: Yun-Ju Lai, <email>YunJu_Lai@uml.edu</email></corresp>
</author-notes>
<pub-date pub-type="epub">
<day>16</day>
<month>07</month>
<year>2024</year>
</pub-date>
<pub-date pub-type="collection">
<year>2024</year>
</pub-date>
<volume>15</volume>
<elocation-id>1403146</elocation-id>
<history>
<date date-type="received">
<day>18</day>
<month>03</month>
<year>2024</year>
</date>
<date date-type="accepted">
<day>25</day>
<month>06</month>
<year>2024</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2024 Lai, Tsai, Jarustanaput, Wu, Chen, O&#x2019;Leary, Manachevakul, Zhang, Shen and Wang.</copyright-statement>
<copyright-year>2024</copyright-year>
<copyright-holder>Lai, Tsai, Jarustanaput, Wu, Chen, O&#x2019;Leary, Manachevakul, Zhang, Shen and Wang</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<sec id="sec1">
<title>Objective</title>
<p>College students showed a high prevalence of stress, anxiety, and depression, with medical and nursing students experiencing particularly elevated levels of mental health challenges.</p>
<p>Optimism significantly influences overall well-being by promoting a healthy lifestyle and cognitive responses. However, the association of optimism with sleep quality, stress, and mental health in college students remains unexplored. This study aimed to (1) explore the associations of optimism with sleep quality, stress, and mental health and (2) ascertain whether sleep quality and stress mediate the association between optimism and mental health among college students.</p>
</sec>
<sec id="sec2">
<title>Methods</title>
<p>A cross-sectional study was conducted using online surveys with students from health science majors at a public university in the northeast United States from September to December 2022. A total of 222 students participated in the study, providing data on sociodemographics, optimism, sleep quality, stress, anxiety, and depression. Parallel and serial mediation models were utilized to examine the potential mediating roles of sleep quality and stress in the association between optimism and mental health.</p>
</sec>
<sec id="sec3">
<title>Results</title>
<p>The study found that optimism influences anxiety and depression through both direct and indirect pathways. In line with predictions, the parallel mediation analysis revealed that the impact of optimism on anxiety (&#x03B2;<sub>total</sub>&#x2009;=&#x2009;&#x2212;0.598, 95% confident interval [CI]: &#x2212;0.778 to &#x2212;0.392) and depression (&#x03B2;<sub>total</sub>&#x2009;=&#x2009;&#x2212;0.724, 95% CI: &#x2212;0.919 to &#x2212;0.519) was mediated by stress and sleep quality. Furthermore, the serial mediation models revealed that stress and sleep quality co-mediated the relationship betweenoptimism and anxiety (indirect effect [IE]&#x2009;=&#x2009;&#x2212;0.074, 95% CI: &#x2212;0.135 to &#x2212;0.029) or depression (IE&#x2009;=&#x2009;&#x2212;0.084, 95% CI: &#x2212;0.142 to &#x2212;0.036) in a sequential manner.</p>
</sec>
<sec id="sec4">
<title>Conclusion</title>
<p>Optimism was negatively correlated with poor sleep quality, stress, anxiety, and depression. Enhanced optimism was linked to high sleep quality and less stress, anxiety, and depression. These insights emphasize the potential for school-based optimism interventions to improve sleep quality, ameliorate stress-related concerns, and alleviate mental health challenges in college students.</p>
</sec>
</abstract>
<kwd-group>
<kwd>optimism</kwd>
<kwd>sleep quality</kwd>
<kwd>stress</kwd>
<kwd>anxiety</kwd>
<kwd>depression</kwd>
<kwd>mediation analysis</kwd>
</kwd-group>
<counts>
<fig-count count="3"/>
<table-count count="3"/>
<equation-count count="0"/>
<ref-count count="54"/>
<page-count count="8"/>
<word-count count="6555"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Positive Psychology</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec sec-type="intro" id="sec5">
<label>1</label>
<title>Introduction</title>
<p>Stress, anxiety, and depression are increasingly problematic in our society, with severe consequences for both physical and mental health (<xref ref-type="bibr" rid="ref6">Blanco et al., 2021</xref>). Beyond personal health implications, mental health problems impose a substantial economic burden on society, projected to reach around 16 trillion US dollars for the global economy by 2030 (<xref ref-type="bibr" rid="ref48">Trautmann et al., 2016</xref>; <xref ref-type="bibr" rid="ref34">Patel et al., 2018</xref>). College students are a particularly vulnerable population, exhibiting a higher prevalence of mental health issues compared to the general population (<xref ref-type="bibr" rid="ref31">Mofatteh, 2021</xref>). According to the National College Health Assessment from the American College Health Association, over 76% of undergraduates reported moderate or severe psychological distress (<xref ref-type="bibr" rid="ref2">American College Health Association, 2022</xref>). Furthermore, in the National Healthy Minds Study, 41% of college students disclosed their experience of major and moderate depression and 36% of them reported moderate or severe anxiety (<xref ref-type="bibr" rid="ref18">Healthy Minds Network, 2023</xref>). Poor mental health in college students may be associated with a range of problems, such as impaired academic performance, heavy alcohol consumption, substance abuse, low self-esteem, and suicide attempts (<xref ref-type="bibr" rid="ref20">Hysenbegasi et al., 2005</xref>; <xref ref-type="bibr" rid="ref44">Skidmore et al., 2016</xref>; <xref ref-type="bibr" rid="ref19">Hi&#x00E7;durmaz et al., 2017</xref>; <xref ref-type="bibr" rid="ref28">Liu et al., 2019</xref>; <xref ref-type="bibr" rid="ref39">S&#x00E6;ther et al., 2019</xref>). Given these costly outcomes for students, universities, and society, the mental health of undergraduate students is not only a crucial public health concern but also a pressing research priority.</p>
<p>College students&#x2019; poor mental health may stem from various challenges, including concerns regarding academic performance, pressure to succeed, and post-graduation plans (<xref ref-type="bibr" rid="ref5">Beiter et al., 2015</xref>). Particularly, students in health science fields such as medicine and nursing often grapple with heightened levels of anxiety and depression than other non-medical peers due to their heavy workload, including theoretical responsibilities and hands-on patient care (<xref ref-type="bibr" rid="ref31">Mofatteh, 2021</xref>). In addition, the quality of sleep has been closely linked to mental health issues, with poor sleep quality exacerbating the susceptibility of college nursing students to mental illnesses, including anxiety and depression (<xref ref-type="bibr" rid="ref52">Zhang et al., 2018</xref>). Students suffering from poor sleep quality often confronted high levels of perceived stress, which in turn precipitated anxiety or depression symptoms (<xref ref-type="bibr" rid="ref14">Doane et al., 2015</xref>; <xref ref-type="bibr" rid="ref52">Zhang et al., 2018</xref>), impaired psychosocial functioning (<xref ref-type="bibr" rid="ref46">Tavernier and Willoughby, 2014</xref>), and negatively impacted academic performance (<xref ref-type="bibr" rid="ref32">Okano et al., 2019</xref>). Furthermore, individuals with heightened stress were prone to develop concurrent anxiety (<xref ref-type="bibr" rid="ref17">Ghorbani et al., 2008</xref>), exhibit compromised sleep quality (<xref ref-type="bibr" rid="ref27">Liu et al., 2017</xref>), employ less healthy coping strategies (<xref ref-type="bibr" rid="ref15">Evans et al., 2015</xref>), and thus manifest depressive symptoms.</p>
<p>Optimism, defined as harboring positive expectations for the future (<xref ref-type="bibr" rid="ref41">Scheier and Carver, 1985</xref>), is a positive personality trait contributing to positive psychology (<xref ref-type="bibr" rid="ref13">Seligman and Csikszentmihalyi, 2014</xref>). Optimism is particularly vital during periods of uncertainty (<xref ref-type="bibr" rid="ref9">Carver et al., 1989</xref>), as demonstrated by its role as a protective factor against fear, stress, anxiety, and depression during the COVID-19 pandemic (<xref ref-type="bibr" rid="ref49">Vos et al., 2021</xref>). Heightened levels of optimism are associated with lower levels of anxiety and improved academic achievement among college students (<xref ref-type="bibr" rid="ref001">Singh and Jha, 2013</xref>), as well as better coping skills in response to stress (<xref ref-type="bibr" rid="ref002">Solberg Nes et al., 2009</xref>). Recent studies have demonstrated that optimism can promote positive emotions and higher life satisfaction, particularly during the COVID-19 pandemic (<xref ref-type="bibr" rid="ref30">Martinez et al., 2022</xref>). On the contrary, some studies suggested that lower levels of optimism and hope are aligned with decreased subjective well-being among young adults facing high levels of stress due to the pandemic (<xref ref-type="bibr" rid="ref16">Gen&#x00E7; and Arslan, 2021</xref>).</p>
<p>In the present, with the majority of studies focusing on the psychological health problems in college students, the mechanisms implicated in the links among optimism, sleep quality, stress, and mental health remain unclear. Therefore, this study aimed to <xref ref-type="bibr" rid="ref6">Blanco et al. (2021)</xref> explore the associations of optimism with sleep quality, stress, and mental health; and <xref ref-type="bibr" rid="ref48">Trautmann et al. (2016)</xref> ascertain whether sleep and stress mediate the connection between optimism and mental health, providing valuable insights into a promising intervention strategy regarding elevating optimism levels, thereby bolstering students&#x2019; mental well-being as they confront adversity.</p>
</sec>
<sec sec-type="materials|methods" id="sec6">
<label>2</label>
<title>Materials and methods</title>
<sec id="sec7">
<label>2.1</label>
<title>Study design and setting</title>
<p>This study was a quantitative cross-sectional study conducted among 222 undergraduate students in health science majors at a public university in the northeast United States in the fall of 2022. We employed a non-probability purposive sampling method to administer online surveys to all freshmen, sophomore, junior, and senior health science students.</p>
</sec>
<sec id="sec8">
<label>2.2</label>
<title>Inclusion and exclusion criteria</title>
<p>We included undergraduate students who were: <xref ref-type="bibr" rid="ref6">Blanco et al. (2021)</xref> aged 18&#x2009;years or older; <xref ref-type="bibr" rid="ref48">Trautmann et al. (2016)</xref> currently enrolled full-time; and <xref ref-type="bibr" rid="ref34">Patel et al. (2018)</xref> having access to the internet and capacity in computer typing. Students who were unable to provide informed consent were excluded.</p>
</sec>
<sec id="sec9">
<label>2.3</label>
<title>Sample size</title>
<p>A minimum correlation coefficient of 0.3 was assumed for variables including optimism, sleep quality, stress, and mental health, as supported by previous studies (<xref ref-type="bibr" rid="ref12">Cohen, 2013</xref>; <xref ref-type="bibr" rid="ref52">Zhang et al., 2018</xref>). With an aim to achieve 80% statistical power and a type I error rate of 0.05, a power analysis suggested a sample size of at least 67 participants for this study.</p>
</sec>
<sec id="sec10">
<label>2.4</label>
<title>Measurements</title>
<sec id="sec11">
<label>2.4.1</label>
<title>Revised life orientation test</title>
<p>The LOT-R was employed to measure the level of optimism (<xref ref-type="bibr" rid="ref42">Scheier et al., 1994</xref>). Consisting of 10 items, the LOT-R presents with 3 items, respectively, oriented in positive and negative directions, along with 4 filler items. Respondents are requested to indicate the extent to which they agree with each item on a 5-point Likert scale that ranges from strongly disagree to strongly agree. The total score is from 0 to 24, and the higher the LOT-R score, the higher the levels of optimism. The acceptable internal consistency of the LOT-R was reported as Cronbach&#x2019;s &#x03B1; of 0.82 (<xref ref-type="bibr" rid="ref43">Shifren and Anzaldi, 2018</xref>) in the stroke population, and stability (test&#x2013;retest reliability) over a 4-month period in college students was reported as <italic>r</italic>&#x2009;=&#x2009;0.79 (<xref ref-type="bibr" rid="ref42">Scheier et al., 1994</xref>). A scoring range of 0 to 13 points indicates a low level of optimism, whereas a range of 14 to 18 points suggests moderate optimism, and 19 to 24 points signifies a high level of optimism (<xref ref-type="bibr" rid="ref36">Przybyszowski et al., 2022</xref>). In this study, the scale demonstrated good reliability with a Cronbach&#x2019;s alpha of 0.79.</p>
</sec>
<sec id="sec12">
<label>2.4.2</label>
<title>Pittsburgh sleep quality index</title>
<p>The PSQI was used to assess the sleep quality in the previous month (<xref ref-type="bibr" rid="ref7">Buysse et al., 1989</xref>). Comprising 19 questions concerning sleep habits, the PSQI involves 7 components: sleep duration, sleep latency, sleep disturbance, daytime dysfunction, use of sleeping medications, habitual sleep efficiency, and overall sleep quality. This instrument has been previously validated for college students (<xref ref-type="bibr" rid="ref25">Lemma et al., 2014</xref>). Each component is scored on a scale of 0 to 3, yielding a global sleep quality score ranging from 0 to 21. Participants with PSQI scores exceeding 5 were identified as experiencing poor sleep quality (<xref ref-type="bibr" rid="ref7">Buysse et al., 1989</xref>). The PSQI in this study demonstrated good reliability with a Cronbach&#x2019;s alpha coefficient of 0.63.</p>
</sec>
<sec id="sec13">
<label>2.4.3</label>
<title>Perceived stress scale</title>
<p>The PSS is a 10-item questionnaire for evaluating the perception of stress during the past month (<xref ref-type="bibr" rid="ref11">Cohen et al., 1983</xref>). Participants were asked to rate the frequency of experiencing certain feelings and thoughts using a 5-point Likert scale. With 4 positively stated items reverse scored (e.g., 0&#x2009;=&#x2009;4, 1&#x2009;=&#x2009;3, 2&#x2009;=&#x2009;2, 3&#x2009;=&#x2009;1, and 4&#x2009;=&#x2009;0), the total PSS scores range from 0 to 40 and the higher PSS scores indicate the higher level of stress. The scale demonstrated good internal consistency (Cronbach&#x2019;s alpha) previously in undergraduate students (<xref ref-type="bibr" rid="ref26">Lin et al., 2020</xref>). The Cronbach&#x2019;s alpha in this study was 0.81.</p>
</sec>
<sec id="sec14">
<label>2.4.4</label>
<title>General anxiety disorder-7</title>
<p>The GAD-7 is a self-administered 7-item questionnaire, designed to assess the symptom severity of anxiety (<xref ref-type="bibr" rid="ref45">Spitzer et al., 2006</xref>). The GAD-7 is an acceptable questionnaire, with Cronbach&#x2019;s alpha of 0.89 in general populations (<xref ref-type="bibr" rid="ref29">Lowe et al., 2008</xref>). Each item is scored on a 4-point Likert-type scale, ranging from 0 to 3, and summed up with a final score ranging from 0 to 21. The scores of 5, 10, and 15 are cutoff points indicating mild, moderate, and severe levels of anxiety (<xref ref-type="bibr" rid="ref45">Spitzer et al., 2006</xref>). The GAD-7 scale demonstrated excellent reliability with a Cronbach&#x2019;s alpha of 0.92 for this study sample.</p>
</sec>
<sec id="sec15">
<label>2.4.5</label>
<title>Patient health questionnaire 9</title>
<p>The PHQ-9 is a self-administered questionnaire, employed to test the extent of depression (<xref ref-type="bibr" rid="ref22">Kroenke et al., 2001</xref>). The internal reliability of the PHQ-9 was demonstrated, with Cronbach&#x2019;s alpha of 0.81 to 0.84 (<xref ref-type="bibr" rid="ref23">Kroenke et al., 2016</xref>). Composed of 9 items, each rated on a 4-point Likert-type scale ranging from 0 to 3, the total scores on the PHQ-9 range from 0 to 27. Cutoff scores of 5, 10, 15, and 20 represent mild, moderate, moderately severe, and severe depression (<xref ref-type="bibr" rid="ref23">Kroenke et al., 2016</xref>). In this study, the scale demonstrated excellent reliability with a Cronbach&#x2019;s alpha of 0.90.</p>
</sec>
</sec>
<sec id="sec16">
<label>2.5</label>
<title>Data collection</title>
<p>Structured questionnaires comprising students&#x2019; sociodemographics (e.g., age, biological sex, race/ethnicity, study majors, grade levels, and history of psychotherapy, psychiatric medication, or psychiatric disorder), optimism (LOT-R), sleep quality (PSQI), stress (PSS), anxiety (GAD-7), and depression (PHQ-9), were administered through Qualtrics Online Surveys. Within the online survey, all the subjects were introduced to the study&#x2019;s purpose and procedures, potential risks and benefits, and assurance of privacy and confidentiality before proceeding to the survey sections. Additionally, embedded consent in the online survey required participants to agree prior to the initiation of the survey.</p>
</sec>
<sec id="sec17">
<label>2.6</label>
<title>Data analysis</title>
<p>Following the assessment of statistical normality, descriptive statistics, including the number of participants, age, biological sex, race/ethnicity, study majors, and grade levels, were reported as mean&#x2009;&#x00B1;&#x2009;SD or frequency (%). The Pearson correlation was applied to the relationships between sleep quality, stress, and mental health among college students. All the analyses were performed using SPSS 28.0 for Windows (SPSS Inc., Chicago, IL). Values of <italic>p</italic>&#x2009;&#x003C;&#x2009;0.05 were considered statistically significant. Mediation analysis, for both parallel and serial mediating effects of stress and sleep quality on the relationship between optimism and mental health, was performed using the package lavaan (<xref ref-type="bibr" rid="ref38">Rosseel, 2012</xref>), version 0.6.16, implemented in the R system for statistical computing (<xref ref-type="bibr" rid="ref37">Team RC, 2013</xref>). Mediation models were adjusted for the potential covariate, biological sex, to statistically control its effects and more accurately estimate the relationship between the predictors and the outcomes in the models.</p>
</sec>
</sec>
<sec sec-type="results" id="sec18">
<label>3</label>
<title>Results</title>
<sec id="sec19">
<label>3.1</label>
<title>Participant characteristics</title>
<p>A total of 222 students participated in the study, with a mean age of 20.3 (&#x00B1; 2.44) years. Predominately, respondents were female (81.5%), nursing (40.5%), sophomore (33.8%), and junior (31.1%) students. Almost 20% of the participants had a psychiatric history, with 59.5 and 50.0% of them experiencing anxiety and depression, respectively (<xref ref-type="table" rid="tab1">Table 1</xref>). <xref ref-type="fig" rid="fig1">Figure 1</xref> provides an overview of variable measurements within the respondent population. The LOT-R scores across the study population indicated a range of low optimism with a mean score of 13.12&#x2009;&#x00B1;&#x2009;3.99. It is noteworthy that 55.3% of the students were categorized as having low optimism, while 37.7% fell into the moderate optimism category (<xref ref-type="table" rid="tab2">Table 2</xref>). As outlined in <xref ref-type="table" rid="tab2">Table 2</xref> and <xref ref-type="fig" rid="fig1">Figure 1</xref>, participants reported experiencing various psychological states, including poor sleep quality, moderate stress, mild anxiety, and mild depression.</p>
<table-wrap position="float" id="tab1">
<label>Table 1</label>
<caption>
<p>Descriptive statistics for respondent demographic characteristics.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Characteristics</th>
<th align="center" valign="top">Subjects</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="middle">Age, year, Mean (S.D.)</td>
<td align="center" valign="middle">20.3 (2.44)</td>
</tr>
<tr>
<td align="left" valign="middle" colspan="2">Sex, n (%)</td>
</tr>
<tr>
<td align="left" valign="middle">Male</td>
<td align="center" valign="middle">41 (18.5)</td>
</tr>
<tr>
<td align="left" valign="middle">Female</td>
<td align="center" valign="middle">181 (81.5)</td>
</tr>
<tr>
<td align="left" valign="middle" colspan="2">Race/ethnicity, n (%)</td>
</tr>
<tr>
<td align="left" valign="middle">Non-Hispanic White or European-American</td>
<td align="center" valign="middle">97 (43.7)</td>
</tr>
<tr>
<td align="left" valign="middle">African American, Afro-Caribbean</td>
<td align="center" valign="middle">30 (13.5)</td>
</tr>
<tr>
<td align="left" valign="middle">Latino or Hispanic American</td>
<td align="center" valign="middle">43 (19.4)</td>
</tr>
<tr>
<td align="left" valign="middle">Asian and Asian American</td>
<td align="center" valign="middle">50 (22.5)</td>
</tr>
<tr>
<td align="left" valign="middle">Others</td>
<td align="center" valign="middle">2 (0.9)</td>
</tr>
<tr>
<td align="left" valign="middle" colspan="2">Study Major, n (%)</td>
</tr>
<tr>
<td align="left" valign="middle">Biomedical Sciences</td>
<td align="center" valign="middle">37 (16.7)</td>
</tr>
<tr>
<td align="left" valign="middle">Exercise Science</td>
<td align="center" valign="middle">29 (13.1)</td>
</tr>
<tr>
<td align="left" valign="middle">Nursing</td>
<td align="center" valign="middle">90 (40.5)</td>
</tr>
<tr>
<td align="left" valign="middle">Nutritional Science</td>
<td align="center" valign="middle">21 (9.5)</td>
</tr>
<tr>
<td align="left" valign="middle">Pharmaceutical Sciences</td>
<td align="center" valign="middle">6 (2.7)</td>
</tr>
<tr>
<td align="left" valign="middle">Public Health</td>
<td align="center" valign="middle">39 (17.6)</td>
</tr>
<tr>
<td align="left" valign="middle" colspan="2">Grade levels, n (%)</td>
</tr>
<tr>
<td align="left" valign="middle">Freshman</td>
<td align="center" valign="middle">36 (16.2)</td>
</tr>
<tr>
<td align="left" valign="middle">Sophomore</td>
<td align="center" valign="middle">75 (33.8)</td>
</tr>
<tr>
<td align="left" valign="middle">Junior</td>
<td align="center" valign="middle">69 (31.1)</td>
</tr>
<tr>
<td align="left" valign="middle">Senior</td>
<td align="center" valign="middle">41 (18.5)</td>
</tr>
<tr>
<td align="left" valign="middle">History of psychotherapy, yes, n (%)</td>
<td align="center" valign="middle">26 (11.7)</td>
</tr>
<tr>
<td align="left" valign="middle">History of psychiatric medication, yes, n (%)</td>
<td align="center" valign="middle">35 (15.8)</td>
</tr>
<tr>
<td align="left" valign="middle">History of psychiatric disorders, yes, n (%)</td>
<td align="center" valign="middle">42 (18.9)</td>
</tr>
<tr>
<td align="left" valign="middle">Anxiety</td>
<td align="center" valign="middle">25 (59.5)</td>
</tr>
<tr>
<td align="left" valign="middle">Depression</td>
<td align="center" valign="middle">21 (50.0)</td>
</tr>
<tr>
<td align="left" valign="middle">OCD</td>
<td align="center" valign="middle">2 (4.8)</td>
</tr>
<tr>
<td align="left" valign="middle">ADHD</td>
<td align="center" valign="middle">1 (2.4)</td>
</tr>
<tr>
<td align="left" valign="middle">Panic attack disorder</td>
<td align="center" valign="middle">1 (2.4)</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>(<italic>N</italic>&#x2009;=&#x2009;222). SD, Standard deviation; OCD, Obsessive-Compulsive Disorder; ADHD, Attention Deficit Hyperactivity Disorder.</p>
</table-wrap-foot>
</table-wrap>
<fig position="float" id="fig1">
<label>Figure 1</label>
<caption>
<p>The proportion of students across various severity levels in PHQ-9, GAD-7, PSS, PSQI, and LOT-R. The X-axis indicates the percentage of students, and the Y-axis denotes the variable measurements. LOT-R, revised Life Orientation Test; PSQI, Pittsburg Sleep Quality Index; PSS, Perceived Stress Scale; GAD-7, General Anxiety Disorder-7; PHQ-9, Patient Health Questionnaire-9.</p>
</caption>
<graphic xlink:href="fpsyg-15-1403146-g001.tif"/>
</fig>
<table-wrap position="float" id="tab2">
<label>Table 2</label>
<caption>
<p>Comparison of each variable measurements by biological sex.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Instruments</th>
<th align="left" valign="top">Categories</th>
<th align="center" valign="top">Total (<italic>n</italic>&#x2009;=&#x2009;222)</th>
<th align="center" valign="top">Male (<italic>n</italic>&#x2009;=&#x2009;41)</th>
<th align="center" valign="top">Female (<italic>n</italic>&#x2009;=&#x2009;181)</th>
<th align="center" valign="top">
<italic>p</italic>
</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" rowspan="4">LOT-R</td>
<td align="left" valign="top">Low</td>
<td align="center" valign="top">119 (55.3%)</td>
<td align="center" valign="top">18 (45.0%)</td>
<td align="center" valign="top">101 (57.7%)</td>
<td align="center" valign="top" rowspan="3">0.06<sup>a</sup></td>
</tr>
<tr>
<td align="left" valign="top">Moderate</td>
<td align="center" valign="top">81 (37.7%)</td>
<td align="center" valign="top">16 (40%)</td>
<td align="center" valign="top">65 (37.1%)</td>
</tr>
<tr>
<td align="left" valign="top">High</td>
<td align="center" valign="top">15 (7.0%)</td>
<td align="center" valign="top">6 (15.0%)</td>
<td align="center" valign="top">9 (5.1%)</td>
</tr>
<tr>
<td align="left" valign="top">Score Mean&#x2009;&#x00B1;&#x2009;S.D.</td>
<td align="center" valign="top">13.12&#x2009;&#x00B1;&#x2009;3.99</td>
<td align="center" valign="top">14.08&#x2009;&#x00B1;&#x2009;3.99</td>
<td align="center" valign="top">12.90&#x2009;&#x00B1;&#x2009;3.97</td>
<td align="center" valign="top">0.09<sup>b</sup></td>
</tr>
<tr>
<td align="left" valign="top" rowspan="3">PSQI</td>
<td align="left" valign="top">Good</td>
<td align="center" valign="top">64 (29.8%)</td>
<td align="center" valign="top">21 (52.5)</td>
<td align="center" valign="top">43 (24.6%)</td>
<td align="center" valign="top" rowspan="2">&#x003C;0.001<sup>a</sup></td>
</tr>
<tr>
<td align="left" valign="top">Poor</td>
<td align="center" valign="top">151 (70.2%)</td>
<td align="center" valign="top">19 (47.5)</td>
<td align="center" valign="top">132 (75.4%)</td>
</tr>
<tr>
<td align="left" valign="top">Score Mean&#x2009;&#x00B1;&#x2009;S.D.</td>
<td align="center" valign="top">7.66&#x2009;&#x00B1;&#x2009;3.31</td>
<td align="center" valign="top">6.15&#x2009;&#x00B1;&#x2009;3.17</td>
<td align="center" valign="top">8.00&#x2009;&#x00B1;&#x2009;3.25</td>
<td align="center" valign="top">0.002<sup>b</sup></td>
</tr>
<tr>
<td align="left" valign="top" rowspan="4">PSS</td>
<td align="left" valign="top">Low</td>
<td align="center" valign="top">33 (15.3%)</td>
<td align="center" valign="top">13 (34.2%)</td>
<td align="center" valign="top">20 (11.2%)</td>
<td align="center" valign="top" rowspan="3">0.001<sup>a</sup></td>
</tr>
<tr>
<td align="left" valign="top">Moderate</td>
<td align="center" valign="top">155 (71.8%)</td>
<td align="center" valign="top">23 (60.5%)</td>
<td align="center" valign="top">132 (74.2%)</td>
</tr>
<tr>
<td align="left" valign="top">High</td>
<td align="center" valign="top">28 (13.0%)</td>
<td align="center" valign="top">2 (5.3%)</td>
<td align="center" valign="top">26 (14.6%)</td>
</tr>
<tr>
<td align="left" valign="top">Score Mean&#x2009;&#x00B1;&#x2009;S.D.</td>
<td align="center" valign="top">19.93&#x2009;&#x00B1;&#x2009;5.86</td>
<td align="center" valign="top">16.55&#x2009;&#x00B1;&#x2009;6.55</td>
<td align="center" valign="top">20.65&#x2009;&#x00B1;&#x2009;5.45</td>
<td align="center" valign="top">&#x003C;0.001<sup>b</sup></td>
</tr>
<tr>
<td align="left" valign="top" rowspan="5">GAD-7</td>
<td align="left" valign="top">None</td>
<td align="center" valign="top">59 (26.8%)</td>
<td align="center" valign="top">21 (52.5%)</td>
<td align="center" valign="top">38 (21.1%)</td>
<td align="center" valign="top" rowspan="4">&#x003C;0.001<sup>a</sup></td>
</tr>
<tr>
<td align="left" valign="top">Mild</td>
<td align="center" valign="top">61 (27.7%)</td>
<td align="center" valign="top">10 (25.0%)</td>
<td align="center" valign="top">51 (28.3%)</td>
</tr>
<tr>
<td align="left" valign="top">Moderate</td>
<td align="center" valign="top">48 (21.8%)</td>
<td align="center" valign="top">3 (7.5%)</td>
<td align="center" valign="top">45 (25%)</td>
</tr>
<tr>
<td align="left" valign="top">Severe</td>
<td align="center" valign="top">52 (23.6%)</td>
<td align="center" valign="top">6 (15.0%)</td>
<td align="center" valign="top">46 (25.6%)</td>
</tr>
<tr>
<td align="left" valign="top">Score Mean&#x2009;&#x00B1;&#x2009;S.D.</td>
<td align="center" valign="top">9.25&#x2009;&#x00B1;&#x2009;6.10</td>
<td align="center" valign="top">5.85&#x2009;&#x00B1;&#x2009;5.97</td>
<td align="center" valign="top">10.00&#x2009;&#x00B1;&#x2009;5.88</td>
<td align="center" valign="top">&#x003C;0.001<sup>b</sup></td>
</tr>
<tr>
<td align="left" valign="top" rowspan="6">PHQ-9</td>
<td align="left" valign="top">None</td>
<td align="center" valign="top">80 (38.3%)</td>
<td align="center" valign="top">21 (56.8%)</td>
<td align="center" valign="top">59 (34.3%)</td>
<td align="center" valign="top" rowspan="5">0.059<sup>a</sup></td>
</tr>
<tr>
<td align="left" valign="top">Mild</td>
<td align="center" valign="top">53 (25.4%)</td>
<td align="center" valign="top">10 (27.0%)</td>
<td align="center" valign="top">43 (25.0%)</td>
</tr>
<tr>
<td align="left" valign="top">Moderate</td>
<td align="center" valign="top">28 (13.4%)</td>
<td align="center" valign="top">2 (5.4%)</td>
<td align="center" valign="top">26 (15.1%)</td>
</tr>
<tr>
<td align="left" valign="top">Moderately Severe</td>
<td align="center" valign="top">36 (17.2%)</td>
<td align="center" valign="top">3 (8.1%)</td>
<td align="center" valign="top">33 (19.2%)</td>
</tr>
<tr>
<td align="left" valign="top">Severe</td>
<td align="center" valign="top">12 (5.7%)</td>
<td align="center" valign="top">1 (2.7%)</td>
<td align="center" valign="top">11 (6.4%)</td>
</tr>
<tr>
<td align="left" valign="top">Score Mean&#x2009;&#x00B1;&#x2009;S.D.</td>
<td align="center" valign="top">8.29&#x2009;&#x00B1;&#x2009;6.55</td>
<td align="center" valign="top">5.51&#x2009;&#x00B1;&#x2009;5.48</td>
<td align="center" valign="top">8.88&#x2009;&#x00B1;&#x2009;6.63</td>
<td align="center" valign="top">0.002<sup>b</sup></td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>SD, Standard deviation; LOT-R, revised Life Orientation Test; PSQI, Pittsburg Sleep Quality Index; PSS, Perceived Stress Scale; GAD-7, General Anxiety Disorder-7; PHQ-9, Patient Health Questionnaire-9. The <italic>p</italic>-values were tested using two-way-ANOVA<sup>a</sup> or Mann-Whitney U test<sup>b</sup>.</p>
</table-wrap-foot>
</table-wrap>
<p>Upon a biological sex-based analysis, it was observed that male students exhibited notably better sleep quality, lower levels of stress, anxiety, and depression in comparison to female counterparts (<xref ref-type="table" rid="tab2">Table 2</xref>). However, there was no statistical difference observed in the optimism levels between male (14.08&#x2009;&#x00B1;&#x2009;3.99) and female students (12.90&#x2009;&#x00B1;&#x2009;3.97). In addition, no statistically significant differences were identified in the mean scores for LOT-R, PSQI, PSS, GAD-7 or PHQ-9 based on respondents&#x2019; ethnicity, study major and grade levels (Supplementary Tables 1&#x2013;3).</p>
</sec>
<sec id="sec20">
<label>3.2</label>
<title>Correlations of optimism with sleep quality, stress, and mental health among college students</title>
<p>As <xref ref-type="table" rid="tab3">Table 3</xref> shows, the college students&#x2019; optimism (LOT-R) demonstrated a significant negative correlation with poor sleep quality (<italic>r</italic>&#x2009;=&#x2009;&#x2212;0.281, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), stress (<italic>r</italic>&#x2009;=&#x2009;&#x2212;0.486, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), anxiety (<italic>r</italic>&#x2009;=&#x2009;&#x2212;0.423, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), and depression (<italic>r</italic>&#x2009;=&#x2009;&#x2212;0.476, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001). Notably, poor sleep quality (PSQI &#x003E;5) showed a positive correlation with stress (<italic>r</italic>&#x2009;=&#x2009;0.498, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), anxiety (<italic>r</italic>&#x2009;=&#x2009;0.488, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), and depression (<italic>r</italic>&#x2009;=&#x2009;0.499, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001). Moreover, elevated stress levels were linked to higher anxiety (<italic>r</italic>&#x2009;=&#x2009;0.675, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001) and depression (<italic>r</italic>&#x2009;=&#x2009;0.675, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001).</p>
<table-wrap position="float" id="tab3">
<label>Table 3</label>
<caption>
<p>The associations of optimism with sleep quality, stress, anxiety, and depression among college students (<italic>n</italic>&#x2009;=&#x2009;222).</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Variables</th>
<th align="center" valign="top">LOT-R</th>
<th align="center" valign="top">PSQI</th>
<th align="center" valign="top">PSS</th>
<th align="center" valign="top">GAD-7</th>
<th align="center" valign="top">PHQ-9</th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="middle">LOT-R</td>
<td align="center" valign="middle">1.000</td>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="middle">PSQI</td>
<td align="center" valign="middle">&#x2212;0.281&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">1.000</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="middle">PSS</td>
<td align="center" valign="middle">&#x2212;0.486&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">0.498&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">1.000</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="middle">GAD-7</td>
<td align="center" valign="middle">&#x2212;0.423&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">0.488&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">0.675&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">1.000</td>
<td/>
</tr>
<tr>
<td align="left" valign="middle">PHQ-9</td>
<td align="center" valign="middle">&#x2212;0.476&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">0.499&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">0.675&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">0.755&#x002A;&#x002A;&#x002A;</td>
<td align="center" valign="middle">1.000</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>LOT-R, revised Life Orientation Test; PSQI, Pittsburg Sleep Quality Index; PSS, Perceived Stress Scale; GAD-7, General Anxiety Disorder-7; PHQ-9, Patient Health Questionnaire 9. Pearson&#x2019;s Correlation, &#x002A;&#x002A;&#x002A;<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001.</p>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec21">
<label>3.3</label>
<title>The mediating role of stress and sleep quality</title>
<sec id="sec22">
<label>3.3.1</label>
<title>Parallel mediation models</title>
<p>To understand how optimism, directly and indirectly, influenced anxiety or depression, parallel mediation models were conducted. In <xref ref-type="fig" rid="fig2">Figure 2A</xref>, as the direct effect of optimism toward anxiety became insignificant (Direct Effect [DE]&#x2009;=&#x2009;&#x2212;0.174, <italic>p</italic>&#x2009;=&#x2009;0.060), stress and sleep quality fully mediated the association between optimism and anxiety after the adjustment for biological sex. Specifically, stress and sleep quality exhibited significant indirect effects on this association (Indirect Effect [IE]<sub>PSS</sub>&#x2009;=&#x2009;&#x2212;0.333, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001; IE<sub>PSQI</sub>&#x2009;=&#x2009;&#x2212;0.091, <italic>p</italic>&#x2009;=&#x2009;0.005). These findings indicated that students with enhanced optimism may experience lower anxiety levels through reduced stress and improved sleep quality. Besides, compared to sleep quality, lower perceived stress was the more dominant route on the impact of optimism on anxiety.</p>
<fig position="float" id="fig2">
<label>Figure 2</label>
<caption>
<p>Parallel mediation models for the mediating effects of stress and sleep quality on the relationship between optimism and mental health. Direct and indirect effects of optimism on <bold>(A)</bold> anxiety or <bold>(B)</bold> depression through stress and sleep quality are illustrated. The models are adjusted for biological sex. All effects presented are unstandardized. The direct effect and the (total effect) are depicted on the path directly from optimism to anxiety or depression. &#x002A;&#x002A;&#x002A;<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001. LOT-R, revised Life Orientation Test; PSQI, Pittsburg Sleep Quality Index; PSS, Perceived Stress Scale; GAD-7, General Anxiety Disorder-7; PHQ-9, Patient Health Questionnaire-9.</p>
</caption>
<graphic xlink:href="fpsyg-15-1403146-g002.tif"/>
</fig>
<p>Following the adjustment for biological sex and the introduction of stress and sleep quality into the Model 2 (<xref ref-type="fig" rid="fig2">Figure 2B</xref>), the attenuated path coefficient (DE&#x2009;=&#x2009;&#x2212;0.303, <italic>p</italic>&#x2009;=&#x2009;0.001) of optimism on depression revealed the partial mediating roles of stress and sleep quality in the optimism-depression relationship. Both stress and sleep quality demonstrated significant mediating effects on optimism with depression (IE<sub>PSS</sub>&#x2009;=&#x2009;&#x2212;0.327, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001; IE<sub>PSQI</sub>&#x2009;=&#x2009;&#x2212;0.095, <italic>p</italic>&#x2009;=&#x2009;0.015), reflecting the stronger mediating role of stress. Similar to the Model 1 (<xref ref-type="fig" rid="fig2">Figure 2A</xref>), students with higher optimism levels were less susceptible to depression through reduced stress and enhanced sleep quality. Additionally, the influence of optimism on depression appeared to be primarily channeled through lower perceived stress. Overall, the total effects derived from these two models revealed statistically significant negative associations between optimism and anxiety (&#x03B2;<sub>total</sub>&#x2009;=&#x2009;&#x2212;0.598, 95% CI: &#x2212;0.778 to &#x2212;0.392), and optimism and depression (&#x03B2;<sub>total</sub>&#x2009;=&#x2009;&#x2212;0.724, 95% CI: &#x2212;0.919 to &#x2212;0.519), which implied a more pronounced impact of optimism on depression compared to anxiety.</p>
</sec>
<sec id="sec23">
<label>3.3.2</label>
<title>Serial mediation models</title>
<p>To explore the mediating roles of stress and sleep quality linked in casual chains, guided by a specific directional flow, serial mediation models were employed. Since two mediators of stress and sleep quality were used and with two different outcome variables (anxiety and depression), a total of four different causal order models were produced, with only two of which were presented in <xref ref-type="fig" rid="fig3">Figure 3</xref>. Each distinct casual order of the mediators was examined to compare the significant paths generated by the four models. The total indirect effects derived from serial mediation models were all found to be statistically significant. Of note, the two mediators were shown to partially mediate in the relationship between optimism and anxiety, as well as in the optimism-depression link. This observation denotes a nuanced departure from the result derived from the parallel mediation models (<xref ref-type="fig" rid="fig2">Figure 2</xref>).</p>
<fig position="float" id="fig3">
<label>Figure 3</label>
<caption>
<p>Serial mediation models for the mediating effects of stress and sleep quality on the relationship between optimism and mental health. Four serial mediating pathways are illustrated, respectively, in the serial mediation models of <bold>(A)</bold> optimism to anxiety and <bold>(B)</bold> optimism to depression. The models are adjusted for biological sex. All effects presented are unstandardized. The direct effect and the (total effect) are depicted on the path directly from optimism to anxiety or depression. &#x002A;<italic>p</italic>&#x2009;&#x003C;&#x2009;0.05, &#x002A;&#x002A;&#x002A;<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001. LOT-R, revised Life Orientation Test; PSQI, Pittsburg Sleep Quality Index; PSS, Perceived Stress Scale; GAD-7, General Anxiety Disorder-7; PHQ-9, Patient Health Questionnaire-9.</p>
</caption>
<graphic xlink:href="fpsyg-15-1403146-g003.tif"/>
</fig>
<p>In the analysis of serial mediation models depicting the transition from optimism to anxiety, both the indicated indirect effect path (LOT-R/PSS/PSQI/GAD-7, IE&#x2009;=&#x2009;&#x2212;0.074, 95% CI: &#x2212;0.135 to &#x2212;0.029) presented in <xref ref-type="fig" rid="fig3">Figure 3A</xref> and the alternative indirect effect path (LOT-R/PSQI/PSS/GAD-7, IE&#x2009;=&#x2009;&#x2212;0.066, 95% CI: &#x2212;0.114 to &#x2212;0.032) demonstrated statistical significance. Additionally, within the serial mediation models that trace the impact of optimism on depression, the specified indirect pathway denoted as LOT-R/PSS/PSQI/PHQ-9 (<xref ref-type="fig" rid="fig3">Figure 3B</xref>, IE&#x2009;=&#x2009;&#x2212;0.084, 95% CI: &#x2212;0.142 to &#x2212;0.036) and the alternative pathway of LOT-R/PSQI/PSS/PHQ-9 (IE&#x2009;=&#x2009;&#x2212;0.064, 95% CI: &#x2212;0.114 to &#x2212;0.026) similarly exhibited significant mediating effect. Collectively, these findings imply that increased optimism would lead to lower perceived stress, subsequently improving sleep quality and ultimately contributing to reduced levels of anxiety and depression.</p>
</sec>
</sec>
</sec>
<sec sec-type="discussion" id="sec24">
<label>4</label>
<title>Discussion</title>
<p>This study explored the inter-relations between optimism and mental health in college students, with a specific aim to examine the mediating effects of sleep quality and stress in the relationship. Our findings revealed a significant negative correlation between optimism and poor sleep quality, stress, anxiety as well as depression. The result was consistent with the previous finding indicating that college students with higher scores on optimism reported improved sleep quality and lower levels of stress, anxiety, and depression, which might be targeted to reduce mental health problems and improve academic success (<xref ref-type="bibr" rid="ref10">Cheuk Yan and Wong, 2011</xref>; <xref ref-type="bibr" rid="ref21">Kim et al., 2022</xref>). Additionally, parallel mediation analysis demonstrated that stress and sleep quality fully mediated the relationship between optimism and anxiety, while partially mediating the optimism-depression link. Regarding serial mediation analysis, a significant mediating path sequentially followed by stress and sleep quality was demonstrated both in optimism-anxiety and optimism-depression models.</p>
<p>With the potential underlying mechanism inferred from parallel and serial mediation analyses, this study highlighted the importance of optimism as a mechanism through which reduced levels of stress and improved sleep quality can translate into anxiety and depression. Moreover, the findings also underlined the vulnerability of health science students, as they contend with a variety of academic and clinical stressors, including long hours of study, the demanding nature of examinations, and lack of free time (<xref ref-type="bibr" rid="ref33">Papazisis et al., 2008</xref>; <xref ref-type="bibr" rid="ref40">Sakai et al., 2022</xref>). Therefore, school-based interventions may hold promise in ameliorating students&#x2019; stress, improving their sleep quality, and further reducing the levels of anxiety, and depression.</p>
<p>Aside from examining the association between optimism and mental health among college students, this study also serves to confirm the degree of optimism, sleep quality, stress, anxiety, and depression experienced by college students in the post-COVID era. The emergence of the coronavirus disease 2019 (COVID-19) has brought forth not only physical health problems but also psychological issues (<xref ref-type="bibr" rid="ref47">Tran et al., 2020</xref>; <xref ref-type="bibr" rid="ref51">Xiong et al., 2020</xref>). College students, in particular, are adversely impacted owing to the uncertainty surrounding academic achievement, future career prospects, and social lives (<xref ref-type="bibr" rid="ref3">Aristovnik et al., 2021</xref>). The disruptions caused by school closures, cancelation of social events, remote online courses, and exam postponements during the COVID-19 pandemic heightened their emotional distress (<xref ref-type="bibr" rid="ref8">Cao et al., 2020</xref>; <xref ref-type="bibr" rid="ref24">Lei et al., 2020</xref>; <xref ref-type="bibr" rid="ref51">Xiong et al., 2020</xref>). However, the psychological challenges faced by students did not end with the remission of the pandemic and the easing of social restrictions. In this study, our participants reported low optimism, poor sleep quality, moderate stress, mild anxiety, and depression, reflecting the persisting psychological impact of the pandemic. Consistent with our findings, other study has also highlighted that students perceived intensified levels of stress and anxiety, as well as moderate depression after returning to campus (<xref ref-type="bibr" rid="ref1">Al-Rawi et al., 2022</xref>). Factors contributing to their psychological struggles include fear of infection and enduring social, family, and economic changes resulting from the COVID-19 pandemic (<xref ref-type="bibr" rid="ref50">Wang et al., 2022</xref>).</p>
<p>There are several limitations inherent in this study. First, the study sample was exclusively drawn from Health Sciences students at a single public university in the United States, thereby limiting the generalizability of the findings to broader populations or countries. Second, the biological sex composition of the participants was predominately female, and recent studies have shown biological sex-based differences in student mental health consequences of the COVID-19 pandemic (<xref ref-type="bibr" rid="ref4">Aus&#x00ED;n et al., 2021</xref>; <xref ref-type="bibr" rid="ref35">Prowse et al., 2021</xref>). Hence, this biological sex imbalance may limit the generalizability of the results to the male population. Third, the study lacked longitudinal follow-up and, therefore, did not demonstrate causal relationships, although the outcomes do imply that researchers, clinicians, and schools should take into account these variable interactions between optimism and mental health among college students.</p>
</sec>
<sec sec-type="conclusions" id="sec25">
<label>5</label>
<title>Conclusion</title>
<p>This study documented the direct and indirect effects of stress and sleep quality and its sequential mediating pathway in the connection between optimism and mental health within health science college students. Findings from the study underscore the significance of fostering academic optimism to alleviate stress and improve sleep quality, ultimately expecting to ease the mental health burdens experienced by college students. Consequently, the development of diverse academic programs focused on enhancing the optimism of college students becomes imperative.</p>
</sec>
<sec sec-type="data-availability" id="sec26">
<title>Data availability statement</title>
<p>The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.</p>
</sec>
<sec sec-type="ethics-statement" id="sec27">
<title>Ethics statement</title>
<p>The study involving human participants was reviewed and approved by the Institutional Review Board (IRB) at the University of Massachusetts Lowell (IRB# 22-072-LAI-EXM). The studies were conducted in accordance with the local legislation and institutional requirements. The participants provided their written informed consent to participate in this study.</p>
</sec>
<sec sec-type="author-contributions" id="sec28">
<title>Author contributions</title>
<p>Y-JL: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. E-YT: Resources, Visualization, Writing &#x2013; review &#x0026; editing. PJ: Formal analysis, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. Y-SW: Formal analysis, Methodology, Writing &#x2013; review &#x0026; editing. Y-HC: Formal analysis, Methodology, Writing &#x2013; review &#x0026; editing. SO&#x2019;L: Data curation, Writing &#x2013; review &#x0026; editing. SM: Data curation, Writing &#x2013; review &#x0026; editing. YZ: Conceptualization, Validation, Writing &#x2013; review &#x0026; editing. JS: Validation, Writing &#x2013; review &#x0026; editing. YW: Methodology, Validation, Writing &#x2013; review &#x0026; editing.</p>
</sec>
</body>
<back>
<sec sec-type="funding-information" id="sec29">
<title>Funding</title>
<p>The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was funded by the Donna Manning Pilot Research Program and the University of Massachusetts Lowell (Faculty start-up D50210000000022 from Y-JL).</p>
</sec>
<ack>
<p>The authors express gratitude to all the participants in this study.</p>
</ack>
<sec sec-type="COI-statement" id="sec30">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of int.</p>
</sec>
<sec sec-type="disclaimer" id="sec31">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<sec id="sec32" sec-type="supplementary-material">
<title>Supplementary material</title>
<p>The Supplementary Material for this article can be found online at: <ext-link xlink:href="https://www.frontiersin.org/articles/10.3389/fpsyg.2024.1403146/full#supplementary-material" ext-link-type="uri">https://www.frontiersin.org/articles/10.3389/fpsyg.2024.1403146/full#supplementary-material</ext-link>.</p>
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<supplementary-material xlink:href="Table_3.docx" id="SM3" mimetype="application/vnd.openxmlformats-officedocument.wordprocessingml.document" xmlns:xlink="http://www.w3.org/1999/xlink"/>
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