The genus Nicotiana originated in South America and subsequently spread to multiple continents, including Australia and the South Pacific (Goodspeed, 1947). Nicotiana benthamiana (N.benthamiana), a native Australian species, diverged from its ancestral lineages approximately 20 million years ago and successfully adapted to dramatic climatic changes that transformed large parts of the Australian continent into arid regions (Goodspeed, 1947). These evolutionary processes resulted in a species with distinct genetic and physiological characteristics (Figure 1). These features later proved advantageous for experimental research (Baulcombe, 2004).

N.benthamiana belongs to the Solanaceae and is a heterotetraploid species formed through ancient hybridization events, and has a genome with 19 chromosomes (2n=19). Comparative genomic analyses have revealed that its genome originated from hybridization between ancestral lineages related to Nicotiana glauca and Nicotiana sylvestris, with subsequent polyploidization contributing to genetic redundancy and functional diversification (Kelly et al., 2013; Schiavinato et al., 2020). Such genomic features are thought to enhance phenotypic plasticity and may partially explain the exceptional adaptability and experimental responsiveness of N.benthamiana (Wang et al., 2024).

Beyond its evolutionary background, N.benthamiana has become a premier model plant due to a unique combination of experimental advantages. It is easy to cultivate, generates abundant biomass, and exhibits well-characterized patterns of gene expression regulation and post-translational modification (Bally et al., 2018). Importantly, N.benthamiana is highly susceptible to a broad range of plant viruses and supports an efficient Agrobacterium-mediated transient expression system. These properties enable rapid functional analyses of gene regulation, protein-protein interactions, and subcellular localization.

The exceptional sensitivity of N.benthamiana to viral infection, coupled with its amenability to transient and stable genetic manipulation, has positioned it as a central model for dissecting plant-pathogen interactions. In this review, we summarize recent mechanistic studies investigating interactions between N.benthamiana and diverse pathogenic microorganisms, with a particular focus on immune signaling and pathogen virulence strategies. This synthesis provides a framework for future applications of N.benthamiana in plant disease resistance research and its development as a plant-based biotechnological platform.

Throughout evolutionary history, interactions between plants and pathogens have been sharped by continuous selective pressures, with plant-virus interactions representing a particularly dynamic antagonistic relationship. RNA silencing is an important immune mechanism for plant defense against aberrant expression of exogenous RNAs, and the initiation of this mechanism in plants is largely dependent on the RDRPs gene family (Baulcombe, 2004). In N.benthamiana, the initiation of RNA silencing is usually associated with the RDR1 and RDR6 genes, and NbRDR1 exhibits symptoms of susceptibility to viral infestation due to the insertion of a 72-bp fragment, e.g., high susceptibility to tobacco mosaic virus (TMV). The Arabidopsis RDR6 (AtRDR6) homolog NbRDR6, on the other hand, is very conserved and is involved in responding to viruses such as cucumber mosaic virus (CMV) and potato X virus (PVX). In addition, RDR6 is involved in short-range intercellular RNA silencing in N.benthamiana(Qin et al., 2012).

In research of N.benthamiana’s interactions with viruses, despite the fact that both the RDR1 and RDR6 genes play important roles in plant RNA silencing, there are significant differences in the way their genes are expressed. RDR1 is inducibly expressed and is only expressed in response to inducers such as salicylic acid (SA) or jasmonic acid (JA) (Pandey and Baldwin, 2007; Xu et al., 2003). Yang (Yang et al., 2004) demonstrated that N.benthamiana carries a naturally mutated, nonfunctional RDR1 allele (NbRDR1m). Although NbRDR1m transcription is induced by salicylic acid following TMV infection, a premature stop codon prevents the production of a full-length functional protein. As a result, NbRDR1m fails to restrict viral accumulation and does not effectively limit disease progression.The conclusion that NbRDR1 is not functional or has reduced activity is supported by the fact that transgenic plants inoculated with TMV showed improved lethality by transgenic transfer of Medicago truncatula RDR1 gene (MtRDR1) into N.benthamiana (Lee et al., 2016), and that TMV infestation or SA induction can elicit NtRDR1 activity in N.tabacum, and the accumulation of viral RNA was significantly higher than that of the wild type when the RDR1-deficient mutant N.tabacum was infested with TMV, but the viral proliferation was inhibited and the viral RNA content decreased in the SA-treated RDR1-deficient N.tabacum (Xie et al., 2001). These results suggest that SA possesses both an RDR1 dependent and an independent defense pathway.

RDR6 is constitutively expressed and is involved in RNA silencing independently of spatiotemporal specificity. NbRDR6 removes viruses from root tip growth point meristematic tissues and inhibits the systemic spread of PVX (Schwach et al., 2005). It has been noted that RDR1 and RDR6 are also not exactly two independent RNA silencing initiation pathways. The introduction of NtRDR1 into N.benthamiana by transgenic means was intended to compensate for its immunodeficiency. However, this resulted in the suppression of NbRDR6-dependent post-transcriptional gene silencing. The phenotype resembled that observed following NbRDR6 gene silencing.(Ying et al., 2010), which suggests that RDR1 has the function of suppressing RDR6-mediated antiviral RNA silencing. RDR1 is mainly involved in plant defense against viruses, whereas RDR6 has the same ability to defend against viruses and is also involved in the regulation of adversity response and growth and development (Qu et al., 2005; Peragine et al., 2004; Yang et al., 2011). Interestingly, according to Ying’s research, transfection of the active RDR1 gene into N.benthamiana surprisingly increased the susceptibility of tobacco viruses other than TMV, Turnip vein-clearing virus (TVCV), and Sunn hemp mosaic virus (SHMV), such as Plum pox potyvirus (PPV), CMV, PVX, and potato Y virus (PVY), which explains the inhibitory effect of RDR1 expression on the function of RDR6 (Ying et al., 2010). These observations suggest that the high viral sensitivity of N.benthamiana may have imposed strong selective pressure, favoring natural mutation of NbRDR1 to maintain broad-spectrum antiviral defense mediated by NbRDR6. Moreover, the transition of central Australia from humid to arid conditions during N.benthamiana evolution may have further selected for NbRDR1 inactivation, thereby enhancing NbRDR6-dependent stress and virus resistance and facilitating adaptation to climate change.

Dependence on RDR1 and RDR6 in plants can produce many dsRNAs, which subsequently undergo Dicer-Like (DCL) cleavage to form sRNAs (including siRNAs, miRNAs) induced with Argonaute (AGO) proteins to form the RNA-induced silencing complex (RISC), which can block expression of viral complementary RNAs by targeting the viral complementary RNAs to degrade or translationally repress them thereby blocking expression (Szittya and Burgyán, 2013; MaChado et al., 2017; Ma and Zhang, 2018; Yang and Li, 2018; Iwakawa and Tomari, 2022).

In A.thaliana, AGOs 1, 2, 4, 5, 7 and 10 are specific in virus defense (Carbonell and Carrington, 2015), and N.benthamiana may be similar. It has been shown that both NbAGO1 and NbAGO4 genes are required for systemic silencing in N.benthamiana by means of Virus-Induced Gene Sliencing (VIGS) (Jones and Dangl, 2006). Infection of N.benthamiana using tomato ringspot virus (ToRSV), followed by silencing of NbAGO1, prevents the recovery of necrotic symptoms in infected leaves with inhibition of viral translation, thus also demonstrating that the NbAGO1 protein is essential (Ghoshal and Sanfaçon, 2014). NbAGO2 has been frequently reported in studies, likely reflecting the central antiviral roles of AGO1 and AGO2 identified in A.thaliana. AGO1 acts as the primary defense by efficiently silencing exogenous RNA, whereas AGO2 serves as a secondary barrier that restricts viral accumulation. However, AGO1- and AGO2-mediated defenses are not fully simultaneous, as AGO2 activity is suppressed by miR403 when AGO1 is engaged (Harvey et al., 2011). AGO2-mediated antiviral defense is generally activated when AGO1-dependent primary defense is compromised, such as during infection by AGO1-suppressing viruses or artificial repression of AGO1. However, in N.benthamiana, repression of miR403, which negatively regulates NbAGO2, can activate NbAGO2 antiviral activity without disrupting AGO1 function. This challenges the prevailing view that AGO2 activation strictly depends on AGO1 inactivation.(Harvey et al., 2011; Diao et al., 2019). Scholthof utilized the VIGS technique using Tobacco rattle virus (TRV) as a vector to silence the expression of NbAGO2 (Scholthof et al., 2011). When this gene expression was silenced, no significant effects on normal growth and development were observed in N. benthamiana; however, the plants displayed increased susceptibility after inoculation with Tomato bushy stunt virus (TBSV) (Scholthof et al., 2011). Following this, considering that by means of VIGS a virus would need to be introduced in the host, which could have an impact on the true phenotype by disrupting many of the host’s functions, the dsRNA hairpin was used instead to VIGS of NbAGO2, which resulted in a phenotype consistent with the previous one (Odokonyero et al., 2015). These two studies collectively demonstrate that NbAGO2 plays a facilitative role in antiviral defense. In addition to the above reports, there is still a great deal of enigma surrounding NbAGOs in N.benthamiana, and it remains to be further confirmed whether the remaining AGO proteins in A.thaliana that are more homologous to those in N.benthamiana also have specific antiviral effects in N.benthamiana.

N.benthamiana has four differently acting DCL proteins that each produce four distinct populations of small RNAs that exercise functions in subsequent binding to different AGO proteins. DCL2 and DCL4 are commonly thought to be associated with antiviral defense (Parent et al., 2015; Qin et al., 2017). A study in N.benthamiana verified that there was no significant increase in viral content in plants when DCL2 and DCL4 were interfered with the expression of only one of the proteins by RNAi, but there was a significant increase in viral content when both were interfered with at the same time (Katsarou et al., 2019). This result confirms that DCL2 and DCL4 are associated with antiviral defense as well as the functional redundancy between the two. In a sresearch by Wang (Wang et al., 2024) Chromosome-level genome annotation indicates that N.benthamiana retains single copies of RDR6, DCL2, DCL3, and AGO2 following subgenome loss, and possesses fewer AGO1 and AGO4 homologs compared with Nicotiana tabacum. Such reductions in RNA silencing-associated gene copy number may partially underlie the increased viral susceptibility of N.benthamiana.

Geminiviruses are among the most serious viruses affecting plants globally, posing a serious threat to the safety of economic and food crops such as tobacco, tomato, and cassava in particular (Li et al., 2018; Chen et al., 2013; Allie et al., 2014). As the most abundant plant DNA virus, its interactions with plants involve both the same PTGS pathway as against RNA viruses and a variety of different mechanisms, and its complete infection cycle remains unelucidated (Kumar, 2019; Aguilar et al., 2020; Liu et al., 2021). The genomes of Geminiviruses may have either one or two single-stranded circular DNAs, and containing a single ssDNA type requires the presence of satellite DNAs, while containing two ssDNA types usually requires the presence of both DNA-A and DNA-B to trigger systemic infection, both of which are rolled-over replicating in the host (Aguilar et al., 2020; Briddon and Stanley, 2006). Among all plant viruses, DNA viruses are not as diverse as RNA viruses, and among the few plant DNA viruses, Geminiviruses are currently the most widely studied. Therefore, the section on N.benthamiana interactions with plant DNA viruses is reviewed using representative Geminiviruses as examples.

Plants share the same primary defense mechanism against DNA viruses and RNA viruses, both of which rely on RNA silencing to block post-transcriptional expression, so natural mutations in N.benthamiana RDR1 also affect interactions with DNA viruses (Akhtar et al., 2011; Prakash et al., 2020). However, RDR1 can be able to enhance the methylation of the viral genome against Geminiviruses thereby alleviating symptoms (Basu et al., 2018). At this time the properties of RDR1 as a defense protein help to reduce the abundance of viral transcripts in plants and increase the level of siRNAs (Basu et al., 2018). Loss of functional NbRDR1 equates to loss of this mechanism, and thus N.benthamiana is more defective in defense against DNA viruses compared to defense against RNA viruses.

Geminiviruses generally require the presence of both DNA-A and DNA-B in the host to cause systemic infection. It has been well documented that, influenced by innate susceptibility, the presence of only a portion of the virus’ DNA-A in N.benthamiana is sufficient to cause infection (Saunders et al., 2002; Galvao et al., 2003; Wang et al., 2014). In the presence of both DNA-A and DNA-B of Indian cassava mosaic virus from Singapore (ICMV-SG), the infected leaves of Jatropha curcas show yellowish-green mosaic, curling, deformities, overall stunted development of infected plants as well as fewer or even completely sterile fruits, but DNA-A expressing ICMV-SG in N.benthamiana resulted in the above mentioned conditions (Wang et al., 2014). This result demonstrates that N.benthamiana has the potential to explore the respective functions of the two parts of the DNA of Geminiviruses.

Viruses also have their unique means of interfering with plant defenses when plants defend themselves against viruses. The ability to regulate DNA methylation has been reported in Geminiviruses-plant interactions (Gui et al., 2022; Guo et al., 2022). On the one hand, plant RDR1 inhibits viral replication by methylation modification of virus DNA as described above, and on the other hand, the virus can regulate demethylation to disrupt methylation-mediated defenses (Gui et al., 2022). N.benthamiana defects cause a loss of function in the former, and thus the latter demethylation is more active, which may also be the reason for the N.benthamiana effect on biolytic virus replication (Gui et al., 2022). N.benthamiana is susceptible to Geminiviruses. Geminiviruses can encode a variety of small proteins, Tomato yellow leaf curl virus (TYLCV) encodes RNA silencing inhibitory protein V3 (Gong et al., 2021), and the AV2 protein of Tomato leaf curl Palampur virus (ToLCPalV) enhances viral virulence and accelerates N.benthamiana systemic infection. N.benthamiana system necrosis and accumulation of reactive oxygen species (ROS) (Roshan et al., 2018). The current study of viral small proteins using N.benthamiana as a model is shown in Table 1.

Viroids are a class of small ring-closed, single-stranded RNA molecules without a protein capsid envelope, whose genomes must interact directly with host components in order to replicate themselves and induce disease in the host (Flores et al., 2015). Compared to plant viruses we know little about the interactions between viroids agents and plants. Viroid species have been shown to trigger the RNA silencing pathway, but the role of the RNA silencing pathway in viroid infections is unclear (Li et al., 2021).

When a viroid infects a plant, the viroid nucleic acid is subjected to degradation by the DCL protein into a 21–24 nt viroid-derived smallRNA (vd-sRNA), which is then assembled into a RISC for specific cleavage of homologous host mRNAs (Ma and Zhang, 2018). Infection of N.benthamiana with Potato spindle tuber viroid (PSTVd), the process accompanying the production of vd-sRNA by the above mechanism can trigger a series of molecular events leading to the emergence of macroscopic symptoms of viroid infections, which in N.benthamiana are manifested as leaf curling and developmental retardation (Navarro et al., 2021).

Plant-induced RNA silencing responses against viroids require the activities of both RDR1 and RDR6. To compensate for the loss of endogenous RDR1 function in N.benthamiana, NtRDR1 from N.tabacum was overexpressed. Upon inoculation with PSTVd, plants expressing NtRDR1 exhibited a marked reduction in viroid RNA accumulation (Li et al., 2021). This result demonstrates that the resistance of N.benthamiana to viroids is affected by the absence of NbRDR1 function, and that NbRDR6 plays an important role in the accumulation of viroid RNA and infection symptoms in N.benthamiana (Di Serio et al., 2010). When the NbRDR6 gene is repressed, symptoms do not appear at the beginning of infection despite high levels of viroid RNA accumulation, demonstrating that viroid-induced symptoms in N.benthamiana are dependent on the activity of NbRDR6 (Gómez et al., 2008), and that repression of NbRDR6 facilitates the accumulation of viroid RNA (Adkar-Purushothama and Perreault, 2019).

Unlike the case of plants against Euvirus, DCL4 was mentioned as an antiviral-associated DCL protein in N.benthamiana against RNA virus infestation, so when DCL4 expression is inhibited it results in a large increase in viral content, but paradoxically DCL4 inhibition leads to a decrease in viroid content (Dadami et al., 2013; Katsarou et al., 2016). The above findings were confirmed in N.benthamiana, where the interaction between viroids and RNA silencing defied the prevailing antiviral mechanism, reaffirming the idea that viroids need to rely on RNA silencing in plant in order to accomplish substantial replication.

Viroid pathogenicity largely resembles endogenous microRNA-mediated regulation of plant growth and development, a process essential for viroid replication while also engaging host defense responses. Owing to its high susceptibility, N.benthamiana provides an effective system for dissecting viroid infection mechanisms and host resistance, although many aspects of these interactions remain to be elucidated.

Bacterial-plant interactions are highly complex, N.benthamiana tissues contain abundant extracellular hydrolytic and antimicrobial enzymes that limit bacterial colonization and can compromise bacterial integrity, thereby reducing virulence (Sueldo et al., 2023). These hydrolytic enzymes accumulate during the invasion process, so that the pathogenic bacteria need either a strong enough cell wall or secondary metabolites that inhibit hydrolytic enzyme activity in order to further infest the tissue and cause disease (van Loon et al., 2006).

Upon successful pathogen invasion, plants employ two major immune strategies: Pathogen-AssociatedMolecular Pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI). PTI relies on PRR-mediated recognition of PAMPs at the cell surface, whereas ETI involves direct or indirect recognition of pathogen avirulence (Avr) proteins by intracellular NBS-LRR resistance proteins. Both PTI and ETI activate the mitogen-activated protein kinases (MAPK) signaling cascades that contribute to enhanced disease resistance and localized hypersensitive responses (HR) (Jones and Dangl, 2006; Schwessinger and Ronald, 2012; Qian and Liu., 2014).

In the early stages of infestation without releasing effectors, Pattern Recognition Receptors (PRRs) on plant cell membranes specifically recognize pathogenic bacterial PAMPs to induce a defense response. Most of the plant PTI studies have centered around flagellar proteins (Felix et al., 1999; Chinchilla et al., 2006). Recognition of flagellar proteins of Pseudomonas syringae by N.benthamiana is dependent on a homolog of the A.thaliana AtFLS2, NbFLS2 protein, which specifically recognizes a conserved flg22 polypeptide on the bacterial flagellum to induce PTI. NbFLS2 silenced N.benthamiana will not respond to flagellin induction and the growth of various pathogens will be dramatically enhanced, confirming that NbFLS2 plays an important role in flagellar perception (Hann and Rathjen, 2007). Elongation factor thermo unstable (EF-Tu) is a specific PAMP, and when a pathogen comes into contact with plant cells, the EF-Tu receptor (EFR) specifically recognizes the pathogen to release EF-Tu N-terminal elf18, producing a pathogen defense response that is highly similar to the flagellin-induced defense response (Kunze et al., 2004). EF-Tu is unique in that its receptor, EFR, is only found in A.thaliana and other Brassicaceae (Kunze et al., 2004). Agrobacterium is currently the most widely used plant genetic transformation system (Horsch et al., 1985), an EF-Tu-containing pathogen, when infected with A.thaliana, induces a ROS burst, which accelerates ethylene synthesis and other drastic defense responses that limit transformation of A.thaliana by Agrobacterium. In contrast, N.benthamiana, which is not a member of the Brassicaceae, is not responsive to EF-Tu and is not limited by this immune response, which confers the ability to efficiently transform N.benthamiana T-DNA and transient transfection (Zipfel et al., 2006).

The release and translocation of effectors of pathogenic bacteria into plant cells followed by specific recognition with the corresponding nucleotide-binding leucine-rich repeat (NLR) immune receptors leads to the activation of NLR proteins to induce ETI (Cui et al., 2015). In the ETI signaling pathway, NLR proteins require the cooperation of other molecules to be activated properly, and it has been confirmed by VIGS in N.benthamiana that the trio of NbSGT1, NbEDS1, and NbRAR1 functions to help NLR proteins form complexes with effectors (Peart et al., 2002; Liu et al., 2002b). Using N.benthamiana as a model to explore effectors XopQ and HopQ1 produced by Xanthomonas and Pseudomonas, respectively, Schultink found that a TIR-NLRs (TLRs) Recognition of XopQ 1 (Roq1) was able to specifically recognize XopQ and HopQ1 and confirmed that Roq1 is an indispensable component of Nicotiana genus resistance to Xanthomonas and Pseudomonas, and reported that the perception of XopQ and HopQ1 is dependent on EDS1 (Schultink et al., 2017). On this basis Qi further complemented the interaction of N requirement gene 1 (NRG1) with EDS1 downstream of Roq1 and EDS1 to mediate ETI triggered by pathogenic XopQ and HopQ1, which ultimately leads to HR (Qi et al., 2018), a simple model of this signaling pathway is shown in Figure 2.

Potato late blight disease is a disease caused by Phytophthora infestans (P. infestans) infection, which is characterized by mildew on stems, leaves, and tubers that begins as yellowish-brown spots and rapidly expands to form dense white mold in humid climates, resulting in plant rot (Fry, 2008). The range of species infected by P.infestans is very narrow, generally occurring only in the Solanaceae, so N.benthamiana, which is related to cash crops such as tomato and potato, has become the most widely used model plant for the study of P.infestans-plant interactions (Kamoun et al., 1993; Kamoun et al., 1998).

INF1 is a class of elicitin proteins secreted by P.infestans that act as PAMPs in interactions with N.benthamiana (Kamoun et al., 1998). INF1 can localize to the extracellular compartment of N.benthamiana tissues, interacts with the receptor complex containing NbLRK1 on the surface of the plasma membrane and recognizes INF1, triggering downstream H₂O₂ production and HR signaling (Kanzaki et al., 2008). In vitro experiments have demonstrated that INF1 interacts with the subdomain VIb recognition of NbLRK1 intracellular kinase activity, and under natural conditions it is dependent on a number of proteins, including Hsp90, Hsp70, and SGT1, to successfully activate downstream cascade reactions (Kanzaki et al., 2008; Shibata et al., 2011). However, there are still many mysteries to be solved in the study of INF1. No research has been able to show how INF1 enters N.benthamiana cells and acts on NbLRK1 intracellular structures, and the composition of the NbLRK1 receptor complex remains unknown, although INF1-interacting membrane proteins have been validated in vitro (Kanzaki et al., 2008). On the other hand, N.benthamiana exportin 1 (NbXpo1) is required for INF1-induced defense responses, and in mammals and fungi exportin1 is thought to be a regulator of protein and RNA export through the nuclear pore, and NbXpo1-silenced N.benthamiana exhibits a slight growth defects, as well as inhibition of INF1-induced cell death, significantly reducing resistance to P.infestans (Mizuno et al., 2019). The heteropolyploid nature of N.benthamiana may underlie gene copy expansion, as A.thaliana contains two Xpo1 genes (AtXpo1a and AtXpo1b), while N.benthamiana possesses four Xpo1 homologs. A similar pattern is observed for BAK1 and its homolog NbSerk3.(Blanvillain et al., 2008; Goodin et al., 2008). VIGS-mediated silencing of NbSerk3A and NbSerk3B in N.benthamiana significantly attenuated the hypersensitive response induced by purified INF1. These results indicate that NbSerk3A and NbSerk3B likely contribute to INF1-triggered cell death during Phytophthora infestans infection(Chaparro-Garcia et al., 2011).

Fungal pathogens pose a serious threat to terrestrial plants, contributing to substantial yield losses of approximately 10-23% annually and further post-harvest losses of 10-20%. The high susceptibility and experimental accessibility of N.benthamiana make it a useful system for studying fungal pathogenesis and plant defense mechanisms (Stukenbrock and Gurr, 2023). Nowadays, fungi have become a great threat to food security, and the large-scale cultivation of monocultures in the field provides sufficient nutrient conditions and suitable breeding sites for fungi, and the use of antifungal agents under such conditions leads to the screening of resistant strains in a short period of time and their rapid multiplication (Fisher et al., 2018). Therefore, in this context, where the rate of fungal resistance formation is much faster than the rate of development of new antifungal agents, the study of plant-fungal interactions stands out.

N.benthamiana is an excellent model plant for studying the relationship between plants and fungal pathogens (Goodin et al., 2008). Fungal infection of plants begins with contact at the cell wall level, and the chitin-containing fungal cell wall is mechanically stronger than the tough and elastic bacterial cell wall. Plants do not contain chitin components, so chitin in fungal cell walls is considered a non-self component to activate plant immune responses. In the last decade of research, chitin is the most typical PAMP for fungi to cause PTI in plants (Kombrink et al., 2011). During fungal infection of Arabidopsis, AtCERK1 and AtLYKs, members of the LysM-RLKs family, recognize chitin and activate downstream immune response pathways (Shinya et al., 2015). NbCERK1 has been shown to be a chitin receptor and is involved in positively mediating the chitin signaling pathway (Gimenez-Ibanez et al., 2009), the defense mechanism of N.benthamiana is similar to that of Arabidopsis, which is recruited by AtLYK5 to form a receptor complex with AtCERK1 to bind chitin, whereas N.benthamiana binds chitin through the triple NbERK1/NbCERK1/NbLYK4 receptor complex, while NbERK1 with kinase activity dissociates from the complex and positively regulates the immune response in response to chitin induction, enhancing plant resistance (Pi et al., 2023).

MAPK cascade reaction is a hallmark of defense signaling in plants such as Arabidopsis, tobacco, tomato, and N.benthamiana, and MAPK activity determines the effective level of basal plant resistance (Segonzac et al., 2011; Tanaka et al., 2009). Wound-inducible protein kinase (WIPK) and salicylic acid-inducible protein kinase, known as pathogen- responsive MAPKs, together comprise the MEK2-SIPK/WIPK cascade in N.benthamiana (Pedley and Martin, 2005). MEK2 is the common upstream kinase of SIPK and WIPK (Yang et al., 2001), and its active form, MEK2, has been shown to be the most effective kinase in N.benthamiana, and its active form, MEK2DD, induces phosphorylation of SIPK and WIPK, which in turn activates the downstream target WRKY transcription factor and elicits HR responses (Adachi et al., 2016). It has been demonstrated that phosphorylation of Positive Transcription Factor WRKY8 by SIPK and WIPK enhances DNA binding and transcriptional activity and increases expression of downstream defense genes in N.benthamiana. WRKY8 is also phosphorylated by NTF4, which is homologous to SIPK, and by NTF6 in the NPK1-MEK1-NTF6 cascade (Ishihama et al., 2011), as shown in Figure 3a. In N.benthamiana leaves, the MEK2-SIPK/NTF4 and MEK1-NTF6 cascades both regulate RBOHB, contributing to ROS bursts and restriction of lesion expansion; however, only MEK2-SIPK/NTF4 is required for effective ROS production and lesion containment. Furthermore, SIPK/NTF4 promotes nitric oxide (NO) accumulation by regulating Nitric Oxide-Associated 1 (NOA1), thereby enhancing basal resistance in N.benthamiana Figure 3b (Asai et al., 2008; Asai and Yoshioka, 2009).

Despite its widespread use and unique experimental advantages, N.benthamiana is not a universal model for all aspects of plant-pathogen interactions. A critical evaluation of its limitations is essential for the appropriate interpretation of experimental results and for avoiding overgeneralization.

N.benthamiana has been widely adopted as a model for plant-pathogen interaction studies, and insights gained from this system are broadly applicable to other Solanaceous species, including food crops such as tobacco, tomato, chili pepper, goji berry, and potato, as well as ornamental plants like Datura stramonium. Elucidating these interactions facilitates the improvement of resistance to biotic stresses while supporting plant growth and development.

N.benthamiana has emerged as an indispensable model system for dissecting plant-pathogen interactions, owing to its unique evolutionary background, genetic features, and exceptional experimental tractability. Its heteropolyploid genome, high susceptibility to diverse pathogens, and amenability to rapid genetic manipulation have enabled mechanistic insights into antiviral RNA silencing, immune signaling networks, and pathogen virulence strategies that are difficult to achieve in many other plant systems.

Studies in N.benthamiana have been particularly instrumental in elucidating RNA silencing-based antiviral defense, including the coordinated functions of RDRs, DCLs, and AGOs, as well as their complex regulatory relationships. Beyond viral systems, N.benthamiana has proven to be a powerful platform for investigating plant interactions with DNA viruses, viroids, bacteria, fungi, and oomycetes, revealing conserved and divergent features of PTI, ETI, MAPK signaling, and effector recognition across plant lineages (Figure 4). These findings highlight the value of N.benthamianaa as a unifying experimental framework for comparative analyses of plant immunity.

At the same time, the limitations of N.benthamiana as a model system must be carefully considered. Genetic peculiarities affecting RNA silencing, reliance on transient expression systems, and incomplete representation of complex immune dynamics underscore the need for cautious interpretation and validation in additional plant species. Rather than diminishing its utility, these caveats define the appropriate scope of N.benthamiana as a discovery-driven platform.

Looking forward, the integration of N.benthamiana-based approaches with emerging technologies will further enhance its capacity to uncover dynamic and context-dependent immune mechanisms. These technologies include single-cell and spatial omics, advanced imaging, and synthetic biology. Moreover, coupling discoveries made in N.benthamiana with translational studies in crop plants will be essential for converting fundamental insights into practical strategies for improving disease resistance and sustainable agriculture.

In summary, N.benthamiana will continue to serve as a cornerstone model for plant-pathogen interaction research, bridging fundamental mechanistic studies and applied plant science, and remaining central to future advances in plant immunity and biotechnology.