AUTHOR=De Meo Elisa , Baldisseri Anna , Loreti Elena , Perata Pierdomenico 

TITLE=RNA interference shapes stress responses in Arabidopsis thaliana

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1699198

DOI=10.3389/fpls.2025.1699198

ISSN=1664-462X

ABSTRACT=RNA interference (RNAi) is a crucial regulatory mechanism in plants, enabling dynamic responses to environmental stresses. Small RNAs (sRNAs), including miRNAs and siRNAs, guide stress-responsive gene silencing and can act beyond the cell of origin through systemic movement and uptake, enhancing plant adaptability. Central to these pathways are ARGONAUTE (AGO) proteins, whose stress-regulated expression and diverse roles at the molecular level contribute to finely tuned responses. This review integrates current progress in sRNAs systemic signaling, stress-responsive RNAi mechanisms, and AGO protein diversity into a coherent framework for understanding RNAi-driven stress adaptation. Emerging approaches such as high-resolution sRNA sequencing and single-cell transcriptomics are now enabling a deeper understanding of RNAi regulation with improved spatial and temporal resolution.