Front. Plant Sci. Frontiers in Plant Science Front. Plant Sci. 1664-462X Frontiers Media S.A. 10.3389/fpls.2025.1659418 Plant Science Original Research Sustainable remediation of heavy metal contaminated soil through phytostabilization with the in-situ immobilization by mercapto-based palygorskite Kuang Hongjie 1 2 Wu Bin 1 Peng Fengge 1 Yu Yahui 1 Ma Xiaohui 1 Shangguan Yuxian 2 * 1 State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu, China 2 Institute of Agricultural Resources and Environment, Sichuan Academy of Agricultural Sciences, Chengdu, China

Edited by: Alla Yemets, National Academy of Sciences of Ukraine (NAN Ukraine), Ukraine

Reviewed by: Xiaokai Zhang, Jiangnan University, China

Xiaojun Zheng, Jiangsu University of Science and Technology, China

*Correspondence: Yuxian Shangguan, 396478825@qq.com

24 10 2025 2025 16 1659418 04 07 2025 06 10 2025 Copyright © 2025 Kuang, Wu, Peng, Yu, Ma and Shangguan. 2025 Kuang, Wu, Peng, Yu, Ma and Shangguan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Phytostabilization has been widely applied to remediate mining soils contaminated with heavy metals, but the high soil toxicity often restricts plant growth and remediation efficiency. In this experiment, we investigated the effect of mercapto-based palygorskite (MPAL, applied at 2% and 4% w/w) on cadmium (Cd) and lead (Pb) phytostabilization by Chrysopogon zizanioides in soils contaminated with Cd and Pb. The results showed that soil pH did not vary under the application of MPAL but decreased during the cultivation of C. zizanioides. Compared with planting C. zizanioides alone, the application of MPAL significantly promoted the growth of C. zizanioides, enhanced its antioxidant enzyme activities, and decreased Cd and Pb concentrations in roots and shoots. Compared with CK, the addition of 4% MPAL, cultivated with C. zizanioides, reduced DTPA-extracted Cd and Pb in soils by 89.22% and 51.18%, respectively, at the highest level (p < 0.05). Moreover, urease, cellulase, and sucrase activities in soils treated with MPAL and cultivated with C. zizanioides were enhanced, with maximum increases of 37.70%, 110.22%, and 42.99%, respectively (p < 0.05). The interaction of MPAL and C. zizanioides increased bacterial richness and diversity but did not alter the bacterial community. This study demonstrated that the use of MPAL in combination with C. zizanioides could serve as a potential strategy for Cd and Pb immobilization and improvement of soil microecological properties.

 Graphical Abstract

Illustration depicting the role of phytostabilization in reducing cadmium (Cd) and lead (Pb) in soil using C. zizanioides. Arrows indicate the uptake and immobilization process within plant roots. Text highlights MAPL's positive interactions with soil micro-ecology. Bar graphs show urease activity and cadmium levels for different treatments, with labels for significance. A community bar plot illustrates microbial diversity.

mercapto-based palygorskite Cd and Pb contamination heavy metal immobilization Chrysopogon zizanioides microbial diversity section-in-acceptance Plant Abiotic Stress Introduction

Soil heavy metal pollution has become a worldwide environmental concern (Alam et al., 2024). Heavy metals in soils pose significant ecological risks, resulting in soil fertility decline and plant damage and ultimately transferring into the human body through the food chain (Azeez et al., 2019; Gravand et al., 2020; El-Sharkawy et al., 2023; Kama et al., 2024). Mining and smelting activities are among the major sources of heavy metal contamination, where metals frequently accumulate in soils at concentrations far exceeding regulatory thresholds (Cuypers et al., 2010; Antoniadis et al., 2019). Among these metals, cadmium (Cd) and lead (Pb) are particularly problematic due to their high toxicity and mobility, which have caused severe harm to both human health and the environment. While numerous studies have investigated remediation approaches for single-metal contamination (e.g., Cd or Pb), most mine-affected soils are simultaneously contaminated with Cd and Pb. Remediation strategies effective for individual metals often prove inadequate in multi-metal scenarios (Liu et al., 2000; Huang et al., 2009). More importantly, the immobilization mechanisms of Cd and Pb differ (Yang et al., 2022b), making it especially challenging to simultaneously stabilize both metals. Therefore, there is an urgent need to develop cost-effective and eco-friendly remediation strategies suitable for Cd and Pb co-contamination.

Phytostabilization has been recognized as a gentle remediation option that employs heavy metal-tolerant plants, often assisted by soil amendments, to reduce the mobility and bioavailability of toxic metals in soils. The main mechanisms include metal adsorption and precipitation in the rhizosphere, complexation with root exudates and cell walls, and sequestration into root vacuoles, which together limit their translocation to shoots. Through these processes, phytostabilization minimizes heavy metal entry into the food chain and groundwater, while improving soil quality and stability (Madrid et al., 2008; Shackira and Puthur, 2019; Zhan et al., 2020). It has been widely considered in soil remediation due to its economic, eco-friendly, and sustainable nature (Baker et al., 1994; Ali et al., 2013; Zheng et al., 2024). However, conventional heavy metal-tolerant plants are limited by biomass and growth conditions, resulting in low remediation efficiency (Shen et al., 2022). Chrysopogon zizanioides is a widespread perennial herb with a well-developed root system, high germination rates and substantial biomass production (George et al., 2017; Pandey and Praveen, 2020). In addition, C. zizanioides is highly adaptable to saline, calcareous, and nutrient-deficient soils, and can even tolerate high concentrations of heavy metals (Edelstein et al., 2009; Masinire et al., 2021). Recent studies have demonstrated that C. zizanioides has significant potential for Cd and Pb phytostabilization in soils (Ai et al., 2023). Pidatala et al. (2018) observed that the Pb uptake by C. zizanioides root reached 3.86 × 103, 9.83 × 103, 1.85 × 104 mg·kg-¹ dry weight in Pb solutions at concentrations of 400 mg·L-¹, 800 mg·L-¹ and 1200 mg·L-¹, respectively. Previously, we also found that the concentration of Cd in C. zizanioides root was 18.4-fold higher than that found in the soil. In addition, it was reported that the ability of C. zizanioides to absorb Cd and Pb was greater under combined contamination conditions than under single-metal contamination (Ai et al., 2023).

Nevertheless, the biotoxicity of high concentrations of Cd and Pb in mining soils can hinder plant growth and reduce the phytostabilization efficiency of C. zizanioides in contaminated soils, and it has limited the application on mine rehabilitation. In-situ immobilization by applying active amendments to reduce the bioavailability of heavy metals has been proven to be an effective strategy to promote plant growth (Zheng et al., 2021; Lu et al., 2025). Common amendments, including biochar, liming, and phosphate mineral, reduce the bioavailability of Cd and Pb primarily by increasing soil pH (Song et al., 2022), but these amendments have little efficiency in alkaline soils (Yong et al., 2022). In this context, mercapto-modified palygorskite (MPAL) exhibits unique advantages in alkaline soils, where its surface mercapto groups can strongly bind Cd and Pb, effectively reducing metal bioavailability while maintaining soil chemical stability (Yong et al., 2024). Yang et al. (2022a) found that MPAL could significantly reduce the bioavailability of Cd in alkaline soils. The mercapto groups in the surface of MPAL have a strong affinity for Cd (Ksp of CdS = 8 × 10-27) and Pb (Ksp of PbS = 3.4 × 10-28). Our previous studies have demonstrated that MPAL application enhanced the microecology of Cd contaminated soils by improving the diversified sulfur metabolism biomarkers and decreasing the Cd resistance genes (Li et al., 2022). In addition to reducing Cd and Pb bioavailability, MPAL application could increase the abundance of plant growth-promoting bacteria thereby promoting plant growth. The evidence indicated that MPAL-assisted phytostabilization could provide an innovative approach to achieve Cd and Pb immobilization within a short time, especially in alkaline soils.

Herein, we hypothesize that MPAL application could provide a novel approach that simultaneously promotes C. zizanioides growth, immobilizes Cd and Pb, and improves rhizospheric microecology, thereby facilitating Cd and Pb phytostabilization in contaminated alkaline soils. The primary objectives of this study were to evaluate the effects of varying MPAL concentrations on Cd and Pb phytostabilization by analyzing plant biomass, antioxidant enzyme activities, Cd and Pb contents in plants, and soil Cd and Pb bioavailability and species distribution. Meanwhile, the effects of MPAL and C. zizanioides on rhizospheric microecology, including soil pH, enzyme activities, and microbial diversity and composition, were also examined.

 Materials and methods Soil and materials

The soil used in the experiment was collected from farmland in Chengdu, Sichuan Province, China. The collected soil samples were air-dried then sieved through a 2 mm mesh. After that, CdCl2 and Pb(NO3)2 solution were added, and the final Cd and Pb concentrations were 3 mg·kg-¹ and 700 mg·kg-¹, respectively, which represent typical levels of Cd and Pb contamination in mining soils and have been reported in previous studies (Zhou et al., 2024; Setu and Strezov, 2025). The spiked soils were incubated for a period to allow the metals to equilibrate with the soil matrix. This “aging” process ensured that the bioavailability and environmental behavior of Cd and Pb were similar to those in naturally contaminated soils, providing a reliable basis for subsequent remediation experiments. Pre-analytical assessments were conducted to confirm that the target concentrations of Cd and Pb had been successfully achieved. The resulting artificially contaminated soil was designated as the CK treatment (containing added Cd and Pb, without any amendments or plants) and served as the baseline control for all subsequent pot experiments.

The natural PAL was purchased from the Lingshou Qiangdong Mineral Processing factory (Shijiazhuang, China). MPAL was synthesized according to the method described by Fu et al. (2021). The successful mercapto functionalization of palygorskite (MPAL) was confirmed in our previous study (Li et al., 2022) by EDS analysis, which revealed distinct sulfur peaks with uniform distribution on the MPAL surface. FTIR and XRD analyses further verified the incorporation of mercapto groups while preserving the mineral framework. The Academy of agricultural science of Jiangsu province offered C. zizanioides seeds. All reagents used in this study were of analytical grade.

 Pot experiment design

The pot experiment was conducted in the Chengdu University of Technology greenhouse. Ten different treatments were designed as follows: CK (Contaminated soil without treatment), 2% PAL (Contaminated soil treated with 2% PAL), 4% PAL (Contaminated soil treated with 4% PAL), 2% MPAL (Contaminated soil treated with 2% MPAL), 4% MPAL (Contaminated soil treated with 4% MPAL), P (Contaminated soil treated with plant), 2% PAL + P (Contaminated soil treated with 2% PAL and plant), 4% PAL + P(Contaminated soil treated with 4% PAL and plant), 2% MPAL + P (Contaminated soil treated with 2% MPAL and plant), 4% MPAL + P (Contaminated soil treated with 4% MPAL and plant). Each treatment was performed with three biological replicates, that is, three independent pots per treatment. Each pot was filled with 3 kg of aged soil, and the soil was thoroughly mixed with PAL and MPAL in different mass percentages according to treatment conditions. Seeds of C. zizanioides were germinated in a constant temperature incubator at 25°C, and 20 of healthy and uniform sprouts were transplanted into each pot. C. zizanioides were grown in a greenhouse at a temperature range of 25–28°C, watered daily with deionized (DI) water to 60% of the field water capacity, and harvested after 60 days.

 Plant analysis Plant growth response

The C. zizanioides were carefully harvested and rinsed with DI water. The fresh and dry biomass were weighed. Then, 0.1 g of leaves were ground in 2 mL of phosphate buffer solution (PBS, pH=7.8) with liquid nitrogen, and then the homogenate was centrifuged at 4°C (10000 rpm, 10 min) to obtain enzyme solution. After that, peroxidase (POD) activity was determined using guaiacol as a substrate (Liao, 2015). Superoxide dismutase (SOD) activity was determined using nitroblue tetrazolium (NBT) (Durak et al., 1993). Catalase (CAT) activity was determined by monitoring the decrease in absorbance at 240 nm caused by H2O2 consumption (Kolahi et al., 2020). Soluble protein content was measured using the Coomassie brilliant blue method (Bradford, 1976). The hydrogen peroxide content was measured following the method of Uchida (Uchida et al., 2002). The production rate of superoxide anion radical was measured using the hydroxylamine hydrochloride oxidation method.

 Heavy metal analysis

0.1 g of dried plant powder was weighed and digested in a mixture of HNO3, HF, and HClO4 (5:5:3, v/v/v) at 180°C using an electric hot plate. Then, the digested solution was diluted to a final volume of 10 mL with 1% HNO3 (Wu et al., 2018). The mixture was centrifuged at 3000 rpm for 20 min, and the Cd and Pb concentrations in supernatants were measured by atomic absorption spectroscopy (AAS). Method blanks, duplicate samples, and certified reference materials (CRMs) were included to ensure reliability. Recoveries of CRMs ranged from 90% to 105%, and relative standard deviations (RSDs) for replicates were generally below 15%.

 Soil analysis Soil pH and heavy metals

Soil pH was measured using a pH meter (METTLER-S220). The available heavy metal was extracted by using diethylenetriamine pentaacetic acid (DTPA). Metal fractions were extracted using the BCR sequential extraction procedure to evaluate metal bioavailability and transformation (Wu et al., 2018). Briefly: 1) 1 g of air-dried soil was mixed with 40 mL of 0.1 M CH3COOH and shaken at 150 rpm at 25 °C for 16 hours. After centrifugation, the supernatant was collected as the HOAc-extractable fraction, and the remaining soil was retained for the next step. 2) The retained soil was mixed with 40 mL of 0.5 M NH4OH·HCl (pH=2.0) and shaken at 150 rpm, 25°C for 16 h. After centrifugation, the supernatant was collected as the reducible fraction, and the residue was retained for the next step. 3) The residue was heated with 10 mL of 30% H2O2 (pH 2–3, 85°C) in a water bath until the solution volume was reduced to less than 1 mL. Then, 50 mL of 1 M NH4AC (pH=2) was added and shaken at 150 rpm, 25°C for 16 hours. After centrifugation, the supernatant was collected as the oxidizable fraction, and the residue was retained for the final step. 4) Finally, the remaining residue was digested in the same way as plant samples to obtain the residual fraction.

 Soil enzyme activity analysis

In this study, the activities of urease, catalase, sucrase and cellulase were determined. The activity of urease was determined by sodium phenol-sodium hypochlorite colorimetric method, the activity of catalase was measured by potassium permanganate titration, the activities of sucrase and cellulase were determined by the chromogenic reaction of 3, 5-dinitrosalicylic acid at 540 nm (Jiang et al., 2022).

 Microbial diversity analysis

The DNA from the soil sample was extracted with soil DNA extraction kit (Omega, Norcross, GA, U.S.), PCR amplification of 16S rRNA gene was performed with using universal primer pairs (343F: 5′-TACGGRAGGCAGCAG-3’; 798R: 5′-AGGGTATCTAATCCT-3′). QIIME2 was employed to analyze microbial sequencing data. The data of microbial community composition and alpha diversity were processed and analyzed through in the MajorBio cloud platform and then visualized by Origin 2021 software. Functional genes associated with sulfur metabolism was predicted from 16S rRNA gene data using the PICRUSt2 program, with functional annotations derived from the KEGG databases ( Supplementary Figure S1 ).

 Data analysis

In the experiment, each treatment was established with three biological replicates. The bioaccumulation factor (BCF) and translocation factor (TF) were calculated based on the methods of Yong et al. (2022) using Equations 1–3.

BCF = h e a v y   m e t a l  concentration  i n  plant h e a v y   m e t a l  concentration  i n   s o i l TF 1 = h e a v y   m e t a l   c o n c e n t r a t i o n   i n   s t e m h e a v y   m e t a l   c o n c e n t r a t i o n   i n   r o o t TF 2 = h e a v y   m e t a l   c o n c e n t r a t i o n   i n   l e a f h e a v y   m e t a l   c o n c e n t r a t i o n   i n   s t e m

The mean and standard deviation were calculated using IBM SPSS Statistics 25, statistical significance was assessed using one-way ANOVA and Duncan’s multiple range test, with a significance level of 5%.

 Results and discussion Effect on soil pH

Soil pH is a crucial factor affecting the bio-availability of heavy metals. Most amendments, such as lime, biochar, and manure have been shown to enhance soil pH to reduce the bioavailable heavy metals (Ruttens et al., 2010; Wang et al., 2014). However, the significant variation in pH can markedly affect the soil micro-ecology, including soil enzyme activities, microbial communities and functions (Jiang et al., 2021; Naz et al., 2022). Figure 1 illustrated that the application of PAL and MPAL has no significant effect on soil pH. This suggested that MPAL met the criteria for being environmentally friendly without changing soil pH. However, the cultivation of C. zizanioides resulted in a decrease in soil pH by 0.10 to 0.27. In general, plants can secrete low molecular weight acid (LMWA) to mobilize insoluble minerals to acquire essential nutrients, resulting in soil acidification (Marschner et al., 1987). A reduction in soil pH can increase the bioavailability of heavy metals, thus promoting the accumulation of heavy metals by plants (Zeng et al., 2011).

Soil pH under different treatments.

Line graph depicting soil pH levels under different treatments: CK, 2% PAL, 4% PAL, 2% MPAL, and 4% MPAL. Triangles represent unplanted vetiver, while circles represent planted vetiver. Soil pH ranges from 8.0 to 8.6, with higher pH in unplanted vetiver across treatments. Effect on soil Cd and Pb availability and geochemical fractions

As shown in Figure 2A , in the absence of C. zizanioides planting, the content of DTPA-extracted Cd in CK was 1.10 mg·kg-¹, which decreased by 5.73% - 10.96% and 73.54% - 86.93% in the PAL and MPAL treatments, respectively. Following the planting of C. zizanioides, the DTPA-extracted Cd further decreased by 4.63% - 17.48%. Most notably, the DTPA-extracted Cd showed the greatest decrease in the treatment of 4% MPAL with C. zizanioides planting, which was 89.22% lower than CK. As shown in Figure 2B , the content of DTPA-extracted Pb in CK was 293.26 mg·kg-¹, and the application of PAL and MPAL resulted in a significant reduction in the DTPA-extracted Pb content by 8.46% - 9.01% and 15.45% - 34.17%, respectively. The planting of C. zizanioides further decreased DTPA-extracted Pb by 13.31% - 38.18%.

DTPA-extractable (bioavailable) Cd (a) and Pb (b) concentrations in soils under different treatments. Mean with different letters indicated significant difference from each other according to one-way ANOVA and Duncan’s test (p < 0.05).

Bar charts comparing DTPA-extractable cadmium (Cd) and lead (Pb) concentrations under different treatments. Chart (a) shows Cd concentrations, with bars for unplanted and planted vetiver in pink and purple. Chart (b) shows Pb concentrations, with bars for unplanted and planted vetiver in light and dark blue. Treatments include CK, 2%PAL, 4%PAL, 2%MPAL, and 4%MPAL, with noticeable reductions in metal concentrations, particularly under 4%MPAL treatment. Error bars and statistical significance lettering are indicated above bars.

The various geochemical fractions of Cd and Pb were measured, and the results are presented in Figure 3 . The application of MPAL and C. zizanioides decreased the proportion of HOAc-extractable Cd and increased the proportion of reducible Cd. Under the treatment of 4% MPAL and C. zizanioides, the HOAc-extractable Cd content decreased significantly by 53.71%, while the reducible Cd content increased markedly by 111.55%. Similarly, MPAL-assisted C. zizanioides incubation decreased the proportion of HOAc-extractable Pb and increased the proportions of reducible Pb and oxidizable Pb. Under the treatment of 4% MPAL and C. zizanioides, the proportion of HOAc-extractable Pb decreased significantly by 37.58%, while the proportion of oxidizable Pb increased markedly by 207.43%. These results showed that the application of MPAL assisted C. zizanioides can effectively immobilize Cd and Pb in the co-contaminated soil.

Speciation analysis of Cd (a) and Pb (b) under different treatments.

Stacked bar graphs show the fractional percentages of cadmium (Cd) and lead (Pb) in different treatments. Graph (a) illustrates the distribution of Cd, while graph (b) presents Pb. Each bar divides into segments representing HOAc extractable, reducible, oxidizable, and residual forms. Treatments vary from CK to 4% MPAL+P, with varying percentages indicated for each form.

The potential immobilization mechanisms might be explained through the following aspects. Firstly, previous studies have shown that MPAL can effectively immobilize Cd and Pb by adsorption, ion - exchange and co-precipitation (Wu et al., 2023). The surface of MPAL contains plentiful functional groups, including -OH, -COOH, and -CH, which can provide adsorption sites for Cd and Pb (Kumpiene et al., 2008). The sulfhydryl groups of MPAL can target bind Cd and Pb to form stable CdS and PbS complexes. Secondly, C. zizanioides can immobilize abundant Cd and Pb irons by its developed root system, thus decreasing the bioavailability of Cd and Pb in soils (Rotkittikhun et al., 2007; Banerjee et al., 2016). Thirdly, the application of MPAL reduced the metal toxicity, and the enhancement of plant biomass further promoted the reduction of bioavailable Cd and Pb (Li et al., 2022).

 Effect on physiological characteristics of <italic>C. zizanioides</italic>

The effects of soil amendments on the biomass of C. zizanioides grown in Cd and Pb contaminated soils are shown in Table 1 . Compared to CK, the application of PAL and MPAL has positive effects on plant growth. Especially, in the 4% MPAL treatment, the dry weight of root, stem and leaf observably increased by 35.71%, 71.43% and 82.46%, respectively. These results indicated that MPAL could significantly promote C. zizanioides growth in the Cd and Pb contaminated soil.

The biomass of C. zizanioides under the PAL and MPAL treatments.

Treatments Fresh weight Dry weight
Roots (g) Stems (g) Leaves (g) Roots (g) Stems (g) Leaves (g)
CK 0.20 ± 0.14 a 0.61 ± 0.21 a 2.31 ± 0.05 a 0.14 ± 0.07 a 0.14 ± 0.05 a 0.57 ± 0.13 a
2% PAL 0.21 ± 0.07 a 0.60 ± 0.22 a 2.66 ± 0.99 ab 0.16 ± 0.02 a 0.15 ± 0.04 a 0.72 ± 0.15 ab
4% PAL 0.27 ± 0.06 a 0.63 ± 0.22 a 3.37 ± 0.81 ab 0.18 ± 0.04 a 0.17 ± 0.02 a 0.83 ± 0.32 ab
2% MPAL 0.23 ± 0.01 a 0.64 ± 0.07 a 3.23 ± 0.38 ab 0.16 ± 0.00 a 0.16 ± 0.01 a 0.72 ± 0.11 ab
4% MPAL 0.31 ± 0.03 a 0.73 ± 0.07 a 3.57 ± 0.10 b 0.19 ± 0.02 a 0.24 ± 0.00 b 1.04 ± 0.20 b

Values within a column followed by different lowercase letters are significantly different at p < 0.05 according to Duncan’s test.

Heavy metals can induce plants to produce superoxide anion radicals (O2·-) and hydrogen peroxide (H2O2), which are important components of reactive oxygen species (ROS) in plants. Excessive O2·- and H2O2 can lead to oxidative damage to plant tissues. In response to oxidative stress caused by heavy metals, plants produce various types of antioxidants to eliminate ROS, such as SOD, POD and CAT (Ali et al., 2013; Ben et al., 2023). Therefore, the antioxidant enzyme activities are critical protective mechanisms under heavy metal stress in plants, and the degree of oxidative stress in plants can be assessed by measuring the antioxidant enzyme activities (Luna et al., 1994; Ahmad et al., 2011; Zulfiqar et al., 2019). In this study, the production rate of O2-·, the H2O2 content, and soluble protein content increased under PAL and MPAL treatments (except for 2% PAL) ( Table 2 ). In the 4% PAL treatment, the production rate of O2·- was twice that of the CK group. The highest soluble protein contents and H2O2 content were observed in the 2% MPAL treatment, which were 15.88% and 633.33% higher than those in the CK group. Correspondingly, the application of MPAL significantly enhanced the SOD and POD activities by 8.55% - 9.12% and 29.85% - 70.14%, respectively, compared to the CK group. In addition, the activity of CAT in the 4% PAL and 4% MPAL was increased by 16.40% and 22.61%, respectively. This phenomenon showed that the addition of MPAL enhanced the antioxidant capacity of C. zizanioides under the stress of heavy metals.

The physiological characteristics of C. zizanioides under the PAL and MPAL treatments.

Treatments SOD activity (U·g-¹·FW) POD activity (U·g-¹·min-¹·FW) CAT activity (U·g-¹·min-¹·FW) Soluble protein (mg·g-¹·FW) Production rate of O2·- (nmol·g-¹·min-¹·FW) H2O2 (mmol·g-¹·FW)
CK 2138.84 ± 40.45 a 63.81 ± 6.67 a 14510 ± 632 a 25.69 ± 2.10 ab 0.11 ± 0.02 a 1467 ± 652 a
2% PAL 2122.65 ± 4.86 a 57.14 ± 5.71 a 16620 ± 1242 b 21.86 ± 2.64 a 0.12 ± 0.01 a 2119 ± 978 a
4% PAL 2051.41 ± 95.22 a 62.86 ± 0.00 a 16890 ± 1301 b 27.76 ± 2.36 b 0.22 ± 0.03 c 6237 ± 432 b
2% MPAL 2321.80 ± 31.85 b 108.57 ± 3.81 c 14685 ± 75 a 29.77 ± 1.50 b 0.21 ± 0.02 c 10758 ± 489 c
4% MPAL 2333.94 ± 104.43 b 82.86 ± 2.86 b 17790 ± 1470 b 26.75 ± 2.24 b 0.16 ± 0.02 b 6765 ± 408 b

Values within a column followed by different lowercase letters are significantly different at p < 0.05 according to Duncan’s test.

 Effect on the uptake of Cd and Pb by <italic>C. zizanioides</italic>

The uptake and accumulation of Cd and Pb by the roots, stems, and leaves of C. zizanioides are shown in Table 3 . Compared to CK, the application of PAL had no significant effect on the uptake of Cd and Pb by C. zizanioides. However, the application of MPAL observably reduced the uptake of Cd and Pb by C. zizanioides. The Cd content of roots, stems and leaves under the MPAL treatment mostly decreased by 95.06%, 54.54% and 37.55%, respectively, compared to CK group. Meanwhile, the Pb content of roots, stems and leaves decreased by 31.71% - 63.58%, 16.48% - 24.03%, and 22.35% - 23.87%, respectively.

Heavy metal concentrations (mg·kg-¹) in plant tissues under the PAL and MPAL treatments.

Treatments Cd Pb
Roots(mg·kg-¹) Stems(mg·kg-¹) Leaves(mg·kg-¹) Roots(mg·kg-¹) Stems(mg·kg-¹) Leaves(mg·kg-¹)
CK 32.41 ± 5.59 b 11.02 ± 0.07 c 5.22 ± 0.19 b 1164.05± 0.00 c 59.63 ± 4.10 ab 76.69 ± 10.20 c
2% PAL 45.97 ± 3.38 c 10.92 ± 1.32 c 6.73 ± 0.15 c 1052.55± 136.35 c 66.34 ± 12.48 b 71.73 ± 5.48 ab
4% PAL 31.61 ± 1.47 b 9.16 ± 0.87 b 3.08 ± 0.12 a 886.69± 90.26 b 62.14 ± 13.87 ab 71.60 ± 7.57 ab
2% MPAL 1.60 ± 0.07 a 5.13 ± 0.33 a 5.65 ± 0.91 b 794.95± 35.95 b 49.80 ± 2.21 ab 58.38 ± 6.19 a
4% MPAL 1.72 ± 0.14 a 5.01 ± 0.11 a 3.26 ± 0.14 a 423.95± 2.56 a 45.30 ± 8.92 a 59.55 ± 4.26 a

Values within a column followed by different lowercase letters are significantly different at p < 0.05 according to Duncan’s test.

We observed abnormally high concentrations of Cd (32.41 mg·kg-¹) and Pb (1164.05 mg·kg-¹) in the roots of the CK treatment. This phenomenon can be attributed to the relatively high background levels of Cd (3 mg·kg-¹) and Pb (700 mg·kg-¹) in the soil, together with the strong enrichment capacity of C. zizanioides. Previous studies have similarly reported excessive root accumulation of heavy metals by this species. For instance, the study by Punamiya et al. (2010) showed that in Pb-contaminated soil (800 mg·kg-¹), Pb concentration in C. zizanioides roots reached nearly 2000 mg·kg-¹. Likewise, Zhang et al. (2014) found that at a soil Cd concentration of 15 mg·kg-¹, Cd accumulation in the roots of C. zizanioides reached as high as 167 mg·kg-¹. These results consistently demonstrate the remarkable root accumulation capacity of C. zizanioides, which often exceeds the nominal soil concentrations. In our study, this characteristic explains the unusually high Cd and Pb levels observed in CK roots, confirming that the root system plays a dominant role in metal sequestration and thereby contributes to phytostabilization.

Moreover, the BCF and TF values of Cd and Pb were calculated to reveal the migration of Cd and Pb in the soil-plant system ( Table 4 ). It was observed that the BCF of Cd decreased significantly from 10.80 to 0.57 and 0.53 with the addition of 2.0% and 4.0% MPAL, respectively, and the BCF of Pb decreased from 1.66 to 0.70 with 4% MPAL addition. However, the addition of MPAL increased the TF values of Cd, but had no obvious effect on the TF values of Pb. The reduction of Cd and Pb in C. zizanioides can be explained by the decrease in bioavailable Cd and Pb in soil ( Figure 2 ). The bioavailability of Cd and Pb in soil is a vital factor to affect the uptake and translation of Cd and Pb by plants (Li et al., 2019). The MPAL application reduced the DTPA-extracted Cd and Pb and enhanced the proportion of reducible and oxidizable Cd and Pb, thereby decreasing the Cd and Pb contents in C. zizanioides.

The bioaccumulation factors (BCF) and translocation factors (TF) of C. zizanioides under the PAL and MPAL treatments.

Treatments Cd Pb
BCF TF1 TF2 BCF TF1 TF2
CK 10.80 ± 1.86 b 0.35 ± 0.05 a 0.47 ± 0.02 ab 1.66 ± 0.00 c 0.05 ± 0.00 a 1.28 ± 0.15 ab
2% PAL 15.32 ± 1.12 c 0.24 ± 0.03 a 0.62 ± 0.08 bc 1.50 ± 0.19 c 0.06 ± 0.01 a 0.93 ± 0.03 a
4% PAL 10.54 ± 0.49 b 0.29 ± 0.04 a 0.34 ± 0.04 a 1.27 ± 0.13 b 0.08 ± 0.00 b 1.18 ± 0.16 ab
2% MPAL 0.53 ± 0.02 a 3.22 ± 0.22 c 1.10 ± 0.18 d 1.14 ± 0.05 b 0.06 ± 0.00 a 1.17 ± 0.10 ab
4% MPAL 0.57 ± 0.05 a 2.93 ± 0.24 b 0.65 ± 0.04 c 0.70 ± 0.16 a 0.10 ± 0.02 c 1.37 ± 0.39 ab

Values within a column followed by different lowercase letters are significantly different at p < 0.05 according to Duncan’s test.

The observed increase in Cd TF under MPAL treatments might be attributed to two main mechanisms. First, although MPAL significantly reduced Cd content in roots, plants may regulate the activity of metal transport proteins, such as HMA2 and HMA4, to preferentially translocate the remaining Cd from roots to shoots, resulting in higher TF values. Second, MPAL may alter the speciation or binding forms of Cd in roots, making the residual Cd more readily available for xylem loading and long-distance transport to aboveground tissues (Raza et al., 2020). Together, these mechanisms explain why root Cd decreased markedly while shoot Cd was only moderately reduced, leading to an apparent increase in TF.

 Effect on soil enzyme activities

Soil enzyme activities are widely used to reflect soil biochemical properties and assess remediation efficiency (Pu and Liu, 2023). In this study, urease, catalase, cellulase, and sucrase activities were determined to estimate the biological properties of soil after the remediation with amendments and C. zizanioides ( Figure 4 ). It was observed that both amendments and C. zizanioides increased urease activity. The application of PAL and MPAL increased the urease activity by 16.71% - 19.06% and 19.32% - 31.33%, respectively, compared to CK group. The maximum urease activity was observed in the treatment of 4% MPAL with planting C. zizanioides, which was 37.70% higher than CK. Previous studies have also shown that sulfydryl modified materials application can enhance the soil urease activity. As illustrated by Zhu et al, thiourea-modified biochar significantly enhanced the urease activity (Zhu et al., 2022). However, the application of PAL and MPAL alone had no significant effects on the activities of catalase, cellulase and sucrase, but the combined application of amendments and C. zizanioides enhanced the catalase and cellulase activities. Under the treatment of PAL and MPAL, planting C. zizanioides significantly enhanced the cellulase activity by 29.03% - 110.22% compared to CK. In addition, the application of 4% MPAL and C. zizanioides significantly increased the sucrase activity by 42.99%. The soil enzyme was secreted from plants and soil microorganisms. The MPAL application reduced the soil Cd and Pb bioavailability, and promoted C. zizanioides growth. The rhizosphere activity of C. zizanioides might recruit more microorganisms and enhance the enzyme activities.

Soil urease activity (a), sucrase activity (b), cellulase activity (c) and catalase activity (d) under different treatments. Mean with different letters indicated significant difference from each other according to one-way ANOVA and Duncan’s test (p < 0.05).

Four bar charts compare soil enzyme activities under different treatments for unplanted and planted vetiver. Chart a shows urease activity, chart b shows sucrase activity, chart c shows soil cellulase activity, and chart d shows soil catalase activity. Each chart indicates variation in enzyme activities across six treatments, with labels indicating significant differences. Effects on soil bacterial community

In this study, the effects of soil amendments and the plant C. zizanioides on bacterial community structure were analyzed using 16S rRNA gene sequencing. The richness and diversity of bacterial communities at the OTU level under different treatments are shown in Supplementary Table S1 . The Ace and Chao indices reflect the richness of bacterial communities, while the Shannon and Simpson indices are used to evaluate their diversity (Wu et al., 2018). No significant variation in all indices was observed after the application of PAL and MPAL, indicating that their application has little negative impact on bacterial diversity. However, the C. zizanioides planting enhanced the bacterial Chao, Ace and Shannon indexes. The growth of C. zizanioides can recruit more beneficial microorganisms by root exudates.

Figures 5a, b shows that all treatments have a similar community composition at the phylum level. The dominant bacterial phyla were detected to be Actinobacteriota, Proteobacteria, Chloroflexi and Acidobacteriota, accounting for 78.62% - 85.56% of the total sequences. Actinobacteriota is an important phylum for metal remediation due to its function of metabolizing and promoting the absorption of heavy metals by plants (Alvarez et al., 2017; Narendrula-Kotha and Nkongolo, 2017). Chloroflexi can better adapt to heavy metal stress and participate in the metabolism of organic matter and the transformation of pollutants (Gupta et al., 2023). In the treatment of cultivation of C. zizanioides, the addition of PAL and MPAL increased the abundance of Actinobacteriota and Chloroflexi. Moreover, the abundance of Actinobacteriota and Chloroflexi is negatively correlated with the concentrations of heavy metals (Li et al., 2022; Wu et al., 2023), indicating that the incorporation of amendment reduces the toxicity of heavy metals and enhances soil microecology. Acidobacteriota was adapted to low pH condition (Qin et al., 2020), and it might be the reason why cultivation of C. zizanioides significantly increased the abundance of Acidobacteriota compared to the control. Other phyla were relatively stable in all treatments.

Community abundance on phylum level (a, b) and genus level (c, d) under different treatments. The structural equation model analysis between environmental factors and bacterial richness, numbers next to the arrows indicate standardized path coefficients and asterisks mark their significance: *<0.05; **<0.01; or ***<0.001 (e).

Four bar charts labeled a to d show the percent of community abundance at the phylum and genus levels across different treatments, with each segment representing different bacterial groups. The bottom diagram, labeled e, is a path analysis model showing relationships between DTPA-Cd, DTPA-Pb, and soil enzyme activities like catalase and cellulase, with bacterial richness. Positive and negative relationships are indicated by green and red lines, respectively, with significance levels noted.

Figures 5c, d showed that the community composition at the genus level is significantly different. In the cultivation of C. zizanioides alone, Vicinamibacterales, Vicinamibacteraceae and Gemmatimonadaceae were dominant in the soil. As illustrated by Kou et al, Vicinamibacterales and Vicinamibacteraceae may be resistant to heavy metals, which may be the reason for this phenomenon (Kou et al., 2023). The addition of PAL and MPAL significantly decreased the abundance of Vicinamibacteraceae and Gemmatimonadaceae. We speculated that the application of amendments decreased the bioavailability of heavy metals in the soil could potentially account for this result. Sphingomonas is a gram-negative bacteria, which was sensitive to metal contamination stress (Wang et al., 2023). Without C. zizanioides planting, Sphingomonas was the dominant bacterial genus, after C. zizanioides planting, the abundance of Sphingomonas decreased slightly, indicating that the application of C. zizanioides reduces the bioavailability of heavy metals in soil.

The Structural Equation Model (SEM) was constructed to examine the causal relationships among DTPA-extractable Cd and Pb, soil enzyme activities (urease, catalase, cellulase), and bacterial richness (Sobs) ( Figure 5e ). The results indicated that heavy metals directly suppressed bacterial richness and indirectly influenced it through negative effects on soil enzyme activities. The SEM showed excellent fit (χ²/df = 0.58, CFI = 1.00, RMSEA = 0.00), confirming that the proposed pathways accurately reflect the observed relationships. Compared with simple correlation analysis, SEM provides a powerful tool that can distinguish the direct and indirect effects of multiple factors on contaminated soil (Li et al., 2025). Our research results indicate that the toxicity of Cd and Pb reduces the activity of soil enzymes, thereby restricting the turnover of organic matter and the cycling of nutrients, and ultimately inhibiting the diversity of microorganisms. Importantly, the MPAL treatment alleviates these negative effects by reducing the bioavailability of metals, demonstrating its direct and indirect benefits for the soil microecology. These findings emphasize that MPAL-assisted plant stabilization not only can fix metals but also can help maintain the functional connection between soil enzymes and microbial communities.

 Conclusions

This study demonstrates that the application of mercapto-functionalized palygorskite (MPAL) effectively enhances the phytostabilization capacity of Chrysopogon zizanioides for Cd and Pb. The addition of MPAL significantly reduced the bioavailable fractions of Cd and Pb in the soil, with the 4% MPAL treatment cultivated with C. zizanioides decreasing DTPA-extractable Cd and Pb by 89.22% and 51.18%, respectively. Meanwhile, MPAL markedly promoted plant growth and improved soil biological properties, including enhanced soil enzyme activities and microbial richness. These findings indicate that MPAL-assisted phytostabilization is a promising strategy for the remediation of soils co-contaminated with Cd and Pb. However, this study was conducted under pot-scale conditions with artificially contaminated soils, which may not fully represent the complexity of field environments in mining areas. Future research should focus on field-scale validation to assess the long-term stability and remediation efficiency of MPAL-assisted phytostabilization, as well as its applicability under multi-metal co-contamination conditions.

 Data availability statement

The original contributions presented in the study are publicly available. This data can be found at the National Center for Biotechnology Information (NCBI) using accession number PRJNA1284549.

 Author contributions

HK: Data curation, Investigation, Writing – original draft. BW: Funding acquisition, Project administration, Supervision, Writing – review & editing. FP: Data curation, Investigation, Writing – original draft. YY: Methodology, Visualization, Writing – original draft. XM: Conceptualization, Writing – original draft. YS: Funding acquisition, Writing – review & editing.

 Funding

The author(s) declare financial support was received for the research and/or publication of this article. This study was financially supported by National Natural Science Foundation of China (U24A20622), Science and Technology Major Project of Tibetan Autonomous Region of China (XZ202201ZD0004G06), Central Government Guides Local Science and Technology Development Project of Tibetan Autonomous Region of China (XZ202401YD0001), Guizhou Provincial Key Technology R&D Program (No.QKHZC(2023)YB255), the Science and Technology Program of Sichuan Province (2022YFN0066), and State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Independent Research Project (grant no. SKLGP2021Z030), Sichuan Science and Technology Program (2023YFS0483). Chengdu Science and Technology Project (2024-YF09-00042-SN).

 Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Generative AI statement

The author(s) declare that no Generative AI was used in the creation of this manuscript.

Any alternative text (alt text) provided alongside figures in this article has been generated by Frontiers with the support of artificial intelligence and reasonable efforts have been made to ensure accuracy, including review by the authors wherever possible. If you identify any issues, please contact us.

 Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

 Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2025.1659418/full#supplementary-material

References Ahmad P. Nabi G. Ashraf M. (2011). Cadmium-induced oxidative damage in mustard [Brassica juncea (L.) Czern. & Coss.] plants can be alleviated by salicylic acid. South Afr. J. Bot. 77, 36–44. doi: 10.1016/j.sajb.2010.05.003 Ai Y. Wang Y. Song L. Hong W. Zhang Z. Li X. . (2023). Effects of biochar on the physiology and heavy metal enrichment of Vetiveria zizanioides in contaminated soil in mining areas. J. Hazardous Materials 448, 130965. doi: 10.1016/j.jhazmat.2023.130965, PMID: 36860049 Alam O. Zheng X. J. Du D. L. Qiao X. C. Dai L. Y. Li J. . (2024). A critical review on advances in remediation of toxic heavy metals contaminated solids by chemical processes. J. Environ. Chem. Eng. 12, 113149. doi: 10.1016/j.jece.2024.113149 Ali H. Khan E. Sajad M. A. (2013). Phytoremediation of heavy metals—Concepts and applications. Chemosphere 91, 869–881. doi: 10.1016/j.chemosphere.2013.01.075, PMID: 23466085 Alvarez A. Saez J. M. Davila Costa J. S. Colin V. L. Fuentes M. S. Cuozzo S. A. . (2017). Actinobacteria: Current research and perspectives for bioremediation of pesticides and heavy metals. Chemosphere 166, 41–62. doi: 10.1016/j.chemosphere.2016.09.070, PMID: 27684437 Antoniadis V. Shaheen S. M. Levizou E. Shahid M. Niazi N. K. Vithanage M. . (2019). A critical prospective analysis of the potential toxicity of trace element regulation limits in soils worldwide: Are they protective concerning health risk assessment? - A review. Environ. Int. 127, 819–847. doi: 10.1016/j.envint.2019.03.039, PMID: 31051325 Azeez L. Adejumo A. L. Lateef A. Adebisi S. A. Adetoro R. O. Adewuyi S. O. . (2019). Zero-valent silver nanoparticles attenuate Cd and Pb toxicities on Moringa oleifera via immobilization and induction of phytochemicals. Plant Physiol. Biochem. 139, 283–292. doi: 10.1016/j.plaphy.2019.03.030, PMID: 30925438 Baker A. J. M. McGrath S. P. Sidoli C. M. D. Reeves R. D. (1994). The possibility of in situ heavy metal decontamination of polluted soils using crops of metal-accumulating plants. Resources Conserv. Recycling 11, 41–49. doi: 10.1016/0921-3449(94)90077-9 Banerjee R. Goswami P. Pathak K. Mukherjee A. (2016). Vetiver grass: An environment clean-up tool for heavy metal contaminated iron ore mine-soil. Ecol. Eng. 90, 25–34. doi: 10.1016/j.ecoleng.2016.01.027 Ben Y. Cheng M. Liu Y. Wang X. Wang L. Yang Q. . (2023). Biomarker changes and oxidative damage in living plant cells as new biomonitoring indicators for combined heavy metal stress assessment. Ecol. Indic. 154, 110784. doi: 10.1016/j.ecolind.2023.110784 Bradford M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. doi: 10.1006/abio.1976.9999, PMID: 942051 Cuypers A. Plusquin M. Remans T. Jozefczak M. Keunen E. Gielen H. . (2010). Cadmium stress: an oxidative challenge. BioMetals 23, 927–940. doi: 10.1007/s10534-010-9329-x, PMID: 20361350 Durak I. Yurtarslanl Z. Canbolat O. Akyol Ö. (1993). A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clinica Chimica Acta 214, 103–104. doi: 10.1016/0009-8981(93)90307-P, PMID: 8453769 Edelstein M. Plaut Z. Dudai N. Ben-Hur M. (2009). Vetiver (Vetiveria zizanioides) responses to fertilization and salinity under irrigation conditions. J. Environ. Manage. 91, 215–221. doi: 10.1016/j.jenvman.2009.08.006, PMID: 19709801 El-Sharkawy M. Li J. Kamal N. Mahmoud E. Omara A. E. D. Du D. L. (2023). Assessing and predicting soil quality in heavy metal-contaminated soils: statistical and ANN-based techniques. J. Soil Sci Plant Nutr. 23, 6510–6526. doi: 10.1007/s42729-023-01507-w Fu C. Zhu X. Dong X. Zhao P. Wang Z. (2021). Study of adsorption property and mechanism of lead(II) and cadmium(II) onto sulfhydryl modified attapulgite. Arabian J. Chem. 14, 102960. doi: 10.1016/j.arabjc.2020.102960 George S. Manoharan D. Li J. Britton M. Parida A. (2017). Drought and salt stress in Chrysopogon zizanioides leads to common and specific transcriptomic responses and may affect essential oil composition and benzylisoquinoline alkaloids metabolism. Curr. Plant Biol. 11-12, 12–22. doi: 10.1016/j.cpb.2017.12.001 Gravand F. Rahnavard A. Pour G. M. J. S. Journal S. C. A. I. (2020). Investigation of vetiver grass capability in phytoremediation of contaminated soils with heavy metals (Pb, cd, mn, and ni). Soil and Sediment Contamination: An International Journal 30, 163–186. doi: 10.1080/15320383.2020.1819959 Gupta S. Graham D. W. Sreekrishnan T. R. Ahammad S. Z. (2023). Exploring the impacts of physicochemical characteristics and heavy metals fractions on bacterial communities in four rivers. J. Environ. Manage. 325, 116453. doi: 10.1016/j.jenvman.2022.116453, PMID: 36274305 Huang Y. Hu Y. Liu Y. (2009). Heavy metal accumulation in iron plaque and growth of rice plants upon exposure to single and combined contamination by copper, cadmium and lead. Acta Ecologica Sin. 29, 320–326. doi: 10.1016/j.chnaes.2009.09.011 Jiang Y. Hu T. Peng O. Chen A. Tie B. Shao J. (2021). Responses of microbial community and soil enzyme to heavy metal passivators in cadmium contaminated paddy soils: An in situ field experiment. Int. Biodeterioration Biodegradation 164, 105292. doi: 10.1016/j.ibiod.2021.105292 Jiang Y. Yi X.-T. Liu M.-Y. Liu B.-B. Zhou H. Zeng P. . (2022). Dynamic responses of soil enzymes at key growth stages in rice after the in situ remediation of paddy soil contaminated with cadmium and arsenic. Sci Total Environ. 830, 154633. doi: 10.1016/j.scitotenv.2022.154633, PMID: 35314228 Kama R. Liu Y. Aidara M. Kpalari D. F. Song J. Diatta S. . (2024). Plant-soil feedback combined with straw incorporation under maize/soybean intercropping increases heavy metals migration in soil-plant system and soil HMRG abundance under livestock wastewater irrigation. J. Soil Sci Plant Nutr. 24, 7090–7104. doi: 10.1007/s42729-024-02026-y Kolahi M. Mohajel Kazemi E. Yazdi M. Goldson-Barnaby A. (2020). Oxidative stress induced by cadmium in lettuce (Lactuca sativa Linn.): Oxidative stress indicators and prediction of their genes. Plant Physiol. Biochem. 146, 71–89. doi: 10.1016/j.plaphy.2019.10.032, PMID: 31734520 Kou B. He Y. Wang Y. Qu C. Tang J. Wu Y. . (2023). The relationships between heavy metals and bacterial communities in a coal gangue site. Environ. pollut. 322, 121136. doi: 10.1016/j.envpol.2023.121136, PMID: 36736561 Kumpiene J. Lagerkvist A. Maurice C. (2008). Stabilization of As, Cr, Cu, Pb and Zn in soil using amendments – A review. Waste Manage. 28, 215–225. doi: 10.1016/j.wasman.2006.12.012, PMID: 17320367 Li J. Wu B. Luo Z. Lei N. Kuang H. Li Z. (2022). Immobilization of cadmium by mercapto-functionalized palygorskite under stimulated acid rain: Stability performance and micro-ecological response. Environ. pollut. 306, 119400. doi: 10.1016/j.envpol.2022.119400, PMID: 35525516 Li X. Zhang X. Wang X. Cui Z. (2019). Phytoremediation of multi-metal contaminated mine tailings with Solanum nigrum L. and biochar/attapulgite amendments. Ecotoxicology Environ. Saf. 180, 517–525. doi: 10.1016/j.ecoenv.2019.05.033, PMID: 31128549 Li D. Zhang X. Zhang H. Fan Q. Guo B. Li J. (2025). A global meta-analysis reveals effects of heavy metals on soil microorganisms. J. Hazardous Materials 491, 138018. doi: 10.1016/j.jhazmat.2025.138018, PMID: 40138950 Liao J. (2015). Analysis of peroxidase activity of wheat seed. Chin. Agric. Sci. Bull. 31, 123–129. doi: 10.11924/j.issn.1000-6850.casb15030025 Liu J. Liu Y. Habeebu S. M. Waalkes M. P. Klaassen C. D. (2000). Chronic combined exposure to cadmium and arsenic exacerbates nephrotoxicity, particularly in metallothionein-I/II null mice. Toxicology 147, 157–166. doi: 10.1016/S0300-483X(00)00194-3, PMID: 10924798 Lu X. Sun J. Pan G. Qi W. Zhang Z. Xing J. . (2025). Ball-milling-modified biochar with additives enhances soil cd passivation, increases plant growth and restrains cd uptake by chinese cabbage. Horticulturae. 11, 168. doi: 10.3390/horticulturae11020168 Luna C. M. González C. A. Trippi V. S. J. P. Physiology C. (1994). Oxidative damage caused by an excess of copper in oat leaves. Plant Cell Physiol. 35, 11–15. doi: 10.1093/oxfordjournals.pcp.a078561 Madrid F. Liphadzi M. S. Kirkham M. B. (2008). “Chapter 29 EDTA-assisted phytostabilization by barley roots contaminated with heavy metals,” in Developments in Soil Science. Eds. Hartemink A. E. McBratney A. B. Naidu R. (Amsterdam: Elsevier), 697–718. doi: 10.1016/S0166-2481(07)32029-1 Marschner H. Römheld V. Cakmak I. (1987). Root-induced changes of nutrient availability in the rhizosphere. J. Plant Nutr. 10, 1175–1184. doi: 10.1080/01904168709363645 Masinire F. Adenuga D. O. Tichapondwa S. M. Chirwa E. M. N. (2021). Phytoremediation of Cr(VI) in wastewater using the vetiver grass (Chrysopogon zizanioides). Minerals Eng. 172, 107141. doi: 10.1016/j.mineng.2021.107141 Narendrula-Kotha R. Nkongolo K. K. (2017). Bacterial and fungal community structure and diversity in a mining region under long-term metal exposure revealed by metagenomics sequencing. Ecol. Genet. Genomics 2, 13–24. doi: 10.1016/j.egg.2016.11.001 Naz M. Dai Z. Hussain S. Tariq M. Danish S. Khan I. U. . (2022). The soil pH and heavy metals revealed their impact on soil microbial community. J. Environ. Manage. 321, 115770. doi: 10.1016/j.jenvman.2022.115770, PMID: 36104873 Pandey V. C. Praveen A. (2020). “2 - Vetiveria zizanioides (L.) Nash – more than a promising crop in phytoremediation,” in Phytoremediation Potential of Perennial Grasses. Eds. Pandey V. C. Singh D. P. (Amsterdam: Elsevier), 31–62. doi: 10.1016/B978-0-12-817732-7.00002-X Pidatala V. R. Li K. Sarkar D. Wusirika R. Datta R. (2018). Comparative metabolic profiling of vetiver (Chrysopogon zizanioides) and maize (Zea mays) under lead stress. Chemosphere 193, 903–911. doi: 10.1016/j.chemosphere.2017.11.087, PMID: 29874765 Pu S. Liu S. (2023). “Chapter 5 - Effect of biomass-based materials on enzyme activities in heavy metal-contaminated environment,” in Extracellular Enzymes in Environments. Eds. Pu S. Liu S. (Amsterdam: Elsevier), 241–386. doi: 10.1016/B978-0-443-21772-2.00005-6 Punamiya P. Datta R. Sarkar D. Barber S. Patel M. Das P. (2010). Symbiotic role of Glomus mosseae in phytoextraction of lead in vetiver grass [Chrysopogon zizanioides (L.). J. Hazardous Materials 177, 465–474. doi: 10.1016/j.jhazmat.2009.12.056, PMID: 20061082 Qin C. Yuan X. Xiong T. Tan Y. Z. Wang H. (2020). Physicochemical properties, metal availability and bacterial community structure in heavy metal-polluted soil remediated by montmorillonite-based amendments. Chemosphere 261, 128010. doi: 10.1016/j.chemosphere.2020.128010, PMID: 33113657 Raza A. Habib M. Kakavand S. N. Zahid Z. Zahra N. Sharif R. . (2020). Phytoremediation of cadmium: physiological, biochemical, and molecular mechanisms. Biol. (Basel) 9, 177. doi: 10.3390/biology9070177, PMID: 32708065 Rotkittikhun P. Chaiyarat R. Kruatrachue M. Pokethitiyook P. Baker A. J. M. (2007). Growth and lead accumulation by the grasses Vetiveria zizanioides and Thysanolaena maxima in lead-contaminated soil amended with pig manure and fertilizer: A glasshouse study. Chemosphere 66, 45–53. doi: 10.1016/j.chemosphere.2006.05.038, PMID: 16828842 Ruttens A. Adriaensen K. Meers E. De Vocht A. Geebelen W. Carleer R. . (2010). Long-term sustainability of metal immobilization by soil amendments: Cyclonic ashes versus lime addition. Environ. pollut. 158, 1428–1434. doi: 10.1016/j.envpol.2009.12.037, PMID: 20080327 Setu S. Strezov V. (2025). Impacts of non-ferrous metal mining on soil heavy metal pollution and risk assessment. Sci Total Environ. 969, 178962. doi: 10.1016/j.scitotenv.2025.178962, PMID: 40022981 Shackira A. M. Puthur J. T. (2019). “Phytostabilization of Heavy Metals: Understanding of Principles and Practices,” in Plant-Metal Interactions. Eds. Srivastava S. Srivastava A. K. Suprasanna P. (Springer International Publishing, Cham), 263–282. Shen X. Dai M. Yang J. Sun L. Tan X. Peng C. . (2022). A critical review on the phytoremediation of heavy metals from environment: Performance and challenges. Chemosphere 291, 132979. doi: 10.1016/j.chemosphere.2021.132979, PMID: 34801572 Song J. Brookes P. C. Shan S. Xu J. Liu X. (2022). Effects of remediation agents on microbial community structure and function in soil aggregates contaminated with heavy metals. Geoderma 425, 116030. doi: 10.1016/j.geoderma.2022.116030 Uchida A. Jagendorf A. T. Hibino T. Takabe T. Takabe T. (2002). Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Sci 163, 515–523. doi: 10.1016/S0168-9452(02)00159-0 Wang X. Dai Z. Lin J. Zhao H. Yu H. Ma B. . (2023). Heavy metal contamination collapses trophic interactions in the soil microbial food web via bottom-up regulation. Soil Biol. Biochem. 184, 109058. doi: 10.1016/j.soilbio.2023.109058 Wang B. Wang C. Li J. Sun H. Xu Z. (2014). Remediation of alkaline soil with heavy metal contamination using tourmaline as a novel amendment. J. Environ. Chem. Eng. 2, 1281–1286. doi: 10.1016/j.jece.2014.05.017 Wu B. Hou S. Peng D. Wang Y. Wang C. Xu F. . (2018). Response of soil micro-ecology to different levels of cadmium in alkaline soil. Ecotoxicology Environ. Saf. 166, 116–122. doi: 10.1016/j.ecoenv.2018.09.076, PMID: 30253286 Wu B. Li J. Kuang H. Shangguan Y. Chen J. (2023). Mercapto-based palygorskite modified soil micro-biology and reduced the uptake of heavy metals by Salvia miltiorrhiza in cadmium and lead co-contaminated soil. J. Environ. Manage. 345, 118859. doi: 10.1016/j.jenvman.2023.118859, PMID: 37647730 Yang Z. Gong H. He F. Repo E. Yang W. Liao Q. . (2022b). Iron-doped hydroxyapatite for the simultaneous remediation of lead-, cadmium- and arsenic-co-contaminated soil. Environ. pollut. 312, 119953. doi: 10.1016/j.envpol.2022.119953, PMID: 36028081 Yang H. Wang M. Chen X. Xu Y. Zong L. Huang Q. . (2022a). Sulfhydryl grafted palygorskite amendment with varying loading rates: Characteristic differences and dose-effect relationship for immobilizing soil Cd. Sci Total Environ. 842, 156926. doi: 10.1016/j.scitotenv.2022.156926, PMID: 35753494 Yong Y. Xu Y. Huang Q. Sun Y. Wang L. Liang X. . (2022). Remediation effect of mercapto-palygorskite combined with manganese sulfate on cadmium contaminated alkaline soil and cadmium accumulation in pak choi (Brassica chinensis L.). Sci Total Environ. 813, 152636. doi: 10.1016/j.scitotenv.2021.152636, PMID: 34963609 Yong Y. Yang T. Wang Y. Xu Y. Huang Q. Liang X. . (2024). Mercapto–palygorskite decreases the Cd uptake of wheat by changing Fe and Mn fraction in Cd contaminated alkaline soil. Geoderma 441, 116751. doi: 10.1016/j.geoderma.2023.116751 Zeng F. Ali S. Zhang H. Ouyang Y. Qiu B. Wu F. . (2011). The influence of pH and organic matter content in paddy soil on heavy metal availability and their uptake by rice plants. Environ. pollut. 159, 84–91. doi: 10.1016/j.envpol.2010.09.019, PMID: 20952112 Zhan J. Huang H. Yu H. Zhang X. Wang Y. Li T. (2020). Characterization of dissolved organic matter in the rhizosphere of phytostabilizer Athyrium wardii (Hook.) involved in enhanced metal accumulation when exposed to Cd and Pb co-contamination. Environ. pollut. 266, 115196. doi: 10.1016/j.envpol.2020.115196, PMID: 32771840 Zhang X. Gao B. Xia H. (2014). Effect of cadmium on growth, photosynthesis, mineral nutrition and metal accumulation of bana grass and vetiver grass. Ecotoxicology Environ. Saf. 106, 102–108. doi: 10.1016/j.ecoenv.2014.04.025, PMID: 24836884 Zheng R. Feng X. Zou W. Wang R. Yang D. Wei W. . (2021). Converting loess into zeolite for heavy metal polluted soil remediation based on “soil for soil-remediation“ strategy. J. Hazardous Materials 412, 125199. doi: 10.1016/j.jhazmat.2021.125199, PMID: 33556854 Zheng X. J. Lin H. J. Du D. L. Li G. L. Alam O. Cheng Z. . (2024). Remediation of heavy metals polluted soil environment: A critical review on biological approaches. Ecotoxicol. Environ. Saf. 284, 116883. doi: 10.1016/j.ecoenv.2024.116883, PMID: 39173222 Zhou Y. Lan W. Yang F. Zhou Q. Liu M. Li J. . (2024). Invasive Amaranthus spp. for heavy metal phytoremediation: Investigations of cadmium and lead accumulation and soil microbial community in three zinc mining areas. Ecotoxicology Environ. Saf. 285, 117040. doi: 10.1016/j.ecoenv.2024.117040, PMID: 39270476 Zhu Y. Ma J. Chen F. Yu R. Hu G. Yang Y. (2022). Effective alleviation of Cd stress to microbial communities in mining reclamation soils by thiourea-modified biochar amendment. Pedosphere 32, 866–875. doi: 10.1016/j.pedsph.2022.06.017 Zulfiqar U. Farooq M. Hussain S. Maqsood M. Hussain M. Ishfaq M. . (2019). Lead toxicity in plants: Impacts and remediation. J. Environ. Manage. 250, 109557. doi: 10.1016/j.jenvman.2019.109557, PMID: 31545179