Front. Plant Sci. Frontiers in Plant Science Front. Plant Sci. 1664-462X Frontiers Media S.A. 10.3389/fpls.2024.1361202 Plant Science Original Research Response of cotton growth, yield, and water and nitrogen use efficiency to nitrogen application rate and ionized brackish water irrigation under film-mulched drip fertigation Wei Kai Wang Quanjiu * Deng Mingjiang Lin Shudong Guo Yi State Key Laboratory of Eco-hydraulics in Northwest Arid Region, Xi’an University of Technology, Xi’an, China

Edited by: Ivan A. Paponov, Aarhus University, Denmark

Reviewed by: Rosario Alvarez, Sevilla University, Spain

Alessandra Moncada, University of Palermo, Italy

*Correspondence: Quanjiu Wang, wquanjiu@163.com

28 03 2024 2024 15 1361202 25 12 2023 13 03 2024 Copyright © 2024 Wei, Wang, Deng, Lin and Guo 2024 Wei, Wang, Deng, Lin and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

 Introduction

The presence of brackish water resources is significant in addressing the scarcity of freshwater resources, particularly in the Xinjiang region. Studies focused on reducing adverse effect of brackish water irrigation based on using ionized brackish water, as well as on investigating its effects on fibre and oil plant production processes, remain incipient in the literature. Some benefits of this technique are the optimization of the quality and quantity of irrigation water, economy of water absorbed by the plants, improvement in the vegetative growth and productivity compared to irrigation using conventional brackish water. Thus, the aim of the current study is to assess the effect of different nitrogen application rates on soil water and salinity, cotton growth and water and nitrogen use efficiency.

 Methods

The experimental design consisted of completely randomized design with two water types (ionized and non-ionized) and six nitrogen application rates with four replications.

 Results

Irrigation conducted with ionized brackish water and different nitrogen application rates had significant effect on the plant height, leaf area index, shoot dry matter, boll number per plant and chlorophyll content. The study also demonstrated significant effects of ionized brackish water on soil water content and soil salinity accumulation. The highest cotton production was achieved with the use of 350 kg·ha-1 of ionized brackish water for irrigation, resulting in an average increase of 11.5% compared to the use of non-ionized brackish water. The nitrogen application exhibits a quadratic relationship with nitrogen agronomic use efficiency and apparent nitrogen use efficiency, while it shows a liner relationship with nitrogen physiological use efficiency and nitrogen partial productivity. After taking into account soil salinity, cotton yield, water and nitrogen use efficiency, the optimal nitrogen application rate for ionized brackish water was determined to be 300 kg·ha -1 .

 Discussion

It is hoped that this study can contribute to improving water management, reducing the environmental impact without implying great costs for the producer.

 cotton ionized brackish water plant height shoot dry matter water-nitrogen use efficiency 52339003, 41830754, 51679190 National Natural Science Foundation of China10.13039/501100001809 section-in-acceptance Crop and Product Physiology Introduction

With the increasing global population and improving living standards, people’s demand for agricultural products has increased dramatically (Ren et al., 2019, 2022). However, agricultural production requires many materials, especially fertilizer input, which leads to the continuous reduction of available resources and the deterioration of the ecological environment (Gong et al., 2011; Fan et al., 2019). Therefore, it is a major challenge to obtain a higher yield at a lower cost (including economically and environmentally). Low fertilizer inputs are crucial for efficient resource utilization, cleaner crop production, and achieving the goal of peak carbon emission and carbon neutrality (Chen et al., 2014; Gerten et al., 2020; Van Dijk et al., 2021).

Agriculture has become the principal freshwater consumer, accounting for 70% of the global freshwater withdrawals (Foley et al., 2011). In the face of the population growth and good-quality water scarcity (Rodell et al., 2018), a lot of scientists recommend an irrigation with marginal water, i.e., brackish or saline water, for alleviating the pressure imposed from agricultural production on water needs (Skaggs et al., 2014; Assouline et al., 2015; Li and Ren, 2021). In the recent three decades, brackish water has been increasingly applied in many regions, especially in arid and semi-arid areas (Mehta et al., 2000; Yang et al., 2020). Brackish water irrigation can result in salt stress to plants, physiological drought, reduced soil oxygen content, anaerobic respiration by roots, and accumulation of toxic substances (Bouksila et al., 2013). Excess salt affects root water absorption, photosynthesis, and transpiration. This leads to growth inhibition of roots, stems, leaves, and other organs and reduces dry matter production, ultimately leading to a reduction in crop yield (Parida et al., 2004). Owing to the adverse effects on soil properties and plant growth and productivity by irrigation with brackish water, the search continues for more efficient irrigation methods that minimize waste, reduce salt stress, and maintain crop productivity. Ionized water treatment has shown promising potential in saving water resource and promoting agricultural productivity that will be of significant importance in the near future. Wang et al. (2016) reported the ionized treatment of water decreases water surface tension. A reduction in surface tension can enhance the hydration ability of water molecules and ions as well as the mineral salt dissolving capacity. Zhao et al. (2021) demonstrated that irrigation with ionized fresh water improves plant height and yield of winter wheat. Thus, the ionized water treatment can lead to improvements in terms of cleaner and sustainable production.

Cotton (Gossypium hirsutum L.) is the most important oil and fiber crop worldwide (Li et al., 2022). Xinjiang, China, is the largest irrigated cotton-producing arid region in the world. It contributed more than 19% to the global production from only 7.8% of the worldwide cotton sown area (Shi et al., 2022). Different nitrogen application rates will affect cotton’s nitrogen absorption (Sun et al., 2023). The nitrogen fertilizer application rate will significantly affect the plant height, main stem nodes, number of bolls per plant, boll weight, and seed cotton yield (Munir et al., 2015). Reductions in growth combined with increased fruit shed under severe N deficiency lead to a decrease in final boll number per plant and end-of-season lint yield (Bondada and Oosterhuis, 2001). Conversely, high N application rates can produce excessive vegetative growth, poor fruit retention at lower mainstem nodes, and delayed crop maturity (Boquet and Breitenbeck, 2000). It was also reported that nitrogen fertilization improves the salinity tolerance of cotton plants, because N played both nutritional and osmotic roles in saline conditions (Ding et al., 2010). However, N management in cotton is particularly difficult due to problems with either excessive or inadequate rates or influence of abiotic stresses like drought of salinity (Rinehardt et al., 2004; Dong et al., 2012). In recent years, fertilizer management in salt-affected cotton fields has attracted a number of interests. Chen et al. (2010) studied the influence of different N fertilization rates and soil salinity levels on the growth and nitrogen uptake of potted cotton plants. They found that cotton growth was significantly affected by the interaction of soil salinity and N but not by N alone. When using saline water for irrigation, excessive nitrogen application would cause more alkaline cations in the soils, increase soil salinity, and inhibit the absorption of nitrogen by roots and thus reduce crop yields (Han et al., 2015). Proper management of N fertilizer is especially important in saline soils where N application might reduce the adverse effects of salinity on plant growth and yield (Hou et al., 2009).

Because of the arid conditions and limited water resources in southern Xinjiang, where much of the region is situated on the desert periphery, saline groundwater (with concentrations ranging from 1 g·L−1 to 12 g·L−1) was extensively employed as a substitute for fresh water in cotton cultivation. We hypothesize that the use of water treated with an ionized system can increase cotton growth, improve water use efficiency (WUE) and nitrogen use efficiency (NUE), and reduce soil salinity accumulation. However, there is little information available in the literature about cotton irrigation with ionized treated water. Thus, the aim of the current study was to investigate the effect of different nitrogen application rates on soil water and salinity, cotton growth, and WUE and NUE under ionized and non-ionized brackish water irrigation.

 Materials and methods Experimental site and cultivar

The 3-year (2017, 2018, and 2019) field trials were conducted at the Experiment Station of Bazhou Irrigation (41°45’20.24”N, 86°8’51.16”E; altitude 901 m) in Korla City, Xinjiang Province, Northwest China. The physical characteristics of the soil layer from 0 to 100 cm are depicted in Table 1 . According to USDA-NRCS (2020), the soil in the years 2017 and 2018 was categorized as sandy loam. It had a pH level of 8.75, a total organic matter content of 76.8 mg·kg−1, a total nitrogen content of 3.92 mg·kg−1, an available phosphorus content of 31.1 mg·kg−1, and an available potassium content of 72.0 mg·kg−1. In 2019, the soil was categorized as sandy with a pH level of 8.82. It contained 89.1 mg·kg−1 of total organic matter, 4.87 mg·kg−1 of total nitrogen, 30.8 mg·kg−1 of available phosphorus, and 79.5 mg·kg−1 of available potassium. The groundwater depth is below 7 m. The electronic conductivity (EC) of groundwater is 2.73–2.95 ms·cm−1. Table 2 provides the chemical characteristics of groundwater, with a total dissolved solids concentration of 2.2 g·L−1. An automatic weather station was installed in the experimental area to record the daily climatic information, which encompassed precipitation, air temperature, solar radiation, wind speed, relative humidity, and air pressure. According to the weather data, the seasonal precipitation during 2017, 2018, and 2019 cotton seasons was 64.4, 49.8, and 19 mm, respectively. Figure 1 displays the daily values for the mean air temperature, lowest air temperature, and maximum air temperature, highest air temperature, and rainfall for cotton seasons.

Physical properties of the soil in the experimental site.

Soil layer (cm) Soil texture Soil texture Bulk density (g cm−3) θ WP (cm3 cm-3) θ FC (cm3 cm-3) θ s (cm3 cm-3)
Sand (%) Silt (%) Clay (%)
2017 2018 0–20 43.2 52.8 4.0 Silt loam 1.52 0.0309 0.206 0.3319
20–40 64.0 33.1 2.9 Sandy loam 1.52 0.0297 0.288 0.3502
40–60 64.5 32.6 2.9 Sandy loam 1.54 0.0296 0.211 0.3466
60–80 73.9 24.0 2.1 Loamy sand 1.52 0.0328 0.233 0.3630
80–100 83.6 15.1 1.3 Loamy sand 1.63 0.0379 0.214 0.3425
2019 0–20 83.1 15.3 1.6 Loamy sand 1.47 0.0391 0.250 0.3862
20–40 89.5 9.7 0.8 Sandy 1.64 0.0426 0.170 0.3412
40–60 88.6 10.5 0.9 Sandy 1.54 0.0430 0.198 0.3699
60–80 85.0 13.3 1.7 Loamy sand 1.53 0.0406 0.207 0.3721
80–100 82.0 16.2 1.8 Loamy sand 1.55 0.0380 0.212 0.3643

The chemical properties of groundwater during cotton growing season in 2017–2019.

Properties pH HCO3− (g·L−1) Cl− (g·L−1) SO4 2− (g·L−1) Ca2+ (g·L−1) Mg2+ (g·L−1) K+ (g·L−1) Na+ (g·L−1)
Value 7.38 0.401 0.335 1.110 0.227 0.149 0.029 0.369

Daily air temperature and precipitation during the cotton growing seasons in 2017, 2018, and 2019.

 Experimental treatments and design

Field experiments utilized a drip irrigation system with film mulch. The study included six nitrogen levels (0 kg·ha−1, 150 kg·ha−1, 250 kg·ha−1, 300 kg·ha−1, 350 kg·ha−1, and 450 kg·ha−1) and two types of water (non-ionized brackish water and ionized brackish water). N fertilizers used in the experiments were urea (N ≥ 46%). This resulted in the NIF0, NIF1, NIF2, NIF3, NIF4, and NIF5 and IF0, IF1, IF2, IF3, IF4, and IF5 treatments, respectively. The 12 treatments were replicated four times in a randomized block design. Figure 2 reports the irrigation and fertilizer application schedule for the 2017, 2018, and 2019 cotton seasons in Xinjiang.

Schedule of irrigation and fertilizer during the cotton growing seasons in 2017 (A), 2018 (B), and 2019 (C).

Groundwater was employed as the non-ionized brackish water, while the ionized brackish water was taken as groundwater treated via the irrigation system ( Figure 3 ). Some properties such as electrical conductivity, pH, total dissolved solids, and surface tension of non-ionized brackish water and ionized brackish water were measured and are listed in Table 3 .

Drip irrigation system arrangement.

Properties of non-ionized brackish water and ionized brackish water.

Treatment Properties
Surface tension EC pH TDS
Non-ionized brackish water 72.0 2.95 7.38 2.2
Ionized brackish water 65.8 2.93 7.27 2.2
Sig p< 0.01 p > 0.01 p > 0.01 p > 0.01

EC is electrical conductivity; TDS is total dissolved solids; Sig is two-tailed p-value.

Irrigation was performed using a 16-mm-diameter drip line. The average emitter spacing was 30 cm. Water meters and ball valves were installed to control the amount of water applied to each plot. Each field plot was 5.6 m wide and 10 m long. The discharge rate for each drip emitter was 2.0 L·h−1.

Urea (N ≥ 46%) and potassium dihydrogen phosphate (KH2PO4 ≥ 99.5%) fertilizers were applied 14 times during the cotton growth stage. Differential pressure barrels with a 15-L capacity were used for the fertilizers. One barrel was placed in each experimental plot.

The cotton (Gossypium hirsutum L. Xinluzhong) was sowed on 22 April 2017, 24 April 2018, and 28 April 2019. Cotton was planted following the cultivation mode of plastic film mulching and short rod dense planting ( Figure 3 ). The narrow–wide–narrow row configuration of the system was set up with measurements of 20 cm + 40 cm + 20 cm. Two driplines were installed for four rows under a 1.1-m-wide film.

 Measurements and calculations Soil water and salt

To determine the water content and salinity of the soil, samples were collected at intervals of 10 cm from 0 to 40 cm and at intervals of 20 cm from 40 cm to 100 cm. This was done using a 5-cm-diameter auger in the middle of wide, narrow, and bare strips. Samples were gathered during the final stages of cotton’s main growth, just prior to irrigation and harvest. After each sampling, the experimental error was minimized by refilling all auger holes with soil. To determine the gravimetric soil water content, the soil samples were weighed, subjected to drying in a fan-assisted oven at a temperature of 105˚C for a duration of 24 h, and then reweighed. The volumetric soil water content was calculated by multiplying the gravimetric soil water content with the average bulk density of the soil profile at a depth of 100 cm. At a temperature of 25°C, the electrical conductivity of a 1:5 soil water extract (EC1:5) was measured using a DDS-307 conductivity meter. By applying a linear relationship (SC = 3.946*EC1:5, R 2 = 0.995, n = 30), the soil salt content (SC, g·kg−1) can be derived from the value EC1:5 obtained from each soil sample. The narrow soil strip’s soil salt accumulation was determined by subtracting the salinity content at the beginning of the growth stage from the salinity content at the end. Positive soil salt accumulation indicates the accumulation of salt in the soil, which negative accumulation suggests desalination has taken place.

 Crop evapotranspiration

Crop evapotranspiration (water consumption) was determined via the water balance equation as follows (Zhou et al., 2019):

E T c = P + I + G + Δ W − R 0 − F

where ET c represents crop evapotranspiration (mm), P represents the precipitation during the growing period (mm), I represents irrigation, G represents groundwater recharge (mm), ΔW represents the change in soil water storage in the 0–100 cm soil layer from sowing to maturity (mm), R 0 represents surface runoff (mm), and F represents deep percolation (mm). Since the water level in the experimental area was below 7 m, G, R 0, and F were considered insignificant, leading to the insignificance of Equation 1. Equation 1 transforms into Equation 2:

ET c = ± Δ W + P + I

Weights were assigned as proportions of the strip widths at different locations, and ΔW was calculated as Equation 3:

Δ W = 1000 ( 3 14 Δ θ b a r e + 1 2 Δ θ n a r r o w + 2 7 Δ θ w i d e )

where Δθbare, Δθnarrow, and Δθwide are the difference in volumetric soil water content for the bare, narrow, and wide soil strips between late seedling stage and harvest in 100 cm soil profile (cm3·cm−3), respectively.

 Leaf area index

During the seedling, bud, flowering, boll development, and boll-opening stages, four plants were chosen at random from every plot. To ascertain the leaf area, the dimensions of every leaf on the plants were measured using a tape measure (Jha et al., 2019). The calculation of the plant’s leaf area was performed in the following manner (Equation 4):

LA = ∑ i n A i ∗ B i ∗ 0.703

where LA represents the leaf area per plant (cm2), A i (cm) and B i (cm) represent the length and width of a leaf of cotton, n represents the number of leaves per plant of cotton, and 0.703 is the cotton leaf area correction factor (Wei et al., 2022).

The leaf area index (LAI) was then obtained as follows (Equation 5) (Wang et al., 2018; Wang X. et al., 2020):

LAI=LA T /S O

where LAT represents the total area of the cotton leaves (cm2) and SO represents the occupied land area (cm2).

 Shoot dry matter

At the seedling stage, bud stage, flowering phase, boll development stage, and boll-opening stage, four plants were randomly selected from each plot. The leaves, stem, squares, flowers, and bolls were placed into an oven at 105°C for 30 min and then dried at 75°C to constant weight and weighed. The average weight of four plants multiplied by the planting density per hectare represented the dry matter accumulation per hectare.

 Relative chlorophyll content (SPAD value)

The measurement of SPAD was conducted using a chlorophyll meter called SPAD-502 Plus. This was done on a functional cotton leaf that was young and fully expanded. The leaf chosen was the fourth one below the main stem terminal before the plant was topped, and the second leaf from the top after topping. In every plot, 15 leaves were chosen at random and two measurements were taken on each leaf, one on either side of the midrib.

 Cotton yield

The measurement of cotton production was conducted when 90% of the cotton bolls had opened. In each of the experimental plots, three sampling grids measuring 2.33 m × 2 m were established. Along these grids, a total of bolls were collected and their weights were measured (Wang et al., 2020).

 Water use efficiency

WUE (Equation 6) was calculated as follows (Gang et al., 2019):

WUE=Y/ET c

where Y represents the cotton yield (kg ha−1) and ET c represents the crop evapotranspiration (mm).

 Nitrogen use efficiency

Partial productivity of nitrogen (NPFP) (Equation 7) was calculated by Dai et al. (2023).

N P F P = Y / F t

where Y is the cotton yield (kg ha−1) and F t is the amount of fertilizer N applied (kg ha−1).

Agronomic nitrogen use efficiency (aNUE) (Equation 8) is defined as the increase in cotton yield per unit of fertilizer N applied (Zhang et al., 2012):

aNUE = ( Y − Y 0 ) / F t

where Y is the cotton yield (kg ha−1), Y 0 is the cotton yield of unfertilized treatment (kg ha−1), and F t is the amount of fertilizer N applied (kg ha−1).

Nitrogen apparent recovery efficiency (AREN) (Equation 9) is calculated by Xu et al. (2023).

A R E N = ( N U A − N U 0 ) / F N

Physiological nitrogen use efficiency (pNUE) (Equation 10) is defined as the increase in cotton yield per unit of increased N uptake as reported in Isfan (1990).

p N U E = Y − Y 0 N U A − N U 0

where F N represents the amount of fertilizer N applied (kg ha−1), NU A represents total N uptake of N-fertilizer plots (kg ha−1), and NU 0 represents total N uptake of zero N plots (kg ha−1).

The total nitrogen uptake (NU) (Equation 11) by crops is calculated by Li et al. (2021).

N U = N C × S D M

where NC represents plant nitrogen content (%) and SDM represents shoot dry matter weight (kg ha−1).

 Relationship between the cotton yield, shoot dry matter, WUE, and nitrogen application rates

A quadratic function was used to fit the relationship among the cotton yield, shoot dry matter, WUE, and nitrogen application rates. The quadratic equation (Equation 12) can be expressed as:

Y=aX 2 + bX+c

where Y represents the cotton yield (kg ha−1), X represents the nitrogen application rates (kg ha−1), and a, b, and c are coefficient.

 Data analysis

SPSS statistics 22 software was employed to perform analysis of variance (ANOVA). The average of four replicates was displayed for all indicators. The significant difference among all treatments was determined by least significant difference (LSD) at the p< 0.05 level. Origin 2021, Microsoft PowerPoint 2020, and Microsoft Excel 2020 were used to create figures and analyze data, respectively.

 Results Plant height

In 2017, 2018, and 2019, the results of this study exhibited significant impacts of nitrogen application rates and water types on the cotton plant height (p< 0.05, Figure 4 ). During 2017, 2018, and 2019, there was a trend of rapid growth followed by stabilization in cotton plant height, with maximum height being achieved at 81, 95, and 101 days after sowing, respectively. Plant height increased with nitrogen application rates in both the ionized and non-ionized treatment, with treatment IF5 maximizing plant height in 2017, 2018, and 2019. Ionized treatments exhibit a promotional effect on the maximum height of cotton plants in comparison to the non-ionized treatments.

Effects of nitrogen application rates and water type on the cotton plant height with the days after sowing (DAS) for 2017–2019 cotton growing seasons (A–F). Data are the mean value of the four replicates. Errors bars denote mean standard errors. Different letters above the bars indicate significant differences among treatments at p < 0.05.

 Leaf area index

LAI varied among water types and nitrogen application rates, but all showed an opening down unimodal curve as days after sowing ( Figure 5 ). A significant effect was exerted by water types and nitrogen application rates on LAI (p< 0.05). The LAI reached the peak at approximately 102, 95, and 106 days after sowing in 2017, 2018, and 2019, respectively. The peak value of LAI ranged from 3.3 to 4.6 in 2017, from 3.1 to 4.6 in 2018, and from 3.4 to 5.2 in 2019. Differences between nitrogen application rates and water type treatment of the LAI were small at the earlier stages of growth and then gradually increased. During the whole growth period, the LAI of ionized treatments was always higher than non-ionized treatments, by up to 5.2. Three-year results showed that the peak LAI of ionized treatments were 10.1%–15.0% higher than that of the non-ionized treatment.

Effect of water types and nitrogen application rates on the leaf area for 2017–2019 cotton growing seasons (A-F). Data are the mean value of the four replicates. Errors bars denote mean standard errors.

 Shoot dry matter

The shoot dry matter of cotton was different in water types and nitrogen application rates. The shoot dry matter of ionized treatments was higher than that of non-ionized treatments ( Figure 6 ). The shoot dry matter of IF4 was the highest in 3 years, and the highest was 27,870 kg·ha−1. The shoot dry matter of NIF1 was the lowest in 3 years, which was 12,003 kg·ha−1.

Effect of water types and nitrogen application rates on the shoot dry matter for 2017–2019 cotton growing seasons. Data are the mean value of the four replicates. Errors bars denote mean standard errors.

 Relative chlorophyll content (SPAD)

Relative chlorophyll content changes in the different nitrogen application rates and water types described a unimodal curve during the growth period ( Figure 7 ). The SPAD peak values of ionized treatments were generally higher than those of the non-ionized treatments. Furthermore, for the peak value of SPAD, IF5 was the highest and NIF1 was the lowest. Fertilizer had a significant positive effect on relative chlorophyll content. With the increase of nitrogen application rate, the SPAD increased. The results showed that the peak relative chlorophyll content of ionized treatments was 5.0%–13.4%, 5.5%–21.3%, and 11.9%–18.5% higher than that of the non-ionized treatment in 2017, 2018, and 2019, respectively.

Changes of SPAD with day after sowing for 2017–2019 cotton growing seasons (A–F). Data are the mean value of the four replicates. Errors bars denote mean standard errors.

 Cotton yield and water use efficiency

The nitrogen application rates and water types had a significant effect on cotton yield (p< 0.05) ( Table 4 ). During the years 2017, 2018, and 2019, the highest yield was observed when using non-ionized brackish water treatment NIF4, with a nitrogen application rate of 350 kg·ha−1, while the lowest yield was obtained with NIF0. The highest yield was observed when subjected to ionized brackish water treatment IF4, while the lowest yield was recorded under IF0. Fertilizer had a significant positive effect on cotton yield. With the increase of nitrogen application rate, the cotton yield firstly increased and then decreased. The results showed that the yield of ionized treatments was 11.3%–21.2%, 3.9%–13.4%, and 12.0%–29.2% higher than that of the non-ionized treatment in 2017, 2018, and 2019, respectively.

Effects of non-ionized brackish water and ionized brackish water nitrogen level on cotton yield and water and fertilizer use efficiency in 2017, 2018, and 2019.

Year Treatment Yield (kg·ha−1) P (mm) I (mm) ΔW (mm) ET c (mm) WUE (kg·ha−1·mm)
2017 NIF0 2,451.1f 64.4 487.5 – – –
NIF1 4,186.8e 64.4 487.5 7.9g 559.8g 7.5e
NIF2 5,543.6d 64.4 487.5 11.5f 563.4f 9.8d
NIF3 6,413.9c 64.4 487.5 19.1e 570.9e 11.2c
NIF4 6,991.9bc 64.4 487.5 28.6bc 580.5bc 12.0bc
NIF5 6,496.6c 64.4 487.5 27.0bc 578.9bc 11.2c
IF0 2,851.1f 64.4 487.5 – – –
IF1 4,657.9e 64.4 487.5 18.2e 570.1e 8.2e
IF2 6,468.6c 64.4 487.5 23.3d 575.2d 11.2c
IF3 7,771.4a 64.4 487.5 29.2b 581.1b 13.4a
IF4 7,820.3a 64.4 487.5 35.4a 587.3a 13.3a
IF5 7,406.7ab 64.4 487.5 26.3c 578.2c 12.8ab
2018 NIF0 2,312.5f 64.4 487.5 – – –
NIF1 3,854.9e 49.8 487.5 23.6g 560.9g 6.9e
NIF2 5,332.7d 49.8 487.5 31.9f 569.2f 9.4d
NIF3 6,169.4bc 49.8 487.5 37.1e 574.4e 10.7bc
NIF4 6,827.1a 49.8 487.5 44.0c 581.3c 11.7a
NIF5 6,338.8b 49.8 487.5 41.6cd 578.9cd 10.9b
IF0 2,562.5f 64.4 487.5 – – –
IF1 4,125.6e 49.8 487.5 32.4f 569.7f 7.2e
IF2 5,845.0c 49.8 487.5 39.8de 577.1de 10.1c
IF3 6,997.9a 49.8 487.5 47.6b 584.9b 12.0a
IF4 7,094.3a 49.8 487.5 56.4a 593.7a 11.9a
IF5 6,955.9a 49.8 487.5 50.6b 587.9b 11.9a
2019 NIF0 2,376.0f 64.4 487.5 – – –
NIF1 4,124.1e 19 487.5 42.3f 548.8f 7.5e
NIF2 5,720.2d 19 487.5 48.9e 555.4e 10.3cd
NIF3 6,464.6c 19 487.5 57.8d 564.3d 11.5bc
NIF4 7,080.5b 19 487.5 62.9c 569.4c 12.4b
NIF5 6,480.5bc 19 487.5 60.3cd 566.8cd 11.4bc
IF0 2,783.5f 64.4 487.5 – – –
IF1 4,619.8e 19 487.5 51.2e 557.7e 8.3de
IF2 7,043.6bc 19 487.5 63.1c 569.6c 12.4bc
IF3 8,352.5a 19 487.5 70.9b 577.4b 14.5a
IF4 8,408.2a 19 487.5 76.6a 583.1a 14.4a
IF5 7,903.7a 19 487.5 71.9b 578.4b 13.7a

P is precipitation; I is irrigation; ΔW is change in the soil water storage; ET c is crop evapotranspiration; WUE is water use efficiency. Data are mean of the four replicates. The different letters indicate significant differences between treatments at the p < 0.05 level according to the LSD test; the same letters are not significantly different at p > 0.05 level according to the LSD test.

Nitrogen application rates and water types had a significant effect on WUE (p< 0.05) ( Table 4 ). IF3 had the highest WUE, while NIF1 had the lowest. The average values over 3 years were 13.3 kg·ha−1·mm−1 for IF3 and 7.3 kg·ha−1·mm−1 for NIF1. The WUE of ionized treatments was 9.3%–19.6%, 1.7%–12.1%, and 10.7%–26.1% higher than that of the non-ionized treatment in 2017, 2018, and 2019, respectively.

 Relationship between seed cotton yield, shoot dry matter, and water use efficiency and nitrogen application rate

The relationship between seed cotton yield, shoot dry matter, WUE, and nitrogen application rate follows a quadratic curve ( Figure 8 ).

Relationship among nitrogen application rates and seed cotton yield (A, B), shoot dry matter (C, D), and water use efficiency (WUE) (E, F).

 Soil water content

The changes of the volumetric water content of the soil under ionized and non-ionized treatments are shown in Figure 9 . Fertilizer had a significant effect on soil water content. With the increase of nitrogen application rate, the soil water content decreased. The soil with ionized brackish water irrigation decreased the soil water content compared to the soil with non-ionized brackish water irrigation. The average soil water content of ionized treatments was 10.5%, 17.4%, and 13.6% lower than that of non-ionized treatments in 2017, 2018, and 2019, respectively.

Dynamics of soil volumetric water content in 0–40 cm from the seedling stage to maturity stage of cotton under non-ionized brackish water (A, C, E) and ionized brackish water (B, D, F) and nitrogen amounts in 2017, 2018, and 2019. Data are the mean value of the four replicates. Errors bars denote mean standard errors.

 Soil salinity

To avoid the influences of initial soil salt content and better evaluate the effects of ionized treatments and nitrogen application rates on soil salinity, the salt accumulation from late seedling stage to harvest was analyzed ( Table 5 ). Ionized treatments and nitrogen application rate affected soil salt accumulation (p< 0.05). Cotton roots were mainly located with the top 0–40 cm of soil under film-mulched drip irrigation. Therefore, the top 0–40 cm soil layer was considered as the main root zone of cotton in the following analysis. The soil salt accumulation of ionized treatments was 59.6%, 65.3%, and 51.8% lower than that of non-ionized treatments in 2017, 2018, and 2019, respectively. The soil salt desalination of the 0–40 cm soil profile was maximized at IF4 in 2017, but no significant differences in soil salt desalination were observed between IF3 and IF4. The soil salt desalination of the 40 cm soil profile was maximized at IF3 in 2018 and 2019, but no significant differences soil salt desalination were observed between IF3 and IF4.

Soil salt accumulation for all treatments in a narrow strip in the 40-cm and 100-cm soil profile in 2017, 2018, and 2019.

Treatment Soil salinity accumulation/g·m−2
2017 2018 2019
0–40 cm 0–100 cm 0–40 cm 0–100 cm 0–40 cm 0–100 cm
NIF1 286.2a 235.5f 155.9a 241.9d 192.8a 211.3g
NIF2 130.6bcd 342.5e 91.8abc 369.3c 125.4b 375.3e
NIF3 −261.0f 404.1cde −236.8e 487.5b −142.3c 438.8d
NIF4 −176.5e 465.7abc −91.7d 496.3b −104.1c 462.1cd
NIF5 165.7bc 496.5a 146.4a 520.4b 114.3b 632.5a
IF1 188.7b 265.4f 110.6ab 323.4c 127.8b 269.6f
IF2 102.5cd 368.5de 76.4c 475.7b 69.9b 436.5d
IF3 −389.9g 420.5bcd −303.2f 587.0a −216.9d 518.3bc
IF4 −416.1g 486.3ab −277.3ef 593.6a −201.7d 548.7b
IF5 71.1d 512.3a 77.9bc 619.8a 74.8b 664.1a

Different letters within a column indicate significant differences among all treatments, p< 0.05. “-” represent soil salt desalination.

 Nitrogen use efficiency

In 3 years, nitrogen application rate and water type had significant effects on aNUE, nitrogen apparent recovery efficiency, nitrogen physiological use efficiency, and nitrogen partial factor productivity (p< 0.05) ( Table 6 ). For the aNUE, IF3 was the highest and NIF5 was the lowest, and the 3-year average values were 16.9 kg·kg−1 and 8.8 kg·kg−1, respectively. With the increase of the nitrogen application rate, the aNUE firstly increased and then decreased. Average aNUE in the ionized treatment was 13.5%, 26.0%, and 24.3% higher than that of the non-ionized treatment in 2017, 2018, and 2019, respectively. For the nitrogen apparent recovery efficiency, IF3 was the highest and NIF1 was the lowest. With the increase of nitrogen application rate, the aNUE firstly increased and then decreased. pNUE decreased with the increase of nitrogen application rate. In 2017, the highest NPFP for IF1 treatment was 31.1 kg·ha−1, followed by 27.5 kg·ha−1 in 2018 and 30.8 kg·ha−1 in 2019. NPFP decreased with the increase of nitrogen application rate. Average aNUE in the ionized treatment was 14.5%, 8.8%, and 21.4% higher than that of the non-ionized treatment in 2017, 2018, and 2019, respectively.

Effects of different treatments on agronomic nitrogen use efficiency, nitrogen apparent recovery efficiency, nitrogen physiological use efficiency, and nitrogen partial factor productivity.

Year Treatment Agronomic nitrogen use efficiency (aNUE)/(kg/kg) Nitrogen apparent recovery efficiency (AREN)/(kg/kg) Nitrogen physiological use efficiency (pNUE)/(kg/kg) Nitrogen partial factor productivity (NPFP)/(kg/kg)
2017 NIF1 11.6bcd 0.470e 25.40a 27.9b
NIF2 12.4bcd 0.563de 23.50ab 22.2c
NIF3 13.2abc 0.598cde 22.15ab 21.4c
NIF4 13.0abc 0.673cd 19.36ab 20.0c
NIF5 9.0d 0.503e 18.03ab 14.4d
IF1 12.0bcd 0.483e 26.41a 31.1a
IF2 14.5ab 0.750bc 20.00ab 25.9b
IF3 16.4a 0.903a 18.38ab 25.9b
IF4 14.2ab 0.883ab 16.30ab 22.3c
IF5 10.1cd 0.665cd 15.16b 16.5d
2018 NIF1 8.6de 0.363e 24.55ab 25.7b
NIF2 11.1c 0.495cd 22.55abc 21.3d
NIF3 12.0 bc 0.595c 20.26bcd 20.6de
NIF4 12.2bc 0.711b 17.25cd 19.5e
NIF5 8.4c 0.528cd 15.93d 14.1f
IF1 12.1bc 0.468d 27.75a 27.5a
IF2 14.1ab 0.719b 19.64bcd 23.4c
IF3 15.6a 0.945a 16.52d 23.3c
IF4 13.7ab 0.937a 15.03d 20.3de
IF5 10.4cd 0.704b 14.67d 15.6f
2019 NIF1 11.7cd 0.381g 34.12a 27.5b
NIF2 12.7c 0.518ef 25.98abc 22.2cd
NIF3 13.6bc 0.697bc 19.46bc 21.5d
NIF4 13.5c 0.767b 17.54bc 20.2d
NIF5 9.1d 0.577de 15.87c 14.4f
IF1 12.2c 0.453fg 28.53ab 30.8a
IF2 17.0a 0.609cde 28.30ab 28.2b
IF3 18.6a 0.941a 19.75bc 27.8b
IF4 16.1ab 0.927a 17.34bc 24.0c
IF5 11.4cd 0.682bcd 16.75c 17.6e

Data are presented as mean values (n = 4); values followed by different lowercase letters indicate significance at p < 0.05.

 Discussion Effects of nitrogen application rates and water type on the plant height, LAI, chlorophyll content, shoot dry matter, and yield

Ionized fresh water significantly improves the plant height, aboveground biomass, and SPAD value of winter wheat (Wang X. et al., 2020). Our results are consistent with previous studies, which also showed that cotton grown with ionized brackish water exhibited greater plant height, LAI, aboveground biomass, and SPAD value compared to cotton grown with non-ionized brackish water. Nitrogen application also has a significant impact on aboveground growth. Increasing nitrogen application led to an increase in plant height, leaf area, and aboveground biomass of crops (Zhang et al., 2021; Qi and Pan, 2022; Snider et al., 2021). Si et al. (2020) conducted experiments on winter wheat and found that increasing nitrogen application enhanced the LAI and aboveground biomass, but these indicators declined when nitrogen was applied excessively. Wang S. et al. (2021) observed that increasing nitrogen application resulted in larger LAI and canopy photosynthetic rate in cotton. This study indicates that nitrogen application has a significant impact on plant height, LAI, and aboveground biomass. Plant height increases with increasing nitrogen application, while the LAI and aboveground biomass initially increase and then decrease with increasing nitrogen application. If the nitrogen application rate surpasses 350 kg·ha−1, the LAI and aboveground biomass cease to rise or may even exhibit a decline, suggesting that an excessive amount of nitrogen application could hinder the cotton’s growth.

Nitrogen in the soil can preferably be absorbed and utilized by crops in an inorganic form, and the transformation of nitrogen is closely related to crop uptake. The presence of nitrogen is essential for the production of proteins, nucleic acids, chlorophyll, and other important compounds in crops, while also governing the growth and development processes of crops. Nitrogen is a major component of chloroplasts, enabling plants to carry out photosynthesis and normal metabolism. The nitrogen nutrition level of leaves directly affects their photosynthetic activity, and within a certain range, the photosynthetic rate increases with increasing nitrogen application (Sui et al., 2013; Iqbal et al., 2020). In this study, under both non-ionized brackish water and ionized brackish water, the maximum SPAD value increased with increasing nitrogen application. When nitrogen application was the same, the maximum SPAD value under ionized brackish water was 5.0% to 13.4%, 5.5% to 21.3%, and 11.9% to 18.5% higher than under non-ionized brackish water. The reason why ionized brackish water can increase the SPAD value is mainly because it improves the soil water, salt, and nutrient environment; promotes the nitrogen uptake of cotton; and enhances the functional efficiency of the photosynthetic system.

The appropriate application of nitrogen fertilizer helps improve carbon–nitrogen metabolism. Nitrogen is one of the essential elements required for plant growth and participates in various metabolic processes within plants. Nitrogen application increases the adequacy of nitrogen supply within cotton plants, making it easier for them to absorb and utilize nutrients from soil, thus promoting photosynthesis and respiration. Additionally, nitrogen application can stimulate protein synthesis within cotton, enhancing photosynthesis efficiency and growth rate, consequently increasing carbon content with cotton, optimizing the balance between carbon and nitrogen metabolism, promoting cotton boll development, and thereby increasing the number and weight of cotton bolls per unit area (Stamatiadis et al., 2016). Adequate nitrogen supply promotes increased branching and leaf area in cotton, leading to the accumulation of photosynthetic products and increased cotton boll filling rate and weight, thus boosting yield. However, excessive nitrogen supply can result in excessive cotton growth, increased flower shedding, and reduced cotton boll yield and quality. The impact of various forms of nitrogen (NH4 + or NO3 −) applied in fertilizer on crop growth can be influenced by other factors such as climate, soil type, rhizosphere pH, and plant species. Chen et al. (2016) found that within the range of 0–375 kg·ha−1 of nitrogen application, both the number of cotton bolls and individual boll weight increased with increasing nitrogen application. Nevertheless, in the nitrogen application range of 375–600 kg·ha−1, there was a decline in both the quantity of bolls and the weight of each boll as the nitrogen application increased. This study also yielded consistent results. The application of fertilizer resulted in an increase in cotton yield; however, excessive nitrogen application may result in decreased yields (Albornoz and Lietn, 2015; Wang Z. et al., 2021). Similar results were obtained in this study. Studies have shown that either insufficient or excessive nitrogen application can lead to decreased yield (Raphael et al., 2019; Hassanzadehdelouei et al., 2022). The results of this study demonstrate that amount of nitrogen application significantly affects cotton yield and quality. Low nitrogen application limits the accumulation of cotton biomass and plant nitrogen accumulation, thus hindering high yields. Excessive nitrogen application leads to nitrogen wastage and cannot sustain increased yields. Therefore, the appropriate nitrogen application promotes cotton nitrogen absorption, which is consistent with previous research (Ma et al., 2022). The highest production was achieved with a nitrogen application rate of 350 kg·ha−1 under both NIF and IF circumstances, and yields started to decrease above 350 kg·ha−1. When the nitrogen application rate was the same, the IF treatment increased cotton yield by 11.3% to 21.2%, 3.9% to 13.4%, and 12.0% to 29.2% in 2017, 2018, and 2019 years, respectively. Hou et al. (2021) studied the impact of nitrogen application on cotton yield and concluded that cotton yield was related to nitrogen application in a quadratic polynomial manner, with maximum cotton yield achieved at a nitrogen application rate of 350 kg·ha−1, and cotton yield had no significant difference between 350 kg·ha−1and 400 kg·ha−1.

 Effects of nitrogen application rates and water type on soil water and soil salinity

In the 0–40 cm soil layer, under non-ionized brackish irrigation for 2017, 2018, and 2019, the average soil volumetric water content from the budding stage to maturity was 9.5%, 17.4%, and 13.6% higher compared to ionized brackish water irrigation, respectively. Soil volumetric water content gradually decreased with increasing nitrogen application rates. This is because higher nitrogen application rates promote crop growth, resulting in greater aboveground biomass and LAI, which, in turn, requires more water consumption. This observation is consistent with the findings of Kumar et al. (2022). Appropriate nitrogen application rates can reduce salt stress (Ding et al., 2010), promote crop growth, enhance crop nitrogen absorption (Zhang et al., 2018), and mitigate environmental pollution issues caused by excessive nitrogen fertilizer (Liu et al., 2021). Excessive nitrogen fertilizer input leads to the release of a large number protons through nitrification, which further induces an increase in alkaline cations, accelerating soil salinization (Yang et al., 2018). This study found that with increasing nitrogen application rates, the cumulative soil salt content in the 0–40 cm soil layer exhibited a trend of initially decreasing and then increasing, with higher levels observed under NIF irrigation compared to IF; this is because nitrogen application reduces soil pH, increases the dissolution of Ca2+ in the soil, and minimizes salt damage by maximizing the competition between Ca2+ and Na+. Nitrate can balance excess chloride ions and also reduce chloride salinity in the root zone (Dong et al., 2022).

 Effects of nitrogen application rates and water type on water use efficiency and nitrogen use efficiency

The previous research demonstrated an increase in WUE with increasing nitrogen application, followed by a decrease, reaching its peak at a nitrogen application rate of 300 kg·ha−1 (Chen et al., 2010). Similar results were obtained in this study, showing quadratic relationships between seed cotton yield, aboveground biomass, and WUE with nitrogen application rate.

Increasing nitrogen fertilizer costs and global concern for greenhouse gas emission have resulted in growing interest in improving N use efficiency over the past 20 years (Guo et al., 2022). Understanding how N use efficiency changes with N fertilization rates will assist producers in N management decisions that affect both the profitability and N impact on environment (Rochester, 2011). When evaluating nitrogen fertilizer utilization efficiency, various indicators are typically considered, including aNUE, nitrogen apparent recovery efficiency, pNUE, and partial productivity of nitrogen. aNUE represents the increase in grain yield per unit of nitrogen fertilizer input. Specifically, the nitrogen apparent recovery efficiency refers to the increase in grain yield per unit of nitrogen fertilizer input compared to the accumulation of nitrogen in aboveground plant parts. This concept was introduced for the first time in grain production. Partial productivity of nitrogen combines multiple factors such as soil nutrient levels and fertilizer application rates, making it an important indicator for assessing NUE. NUE is primarily influenced by factors such as crop genotype, farming practices, planting density, fertilizer type, and timing of application. Therefore, optimizing field management practices and nitrogen fertilizer application is a crucial pathway to improving nitrogen fertilizer utilization. In this study, under non-ionized brackish water (NIF) and ionized brackish water (IF) irrigation, nitrogen agronomic use efficiency and apparent use efficiency both showed an initial increase followed by a decrease with increasing nitrogen application. Nitrogen physiological use efficiency and partial productivity of nitrogen decreased with increasing nitrogen application. Nitrogen apparent use efficiency is an indicator of nitrogen absorption potential, reaching its maximum at a nitrogen application rate of 350 kg·ha−1 under non-ionized brackish water irrigation and at 300 kg·ha−1 under ionized brackish water irrigation, promoting nutrient absorption by cotton. Conversely, under ionized brackish water irrigation, nitrogen application can be reduced to achieve quality and efficiency goals. Cotton growth could be hindered and nitrogen absorption and utilization efficiency may decrease due to a high nitrogen application rate of 450 kg·ha−1.

 Conclusion

In 2017, 2018, and 2019, the utilization of 300 kg·ha−1 nitrogen application rate during ionized brackish water irrigation consistently led to elevated cotton yield, along with the highest WUE and aNUE. This may be considered the optimal combination of ionized brackish water and nitrogen application pattern for drip-irrigated cotton production in the Xinjiang. More studies are required to determine the ionized brackish water and nitrogen application pattern of drip-irrigated cotton with various soil types.

 Data availability statement

The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding author.

 Author contributions

KW: Writing – original draft. QW: Supervision, Writing – review & editing. MD: Supervision, Writing – review & editing. SL: Investigation, Writing – review & editing. YG: Investigation, Writing – review & editing.

 Funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Natural Science Foundation of China (grant numbers 52339003, 41830754, and 51679190), the Major Science and Technology Projects of the XPCC (2021AA003-2), and the Major Science and Technology Projects of Autonomous Region (2020A01003-3).

 Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

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