The tribe Triticeae within the subfamily Pooideae includes 27 genera, and 501 diploid and polyploid species (Soreng et al., 2015). Triticeae comprises several major crop species such as barley (Hordeum vulgare L.), rye (Secale cereale L.) and wheat, including bread wheat (Triticum aestivum L. ssp. aestivum) and durum wheat (Triticum turgidum L. ssp. durum). Wheat and barley are the founder crops of the Neolithic Agricultural Revolution in the Fertile Crescent of the Middle East, and they continue to be major cereal crops of temperate regions globally to the present day. Not surprisingly, extensive efforts have been made to study Triticeae crops throughout the world. However, Triticeae crops lagged since crop studies have entered the molecular genetic era, mainly due to the large genome sizes of Triticeae crops. After the completion of rice (Oryza sativa) and maize (Zea mays) genome sequencing, it has been relatively routine to identify causal genes underlying agronomic traits (Liu and Yan, 2019).

The bread wheat genome is substantially larger than barley and has a complex evolutionary history. Like barley, bread wheat also originated in the Fertile Crescent. This hexaploid species contains three subgenomes (A, B, and D) obtained through two hybridization events. In the first round of hybridization, two diploid wild ancestors created wild emmer wheat (T. turgidum L. ssp. dicoccoides) (BBAA) (Haas et al., 2019). The second hybridization event occurred at least 8,800 years ago and involved a cross between free-threshing tetraploid wheat and the donor of the D genome, Aegilops tauschii Coss. (DD) (Salamini et al., 2002; Zhao et al., 2023). Furthermore, the D subgenome possibly resulted from an ancient hybridization event followed by gene loss (Marcussen et al., 2014). Following these duplication events, highly similar genes coexist among the B, A, and D subgenomes. The large Triticeae crop genomes have a high content of transposable elements, which impeded genome assembly. Furthermore, wheat is polyploid, further complicating genome sequencing and assembly.

The genome of Arabidopsis has assembled two decades ago, which heralds the beginning of plant genome sequencing (Sun et al., 2022a). A reference-level genome provides basic information for plant research. Importantly, well-assembled and annotated genomes are even more important in studies of crop domestication, natural variation, and breeding (Sun et al., 2022a). DNA sequencing started with Sanger sequencing in 1977. Shotgun sequencing emerged in 1982. With the advent of ‘next-generation’ DNA sequencing (NGS) in 2005, the number of genome assemblies increased vastly. The third-generation sequencing technology has been developed for nearly a decade (Shendure et al., 2017). With the development of these sequencing technologies, algorithms, and software for high throughput data analysis were also continuously developed and updated. All these technological advances had made significant contributions to complex genome assembling (Shendure et al., 2017) ( Figure 1 ). To continuously explore and update the huge genome of wheat and barley, International Wheat Genome Sequencing Consortium (IWGSC) and International Barley Genome Sequencing Consortium (IBGSC) were established. The research on functional genomics of wheat lagged far behind that of rice and maize, which restricted the development of gene cloning and molecular design breeding techniques for important agronomic traits. A high-quality reference genome sequence map is the infrastructure to achieve a breakthrough (Brenchley et al., 2012).

Barley is the trendsetter in Triticeae genome sequencing. Many technological breakthroughs for Triticeae sequencing are first demonstrated in barley ( Table 1 ). Barley (2n = 2x = 14, HH) is an important cereal crop with a genome size of 5.1 Gb and a repeat sequence ratio of up to 84%. The first effort was to develop a chromosome draft sequence based on flow-sorted chromosomes (Mayer et al., 2011). Following that, a whole-genome shotgun-based draft genome sequence was released for ‘Morex’, a hulled barley cultivar, and 26,159 high-confidence genes were annotated (Consortium TIBGS, 2012). By employing chromosome conformation capture (Hi-C), a highly contiguous chromosome-scale genome assembly was obtained, in which the scaffold N50 length was increased to 1.9 Mb from 1.4 kb in the previous draft genome, and 39,734 high-confidence genes were annotated (Mascher et al., 2017). Subsequently, Genebanks was established through the collection of barley data. Genebanks can provide insight into the global population structure of domesticated barley by analyzing genome-wide genotyping data for almost all barley germplasm (Milner et al., 2018).

The tool TRITEX (Illumina PE/MP, 10 × Genomics and Hi-C technology) was used to obtain a 4.65 Gb barley reference genome (Morex V2), with increased scaffold N50 up to 40.2 Mb, and 32,787 high confidence genes were annotated. The BUSCO evaluation index was increased to 97.8% from 92.5% in the Morex V1 version (Monat et al., 2019). Based on the TRITEX strategy, the Morex V3 barley reference genome with a size of 4.50 Gb was assembled by combining third-generation of sequencing techniques (PacBio long read length and cyclic consensus sequencing data; Nanopore long read length data). The scaffold N50 jumped to 118.9 Mb and 35,827 high-confidence genes were annotated (Mascher et al., 2021).

The Tibetan hulless barley (Hordeum vulgare L. var. nudum), also called “Qingke” in Chinese, is an important staple food for Tibetans (Zeng et al., 2015). The genome of Tibetan hulless barley “Lhasa Goumang” was assembled using the whole-genome shotgun method. The assembly obtained a 3.89-Gb size genome, accounting for 87% of the Tibetan hulless barley genome, and containing 39,197 protein-coding genes (Zeng et al., 2015). With the development of the latest third-generation sequencing technology, 4.84G genome assembly of another hulless barley cv. Zangqing320 has been completed (Dai et al., 2018).

As the ancestor of cultivated barley, wild barley (Hordeum spontaneum) has important research and application value in the evolution and domestication fields of barley, as well as genetic breeding. Therefore, it is of great significance to study the beneficial genes related to disease resistance and stress tolerance in wild barley. A 4.28 Gb high-quality draft assembly of wild barley accession AWCS276 from Iran was obtained with in-depth sequencing using the whole genome shotgun sequencing (150×), accounting for 93% of the entire genome. A total of 36,395 coding protein genes were predicted using genomic and transcriptome data (Liu et al., 2019). The most effective barley genome for genetic transformation obtained a 4.13-Gb genome by adding Hi-C data to increase the contiguity to full chromosome size (Schreiber et al., 2020). As a wild species of barley and wheat, Hordeum marinum Huds. (2n=2x=14, XaXa) had excellent characteristics such as salt tolerance and resistance to water-logging, and enriched genetic resources for crop improvement. A 3816 Mb reference level genome was assembled using third-generation, second-generation, and Hi-C data, with a contig N50 6.83 Mb (Kuang et al., 2022). The genome information and efficient gene editing system of sea barley had important value for the breeding improvement of Triticeae crops.

Following barley, the wheat genomes are unmasked step by step ( Table 1 ). The hexaploid bread wheat genome is by far the most conspicuous in the Triticeae tribe. The Chinese landrace Chinese Spring (CS) is chosen for sequencing as the reference first. Due to the extremely complex genome of wheat (2n = 6x = 42 chromosomes), the physical map of the 3B chromosome was first constructed using bacterial artificial chromosome (BAC) (Yu et al., 2008). Six years later, the first reference sequence of the 3B chromosome was finished, providing a proof-of-concept and template for sequencing the remaining chromosomes of wheat (Choulet et al., 2014). The first draft sequence used ~60,000 Illumina-based genic sequences to assemble and putatively assign to subgenomes using Triticum monococcum, Ae. tauschii, and Aegilops speltoides sequence data, a total of 17Gb sequences were assembled, and 94,000-96,000 genes were predicted (Brenchley et al., 2012). Following that, an ordered draft sequence was produced by shotgun sequencing of isolated chromosome arms (International Wheat Genome Sequencing Consortium, 2014). In this version, 124,201 gene loci were identified across homoeologous chromosomes and subgenomes. Subsequently, the assembly length of the wheat genome gradually increased (Chapman et al., 2015; Clavijo et al., 2017; Zimin et al., 2017a). Although high sequence similarity and structural conservation are generally retained between bread wheat subgenomes and corresponding diploid and tetraploid wheat relatives, dynamic and sporadic gene gain, loss, and duplication are also evident. By combining chromosome shotgun sequencing, pseudomolecules with high-density genetic (POPSEQ), BAC-based physical mapping, Hi-C, and Bionano optical mapping, a substantially updated full reference genome release, IWGSC RefSeq v1.0, was obtained. In this version, 97% (14.1 Gb) of the assembled 14.5-Gb genome (N50 of super scaffold = 22.8 Mb) was assigned and ordered to 21 chromosomes (Appels et al., 2018). The classical BAC-by-BAC strategy provides highly ordered, high-confidence, chromosome-level sequences. This approach has been applied to the long arm of chromosome 7D (Feng et al., 2020). In the more recently updated IWGSC RefSeq v2.1 version, using optical maps and PacBio long reads, scaffolds were anchored and corrected, increasing the length of pseudomolecules by 168 Mb (Zhu et al., 2021). The 14.26 Gb genome of the French bread wheat cultivar Renan was assembled by long reads produced on the Oxford Nanopore Technology PromethION device, with a scaffold N50 of 48 Mb (Aury et al., 2022). A 14.7-Gb assembled chromosome of South African bread wheat Kariega was obtained by combining high-fidelity long reading, optical mapping, and chromosome conformation capture (Athiyannan et al., 2022). The resulting assembly sequence has an order of magnitude more continuity than previous wheat sequences.

To decipher the complex wheat genomes, parallel efforts have been made to sequence and assemble diploid wheat ancestors, including Triticum urartu (AA) and Ae. tauschii (DD), tetraploid wild emmer and durum wheat (BBAA), and hexaploid bread wheat (BBAADD) ( Table 1 ). As for barley, these efforts have been accompanied by rapid progress in sequencing technologies and assembly algorithms. As such, reference genome assemblies have been substantially improved over just a few years.

For example, the first released draft of the T. urartu (AA) genome assembly employed an Illumina platform-based whole-genome shotgun sequencing strategy to obtain a total of 4.66 Gb genome sequence with a scaffold N50 length of 63.69 kb (Ling et al., 2013). By combining bacterial artificial chromosome (BAC)-by-BAC sequencing and PacBio long read-based whole-genome shotgun sequencing, the T. urartu genome assembly was subsequently improved, and a genome of 4.86 Gb in size with a scaffold N50 length of 344 kb was obtained (Ling et al., 2018). Similar progress has been made in Ae. tauschii (DD) genome sequencing (Jia et al., 2013; Luo et al., 2017; Zhao et al., 2017; Zimin et al., 2017b; Gaurav et al., 2021; Zhou et al., 2021), and emmer and durum wheat genome sequencing (Avni et al., 2017; Maccaferri et al., 2019). Through the optical maps, the genome sequence of wild emmer wheat was improved (Zhu et al., 2019).

The high-quality reference genome of Tibetan semi-wild wheat was obtained with 14.71 Gb in size and 118,078 annotated genes. It is the second reference genome of hexaploid wheat published in the world (Guo et al., 2020). Using PacBio circular consensus sequencing (CCS) with the HiFi approach, the most easily transformed wheat ‘Fielder’ genome was obtained, with an N50 greater than 20 Mb (Sato et al., 2021). By combining the sequencing strategy including DeNovoMAGIC-2, PacBio sequencing, and multiple mapping techniques, a high-quality genome of nitrogen-efficient wheat variety “Kenong 9204” was assembled, with a 14.77 Gb genome size, and the N50 was 21.87 Mb (Shi et al., 2022). AK58 was an elite Chinese common wheat cultivar and had a draft genome at the chromosome level (Jizeng Jia et al., 2021). The N50 of assembling was 715 Mb. A draft genome of Tibetan semi-wild wheat Zang1817 (Triticum aestivum ssp. tibetanum Shao) was assembled with an N50 of 37.62 Mb (Guo et al., 2020).

By employing these technical advances, the recently finished rye (Secale cereale L.) genome assemblies have reached a high standard ( Table 1 ). Rye is a diploid crop grown extensively as a grain, cover crop, and forage crop especially on marginal land. Rye is exceptionally stress tolerant and has been extensively introgressed into bread wheat to improve grain yield and biotic and abiotic stress tolerance (Crespo-Herrera et al., 2017; Ayalew et al., 2018). Through shotgun sequencing of the whole genome of the winter rye inbred line Lo7, a 2.8-Gb whole-genome draft sequence of rye was obtained (Bauer et al., 2017). This reference sequence represents almost the entire low-copy portion of the rye genome. Rye is an out-crossing crop, and there are more exchanges with wild gene pools. The diploid 7.9 Gb rye genome was much larger than the syntenic diploid barley and bread wheat subgenomes (Li et al., 2021; Rabanus-Wallace et al., 2021). The two released genome assemblies were of high quality, with a scaffold N50 size of 1.04 Gb for the Weining rye genome and 29.44 Mb for the Lo7 rye genome. These high-quality rye genome assemblies reveal a recent long terminal repeat-retrotransposon burst around 0.5 million years ago, explaining the genome size expansion. Notably, a series of genes related to disease resistance, as well as genes involved in seed storage, heading data, frost tolerance, and fertility restoration, have been identified in the rye chromosome arm 1RS. This chromosome arm has been of broad interest as it has translocated to bread wheat chromosome 1BL to form 1B1R translocation lines, which show strong resistance to stripe rust diseases and powdery mildew (Crespo-Herrera et al., 2017).

The release of reference genomes further enabled pan-genome analyses of Triticeae crops. Triticeae cereals are widely cultivated and selected in divergent environments throughout the world. Pan-genome analyses take advantage of the rich genetic resources and century-long germplasm collection efforts for these crops. The initial efforts relied on microarrays that can detect single nucleotide polymorphism (SNP) (Winfield et al., 2012). Following that, Illumina platform-based short reads resequencing had been employed, which did not rely on prior knowledge of the genome polymorphism (Montenegro et al., 2017). However, short reads resequencing cannot identify genome structure variations, and the application of long reads-based resequencing and de novo assembly has further expanded our understanding of genome diversity (Hao et al., 2020; Jayakodi et al., 2020; Walkowiak et al., 2020). In addition to the technical evolution, the sizes of the population being analyzed have also expanded fast ( Table 2 ).

Low-resolution pan-genome analyses uncovered the genetic adaption, domestication, and breeding history of Triticeae crops. Several recent pan-genome studies have been carried out on wheat (Montenegro et al., 2017; Walkowiak et al., 2020) and barley (Jayakodi et al., 2020). A year before the publication of Chinese Spring RefSeq genome v1.0, the first pan-genome containing 18 wheat species was constructed through highly in-depth next-generation sequencing. However, 12,150 genes with variants were not found in Chinese Spring. These genes are associated with stress and defense (Montenegro et al., 2017). Another study reported ten reference-quality pseudomolecule assemblies and five scaffold-level assemblies of hexaploid wheat. These high-quality assemblies facilitated the identification of extensive structural rearrangements and introgressions from wild relatives, which are related to grain yield and quality, resistance to stresses, and adaptation to diverse environments (Walkowiak et al., 2020). Improved long-read sequencing-based assembly held promise to identify large genome structure variations (Jayakodi et al., 2020; Walkowiak et al., 2020). In barley, a study using 20 representative accessions from 19,778 worldwide domesticated varieties not only resolved barley breeding history but also provided detailed views of two frequently identified inversions in current elite barley germplasm (Jayakodi et al., 2020).

Before the whole genome resequencing of wheat was conducted, 500,000 SNPs were obtained through targeted re-sequencing based upon NimbleGen array technology, of which 80% belonged to A, B, and D subgenome variations and 20% belonged to inter variability among varieties (Winfield et al., 2012). Through the whole exome capture and genotyping-by-sequencing approaches, the first haplotype map of wheat was generated, detailing the genetic differences among wheat sample lines worldwide (Jordan et al., 2015). Approximately 4500 years ago, wheat was introduced to China. SNP markers reveal the genetic diversity of wheat varieties in China (Akhunov et al., 2010; Wang et al., 2014). Meanwhile, using wheat Wheat660K gene chip and DArT-seq technology, the possible transmission routes of wheat after its introduction into China from abroad were analyzed (Zhou et al., 2018). The endemic Tibetan semi-wild wheat in China had been acclimated to Tibet varieties (Guo et al., 2020).

Although different landraces and cultivars were selected by different research teams, several key common conclusions can be drawn from bread wheat resequencing studies (Balfourier et al., 2019; Hao et al., 2020; Sansaloni et al., 2020; Zhou et al., 2020; Wang et al., 2021b; Zhao et al., 2023). It has long been proposed that after its formation in the Fertile Crescent during the Neolithic period, bread wheat germplasm spread via two routes along with ancient human migration. Genome sequence analyses provided solid evidence for this hypothesis, as population structure separated European and New World varieties from Asian varieties (Balfourier et al., 2019). After their dissemination, bread wheat populations adapt to local environments to become landraces. A notable finding was that Indian dwarf wheat (T. aestivum ssp. sphaerococcum), endemic to southern Pakistan and northwestern India, was likely a geographically isolated and low-diversity taxon (Zhou et al., 2020). A genome-wide genetic variation map of wheat (VMap 1.0) was constructed by resequencing 414 wheat materials (Zhou et al., 2020). Subsequently, this map was upgraded using whole genome sequencing data from 795 wheat materials (VMap 1.1) (Zhao et al., 2023). A large fraction of genome variations present in landraces had been lost in cultivars (Sansaloni et al., 2020). In the process of origin and domestication of polyploid wheat, interploidy introgression played an important role in restoring population genetic diversity (Wang et al., 2022). These works have revealed the original mechanism of domesticated wheat and the evolution law of long-term gradual domestication, accelerating the analysis of wheat evolution (Zhou et al., 2020; Wang et al., 2022; Zhao et al., 2023) ( Figure 2 ). Nevertheless, there have also been reshufflings over time, presumably related to modern breeding programs. For example, modern Chinese landraces possessed significant contributions from European varieties (Balfourier et al., 2019; Hao et al., 2020; Zhou et al., 2020). Through the resequencing of 145 landmark cultivars of wheat, the process of genome remodeling and optimization of wheat cultivars in China in the past 70 years has been revealed. It has been found that there were significant breeding selection asymmetries among the three subgenomes of wheat, providing a scientific basis and ideas for cultivating candidate backbone parents and assembly breeding (Hao et al., 2020). More variations from landraces had been incorporated into cultivars released after the 1960s (Balfourier et al., 2019). In the 1970s, wheat had the highest diversity of varieties in China (Wang et al., 2021b).

For the study of barley domestication trajectory, people sequenced the genome of the 6,000-year-old barley, and genetic analysis supports the current hypothesis that barley domestication occurred in the Upper Jordan Valley (Mascher et al., 2016; Russell et al., 2016). Targeted resequencing analysis showed that although the domesticated barley originated from the Fertile Crescent, the candidate domestication genes originated in its eastern and western regions (Pankin et al., 2018). Qingke was introduced to southern Tibet most likely via north Pakistan, India, and Nepal between 4,500 and 3,500 years ago. And Qingke maybe not be the origin of barley of the low genetic diversities (Zeng et al., 2018).

By whole genome sequencing and analyzing the 116 varieties containing wild, weedy, and cultivated rye germplasm resources, it was revealed that cultivated rye was domesticated from weedy rye (Sun et al., 2022b).

Alien introgressions have been identified by further including pan-genome analyses of tetraploid and diploid ears of wheat ( Figure 2 ; Table 2 ) (Zhou et al., 2020). Tetraploid wheat is the primary contributor to the high genetic diversity of the bread wheat A and B subgenomes (Cheng et al., 2019; He et al., 2019; Zhou et al., 2020). Among them, free-threshing domesticated emmers contribute more diversity than wild emmers or other domesticated emmers (Zhou et al., 2020). By contrast, diploid T. urartu had made very limited introgression contributions to bread wheat, which was distinct from previous assumptions (Zhou et al., 2020). Notably, gene flow from the tetraploid emmers significantly contributed to the genetic diversity of the A and B subgenomes in bread wheat. By contrast, the barrier to gene flow from the diploid T. urartu or Ae. tauschii to bread wheat was much higher, as they cannot cross easily with bread wheat. Gene flow from wild wheat (T. turgium subsp. dicoccoides) had a significant impact on single nucleotide polymorphisms (SNPs) throughout the wheat genome (He et al., 2019; Pont et al., 2019). After the publication of the wheat genome (Appels et al., 2018), the study by Cheng et al. was the first one to analyze genetic variations using resequencing data, and it was found that the genetic diversity of hexaploid wheat originated from intraspecific and interspecific hybridization (Cheng et al., 2019). Gene flow (gene migration) and chromosome rearrangement had important contributions to the formation of genetic diversity in common wheat, and the impact of gene flow on future breeding strategies had been evaluated (Przewieslik-Allen et al., 2021). The whole genome sequencing of 3000-year-old Egyptian emmer wheat revealed genetic similarities between ancient Egyptian wheat and modern Arab and Indian wheat, indicating the early eastward and southward spread of ancient Egyptian wheat (Scott et al., 2019). Using chips and genome resequencing, population genetic analysis was conducted on 168 wild emmer wheat. It was found that the wild emmer wheat utilized different adaptive strategies in the same domain environment, resulting in different evolutionary pathways (Wang et al., 2020b). Similarly, strong genetic selection occurred in wild barley populations for soil drought adaptation (Wang et al., 2018) and soil ecological differences (Bian et al., 2020).

Large genome size and genetic complexity significantly hamper gene function analyses in Triticeae crops through map-based cloning of key regulatory genes. Pan-genome analyses provided an efficient alternative strategy for identifying genes under selection during domestication and breeding (Haas et al., 2019). New inference models and methods have been formulated for identifying selective sweeps and localizing selection target sites throughout the genome. These methods aim at uncovering beneficial alleles from the vast majority of neutral variations. In barley, pan-genome studies had identified genomic regions putatively under selection, which harbored regulatory genes underlying several morphological traits (Milner et al., 2018; Jayakodi et al., 2020). Bristling awns is a beneficial trait for seed dispersal in wild barley but a nuisance for farmers when harvesting and for animals when chewing (Elbaum et al., 2007). Regulatory genes for awn length and awn roughness were found in GWAS peaks (Milner et al., 2018). Pan-genome analyses of hexaploid and diploid wheat populations had identified regions that harbor genes encoding putative regulators of agronomic traits (Walkowiak et al., 2020; Zhou et al., 2021). For example, analysis of the brittle rachis trait revealed convergent adaptation in different wheat populations (Zhou et al., 2020). Domesticated emmer wheat and einkorn wheat harbor independent mutations in orthologs of the barley Btr1 gene, which controls seed shattering.

When phenotypic data is available, a genome-wide association study (GWAS) is possible, in which genomes from a population are scanned for genetic variations that can be used to predict phenotypic traits (Liu and Yan, 2019). Using genomic tools to improve wheat is crucial for accelerating the improvement of trait inheritance (Russell et al., 2016; Juliana et al., 2019; Pont et al., 2019; Guo et al., 2020; Zeng et al., 2020; Joynson et al., 2021). It has been reported that 35 key traits had genomic predictability and demonstrated the potential of genomic selection for wheat. A large genome-wide association study is also conducted to identify several important loci for trait associations in 50 traits in South Asia, Africa, and the Americas (Juliana et al., 2019). In an exon sequencing analysis in wheat, 48 and 40 genomic loci were identified that they were significantly related to flowering stage and plant height variation, including known genes that control flowering stage (Ppd, VRN, FDL, WPCL) and Rht genes that affect plant height (Pont et al., 2019). Targeted sequencing data combined with genome-wide association analysis reveal genetic loci related to photosynthesis in wheat (Joynson et al., 2021). In Tibetan semi-wild wheat, important de-domestication sites were identified through association analysis, providing further evidence for de-domestication (Guo et al., 2020). Through exon sequencing analysis and a common garden experiment involving 267 landraces and wild accessions collected from diverse geographical locations, a study in barley shed light on environmental adaption, and identified allelic variation in barley associated with geographical adaptation, such as heading days related to seasonal temperature and plant height related to dryness variables (Russell et al., 2016). Using a metabolite-based genome-wide association study (mGWAS), the metabolic gene base for adapting to high levels of UV-B in highland barley was revealed, providing a reference for the improvement of highland barley varieties and the study of molecular genetic mechanisms of biological and abiotic stresses in other crops (Zeng et al., 2020). In addition, complete genomic information can facilitate the cloning of key genes. For example, after obtaining a reference-quality genome assembly from Aegilops sharonensis, the stem rust resistance gene Sr62 was cloned (Yu et al., 2022). The search for these key genes in development and resistance contributes to the development of Triticeae crop breeding.

The method of mutation accumulation has been used to prove that epigenetic modifications and environmental changes can affect the rate and location of mutations in the genome, and the importance of epigenetic modifications in mutation rate variation has been demonstrated (Habig et al., 2021) ( Table 3 ). Before the release of the IWGSC RefSeq v1.0 genome, the first epigenome map of wheat was drawn, and the results showed that the methylation of the three subgenomes and diploid wheat ancestors Aegilops tauschii is highly conserved (Gardiner et al., 2015). With the improvement of genomic information, more and more epigenetic studies have been conducted (Geng et al., 2019; Li et al., 2019; Concia et al., 2020; Yuan et al., 2020; Habig et al., 2021; Jia et al., 2021; Liu et al., 2021; Wang et al., 2021a). Genomic methylation can affect genome-specific gene expression and specific biological processes, such as affecting plant disease resistance (Geng et al., 2019; Li et al., 2019). Genome merging and segregation in tetraploid to hexaploid wheat cause dynamic and reversible DNA methylation, and these changes were related to changes in gene expression and transposon activity (Yuan et al., 2020). The histone modification of H3K27me3 is relatively stable in different ploidy wheat, but the peak levels of H3K27me2 increased with increasing ploidy levels (Liu et al., 2021). The subgenomic differentiation activity of homologous regulatory elements was related to the dynamic regulation of methylase complexes, demethylases, and H3K27me3 (Wang et al., 2021a).

The physical chromosome organization is vital in the polyploid genome (Concia et al., 2020; Jia et al., 2021; Yuan et al., 2022). Transposons play an important role in stabilizing subgenomic stability (Jia et al., 2021), which influences transcriptional regulation in wheat (Concia et al., 2020). Recent research has used OCEAN-C technology to map open chromatin interactions in different ploidy wheat, integrating chromatin accessibility, histone modification, and transcriptome to deeply analyze the long-distance interactions of open elements during the polyploidy process in hexaploidy wheat (Yuan et al., 2022).