Front. Plant Sci. Frontiers in Plant Science Front. Plant Sci. 1664-462X Frontiers Media S.A. 10.3389/fpls.2023.1220921 Plant Science Original Research Investigating Verticillium wilt occurrence in cotton and its risk management by the direct return of cotton plants infected with Verticillium dahliae to the field Zhang Guangjie 1 2 3 Meng Zhuo 1 2 3 Ge Hao 1 2 3 Yuan Jiali 1 2 3 Qiang Song 1 2 3 Jiang Ping’an 2 * Ma Deying 1 2 3 * 1 College of Agronomy, Xinjiang Agricultural University, Urumqi, China 2 Engineering Research Centre of Cotton, Ministry of Education, Xinjiang Agricultural University, Urumqi, China 3 Key Laboratory of the Pest Monitoring and Safety Control on Crop and Forest, Xinjiang Agricultural University, Urumqi, China

Edited by: Wànkuí Gong, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, China

Reviewed by: Tuyong Yi, Hunan Agricultural University, China; Zili Feng, Chinese Academy of Agricultural Sciences, China; Yuwen Yang, Jiangsu Academy of Agricultural Sciences (JAAS), China

*Correspondence: Deying Ma, mdyxnd@163.com; Ping’an Jiang, jiang863863@sina.com

03 11 2023 2023 14 1220921 11 05 2023 12 10 2023 Copyright © 2023 Zhang, Meng, Ge, Yuan, Qiang, Jiang and Ma 2023 Zhang, Meng, Ge, Yuan, Qiang, Jiang and Ma

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Verticillium wilt is one of the most crucial diseases caused by Verticillium dahliae that threatens the cotton industry. Statistical results showed that the return of cotton plants infected with V. dahliae to the field might be an essential cause of the continuous aggravation of cotton Verticillium wilt. The correlation among the cotton plants infected with V. dahliae returning to the field, the occurrence of Verticillium wilt, and the number of microsclerotia in rhizosphere soil need further investigation. A potted experiment was carried out to explore the effects of the direct return of cotton plants infected with Verticillium dahliae to the field on the subsequent growth and Verticillium wilt occurrence in cotton. As a risk response plan, we investigated the feasibility of returning dung-sand (i.e., insect excreta) to the field, the dung-sand was from the larvae of Protaetia brevitarsis (Coleoptera: Cetoniidea) that were fed with the V. dahliae–infected cotton plants. The results demonstrated that the return of the entire cotton plants to the field presented a promotional effect on the growth and development of cotton, whereas the return of a single root stubble or cotton stalks had an inhibitive effect. The return of cotton stalks and root stubble infected with V. dahliae increased the risk and degree of Verticillium wilt occurrence. The disease index of Verticillium wilt occurrence in cotton was positively correlated with the number of microsclerotia in the rhizosphere soil. The disease index increased by 20.00%, and the number of soil microsclerotia increased by 8.37 fold in the treatment of returning root stubble infected with V. dahliae to the field. No Verticillium wilt microsclerotia were detected in the feed prepared from cotton stalks and root stubble fermented for more than 5 days or in the transformed dung-sand. There was no risk of inoculation with Verticillium wilt microsclerotia when the dung-sand was returned to the field. The indirect return of cotton plants infected with V. dahliae to the field by microorganism–insect systems is worthy of further exploration plan of the green prevention and control for Verticillium wilt and the sustainable development of the cotton industry.

 stalks returning to field cotton Verticillium wilt growth and development biotransformation green prevention and control section-in-acceptance Sustainable and Intelligent Phytoprotection Introduction

Xinjiang Uygur Autonomous Region is the largest cotton (Gossypium hirsutum L.)–growing region in China, with more than 2.5 million hectares planted and more than 5 million tons of cotton produced (Announcement of the National Bureau of Statistics, 2022), accounting for about 20% of the world’s cotton production (Wang et al., 2022). Because of limited land and water resources in northwest China, cotton fields that have been continuously cropped for over 20 years are prevalent in the Xinjiang cotton region (Wang et al., 2012). Cotton plants are rich in organic matter and nutrients. The direct return of cotton plants to the field has been carried out in Xinjiang for more than 40 years. Under the premise of proper operation, returning cotton plants to the field can promote the growth and development and yield of cotton (Wang et al., 2014; Lu et al., 2022; Xi et al., 2022; Yan et al., 2022; Zhang et al., 2023a). For instance, treatments with straw and straw biochar significantly enhanced most cotton growth parameters, consequently increasing the seed cotton yield from the second to the fourth year (Ma et al., 2019). Straw retention coupled with mineral phosphorus fertilizer reduces phosphorus fertilizer input and improves cotton yield in coastal saline soils (Cao et al., 2021). Straw returning coupled with nitrogen fertilization increases canopy photosynthetic capacity, yield, and nitrogen use efficiency in cotton (Hu et al., 2021). On the contrary, there are also negative reports on the growth and yield of cotton (Soon and Lupwayi, 2012). Verticillium wilt is a worldwide soil-borne fungal disease caused by Verticillium dahliae (Hampton et al., 1990; Eldon and Hillocks, 1996; Wang et al., 2004). It has become one of the crucial diseases threatening the sustainable development of the cotton industry and has shown an increasing trend year after year (Wang et al., 2011; Zhu et al., 2017; Liu et al., 2019). Microsclerotia are the primary survival structures in V. dahliae in soil and the primary infection source of cotton Verticillium wilt. Because of their strong adaptability, microsclerotia can survive in the soil for more than 10 years and are widely distributed in soil (Wilhelm, 1955; Devay et al., 1974). Studies have shown that the number of microsclerotia in rhizosphere soil is closely related to the occurrence of Verticillium wilt in crops (Harris and Yang, 1996; Barbara et al., 2007; López-Escudero and Blanco-López, 2007). Statistical findings from previous studies on cotton Verticillium wilt conducted across diverse regions and years of continuous cropping have consistently demonstrated that the re-introduction of V. dahliae–infected cotton plants into the field in successive years constitutes a significant contributing factor to the progressive exacerbation of cotton Verticillium wilt (Pullman and Devay, 1982; Paplomatas, 1992; Gao and Guo, 2020). However, the effects of returning cotton plants infected with V. dahliae to the field on the occurrence of Verticillium wilt and the number of microsclerotia of cotton in the next or current years remain unclear (Liu et al., 2018a; Luo et al., 2018).

The most effective method for preventing and controlling Verticillium wilt is breeding for disease resistance, but the progress could be faster. No cotton varieties have high resistance to cotton Verticillium wilt, and the chemical control is poorly effective and causes environmental pollution (Zhu et al., 2017). Biological control is considered the most ideal for controlling cotton Verticillium wilt. For instance, many studies have proven the biological control effect against verticillium wilt to be above 50% in greenhouse experiments; some studies have gradually applied it to the field, but its effectiveness in the field is not yet satisfactory (Yuan, 2006; Zheng et al., 2011; Lang et al., 2012; Li et al., 2013; Li et al., 2014; Wei et al., 2019a; Zhang et al., 2021). It is worth noting that the cultivation system of cotton plants returning directly to the field is closely related to the occurrence of cotton Verticillium wilt (Gao and Guo, 2020; Zhang et al., 2023b). How to properly make good use of cotton plants in diseased fields and realize green prevention and control of cotton Verticillium wilt is a problem worthy of further study (Wei et al., 2019b; Wheeler et al., 2019; Wheeler et al., 2020; Liu et al., 2021a). As a risk response plan, we plan to evaluate the feasibility of returning dung-sand (insect excreta, where the more prominent grain is called dung-sand) to the field, and the dung-sand was from the larvae of Protaetia brevitarsis Lewis (Coleoptera: Cetoniidea) that were fed with the V. dahliae–infected cotton plants.

P. brevitarsis is an insect belonging to the genus Protaetia, family Cetoniidae, and order Coleoptera (Ma, 1995; Ji et al., 2011). The larvae that are saprovorous and can efficiently break down crop stalks (Liu and Zhang, 2015; Yang et al., 2015; Zhang et al., 2019), livestock and poultry manure (Guo, 2020; Xu et al., 2021; Yang et al., 2022), edible fungus chaff (Zhang, 2015; Lee et al., 2018; Sun, 2018; Wei et al., 2020), and other organic wastes that decomposed microorganisms have pretreated. In particular, the larvae have an outstanding transformation ability for cotton stalks and can yield high-value products (Zhang et al., 2022). The larvae have the potential for application in feedingization (Lee et al., 2020; Ham et al., 2021; Nikkhah et al., 2021). Larvae dung-sand can be applied as fertilizer (Zhang et al., 2020). Previous studies have shown that larvae dung-sand is rich in humic acids and nutrient elements (Li et al., 2019) and has just begun to be used as an organic fertilizer or cultivation substrate for horticultural crops (Liu et al., 2018b; Lai et al., 2019; Wu et al., 2019; Shi et al., 2021; Joung et al., 2022), and the digestive system of the larvae of P. brevitarsis can kill plant pathogenic microorganisms (Xuan et al., 2022). However, there have been no studies on the transformation ability of the larvae of P. brevitarsis on the cotton stalks (aboveground part of the cotton plants) and root stubble (aboveground stubble of 10 cm + the underground root) infected with V. dahliae. The effects of decomposed microorganism and insect systems on the number of Verticillium wilt microsclerotia and the risk of returning dung-sand, which was from the larvae of P. brevitarsis fed with cotton plants infected with V. dahliae to the field are not known.

In summary, in the context of the large-scale direct return of cotton plants infected with V. dahliae to the field and the increasingly severe occurrence of Verticillium wilt in Xinjiang, it is essential to study the effects of these phenomena on cotton growth and development; in addition, the occurrence of Verticillium wilt and the number of microsclerotia in rhizosphere soil is worthy of further discussion. The feasibility of returning cotton plants infected with V. dahliae after transformation by larvae dung-sand is also worthy of further investigation. This study can provide data and reference schemes for the recycling of cotton plants infected with V. dahliae and the green control of cotton Verticillium wilt.

 Materials and methods Test site

The test site was located in the Industrialization Research Base of Environmental Insect Transforming Organic Waste, Xinjiang Agricultural University, in Manas County (44°13′49″ N, 86°23′3″ E), Changji Prefecture, China.

 Experimental materials

Cotton plants and potted soil were obtained from cotton Verticillium wilt fields around the test site. Cotton Verticillium wilt inoculants (wheat grains overgrown with V. dahliae) were purchased from the Cotton Research Institute of the Chinese Academy of Agricultural Sciences (Anyang, China); the strain type was common Verticillium wilt species (sclerotium type and deciduous type; the effective number of viable V. dahliae was 1.0 × 108 g−1) in the cotton fields of Xinjiang region. The cotton variety was Xinluzao 67 (Xinjiang Yanshi Dehai Agricultural Science and Technology Co., Ltd., Tiemengguan, China). The decomposition inoculant was VT (VT-1000, main functional bacteria are Bacillus, actinomycetes, lactic acid bacteria, and molds; effective number of viable bacteria was 2.0 × 1010 g−1; Beijing VOTO Biotechnology Co., Ltd., Beijing, China). Autoclave (vertical pressure steam sterilizer, YXC.5CS.1, Shanghai Bosun Medical Biological Instrument Co., Ltd., Shanghai, China), an instrument to measure chlorophyll (SPAD-502plus, Konica Minolta, Tokyo, Japan), electronic balance (LT3002, Changshu Tianliang Instrument Co., Ltd., Changshu, China), and vernier caliper (Hugong, 1–150 mm; Shanghai, China) were owned by the base. Flower pots (plastic material; the bottom has six small holes with a diameter of 1 cm, the upper and lower diameters are 30 cm and 24 cm, respectively; and the height is 30 cm), cotton seeds, tape, and other materials were purchased from the Shihezi farmers market. The larvae of P. brevitarsis were reproduced in the base.

 Experimental methods Effects of the direct return of cotton stalks and root stubble infected with <italic>Verticillium dahliae</italic> to the field on cotton growth and development

In advance, cotton plants infected with V. dahliae were divided into two parts: cotton stalks (the aboveground part of the cotton plants) and root stubble (the aboveground stubble of 10 cm + the underground root) (when cotton plants were mechanically harvested, all cotton leaves and a portion of cotton branches detached from the cotton plants, allowing for the harvest of dry weight of both cotton stalks and root stubble in a 1:1 ratio). The cotton stalks and root stubble were chopped into smaller pieces measuring less than 2 cm in length. The cotton stalks, root stubble, and cotton plants (half cotton stalks and half root stubble) were returned to the field with weights of 300 g m−2, 300 g m−2, and 600 g m−2 (the total collected amount of cotton plants was 6000 kg hm−2). The design of the potted experiment is shown in Table 1 . Cotton stalks or root stubble infected with V. dahliae were inoculated with Verticillium wilt inoculants at a rate of 6‰ of dry weight, whereas diseased soil was inoculated at a rate of 45 g m−2 (part of the samples was reserved for the determination of Verticillium wilt microsclerotia). Then, half of the potting substrate and additives was sterilized in the autoclave (121°C, 20 min; to ensure that V. dahliae is completely inactivated). The dry weight of each treated potted substrate was 10 kg (up to 25-cm deep). During the test, cultured pots were buried in the experimental field (the experimental field had not been planted with cotton in recent years) while keeping the top edge of the pots 2 cm above the ground (the purpose of this arrangement was to facilitate temperature and humidity to remain stable and reduce environmental interference). Two-meter-wide corridors separated the planting plots belonging to the diseased and sterilized experiment. For each treatment, 12 repeats were set up with control (CK) in the middle of the first six treatments; the layout was from north to south with six columns with aisle spacing of 60 cm. The additives or Verticillium wilt inoculants were uniformly returned to the top 15 cm of the soil before sowing. Four cotton seeds were planted in each pot and watered after sowing. After seedling emergence, one strong seedling was retained, and uniform management practices such as watering (2 L of water every 7 days), weeding (during the cotton seedling and bud stage), and pest control (for aphids and leaf mites) were implemented for all pots. The plant height, stem diameter, leaf age, height of the first fruiting branch, the number of fruiting branches, and chlorophyll content [Soil and plant analyzer development (SPAD) value and inverted three leaves] were investigated in each treatment and control at 30, 50, and 70 days, respectively.

Experimental design to study the effects of returning cotton plants infected with V. dahliae to the field on the growth and development of cotton and the occurrence of Verticillium wilt.

Treatments Potting substrate Additives
1 Sterilized diseased field soil Cotton stalks infected with V. dahliae
2 Root stubble infected with V. dahliae
3 Cotton plants infected with V. dahliae
4 Sterilized cotton stalks
5 Sterilized root stubble
6 Sterilized cotton plants
CK1 None
7 Diseased field soilwith V. dahliae Cotton stalks infected with V. dahliae
8 Root stubble infected with V. dahliae
9 Cotton plants infected with V. dahliae
10 Sterilized cotton stalks
11 Sterilized root stubble
12 Sterilized cotton plants
CK2 None
Effects of directly returning cotton stalks and root stubble infected with <italic>V. dahliae</italic> to the field on the occurrence of cotton Verticillium wilt and the number of microsclerotia in the rhizosphere soil

After allowing potted cotton to grow for 40 days, the grade of cotton Verticillium wilt was investigated (according to the five-grade classification described in GBT22101.5-2009, China); grading was then performed every 10 days. The average disease index of each of the four potted cotton plants was considered as one replicate, which was repeated thrice to calculate the disease index of each treatment. The equation to do so was as follows:

D i s e a s e   i n d e x = [ Σ ( n u m b e r   o f   d i s e a s e d   p l a n t s   a t   a l l   l e v e l s × c o r r e p o n d i n g   d i s e a s e   g r a d e ) / t o t a l   n u m b e r   o f   p l a n t s   i n v e s t i g a t e d × h i g h e s t   d i s e a s e   g r a d e   ( 4 ) ] × 100

After 70 days of growing potted cotton, samples of the rhizosphere soil from treatments 1–3 and 7–12, CK1, and CK2 (collected from 10 cm~15 cm below the surface, four pots as one replicate, repeated thrice) were collected. The number of Verticillium wilt microsclerotia was determined in the experimental and initial samples {the detection method was adapted from that of Wei et al. (2015) and was based on molecular biological detection methods [Synergy Brands (SYBR) Green real-time quantitative polymerase chain reaction of wet-sieving samples (wet-sieving Quantitative real time polymerase chain reaction (qPCR))] performed at the detection institution at the Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, China}. The aim of this study was to investigate the effects of the direct return of cotton stalks and root stubble infected with V. dahliae to the field on the occurrence of cotton Verticillium wilt and the number of microsclerotia in rhizosphere soil.

 Transformation ability of insect–microorganism composite technology on the cotton stalks and root stubble with <italic>V. dahliae</italic> and risk assessment of this system for the spreading of cotton Verticillium wilt

Silage machinery was used to harvest cotton stalks from the severely diseased fields where Verticillium wilt occurred. The lifting machinery pulled out the corresponding root stubble. A guillotine grinder crushed cotton stalks and root stubble, and, then, 60 kg (dry weight) of each was sterilized by the autoclave (121°C, 20 min). To ensure the adequacy and uniformity of the number of microsclerotia present after crushing the two materials, 160 kg of the cotton stalks and root stubble, according to 6‰ of dry weight of materials, was added the cotton Verticillium wilt inoculants, followed by adjustment of the water content to 65% ± 5%. The resulting materials were cultured at room temperature for 5 days.

The four mixed materials finally obtained were 60 kg of sterilized cotton stalks, sterilized root stubble, cotton stalks with V. dahliae, and root stubble with V. dahliae. These were supplemented with 40 kg of cattle manure (the main feed was wheat stalks, and no microsclerotia of cotton Verticillium wilt was detected) and 1‰ VT. The two controls contained 100 kg of cotton stalks infected with V. dahliae and root stubble with V. dahliae. The fermentation of materials (duration of 25 days) and the transformation of the larvae of P. brevitarsis were carried out according to the methodology of Zhang et al. (2022) [each culture box (1 L) was filled with 280 g of fresh material (about 80 g dry weight), 10 larvae (the third instar and 15th day) of P. brevitarsis were put into the box. The transformation experiment was carried out for 15 days. Each treatment was repeated four times]. After the transformation, we determined the weight gain, feed intake, and the yield of larvae dung-sand. In addition, the feed utilization rate, dung-sand conversion rate, and mortality were calculated with the method of Liu (2012). The equation used for calculation was as follows (mass unit = mg):

F e e d   u t i l i z a t i o n   r a t e   ( F U R ) = ( t o t a l   f e e d   w e i g h t − r e m a i n i n g   f e e d   w e i g h t ) / t o t a l   f e e d   w e i g h t × 100 % D u n g - s a n d   c o n v e r s i o n   r a t e = D u n g - s a n d   w e i g h t / ( f e e d i n g   w e i g h t − d r y   l a r v a e   w e i g h t   g a i n ) × 100 % M o r t a l i t y = N u m b e r   o f   d e a d   l a r v a e / n u m b e r   o f   t e s t e s   l a r v a e × 100 %

To evaluate the risk of the spread of cotton Verticillium wilt by the insect–microorganism composite technology, samples were collected at 0, 5, 10, 15, 20, and 25 days during the fermentation process of the six kinds of materials, respectively. The number of cotton Verticillium wilt microsclerotia was determined in these materials and the dung-sand from P. brevitarsis larvae that had been fed the six kinds of materials after fermenting it for 25 days (the specific detection method was referred to in Section 2.3.3). This section focuses on evaluating the effects of the fermentation process and the application of the insect–microorganism composite technology.

 Data processing

SPSS 23.0 was used to perform one-way analysis of variance and the Tukey’s multiple comparisons analysis to quantify the differences among the treatments (P< 0.05). Microsoft Excel 2013 was used to record and organize data and draw tables; Sigma Plot 14 was used to draw graphs.

 Results Effects of the direct return of cotton stalks and root stubble infected with <italic>Verticillium dahliae</italic> to the field on cotton growth and development

After being allowed to grow for 30 days ( Supplementary Table S1 ), for the potted cotton in the sterilized diseased field soil, the group of cotton plants infected with V. dahliae showed the best in plant height, stem diameter, leaf age, and chlorophyll content, followed by the sterilized cotton plants group. The group with sterilized cotton stalks showed the worst performance in the treatments and the CK1 (cotton plants unreturned to the field). Potted cotton grown in the diseased field soil infected with V. dahliae also showed the same trend. However, the growth and development of cotton were better than those in the sterilized diseased field soil, especially as there were significant differences in the plant height and stem diameter indexes (P< 0.05). The preliminary results showed that the total amount of cotton plants that were returned to the field was beneficial for the growth of cotton.

Fifty days after the growth of potted cotton ( Supplementary Table S2 ), the growth and development level of potted cotton in diseased soil was superior to that in sterilized soil, and the plant height index in seven treatments reached a significant difference level (P< 0.05). There were no significant differences in the plant height, the number of fruit branches, leaf age, and chlorophyll content of potted cotton in the treatments of the two potting substrates, respectively (P > 0.05); in the first fruiting branch height index, the cotton plants infected with V. dahliae group were significantly higher than those in the sterilized cotton stalks group (P< 0.05). The results showed that whether or not the potting substrate and the cotton plants were sterilized had an important effect on the growth and development of cotton.

When potted cotton grew to 70 days ( Table 2 ), regardless of whether diseased or sterilized soil was used as the potting substrate or if cotton plants were directly returned to the field or not, there were no significant differences in the growth and development of cotton as compared with the CK (P > 0.05). The overall performance of the seven treatments was as follows: cotton plants infected with V. dahliae > sterilized cotton plants > CK > root stubble infected with V. dahliae > sterilized root stubble > cotton stalks infected with V. dahliae > sterilized cotton stalk. The results showed that the total return of cotton plants to the field promoted the growth and development of cotton to some extent. Whereas, the return of root stubble and cotton stalks to the field alone had a certain negative influence on the growth and development of cotton. The growth and development rate of potted cotton in sterilized soil was generally lower than that in corresponding treatments using diseased soil.

Effect of the direct return to the field of cotton stalks and root stubble infected with V. dahliae on the growth and development of cotton (70th day).

Treatments Plant height (cm) Stems diameter (mm) First fruiting branch height(cm) Number of fruiting branches Leaf age Chlorophyll content (SPAD)
Sterilized diseased field soil Diseased field soil infected with V. dahliae Sterilized diseased field soil Diseased field soil infected with V. dahliae Sterilized diseased field soil Diseased field soil infected with V. dahliae Sterilized diseased field soil Diseased field soil infected with V. dahliae Sterilized diseased field soil Diseased field soil infected with V. dahliae Sterilized diseased field soil Diseased field soil infected with V. dahliae
Sterilized cotton stalks 40.01 ± 5.17 a 73.92 ± 6.34 a * 12.01 ± 3.58 a 12.63 ± 1.08 a 17.89 ± 0.49 b 29.97 ± 1.48 a * 5.58 ± 0.67 a 6.00 ± 0.58 a 11.88 ± 0.74 a 14.29 ± 0.99 a 52.04 ± 1.65 ab 55.43 ± 0.44 a *
Sterilized root stubble 59.73 ± 5.27 a 73.71 ± 4.92 a 11.49 ± 0.94 a 12.72 ± 0.71 a 23.29 ± 0.75 ab 29.84 ± 0.86 a * 6.75 ± 0.72 a 6.29 ± 0.59 a 14.54 ± 0.6 a 14.13 ± 0.47 a 55.38 ± 1.18 a 55.53 ± 1.27 a
Sterilized cotton plants 60.00 ± 5.28 a 82.63 ± 5.15 a * 11.74 ± 1.15 a 13.85 ± 0.90 a 27.19 ± 1.02 a 31.78 ± 1.32 a * 5.5 ± 0.69 a 6.92 ± 0.63 a 13.5 ± 0.62 a 14.33 ± 0.75 a 52.37 ± 0.99 ab 55.14 ± 0.82 a
Cotton stalks infected with V. dahliae 45.81 ± 8.15 a 73.4 ± 6.72 a * 9.24 ± 1.59 a 12.45 ± 0.93 a 22.59 ± 1.43 a 31.93 ± 1.42 a * 4.27 ± 0.85 a 5.91 ± 0.67 a 12.05 ± 0.94 a 13.64 ± 0.74 a 49.08 ± 1.28 b 54.49 ± 1.19 a *
Root stubble infected with V. dahliae 45.16 ± 7.20 a 77.38 ± 5.33 a * 8.20 ± 1.37 a 13.23 ± 0.88 a * 22.02 ± 2.42 ab 33.92 ± 1.62 a * 4.25 ± 0.72 a 6.25 ± 0.48 a * 11.71 ± 0.72 a 14.42 ± 0.66 a * 51.76 ± 0.94 ab 54.9 ± 0.95 a *
Cotton plants infected with V. dahliae 57.2 ± 5.67 a 88.07 ± 7.96 a * 10.09 ± 1.29 a 15.42 ± 1.1 a * 28.68 ± 1.63 a 33.79 ± 1.99 a 5 ± 0.65 a 7.91 ± 0.77 a * 12.27 ± 0.63 a 14.95 ± 0.68 a * 54.66 ± 1.9 ab 61.05 ± 2.36 a
CK 54.60 ± 6.96 a 82.18 ± 4.35 a * 9.88 ± 1.17 a 13.5 ± 0.59 a * 19.46 ± 1.84 b 34.97 ± 4.00 a * 5.33 ± 0.75 a 7.13 ± 0.50 a 12.04 ± 0.72 a 14.21 ± 0.40 a * 54.84 ± 1.94 ab 55.68 ± 0.9 a

Data in the table are means ± standard errors; columns without the same letter are significantly different from each other (Tukey methods, significant level P< 0.05); and paired t-tests were performed for the seven treatments of sterilized and diseased field soils. Asterisks (*) indicate significant differences. The same is below.

 Effects of directly returning cotton stalks and root stubble infected with <italic>V. dahliae</italic> to the field on the occurrence of cotton Verticillium wilt and the number of microsclerotia in rhizosphere soil

According to Figure 1 and Table 3 , after 50 days of the potted experiment, when diseased field soil containing V. dahliae was used as the potting substrate, Verticillium wilt began to appear in cotton plants from all five treatments except for the sterilized cotton stalks group and control treatments, began to present Verticillium wilt. The disease index of the root stubble infected with V. dahliae group was the highest (22.92 ± 7.51), followed by cotton plants infected with V. dahliae group (20.14 ± 12.05). When potted cotton grew for 70 days, Verticillium wilt occurred in the seven treatments, and the disease index of the group with root stubble infected with V. dahliae group was the highest (50.00 ± 9.55), which increased by 20.00% as compared with the CK (41.67 ± 5.51). This was followed by the group with sterilized root stubble (45.83 ± 10.42), in which the disease index increased by 9.98% as compared with the CK. The disease index of the other four treatments was lower than that of CK, and the disease index of the sterilized cotton stalk treatment was the lowest. When sterilized soil was used as the potting substrate, Verticillium wilt occurred only in groups where both the cotton plants and root stubble were infected with V. dahliae. The disease index of the cotton plants infected with V. dahliae group (8.33 ± 8.33) was higher than that of the group where root stubble was infected with V. dahliae (2.08 ± 2.08), but the overall incidence remained low. Preliminary results indicated that the direct return of root stubble to the field had an aggravating effect on the occurrence of cotton Verticillium wilt in diseased fields, especially the direct return of root stubble infected with V. dahliae. The disease index of Verticillium wilt in potted cotton grown in diseased field soil was much higher than that for potted cotton grown in sterilized soil. The direct return of cotton plants infected with V. dahliae to the field aggravated the early onset of Verticillium wilt in potted cotton in diseased fields. The diseased soil had an essential effect on the occurrence of Verticillium wilt in cotton.

Effect of the direct return to the field of cotton stalks and root stubble infected with V. dahliae on the occurrence of cotton Verticillium wilt [D50 (70): the 50th (70th) day of the growing of cotton plants in diseased soil containing V. dahliae; S70: the 70th day of the growing of cotton plants in sterilized diseased field soil; at 50th day, no cotton Verticillium wilt occurred in all treatments].

Effect of the direct return of the cotton stalks and root stubble infected with V. dahliae to the diseased soil containing V. dahliae on the rate of increase or decrease in disease index of the cotton Verticillium wilt.

Treatments Disease indexof the D70 Rate of increase or decrease (%)
Sterilized cotton stalks 12.50 ± 7.22 b −70.00
Sterilized root stubble 45.83 ± 10.42 ab +9.98
Sterilized cotton plants 27.08 ± 9.08 ab −35.01
Cotton Stalks infected with V. dahliae 20.83 ± 20.83 ab −50.01
Root stubble infected with V. dahliae 50.00 ± 9.55 a +20.00
Cotton plants infected with V. dahliae 29.17 ± 4.17 ab −30.00
CK 41.67 ± 5.51 ab

As can be seen from Table 4 , the number of cotton Verticillium wilt microsclerotia in the diseased rhizosphere soil of potted cotton increased by varying degrees in each treatment as compared with the original soil sample. The number of microsclerotia in the group with root stubble infected with V. dahliae group (26.43 ± 5.51 Colony-Forming Units (CFU) g−1) was significantly higher than that in the CK (3.55 ± 1.21 CFU g−1)(P< 0.05). The cotton plants infected with V. dahliae group (14.80 ± 0.44 CFU g−1) and the group where cotton stalks were infected with V. dahliae (9.97 ± 3.41 CFU g−1) followed. The numbers of microsclerotia in the three treatments were 8.37, 4.25, and 2.54 times more than those in the original soil samples, respectively. The numbers of Verticillium wilt microsclerotia in the other three treatments were similar to those in the CK; they ranged from 3.26 CFU g−1 to 4.98 CFU g−1. In the seven treatments containing sterilized soil potting cotton, the microsclerotia of cotton Verticillium wilt were detected in the three treatments of additives with V. dahliae, and the number of cotton Verticillium microsclerotia was significantly higher in the group of cotton stalks infected with V. dahliae and the group of cotton plants with V. dahliae than the group of root stubble infected with V. dahliae (0.20 ± 0.20 CFU g−1) (P< 0.05). This could be attributed to the high amount of microsclerotia in the initial cotton stalk sample (11.38 ± 5.77 CFU g−1) and the low amount of microsclerotia in the initial root stubble sample (0.08 ± 0.03 CFU g−1), as well as the uneven distribution of microsclerotia in the rhizosphere soil. Overall, the direct return of cotton stalks and root stubble infected with V. dahliae increased both the risk and degree of Verticillium wilt occurrence. The disease index of potted cotton ( Table 3 ) showed a positive correlation with the number of microsclerotia. However, despite having a low number of microsclerotia in the rhizosphere soil, sterilized root stubble resulted in a high disease index. The specific reasons behind these observations needed further exploration.

Effect of the direct return cotton stalks and root stubble with V. dahliae to the field on the number of cotton Verticillium wilt microsclerotia in the cotton rhizosphere soil (DS70: the samples on the 70th day of the cotton rhizosphere soil in diseased soil containing V. dahliae; SS70: the samples of the 70th day of the cotton rhizosphere soil in sterilized diseased field soil).

Treatments Number of cotton Verticillium wilt microsclerotia (CFU g−1) Growth multiples
DS70 Cotton stalks infected with V. dahliae 9.97 ± 5.91 b +2.54
Root stubble infected with V. dahliae 26.43 ± 9.55 a +8.37
Cotton plants infected with V. dahliae 14.80 ± 0.76 ab +4.25
Sterilized cotton stalks 4.98 ± 1.63 b +0.77
Sterilized root stubble 3.26 ± 0.46 b +0.16
Sterilized cotton plants 3.67 ± 0.70 b +0.30
CK 3.55 ± 2.09 b +0.26
Soil samples during sowing 2.82 ± 0.42
SS70 Cotton stalks infected with V. dahliae 5.02 ± 1.10 a –
Root stubble infected with V. Dahliae 0.20 ± 0.20 b –
Cotton plants infected with V. dahliae 3.21 ± 0.58 a –
Initial cotton stalks sample 11.38 ± 5.77 –
Initial root stubble sample 0.08 ± 0.03 –
Transformation ability of insect–microorganism composite technology for the cotton stalks and root stubble infected with <italic>V. dahliae</italic> and risk assessment of this system to spread cotton Verticillium wilt Effects of microbial fermentation process on the pile temperature of the six materials

As can be seen from Figure 2 , during the fermentation process, the temperature of each material pile initially increased and then fluctuatingly decreased; the temperature of each treatment was maintained above 50°C for the first 10 days, with a maximum temperature close to 70°C. After 22 days of fermentation, the temperature of each material pile decreased, whereas the temperature of the four compound feed piles decreased slowly and was still above 45°C. The temperature of the cotton stalks and root stubble infected with V. dahliae groups dropped to ~40°C. To reduce unnecessary testing costs, we measured the number of cotton Verticillium wilt microsclerotia in the six kinds of materials fermented for 5 days first. No microsclerotia of cotton Verticillium wilt were detected after 5 days of fermentation in any of these materials, indicating that the microbial fermentation process could easily kill the microsclerotia and thus reduce the risk of spreading cotton Verticillium wilt caused by the indirect return of cotton stalk to the field.

Effect of fermentation duration on the pile temperature of the six materials investigated here.

 Effects of microbial fermentation on the number of Verticillium wilt microsclerotia in six kinds of materials

Table 5 presents the difference in the ability of the larvae of P. brevitarsis to transform six kinds of materials fermented for 25 days. Under suitable conditions, the larvae of P. brevitarsis had good transformation ability for all kinds of compound feeds, especially for the cotton stalks infected with V. dahliae. For every 1 unit of dry larvae gains, the larvae of P. brevitarsis could consume 27.47 times of this feed and produce 23.88 times of larvae dung-sand. The performance of root stubble infected with V. dahliae compound feed was the second; the ability to transform sterilized cotton stalks and sterilized root stubble compound feed ranked third. The ability of the larvae of P. brevitarsis to transform fermented pure cotton stalks and root stubble infected with V. dahliae was poor; among these, the root stubble infected with V. dahliae group was the worst, and the feed utilization rate was only 44.14% ± 0.75%. Cotton Verticillium wilt microsclerotia were not detected in larvae dung-sand formed from the six kinds of transformed materials. Therefore, there was no risk of spreading cotton Verticillium wilt when cotton stalks and root stubble compounds fermented for 25 days were converted into larvae dung-sand.

Differences in the transforming capacity of the larvae of the Protaetia brevitarsis for six materials investigated here.

Processingtreatments Food intake (g) Larvae weight gain (g) Dung-sand weight (g) Feed utilization rate (%) Dung-sand conversion rate (%) Mortality (%)
Sterilized cotton stalks compound feed 58.14 ± 0.38 a 1.95 ± 0.08 c 48.16 ± 0.66 b 72.67 ± 0.47 a 85.69 ± 0.78 a 2.00 ± 2.00 a
Sterilized root stubble compound feed 51.90 ± 0.42 b 2.08 ± 0.07 ab 40.07 ± 0.38 c 64.87 ± 0.52 b 80.42 ± 0.19 b 2.00 ± 2.00 a
Cotton stalks infected with V. dahliae compound feed 59.61 ± 0.58 a 2.17 ± 0.03 ab 51.81 ± 0.33 a 74.52 ± 0.72 a 90.21 ± 0.71 a 0.00 ± 0.00 a
Root stubble infected with V. dahliae compound feed 57.76 ± 0.59 a 2.25 ± 0.02 a 47.47 ± 0.37 b 72.21 ± 0.74 a 85.53 ± 1.00 a 0.00 ± 0.00 a
Cotton stalks infected with V. dahliae 40.03 ± 0.71 c 1.95 ± 0.09 c 32.59 ± 0.24 d 50.04 ± 0.88 c 85.68 ± 1.61 a 2.00 ± 2.00 a
Root stubble infected with V. dahliae 35.31 ± 0.60 d 1.89 ± 0.08 c 26.17 ± 0.20 e 44.14 ± 0.75 d 78.40 ± 1.62 b 2.00 ± 2.00 a
Discussion Effects of the direct return of cotton stalks and root stubble infected with <italic>Verticillium dahliae</italic> to the field on cotton growth and development

Potted experiments were conducted in this study to investigate the effects of the direct return of cotton stalks and root stubble with V. dahliae on the growth and development of cotton and the occurrence of Verticillium wilt. The findings demonstrated that the total return of cotton plants to the field had a certain positive influence on the growth and development of cotton, which was consistent with the previous studies that had reported that returning stalks to the field could act as fertilizer for the subsequent seasons of cotton cultivation (Wang et al., 2014; Lu et al., 2022; Yan et al., 2022; Zhang et al., 2023a). However, this study also found that the return of cotton stalks and root stubble infected with V. dahliae to the field alone may have a particularly negative impact on the growth and development of cotton. This could be attributed to several factors, including the short return time of cotton plants to the field, the insufficient decomposition of organic matter by microorganisms, the autotoxicity resulting from the decomposition of the returned cotton plants, and the limited nitrogen availability leading to competition and comprises the fertilizer effect of returned plants (Tesio et al., 2012; Li and Zhang, 2016; Qin et al., 2022). As for the promotion of cotton growth and development by the total return of cotton plants to the field, it should be further verified whether root stubble can contribute to the decomposition of cotton stalks and vice versa. The direct return of cotton stalks and root stubble infected with V. dahliae to the field could also increase the number of microsclerotia in rhizosphere soil, thereby increasing the risk of Verticillium wilt in cotton and its negative effects (Wang et al., 2011; Wang et al., 2012; Liu et al., 2019). Contrarily, the growth rate of potted cotton with sterilized soil as potting substrate was generally lower than that of diseased soil as potting substrate. The growth and development of cotton in the three treatments infected with V. dahliae were also better than those in the three treatments supplemented with sterilized materials, indicating that both the microorganisms carried by cotton plants and soil microorganisms had important effects on the growth and development of cotton. While sterilized soil or materials killed the pathogen of cotton Verticillium wilt, they also eliminated other microorganisms beneficial to the growth and development of cotton (Wang et al., 2006; Yang et al., 2012; Kumar and Verma, 2018).

 Effects of the direct return of cotton stalks and root stubble infected with <italic>V. dahliae</italic> to the field on the occurrence of cotton Verticillium wilt and the number of microsclerotia in rhizosphere soil

This study proved that the direct return of cotton stalks and root stubble that had been infected with V. dahliae increased both the risk and degree of Verticillium wilt occurrence in the ongoing year. The number of microsclerotia of cotton Verticillium in the rhizosphere soil of the affected field increased by varying degrees (0.16–8.37 times) as compared with the initial soil samples during the peak of Verticillium wilt occurrence. There was a positive correlation between the disease index of cotton Verticillium wilt occurrence and the number of microsclerotia in the rhizosphere soil. However, the incidence and degree of cotton Verticillium wilt were dictated by combinations of inoculation and the fertilizer effect of cotton stalks and root stubble infected with V. dahliae being returned to the field. Other factors involved here were the base number of Verticillium wilt microsclerotia in the rhizosphere soil and environmental factors. Therefore, the overall situation was somewhat complicated (Guo et al., 2012; Yu, 2012; Liu et al., 2021b). It should be noted that the return of root stubble to the field significantly aggravated the occurrence of cotton Verticillium wilt in diseased fields, especially the return of root stubble infected with V. dahliae had the most significant impact on the occurrence of cotton Verticillium wilt. In this case, the disease index increased by 20.00%, and the number of Verticillium wilt microsclerotia increased by 8.37 times, possibly due to the cotton roots being a central part of the pathogen infestation of Verticillium wilt. The return of root stubble to the field created favorable conditions (the microsclerotia are released into the soil as the root stubble decays) for the propagation and infection of Verticillium wilt, increased the number of Verticillium wilt microsclerotia in the rhizosphere soil, and increased the risk and disease index of Verticillium wilt in cotton affected fields. The relevant mechanisms must be studied further (Raaijmakers et al., 2010; Zhu et al., 2017).

 Transformation ability of insect–microorganism composite technology on the cotton stalks and root stubble infected with <italic>V. dahliae</italic> and the risk assessment of this system to spread cotton Verticillium wilt

The results of this study showed that the larvae of P. brevitarsis have an outstanding transformation ability to ferment cotton stalks and root stubble materials. Under the optimal conditions, for every 1 unit of dry larvae gain, the larvae of P. brevitarsis could consume 27.47 times of this feed and produce 23.88 times of larvae dung-sand; the results were consistent with Zhang et al. (2022). According to our observations, the collectible amount of cotton plants was 6,000 kg hm−2. As per conservative estimates, each hectare of cotton plants could produce 3,000 kg of larvae dung-sand and 120 kg of dry larvae. The utilization of cotton stalk and root stubble infected with V. dahliae mediated by insect–microorganism composite technology has much potential for field applications (Zhang, 2022). To investigate the potential risk of cotton Verticillium wilt occurrence resulting from converting cotton stalks and root stubble infected with V. dahliae into larvae dung-sand, the effects of the fermentation process on the number of microsclerotia of cotton Verticillium wilt in cotton materials were investigated. After 5 days of fermentation, no microsclerotia of cotton Verticillium wilt were detected in all six fermentation materials, indicating that the high temperature continuously generated during the fermentation process effectively killed the microsclerotia of cotton Verticillium wilt. These observations are consistent with the previous results that reported the removal of microsclerotia of cotton Verticillium wilt when treated at 55°C for 6 h (Shang et al., 2013). Therefore, after 5 days of fermentation, none of the six fermentation materials studied here posed a risk of spreading the microsclerotia of cotton Verticillium wilt upon return to the field. In addition, we measured the number of microsclerotia in the larvae dung-sand obtained from the six fermentation materials after fermentation for 25 days; no microsclerotia were detected. Therefore, there is no risk of transmitting cotton Verticillium wilt when converting cotton stalks and root stubble infected with V. dahliae into larvae dung-sand. Larvae dung-sand is rich in nutrients and contains beneficial microorganisms, making it practical for application in horticultural crops (Li et al., 2019; Shi et al., 2021). In addition, it is friendly to the environment (Zhang et al., 2020). It has the advantage of potentially promoting the growth and development of cotton while reducing the occurrence of cotton Verticillium wilt. Therefore, it is worthwhile to carry out further relevant experiments on the return of larvae dung-sand to the field after the biotransformation of cotton plants infected with V. dahliae (Joung et al., 2022; Zhang et al., 2022).

 Conclusions

The direct return of cotton stalk and root stubble infected with V. dahliae has no noticeable effect on the growth and development of cotton in the current year, which may aggravate the occurrence of Verticillium wilt and the accumulation of microsclerotia in rhizosphere soil. As a risk response plan, the indirect return of cotton plants infected with V. dahliae to the field by insect–microorganism composite technology is worthy of further exploration for green prevention and control of Verticillium wilt and the sustainable development of the cotton industry.

 Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

 Author contributions

Experimental design and optimization: DM and PJ. Methodology: GZ and SQ. Validation: HG and JY. Data curation: GZ and ZM. Writing—original draft preparation: GZ and ZM. Writing—review and editing: DM and GZ. Supervision: PJ. Project administration: DM, SQ, and PJ. All authors contributed to the article and approved the submitted version.

 Funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the Autonomous Region Key R&D Program of Xinjiang, China (Grant No. 2022B02046) and the Postgraduate Research Innovation Program of Xinjiang Agricultural University (XJAUGRI2021002).

 Acknowledgments

The authors are grateful to Dr. Feng Wei and Mrs. Lihong Zhao from the Cotton Research Institute of the Chinese Academy of Agricultural Sciences for their help in designing the experiment and detecting Verticillium wilt microsclerotia. We also thank the reviewers for helping us improve our original manuscript.

 Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

 Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2023.1220921/full#supplementary-material

References Announcement of the National Bureau of Statistics on cotton production in 2022[EB/OL] . Available at: http://www.stats.gov.cn/sj/zxfb/202302/t20230203_1901299.html. Barbara D. J. Ortega A. Garcia J. J. Armengol J. (2007). Inoculum density-disease development relationship in Verticillium wilt of artichoke caused by Verticillium dahliae . Plant Dis. 91, 1131–1136. doi: 10.1094/PDIS-91-9-1131 Cao N. Wang J.-W. Pang J.-Y. Hu W. Bai H. Zhou Z.-G. . (2021). Straw retention coupled with mineral phosphorus fertilizer for reducing phosphorus fertilizer input and improving cotton yield in coastal saline soils. Field Crops Res. 274, 108309. doi: 10.1016/j.fcr.2021.108309 Devay J. E. Forreste L. L. Garber R. H. Butterfield E. J. (1974). Characteristics and concentration of propagules of Verticillium dahliae in air-dried field soils in relation to prevalence of Verticillium wilt in cotton. Phytopathology 64, 22–29. doi: 10.1094/Phyto-64-22 Eldon S. Hillocks R. J. (1996). The effect of reduced phytoalexin production on the resistance of upland cotton (Gossypium hirsutum) to Verticillium and Fusarium wilts. Ann. Appl. Biol. 129, 217–225. doi: 10.1111/j.1744-7348.1996.tb05746.x Gao F. Guo H.-S. (2020). Defense and intervention of plants and soil-borne pathogenic fungi. Chin. J. Nat. 42, 499–504. doi: 10.3969/j.issn.0253-9608.2020.00.001 Guo Y. (2020). Investigation on Agricultural Organic Waste Resources and Exploration on Conversion Mode of Agricultural Organic Waste Resources by Environment-Friendly Insects in Yuncheng County (Tai 'an, China: Master's Thesis, Shandong Agricultural University). Guo T.-F. Ma Y.-P. Li G.-P. Ding R.-R. Cai X.-L. Wang G.-P. . (2012). Study on occurrence regularity and control technology of Verticillium wilt of cotton in Kuitun Reclamation area. Hubei. Agric. Sci. 51, 20–22. doi: 10.3969/j.issn.0439-8114.2012.05.020 Ham Y.-K. Kim S.-W. Song D.-H. Kim H.-W. Kim I.-S. (2021). Nutritional composition of white-spotted flower chafer (Protaetia brevitarsis) larvae produced from commercial insect farms in Chesapeake. Food Sci. Anim. Resour. 41, 416–427. doi: 10.5851/kosfa.2021.e7 Hampton R. E. Wullschleger S. D. Oosterhuis D. M. (1990). Impact of Verticillium wilt on net photosynthesis, respiration and photorespiration in field-grown cotton (Gossypium hirsutum L.). Physiol. Mol. Plant Pathol. 37, 271–280. doi: 10.1016/0885-5765(90)90076-A Harris D. C. Yang Y. R. (1996). The relationship between the amount of Verticillium dahliae in soil and the incidence of strawberry wilt as a basis for disease risk prediction. Plant Pathol. 45, 106–114. doi: 10.1046/j.1365-3059.1996.d01-96.x Hu W. Yu C.-R. Zhao W.-Q. Liu R.-X. Yang C.-Q. Zhou Z.-G. (2021). The effects of straw-returning and inorganic K fertilizer on the carbon–nitrogen balance and reproductive growth of cotton. J. Cotton. Res. 4, 1–11. doi: 10.1186/s42397-021-00106-3 Ji B.-Z. Li S.-W. Zhang K. (2011). Entomological Basis and Common Species Identification (Beijing, China: Science Press), 251–252. Joung K.-H. Kim J.-W. Lee S.-B. Jang D.-H. Yoo B.-M. Bea S.-M. . (2022). Effects of Protaetia brevitarsis larvae manure application on lettuce growth and soil chemical properties. Korean. J. Soil. Sci. Fert. 55, 80–85. doi: 10.7745/kjssf.2022.55.1.080 Kumar A. Verma J. P. (2018). The role of microbes to improve crop productivity and soil health. Ecological Wisdom Inspired Restoration Engineering (Singapore: Springer Singapore), 249–265. Lai D.-Q. Wang Q.-L. Wu Y. Shu C.-L. Zhang Y. Liu C.-Q. (2019). Effect of Protaetia brevitarsis Lewis larvae dung on development of pepper seedling stage under low temperature. North. Hortic. 8, 63–66. doi: 10.11937/bfyy.20182738 Lang J.-J. Hu J. Ran W. Xu Y.-C. Shen Q.-R. (2012). Control of cotton Verticillium wilt and fungal diversity of rhizosphere soils by bio-organic fertilizer. Biol. Fertil. Soils. 48, 191–203. doi: 10.1007/s00374-011-0617-6 Lee S. Choi Y.-S. Jo K. Kim T.-K. Yong H.-I. Jung S. (2020). Quality characteristics and protein digestibility of Protaetia brevitarsis larvae. J. Anim. Sci. 62, 741–752. doi: 10.5187/jast.2020.62.5.741 Lee S.-B. Kim J.-W. Bae S.-M. Hwang Y.-H. Lee H.-S. Lee B.-J. . (2018). Evaluation of spent mushroom substrates as food for white spotted flower chafer, Protaetia brevitarsis Seulensis (Coleoptera: Cetoniidae). Korean. J. Appl. Entomol. 57, 97–104. doi: 10.5656/KSAE.2018.04.0.011 Li Y. Tong F. Geng L. Yu S. Chu H. Liu F. . (2019). Protaetia brevitarsis larvae can efficiently convert herbaceous and ligneous plant residues to humic acids. Waste. Manage. 83, 79–82. doi: 10.1016/j.wasman.2018.11.010 Li Z.-F. Wang L.-F. Feng Z.-L. Zhao L.-H. Zhu H.-Q. (2014). Diversity of endophytic fungi from different Verticillium-wilt-resistance Gossypium hirsutum and evaluation of antifungal activity against Verticillium dahliae in vitro . J. Microbiol. Biotechnol. 24, 1149–1161. doi: 10.4014/jmb.1402.02035 Li Y.-B. Zhang Q. (2016). Effects of naturally and microbially decomposed cotton stalks on cotton seedling growth. Arch. Agron. Soil Sci. 62, 1264–1270. doi: 10.1080/03650340.2015.1135327 Li S.-Q. Zhang N. Zhang Z.-H. Luo J. Shen B. Zhang R.-F. . (2013). Antagonist Bacillus subtilis HJ5 controls Verticillium wilt of cotton by root colonization and biofilm formation. Biol. Fertil. Soils. 49, 295–303. doi: 10.1007/s00374-012-0718-x Liu Y.-S. (2012). Insect Production Science (Beijing: Higher Education Press, China), 178–180. Liu F.-S. Feng X.-J. Xi G.-C. Wu Y. Wang Q.-L. (2018a). The effects of Protaetia brevitarsis larva manure application on the growth of cherry radish. Hubei. Agric. Sci. 57, 44–46, 50. doi: 10.14088/j.cnki.issn0439-8114.2018.04.011 Liu F. Tian C.-J. Lu X.-J. Han W.-B. Zhang Y. Ye S.-H. (2019). Effect of cotton straw returning planting model on the growth and pest and disease occurrence at the cotton seedling stage along the Yangtze River in Anhui Province. China Cotton. 46, 20–22. doi: 10.11963/1000-632X.lfysh.20191114 Liu H.-Y. Wang W. Zhang R.-F. Wenchimu A. Yao J. (2021b). Quantitative characteristics of microsclerotia of Verticillium dahliae in cotton soil under different factors. Xinjiang. Agric. Sci. 58, 522–531. doi: 10.6048/j.issn.1001-4330.2021.03.015 Liu X.-X. Woodward J. E. Kelly B. Lewis K. L. Byrd S. A. Chen Y. (2021a). Effects of production practices on temporal disease progress of Verticillium wilt of cotton (Gossypium hirsutum L.) in the Texas High Plains, USA. Crop Prot. 140, 1–8. doi: 10.1016/j.cropro.2020.105429 Liu H.-Y. Yao J. Zhang R.-F. Wang W. Yu X. Wang Q. (2018b). Soil microbial diversity in cotton fields with different degrees of Verticillium wilt. Acta Ecol. Sin. 38, 1619–1629. doi: 10.5846/stxb201702060218 Liu Y.-S. Zhang D.-P. (2015). Study on the model of microcirculation farm and ranch on the corn straw transformed by the larval of Protaetia brevitarsis Lewis. J. Anhui. Agric. Sci. 43, 85–87. doi: 10.3969/j.issn.0517-6611.2015.31.034 López-Escudero F. J. Blanco-López M. A. (2007). Relationship between the inoculum density of Verticillium dahliae and the progress of Verticillium wilt of olive. Plant Dis. 91, 1372–1378. doi: 10.1094/PDIS-91-11-1372 Lu H.-Q. Tang W. Zhang D.-M. Luo Z. Kong X.-Q. Li Z.-H. . (2022). Effects of fertilizer reduction and stalks returning on soil fertility, nutrient absorption, utilization, and cotton yield. Cotton. Sci. 34, 137–150. doi: 10.11963/cs20200037 Luo S.-W. Liu L. Liu Z. Ren Y.-Z. Li G.-Y. (2018). Quantitative detection of Verticillium wilt microsclerotia in soil. Xinjiang. Land. Reclam. Sci. Technol. 41, 20–22. Ma W.-Z. (1995). Chinese Economic Entomology (Coleoptera, Cetoniidea) Vol. 46 (Beijing: China Science and Technology Press, China), 94–95. Ma L.-J. Kong F.-X. Wang Z. Luo Y. Lv X.-B. Zhou Z.-G. . (2019). Growth and yield of cotton as affected by different straw returning modes with an equivalent carbon input. Field Crops Res. 243, 107616. doi: 10.1016/j.fcr.2019.107616 Nikkhah A. Van H.-S. Jovanovic V. Jung H. Dewulf J. Cirkovic V.-T. . (2021). Life cycle assessment of edible insects (Protaetia brevitarsis Seulensis larvae) as a future protein and fat source. Sci. Rep. 11, 14030. doi: 10.1038/s41598-021-93284-8 Paplomatas E. J. (1992). Incidence of Verticillium wilt and yield losses of cotton cultivars (Gossypium hirsutum) based on soil inoculum density of Verticillium dahliae . Phytopathology 82, 1417–1420. doi: 10.1094/Phyto-82-1417 Pullman G. S. Devay J. E. (1982). Epidemiology of Verticillium wilt of cotton: A relationship between inoculum density and disease progression. Phytopathology 72, 549–554. doi: 10.1094/Phyto-72-549 Qin X.-X. Wang Y.-M. Wang Z.-H. Xie C.-J. Wang B. (2022). Effects of straw returning on soil nutrients and microbial diversity in cotton fields. Xinjiang. Agric. Sci. 59, 1236–1244. doi: 10.6048/j.issn.1001-4330.2022.05.024 Raaijmakers J. M. Paulitz T. C. Alabouvette C. Monne-Loccoz Y. (2010). The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant Soil 321, 341–361. doi: 10.1007/978-90-481-2857-0 Shang W.-J. Chen T. Bai Y.-W. Yang J.-R. Hu X.-P. (2013). Germination conditions and lethal temperature of microsclerotia of Verticillium dahliae . Mycosystema 32, 986–992. Shi C.-X. Zhang G.-J. Xu Y.-S. Lu S.-S. Ai-Shanjiang E. R. Ma D.-Y. (2021). Effect of mixed substrate of larvae dung-sand of Protaetia brevitarsis on seedling cultivation of cucumber and pepper. J. Xinjiang. Agric. Univ. 44, 443–447. Soon Y.-K. Lupwayi N.-Z. (2012). Straw management in a cold semi-arid region: Impact on soil quality and crop productivity. Field Crops Res. 139, 39–46. doi: 10.1016/j.fcr.2012.10.010 Sun C.-K. (2018). Study on the Recycling Mode of "Wheat Straw-Stropharia rugosoannulata-Protaetia brevitarsis" (Tai 'an, China: Master's Thesis, Shandong Agricultural University). Tesio F. Vidotto F. Ferrero A. (2012). Allelopathic persistence of Helianthus tuberosus L. residues in the soil. Sci. Hortic. 135, 98–105. doi: 10.1016/j.scienta.2011.12.008 Wang J.-Y. Cai Y. Gou J.-Y. Mao Y.-B. Xu Y.-H. Jiang W.-H. . (2004). Vdnep, an elicitor from Verticillium dahliae, induces cotton plant wilting. Appl. Environ. Microbiol. 70, 4989–4995. doi: 10.1128/aem.70.8.4989-4995.2004 Wang J. Diao Z.-M. Zhang J. Ma S.-F. (2006). Effects and development prospects of soil microorganisms in promoting plant growth. Qinghai. pratac. 15, 20–26. doi: 10.3969/j.issn.1008-1445.2006.04.006 Wang L. Feng H.-Z. Gong M.-F. Zhou L.-G. (2011). Effects of different tillage practices on Verticillium wilt of cotton under mulched drip irrigation. Trans. Chin. Soci. Agric. Engin. 27, 1–6. doi: 10.3969/j.issn.1002-6819.2011.05.006 Wang L. Feng H.-Z. Teng L.-P. Chen X.-F. Dan H.-X. Lu S.-Q. . (2012). Effects of long-term cotton plantations on Fusarium and Verticillium wilt diseases infection in China. Afr. J. Agric. Res. 7, 1562–1565. doi: 10.5897/AJAR11.1532 Wang S.-L. Li J.-P. Zhao H.-L. Yu Q.-L. Song X.-L. Sun X.-Z. (2014). Analysis on utilization status and potential of cotton stalks returning to field. J. Shandong. Agric. Univ. (Natural. Sci. Edition). 45, 310–315. doi: 10.3969/j.issn.1000-2324.2014.02.028 Wang Y.-C. Peng S.-B. Huang J.-L. Zhang Y.-L. Feng L. Zhao W.-Q. . (2022). Prospects for cotton self-sufficiency in China by closing yield gaps. Eur. J. Agron. 133, 126437. doi: 10.1016/j.eja.2021.126437 Wei F. Fan R. Dong H.-T. Shang W.-J. Xu X.-W. Zhu H.-Q. . (2015). Threshold microsclerotial inoculum for cotton Verticillium wilt determined through wet-sieving and real-time quantitative PCR. Phytopathology 105, 220–229. doi: 10.1094/PHYTO-05-14-0139-R Wei P.-P. Li Y.-M. Lai D.-Q. Geng L.-L. Liu C.-Q. Zhang J. . (2020). Protaetia brevitarsis larvae can feed on and convert spent mushroom substrate from Auricularia auricula and Lentinula Edodes cultivation. Waste. Manage. 114, 234–239. doi: 10.1016/j.wasman.2020.07.009 Wei F. Zhang Y. Shi Y.-Q. Feng H.-J. Zhao L.-H. Feng Z.-L. . (2019a). Evaluation of the biocontrol potential of endophytic fungus Fusarium solani CEF559 against Verticillium dahliae in cotton plant. BioMed. Res. Int. 2019, 3187943. doi: 10.1155/2019/3187943 Wei F. Zhao L.-H. Xu X.-M. Feng H.-J. Zhu H.-Q. (2019b). Cultivar-dependent variation of the cotton rhizosphere and endosphere microbiome under field conditions. Front. Plant Sci. 10. doi: 10.3389/fpls.2019.01659 Wheeler T. A. Bordovsky J. P. Keeling J. W. (2019). The effectiveness of crop rotation on management of Verticillium wilt over time. Crop Prot. 121, 157–162. doi: 10.1016/j.cropro.2019.03.021 Wheeler T. A. Bordovsky J. P. Keeling J. W. (2020). The effects of tillage system and irrigation on Verticillium wilt and cotton yield. Crop Prot. 137, 105305. doi: 10.1016/j.cropro.2020.105305 Wilhelm S. (1955). Longevity of Verticillium wilt fungus in the laboratory and field. Phytopathology 45, 180–181. Wu X. Hu C.-Y. Cai R.-J. Xu X.-Y. Wang J.-L. Wang X.-B. (2019). Influence of frass organic manure on tomato growth and quality. North. Hortic. 426, 66–70. doi: 10.15933/j.cnki.1004-3268.2019.11.010 Xi K.-P. Yang S.-L. Xi J.-L. Li Y.-S. Zhang J.-C. Wu X.-P. (2022). Effects of long-term cotton stalks combined with organic fertilizer on soil physicochemical properties and yield. Soil Fertiliz. Sci. Chin. 7, 82–90. doi: 10.11838/sfsc.1673-6257.21226 Xu T. Zhang G.-J. Yang L. Qiang S. Ma D.-Y. Liu Y.-S. (2021). Technology for breeding Protaetia brevitarsis Lewis indoors and outdoors. Chin. J. Appl. Entomol. 58, 1000–1006. doi: 10.7679/j.issn.2095-1353.2021.100 Xuan H.-N. Gao P.-W. Du B.-H. Geng L.-L. Wang K. Huang K. . (2022). Characterization of microorganisms from Protaetia brevitarsis larva frass. Microorganisms 10, 311. doi: 10.3390/microorganisms10020311 Yan X.-Y. Guo W.-J. Qin D.-L. Wang S.-L. Nie J.-J. Zhao N. . (2022). Effects of cotton stalks returning and deep pine on dry matter accumulation, nutrient absorption and cotton yield in Binhai saline-alkali land. Acta Agronomica. Sin. 48, 1235–1247. doi: 10.3724/SP.J.1006.2022.14064 Yang C. Liu Y.-S. Xu X.-Y. Zhao L. (2015). The study on the effect of Protaetia brevitaris Lewis larvae transforming the corn straw. J. Environ. Entomol. 37, 122–127. doi: 10.3969/j.issn.1674-0858.2015.01.19 Yang G.-L. Tong J. Zhang L. Ma K. Song N.-P. (2012). Effects of fumigation and sterilization on growth and soil microorganisms of continuous cropping potato. Res. Agric. Sci. 33, 36–40, 56. doi: 10.3969/j.issn.1673-0747.2012.01.007 Yang L. Zhang G.-J. Xu T. Zhang L.-J. Qiang S. Ma D.-Y. . (2022). Study on the conversion capacity of different agricultural organic wastes by the larvae of the Protaetia brevitarsis Lewis. Xinjiang. Agric. Sci. 42, 189–193. Yu Z.-Z. (2012). Study on Quantitative Detection Technology of Microsclerotium Molecules of Verticillium Wilt in Soil (Xi'an, China: Master's Thesis, Northwest A&F University). Yuan L.-H. (2006). Effect of organic amendments on Verticillium wilt of cotton. Crop Prot. 25, 1167–1173. doi: 10.1016/j.cropro.2006.02.014 Zhang Q. (2015). Study on the Biology of Protaetia brevitarsis (Lewis) Feeding on Oyster Mushroom Bran (Tai 'an, China: Master's Thesis, Shandong Agricultural University). Zhang G.-J. (2022). Effects of the Return to the Field of Cotton Stalks Transformed by Insect-Microorganism Composite Technology on the Growth and Development of Cotton and the Occurrence of Verticillium Wilt (Urumqi, China: Doctoral Thesis, Xinjiang Agricultural University). Zhang L. Su X.-Y. Meng H. Men Y.-Q. Liu C.-M. Yan X.-Y. . (2023a). Cotton stubble return and subsoiling alter soil microbial community, carbon and nitrogen in coastal saline cotton fields. Soil Tillage. Res. 226, 105585. doi: 10.1016/j.still.2022.105585 Zhang G.-J. Wang Q. Liu Y.-S. (2020). Biology under artificial condition and utilization potential of Protaetia brevitarsis (Coleoptera: Cetoniidae). J. Environ. Entomol. 42, 24–33. doi: 10.3969/j.issn.1674-0858.2020.02.2 Zhang G.-J. Wang Q. Liu Y.-S. Li Z.-A. (2019). Study on the transformation capability of four materials in different fermentation cycles fed by Protaetia brevitarsis (Coleoptera: Cetoniidae) larvae. J. Shandong. Agric. Univ. 50, 764–767, 804. doi: 10.3969/j.issn.1000-2324.2019.05.006 Zhang G.-J. Xu Y.-S. Zhang S. Xu A.-D. Meng Z. Ge H. . (2022). Transformation capability optimization and product application potential of Protaetia brevitarsis (Coleoptera: Cetoniidae) larvae on cotton stalks. Insects 13, 1083. doi: 10.3390/insects13121083 Zhang Y. Yang Y.-X. Lu X.-Y. Wang A.-Y. Xue C. Zhao M. . (2023b). The effects and interrelationships of intercropping on cotton Verticillium wilt and soil microbial communities. BMC Microbiol. 23, 41–55. doi: 10.1186/s12866-023-02780-6 Zhang Y. Zhu H.-Q. Ye Y.-H. Tang C.-M. (2021). Antifungal activity of Chaetoviridin A from Chaetomium globosum CEF-082 metabolites against Verticillium dahliae in cotton. Mol. Plant Microbe Interact. 34, 758–769. doi: 10.1094/MPMI-02-21-0032-R Zheng Y. Xue Q.-Y. Xu L.-L. Xu Q. Lu S. Gu C. . (2011). A screening strategy of fungal biocontrol agents towards Verticillium wilt of cotton. Biol. Control. 56, 209–216. doi: 10.1016/j.biocontrol.2010.11.010 Zhu H.-Q. Li Z.-F. Feng Z.-L. Feng H.-J. Wei F. Zhao L.-H. . (2017). Ten years review and prospect of cotton Verticillium wilt research in China. Cotton. Sci. 29, 37–51. doi: 10.11963/1002-7807.zhqzhq.20170825