ZFN was the first engineered nuclease that are widely employed to create DNA break resulting in the activation of DNA repair machinery (Petolino, 2015). ZFNs typically employed their “three fingers” to detect an 18 base pair target sequence in DNA. ZFNs are among the most powerful and flexible nucleases, with two domains: one for DNA binding and one for DNA cleavage. For DNA double-strand breakage, the type II restriction endonuclease FokI dimerizes to cleave the DNA strand (Smith et al., 2000). DSBs in DNA are repaired by two pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ), resulting in gene mutations and gene knock-in or knock-down (Maeder et al., 2008; Foley et al., 2009; Lee et al., 2010). Research has shown that ZFNs with a larger number of zinc fingers (4, 5, and 6 fingers) are more specific and efficient at their intended targets. ZFNs have been used to produce fertile transgenic soybean plants using NHEJ mediated targeted insertions of multigene donors into an endogenous genomic locus (FAD21a) of embryogenic cells (Bonawitz et al., 2019). So far, ZFNs have been used to modify the genetic makeup of many different plant species, including arabidopsis, tomato, rice, apple, tobacco, petunia, soybean, maize, and fig (Gaj et al., 2013; Cantos et al., 2014; Hilioti et al., 2014; Martinez-Fortun et al., 2017; Ran et al., 2017). The principal drawbacks of ZFNs include labor-intensive, complex and expensive construction of protein domains for each specific locus target in the genome, as well as imprecise cleavage in the target sequence of the genome owing to erroneous protein domain interaction.

TALENs, like ZFNs, are made up of transcriptional activator-like effector (TALE) repeats and the FokI restriction enzyme. Because each TALE repeat selectively targets a single nucleotide, TALE repeats may target more target sites than ZFNs, allowing for more versatile target design. Many plant species have successfully been altered their genomes using TALENs (Ran et al., 2017). TALENs mediated targeted mutagenesis of the Caffeic acid O-methyltransferase replaced with Caffeic acid O-methyltransferase (COMT) in sugarcane resulted in improved the cell wall composition for enhanced bioethanol production (Jung and Altpeter, 2016; Zaman et al., 2019). Similarly, multi-allelic mutagenesis of COMT mutants using TALENs resulted in the reduction of 19.7% lignin content and a decrease in syringyl to guaiacyl (S/G) ratio with improved saccharification efficiency. Such targeted alterations in the COMT gene increased sugar content in sugarcane without causing a loss of biomass (Kannan et al., 2018). By interrupting the genes for fatty acid desaturase (FAD) using TALENs, soybeans with high oleic acid and low linoleic acid contents were produced, suggesting that this technology may be utilised to alter the nutritional profiles of crops (Demorest et al., 2016). Similarly, the betaine aldehyde dehydrogenase (BADH2) gene has been disrupted using TALEN technology to make rice with fragrance (Shan et al., 2015). In order to create purple tomatoes with increased anthocyanin levels, a strong promoter was inserted upstream of the gene regulatinganthocyanin biosynthesis using TALENs (Čermák et al., 2015). These examples demonstrate the enormous potential of the TALEN as gene editing technology for enhancing commercially important agricultural traits. Likewise, ZFNs, this genome editing tool has a few drawbacks such as it is laborious, time consuming and have low mutation rate (Becker and Boch, 2021).

CRISPR-Cas based genome editing has sparked another revolution in targeted genome engineering. Unlike ZFNs and TALENs, CRISPR-Cas9 based genome editing is effective, economical, and effortless in generating DSBs in DNA. In addition, it facilitates the creation of any genomic target, multiplexing, simple prediction of off-target regions, and straightforward delivery. CRISPR-Cas9 system originated from Streptococcus pyogenes (Sp) is well conserved. Its discovery was a 20th-century breakthrough since it is a unique technology that is being explored by researchers from different fields such as bioinformatics, biotechnology, and microbiology (Miki et al., 2018). Due of its versatility, simplicity, efficacy, and wide range of applications, the CRISPR-Cas9 system is used in fundamental as well as applied research. CRISPR-Cas9 uses DNA–RNA interaction and requires a target-site-specific 18–20 bp oligonucleotide sgRNA (Single guide RNA) to edit genes. In case of SpCas9, designed gRNA binds to a 5′-NGG-3′ protospacer adjacent motif (PAM) sequence at the 3′ end of the target sequence Cas9 is often used to generate DSBs at the specified target region in the genome to cause mutations (Feng et al., 2018). Heritable genomic alterations and transgene-free plants were produced as a consequence of the targeted plant genome editing made possible by CRISPR-Cas9 (Hu and Gao, 2023). Utilizing the CRISPR-Cas system several crops have shown both qualitative and quantitative gains (Zhang et al., 2020; Carrijo et al., 2021; Confalonieri et al., 2021; Eid et al., 2021; Oz et al., 2021; Zhang et al., 2021; Biswas et al., 2022; Lu and Tian, 2022). An overview of CRISPR- Cas technological advancements are shown in Figure 2 .

One of the major disadvantages of the CRISPR-SpCas9 gene editing system is the formation of off-target cleavage sites, that occurs as a consequence of the gRNA complexing with mismatched complementary target DNA within the genome. Several modifications have been made to the Cas9 enzyme to reduce off-targeting and increase target selectivity (Lee et al., 2019). CRISPR-CpfI is one of the orthologs of CRISPR-Cas9 discovered to enhance target selectivity. It was formerly known as Cas12a and is a Prevotella and Francisella-derived class II type V endonuclease (Zetsche et al., 2017). CRISPR-Cpf1 (Cas12a) from Alicyclobacillus acidoterrestris is a novel member of CRISPR-Cas system. Presently, AsCpf1, LbCpf1, and FnCpf1 are the most common types of Cpf1 used in genome editing. CpfI, in contrast to Cas9, requires just a single guide RNA (crRNA) to recognise its target, and it may target the A/T-rich area of the genome, expanding the number of sites it can change. Cas9, on the other hand, exclusively targets G-rich PAM sequences (Tang et al., 2016). Cas9 endonuclease cleaves the genome into blunt ends, while Cpf1 generates cohesive ends that are effective for gene insertions in plants (Yin et al., 2017). The CRISPR-Cpf1 system has endonuclease activity, and can induce DSBs, also have RNase III activity for pre-CRISPR RNA processing and doesn’t need tracrRNA. Furthermore, since a single crRNA array transcript may target several genomic locations, Cpf1 allows multiplexed genome editing. The CRISPR-Cpf1 system in monocot and dicot plants makes it easier to delete, insert, edit, and tag genes with fewer off-target effects. Recently, both model plants and crops, have exploited Cpf1s for crop genome editing (Endo et al., 2019; Wolter et al., 2019; Alok et al., 2020). The CRISPR-LbCpf1 system was used to edit the genome of allotetraploid cotton with an editing efficiency of 87% and no off-target effects were observed. CRISPR-Cpf1 in combination with the CRISPR-Cas9 system may activate the targeted gene knock-in mechanism for crop improvement (Kaul et al., 2020). Cpf1 has constraints, such as a shorter crRNA and specific temperature requirements for genetic transformation in plants (Lee et al., 2019; Schmidt et al, 2020). The shorter length of Cpf1 crRNAs compared to Cas9 may result in the formation of unwanted secondary structures and decreased efficiency, as shown in maize (Malzahn et al., 2019). The temperature sensitivity of Cpf1-mediated genome alteration in plants is a major disadvantage. The effects of low temperature on the activity of AsCpf1, FnCpf1, and LbCpf1 have been found in arabidopsis, rice, and maize (Kumar et al., 2020).

The CRISPR-Cas9 system can be used to knock out or insert new genes, however this method cannot change the sequence of DNA at the base level. Because of these restrictions, different methods are needed to achieve stable and accurate genome editing in plants. Recently, a breakthrough method known as “base editing” has evolved that permits exact nucleotide changes in a programmed way without causing gene disruption or necessitating a donor template (Komor et al., 2016). DNA base editors, which are generally consisting of a catalytically inactive nuclease linked to a base-modifying catalytically active enzyme, are used in base editing systems ( Figures 3A, B ). Currently, two DNA base editors are in use: the cytidine base editor (CBE) and the adenine base editor (ABE). Cytosine (C) is deaminated using CBE to produce uracil (U). During DNA replication, uracil (U) is read as thymine (T). As a result, CBE causes a single base shift from C:G to T:A (Komor et al., 2016). The inactive CRISPR-Cas9 domain in ABE is linked to adenosine deaminase, which helps to convert adenine (A) to inosine, which is read as guanine (G) during DNA replication ( Figure 3B ). As a result, ABE causes A:T to G:C base substitutions (Nishida et al., 2016). The first generation base editor was created by combining cytidine deaminase with Cas9 nickase (nCas9) (Komor et al., 2016). This base editing system comprises of a single-guide RNA with nCas9 to target the site and deamination on the non-complementary strand, as well as a single break in the target DNA strand to initiate the conversion of G to A on the opposite strand through DNA replication (Kumlehn et al., 2018). The base excision repair activity eliminates uracil via uracil N-glycosylases (UNGs). As a result of this, the BE1 technique is inefficient for altering single bases in vivo (Komor et al., 2016). Furthermore, the restricted number of targets is constrained by the short base editing window and the necessity of certain PAM sequences. Given these limits, as well as its low efficiency, a second-generation base editor (BE2), was created as an improved version of base editors (Wang et al., 2017). BE2, a novel base editing tool was created by using uracil DNA glycosylase to disrupt the excision repair process and boost editing efficiency by thrice. Moreover, BE2 produces very few indels (0.1%) during base editing, making it a great choice in instances where indels are unwanted (Komor et al., 2016). BE3 is a third-generation editor composed of rat cytidine deaminase APOBEC1 linked to a Cas9 nickase [nCas9 (D10A)] and the UGI. BE3 is required for the conversion of targeted cytidine to thymidine in DNA, and its PAM efficiency and specificity have been improved (Hess et al., 2016). Unlike cytidine deaminases, adenine DNA deaminases do not occur in nature. Significant protein engineering and directed evolution were used to construct ABEs using Escherichia coli TadA (Liu et al., 2017). TadA is a tRNA adenine deaminase from E. coli that converts adenine to inosine in the anticodon loop of tRNA Arg. It is similar to APOBEC. The first ABEs were created by fusing a TadA with a catalytically defective CRISPR-Cas9 mutant (Gaudelli et al., 2017). ABE7.7, ABE7.8, and ABE7.9 are the most active and sequence-compatible ABEs. The seventh-generation ABEs (ABE7.10) were proposed for efficient and pure A:T to G:C conversion in a wide range of targets. ABEs easily generate point mutations and programme all four transitions (C to T, A to G, T to C, and G to A), significantly increasing base editing. SNPs and minor indels cause most agronomically relevant features which BEs can repair for precision crop breeding (Zhao et al., 2011; Maccaferri et al., 2015; Wu et al., 2021). Previous research has proved the efficient use of base editors (ABE or CBE) in a vast array of plant species (Shimatani et al., 2017 and Zong et al., 2017; Kang et al., 2018; Li et al., 2018; Tian et al., 2018; Yan et al., 2018; Zong et al., 2018; Endo et al., 2019).

Prime editing is a unique, sophisticated, multifunctional, and precise genome editing method that generates all potential transition and transversion mutations as well as small indels in the DNA (Anzalone et al., 2019). Using novel prime editing method genetic information at the target loci has been successfully incorporated into the genome without DSBs. This approach employs the single-guided RNA referred known as primary editing gRNA (pegRNA) and the fusing of reverse transcriptase to the C-terminal of a Cas9 H840A nickase. The pegRNA may be utilized as a reverse transcription template (Vu et al., 2020). It is a modified sgRNA that incorporates a primer binding site (PBS) and the genomic sequence to be copied at its 3’ end ( Figure 3C ). Reverse transcriptase uses the nicked strand-free 3’OH group as a template to transcribe the genetic material from the pegRNA 3’ end extension (Vu et al., 2020) Nucleotide substitutions during DNA repair may occur if pegRNA were synthesized using modified nucleotides. Prime editing does not require the donor DNA during homologous DNA repair (HDR) process. Prime editor 1 (PE1), one of three basic editing techniques, has a Cas9H840A nickase with a wild-type Moloney murine leukaemia virus RT fusion at the C-terminus (M-MLV-RT) (Zhan et al., 2021). Prime Editor 2 now includes the proposed M-MLV-RT pentamutant (D200N/L603W/T330P/T306K/W313F), which has improved thermostability, processivity, DNA-RNA substrate affinity, and inhibited RNase H activity (PE2). In comparison to PE1, this system improved point mutations by 1.6 to 5.1-fold and consistently generated more efficient targeted insertions and deletions. In the PE3 method, two paired DNA strands are double-nicked, which could result in a DSB and indel creation as a result of NHEJ repair. Several parameters, including reverse transcriptase type, temperature, template length, PBS length, and the necessity for a second nick, may influence prime editing efficiency in plants. Prime editors have been shown to be quite helpful for both basic biological research and crop breeding. The upgraded prime editors have much lower off-target frequency in plants than the base editors (Jin et al., 2019). Multiple independent studies have shown that PE has a poor effectiveness for modifying the genome of plants (Xu et al., 2019; Butt et al., 2020; Jiang et al., 2020; Lin et al., 2020; Lu et al., 2020; Tang et al., 2021). These experiments demonstrate that PE has the potential to become a viable method for precise genome editing in crops, but more work has to be done to increase the efficiency of prime editing before this technology can be used more widely. PEs may execute targeted saturation mutagenesis to create beneficial genetic variants with increased agronomic performance for crop genes lacking functional SNPs (Molla et al., 2021).

One of the primary targets of heritable alterations in gene expression and cellular function is epigenetic modification. Targeted gene expression control was made possible by methylating DNA, creating non-coding RNAs and modifying histones (Kumar and Mohapatra, 2021). Addition of a methyl group to the C’5 position of cytosine residues is the hallmark of DNA methylation, a well-known epigenetic change associated with gene silencing. Histone modifications include acetylation, methylation, ubiquitination, and phosphorylation of amino acid residues in the N-terminal tail (Zhan et al., 2021). There are enzymes (writers) that catalyse the addition of epigenetic markers, such as DNA methyltransferases (DNMTs), histone acetyltransferases (HATs), and histone lysine methyltransferases (HKMTs), and enzymes (erasers) that catalyse their removal, such as DNA demethylases, histone deacetylases (HDACs), and histone demethylases (Zhang et al., 2021). To dynamically modulate epigenetic modifications, which in turn affects chromatin conformation and transcriptional regulation, writers and erasers must interact (Jaenisch and Bird, 2003; Torres and Fujimori, 2015; Feinberg et al., 2016). However, in very rare circumstances, epigenetic changes may cause a permanent change in gene expression without affecting the genome sequence. These epimutations are called epigenetic alterations. Transgenerational inheritance of changes in DNA methylation is the primary source of the matching epigenetic allele, often known as a “epiallele” (Hauser et al., 2011; Quadrana and Colot, 2016; Lloyd and Lister, 2022). Floral organ development (Jacobsen and Meyerowitz, 1997), flowering time (Soppe et al., 2000), starch metabolism (Silveira et al., 2013), fruit ripening (Manning et al., 2006), vitamin E accumulation (Quadrana et al., 2014), and sex determination are just some of the many developmental/physiological processes that have been shown to be regulated by epialleles in plants (Martin et al., 2009). The ability to regulate transcription without changing DNA sequences has led to the development of epigenome editing technologies. Epigenome editing, the targeted modulation of epigenetics at a single gene, is often accomplished by fusing a specific epigenetic modifier with a targeting module of the standard genome editing system. The catalytic domain of an epigenetic modifier may be linked to programmable DNA-binding modules like ZF, TALE, and catalytically inactive Cas9 (dCas9). Epigenome editing has great promise as a means of controlling gene expression in a targeted manner. Epigenetic alteration in plants may lead to the generation of new epialleles linked to desirable features for crop development. Several reports have been made on investigations of epigenetic alterations in plants that are specifically targeted (Gallego-Bartolomé et al, 2018; Lee et al., 2019; de Melo et al., 2020). An effective technique for crop improvement might be epimutations associated with desirable features. Understanding the fundamentals of how epigenetics influences yield, quality, disease resistance, and stress tolerance that are vital to farmers and industry necessitates the research of epimutations across a broad variety of crop species. Epigenome editing will help future crops to adapt with harsh environmental circumstances. Earlier it has been demonstrated that the conserved histone variant (H2A.Z) is required for transcriptional control, defensive responses, and plant growth, development, and flowering. Using CRISPR-Cas9 mediated approach in tomato, Slhta9, Slhta11 double-mutant and Slh2a.z were created and discovered that these mutations reduced the fresh weight of tomato fruits. mRNA-seq data demonstrated that genes SlPSY1, SlPDS, and SlVDE, which encode important enzymes in the production pathway of carotenoids, were considerably elevated in later ripening stages, commensurate with the enhanced carotenoids in slh2a.z double-mutant fruits. Previous research has reported that Slh2a.z regulates carotenoids and offers a resource for studying Slh2a.z-dependent gene expression (Yang et al., 2021b). While, epigenome engineering has the potential to be a game-changing tool for the precise regulation of epigenetic markers, further research is required to fully realise its potential.

In multiplex genome editing, many targets, both related and unrelated, can be simultaneously edited. This feature of CRISPR-Cas provides several applications such as targeting multiple members of a gene family, simultaneous targeting of many closely related sequences, numerous alleles of a single gene and homoalleles in polyploid plants (Li et al., 2013). Several gRNAs were inserted as polycistronic transcripts or separate expression cassettes with their own promoters, and they were processed into mature gRNAs by either endogenous or exogenous nucleases, the CRISPR-Cas9 system makes the latter approach very effortless. The most prevalent method for the generation of numerous gRNAs is to express each individual gRNA using its own promoter and terminator, a strategy that may be implemented using either distinct plasmids for each gRNA or by connecting several cassettes on the same vector (Xing et al., 2014; Ma et al., 2015). Nonetheless, many studies have reported the delivery of gRNAs and Cas9 as ribonucleoprotein complexes, and this strategy might potentially be utilized to carry multiple gRNAs (Woo et al., 2015; Liang et al., 2017). In order to generate many gRNAs from a single transcript, which is then processed to generate the mature gRNAs, a number of alternative techniques have been devised. Like CRISPR, the Cys4 system employs a bacterial endonuclease to edit DNA. Here, several gRNAs are placed in tandem, interspersed with Cys4 recognition sites; this allows for post-transcriptional release of mature gRNAs upon coexpression of Cys4 (Cermak et al, 2015). The CRSIPR/Cpf1 approach is an option that has the benefit of using the same enzyme (Cpf1) to separate the polycistronic gRNAs and to target the genomic loci for alteration (Wang et al., 2018). Thirdly, self-cleaving ribozymes may be employed to liberate an internal gRNA from a ribozyme-gRNA-ribozyme complex (Tang et al., 2016). Compare to this, the tRNA-dependent gRNA method has been used in many studies for multiplex genome editing in plants. All organisms manufacture tRNA as a longer precursor, which is then digested by endogenous RNases to form the mature tRNA. The creation of progRNA arrays with target sites for tRNA maturases allows the automated release of individual gRNAs (Hashimoto et al., 2018). The first emphasis of multiplex genome editing in plants was on input traits like herbicide resistance, and more over a hundred simultaneous targeting events have been documented. Since then, this discipline has grown to incorporate plant growth, metabolic engineering, hormone sensing and biosynthesis, among other significant characteristics. The genes OsGSTU, OsMRP15, and OsAnP, which are involved in anthocyanin transport and accumulation, were concurrently changed in a rice line with purple leaves to produce mutants with green leaves. The OsWaxy gene, which makes granule-bound starch synthase, is also targeted by the same researchers. They recovered plants with mutations in one or two but not all three loci, and the mutants’ amylose content dropped from 14.6% to 2.6% (Ma et al., 2015). Six gRNAs were used to target five genes in the tomato carotenoid biosynthesis pathway, with two targets in the SGR1 gene and one target each in the genes LCY-E, Blc, LCY-B1 and LCY-B2. By promoting the formation of lycopene and preventing its conversion to β- and α-carotene, the lycopene content of tomato fruits was intended to be increased (Li et al., 2018)

The primary objective of plant gene editing is to create alterations to the genome that are stable, heritable, and nonmosaic, allowing the resultant trait or phenotype to be consistently preserved and passed on to future generations (Huang et al., 2022). Thus, when the intended gene editing has been performed, all traces of the gene-editing components must be eliminated (Metje-Sprink et al., 2019; Miroshnichenko et al., 2019). Recent advancements in genome editing tools including DNA-free delivery methods and base editing systems solved this problem and provide a wide opportunity to edit plant genomes in a precise manner (Bhattacharjee et al., 2020). Advanced tools such as direct delivery method, and delivery of in vitro assembled ribonucleoprotein (Cas9/gRNA) and use of virus-like particles and employment of bacterial secretory systems for Cas/gRNA delivery are the main approaches that have been employed to accomplish DNA-free genome editing. Such complex formulations can be delivered into plant cell by agrobacterium mediated approach, protoplast transformation, microinjection, electroporation, particle bombardment and liposomes mediated transformation (Zhang et al., 2017). Transient transfection may be used to directly insert CRISPR ribonucleoprotein (RNP) or plasmids with the Cas and sgRNA sequences into protoplasts, enabling the regeneration of recombinant DNA-free plants without raising GMO concerns (Hsu et al., 2021). Furthermore, in order to attain DNA free genome editing, CRISPR-Cas9 RNPs were employed for targeting two endogenous genes (FRI and PDS) in cabbage and Chinese cabbage protoplasts. As a result of this, local insertion and deletion mutations (indels) were obtained (Murovec et al., 2018). To enable DNA-free genome editing in canola, RNPs (composed of LbCas12a and a single guide RNA) were transformed using polyethylene glycol (PEG) with a mutation frequency of 40% protoplast derived plants (Sidorov et al., 2022). Although, DNA-free genome editing using protoplast is simple, efficient, and transferable in a generation but in some monocot crops, the protoplast isolation and regeneration of shoots remain a bottleneck. Besides this particle bombardment mediated DNA-free genome editing is restricted to some species due to resulting in cells rupturing, therefore, the plant transformation method needs to be optimized for achieving success in this system.

The dependency on plant genetic transformation and regeneration processes is a big barrier for many species when it comes to gene editing. The most prevalent method of gene delivery is agrobacterium-mediated transformation, which involves integrating the DNA to be transferred into its transfer DNA (T-DNA), which is then integrated into the plant genome. Particle bombardment using a gene gun is another method used in monocot species. Both procedures result in the random integration of DNA into plant genomes (Laforest and Nadakuduti, 2022). Foreign DNA insertion into host DNA is deemed genetically modified and needs regulatory oversight. Plant cell walls, pose a specific barrier to the introduction of gene-editing reagents. Non-transgenic genome editing is possible in protoplasts, which, like animal cells, lack cell walls. Protoplast transfection using plasmids containing gene-editing agents or RNPs has resulted in the regeneration of complete plants in a few species (González et al., 2020; Laforest and Nadakuduti, 2022). However, complete regeneration of plant from single-celled protoplasts, necessitates extensive tissue-culture procedures that result in frequent and undesirable somaclonal variation. A recent study of protoplast regenerants identified anomalies in chromosome structure and number that might impact plant phenotype (Fossi et al., 2019). As a result, innovative ways for overcoming these issues, particularly those that do not rely on tissue culture, seems useful (Ji et al., 2020) and are discussed below.

Recent consumer trends reflect a greater appreciation for the nutritional value of food grain, fruits and vegetables. As economic growth continues, the demand for premium grain quality increases. In wheat, four grain quality related genes were targeted by genome editing: pinb, waxy, ppo, and psy, which are involved in wheat grain hardiness, starch and dough colour, respectively. As a result, novel wheat germplasms with enhanced grain quality in terms of hardness, starch content, and dough colour were produced (Zhang et al., 2021). In rice (Oryza sativa L.), the diverse actions of natural Wx alleles govern distinct amylose contents (AC), gel consistency (GC), and pasting viscosity of grain starches; these parameters together affect grain appearance, cooking/eating quality, and starch physical properties (Zhang et al., 2019). Quantitative modulation of Waxy allele expression by CRISPR-Cas9-based promoter and 5’UTR-intron editing increases rice grain quality and the quality of rice products to satisfy customer’s preference (Zeng et al., 2020). Grain size is one of the primary characteristics that impact the productivity and quality of rice grains. The GS3 gene is the first significant quantitative trait locus (QTL) found to regulate the length and weight of rice grains. CRISPR-Cas9 gene-editing technology was used to introduce an altered gs3 allele into the indica maintenance line Mei1B in order to improve its grain production and quality (Huang et al., 2022). Luo et al. (2022) were the first to produce high-amylose cassava by CRISPR-Cas9-mediated mutation of the starch branching enzyme gene SBE2. Thus, CRISPR technology was proved to be a successful method for creating new starch types for food and commercial purposes. Cereals, high in resistant starch (RS) may be beneficial to health and might help to protect protect against chronic diseases that are linked to poor nutrition. Multiplex CRISPR–Cas9 gene editing of starch branching enzyme genes boosted ‘Presidio’ rice’s resistant starch. With the help of agrobacterium-mediated transformation, the CRISPR-Cas9 vector construct containing four sgRNAs targeting the SBE gene was introduced into the American rice cultivar Presidio. Eight transgene-positive T0 plants had all four SBE gene knockout variants. SBE-edited lines have up to 15% more RS than the wild-type (WT) cultivar Presidio (Biswas et al., 2022). Mutagenesis of starch biosynthetic genes in sweet potatoes (Ipomoea batatas) using CRISPR-Cas9 was used to enhance starch quality (Wang et al., 2019).

Enhancement of nutritional content and reduction of anti-nutrition factors in food crops is among preferred consumer’s trait. Non-proteinogenic amino acid -aminobutyric acid (GABA) has been shown to reduce blood pressure in hypertensive individuals who consume it orally. An increase in GABA levels may further improve the blood pressure reducing effect of tomato fruit. Using CRISPR-Cas9 technology, researchers knocked out an autoinhibitory domain of Glutamate decarboxylase (GAD) genes (a crucial enzyme in GABA production) such as SlGAD2 and SlGAD3 in tomato to boost its GABA content (Nonaka et al., 2017). Oils extracted from seeds have both culinary and industrial uses. In contrast to monounsaturated fatty acids (MUFA) most seed oils are high in polyunsaturated fatty acids (PUFAs). Soybeans had their FAD2-2 microsomal omega-6 desaturase specifically disrupted using the CRISPR-Cas9 nuclease technology (Glycine max.L). The oleic acid content of the edited lines varied significantly, reaching a maximum of 65.58% whereas the linoleic acid concentration reached a minimum of 16.08% (Al Amin et al., 2019). Three homologs of the gene for fatty acid desaturase 2 (FAD2), the enzyme that converts oleic acid into linoleic acid, have been found in the genome of the new oilseed crop camelina. Knockout plants lacking the CsFAD2 gene were created using CRISPR-Cas9 to boost the MUFA content of Camelina seed oil (Lee et al., 2021). Improvement of the fatty acid composition in Rice bran oil (RBO), OsFAD2-1 gene was disrupted by CRISPR-Cas9-mediated targeted mutagenesis. Homozygous OsFAD2-1 knockout mutant rice plants have increased oleic acid compared to wild type (Abe et al., 2018). The breeding of soybean to lower its saturated fatty acid (FA) content, which has been related to cardiovascular disease, would have a significant impact on nutritional enhancement. Obtaining CRISPR-Cas9 mutants that lack the GmFATB1 (Acyl-acyl carrier protein thioesterases) gene resulted in a shift in oil profile, indicating that there is considerable potential for increasing the quality of soybean oil for human health (Ma et al., 2021). The processing and nutritional value of vegetable oils might be negatively impacted by the presence of erucic acid (EA). That’s why brassica with low EA has been a desirable feature for generations. The enzyme fatty acid elongase 1 (FAE1) plays a crucial role in the production of EA. Three Brassica napus germplasms (high EA (>30%) and oil (>50%)) were mutated specifically in two homologous copies of BnaFAE1 utilising CRISPR technology. In BnaFAE1-edited germplasms, EA content was dramatically decreased, whereas oleic acid content was elevated to varying degrees (Liu et al., 2022).

Consumer acceptance of a crop is determined by factors other than its nutritional content, such as sensory qualities (i.e., colour, texture, and flavour). Appearance is the primary determinant of consumer preference, whereas other aspects maintain future purchases. The market for horticultural crops, such as fruits, vegetables, and floricultural crops, places a premium on such sensory qualities. Diverse crops or their harvests provide a spectrum of hues (e.g., green, yellow, red, purple, or multicolour), flavours (e.g., neutral, slightly sour, spicy, or bitter), and textures. Among horticulture/ornamental crops, visual aspect and fragrance are important elements for overall plant quality and novel flower colour production is regarded as one of the chief commercial demand of consumers. Other sensory characteristics, such as aroma and texture/vase life, are also highly prized for their aesthetic and economic potential. Therefore, generation of crop varieties with desired colour, improved fragrance, and rich texture using gene editing technology may become prospective benefit to both growers and consumers.

It is generally well known that the various secondary metabolites of medicinal plants have therapeutic capabilities. Gene editing holds potential for enhancing the consumer traits with regard to the production of medicines and therapeutics from medicinal plant species. Salvia miltiorrhiza, a medicinal plant, has been effectively edited by employing CRISPR-Cas9 driven knock out of the diterpene synthase gene (SmCPS1) involved in tanshinone production without interfering with other phenolic compounds (Li et al., 2017). This will undoubtedly open the way for large-scale genome editing in S. miltiorrhiza, with the goal of elucidating the process for secondary metabolite production, improving quality, and increasing yields of this important traditional Chinese medicinal plant. Camptothecin (CPT) is a natural chemical with outstanding anticancer effects. Ophiorrhiza pumila produced camptothecin, its concentration in hairy root lines mediated by CRISPR-Cas9 system (OpG10H or OpSLS knock-out lines) was clearly decreased, showing that both OpG10H and OpSLS play crucial roles in CPT biosynthesis. Laccases, which are involved in the synthesis of significant medicinal phenolic acid compounds like salvianolic acid B (SAB), used to treat cardiovascular disease, are another attractive target for gene editing in salvia. Multiple laccase genes in salvia were targeted using the CRISPR-Cas9 technology, leading to a significant reduction in target laccase gene expression and phenolic acid in gene edited lines. There is a significant function for rosmarinic acid, a kind of aqueous phenolic compounds, in the treatment of inflammatory illnesses. Improved rosmarinic acid quality has resulted from the use of CRISPR to silence the rosmarinic acid synthase (SmRAS) gene in salvia. This has enhanced synthesis of 3, 4-dihydroxy phenyl lactic acid (Zhou et al., 2018).

The reduction in lignin content and modification of its composition in cell wall of plants increase the appropriateness of lignocellulosic biomass for the pulp, paper, and textile industries, biofuel and feed (Verma and Dwivedi, 2014; Capstaff and Miller, 2018). Genetic modification can reduce lignin content and enhance saccharification efficiency, but usually at the cost of moderate-to-severe growth penalties. A single DNA construct was employed in CRISPR-Cas9 gene editing to knock out expression of an endogenous gene of lignin monomer biosynthesis while simultaneously expressing a modified version of the gene’s open reading frame that escapes cleavage by the Cas9 system and complements the mutation in a tissue-specific manner. By expressing the complementary open reading frame in vessels, it is possible to regenerate arabidopsis plants with less lignin, wild-type biomass production, and up to a fourfold increase in cell wall sugar yield per plant (Yu et al., 2021). It has been reported that the CRISPR-Cas9 system was used to target two 4CL (4-coumarate: CoA ligase) genes, 4CL1 and 4CL2, involved in lignin and flavonoid biosynthesis, respectively, in the woody perennial Populus, with 100% mutation efficiency for both genes and biallelic modifications in every transformant tested (Zhou et al., 2015). CRISPR-Cas9-mediated mutation of HvCOMT1, the lignin biosynthetic gene that forms lignin syringyl units, reduces barley’s lignin content (Lee et al., 2021). Caffeoyl shikimate esterase (CSE), which helps plant to synthesize lignin, is a prospective target for improving lignocellulosic biomass crops for sustainable biofuel generation. CRISPR-Cas9-mediated knockouts of each CSE gene in transgenic hybrid poplars improved saccharification efficiency by reducing lignin content (Jang et al., 2021). Natural rubber (NR) is an important raw material for a large number of industrial products. The primary source of NR is the rubber tree Hevea brasiliensis, but increased worldwide demand means that alternative sustainable sources are urgently required. As a natural rubber substitute, the roots of the Taraxacum kok-saghyz (TK-Rubber Dandelion) plant generate a high-molecular-weight rubber. As inulin is thought to be counterproductive to rubber production, the domestication of TK was hastened by targeting the fructan:fructan 1-fructosyltransferase (1-FFT) gene, which encodes an enzyme involved in inulin manufacture, using the CRISPR-Cas9 gene-editing tool (Iaffaldano et al., 2016). Linum usitatissimum L., a worldwide cash crop, produces linseed oil and linen. High temperature stress limits flax growth. The studies of Saha et al. (2019) identified 34 flax genome-wide HSF genes and created guide RNA sequences for gene editing with minimal off-target consequences. Genome engineering LuHSFs will create high-temperature stress-tolerant flax cultivars and boost fibre output. Using the CRISPR-Cas9 technology, targeted mutations in GhALARP (a gene primarily expressed in cotton fibres that encodes an alanine-rich protein) have been produced (Zhu et al., 2018). These mutants provided evidence for deducing the role of GhALARP in the formation of cotton fibre.

Mitochondria and chloroplasts, having their individual genomes that encode many genes required for biological processes such as respiration and photosynthesis, respectively. The lack of efficient ways for targeting DNA in plant organelles has hampered plant organelle gene editing, which is an unmet need in plant biology fundamental research (Kang et al., 2021). Methods for editing these genes in organelles are in great demand for elucidating their functions and improving vital traits. For instance, targeted mutagenesis in the mitochondrial atp6 gene may result in male sterility (Howad and Kempken, 1997), which is a beneficial trait for crop breeding, while a precise point mutation in the 16S rRNA gene of the chloroplast genome results in antibiotic resistance (Fromm et al., 1989). For crop improvement, CRISPR-Cas has been utilized to edit nuclear genomes. These techniques have never been used to target higher plant plastid genomes. Despite the fact that there are no nuclease-mediated genome editing approaches for plastome engineering, homologous recombination-based plastid transformation may introduce point mutations (Bock, 2015). The biggest hurdle in using CRISPR-Cas mediated mtDNA and cpDNA editing is introducing both sgRNA and Cas9 endonuclease into these double-membraned organelles (Glass et al., 2018). Even though, mitochondrial genome editing with CRISPR-Cas9 have been reported in human cells, but the results aren’t clear (Yang et al., 2021a). Optimising CRISPR-Cas system organelle genome editing in multicellular plants will definitely make it a very useful tool for crop improvement programme. To improve CRISPR-Cas9 system for editing mitochondrial DNA a modified sgRNAs that can target mitochondria should be used. This is done by adding the RNA transport-derived stem loop element (RP-loop) to the sgRNA, which lets it transport into mitochondria. This resulted into the reduced transcript levels of the targeted genes of mtDNA in human cells and revealed that sgRNA can introduced into organelles by using the native machinery for introducing RNA into organelles (Hussain and Kumar, 2021). But the ways that organisms bring RNA into organelles are very different and are mostly unknown in plants (Tian et al., 2018). Gómez and Pallas (2012) found that addition of an internal sequence of 110bp from the eggplant latent viroid to the 5′ end of the green fluorescent protein (GFP) mRNA caused this chimeric RNA to be imported into the chloroplasts of Nicotiana benthamiana. Recently Kang et al. (2021) used DddA-derived cytosine base editor (DdCBE)2 to induce point mutations in mitochondria and chloroplast DNA. This DdCBEs triggered base editing at rates of up to 25% (mitochondria) and 38% (chromosomes) in lettuce or rapeseed calli (chloroplasts). In order to prevent off-target mutations produced by DdCBE-encoding plasmids, researchers have also demonstrated DNA-free base editing in chloroplasts by delivering DdCBE mRNA to lettuce protoplasts. Using bacterial cytidine deaminase linked to the DNA-binding domains of transcription activator-like effector nucleases, targeted base editing in the Arabidopsis thaliana plastid genome has been accomplished. In certain plantlets of the T1 generation, the targeted Cs were homoplasmically swapped with Ts, and the mutations were acquired by their progeny regardless of the nuclear vectors delivered (Nakazato et al., 2022). To improve consumer specific traits like medicinal/industrial value in crops, editing of organelle genome is crucial as chloroplast genome harbors various biosynthetic pathways, including the shikimate, de novo fatty acid synthesis, and methylerythritol 4-phosphate pathways which serves as a precursor for a wide range of commercially important secondary metabolites, including tocopherols, pigments, and many phytohormones (Li et al., 2021). Still, few cues remain a challenge in organelles both chloroplast and mitochondria genome transformation such as having a double membrane, poor gene expression in non-green plastids and the limited host range of agrobacterium.

Polyploidy is the acquisition of one or more extra full chromosomal sets inside an organism. There are at least three distinct types of polyploidy: autopolyploids, allopolyploids, and segmental allopolyploids. Due to their huge genome size and repetitive sequences, polyploids genomes are challenging to sequence. Because many alleles must be edited concurrently, gene knockout effectiveness is often lower in polyploid plant species than in diploids (Ahn et al., 2020). An effective expression system and a highly active Cas nuclease are essential for successful polyploid plant genome editing. Multiallelic genome editing has been accomplished in various polyploid plant species, including both model systems and crop species till date (Weeks, 2017; Naim et al., 2018; González et al., 2020; Lin et al., 2020). In tetraploid potato, CRISPR-Cas9 base and prime editors have been used to alter the catalytic motifs of the GBSSI (granule-bound starch synthase) gene (Toinga-Villafuerte et al, 2022). Using CRISPR-Cas9 RNPs to induce mutations in the polyphenol oxidase 2 gene (StPPO2) in tetraploid potato led to a significant decrease in enzymatic browning following cutting (González et al, 2020). Earlier, genome editing through CRISPR-Cas9 for the improvement of grain quality (important trait for consumers) including grain and kernel weight and storability in hexaploid wheat have been studied by Dayani et al. (2019). PolysgRNAs tRNA-mediated genome editing may be used to specifically target the existence of multiple copy numbers in polyploidy crops. Furthermore, environmental stress, epigenetic alterations, physiological and cellular responses to stress contribute to a rise in polyploidy (Van de Peer et al., 2021). In an octaploid crop like strawberry, fruit colour affects consumer preference and is an important trait for breeding. Using CRISPR-Cas9 technology, Gao et al. (2020) knock out six of eight copies of the Reduced Anthocyanins in Petioles (RAP) gene in cultivated octaploid strawberry plants, resulting in altered fruit coloration. Multisite alterations in the genome are possible using CRISPR-Cas. In contrast to diploid crops, where building a CRISPR system is relatively straightforward, polyploid crops provide more of a challenge owing to the availability of many alleles of target genes. Moreover, accurate genome editing using HDR in stable transgenic lines of polyploid plants, in addition to gene knockdown, remains difficult. Thus to achieve mutations in all different alleles in polyploid crops, the design of guide RNAs require special attention. The simultaneous editing of numerous homologs using CRISPR-Cas technology without any mutations in the background would provide novel breeding opportunities for mutant genotypes.

Off targeting involves cleavage and genomic DNA modification outside the target region. Off targeting is a critical challenge when using CRISPR-Cas system to edit genes associated with traits (Wolt et al., 2016). Cas9-induced double-strand breaks may lead to significant deletions and genomic rearrangements. Several methods are available to detect the off-targets which includes deep sequencing, web-based prediction tools, FISH, HTGTS, IDLV, Digenome- seq, T7E1 assay, Guide -seq, and chip-seq (Cho et al., 2013; Ran et al., 2013; Heigwer et al., 2014; Tsai et al., 2015; Paulis et al., 2015). In plants, much research has been conducted to discover off-target impacts sequencing the whole genome of arabidopsis, rice, cotton, etc. Studies have revealed that genome editing in plants using wild-type Cas9 and Cas12a has remarkable specificity, revealing that the majority of mutations discovered in edited plants are the consequence of somaclonal alterations (Swarts and Jinek, 2018). CBEs, produce genome-wide off-target effects in rice, as proven by whole genome sequencing; hence, their usage may require extra screening and purifying selection (Doman et al., 2020). However, base editors may be designed to restrict their RNA editing activity significantly. To reduce the likelihood of off-target mutations, paired nCas9s might be utilized. High fidelity versions of SpCas9 have been developed via protein engineering to lower Cas binding affinity (and hence increase editing specificity). In rice, the on-target editing activity of eSpCas9 (versions 1.0 and 1.1) and SpCas9-HF1 is preserved, while the specificity is improved by using the t-RNA-sgRNA processing system (Huang et al., 2022). Two versions of Cas9, eHF1-Cas9 and eHypa-Cas9, have been shown to successfully alter the rice genome. Recent research indicates that xCas9 has greater targeting selectivity than wild-type Cas9 in rice (Zhang et al., 2019). However, due to the inherently reduced nuclease activity of many high-fidelity SpCas9s in plants, their use for plant genome editing is less reliable until additional advancements are made. Other strategies for minimising off-target effects include the development of gRNAs with fewer possible mismatch targets in a given genome. In addition, restricting the genome’s exposure to CRISPR reagents, such as through transient expression and RNP transformation, may lower the likelihood of off-target activity (Xu et al., 2019).

In terms of precision and efficiency, mutagenesis by CRISPR-Cas9 mediated genome editing outperforms spontaneous and induced mutations. Organelle genome editing, higher ploidy levels, complex genome/lack of genome sequence, and off targeting are important hurdles in improvement of commercially viable/consumer preferred traits in crops. But given the immense potential of genome editing, we anticipate that these problems will be solved in the near future.