Carotenoids are lipophilic isoprenoid molecules consisting of a polyene backbone that generally contains 3–11 conjugated double bounds. They can be synthesized de novo by plants, algae, photosynthetic bacteria, and many nonphotosynthetic microorganisms (Hirschberg, 2001; Fraser and Bramley, 2004; DellaPenna and Pogson, 2006; Moise et al., 2014; Rodriguez-Concepcion et al., 2018; Zheng et al., 2020a). Humans cannot build carotenoids de novo, but need them as important antioxidants and as essential source of provitamin A, particularly if animal-derived food is not available (Fraser and Bramley, 2004; DellaPenna and Pogson, 2006; Giuliano, 2014; Nisar et al., 2015; Zheng et al., 2020b).

In many crops and other plant species, carotenoids confer their vivid yellow to red colors to fruits, flowers, tubers, and seeds (Yuan et al., 2015; Giuliano, 2017), which attract animal pollinators and distributors and are important feature for consumers, which indicate the quality of plant products and, hence, promote their marketability. More importantly, carotenoids are essential for plant photosynthesis, as they protect the photosynthetic apparatus from photooxidative damage and contribute to light-harvesting (Hashimoto et al., 2016; Rodriguez-Concepcion et al., 2018; Moreno et al., 2021). The conjugated double bonds make carotenoids themselves susceptible to photooxidation and other unspecific oxidative breakdown processes, such as lipoxygenase mediated co-oxidation, as well as to targeted, enzyme-catalyzed cleavage of certain double bonds (Zheng et al., 2020a; Moreno et al., 2021). The term “apocarotenoid” is used to define the cleavage products arising from these processes. The large number of double bonds in carotenoid backbone and of different carotenoids leads to a plentitude of apocarotenoids with various physico-chemical properties and biological functions. The compound family of apocarotenoids includes precursors of important phytohormones, that is, abscisic acid (ABA) and strigolactones (SLs), signaling molecules involved in plant growth, development, and resistance against pathogens and herbivores (Jia et al., 2018; Felemban et al., 2019; Watkins and Pogson, 2020; Wang et al., 2020b), as well as apocarotenoid pigments and volatiles responsible for color and aroma of fruits, flowers, or vegetables (Liang et al., 2021; Zheng et al., 2021).

Plant apocarotenoids and their derivatives have also important functions in promoting human health (Rodriguez-Concepcion et al., 2018). Humans contain two carotenoid cleavage enzymes, i.e., β-carotene cleavage oxygenase 1 and 2 (BCO1 and 2), which convert some carotenoids into a restricted set of apocarotenoids, including vitamin A (Harrison, 2005; Von Lintig, 2010); however, they cannot produce many health-promoting apocarotenoids, such as crocins and safranal (Frusciante et al., 2014; Ahrazem et al., 2016b). Given the importance of apocarotenoids for plants and humans, numerous studies have been performed on investigating the biological functions of apocarotenoids, elucidating their biosynthetic pathways and the regulation of their formation. Naturally occurring inter- or intraspecies apocarotenoid variation was observed in some important crops, such as citrus, tomato, and Capsicum species (Gao et al., 2019; Zheng et al., 2019; Zoccali et al., 2021), which would help to decipher apocarotenoid biosynthesis and its regulation, and genetic control of apocarotenoid diversity. Nowadays, the advancements in global profiling strategies, such as genomics, transcriptomics, and metabolomics, and multi-omics approaches have been very helpful for investigating the variation of apocarotenoid levels as well as the underlying genetic mechanisms. Several recent reviews cover the biosynthesis of apocarotenoid-derived hormones and signaling molecules and their roles in plant development and growth (Felemban et al., 2019; Fiorilli et al., 2019; Wang et al., 2020b; Moreno et al., 2021). In this review, we will mainly focus on the advances that have recently been made in understanding the regulation, metabolism, and genetic mechanism underlying apocarotenoid diversity, particularly apocarotenoid pigments and volatiles. We also depict the possible impact of understanding the mechanisms behind apocarotenoids diversity on accelerating molecular breeding and CRISPR-based next-generation metabolic engineering of apocarotenoids for crop improvement.

Non-enzymatic and enzymatic oxidative cleavage of carotenoids and further metabolism of thereby arising apocarotenoids lead to various biologically important plant metabolites, including phytohormones, pigments, volatiles, and signaling molecules (Table 1). Due to their important roles in many physiological, developmental processes, and plant-biotic environment interactions, the phytohormones ABA and SLs are best-known examples for biologically important plant apocarotenoids. ABA is widely distributed in nature and is common in cyanobacteria, algae, fungi, plants as well as in animals (Wang et al., 2020b). It plays important roles in many physiological and developmental processes, including the establishment of seed dormancy, root, and shoot growth and regulation of stomatal closure, and is a major component in plant abiotic and biotic stress response (Chen et al., 2020; Moreno et al., 2021). SLs were originally discovered as the host-derived rhizospheric chemical signal that induces seed germination in root parasitic plants, such as Striga hermonthica, which represent a major threat to global food security (Xie et al., 2010; Jamil et al., 2021). Later studies on arbuscular mycorrhization revealed the role of SLs as the plant-derived signal that induces hyphal branching in AM-fungi, which paves the way for host colonization (Cook et al., 1966; Lanfranco et al., 2018; Wang et al., 2020b). Within plants, SLs inhibit shoot branching and the formation of adventitious roots, stimulate internodes elongation, and increase stem thickness (Al-Babili and Bouwmeester, 2015; Moreno et al., 2021). Furthermore, SLs are involved in leaf and floral organ senescence, as well as in biotic and abiotic stress response (Al-Babili and Bouwmeester, 2015; Decker et al., 2017; Wang et al., 2020b). Besides these two apocarotenoid-derived phytohormones, recent studies on the formation and biological functions of apocarotenoids unraveled new carotenoid-derived plant signaling molecules and growth regulators. For example, Wang et al. (2019a) identified a new growth regulator named zaxinone, which promotes rice growth and modulates its hormone homeostasis. Exogenous application of zaxinone as well as of zaxinone mimics increased the growth of crown roots of rice seedlings and alleviated Striga-infestation in a Striga-susceptible rice cultivar, by reducing SL content and release (Wang et al., 2019a, 2020a), demonstrating its application potential for improving rice growth and combating root parasitic weeds. Very recently, Wang et al. (2021) also demonstrated that increased sugar uptake and metabolism is likely the major reason for growth-promoting activities of zaxinone in rice and that zaxinone effect on root cell division activity in root meristem and on the number of cortex cell layers is likely caused by modulating cytokinin content. The enzyme involved in zaxinone formation in rice, the carotenoid cleavage dioxygenase (CCD) Zaxinone synthase, is common in mycorrhizal plants, but absent in those, such as Arabidopsis thaliana, that do not build this symbiosis (Wang et al., 2019a). In contrast to rice, a recent study showed that zaxinone application to hydroponically grown Arabidopsis seedlings increased SL and ABA content in roots and did not improve growth. This result suggests that the activities and functions of zaxinone may differ between plant species and are likely coupled to their ability to establish arbuscular mycorrhizal fungi (AMF) symbiosis (Wang et al., 2019a; Ablazov et al., 2020; Moreno et al., 2021).

Oxidative cleavage of carotenoids at more than one double bond results in the production of various dialdehyde products. Jia et al. (2021a) identified a carotenoid-derived C₁₀-dialdehyde that was named anchorene, which specifically promoted the growth and development of Arabidopsis anchor roots. This type of roots emerges from the collet region, the root–hypocotyl junction, through modulating auxin homeostasis. The identity of anchorene as a natural carotenoid-derived metabolite was confirmed using mutants affected in carotenoid biosynthesis and chemical inhibitors of carotenoid biosynthesis and by establishing a LC-MS-based system for isolating and detecting of carotenoid-derived dialdehydes (Jia et al., 2019; Mi et al., 2019). The discovery of anchorene unraveled, for the first time, a biological function of carotenoid-derived dialdehydes in plant development.

β-Cyclocitral (β-CC) is a volatile apocarotenoid derived from the oxidation of carotenoids (e.g., β-carotene), which is widely present in nature and is common in various organisms ranging from cyanobacteria and fungi to plants. It was shown that β-CC is a retrograde signaling molecule regulating the expression of oxidative stress-responsive genes, resulting in acclimation of plants to high-light conditions (Ramel et al., 2012). β-cyclocitric acid (β-CCA), a direct oxidation product of β-CC, is also a stress signal that enhanced drought tolerance likely through a signaling pathway different from that of β-CC (D'alessandro et al., 2019). Because of its water-soluble character, β-CCA is more suitable for agricultural application and can be more easily used to increase drought tolerance of crops through irrigation or by spraying, compared with its precursor β-CC (Havaux, 2020). In addition to its role in oxidative stress response, β-CC is a root growth regulator. Exogenous application of β-CC promoted the growth of primary roots in Arabidopsis, tomato, and rice. It was also shown that β-CC alleviates the inhibitory effect of salt on the growth of rice roots (Dickinson et al., 2019). A recent study showed that β-CC induces plant resistance against Spodoptera littoralis, as an herbivore-triggered signal, and downregulates the methylerythritol-4-phosphate (MEP) pathway flux, which is responsible for plastid isoprenoid biosynthesis, through direct inhibition of 1-deoxy-D-xylulose-5-phosphate synthase (DXS), a rate-limiting enzyme of the MEP pathway (Mitra et al., 2021). Other volatile apocarotenoids are also involved in herbivore resistance or response. For example, β-ionone is released as herbivores-induced defense volatile in canola, Brassica napus, and shows a strong repellent effect toward both the spider mite and flea beetle (Cáceres et al., 2016). α-Ionone, a structural isomer of β-ionone, induces jasmonic acid (JA) independent resistance to western flower thrips in tomato and Arabidopsis, by reducing the survival rate of western flower thrips without exhibiting direct insecticidal activity (Murata et al., 2020). Loliolide, a C₁₁-terpene lactone, functions as an endogenous signaling metabolite that induces resistance to multiple herbivores, such as the two-spotted spider mite, common cutworm, and western flower thrips, possibly independent of JA signaling (Murata et al., 2019). Application of loliolide to tomato leaves reduced the survival rate of the two-spotted spider mite and larvae of the common cutworm, without exhibiting direct toxic activity against these herbivores (Murata et al., 2019). Moreover, loliolide [also referred to as (–)-loliolide] was found to act as a soil-borne signaling molecule that enhances the production of allelochemical, such as momilactone B and tricin, to coordinate barnyardgrass-rice allelopathic interactions (Kong et al., 2018; Li et al., 2020).

Besides their biological functions within plants, many apocarotenoid volatiles are recognized as important aroma components of different flowers and fruits of horticultural plants (Shi et al., 2020). For instance, linear volatile apocarotenoids, such as geranyl acetone, 6-methyl-5-hepten-2-one (MHO), and geranial, were identified as important aroma components in fruits of papaya, citrus, and blackberry (González-Mas et al., 2011; Jing et al., 2015; Reidel et al., 2016). Cyclic apocarotenoids, such as β-CC, β-ionone, and β-damascenone, have lower odor thresholds and stronger impacts on people’s aroma perception, compared with linear volatiles (Goff and Klee, 2006). Therefore, cyclic apocarotenoids are recognized as more important volatiles with respect to the aromatic odor of fruits and flowers (Shi et al., 2020). The C₁₃ cyclic β-damascenone has an attractive honey-like, floral, and fruity flavor and shows a very low odor threshold of 2 ng/L on people’s aroma perception, hence, it has been frequently used as flavoring ingredient in fragrance industry (Liang et al., 2021). The cyclic volatiles α- and β-ionone have violet-like, woody, and fruity odor, while β-CC has a grassy, woody, tobacco-like odor. Both of them are widely used as flavor molecules in fragrances. In addition, these apocarotenoids volatiles also function as effective attractants for pollinators and seed dispersers (Liang et al., 2021).

Apart from being aroma compounds, apocarotenoids can contribute to the color of flowers and fruits and act as antioxidants. Indeed, some long-chain apocarotenoids and their derivatives are used as high-value food colorants, cosmetic agents, and antioxidants and have an important impact in promoting human health. β-citraurin (3-hydroxy-β-apo-8′-carotenal) and β-citraurinene are two major C₃₀ apocarotenoid pigments found in Citrus, and their hyper-accumulation is responsible for the attractive red coloration of fruit peel in some citrus species (Zheng et al., 2019). Bixin (C₂₅) is a further natural apocarotenoid pigment that confers red color to B. orellana seeds. It has been widely extracted from natural sources and used in food and cosmetic industry as a color additive (Giuliano et al., 2003b). Furthermore, Pacheco et al. (2019) demonstrated anti-inflammatory and antinociceptive activities of bixin, which were shown to be caused by reduction of neutrophil migration in simulated wound tests in rats. The proposed biosynthetic pathway of bixin is supposed to start with the cleavage of all-trans-lycopene into bixin aldehyde, followed by three sequential conversion steps catalyzed by the bixin aldehyde dehydrogenase and nor-bixin carboxyl methyltransferase (Bouvier et al., 2003). Saffron is one of the oldest natural food additives and worldwide the most expensive spice. It corresponds to the stigma of saffron flowers, which accumulates the 3-OH-β-cyclocitral glycoside picrocrocin, the C₁₀ volatile apocarotenoid safranal, and the C₂₀ pigment crocetin and its glycosides crocins. Crocetins and crocins are responsible for the red color, while picrocrocin and safranal give rise for the bitter taste and pungent aroma of saffron (Frusciante et al., 2014). These apocarotenoids are beneficial to health and are commonly used in food and cosmetics in many countries. Saffron apocarotenoids were also shown to have several pharmacological effects, such as anti-inflammatory, antiaging, analgesic, and neuroprotective (Bukhari et al., 2018).

The production of plant apocarotenoids varies depending on developmental stage and tissue and is affected by environmental stimuli that lead to non-enzymatic cleavage of carotenoid. Due to their role as regulatory metabolites, the genesis of several apocarotenoids is tightly regulated at different levels, including transcriptional, post-translational, and epigenetic regulation.

Many crop plants, especially horticulture crops, show large variation in quantity and types of apocarotenoids among different species and/or different natural accessions of a single species. For instance, different papaya accessions display big natural variation in accumulating MHO and β-ionone in fruits (Jing et al., 2015). Farnesyl acetone, α-ionone, and β-ionone show also high natural variation among orange-colored “Nairobi” and “Rothild,” yellow-colored “Yellowstone,” purple “Purple Haze,” and white “Creme de Lite” carrot accessions (Yahyaa et al., 2013). As direct non-enzymatic degradation is supposed to be a major source of apocarotenoids, different concentration of carotenoids may be the reason for the diversity in apocarotenoid levels; however, more and more studies reported that some genetic factors can also determine the apocarotenoid variation, independent of carotenoid levels. For example, in citrus, α-ionone, geranial, and β-ionone are common in “Temple” hybrid mandarin fruit, but cannot be detected in “Murcott” hybrid that even contains significantly higher carotenoid amounts (Yu et al., 2015), In apricot fruit, β-ionone and dihydro-β-ionone are considered as two of the major aroma compounds for fruit quality by odor activity values (OVA) method. Contents of β-ionone and dihydro-β-ionone were found to be significantly higher in pulps of white apricot cultivars, such as “Luntaixiaobaixing” and “Baixing,” than in those of the yellow “Hongyuxing” and “Danxing” cultivars that even accumulate higher carotenoid content (Xi et al., 2016). This finding suggests that the variation of apocarotenoid content in different apricot species is also genetically controlled, other than only determined by the concentration of the carotenoid precursors.

Technological advancements in high-throughput sequencing, metabolomics, and data mining have enabled the integration of various omics datasets, such as genomics, transcriptomics, and metabolomics (Figure 4). The multi-omics strategy coupled with genetic and functional analysis has facilitated exploiting the natural variation of apocarotenoid pigments and volatiles, which paves the way new discoveries in metabolism, function, and evolution evolutionary of plant apocarotenoids.

For instance, Gao et al. (2019) constructed a tomato pan-genome by using genome sequence datasets of 725 tomato accessions. Comparative analyses using this constructed tomato pan-genome identified a rare allele in cultivated tomatoes defined by promoter variation, i.e., a ~ 4-kb substitution in the promoter region, of TomLoxC gene encoding a 13-lipoxygenase. Further RNA-Seq data analysis showed that accessions containing both this rare allele captured by pan-genome and another allele present in the “Heinz 1706” genome exhibit significantly higher expression levels of TomLoxC gene than that in other accessions. Further metabolites, QTL and gene expression analysis provided strong evidence that TomLoxC variation is a genetic control for natural variation in the accumulation of nine apocarotenoid volatiles common in tomato fruits and might additionally play an important role in apocarotenoid volatiles biosynthesis. The levels of specific apocarotenoid volatiles were also significantly decreased in transgenic tomato fruits with reduced expression of TomLoxC and in two knock-out Arabidopsis mutants of AtLOX2, the closest TomLoxC homolog. These experiments further confirm that LOX is a genetic determinant for natural variation of apocarotenoid formation and also reinforce the significance of alternative apocarotenoid biosynthesis route via unspecific oxidation process, besides CCD-mediated cleavage reaction. Apocarotenoid volatiles are important quality trait for tomato fruit and flavor (Tieman et al., 2017; Gao et al., 2019). However, this TomLoxC promoter allele captured by pan-genome displays strong negative selection during domestications. This may be due to the primary focus of modern breeding on tomato yield and on resistance to stresses and long shelf-life, which did not consider aroma quality traits.

In addition to volatiles, several recent studies performed apocarotenoid pigments profiling in fruits and related natural variation in some important horticulture crops, such as Citrus and Capsicum species. Citrus fruits show peel color variation among different cultivars. Besides carotenoid content and composition, the production of C₃₀ apocarotenoid pigments, such as β-citraurin, is also critical for peel pigmentation of citrus mature fruit (Ma et al., 2013; Rodrigo et al., 2013; Zheng et al., 2015, 2019, 2021). Indeed, the content of C₃₀ apocarotenoid pigments, i.e., β-citraurin and β-citraurinene, and their ratio to total carotenoids in citrus peel showed the high correlation with the color index of red-peeled progenies in a F₁ pseudo-testcross population derived from a cross between a red-peeled Citrus reticulata and a yellow-peeled Poncirus trifoliata (Zheng et al., 2019). By using mQTL, eQTL, and BSR-seq analysis, this study showed that a 5′ cis-regulatory mutation of Citrus CCD4b (CitCCD4b) is a major genetic determinant of natural variation in the accumulation of the C₃₀ pigments β-citraurin and β-citraurinene. This study demonstrated that CitCCD4b enzyme is involved in the biosynthesis of β-citraurinene, which indicates a novel reaction in modification of aldehyde group of β-citraurin. The presence of a specific SNP in the MITE of CitCCD4b promoter is strongly correlated with the high expression level of CitCCD4b among progenies of pseudo-testcross population as well as within 115 different citrus accessions, resulting in the high-accumulation of C₃₀ apocarotenoid pigments responsible for the red coloration of citrus peel. A recent study also found that a red-peeled mutant of “Huyou” (Citrus changshanensis) contains much higher content of the C₃₀ apocarotenoid β-citraurin, accompanied by 100 times higher expression level of CitCCD4b, compared to yellow-peeled ordinary “Huyou” (Luan et al., 2020b), which also correlated with the above-mentioned putative enhancer SNP in the CitCCD4b promoter (Zheng et al., 2019; Luan et al., 2020a). Very recently, it was found that the higher transcript level of CitCCD4b in red peel of F1 hybrids also accompanied the higher content of the two C₁₀ apocarotenoid volatiles, β-CC and 3-OH-β-cyclocitral, in comparison to that in the yellow peel of three other progenies (Zheng et al., 2021). This result suggests a dual role of CitCCD4b expression variation in the natural variation of both apocarotenoid pigments and volatiles among different citrus species and in fruit pigmentation and aroma formation.

However, in other crops, such as peach and Brassica species, mutations in coding sequence of CCD4 enzyme enhanced the carotenoid pigments in tissues/organs, due to the capability of this enzyme in cleaving colorful carotenoids into C₂₇ apocarotenoids that are catabolized into colorless smaller metabolites (Falchi et al., 2013; Mi and Al-Babili, 2019). Recent Studies demonstrated that CRISPR/Cas9-mediated disruption of CCD4 gene in Ipomoea nil and carrot resulted in a remarkable enhancement of carotenoids and led to pale-yellow phenotype in petal of I. nil and yellow phenotype in the taproot of a white-colored carrot variety, respectively (Watanabe et al., 2018; Li et al., 2021). The corresponding CCD4 enzymes have also been reported to be responsible for the formation of apocarotenoid volatiles in tissues/organs, such as in peach fruit (Brandi et al., 2011). The crucial step of future research is to determine whether the natural genetic variation of such CCD4s is also linked to the diversity of apocarotenoid volatiles among different accessions, which may be utilized in molecular breeding for improvement of fruit/vegetable flavor.

Apart from apocarotenoid pigments and volatiles, apocarotenoid-derived hormones also show natural variation among different accessions. For example, at low water potential, Arabidopsis accession “Shahdara” (Sha) showed less accumulation of ABA than that in “Columbia” (Col) or “Landsberg erecta” (Ler). Genetic analysis of Ler × Sha recombinant inbred lines (RILs) and complementation experiments demonstrated that NCED3 variation is responsible for the difference in ABA accumulation between Sha and Ler accession. Further sequence analysis, site-directed mutagenesis and structural observations revealed that nonsynonymous substitutions of NCED3 coding sequence found in Sha are associated with decreased ABA accumulation and altered NCED3 post-translational processing, which may affect NCED3 activity (Kalladan et al., 2019). The rice cultivars “Bala” and “Azucena” showed large difference in SL biosynthesis that is responsible for their degree of tillering and distinct susceptibility to Striga infection (Cardoso et al., 2014). By using a “Bala” × “Azucena” F6 RIL population, Cardoso et al. (2014) identified a major QTL, a rearrangement of a 51- to 59-kbp stretch between 28.9 and 29.0 Mbp of chromosome 1, which explains most of the variation in the SL contents of rice exudates. This genomic rearrangement leads to deletion of two AtMAX1 orthologs and is associated with low SL contents in 367 rice cultivars.

Apocarotenoids are not just carotenoid degradation products but play important functions as antioxidants, pharmaceuticals, scents, and pigments, beside their role as precursors of plant hormones, signaling molecules, and growth regulators. In this review, we have briefly introduced the significance of apocarotenoid compounds for human health and their biological functions that are critical for plant development, biotic interactions, and stress response and provided an update on recently identified biosynthetic pathways and their regulation. More and more studies have shown that exogenous application of apocarotenoids is able to affect plant growth and development, as well as both abiotic and biotic stress resistance in many plants, which uncovered their biological function as regulatory metabolites. Nevertheless, the correlation between natural variation in the content of these apocarotenoids and plant phenotypes remains elusive. It is still unknown why apocarotenoids with slightly different structures have different activities and diverse functions in plants and some apocarotenoids, i.e., zaxinone, exerts contradictory effects depending on plant species. We proposed that the exploration of biochemical and genetic reasons of apocarotenoid metabolic diversity/variation could accelerate exploring currently unknown/unclear bioactive apocarotenoids pathway that could help understand their contributions to crops end-phenotypes, as well as their regulation and modifications that are still poorly understood. To date, the progress in determining natural genetic variation mechanisms and its molecular background is limited to only few apocarotenoid volatiles and pigments, while that of other important apocarotenoids, such as crocins, bixin, zaxinone, and anchorene, and apocarotenoid-derived phytohormones, is still poorly characterized. The rapid development of next-generation sequencing strategies provides reliable reference genomic sequences that are now available for most crop plants. We anticipate that mass spectrometry apocarotenoids profiling coupled with multi-omics strategies, including quantitative trait locus (QTL) mapping, genome-wide association (GWA), and RNA-seq analysis, will pave the way for uncovering new QTLs for apocarotenoid metabolite traits and identifying their underlying genes in various important crops (Figure 4). Advancement of knowledge in this area will enable to identify DNA marker-apocarotenoid traits associations for marker-assisted improvement of crops agronomic traits, such as aroma and color of fruits and flowers, related to apocarotenoid content, but also provide new metabolic engineering and/or CRISPR editing target for improvements of high-value beneficial apocarotenoids in crops in a low-cost, efficient, stable, and environment-friendly manner, to satisfy increasing demands for better crop quality in the future.

XZ organized and drafted the manuscript with substantial input from YY and designed and prepared the figures with input from YY. YY and XZ prepared the table. SA-B supervised the writing of the review and was involved in its further editing and revision. All authors contributed to the article and approved the submitted version.

This work was supported by baseline funding and Competitive Research Grants (CRG 2017 and CRG 2020) given to SA-B from King Abdullah University of Science and Technology (KAUST).

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The handling editor declared a past co-authorship with one of the authors SA-B.

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