Front. Plant Sci.Frontiers in Plant ScienceFront. Plant Sci.1664-462XFrontiers Media S.A.10.3389/fpls.2020.556312Plant ScienceReviewA Comprehensive and Comparative Analysis of the Fucoidan Compositional Data Across the PhaeophyceaePonceNora M. A.*StortzCarlos A.*Departamento de Química Orgánica, Ciudad Universitaria, Facultad de Ciencias Exactas y Naturales, Consejo Nacional de Investigaciones Científicas y Técnicas, Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR/CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina
Edited by: Cécile Hervé, Laboratoire de Biologie Intégrative des Modèles Marins, Station Biologique de Roscoff, France
Reviewed by: Anne S. Meyer, Technical University of Denmark, Denmark; Jasna Miroljub Nikolic, UMR 8227 Laboratoire de Biologie Intégrative des Modèles Marins, France
*Correspondence: Nora M. A. Ponce, aponce@qo.fcen.uba.arCarlos A. Stortz, stortz@qo.fcen.uba.ar
This article was submitted to Plant Systematics and Evolution, a section of the journal Frontiers in Plant Science
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In the current review, compositional data on fucoidans extracted from more than hundred different species were surveyed through the available literature. The analysis of crude extracts, purified extracts or carefully isolated fractions is included in tabular form, discriminating the seaweed source by its taxonomical order (and sometimes the family). This survey was able to encounter some similarities between the different species, as well as some differences. Fractions which were obtained through anion-exchange chromatography or cationic detergent precipitation showed the best separation patterns: the fractions with low charge correspond mostly to highly heterogeneous fucoidans, containing (besides fucose) other monosaccharides like xylose, galactose, mannose, rhamnose, and glucuronic acid, and contain low-sulfate/high uronic acid proportions, whereas those with higher total charge usually contain mainly fucose, accompanied with variable proportions of galactose, are highly sulfated and show almost no uronic acids. The latter fractions are usually the most biologically active. Fractions containing intermediate proportions of both polysaccharides appear at middle ionic strengths. This pattern is common for all the orders of brown seaweeds, and most differences appear from the seaweed source (habitat, season), and from the diverse extraction, purification, and analytitcal methods. The Dictyotales appear to be the most atypical order, as usually large proportions of mannose and uronic acids appear, and thus they obscure the differences between the fractions with different charge. Within the family Alariaceae (order Laminariales), the presence of sulfated galactofucans with high galactose content (almost equal to that of fucose) is especially noteworthy.
fucoidansbrown seaweedsphaeophyceaetaxonomyphylogenyPIP 298/14Secretaría de Ciencia y Técnica, Universidad de Buenos Aires10.13039/501100010253Consejo Nacional de Investigaciones Científicas y Técnicas10.13039/501100002923Agencia Nacional de Promoción Científica y Tecnológica10.13039/501100003074Introduction: Aim of the Review
Fucoidans are sulfated polysaccharides present in the cell walls of the Phaeophyceae (brown seaweeds) composed usually by fucose (Fuc) as the main monosaccharide, but accompanied by very variable amounts of other monosaccharides like galactose (Gal), xylose (Xyl), mannose (Man), rhamnose (Rha), and/or glucuronic acid (GlcA). The scientific literature on different aspects of fucoidans is steadily growing, mostly due to the diverse biological activities found for samples from many different species of seaweeds. This bioactivity (antiviral, anticoagulant, antitumoral, antioxidant, among others) has been reviewed extensively (Cosenza et al., 2017; Senthilkumar et al., 2017; Wang et al., 2019). Many studies attempted to explore the structural details of fucoidans, but it was very difficult to find a common trait in the different fucoidans so far analyzed (Bilan and Usov, 2008; Kopplin et al., 2018). This marks a big difference with red seaweed sulfated galactans, showing an unchallenged disaccharidic repeating structure modified by the position of sulfation, the series of the α-galactose units and its possible presence as a 3,6-anhydro ether (Usov, 2011). For these galactans, it has been found that the taxonomic order (or sometimes the family) to which the seaweed yielding the galactan belongs has a strong influence on the characteristics of these galactans, i.e., chemotaxonomy appears to be in effect (Miller, 1997; Stortz and Cerezo, 2000). For instance, within the brown seaweeds, it has been postulated that the fucoidans from the Laminariales tend to have just α-3-linked Fuc units, whereas those of the Fucales show more proportions of a α-(1,3)-α-(1,4) alternating structure (Deniaud-Bouët et al., 2014), as a chemotaxonomical trait related to structure. A previous review by Ale et al. (2011) has tried to establish some relationship with taxonomy, with the focus set on extraction methods, qualitative compositional data, and structural features. In this review, compositional data on fucoidans originated in different taxonomic groups of the Phaeophyceae will be presented. Two hypotheses are put into consideration: (a) that there is a relationship between some of these compositional features and the taxonomic classification, and (b) that various other factors produce the differences in composition.
Taxonomy of the Phaeophyceae
The taxonomy of brown algae (Heterokonta, Ochrophyta, Phaeophyceae) had many controversies throughout the history (Silberfeld et al., 2014). Order delineation in the Phaeophyceae has traditionally been based on the type of life cycle, reproductive aspects, mode of growth, and filamentous vs. parenchymatous construction of the thallus (Rousseau and de Reviers, 1999a, b). However, with the advent of molecular systematics, new insights were brought, thoroughly reshaping the evolutionary concepts of brown algae. Rousseau and de Reviers (1999b) and de Reviers et al. (2007) have provided a detailed evolution of classificatory concepts within the Phaeophyceae. Several changes in the classification at the ordinal level have been set between the Oltmanns (1922), comprising 8 orders to the present times classification, encompassing 18 orders (Silberfeld et al., 2014; Figure 1). Major changes were produced after the DNA sequencing of brown seaweeds started in 1993 (Draisma et al., 2003; de Reviers et al., 2007). Different molecular markers can be used, but phylogenetic studies of Phaeophyceae have mostly utilized the rDNA sequences, which include four subunits (18S, 5.8S, 26S, and 5S), containing regions which are highly conserved as well as others highly variable. Most information arose from studies on the 18S subunit of rDNA, although those studies had limited results for more recent Phaeophycean lineages (Tan and Druehl, 1996). In this way, Rousseau et al. (2001) utilized the 26S sequence, which altogether with a larger taxonomic sampling, solved some of the earlier divergences. Thus, a phylogenetic tree was constructed (Draisma et al., 2001, 2003). It has been concluded that morphological characters, many times useful to understand the ecology of brown seaweeds, have no value at all for phylogeny. Different degrees of organization, diffuse or apical growth, or life stages have appeared and disappeared repeatedly in the history of the different taxonomic groups.
Phylogenetic tree for the different orders of the Phaeophyceae (adapted from Silberfeld et al., 2014; reproduced with kind permission from the authors). One diverging branch from the order Scytothamnales containing the family Bachelotiaceae has been removed from the figure for the sake of simplicity.
Silberfeld et al. (2014) have introduced a thorough phylogenetic analysis based on a dataset generated previously (Silberfeld et al., 2011), including seven markers, for a total of 6804 nucleotides, determined for 91 Phaeophycean taxa, including minor orders for which there were very few studies. In this way, the shape of phylogenetic trees changed sharply the previous knowledge (Silberfeld et al., 2011; Charrier et al., 2012). Figure 1 depicts the outcome of the tree for the 18 orders determined by Silberfeld et al. (2014), grouped in four subclasses (Discosporangiophycidae and Ishigeophycidae, including one order each, Dictyotophycidae, including four orders, and Fucophycidae, including the remaining 12 orders).
Polysaccharides From the Phaeophyceae: The Fucoidans
Most macroalgae exhibit polysaccharides as their most abundant constituents. Taking into account their function, they can be classified into two main groups: storage and structural polysaccharides. The formers are polymers such as starch/glycogen or laminaran considered as food reserve materials, whereas the latters are structural elements of the cell walls, intercellular tissues and mucilaginous matrix. Sulfated polysaccharides are a group of anionic structural polysaccharides, useful for the seaweed in the marine environment to avoid desiccation. Their gross composition is characteristic of each algal group (galactans in red seaweeds, fucoidans in brown seaweeds, rhamnoglucuronans, and arabinogalactans in green seaweeds, van den Hoek et al., 1996), whereas more or less subtle differences appear often depending on the order, family, genus and species, as well as sometimes on the season, geographic location, or reproductive stage (Mackie and Preston, 1974). Other roles of the polysaccharides might include participations in cell-cell communication (Deniaud-Bouët et al., 2014), and in cell division processes (Skriptsova, 2015).
In macroalgae, the cell walls comprise a fibrillar skeleton immersed in an amorphous matrix. In the case of the Phaeophyceae, the fibrillar skeleton is mainly made up of cellulose [a linear β-(1→4)-glucan], and the surrounding matrix is composed predominantly by alginic acid or its salts, together with a system of sulfated polysaccharides (the fucoidans; Mackie and Preston, 1974). In this way, the cell wall is composed of two different layers: the inner layer consisting of a skeleton of microfibrils providing rigidity to the cell wall, and the outermost layer, which is usually observed as a poorly crystalline matrix in which the set of microfibrils is embedded. There is also evidence that the matrix does not penetrate the fibers, but remains attached to this layer through hydrogen bonds (Davis et al., 2003). It has been suggested that fucoidans might play a key role in cell wall architecture, cross-linking cellulose and alginates (Kloareg et al., 1986). Besides this function, as occurs with other sulfated polysaccharides, the fucoidans help to protect the plant from desiccation. When the fronds are in contact with sea water the sulfate hemiester groups are strongly associated with magnesium ions, which are highly hydrated and thus retain water in the fronds (Percival, 1979). In a more modern model for the Fucales (Deniaud-Bouët et al., 2014, 2017; Torode et al., 2016), it has been proposed that two networks are assembled in the cell wall; the first one contains the fucoidans interlocking a cellulose (or other β-glucans) network, and the second one contains alginate crosslinked by polyphenols. The rigidity is controlled by the alginate structure and its calcium cross-linking capabilities, whereas the fucoidans participate mostly in adaptation to the osmotic stress.
More than one century ago, Kylin has isolated for the first time (from different seaweed species of the genera Fucus, Laminaria, and Ascophyllum) a group of sulfated polysaccharides with a high Fuc content and called them “fucoidin” (Kylin, 1913). Originally the name fucoidin (later changed to the more systematic fucoidan) was coined for the polysaccharides from those species, but this term was rapidly extended to any fucose-rich polysaccharides, including not only those becoming from brown seaweeds, but also to those present in echinoderms (Olatunji, 2020). As noted above, fucoidans are sulfated polysaccharides present mainly in the intercellular tissue of mucilaginous matrix of the cell walls of brown algae (Deniaud-Bouët et al., 2017).
Fucoidans comprise a family of diverse molecules containing, in addition to Fuc, varying proportions of Gal, Man, Xyl and GlcA (Figure 2). Acetate esters have also been found, especially in modern studies (see below). In the early studies extensive purification was carried out in an effort to isolate a “fucan” containing only Fuc residues, assuming that the remaining monosaccharides were originated in other, contaminating polysaccharides. Nevertheless, even in the allegedly pure samples, small proportions of Gal, Xyl, and/or uronic acid persisted (Percival, 1979). Later, only in a few species a pure fucan was isolated after purification (see below). Thus, most of the samples so far isolated are heterofucans (Deniaud-Bouët et al., 2014).
Main structural monosaccharidic units of fucoidans. These monosaccharides can appear as terminal non-reducing units or linked through any of the free hydroxyl groups. Usually Fuc and GlcA appear linked through O-3 or O-4, Xyl through O-4, Gal through O-3 or O-6 and Man through O-2 (Sakai et al., 2003; Bilan et al., 2010, 2017, 2018). The structural features of Rha are unknown. For representative structures of fucoidans (see Deniaud-Bouët et al., 2017).
Fucoidans From Different Species of Phaeophyceae
In this section, the main chemical characteristics of fucoidans extracted from different species of brown seaweeds reported so far to the best of our knowledge (with compositional data provided) will be described in tabular form. They will be shown separately for each of the different orders (Figure 1). When numerous species of an order were studied, separations in families or genera are also displayed. It is worth noting that depending on the way that the analyses were expressed in the original papers, the uronic acids in the following tables were indicated as a percentage of the total sample (in most cases) or as part of the molar ratio of all the monosaccharides. Thus, these molar ratios might or might not include the uronic acid components. The main monosaccharidic units appearing in fucoidans are shown in Figure 2. When the authors have isolated a large number of fractions, only those more abundant or representative are listed in the tables. The reported presence of acetyl groups is indicated qualitatively with the “Ac” acronym. It should be noted that the geographic location and season of harvest of the seaweed can also have significant effects on the composition of the extracted fucoidans (e.g., Zvyagintseva et al., 2003). The extraction and fractionation procedures are schematically displayed, neglecting defatting and depigmenting steps, as well as usual procedures like dialysis or single alcohol precipitations. The methods used for monosaccharide and sulfate quantitation are also shown.
Fucales
As expected, samples of fucoidans from this is order were the most studied. Samples from five different families of the Fucales have been studied. Two species from the Fucaceae, i.e., Fucus vesiculosus and Ascophyllum nodosum appear in the earlier studies by Kylin (1913). The polysaccharides from these species were studied extensively by different research groups (see below). However, the family with more species studied was the Sargassaceae. Considering only the genus Sargassum, studies on the fucoidans from 26 different species were found in the current survey.
The extraction of fucoidans from Fucus vesiculosus was originated in the early Kylin studies, when Fuc was characterized after hydrolysis as phenyl-L-fucosazone; pentoses in the hydrolyzate were also reported (Kylin, 1913). Different products from this species were extensively studied (Table 1). Originally, the presence of Xyl was ascribed to a contaminating xylan that accompanied the fucoidan (Percival and McDowell, 1967). As a matter of fact, they reported the isolation of a xylan, although uronic acid residues were found in the xylan fraction and, furthermore, the authors were not able to separate any fraction composed just by Fuc residues. The studies by Nishino et al. (1994a) on a commercial sample from this seaweed were highly comprehensive: they were able to separate 13 different fractions and analyze them thoroughly, showing structures ranging from typical fucans (containing mainly Fuc and sulfate, and free of uronic acids) to heteropolysaccharides with low sulfate content and high content of uronic acids. In a minor fraction, they were able to find an appreciable amount of glucosamine (11.5%). In an interesting study using microwave extraction of this seaweed, Rodríguez-Jasso et al. (2011) showed that depending on the pressure and extraction time, fucoidans with different ratios Fuc/Gal were obtained (ranging from 100% Fuc to a 1:1 ratio), plus variable proportions of Xyl and sulfation degrees. Another species from the same genus that has been studied is Fucus evanescens. Zvyagintseva et al. (1999) separated the polysaccharides using a chromatography system on a hydrophobic resin. It is interesting to note that in a subsequent work Zvyagintseva et al. (2003) analyzed specimens of three different seaweeds (F. evanescens, Laminaria cichorioides, and Saccharina japonica) collected at different places, at various stages of development and at different seasons, and found some notable differences, particularly for the F. evanescens equivalent fractions obtained in different geographic locations (ratio Fuc/sulfate between 1 and 2.1; Fuc proportion from 56 to 80%; molecular masses from 14–40 to 150–500 kDa).
Reported compositions of the fucoidans from the family Fucaceae (Fucales).
Species
Extraction
Purification/
Acronym
Monosaccharide composition (moles %)
Sulfate
UA (%)
References
Fractionationa
Methodb
Fuc
Xyl
Gal
Man
Glc
Rha
GlcA
Others
Methodc
%
Fucus vesiculosus
HCl pH 2
Ethanol ppt
F1
GC
50
15
4
17
14
Pb
4
22
Medcalf and Larsen (1977a)
HCl pH 2
Ethanol ppt
F2
GC
70
7
8
4
11
Pb
25
6
“
HCl 0.01M+CaCl2 1%
GC
79
10
6
3
2
Tit
31
14
Mabeau and Kloareg (1987)
pH 7.5+CaCl2 1%
EtOH+TCA 10%
FF
GC
84
2
13
1
Tit
26
4
Mabeau et al. (1990)
Triton 0.5%, pH 7.5+CaCl2 1%
EtOH+TCA 10%
TF
GC
60
10
14
10
6
Tit
14
9
“
HCl 0.01M+CaCl2 1%
HT
GC
87
4
5
2
2
Tit
39
17
“
Na2CO3 3%
HCl 0.01M ppt
OHF
GC
78
11
5
3
3
Tit
30
9
“
SigmaTM
GC
92
4
3
2
DP
23
8
Nishino et al. (1994a)
SigmaTM
SEC+AEC
I1.8
GC
90
3
5
2
DP
32
3
“
SigmaTM
SEC+AEC
II1.35
GC
94
1
5
tr.
DP
33
–
“
SigmaTM
SEC+AEC
II2
GC
94
1
5
DP
36
–
“
SigmaTM
SEC+AEC
III11.5
GC
93
2
5
DP
34
–
“
H2O, r.t.
F1
GC
55
11
9
25
DP
6
39
Rupérez et al. (2002)
HCl 0.1M
F3
GC
89
6
5
DP
11
9
“
CaCl2 2% hot
PQA
GC
67
6
13
8
6
DP
24
10
Cumashi et al. (2007)
CaCl2 2% hot
GC
59
13
10
3
14
EA
18
7
Bittkau et al. (2020)
CaCl2 2% hot
PQA
HPLC
83
6
7
3
1
DP
25
1
Zhang et al. (2015)
Fucus ceranoides
HCl 0.01M+CaCl2 1%
GC
80
10
7
4
Tit
31
12
Mabeau and Kloareg (1987)
Fucus distichus
CaCl2 2% hot
PQA + AEC
F1
GC
84
10
3
2
1
DP
24
–
Bilan et al. (2004)
CaCl2 2% hot
PQA + AEC
F3
GC
83
9
4
2
1
DP
24
–
“
CaCl2 2% hot
PQA + AEC
F4
GC
96
2
2
Ac
DP
35
–
“
Fucus evanescens
HCl 0.4% r.t.
HC
F-1
HPLC
90
3
1
6
DP
∼12
ND
Zvyagintseva et al. (1999)
HCl 0.4% r.t +H2O hot
HC
F-2
HPLC
91
7
1
DP
∼25
ND
“
CaCl2 2% hot
PQA + AEC
F3
GC
67
16
9
7
DP
29
11
Bilan et al. (2002)
CaCl2 2% hot
PQA + AEC
F4
GC
94
3
3
Ac
DP
46
–
“
HCl pH 2-2.3 hot
AEC
FeF
HPLC
87
2
2
4
1
DP
28
ND
Anastyuk et al. (2012b)
HCl 0.2M hot
Sterile
HPLC
69
7
9
8
6
1
ND
ND
Skriptsova et al. (2012)
HCl 0.2M hot
Reprod.
HPLC
77
5
5
3
10
ND
ND
“
HCl pH2-2.3
FeF
HPLC
78
8
10
4
Ac
DP
23
ND
Prokofjeva et al. (2013)
CaCl2 2% hot
GC
96
4
EA
27
4
Bittkau et al. (2020)
d
Enz.pH6 + CaCl2 2%
AEC
FeF2
PAD
75
3
15
2
1
1
HexA 3
DP
35
e
Nguyen et al. (2020)
d
Enz.pH6 + CaCl2 2%
AEC
FeF3
PAD
88
2
9
HexA 1
DP
39
e
“
Fucus serratus
HCl 0.01M+CaCl2 1%
GC
76
18
5
1
Tit
22
15
Mabeau and Kloareg (1987)
CaCl2 2% hot
AEC
F3
GC
86
6
4
2
1
Ac
DP
22
–
Bilan et al. (2006)
CaCl2 2% hot
AEC
F4
GC
94
3
3
Ac
DP
32
–
“
CaCl2 2% hot
PQA + AEC
GC
69
7
13
6
5
DP
29
8
Cumashi et al. (2007)
CaCl2 2% hot
GC
41
10
4
2
43
EA
12
6
Bittkau et al. (2020)
Fucus spiralis
HCl 0.01M+CaCl2 1%
GC
90
7
3
tr.
Tit
36
10
Mabeau and Kloareg (1987)
CaCl2 2% hot
PQA
GC
80
7
7
3
3
DP
26
8
Cumashi et al. (2007)
Ascophyllum nodosum
HCl 0.2M
AP/R
Ascoph.
CC
49
51
BC
12
19
Larsen et al. (1966)
HCl 0.2M +AP/R
CaCl2 0.04M+CE
F2
CC
86
14
BC
30
3
“
H2O + OA pH 2.8f
CaCl2 2%
GC
70
14
16
JL
21
11
Percival (1968)
HCl pH 2
Ethanol ppt
F1
GC
37
29
3
21
11
M
13
26
Medcalf and Larsen (1977a)
HCl pH 2
Ethanol ppt
F2
GC
73
11
2
10
5
M
21
16
“
HCl pH 2
Ethanol ppt
F3
GC
81
9
2
4
4
M
25
6
“
HCl pH 2
Ethanol ppt
F4
GC
34
14
27
15
10
M
15
7
“
HCl pH 2
Ethanol ppt
F5
GC
71
7
14
4
4
M
8
7
“
HCl pH 2
CaCl2 1M+AP/R
GC
44
4
40
4
HexA 8
M
15
8
Medcalf et al. (1978)
CaCl2 2% hot
PQA
GC
67
11
12
7
3
DP
24
9
Cumashi et al. (2007)
H2O + HCl 0.2M
AP/R
HPLC
47
40
2
10
1
DP
10
21
Nakayasu et al. (2009)
H2O + HCl 0.2M
AP/R
HPLC
82
8
7
2
1
DP
24
2
Zhang et al. (2015)
HCl 0.1M, MWg
CaCl2 2%
PAD
40
14
6
11
24
DP
27
e
Yuan and Macquarrie (2015)
Ascophyllum mackaii
H2O hot
CaCl2 1%+AP/R
AMF
HPLC
57
4
16
9
2
2
11
DP
22
e
Qu et al. (2014)
Pelvetia canaliculata
pH 7.5+CaCl2 1%
EtOH+TCA 10%
FF
GC
82
4
10
2
2
Tit
29
4
Mabeau et al. (1990)
Triton 0.5%, pH 7.5+CaCl2 1%
EtOH+TCA 10%
TF
GC
65
13
11
6
5
Tit
20
6
“
HCl 0.01M+CaCl2 1%
HT
GC
81
9
7
2
1
Tit
40
2
“
Na2CO3 3%
HCl 0.01M ppt
OHT
GC
90
4
4
1
1
Tit
33
4
“
Silvetia babingtonii
HCl pH 2-2.3 hot
AEC
SbF
HPLC
77
5
12
6
DP
25
ND
Anastyuk et al. (2012b)
HCl 0.2M hot
Sterile
HPLC
71
7
6
5
10
ND
ND
Skriptsova et al. (2012)
HCl 0.2M hot
Reprod.
HPLC
80
6
6
4
4
ND
ND
“
aKey: AEC, anion exchange chromatography; SEC, size-exclusion chromatography; HC, hydrophobic chromatography; CE, cation exchange; PQA, precipitation with quaternary ammonium salts; AP/R alcohol precipitation and redissolution.bKey for the less common abbreviations: PAD, HPAEC with pulse amperometric detector; GC, gas chromatography; CC, column chromatography on carbon-Celite.cKey: DP, method of Dodgson and Price (1962) or equivalent; Pb, titration with lead nitrate (Medcalf et al., 1972); EA, elemental analysis; Tit, titration with cetylpyridinium chloride, pH 1.5 (Scott, 1960); BC, method of barium chloranilate (Lloyd, 1959).dAnalyzed as Fucus distichus subsp. evanescens.eThe information for the uronic acid is included in the molar ratio of monosaccharides.fOxalic acid/ammonium oxalate extraction of the residue.gMicrowave-aided extraction.
It should be mentioned that the high proportions of Glc found in some unpurified extracts are probably becoming from laminaran. This has occurred, for instance, in the sample of Fucus serratus isolated by Bittkau et al. (2020), as lower proportions of this monosaccharide have been found in other studies (Table 1). The studies of Bilan et al. (2002, 2004, 2006) on different Fucus species, carried out with careful separations involving anion exchange chromatography have shown in all cases that at high ionic strengths, they were able to isolate, with good yields, a fucan sulfate almost devoid of other monosaccharides (Fuc ≥ 94%, Table 1, fraction F4).
Ascophyllum nodosum is the other characteristic species from the family Fucaceae which has been thoroughly studied since the early studies of Kylin (1913), followed by further reports indicating the presence of a sulfated polysaccharide with a Fuc/Gal ratio of 8:1 (Percival and McDowell, 1967). The name ascophyllan was coined (to distinguish from the fucoidan characteristic of Fucus vesiculosus) for the isolated polysaccharide, composed of Fuc, Xyl, and sulfate groups, along with uronic acids. Medcalf and Larsen (1977a, b) determined a complex mixture of polysaccharides in this seaweed, and concluded that the fucan constituted the backbone of the molecule, whereas the ascophyllan-like components were attached as branches. Besides, they also determined that the uronic acid present was not glucuronic acid, as indicated in previous reports, but mannuronic and guluronic acid, i.e., the components of alginic acid, suggesting that contamination with this polysaccharide was difficult to avoid. For the fucoidans of this seaweed, an attempt was made to compare the results of the various researchers (Table 1), taking into account that most extractions were carried out in acid medium. However, the original Fuc/Xyl ratio close to 1 found by Larsen et al. (1966) was only reproduced by Nakayasu et al. (2009). Medcalf and Larsen (1977a) found a series of highly heterogeneous fractions, whereas 1 year later, using the same seaweed sample, Medcalf et al. (1978) found a polysaccharide with a Fuc/Gal ratio close to 1. The proportion of uronic acids in purified samples varied between 2 and 21%, whereas the content of sulfate varied between 8 and 24%. In summary, no common pattern between the determinations carried out by different researchers was observed.
Within the Fucaceae, it is clear that polysaccharides from the genus Fucus tend to be fucose-rich (more than 70% of the monosaccharides), although reports diverge, and important proportions of other monosaccharides appear in some cases (Table 1). On the other hand, in the genus Ascophyllum, important proportions of Xyl and uronic acid-containing fractions appear, although some purification steps allowed to obtained fucans equivalent to those of Fucus, suggesting that mixtures of different kinds of polymers appear in all the samples that have been surveyed in this study, and they might change their proportions in the different species, and using different extraction and purification methods.
The family Sargassaceae comprises much more species than the Fucaceae (512 against 18, Guiry and Guiry, 2020). This family has the largest number of species studied from the point of view of its polysaccharides. The fucoidans from at least 26 different species of the genus Sargassum alone were analyzed. Table 2 shows the results for the different fucoidans isolated from this genus. For S. horneri, Ermakova et al. (2011) postulated the presence of Rha in substantial amounts within the polysaccharides (Table 2). However, their NMR spectra did not show the presence of this sugar, and in a further work by the same group (Silchenko et al., 2017) the fucoidans were purified without any trace of Rha. In S. latifolium, Asker et al. (2007) isolated three fractions where Glc and GlcA are the major components and Fuc is a minor one, not responding to the classical fucoidan composition. Other atypical polysaccharides were reported in S. pallidum (Liu et al., 2016) carrying high-mannose fucoidans, rich in uronic acids and scarcely sulfated, and in S. thunbergii (Luo et al., 2019), where a fucoidan completely devoid of sulfate groups was reported (Table 2).
Reported compositions of the fucoidans from the genus Sargassum (Sargassaceae, Fucales).
Species
Extraction
Purification/
Acronym
Monosaccharide composition (moles %)
Sulfate
UA (%)
References
Fractionationa
Methodb
Fuc
Xyl
Gal
Man
Glc
Rha
GlcA
Others
Methodc
%
Sargassum aquifolium
H2O + HCl pH 1
AEC
0.5M
GC
14
15
37
13
21
DP
6
28
Bilan et al. (2017)
H2O + HCl pH 1
AEC
1M
GC
41
15
29
9
6
DP
22
14
“
H2O + HCl pH 1
AEC
1.5M
GC
36
9
48
4
3
DP
29
5
“
Sargassum binderi
CaCl2 2% hot
PQA
Fsar
GC
60
5
19
7
7
Ac
EA
8
d
Lim et al. (2016)
Sargassum cinereum
H2O+CaCl2 1%
HPLC
66
7
24
3
DP
4
ND
Somasundaram et al. (2016)
Sargassum crassifolium
CaCl2 2% hot
PQA
Fsc
GC
56
2
41
1
DP
28
8
Yuguchi et al. (2016)
H2O, PTe
AP/R
SC3
PAD
37
5
37
11
11
IC
22
24
Yang et al. (2017)
Sargassum duplicatum
HCl 0.1M hot
AEC+HC
SdF1
GC
40
57
3
Ac
DP
32
ND
Shevchenko et al. (2017)
HCl 0.1M hot
AEC+HC
SdF2
GC
59
2
39
Ac
DP
38
ND
“
HCl 0.1M hot
AEC, NH3
SdF
GC
51
49
Ac
DP
32
ND
Usoltseva et al. (2017a)
Sargassum feldmanii
HCl 0.1M hot
AEC+HC
SfF2
GC
72
28
DP
25
ND
Shevchenko et al. (2017)
Sargassum filipendula
Enz.pH 8
Acetone ppt
SF-0.7
HPLC
22
16
27
16
16
DP
11
d
Costa et al. (2011)
Enz.pH 8
Acetone ppt
SF-2.0
HPLC
22
4
49
13
11
DP
18
“
Sargassum fulvellum
HCl pH 2 hot
PQA
Fr 0.5
GC
38
23
26
6
7
DP
13
23
Koo et al. (2001)
HCl pH 2 hot
PQA
Fr 3
GC
44
6
43
3
4
DP
55
4
“
Sargassum fusiforme
H2O, hot
AEC+SEC
SFPS
GC
53
9
20
21
DP
11
6
Chen et al. (2012)
Enzymes
AP/R+SEC
65A
GC
42
15
21
6
2
14
DP
17
d
Hu et al. (2016)
H2O+CaCl2 2%
AEC+SEC
FP08S2
GC
37
18
19
7
19
EA
21
d
Cong et al. (2016)
HCl 0.01M+CaCl2 4M
AEC+SEC
SFF42
HPLC
31
6
19
29
3
12
DP
17
12
Wu et al. (2019)
HCl 0.01M+CaCl2 4M
AEC+SEC
SFF5
HPLC
50
3
31
10
3
3
DP
24
10
“
Sargassum hemiphyllum
H2O, PTe
CaCl2 2%+AP/R
SH3
PAD
54
1
19
15
3
8
Ac
IC
24
6
Huang et al. (2017)
Sargassum henslowianum
H2O, AP/R
AEC+SEC
SHAP-1
HPLC
76
24
EA
32
0
Sun et al. (2020)
H2O, AP/R
AEC+SEC
SHAP-2
HPLC
75
25
EA
32
0
“
Sargassum horneri
HCl 0.1M hot
AEC
Sh-F1
HPLC
81
3
8
7
DP
15
ND
Ermakova et al. (2011)
HCl 0.1M hot
AEC
Sh-F2
HPLC
90
10
DP
0
ND
“
HCl 0.1M hot
AEC
Sh-F3
HPLC
69
31
DP
17
ND
“
CaCl2 2% hot
AEC
GC
90
10
DP
23
ND
Silchenko et al. (2017)
Sargassum latifolium
H2O, hot
AEC+SEC
SP-I
HPLC
14
14
42
23
16
d
Asker et al. (2007)
H2O, hot
AEC+SEC
SP-II
HPLC
10
13
41
29
19
d
“
H2O, hot
AEC+SEC
SP-III
HPLC
16
12
32
35
22
d
“
Sargassum mcclurei
HCl pH 2.5 hot
HC+AEC
SmF1
HPLC
27
6
20
34
13
DP
17
ND
Thinh et al. (2013)
HCl pH 2.5 hot
HC+AEC
SmF2
HPLC
45
5
34
5
10
DP
26
ND
“
HCl pH 2.5 hot
HC+AEC
SmF3
HPLC
59
41
DP
35
ND
“
Sargassum muticum
pH 7.5+CaCl2 1%
EtOH+TCA 10%
FF
GC
44
5
46
3
3
Tit
12
9
Mabeau et al. (1990)
Triton 0.5%, pH 7.5+CaCl2 1%
EtOH+TCA 10%
TF
GC
84
2
14
Tit
8
11
“
HCl 0.01M+CaCl2 1%
HF
GC
46
21
11
17
5
Tit
9
25
“
HCl 0.1M hot
AEC
1SmF1
GC
52
33
15
DP
26
ND
Usoltseva et al. (2017b)
HCl 0.1M hot
AEC
1SmF3
GC
67
33
Ac
DP
48
ND
“
Sargassum oligocystum
HCl 0.1M hot
AEC
1SoF1
HPLC
43
4
8
35
8
DP
17
ND
Men’shova et al. (2013)
HCl 0.1M hot
AEC
1SoF2
HPLC
53
5
21
10
10
DP
24
ND
“
HCl 0.1M hot
AEC
1SoF3
HPLC
77
23
DP
32
ND
“
Sargassum pallidum
HCl 0.2M hot
Sterile
HPLC
46
8
10
10
14
13
ND
ND
Skriptsova et al. (2012)
HCl 0.2M hot
Reprod.
HPLC
52
6
16
9
3
14
ND
ND
“
H2O, r.t.
Ethanol ppt
SPC60
GC
41
5
17
27
10
DP
4
33
Liu et al. (2016)
H2O, hot
Ethanol ppt
SPH60
GC
32
4
14
23
25
DP
4
29
“
H2O, hot
Ethanol ppt
SPH70
GC
37
4
24
22
10
DP
7
20
“
Sargassum polycystum
HCl pH 2-3 hot
HC+AEC
F1
GC
29
22
19
19
11
DP
7
23
Bilan et al. (2013)
HCl pH 2-3 hot
HC+AEC
F2
GC
44
13
28
9
5
DP
20
11
“
HCl pH 2-3 hot
HC+AEC
F3
GC
69
4
25
tr.
tr.
DP
33
2
“
HCl pH 2-3 hot
HC+AEC
F4
GC
63
3
34
DP
34
2
“
Enzymes pH 4.5
CaCl2 5M
SPF
PAD
63
6
8
NIf 22
DP
28
Fernando et al. (2018)
Sargassum ringgoldianum
HCl 0.05M
Ca(AcO)2+AEC
Fr-B
GC
44
17
18
17
5
DP
16
10
Mori and Nisizawa (1982)
HCl 0.05M
Ca(AcO)2+AEC
Fr-C
GC
58
6
28
7
1
DP
24
7
“
Sargassum stenophyllum
H2O+CaCl2 4M
PQA
F2
GC
60
9
21
10
DP
19
11
Duarte et al. (2001)
H2O+CaCl2 4M
PQA
F3
GC
52
7
23
17
DP
21
10
“
H2O+CaCl2 4M
PQA
F5
GC
60
5
31
2
2
DP
28
2
“
Sargassum swartzii
HCl 0.1M +CaCl2 2%
PQA+AEC
F2
PAD
50
3
29
5
3
Ara 7
DP
15
13
Ly et al. (2005)
HCl 0.1M +CaCl2 2%
PQA+AEC
F3
PAD
56
2
29
3
3
Ara 5
DP
18
5
“
HCl 0.1M +CaCl2 2%
PQA+AEC
F4
PAD
56
2
28
4
3
Ara 4
DP
28
8
“
HCl 0.05 M+CaCl2 4%
AEC
FF1
HPLC
58
6
22
14
DP
19
18
Dinesh et al. (2016)
HCl 0.05 M+CaCl2 4%
AEC
FF2
HPLC
63
4
18
15
DP
24
13
“
Sargassum tenerrimum
HCl 0.1M +K2CO3 2%
CaCl2 2%+ HCl 0.1M
C
GC
73
15
9
3
DP/IR
2
9
Sinha et al. (2010)
Sargassum trichophyllum
H2O, hot
AEC+SEC
ST-F
GC
80
20
Rho
23
1
Lee et al. (2011)
Sargassum thunbergii
H2O+NaOH 0.5M
AEC
STSP-I
GC
55
45
DP
0
ND
Luo et al. (2019)
Sargassum vachellianum
H2O
CaCl2
SPS
HPLC
65
5
12
15
3
DP
12
1
Jesumani et al. (2020)
Sargassum vulgare
Enz. pH 8
AEC
Flo 1.5
Col.
50g
25
HexA 25
TB
∼ 15
d
Dietrich et al. (1995)
Enz. pH 8
AEC
Flo 2.5
Col.
77g
8
HexA 15
TB
∼ 41
d
“
aKey: AEC, anion exchange chromatography; SEC, size-exclusion chromatography; HC, hydrophobic chromatography; PQA, precipitation with quaternary ammonium salts; AP/R alcohol precipitation and redissolution.bKey for the less common abbreviations: PAD, HPAEC with pulse amperometric detector; GC, gas chromatography; Col., colorimetric methods.cKey: DP, method of Dodgson and Price (1962) or equivalent; IC, ion chromatography; EA, elemental analysis; IR, estimation by area of IR bands; TB, toluidine blue; Rho, rhodizonate; Tit, titration with cetylpyridinium chloride, pH 1.5 (Scott, 1960).dThe information for the uronic acid is included in the molar ratio of monosaccharides. ePT = high pressure and temperature.fNI = sugar not identified.gFuc, Xyl and uronic acid were the only monosaccharides which could be determined.
Dietrich et al. (1995) studied the polysaccharides from Sargassum vulgare, differentiating whole plants and floaters. The fucoidan fractions corresponded to sulfated xylofucans containing important proportions of uronic acids. The proportion of sulfate is clearly higher in floaters. The ratio Fuc/Xyl/HexA varied between 1:0.5:0.5 and 1:0.1:0.2. However, only Fuc, Xyl and uronic acid have been determined in this investigation, missing other sugars possibly present.
For Sargassum fusiforme, the presence of galacturonic acid was detected (Hu et al., 2014). However, it has been shown later that this monosaccharide was part of a contaminating polysaccharide which could be separated by careful fractionation (Cong et al., 2016; Hu et al., 2016).
For the remaining members of the Fucales, the data is shown in Table 3. Mian and Percival (1973) carried out studies on Bifurcaria bifurcata and Himanthalia lorea. The data is shown only partially in Table 3, as Gal could not be quantified. Fractionation by ion exchange chromatography showed fractions with high uronic acid/low sulfate content using lower ionic strengths, and high sulfate, high Fuc, low uronic acid content in the later elutions. This behavior was observed for many further studies, regardless of the taxonomy of the seaweed. In some cases, like for Nizamuddinia zanardinii, the authors have devoted a lot of work in order to search for different extraction methods (Alboofetileh et al., 2019a,b,c). In Table 3 we have included the analysis of one extraction method, as the characteristics of the polysaccharides appear to be quite similar.
Reported compositions of the fucoidans from the order Fucales not belonging to the family Fucaceae or to the genus Sargassum (Sargassaceae).
Species
Extraction
Purification/
Acronym
Monosaccharide composition (moles %)
Sulfate
UA (%)
References
Fractionationa
Methodb
Fuc
Xyl
Gal
Man
Glc
Rha
GlcA
Others
Methodc
%
Family Sargassaceae
Bifurcaria bifurcata
CaCl2 2% +HCl pH2
AEC
0.3M
GC+PC
XX
X
tre
JL
5
20
Mian and Percival (1973)
CaCl2 2% +HCl pH2
AEC
1M
GC+PC
XX
tr.
Xe
JL
30
3
“
HCl 0.01M+CaCl2 1%
GC
73
10
10
4
3
Tit
20
16
Mabeau and Kloareg (1987)
Coccophora langsdorfii
HCl 0.1M r.t.
AEC
Cf2
HPLC
86
3
7
HexA 4,Ac
DP
25
d
Imbs et al. (2016)
Cystoseira barbata
HCl 0.1M hot
CBSP
GC
45
4
34
3
8
6
Ac
EA
23
7
Sellimi et al. (2014)
Cystoseira compressa
HCl 0.1M hot
CCF
GC
62
4
24
8
DP
15
9
Hentati et al. (2018)
Cystoseira indica
H2O, r.t.
CiWE
GC
75
14
11
DP/IR
8
4
Mandal et al. (2007)
H2O, r.t.
AEC
CiF3
GC
84
7
5
4
DP/IR
9
2
“
Hizikia fusiforme
H2O+CaCl2 3M
AEC
F2
GC
38
8
18
30
4
1
DP
12
29
Li et al. (2006)
H2O+CaCl2 3M
AEC+SEC
F33
GC
38
5
22
27
5
2
DP
3
32
“
H2O+CaCl2 3M
AEC
YF5
HPLC
44
21
18
16
DP
20
d
Wang et al. (2012)
Hormophysa cuneiformis
H2O+HCl pH 1
FHC
GC
39
5
47
5
4
DP
23
5
Bilan et al. (2018)
H2O+HCl pH 1
AEC
F2
GC
33
11
50
4
2
DP
18
7
“
H2O+HCl pH 1
AEC
F3
GC
79
2
19
DP
35
2
“
Nizamuddinia zanardinii
H2O
CaCl2 1%
HWE-F
GC
31
6
28
32
5
DP
18
1
Alboofetileh et al. (2019a)
Turbinaria conoides
HCl 0.1M
AEC
AF3
GC
54
18
28
+
DP/IR
4
ND
Chattopadhyay et al. (2010)
Turbinaria ornata
HCl 0.1M hot
AEC
ToF2
HPLC
83
17
DP
32
ND
Ermakova et al. (2016)
Enzymes pH 4.5
CaCl2+AEC
F2
PAD
46
22
NIf 32
DP
10
ND
Jayawardena et al. (2019)
Enzymes pH 4.5
CaCl2+AEC
F7
PAD
63
5
6
NI 25
DP
30
ND
“
Turbinaria turbinata
Enzymes pH 5
AEC
TtF3
GC
61
2
19
4
13
Ara 1,Ac
ND
ND
Monsur et al. (2017)
Family Durvillaeaceae
Durvillaea antarctica
H2O, MWg
DAP
GC
3
3
9
78
Sorbose 8
ND
ND
He et al. (2016)
Durvillaea potatorum
HCl pH 1 hot
Acetone ppt
AFS
HPLC
32
4
64
DP
13
–
Lorbeer et al. (2017)
Family Himanthaliaceae
Himanthalia elongata
H2O+HCl 0.1M
F-HCl
GC
17
1
29
3
50
DP
6
3
Mateos-Aparicio et al. (2018)
Himanthalia lorea
CaCl2 2% +HCl pH2
AEC
0.3M
GC+PC
XX
X
tr.e
JL
2
19
Mian and Percival (1973)
CaCl2 2% +HCl pH2
AEC
1M
GC+PC
XX
tr.
Xe
JL
29
4
“
Family Seirococcaceae
Marginariella boryana
H2SO4 1% r.t.
Reprod.
GC
72
2
17
1
7
ND
3
Wozniak et al. (2015)
H2SO4 1% r.t.
Vegetat.
GC
45
21
12
13
7
2
ND
13
“
Seirococcus axillaris
HCl pH 1 hot
Acetone ppt
AFS
HPLC
61
16
14
3
2
4
DP
20
d
Lorbeer et al. (2017)
aKey: AEC, anion exchange chromatography; SEC, size-exclusion chromatography.bKey for the less common abbreviations: PC, paper chromatography; GC, gas chromatography; PAD, HPAEC with pulse amperometric detector.cKey DP, method of Dodgson and Price (1962) or equivalent; JL, method of Jones and Letham (1954); IR, estimation by area of IR bands; EA, elemental analysis by different methods; Tit, titration with cetylpyridinium chloride, pH 1.5 (Scott, 1960).dThe information for the uronic acid is included in the molar ratio of monosaccharides.eAs galactose could not be quantified, the data is semiquantitative.fNI = sugar not identified.gMicrowave-aided extraction.
For Marginariella boryana, Wozniak et al. (2015) analyzed the polysaccharides extracted from vegetative structures (blades and vesicles) and receptacles (reproductive structures) separately. The proportions of Xyl, Man, and uronic acid increase significantly in the vegetative structures (Table 3). Within the family Durvillaeaceae two species were studies. Both in Durvillaea antarctica (He et al., 2016) and D. potatorum (Lorbeer et al., 2017), the proportion of Glc was so large that it obscured the analysis of the fucoidan constituents, even when purification procedures (successful with other seaweeds) to avoid contamination with laminaran were carried out (Lorbeer et al., 2017).
Most of the fucoidans analyzed from the Fucales were galactofucans, usually with small proportions of Xyl, with the exception of those of Ascophyllum nodosum (Table 1). Man and GlcA appeared in variable amounts.
Dictyotales
The data on the fucoidans from different species of the order Dictyotales is shown in Table 4. It should be mentioned that for Dictyota mertensii, the information is incomplete, as only Fuc, Xyl and uronic acid have been determined (Dietrich et al., 1995).
Reported compositions of the fucoidans from the order Dictyotales.
Species
Extraction
Purification/
Acronym
Monosaccharide composition (moles %)
Sulfate
UA (%)
References
Fractionationa
Methodb
Fuc
Xyl
Gal
Man
Glc
Rha
GlcA
Others
Methodc
%
Canistrocarpus cervicornis
Enz.pH 8
Acetone ppt
CC-0.7
HPLC
33
17
50
DP
19
d
Camara et al. (2011)
Enz.pH 8
Acetone ppt
CC-2.0
HPLC
20
10
40
10
20
DP
20
d
“
Dictyopteris plagiogramma
CaCl2 2% +HCl pH2
C
GC
42
10
16
8
3
21
JL
4
d
Percival et al. (1981)
Dictyopteris polypodioides
HCl 0.1M hot
HC+AEC
Dp-F2
HPLC
48
19
5
14
5
9
DP
13
ND
Sokolova et al. (2011)
HCl 0.1M hot
HC+AEC
Dp-F4
HPLC
38
8
31
4
8
12
DP
13
ND
“
Dictyota dichotoma
HCl pH 1 hot
Ethanol ppt
R
PC
25
16
25
10
24
BC
16
d
Abdel-Fattah et al. (1978)
HCl pH 2 r.t.
PQA
EAR-0.5
GC
40
30
6
16
4
DP
13
40
Rabanal et al. (2014)
HCl pH 2 r.t.
PQA
EAR-2
GC
43
16
28
10
2
DP
33
14
“
HCl pH 2 hot
PQA
EAH1-1.5
GC
41
26
5
25
1
2
DP
19
30
“
HCl pH 2 hot
PQA
EAH2-0.5
GC
26
36
4
33
1
DP
10
42
“
HCl pH 2 hot
PQA
EAH4-0.5
GC
10
30
5
51
3
DP
5
48
“
HCl 0.1M hot
AEC+HC
DdF
GC
52
12
10
9
17
Ac
DP
2
ND
Shevchenko et al. (2017)
HCl 0.1M hot
AEC (x 2)
DdF
HPLC
58
20
12
9
Ac
DP
29
ND
Usoltseva et al. (2018b)
Dictyota divaricata
HCl 0.1M hot
AEC+HC
DdiF1
GC
61
31
4
4
Ac
DP
11
ND
Shevchenko et al. (2017)
HCl 0.1M hot
AEC+HC
DdiF2
GC
43
5
44
4
4
DP
18
ND
“
Dictyota menstrualis
Enz. pH 8
Acetone ppt
F1.0v
PC+GC
30
24
24
HexA 21
∼ 5
d
Albuquerque et al. (2004)
Enz. pH 8
Acetone ppt
F1.5v
PC+GC
31
9
47
HexA 13
∼ 16
d
“
Dictyota mertensii
Enz. pH 8
AEC
1M
Col.
26e
32
HexA 42
TB
∼ 20
d
Dietrich et al. (1995)
Enz. pH 8
AEC
2.5+3M
Col.
56e
11
HexA 33
TB
∼ 37
d
“
Enz. pH 8
Acetone ppt
ADm
GC
33
20
47
DP
∼ 22
d
Queiroz et al. (2008)
Lobophora variegata
Enz. pH 8
Acet + SEC
Lv
GC
25
75
Ac
DP
∼ 3
–
Medeiros et al. (2008)
Padina australis
CaCl2 2% hot
PQA
Fpa
GC
60
8
29
3
DP
22
21
Yuguchi et al. (2016)
Padina boryana
HCl 0.1M hot
AEC+HC
PbF
GC
61
31
4
3
Ac
DP
18
ND
Shevchenko et al. (2017)
HCl 0.1M hot
AEC (x 2)
PbF
GC
40
37
17
6
Ac
DP
19
ND
Usoltseva et al. (2018a)
Padina gymnospora
Enz. pH 8
Acet + SEC
PF1
PC+GC
36
11
7
46
DP
6
d
Silva et al. (2005)
Enz. pH 8
Acet + SEC
PF2
PC+GC
39
8
6
47
DP
3
d
“
Padina pavonica
CaCl2 2% +HCl pH2
AEC
0.3M
PC+GC
XX
X
tr.f
JL
3
20
Mian and Percival (1973)
CaCl2 2% +HCl pH2
AEC
1M
PC+GC
XX
tr.
Xf
JL
17
5
“
HCl pH 2.5 hot
AEC
Purified
PC
16
16
11
13
13
30
BC
19
d
Hussein et al. (1980)
HCl 0.1M hot
AEC
4PpF1
HPLC
43
13
9
17
17
DP
4
ND
Men’shova et al. (2012)
HCl 0.1M hot
AEC
4PpF2
HPLC
53
16
16
10
5
DP
14
ND
“
HCl 0.1M hot
AEC
4PpF3
HPLC
59
6
18
18
DP
18
ND
“
Padina tetrastomatica
H2O
CaCl2 2% ppt
PtWE1
GC
59
23
10
3
5
ND
9
Karmakar et al. (2009)
H2O
AEC+SEC
F3
GC
72
25
3
DP/IR
∼ 8
4
“
HCl 0.1M r.t.
Ext. A
GC
68
16
9
5
2
DP/IR
∼ 3
5
Karmakar et al. (2010)
HCl 0.1M +K2CO3 2%
CaCl2 2% ppt
Ext. C
GC
73
16
11
DP/IR
∼ 6
5
“
Spatoglossum asperum
H2O+CaCl2 1%
AP/R
HPLC
61
6
25
4
3
DP
21
ND
Palanisamy et al. (2017)
Spatoglossum schroederi
Enz. pH 8
Acetone ppt
Fuc. A
GC
53
18
29
DP
∼ 28
d
Queiroz et al. (2008)
Enz. pH 8
Acetone ppt
Fuc. B
GC
27
14
55
4
DP
∼ 37
d
“
Enz. pH 8
Acet.+AEC
Fuc. B
GC
28
14
56
2
TB
19
d
Menezes et al. (2018)
Stoechospermum marginatum
H2O
AEC (x 2)
F3
GC
96
2
2
DP/IR
13
–
Adhikari et al. (2006)
aKey: AEC, anion exchange chromatography; SEC, size-exclusion chromatography; HC, hydrophobic chromatography; PQA, precipitation with quaternary ammonium salts; Acet, fractional precipitation with acetone; AP/R alcohol precipitation and redissolution.bKey for the less common abbreviations: PC, paper chromatography; GC, gas chromatography; Col., colorimetric methods.cKey DP, method of Dodgson and Price (1962) or equivalent; JL, method of Jones and Letham (1954); BC, method of barium chloranilate (Lloyd, 1959); TB, method of toluidine blue; IR, estimation by area of IR bands.dThe information for the uronic acid is included in the molar ratio of monosaccharides.eFuc, Xyl and uronic acid were the only monosaccharides which could be determined.fAs galactose could not be quantified, the data is semiquantitative.
Padina pavonica was studied by Mian and Percival (1973), named then as P. pavonia. As occurred with the other seaweeds studied in that paper, the data on the table are incomplete, as Gal could not be quantified. Fraction 0.3M was rich in Fuc and Xyl, whereas fraction 1M was richer in Fuc, together with Gal. For this seaweed, Men’shova et al. (2012) carried out a seasonal study which showed that the proportion of Gal of the fucoidans increased markedly in all fractions when stepping down from spring to summer.
The fucoidans from the Dictyotales appear to be more heterogeneous than most of those of the Fucales. High proportions of Man and Rha appeared often (Table 4). However, an almost pure fucan sulfate was reported to be present in Stoechospermum marginatum (Adhikari et al., 2006) after careful purification.
Laminariales
Two species of Laminariales have been included in the early studies of Kylin (1913). They are Laminaria digitata and Saccharina lattisima (as Laminaria saccharina).
Many different species from the Laminariales have been studied thereafter, including species from four families (Agaraceae, Alariaceae, Laminariaceae, and Lessoniaceae). In order to keep up with the Silberfeld et al. (2014) taxonomy, we have included also a species from the Chorda genus (family Chordaceae) which has been recently proposed to be included in a new order, the Chordales (Starko et al., 2019). The data for the family Laminariaceae are shown in Table 5, whereas those of the remaining families appear in Table 6. It is worth noting that the species studied as Laminaria cichorioides and L. japonica are included in Table 5 as Saccharina cichorioides and S. japonica, respectively, in order to keep up with the newer taxonomy (Guiry and Guiry, 2020).
Reported compositions of the fucoidans from the family Laminariaceae (order Laminariales).
Species
Extraction
Purification/
Acronym
Monosaccharide composition (moles %)
Sulfate
UA (%)
References
Fractionationa
Methodb
Fuc
Xyl
Gal
Man
Glc
Rha
GlcA
Others
Meth.c
%
Kjelmaniella crassifolia
pH 6.5 hot
HCl pH 2 ppt
HPLC
84
5
10
ND
7
Sakai et al. (2002)
Enz. pH 4.5
AEC
F1
HPLC
30
3
49
6
4
9
Ac
DP
23
d
Song et al. (2018)
Enz. pH 4.5
AEC
F2
HPLC
47
8
15
12
1
16
Ac
DP
16
d
“
Enz. pH 4.5
AEC
F3
HPLC
67
2
23
3
1
4
DP
32
d
“
Laminaria angustata
H2O
PQA+AEC
F4
GC
90
10
EA
∼22
1
Kitamura et al. (1991)
HCl pH 2 +PQA
AEC+SEC
LA-5
GC
2
98
DP
38
3
Nishino et al. (1994b)
HCl 0.1M
PQA+AEC
LA-2
PAD
95
5
DP
56
2
Tako et al. (2010)
Laminaria bongardiana
CaCl2 2% hot
PQA+AEC
F-2
GC
53
8
20
15
3
Ac
DP
20
12
Bilan et al. (2016)
CaCl2 2% hot
PQA+AEC
F-3
GC
39
4
54
2
1
Ac
DP
26
3
“
Laminaria cichorioides
See Saccharina cichorioides
Laminaria digitata
HCl 0.01M+CaCl2 1%
GC
62
21
9
4
4
Tit
9
15
Mabeau and Kloareg (1987)
pH 7.5+CaCl2 1%
EtOH+TCA 10%
FF
GC
65
4
24
3
4
Tit
18
7
Mabeau et al. (1990)
Triton 0.5%, pH 7.5+CaCl2 1%
EtOH+TCA 10%
TF
GC
47
15
20
11
7
Tit
11
12
“
CaCl2 2% hot
PQA
GC
73
5
15
4
3
DP
27
7
Cumashi et al. (2007)
CaCl2 2% hot
GC
67
14
14
5
EA
20
10
Bittkau et al. (2020)
Laminaria hyperborea
Exudation
UF
pFuc
GC
98
2
tr.
EA
54
–
Kopplin et al. (2018)
Laminaria japonica
See Saccharina japonica
Laminaria longipes
HCl 0.1M r.t.
AEC
LlF
GC
100
DP
32
ND
Usoltseva et al. (2019)
Laminaria religiosa
HCl pH 2 hot
PQA
Fr 0.5
GC
34
12
14
21
19
DP
9
35
Koo et al. (2001)
HCl pH 2 hot
PQA
Fr. 3
GC
61
1
28
7
3
DP
39
18
“
Macrocystis pyrifera
Exudation
AP/R
PC+CC
92
2
6
tr.
19
–
Schweiger (1962)
SigmaTM
HPLC
79
3
12
3
3
DP
27
5
Zhang et al. (2015)
HCl pH 1 hot
Acetone ppt
AFS
HPLC
80
17
3
DP
24
–
Lorbeer et al. (2017)
Saccharina cichorioides
HCl 0.4%+H2O
HC
L.c.F-2
HPLC
81
2
4
2
3
8
DP
∼35
ND
Zvyagintseva et al. (1999)
HCl 0.4% r.t.
HC
Lc2-F1
HPLC
72
7
8
8
5
DP
∼30
ND
Zvyagintseva et al. (2003)
HCl 0.4% +H2O
HC
Lc2-F2
HPLC
100
DP
∼36
ND
“
HCl pH 2-2.3 hot
AEC
Lc-F2
HPLC
98
2
DP
30
ND
Anastyuk et al. (2010)
HCl 0.1M r.t.
AEC
Sc-F1
HPLC
95
5
DP
21
ND
Vishchuk et al. (2013)
HCl 0.1M r.t.
AEC
Sc-F2
HPLC
100
DP
39
ND
“
HCl pH 2-2.3
AEC
ScF
HPLC
89
2
6
3
DP
26
ND
Prokofjeva et al. (2013)
HCl 0.1M r.t.
AEC
ScF
GC
98
2
DP
36
ND
Usoltseva et al. (2019)
Saccharina gurjanovae
HCl pH 2-2.3
AEC
SgGF
HPLC
64
21
15
Ac
DP
28
ND
Prokofjeva et al. (2013)
CaCl2 2% hot
AEC (x 2)
SgF
GC
76
24
Ac
DP
25
ND
Shevchenko et al. (2015)
Saccharina japonica
HCl 0.4% +H2O
HC
L.j.-F-2
HPLC
94
2
3
1
ND
ND
Zvyagintseva et al. (1999)
HCl 0.4% r.t.
HC
Lj1-F1
HPLC
55
7
26
6
3
3
ND
ND
Zvyagintseva et al. (2003)
HCl 0.4% +H2O
HC
Lj1-F2
HPLC
84
1
12
1
2
DP
∼25
ND
“
HCl pH 3 r.t.
AEC
L
HPLC
61
5
14
16
4
DP
21
18
Ozawa et al. (2006)
HCl pH 3 r.t.
AEC
GA
HPLC
90
10
DP
38
1
“
HCl 0.1M hot
AEC
Sj-F1
HPLC
53
1
29
15
2
DP
10
ND
Vishchuk et al. (2011)
HCl 0.1M hot
AEC
Sj-F2
HPLC
61
2
33
1
3
Ac
DP
23
ND
“
HCl 0.2M hot
Sterile
HPLC
41
8
14
12
14
11
ND
ND
Skriptsova et al. (2012)
HCl 0.2M hot
Reprod.
HPLC
25
3
13
4
48
7
ND
ND
“
HCl 0.1M hot
AEC
Sj-sF2
HPLC
62
6
21
9
2
DP
21
ND
Vishchuk et al. (2012)
HCl 0.1M hot
AEC
Sj-fF2
HPLC
58
37
5
DP
23
ND
“
HCl pH 2-2.3
AEC
SjGF
HPLC
50
1
44
5
Ac
DP
23
ND
Prokofjeva et al. (2013)
HCl pH 2.5 hot
B
CZE
54
3
29
3
1
10
ND
d
Guo et al. (2013)
H2O hot
CaCl2 1%+AP/R
LJF
HPLC
34
2
37
23
1
3
DP
14
3
Qu et al. (2014)
HCO2H 0.1%, PTe
CaCl2 1%
HPLC
57
17
21
5
DP
24
10
Saravana et al. (2016)
Saccharina latissima
CaCl2 2% hot
PQA
GC
80
3
10
2
5
DP
30
5
Cumashi et al. (2007)
CaCl2 2% hot
PQA+AEC
F-1.0
GC
46
5
32
14
3
DP
16
23
Bilan et al. (2010)
CaCl2 2% hot
PQA+AEC
F-1.25
GC
78
2
18
2
DP
37
2
“
CaCl2 2% hot
AEC
B06-F2
GC
56
14
14
13
3
EA
6
–
Ehrig and Alban (2015)
CaCl2 2% hot
AEC
B06-F3
GC
76
3
20
1
EA
16
–
“
CaCl2 2% hot
GC
84
7
7
2
EA
29
6
Bittkau et al. (2020)
Enz.pH6 + CaCl2 2%
AEC
SlF3
PAD
63
3
27
2
HexA 4
DP
46
d
Nguyen et al. (2020)
Saccharina longicruris
CaCl2 2% +HCl 0.01M
B
ND
EA
14
8
Rioux et al. (2007)
aKey: AEC, anion exchange chromatography; SEC, size-exclusion chromatography; HC, hydrophobic chromatography; PQA, precipitation with quaternary ammonium salts; AP/R alcohol precipitation and redissolution; UF, ultrafiltration.bKey for the less common abbreviations: PAD, HPAEC with pulse amperometric detector; PC, paper chromatography; GC, gas chromatography; CC, column chromatography on cellulose; CZE, capillary zone electrophoresis.cKey DP, method of Dodgson and Price (1962) or equivalent; EA, elemental analysis by different methods; Tit, titration with cetylpyridinium chloride, pH 1.5 (Scott, 1960).dThe information for the uronic acid is included in the molar ratio of monosaccharides.eHigh pressure and temperature have been applied.
Reported compositions of the fucoidans from the order Laminariales (families other than the Laminariaceae).
Species
Extraction
Purification/
Acronym
Monosaccharide composition (moles %)
Sulfate
UA (%)
References
Fractionationa
Methodb
Fuc
Xyl
Gal
Man
Glc
Rha
GlcA
Others
Methodc
%
Family Agaraceae
Costaria costata
HCl pH 2-2.3 hot
FLM7
HPLC
62
4
18
5
7
4
DP
12
ND
Imbs et al. (2009)
HCl 0.1M hot
AEC
CcF
HPLC
51
3
43
tr.
3
Ac
DP
19
ND
Ermakova et al. (2011)
HCl pH 2-2.3 r.t.
HC
F1.5
HPLC
70
20
7
3
DP
24
d
Imbs et al. (2011)
HCl pH 2-2.3 hot
AEC
5F2
GC
30
16
8
15
15
DP
15
d
Anastyuk et al. (2012a)
HCl pH 2-2.3 hot
AEC
5F3
GC
40
12
21
12
6
7
DP
15
d
“
HCl pH 2-2.3
CcGF
HPLC
63
30
3
2
Ac
DP
23
ND
Prokofjeva et al. (2013)
Enz. pH 4.5
AP/R+AEC
F2
GC
17
7
8
61
8
Grav
1
ND
Wang et al. (2014)
Enz. pH 4.5
AP/R+AEC
F4
GC
47
17
17
12
8
Grav
23
ND
“
Enz. pH 4.5
AEC
6F1
GC
21
11
20
30
7
10
DP
9
4
Liu et al. (2018)
Enz. pH 4.5
AEC
6F2
GC
31
15
9
26
11
8
DP
10
6
“
Family Alariaceae
Alaria angusta
HCl 0.1M hot
HC+AEC
AaF2
HPLC
75
7
18
DP
14
ND
Menshova et al. (2015)
HCl 0.1M hot
HC+AEC
AaF3
HPLC
53
47
Ac
DP
24
ND
“
Alaria marginata
HCl 0.1M hot
HC+AEC
AmF2
HPLC
81
9
11
DP
21
ND
Usoltseva et al. (2016)
HCl 0.1M hot
HC+AEC
AmF3
HPLC
48
5
47
Ac
DP
28
ND
“
Alaria ochotensis
HCl 0.2M hot
Sterile
HPLC
18
4
10
4
59
6
ND
ND
Skriptsova et al. (2012)
HCl 0.2M hot
Reprod.
HPLC
25
3
23
5
40
4
ND
ND
“
HCl pH 2-2.3
AEC
AoGF
HPLC
54
38
8
DP
24
ND
Prokofjeva et al. (2013)
Undaria pinnatifida
HCl 0.15M
AEC+SEC
CF-4B
GC
48
52
EA
32
2
Lee et al. (2004)
H2SO4 1% r.t.
AEC
F2M
GC
54
45
1
EA
∼ 28
1
Hemmingson et al. (2006)
HCl 0.2M hot
UF
F > 30K
HPLC
64
32
4
DP
32
ND
You et al. (2010)
HCl 0.1M r.t.
AP/R+AEC
GC
51
4
45
Ac
EA
30
ND
Synytsya et al. (2010)
HCl 0.1M hot
AEC
Up-F1
HPLC
59
2
30
8
1
DP
14
ND
Vishchuk et al. (2011)
HCl 0.1M hot
AEC
Up-F2
HPLC
51
48
1
Ac
DP
29
ND
“
CaCl2 2% hot
PQA+AEC
F1
GC
49
4
38
7
3
DP
7
4
Mak et al. (2013)
CaCl2 2% hot
PQA+AEC
F3
GC
60
2
29
7
3
DP
25
1
“
HCl 0.2M r.t.
GC
53
42
2
3
ND
2
Wozniak et al. (2015)
SigmaTM
PAD
55
45
DP
26
2
Lu et al. (2018)
H2O+CaCl2 2%
SEC
F300
HPLC
56
7
35
2
DP
20
5
Koh et al. (2019)
Family Chordaceaee
Chorda filum
CaCl2 2% hot
AEC
A-2
GC
95
1
1
1
2
Ac
DP
26
–
Chizhov et al. (1999)
Na2CO3 3%
AEC
C-1
GC
83
3
1
8
4
DP
13
5
“
Na2CO3 3%
AEC
C-2
GC
72
11
5
7
4
DP
13
3
“
Family Lessoniaceae
Ecklonia cava
HCl 0.1M hot
AEC
Ec-F1
HPLC
70
15
4
11
DP
19
ND
Ermakova et al. (2011)
HCl 0.1M hot
AEC
Ec-F2
HPLC
57
16
23
4
DP
22
ND
“
Enz.+CaCl2 4M
PQA+AEC
F1
PAD
53
8
33
2
4
DP
20
16
Lee et al. (2012)
Enz.+CaCl2 4M
PQA+AEC
F2
PAD
60
4
31
1
4
DP
16
14
“
Enz.+CaCl2 4M
PQA+AEC
F3
PAD
78
8
10
2
2
DP
39
9
“
Ecklonia kurome
H2O+PQA
AEC+SEC
B-I
GC
34
34
13
18
DP
19
30
Nishino et al. (1989)
H2O+PQA
AEC+SEC
C-I
GC
97
3
DP
47
2
“
H2O+PQA
AEC+SEC
C-II
GC
83
17
DP
43
4
“
Ecklonia maxima
H2O hot
CaCl2 1% +AP/R
EMF
HPLC
63
2
12
17
3
3
DP
21
tr.
Qu et al. (2014)
Ecklonia radiata
HCl pH 2 hot
CaCl2 0.5%
6 min
HPLC
57
6
37
DP
22
2
Lorbeer et al. (2015)
HCl pH 1 hot
Acetone ppt
AFS
HPLC
84
3
8
3
3
DP
28
1
Lorbeer et al. (2017)
Eisenia bicyclis
HCl 0.1M hot
AEC
EbF
HPLC
67
7
20
7
DP
14
ND
Ermakova et al. (2013)
Lessonia nigrescens
HCl pH 2 hot
B-Stipes
PC+GC
63
14
13
10
JL
6
29
Percival et al. (1983)
HCl pH 2 hot
B-Frond
PC+GC
82
12
6
JL
7
17
“
HCl pH 2+ Na2CO3 3%
AEC
DF
PC+GC
57
13
21
9
JL
ND
ND
“
H2O hot
CaCl2 1% +AP/R
LNF
HPLC
65
11
14
4
6
DP
17
–
Qu et al. (2014)
Lessonia trabeculata
H2O hot
CaCl2 1% +AP/R
LTF
HPLC
53
3
25
11
4
4
DP
16
tr.
Qu et al. (2014)
Lessonia vadosa
CaCl2 2%+HCl 0.25M
GC
∼100
tr.
tr.
DP
38
–
Chandía and Matsuhiro (2008)
Lessonia sp.
CaCl2 2% hot
AEC
B’-F1
GC
(~100
tr.
tr.
DP
37
4
Leal et al. (2018)
aKey: AEC, anion exchange chromatography; SEC, size-exclusion chromatography; HC, hydrophobic chromatography; PQA, precipitation with quaternary ammonium salts; AP/R alcohol precipitation and redissolution; UF, ultrafiltration.bKey for the less common abbreviations: PAD, HPAEC with pulse amperometric detector; GC, gas chromatography.cKey: JL, method of Jones and Letham (1954); DP, method of Dodgson and Price (1962) or equivalent; IC, ion chromatography; EA, elemental analysis; Grav, gravimetric method.dThe information for the uronic acid is included in the molar ratio of monosaccharides.eThis family has been included recently in a separate order, the Chordales (Starko et al., 2019).
Many galactofucans have been found within the Laminariaceae family, usually with low proportions of Xyl or Man. However, several fractions containing almost pure fucans have been found in Laminaria angustata, L. hyperborea, Macrocystis pyrifera, Saccharina cichorioides, and S. japonica (Table 5). For L. angustata, Nishino et al. (1994b) have isolated a homogalactan sulfate, probably in the only case that an almost fucose-free product is found within the “fucoidan” fractions of brown seaweeds. The trend showing mixtures of polysaccharides separable by charge also occurs for the products from the Laminariales: usually heterogeneous polymers, containing high proportions of uronic acids, and low sulfation appear in the early-eluting fractions of anion exchange chromatography, whereas highly sulfated fucans or galactofucans appear in the late-eluting fractions.
Seasonal differences were also observed: for Costaria costata, Imbs et al. (2009) determined that the proportion of Fuc, Gal, Glc, and sulfate increased from spring to summer, whereas those of Man, Rha, and Xyl decreased. This trend is similar to that observed by Men’shova et al. (2012) for Padina pavonica (see above). In another study, carried out for Saccharina cichorioides (as Laminaria cichorioides), it has been shown that after the summer, and through fall, the proportion of Fuc decreases again, whereas that of Man increases clearly (Anastyuk et al., 2010).
On the basis of chemical degradations and NMR spectroscopy, Bilan et al. (2010) arrived to many structural features of the fucoidans from Saccharina lattisima. Ehrig and Alban (2015) have shown the large effect of the marine habitat and season on the characteristics of the isolated fucoidans of this seaweed. Samples picked up in the Baltic Sea showed more laminaran contamination and lower fucoidan yields, fucose, and sulfate content than those collected around the Faroe Islands (regardless of the season), although the uronic acid content was similar. Regarding the season effects, the proportion of sulfate was higher in fucoidans from seaweeds collected in September than in May. Anion-exchange chromatography separation showed that only from the September-collected seaweed it was possible to obtain high yields of a high-fucose fraction with the highest biological activity. However, in a further work from the same group (Bittkau et al., 2020), the authors have isolated such a fraction with high fucose and sulfate content from the same North Atlantic location, in July without the need of any purification, suggesting that the year of collection has a major effect on the composition of the isolated fucoidans.
A study carried out with an unidentified species of Alaria (Alaria sp., Vishchuk et al., 2012) was later ascertained as being A. ochotensis (Prokofjeva et al., 2013). In the Alaria species studied so far, it is noteworthy to mention the presence of fucogalactans with approximately equal proportions of Fuc and Gal (Table 6).
For Costaria costata, high proportions of Man have been encountered in the polymers, especially in the less charged fractions isolated in some studies (Wang et al., 2014). In any case, Man appears conspicuously in most of the studies carried out on fucoidans of any origin.
The polysaccharides from Undaria pinnatifida were studied by many research groups, probably due to the fact that this seaweed, native from northeastern Asia, is very invasive and now is widespread all around the world (Casas et al., 2004; Thornber et al., 2004). It is worth noting that most of the studies have shown the presence of a galactofucan with high proportions of Gal, sometimes leveling out with Fuc. The proportion of other sugars (Man, Xyl and uronic acids) is usually low, whereas the proportion of sulfate is considerable, but lower than those of other species (Table 6).
Other Orders
The analysis of the fucoidans of different species of the order Ectocarpales appears in Table 7. In this survey, only reports for ten different species (belonging to three families) of the order have been found. Highly sulfated galactofucans or homofucans coexist with polysaccharides containing significant proportions of Man, GlcA and/or Xyl.
Reported compositions of the fucoidans from the orders Ascoseirales, Desmarestiales, Ectocarpales, Ralfsiales, and Scytothamnales.
Species
Extraction
Purification/
Acronym
Monosaccharide composition (moles %)
Sulfate
UA (%)
References
Fractionationa
Methodb
Fuc
Xyl
Gal
Man
Glc
Rha
GlcA
Others
Methodc
%
Ascoseirales
Ascoseira mirabilis
CaCl2 2% hot
AEC+SEC
1AF
PC+GC
29
9
19
9
10
25
JL
12
d,e
Finch et al. (1986)
Na2CO3 3% hot
AEC+SEC
3AF
PC+GC
17
9
31
14
9
17
JL
8
d,e
“
Desmarestiales
Desmarestia aculeata
Na2CO3 3% hot
GC+PC
21
3
41
35
JL
Low
d
Percival and Young (1974)
Desmarestia firma
H2O
AEC
F0.3M
GC+PC
X
X
X
∼50f
X
ManA X
JL
1
17
Carlberg et al. (1978)
Desmarestia ligulata
H2O
AEC
F0.2M
GC
52
3
5
1
38
JL
3
d
“
H2O
AEC
F0.5M
GC
66
7
18
9
JL
20
4
“
Desmarestia viridis
HCl 0.1M hot
AEC+HC
DvF
GC
63
13
17
7
Ac
DP
12
ND
Shevchenko et al. (2017)
Ectocarpales
Family Adenocystaceae
Adenocystis utricularis
HCl pH 2 r.t.
PQA
EA1-5
GC
47
4
9
26
6
8
DP
5
42
Ponce et al. (2003)
HCl pH 2 r.t.
PQA
EA1-20
GC
83
15
1
DP
23
4
“
HCl pH 2 hot
PQA
EA2-5
GC
58
3
6
29
1
3
DP
6
31
“
HCl pH 2 hot
PQA
EA2-20
GC
75
1
21
1
1
1
DP
21
6
“
Family Chordariaceae
Cladosiphon okamuranus
HCl pH3
CaCl2 3.5%+AEC
GC
86
14
Ac
DP
∼ 12
d
Nagaoka et al. (1999)
ND
GC
91
2
7
DP
15
23
Cumashi et al. (2007)
HCl 0.05M r.t.
CaCl2 0.1M
CAF
PAD
99
1
Ac
DP
∼ 16
12
Teruya et al. (2009)
ND
CE
GC
95
3
1
DP
15
9
Lim et al. (2019)
Chordaria flagelliformis
CaCl2 2% hot
AEC
F2
GC
80
5
12
2
Ac
DP
18
16
Bilan et al. (2008)
CaCl2 2% hot
AEC
F3
GC
96
4
Ac
DP
27
13
“
CaCl2 2% hot
AEC
F4
GC
100
Ac
DP
27
10
“
Dictyosiphon foeniculaceus
CaCl2 2% hot
GC
39
32
16
6
5
EA
9
10
Bittkau et al. (2020)
Leathesia difformis
HCl pH 2 r.t.
Ea
GC
90
6
4
DP
6
3
Feldman et al. (1999)
Nemacystus decipiens
H2O, Pressure
HN0
PAD
66
10
3
3
9
Fru 9,GalN 2
IC
20
36
Li et al. (2017)
H2O
CaCl2 3M+AEC
NP1
HPLC
74
3
5
2
15
DP
4
d
Cui et al. (2018)
H2O+CaCl2
AEC+SEC
NP2
HPLC
76
2
2
20
Ac
DP
19
d
“
Papenfussiella lutea
H2SO4 1% r.t.
GC
55
4
9
1
31
ND
5
Wozniak et al. (2015)
Punctaria plantaginea
CaCl2 2% hot
PQA
GC
69
27
4
DP
19
2
Bilan et al. (2014)
Family Scytosiphonaceae
Chnoospora minima
Enzymes pH 4.5 and 8
CaCl2+AEC
F2,1
PAD
19
38
7
NIg 31, Ara 3
DP
5
ND
Fernando et al. (2017)
Enzymes pH 4.5 and 8
CaCl2+AEC
F2,4
PAD
79
3
NI 18
DP
34
ND
“
Enzymes pH 4.5
CaCl2 5M
CMF
PAD
65
6
9
1
NI 19
DP
24
ND
Fernando et al. (2018)
Scytosiphon lomentaria
HCl pH 2 r.t.
PQA
A5
GC
38
15
15
24
3
5
DP
6
20
Ponce et al. (2019)
HCl pH 2 r.t.
PQA
A30
GC
88
12
DP
29
2
“
Ralfsiales
Analipus japonicus
CaCl2 2% hot
PQA+AEC
F1
GC
74
12
12
2
Ac
DP
13
12
Bilan et al. (2007)
CaCl2 2% hot
PQA+AEC
F2
GC
84
4
11
Ac
DP
23
6
“
Scytothamnales
Scytothamnus australis
H2SO4 1% r.t.
GC
92
3
2
1
2
ND
2
Wozniak et al. (2015)
Splachnidium rugosum
CaCl2 2% hot
GC
86
7
3
2
2
ND
2
“
aKey: AEC, anion exchange chromatography; SEC, size-exclusion chromatography; HC, hydrophobic chromatography; PQA, precipitation with quaternary ammonium salts; CE cation exchange.bKey for the less common abbreviations: PAD, HPAEC with pulse amperometric detector; GC, gas chromatography.cKey: JL, method of Jones and Letham (1954); DP, method of Dodgson and Price (1962) or equivalent; IC, ion chromatography; EA, elemental analysis.dThe information for the uronic acid is included in the molar ratio of monosaccharides.eEven after purification, these samples contain 10–12% of alginic acid.fOnly the proportion of Glc is indicated. The remaining monosaccharides were not quantified.gNI = sugar not identified.
The analysis of the fucoidans from four species from the Desmarestiales is also shown in Table 7. It should be taken into account that these seaweeds contain free sulfuric acid in their vacuoles (Carlberg et al., 1978), making them very labile when taken out from the marine environment. This requires special techniques in order to obtain neutral extracts unaffected by the strong acid.
To the best of our knowledge, the fucoidans from only one species from the Ascoseirales and Ralfsiales, and two of the Scytothamnales have been studied (Table 7). The fucoidans from the three samples from the Ralfsiales and Scytothamnales appear to be particularly rich in Fuc and poor in uronic acids, whereas the Ascoseira sample was quite heterogeneous (Finch et al., 1986, Table 7).
Concluding Remarks
The current review has surveyed most of the compositional data on fucoidans extracted from different species, in many cases after purification; more than 100 species were screened through the literature. Besides the obvious purpose of providing a reliable source of compositional data gathered in a set of tables, this review attempted to foresee if there is any correlation of these compositional data with their taxonomy, or if other factors are more important than the taxonomic origin.
These general considerations can be deduced from the analysis of the compositional data:
Separation by charge is the most efficient method to obtain “pure” fucoidan fractions. Either using anion-exchange chromatography with increasing concentrations of salt as eluant, or by precipitating with cationic detergents and redissolving at increasing ionic strengths, two main type of polymers can be separated: (a) those appearing at low ionic strengths, usually highly heterogeneous in their monosaccharidic composition (containing Fuc, Xyl, Gal, Man, Rha, GlcA), with low-sulfate content, and high uronic acid content, and b) those appearing at high ionic strengths, containing mainly Fuc, accompanied with variable proportions of Gal, highly sulfated and containing little (or none) uronic acids. Fractions containing intermediate proportions of both polysaccharides appear at medium ionic strengths. Figure 3 depicts the composition of fractions belonging to each of the first groups from selected seaweeds, showing clearly the marked differences between both groups. This behavior is observed for samples from the orders Fucales, Laminariales, Ascoseirales, Desmarestiales, Ectocarpales, and Ralfsiales (Mian and Percival, 1973; Carlberg et al., 1978; Bilan et al., 2002, 2013, 2016, 2018; Ponce et al., 2003, 2019; Ozawa et al., 2006; Mak et al., 2013); however, for the Dictyotales, the trend is obscured due to the abundance of Man and/or uronic acids in the products separated at each ionic strength (Table 4). It has been postulated that the biological activity is concentrated on the galactofucan components (Ponce et al., 2003, 2019; Croci et al., 2011).
Difference in selected reported compositions of fucoidans submitted to charge-based separation methods. Fractions on the left side were eluted or redissolved at low ionic strengths, whereas those on the right side were eluted or redissolved at higher ionic strengths. Upper panel, neutral monosaccharide composition (mol/100 mols); lower panel, sulfate and uronic acid content. The data were reported by Koo et al. (2001), Bilan et al. (2002, 2008, 2010, 2013, 2018), and Ponce et al. (2003, 2019).
Acetate esters of the fucoidans are very common. As a matter of fact, this constituent has been found in almost every sample where it was searched. Determinations of acetyl groups are not very common, as they are only encountered through NMR spectra or specific colorimetric techniques. They are labile enough in mild alkaline or acid media as to get undetected when using some extraction procedures (Bernhard and Hammett, 1953; Wuts and Greene, 2006). Anyway, almost all of the seven tables report acetyl groups on some species. It is highly probable that searching in other species would have resulted in many more positive results.
In some cases, Man and Rha appear together, usually in fractions with lower sulfate contents. For Man, structural explanations have already been reported in terms of fucomannoglucuronans (Bilan et al., 2010), but for Rha no structural function has been found so far. Rha seems to appear in higher proportions within the order Dictyotales and the family Sargassaceae (Fucales).
The Dictyotales appear to be the most “atypical” order, as usually large proportions of Man and uronic acids appear. In one species which was highly fractionated, Man becomes the most important monosaccharide in the low-charged fractions, and it is still important in the fractions with more sulfate groups (Table 4; Rabanal et al., 2014). However, fractions with high proportions of monosaccharides different than Fuc were found in most of the taxa studied so far (see Tables).
The uronic acid content should be considered with due care. Sometimes it corresponds to GlcA actually comprising the fucoidan structure, but sometimes it corresponds to contamination with alginic acid (e.g., Finch et al., 1986; Lorbeer et al., 2017), a polysaccharide present in all of the brown seaweeds studied so far. By the same token, the Glc present in the samples should almost certainly correspond to contaminating laminarans (Lorbeer et al., 2017; Mateos-Aparicio et al., 2018). Only in a few cases, Glc has been shown to be part of the fucoidan structure (e.g., Duarte et al., 2001).
There are several factors to consider when comparing the compositional data of fucoidans from different seaweeds and research groups. The taxon is just one of them. Others like geographical location, year and season of harvest of the seaweed, extraction and purification methods, analytical methods, different parts or reproductive stages of the seaweeds are also of paramount importance in defining the final characteristics.
The geographic site of harvesting appears to be very important: Zvyagintseva et al. (2003) found marked differences between the fucoidans of Fucus evanescens collected in different spots of the southern Okhotsk Sea. Ehrig and Alban (2015) also found a significant difference between the composition and yields of fucoidans of Saccharina lattisima samples collected in the North Atlantic and in the Baltic Sea. This factor, together with the year of collection might explain the large differences in composition found for species studied by different groups (or at different times) even with similar extraction and purification procedures.
The season of harvesting has also influence over the composition of the fucoidans: a trend with increasing yields, and proportions of sulfate, Fuc, Gal and Glc (together with a decrease in the Man and Rha content) is observed as the collection month progressed from March to October, in the Northern Hemisphere (Imbs et al., 2009; Anastyuk et al., 2010; Men’shova et al., 2012; Ehrig and Alban, 2015).
The effect of the extraction conditions is more controversial: Ponce et al. (2003) and Wozniak et al. (2015) found very little differences when switching the extraction solvent from water to CaCl2 to diluted HCl. Alboofetileh et al. (2019b) found differences in yield and in sulfate content but a very similar monosaccharide composition using enzymes, ultrasound, or both combined. Rodríguez-Jasso et al. (2011) found a significant difference in composition and yields when changing the time and the pressure of a microwave-assisted water extraction. Nguyen et al. (2020) have shown a sharply different composition of the chemically and enzymatically-extracted crude products, being the latters richer in alginic acid and sulfate/Fuc ratios. After purification, the compositions might level off. However, the enzyme-aided extraction, also used by other groups (Dietrich et al., 1995; Albuquerque et al., 2004; Silva et al., 2005; Medeiros et al., 2008; Queiroz et al., 2008; Costa et al., 2011; Camara et al., 2011; Lee et al., 2012; Wang et al., 2014; Hu et al., 2016; Monsur et al., 2017; Fernando et al., 2017, 2018; Liu et al., 2018; Menezes et al., 2018; Song et al., 2018; Jayawardena et al., 2019; Alboofetileh et al., 2019a,b) appears to be an interesting prospect, considering cleaner chemical issues and the possibility of finding enhanced biological activities in comparison with chemically extracted products (Nguyen et al., 2020).
Some differences were found between the fucoidans isolated from reproductive and sterile tissue of five different seaweeds (Skriptsova et al., 2012, see Tables 1, 2, 5, 6). Usually the reproductive tissue is less heterogeneous, and carries more Fuc and less Glc than the sterile tissue. Regarding the extraction of fucoidans from different parts of the seaweeds, Percival et al. (1983) extracted separately the polysaccharides from fronds and stipes from Lessonia nigrescens, whereas Wozniak et al. (2015) compared the fucoidans isolated from reproductive structures and from vegetative structures in Marginariella boryana. The fucoidans from stipes and the vegetative structures, respectively, appear to be more heterogeneous (less Fuc and more uronic acids).
In order to obtain fucoidan samples devoid of contaminants, the best results were obtained by carrying out the extractions with dilute HCl or CaCl2, or using these agents after the extraction (for instance enzymatic) in order to precipitate the alginate in the first place, followed by a careful separation by charge (anion exchange chromatography eluting with increasing ionic strength, or precipitation with quaternary ammonium salts followed by redissolution with increasing ionic strengths). Further purification of each fraction by size-exclusion chromatography usually yield fucoidans devoid of alginic acid or laminaran contaminants.
The conclusion is that with so many variables determining the composition of the fucoidans, the subtle differences that might appear among the different higher taxa (order, family) surveyed in this review are overridden. Probably, comparisons carried out in the same labs with the same methods might help, or more profound structural studies might throw light on chemotaxonomical issues in the future.
Author Contributions
NP was involved in the conceptualization, formal analysis, investigation, writing, and visualization of this work. CS was involved in the conceptualization, formal analysis, writing, visualization, and funding of this work. Both authors contributed to the article and approved the submitted version.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Funding. This work was supported by grants from the University of Buenos Aires (20020170100255BA), National Research Council of Argentina-CONICET (PIP 298/14 and P-UE 22920160100068CO), and ANPCyT-Argentina (PICT 2017-1675).
We are indebted to Dr. María C. Rodríguez for her help on botanical/psychological issues, and to Dr. Marina Ciancia for her kind invitation to participate in this issue.
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