Tabanidae, normally called horse flies or deer flies, is an ubiquity family, and the most diverse family-level clade that has more than 4000 species distributed in 156 genera worldwide (Figure 1A; Pape et al., 2011). They are stout-bodied flies, with larger first flagellomere and 4-8 apical flagellomeres; legs with two apical spurs on midtibia, tarsi with pulvilliform empodium; wing venation with R4 and R5 enclose wing apex, form a large ‘Y’ across the wing tip; cell br, bm and d large, cell cup closed near wing margin; calypters almost always well developed (Colless and McAlpine, 1991; Burger, 2009). Tabanidae is type family of Tabanidae which characteristiced by the presence of a venom canal of the larval mandible (Kerr, 2010; Morita et al., 2016).

Tabanids are relatively scarce in the fossil record, but in Cenozoic, they are quite abundant as fossil recorded from Miocene of Florissant, from North American, Germany, French, and Switzerland Oligocene, from England and Baltic amber Eocene/Oligocene, Pliocene from Europe and Africa (Martins-Neto, 2003). The oldest record of a true tabanid was reported from the Lower Cretaceous Durlston Formation of England. Till now, five species of tabanids was recorded in the Early Cretaceous and one species primitively in Therevidae was moved to the tabanid genus Cratotabanus Martins-Neto and Santos (1994; Ren, 1998; Martins-Neto, 2003; Mostovski et al., 2003; Zhang, 2012). Fossils from the Late Cretaceous are quite rare, with only one species and genus from Late Cretaceous of New Jersey amber, together with two newly described species in Burmese amber (Grimaldi et al., 2011; Grimaldi, 2016). Flower-feeding tabanids (Pangoniinae) appear at least in the Early Cretaceous (Martins-Neto and Santos, 1994; Ren, 1998; Zhang, 2012). A recent molecular analysis calibrated using several key fossils support that the divergence of Tabanidae and their sister clade Athericidae, in the Early Cretaceous, approximately 135 Ma (Morita et al., 2016).

Nemestrinidae commonly called tangle-veined flies, is cosmopolitan but quite a small group of brachycerous flies, with about 300 extant species in over 20 genera (Figure 1B; Bernardi, 1973; Mostovski and Martínez-Delclòs, 2000). They are usually medium-sized flies with body stout and dense pilosity, wings are usually longer than body (Wedmann, 2007; Woodley, 2009). They can be easily recognized by a so-called diagonal vein, the compound diagonal vein obliquely aligned through the wing; they also have some characteristics including tibiae without apical spurs, empodium pulvilliform, and one segmented cercus and flagellum often formed into a slender stylus (Yeates, 1994; Wedmann, 2007). Fossil tangle-veined flies are quite abundant since Mesozoic, many nemestrinids were found in Late Jurassic and Early Cretaceous, and some Cenozoic nemestrinids were described, mainly from the Oligocene of Florissant, USA. Ansorge and Mostovski (2000) listed an updated list of all taxa of Nemestrinidae, and additional taxa have been described from the Eocene of Germany (Wedmann, 2007), mid-Cretaceous Burmese amber (Grimaldi, 2016; Zhang et al., 2017), and a doubtable genus without diagonal vein from the Late Jurassic of China (Zhang et al., 2008). The oldest fossil nemestrinids are from the Late Jurassic of Karabastau, Kazakhstan (Rohdendorf, 1968; Mostovski, 1998). Ansorge and Mostovski (2000) hypothesized that the family Nemestrinidae probably originated in the Late Triassic or Early Jurassic, as the oldest fossil Nemestrinidae appeared in Early Jurassic and fossil nemestrinids demonstrate a high taxonomic diversity since the Middle-Late Jurassic. Nemestrinidae is thought to be a sister group of Apioceridae in Nemestrinoidea supported by their parasitic larval lifestyle (Woodley, 1989; Yeates, 2002).

Bombyliidae (bee flies) is a quite diverse and widely distributed family of Asiloidea. It is a cosmopolitan group and a quite large family that comprising over 4500 described extant species around the world (Figure 1C; Evenhuis, 1994; Evenhuis and Greathead, 2003; Wedmann and Yeates, 2008). They are commonly robust flies, often with long projecting proboscis and usually densely hairs (Colless and McAlpine, 1991; Greathead et al., 2009). They feed on nectar as well as pollen, many of them using a long proboscis to probe flowers (Grimaldi, 2016). The fossil of adult bee flies can be distinguished by the following features: antenna usually with flagellomere coniform, usually with one or two flagellomeres and a terminal bristlelike stylus; wing R2+3 and R4 usually strongly curved distally, meeting costa at about a right angle; R4+5 branched, R4 and R5 usually encompass wing tip; three (rarely two) posterior cells (Greathead et al., 2009). Fossil bee flies are quite abundant in Cenozoic, especially in the Oligocene and Eocene. Till now, about 70 species in about 40 genera have been described from Florissant of USA, France, Germany, and Dominican and Baltic ambers. The fossil record of Bombyliidae has been reviewed by Hull (1973) and Evenhuis (1994), and new taxa was recently described by Nel and De Ploëg (2004), Nel (2006), and Wedmann and Yeates (2008).

Grimaldi (2016) suggested that the radiation age of Bombyliidae is the Late Cretaceous, but Lamas and Nihei (2007) suggested a Middle Jurassic age based on the molecular phylogenetic analysis. Molecular models and biogeography support a Late Mesozoic diversification of asiloids, with Bombyliidae at the base of the Asiloidea (Winterton et al., 2015; Grimaldi, 2016). Unambiguous Mesozoic bombyliids are extremely rare. Recently, some definitive new records of Bombyliidae in mid-Cretaceous Burmese amber show that bombyliids have already diversified, and these fossils provide new insights into the early evolution of Cretaceous bee flies (Shi et al., 2012; Grimaldi, 2016; Zhang et al., 2016b).

Eremochaetidae is a Mesozoic extinct family that was established by Ussatchov based on two species in two different genera (Ussatchov, 1968). Eremochaetidae is a quite rare family that was found only in Late Mesozoic, mainly in Early Cretaceous. Till now, only 15 species in nine genera have been described in China, Kazakhstan, Mongolia, Russia and Burmese amber (Ussatchov, 1968; Kovalev, 1989; Ren and Guo, 1995; Mostovski, 1996; Ren, 1998; Zhang, 2014; Zhang et al., 2014, 2016b). All eremochaetids have the characters: eyes very large, occupying the greater part of the head; thorax short and convex; Sc is stout, R1 is very long; cross-vein is absent, causing the vein R4+5 (sometimes R2+3 and R4+5) to arise from cell d; the ovipositor is needle-shaped in all female eremochaetids (Ussatchov, 1968; Zhang et al., 2014). Zhang (2014) described and illustrated the structures of the male genitalia for the first time, and reasoned that these characteristics probably represent the base type of the primitive lower Orthorrhapha of Brachycera. The latest occurrence of eremochaetids is from the mid-Cretaceous Burmese amber (Zhang et al., 2016a). The highly developed, hypodermic-like ovipositor and enlarged tridactylous characteristic in pretarsus supported their endoparasitoid life, and their primitive mouthparts were probably used to feed on nectar (Grimaldi and Barden, 2016; Zhang et al., 2016a). Eremochaetidae is probably related to the superfamily Archisargoidae based on the morphological characteristics (Grimaldi and Barden, 2016). The fossil record of eremochaetids reveals that the extinction of these ancient parasitoids probably occurred by the end of the Late Cretaceous and coincided with the rise of angiosperms, perhaps owing to competition from newly evolved parasitoid wasps and flies which extant ones are mostly flower-visiting insects (Eggleton and Belshaw, 1993; Feener and Brown, 1997; Gilbert and Jervis, 1998; Zhang et al., 2016a).

The Zhangsolvidae is an extinct family of brachyceran flies that erected by Nagatomi and Yang (1998) for the genus Zhangsolva cupressa found in the Early Cretaceous Laiyang Formation (Figure 1D; Zhang et al., 1993; Nagatomi and Yang, 1998; Arillo et al., 2015). Zhangsolvidae is a quite rare family that till now six species in four genera that found only in Cretaceous: five species and three genera in Early Cretaceous of China, Spain, Brazil and one species and genus in Late Cretaceous Burmese amber (Zhang et al., 1993; Nagatomi and Yang, 1998; Mazzarolo and Amorim, 2000; Wilkommen and Grimaldi, 2007; Arillo et al., 2015). Zhangsolvidae has a stout body, with very long and quite slender proboscis, vein M1 strongly arched, M3 fused to M4 and CuA fused to CuP (Nagatomi and Yang, 1998; Arillo et al., 2015). The placement of Zhangsolvidae is within Stratiomyomorpha supported by the presentation of phylogenetic analysis of 52 morphological characters for 35 taxa (Arillo et al., 2015). New zhangsolvids specimens from Early Cretaceous Spanish amber and mid-Cretaceous Burmese amber provided a detailed structure of their unique proboscis. Surprisingly, a specimen in Spanish amber is carrying clumped pollen that is attributed to a Mesozoic gymnosperm (Peñalver et al., 2015). The co-occurrence of pollen with its insect vector conforms that these long-proboscid insects were gymnosperm pollinators. Zhangsolvids became extinct during the late Cretaceous probably due to the extinction of their gymnosperm food.