AUTHOR=Li Ziyang , Wei Mengli , Zhong Yaping , Chong Yiyuan , Li Minghui 
  
TITLE=Characteristics of energy metabolism and stress load in elite MOBA E-sports athletes
  
JOURNAL=Frontiers in Physiology
  
VOLUME=Volume 17 - 2026
  
YEAR=2026
  
URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2026.1716237
  
DOI=10.3389/fphys.2026.1716237
  
ISSN=1664-042X
  
ABSTRACT=BackgroundE-sports gained growing recognition as a competitive pursuit with quantifiable physiological demands. While previous studies have shown that cognitive stress modulates energy metabolism and autonomic nervous system regulation, phase-specific physiological responses during gameplay remain poorly understood.Methods20 elite League of Legends players (rank ≥ Platinum) were enrolled. Their cardiopulmonary function and autonomic nervous system activity were monitored during rest and across three distinct match phases (early-, mid-, and late-game). Energy metabolism parameters were measured using a portable cardiopulmonary testing system, and heart rate variability indices were assessed with a chest-worn monitor.ResultsCompared with the resting state, the early-game elicited significant increases in energy expenditure oxygen consumption (VO2), carbon dioxide production (VCO2), and respiratory exchange ratio (all p < 0.001), with carbohydrate oxidation accounting for approximately 63% of total energy supply. Heart rate (HR) increased by 12.8%, while the root mean square of successive differences (RMSSD) rose by 52.2%, indicating sympathetic-parasympathetic coactivation. In the mid-game, metabolic indices declined but remained above baseline levels, characterized by sustained carbohydrate dominance (about 63.6%) and increased fat oxidation (about 30.2%); heart rate variability indices reflected sympathetic predominance accompanied by partial parasympathetic recovery. The late-game was characterized by slight rebounds in metabolic load and carbohydrate utilization (about 68.2%), accompanied by decreased Heart rate and elevated RMSSD, suggesting partial autonomic recovery alongside incipient neural fatigue.ConclusionElite e-sports athletes demonstrate dynamic, phase-dependent alterations in energy metabolism and autonomic nervous system regulation. The early phase is characterized by carbohydrate-dominated physiological activation, the mid phase by metabolic stabilization amid sustained cognitive demand, and the late phase by partial autonomic recovery with cumulative neural fatigue. These findings highlight the physiological mechanisms underlying E-sports performance and provide actionable insights for optimizing training regimens, fatigue monitoring protocols, and recovery interventions.