Front. Pharmacol.Frontiers in PharmacologyFront. Pharmacol.1663-9812Frontiers Media S.A.163220510.3389/fphar.2025.1632205PharmacologyCorrectionCorrigendum: Paricalcitol alleviates intestinal ischemia-reperfusion injury via inhibition of the ATF4-CHOP pathwayZhang et al.10.3389/fphar.2025.1632205ZhangJiawei1LiuTingting2XueTongqing3*JiaZhongzhi1*1Department of Interventional and Vascular Surgery, The Third Affiliated Hospital of Nanjing Medical University (Changzhou Second People’s Hospital), Changzhou, China2Graduate College of Dalian Medical University, Dalian, China3Department of Interventional Radiology, Huaian Hospital of Huai’an City (Huaian Cancer Hospital), Huai’an, China
Edited and reviewed by:Daniele Maria-Ferreira, Instituto de Pesquisa Pelé Pequeno Príncipe, Brazil
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A Corrigendum on Paricalcitol alleviates intestinal ischemia-reperfusion injury via inhibition of the ATF4-CHOP pathway by Zhang J, Liu T, Xue T, Jia Z (2025). Front Pharmacol. 16:1529343. doi: 10.3389/fphar.2025.1529343intestinalischemia reperfusion injuryparicalcitolATF4CHOPVDR (vitamin D receptor)section-at-acceptanceGastrointestinal and Hepatic Pharmacology
In the published article, there were errors in Figures 5C, 6C. Due to Authors’ oversight during figure preparation, the ATF4 and β-actin image that was originally intended for Figure 2C was mistakenly placed again in Figure 5C. Additionally, in Figure 6C, the TUNEL and DAPI staining images for the siATF4 group were incorrectly inserted. The authors also replaced the ATF4 and β-actin images in Figure 6, as the originally selected images were not sufficiently representative of the observed trends. The corrected Figures 5, 6 and its caption appear below.
Paricalcitol mitigates hypoxia-reoxygenation (H/R) injury in IEC-6 cells (A) Representative TUNEL staining (green) and nuclear staining (blue) results and apoptosis analysis of IEC-6 cells (scale bar = 100 μm; n = 6 in each group). (B) Differences in IEC-6 cell viability among groups assessed using the cell counting kit-8 assay (n = 6 in each group). (C) Western blot analysis and densitometric quantification of vitamin D receptor (VDR), activating transcription factor 4 (ATF4), and C/EBP homologous protein (CHOP) expression levels in IEC-6 cells (n = 6 in each group). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. All data are presented as mean ± SD. *, P values <0.05; **, P values <0.01; ***, P values <0.001; ****, P values <0.0001.
Fluorescence microscopy and bar graphs illustrate the impact of treatments on cells. Panel A shows TUNEL assay results highlighting apoptosis in different conditions: Control, Pari, H/R, and H/R+Pari. TUNEL and DAPI stains are displayed. The bar graph shows the TUNEL positive rate percentage. Panel B depicts cell viability and protein expression levels of VDR, ATF4, and CHOP, with significant differences indicated. Panel C presents Western blot analyses of VDR, ATF4, CHOP, and β-actin under the same conditions, supported by corresponding bar graphs of protein expression levels. Statistical significance is marked with asterisks.
Silencing vitamin D receptor (VDR) abolished the protective effect of paricalcitol in IEC-6 cells (A)The effect of siVDR treatment on cell viability assessed using cell counting kit-8 assay in IEC-6 cells (n = 6 in each group). (B) Western blot analysis and densitometric quantification of VDR, ATF4, and CHOP expression levels in IEC-6 cells after siVDR treatment (n = 6 in each group). (C) Representative TUNEL staining (green) and nuclear staining (blue) results for IEC-6 cells after siVDR treatment (scale bar = 200 μm; n = 6 in each group). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. All data are presented as mean ± SD. *, P values <0.05; **, P values <0.01; ***, P values <0.001; ****, P values <0.0001.
Graphs and images depict a study on cell viability and protein expression. Panel A shows a bar graph of cell viability across different conditions with significance indicated. Panel B presents Western blots for VDR, ATF4, CHOP, and β-actin with corresponding bar charts for protein expression levels. Panel C includes TUNEL assay images under different treatments, with green TUNEL-stained cells, blue DAPI-stained nuclei, and merged images alongside a bar graph showing TUNEL positive rates. Statistical significance is marked with asterisks and "ns" for not significant.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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