AUTHOR=Fang Jinpeng , Xu Mengtao , Qiu ZhengYue , Ye Tian , Jiang HongLing , Qin FengYi , Hu Yuan , Jiang Faying , Zhong JuHua , Zhu Yishuo , Zhao Lewei , Liu Xiubin , Zeng Jianguo , Xu Yuqin , Qing Zhixing 

TITLE=Lactation-promoting ingredients of Hemerocallis citrina Borani and the corresponding mechanisms

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1431856

DOI=10.3389/fphar.2024.1431856

ISSN=1663-9812

ABSTRACT=Hemerocallis citrina Borani was a traditional folk food used to promote the lactation of postpartum mothers in China, however, the active ingredients and corresponding mechanisms are still unknown. In this study, The lactogenic effect of alcoholic and aqueous extracts of H. citrina was primarily evaluated, and the aqueous extract (1000 and 2000 mg/kg) displayed significant lactationpromoting effects. Three eluates of aqueous extract (0%, 30%, and 50%HCW) further evaluated their lactogenic effect, and 30% and 50%HCW showed significant lactation-promoting activity. Nineteen ingredients, including high-content of rutin and isoquercetin, were then identified from 30% and  50%HCW by the ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS) method. Finally, the lactogenic effect of rutin and isoquercetin were evaluated, and both compounds displayed significant lactation-promoting activity. The mechanisms relative to the lactation-promoting active for H. citrina extracts and compounds are to stimulate the release of prolactin (PRL) and progesterone (P), as well as to induce the expression of prolactin receptor (PRLR) and improve the morphology of mammary tissue. This study first clarified the lactationpromoting active ingredients of H. citrina and the corresponding mechanisms, which provide new insight into the new lactation-promoting drug and promote the high-value utilization of H. citrina resources.