Front. Pharmacol. Frontiers in Pharmacology Front. Pharmacol. 1663-9812 Frontiers Media S.A. 1108989 10.3389/fphar.2022.1108989 Pharmacology Correction Addendum: A novel N-Arylpyridone compound alleviates the inflammatory and fibrotic reaction of silicosis by inhibiting the ASK1-p38 pathway and regulating macrophage polarization Fan et al. 10.3389/fphar.2022.1108989 Fan Mingming 1 2 Xiao Huijuan 2 3 Song Dingyun 2 Zhu Lili 2 Zhang Jie 1 Zhang Xinran 2 4 Wang Jing 5 * Dai Huaping 2 * Wang Chen 1 2 5 * 1 Department of Respiratory Medicine, The Second Hospital of Jilin University, Jilin, China 2 National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Department of Pulmonary and Critical Care Medicine Center of Respiratory Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, China-Japan Friendship Hospital, Capital Medical University, Institute of Respiratory Medicine, Beijing, China 3 Department of Pulmonary and Critical Care Medicine, China-Japan Friendship School of Clinical Medicine, Peking University, Beijing, China 4 Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China 5 State Key Laboratory of Medical Molecular Biology, School of Basic Medicine Peking Union Medical College, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, Beijing, China

Edited and reviewed by: Paola Patrignani, University of Studies G. d'Annunzio Chieti and Pescara, Italy

 *Correspondence: Jing Wang, wangjing@ibms.pumc.edu.cn; Huaping Dai, daihuaping@ccmu.edu.cn; Chen Wang, wangchen@pumc.edu.cn

This article was submitted to Inflammation Pharmacology, a section of the journal Frontiers in Pharmacology

 18 01 2023 2022 13 1108989 27 11 2022 29 11 2022 Copyright © 2023 Fan, Xiao, Song, Zhu, Zhang, Zhang, Wang, Dai and Wang. 2023 Fan, Xiao, Song, Zhu, Zhang, Zhang, Wang, Dai and Wang

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 An Addendum on A Novel N-Arylpyridone compound alleviates the inflammatory and fibrotic reaction of silicosis by inhibiting the ASK1-p38 pathway and regulating macrophage polarization by Fan M, Xiao H, Song D, Zhu L, Zhang J, Zhang X, Wang J, Dai H and Wang C (2022). Front. Pharmacol. 13:848435. doi: 10.3389/fphar.2022.848435 AKEX0011 macrophage polarization pirfenidone pulmonary fibrosis silicosis Missing information

Add information about chemical formula, Table 2, polarization and antibody list.

(Antibody list) Primary antibodies for Western Blot.

Antibodies Cat No. Manufacturer Sources of species
Fibronectin ab2413 abcam Rabbit
Collagen-Ⅰ 66761-1-Ig proteintech Mouse
IκBα #4814 cell signaling technology Mouse
p38 #8690 cell signaling technology Rabbit
P-p38 #4511 cell signaling technology Rabbit
Arginase-1 ab233548 abcam Rabbit
ASK-1 67072-1-Ig proteintech Rabbit
P-ASK-1 28846-1-AP proteintech Rabbit
NF-kB ab32536 abcam Rabbit
P-NF-kB ab76302 abcam Rabbit
iNOS 18985-1-AP proteintech Rabbit
β-Actin ab8226 abcam Mouse
Chemical formula

Chemical structure of AKEX0011

Molecular formula C18H14N5O2F3, mass 389.3 g/mol, CAS:1590403-33-0.

Polarization

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This macrophage cell line is commonly used to study phagocytosis, apoptosis, inflammation, as well as M1/M2 polarization. Polarization of M1/M2 phenotype can be determined by the expression of specific M1 (CD80, CD86) and M2 (CD206, CD163) markers detected by flow cytometry. In the in vitro experiments of our study, we detected the expression of CD86 and CD163 in RAW264.7 cells of each group by flow cytometry to explore macrophage polarization. Our flow cytometry results confirmed that silica induced polarization of RAW264.7 from M0 macrophages toward certain M1 subtype (F4/80 + CD86+), but have no effect on the polarization towards M2 subtype (F4/80 + CD163+).

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