A cationic antibiotic Bogorol A (1) (Figure S1), displaying potent activity against both methicillin-resistant Staphylococcus aureus (MRSA, MIC 2 μg/mL) and vancomycin-resistant enterococcal strains (VRE, MIC 10 μg/mL) of bacteria, has been isolated from cultures of a marine Bacillus laterosporus collected in Papua New Guinea (Barsby et al., 2001). A new broad spectrum thiazolyl peptide antibiotic, Nocathiacins I–III (2–4) (Figure S1), was isolated from the cultured broth of Nocardia sp. They share structural similarities to glycothiohexide-alpha (Li et al., 2003). All compounds exhibit potent in vitro activity against several multiple-drug resistant pathogens with MIC of 0.001–0.015, 0.0005–0.25, 0.002–0.06 μg/mL respectively. They demonstrate excellent in vivo efficacy in a systemic S. aureus infection mouse model at PD₅₀ 0.8, 0.6, 0.6 mg/kg/day respectively (Leet et al., 2003). The marine sponge Halichondria japonica was the source of Bacillus cereus which gave two cyclic thiopeptide antibiotics, YM-266183 (5) and YM-266184 (6) (Figure S1). They exhibited potent antibacterial activity against Staphylococci (MIC 0.05–0.2 μg/mL, 0.013–0.025 μg/mL) and Enterococci including multiple drug resistant strains (MIC 0.02–0.05 μg/mL, 0.006–0.01 μg/mL), whereas, they were inactive against gram-negative bacteria (Nagai et al., 2003). These structures contain thiazole and pyridine moieties and several unusual amino acids (Suzumura et al., 2003). A bacterial Ruegeria species isolated from a sponge Suberites domuncula (Gulf of Naples, Italy), gave two new cyclic peptides, cyclo-(glycyl-l-seryl-l-prolyl-l- glutamyl) (7) and cyclo-(glycyl-l-prolyl-l-glutamyl) (8) (Figure S1) with moderate antimicrobial activity against Bacillus subtilis at MIC of 25 and 50 μg/mL, respectively (Mitova et al., 2004). B. laterosporus PNG276 obtained from Papua New Guinea was the source of a new lipopeptide antibiotic, Tauramamide (9) (Figure S1). Anti-pathogenic activity against Enterococcus sp. were reported for tauramamide and its ethyl ester at MIC 0.1 μg/mL (Desjardine et al., 2007). Pseudomonas sp. separated out from the seaweed Diginea sp. (Ishigaki Is., Okinawa, Japan) were the source of cyclic tetrapeptides cyclo-[phenylalanyl-prolyl-leucyl-prolyl] (10) and cyclo-[isoleucyl-prolyl-leucyl-alanyl] (11) (Figure S1). The crude extract of this bacterial culture was found to inhibit the growth of other marine bacterial strains (Rungprom et al., 2008).

Unnarmicin A (12) and C (13) (Figure S2) are two depsipeptides isolated from a culture of a marine bacterium, Photobacterium sp. strain MBIC06485 having selective inhibitory effect on Pseudovibrio bacterial strains (Oku et al., 2008b). A strong antibacterial thiopeptide antibiotic TP-1161 (14) (Figure S2) with a rare aminoacetone moiety, have been isolated from Nocardiopsis sp. MICs value of TP-1161, ranging from 0.25 to 4 μg/ml for most gram-positive strain. The gene cluster for the biosynthesis of (14) was identified by PCR screening using degenerate primers (Engelhardt et al., 2010). Marine Photobacterium halotolerant yielded two novel cyclodepsipeptides Solonamide A (15) and B (16) (Figure S2) with inhibitory effect on virulence gene expression in S. aureus (Mansson et al., 2011). The fermentation broth of Streptomyces strain isolated from a marine sediment sample collected off Nasese, Fiji were the source of three new depsipeptides, Fijimycins A–C (17–19) (Figure S2). Fijimycins A–C were shown significant activity against three MRSA strains with MIC₁₀₀ values between 4 and 16 μg/mL (Sun et al., 2011). Peptidolipins B–F (20–24) (Figure S2), antibacterial lipopeptides were obtained from an ascidian-derived Nocardia sp. Peptide (20) and (23) were moderately antibacterial against MRSA and methicillin-sensitive S. aureus (MSSA) (Wyche et al., 2012). A marine-derived bacterium Kocuria palustris was the source of a new thiazolyl peptide, Kocurin (25) (Figure S2). Kocurin strongly inhibits MRSA MB5393 with a MIC value of 0.25 μg/mL (Martín et al., 2013). An octapeptide, Champacyclin (26) (Figure S2) was isolated from three strains of Streptomyces champavatii (sediment, Gotland Deep and Kiel Bight, Baltic Sea and Urania Basin, Eastern Mediterranean) as an inhibitor of blight disease causing bacterium Erwinia amylovora (Pesic et al., 2013). Cyclodepsipeptides Ngercheumicin F–I (27–30) (Figure S2) isolated from P. halotolerans, inhibited quorum sensing in S. aureus (Kjaerulff et al., 2013).

Lobocyclamide B (31) (Figure S3) a cyclododecapeptide containing five beta-hydroxy-alpha-amino acid residues, was discovered from Lyngbya confervoides which was active against fluconazole-resistant C. albicans. The absolute stereochemistry was determined by chiral chromatography of Marfey's reaction (MacMillan and Molinski, 2002). Brunsvicamides A–C (32–34) (Figure S3), three new cyclic hexapeptides have been isolated from cyanobacterium Tychonema sp. Brunsvicamide C contains an N-methylated N'-formylkynurenine moiety. Brunsvicamide B selectively inhibits the Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB, IC₅₀ 7.3 μM) (Müller et al., 2006).

An extraction of a saltwater culture of an unidentifiable sponge-derived fungus leads to discovering two novel cyclic depsipeptides, Guangomides A (35) and B (36) (Figure S4). Both compounds had weak antibacterial activity against Staphylococcus epidermis (MIC = 100 μg/mL, each) and Enterococcus durans (MIC = 100 μg/mL, each) (Amagata et al., 2006). A marine-derived Aspergillus fumigatus yielded to 11-O-methylpseurotin A (37) (Figure S4) (PKS/NRPS), which selectively inhibited a Hof1 deletion strain of the yeast Saccharomyces cerevisiae (Boot et al., 2007). Marine-derived fungus Emericella sp., and marine actinomycete Salinispora arenicola were co-cultured to induce production of Emericellamides A (38) and B (39) (Figure S4) by fungi. Emericellamides A and B displayed modest antibacterial activities against MRSA with MIC values of 3.8 and 6.0 μM, respectively (Oh et al., 2007).

The fungus Scopulariopsis brevicaulis, isolated from marine sponge Tethya aurantium was the source of two novel cyclodepsipeptides, Scopularides A (40) and B (41) (Figure S4), which were weak inhibitors of gram-positive bacteria (Yu et al., 2008). Alternaramide (42) (Figure S4), a cyclic Penta depsipeptide, was produced by culture of Alternaria sp. which was isolated from sediment, Masan Bay, S. Korea. It's showed weak antimicrobial activity against B. subtilis (ZOI 8 mm) and S. aureus (ZOI 13 mm) (Kim et al., 2009). Trichoderins A (43), A1 (44), and B (45) (Figure S4) are three new amino lipopeptides reported from marine sponge-derived fungus of Trichoderma sp. All trichoderins have shown potent anti-mycobacterial activity against Mycobacterium smegmatis, Mycobacterium bovis BCG, and M. tuberculosis H37Rv under aerobic and dormancy-inducing hypoxic growth conditions with MIC values in the range of 0.02–2.0 l g/mL (Pruksakorn et al., 2010).

Aspergillus sp. AF119 was the source of γ-aminobutyric acid containing cyclic heptapeptide Unguisin E (46) (Figure S4) (Liu and Shen, 2011). Marine-derived halotolerant Aspergillus sclerotiorum PT06-1 gave to two novel cyclic hexapeptides Sclerotides A (47) and B (48) (Figure S4) in a nutrient-limited hypersaline medium. Both of these peptides were photo inter convertible, containing anthranilic acid, dehydroamino acid units and showed moderate antifungal activity against C. albicans. Compound (47) also inhibited P. aeruginosa growth (Zheng et al., 2009). A halotolerant A. sclerotiorum PT06-1 isolated from salt sediments from the Putian Sea Salt Field, Fujian, China was the source of 11 new aspochracin-type cyclic tripeptides, Sclerotiotides A–K (49–59) (Figure S4). Only sclerotiotides A, B, F and I showed selective antifungal activity against C. albicans with MIC values of 7.5, 3.8, 30, and 6.7 μM, respectively (Zheng et al., 2010).

Three new cytotoxic cyclopeptides, Mixirins A–C (60–62) (Figure S5) belonging to iturin class have been isolated from marine bacterium Bacillus sp. obtained from the mud near the Arctic pole. All compounds inhibited the growth of human colon tumor cells (HCT-116) with IC₅₀ of 0.68, 1.6, 1.3 μg/ml. (Zhang et al., 2004). A Thermoactinomyces specie YM3-251 have been isolated from mud (Mecherchar, Republic of Palau), which was the source of a cyclic peptide Mechercharmycin A (63) as well as the linear congener Mechercharmycin B (64) (Figure S5). Mechercharmycin A exhibited relatively strong antitumor activity against A549 cells (human lung cancer) and Jurkat cells (human leukemia) with IC₅₀ value of 4.0 × 10⁻⁸ M and 4.6 × 10⁻⁸ M respectively, whereas mechercharmycin B exhibited no activity (Kanoh et al., 2005). Bromoalterochromides A and A' (65–66) (Figure S5), an unprecedented chromo peptide was produced by a marine Pseudoalteromonas maricaloris strain KMM 636 which was isolated from sponge Fascaplysinopsis reticulata. Chemically both of these compounds are brominated yellow colored Thr-Val-Asn-Asn-X pentapeptide lactones, where the amino group of Thr is acylated with 9-(3-bromo-4-hydroxyphenyl)-nona-2, 4,6,8-tetraenoic acid, and X is aIle and Leu, respectively. They showed moderate cytotoxic effects on developing eggs of the sea urchin Strongylocentrotus intermedius (Speitling et al., 2007). New 3-methyl-4-ethylideneproline-containing cytotoxic peptides, Lucentamycins A–D (67–70) (Figures S5, S6) have been isolated from the broth of a marine-derived actinomycete Nocardiopsis lucentensis (strain CNR-712). Only lucentamycins A and B showed significant in vitro cytotoxicity against HCT-116 human colon carcinoma with IC₅₀ values of 0.20 and 11 μM, respectively (Cho et al., 2007).

Three cyclic hexadepsipeptides Piperazimycins A–C (71–73) (Figure S6) have been isolated from the fermentation broth of a Streptomyces sp. (sediment, Guam). The structures of these cyclic hexadepsipeptides have shown presence of rare amino acids, including hydroxyacetic acid, α-methylserine, γ-hydroxypiperazic acid, γ-chloropiperazic acid 2-amino-8-methyl-4, 6-nonadienoic acid, and 2-amino-8-methyl-4,6-decadienoic acid and were all significantly cytotoxic against multiple tumor cell lines with an average GI₅₀ 76 ng/mL for each (Miller et al., 2007). The cultured mycelia of marine bacterium Mechercharimyces asporophorigenens (marine lake sediment, Urukthapel Island, Palau) was the source of Urukthapelstatin A (74) (Figure S6), a cyclic thiopeptide that displayed potent activity against a human cancer cell line panel. Urukthapelstatin A has also shown growth inhibition of human lung cancer A549 cells in dose-dependent manner with an IC value of 12 nM (Matsuo et al., 2007). The culture of Salinispora arenicola isolated from sea sediment (Great Astrolabe Reef, Fijiy) yielded three new cyclohexadepsipeptides, Arenamides A–C (75–77) (Figure S6). The absolute structures and configuration of these compounds were established by the spectroscopic technique. Arenamides A (75) and B (76) blocked tumor necrosis factor (TNF)-induced activation with IC₅₀ values of 3.7 and 1.7 μM respectively. In addition, they also inhibited nitric oxide and prostaglandin E2 production and were moderately cytotoxic to HCT-116 cells (Asolkar et al., 2008). Bacillus silvestris that was isolated from a Pacific Ocean (southern Chile) crab yields two new cyclodepsipeptides, Bacillistatins 1-2 (78–79) (Figure S6) with strong anti-cancer (GI₅₀ of 10⁻⁴-10⁻⁵ μg/mL) activity (Pettit et al., 2009). The epimeric cyclic peptides Turnagainolides A (80) and B (81) (Figure S6), isolated from marine Bacillus sp. (sediment, Turnagain Is., British Columbia, Canada), had indirect inhibitory effect on phosphatidylinositol-3-kinase (PI3K) pathway (Li et al., 2011). A Streptomyces sp. obtained from marine sediment produced two highly modified linear tetrapeptides, Padanamides A (82), and B (83) (Figure S6). They inhibit cysteine and methionine biosynthesis and are cytotoxic to Jurkat cells (IC₅₀ of 20 μg/mL) respectively (Williams et al., 2011). Chemical genomics was performed to discover the mode of action of compounds, which suggested that padanamide A inhibits cysteine and methionine biosynthesis.

Streptomyces sp. isolated from volcanic island produced new cyclic peptides Ohmyungsamycin A (84) and B (85) (Figure S7). The presence of unusual amino acid units, including N-methyl-4-methoxytrytophan, β-hydroxyphenylalanine, and N, N-dimethylvaline in compound (84–85) have been determined by interpretation of the NMR, UV, and IR spectroscopic and MS data. Both exhibited inhibitory activities against diverse cancer cells with IC₅₀ values ranging from 359 to 816 nM and 12.4 to 16.8 μM respectively. However, compound (84) was more active in this regard interestingly; these compounds exhibit relatively selective anti-proliferative activity against cancer cells compared to normal cells. This may be due to the consequence of genetic background or of the biologically various characteristics between cancer and normal cells. However, the exact molecular mechanism behind the selectivity should be further investigated (Um et al., 2013). Proximicins A–C (86–88) (Figure S7) are novel aminofuran antibiotics with anticancer activity, isolated from marine strains of verrucosispora sp. Compounds (86–88) showed inhibitory activity against gastric adenocarcinoma (AGS, IG₅₀ = 0.6, 1.5, 0.25 μg/mL respectively), hepatocellular carcinoma (HepG2, IG₅₀ = 0.8, 9.5, 0.7 μg/mL respectively) and breast carcinoma cells (MCF 7, IG₅₀ = 7.2, 5.0, 9.0 μg/mL respectively). A cell-cycle analysis in AGS cells revealed that Proximicin C produced cell arrest in the G0/G1 phase after incubation for 24 h. After 40 h, there was an increase in the number of cells in the sub-G1 phase, that is, apoptotic cells (+2.9%). It was also found that proximicin C induce upregulation of p53 and of the cyclin kinase inhibitor p21 in AGS cells (Fiedler et al., 2008).

An assemblage of Lyngbya majuscula and Phormidium gracile collected in Papua New Guinea produced a cyclic depsipeptide Hoiamide A (89) (Figure S8). The highly unusual structure of hoiamide A synthesized by mixed peptide–polyketide biosynthetic pathway showed moderate cytotoxicity to cancer cells and partial agonist of site 2 on the voltage-gated sodium channel as it produced a rapid and concentration-dependent elevation of neuronal [Na⁺] in neocortical neurons (IC₅₀ = 92.8 nM) (Pereira et al., 2009). An assemblage of the marine cyanobacteria L. majuscula and Schizothrix species collected from Fiji was the source of cyclic depsipeptides Yanucamides A (90) and B (91) (Figure S8), which contain a 2, 2-dimethyl-3-hydroxyoct-7-ynoic acid moiety. Both compounds exhibited strong brine shrimp toxicity (LD₅₀, 5 ppm) (Sitachitta et al., 2000). The cyclic depsipeptides named Lyngbyabellins A (92) (Figure S8), contain a 7,7-dichloro-2,2-dimethyl-3-hydroxyoctanoic acid moiety have been isolated from the cytotoxic fraction of L. majuscula collected from Guam and the Dry Tortugas National Park, Florida. Compound (92) have moderate cytotoxicity against human nasopharyngeal carcinoma cell line (KB cells) and human colon adenocarcinoma cell line (LoVo cells), with IC₅₀ values of 0.03 and 0.50 μg/mL, respectively and also showed cellular microfilament network in A-10 cells at 0.01–5.0 μg/mL concentrations (Luesch et al., 2000). Another collection from Tortugas National Park, Florida was the source of cytotoxic and antifungal cyclic depsipeptide Lyngbyabellin B (93) (Figure S8). Lyngbyabellin B was toxic to brine shrimp (LD₅₀ = 3.0 ppm) (Milligan et al., 2000). A marine cyanobacterium Microcystis aeruginosa contained the cyclic hexapeptide Microcyclamide (94) (Figure S8), which showed moderate cytotoxicity against P388 murine leukemia cells at 24–30 μg/mL (Ishida et al., 2000).

The cyanobacterium L. majuscule collected from Guam was the source of Apratoxin A (95) (Figure S8). This cyclodepsipeptide of mixed peptide-polyketide biogenesis exhibited in vitro cytotoxicity against human tumor cell lines at IC₅₀ of 0.36–0.52 nM. Apratoxin A induces G1 phase cell arrest and apoptosis, which is at least particularly initiated through antagonism of FGF signaling via STAT3 (Luesch et al., 2001b). Another collection of L. majuscule from Guam gave two cyclic depsipeptides, Pitipeptolides A (96) and B (97) (Figure S8) with anti-mycobacterial and weak cytotoxicity against LoVo cells with IC₅₀ values of 2.25 and 1.95 μg/mL, respectively. Pitipeptolides A and B also stimulated elastase activity. It is suggested that this activity is due to the presence of hydrophobic portions in the molecule (Luesch et al., 2001a). Marine cyanobacterium Lyngbya sp. collected from Palauan was the source of six new β-amino acid-containing cyclic depsipeptides, the Ulongamides A–F (98–103) (Figure S8). All peptides were found to be weakly cytotoxic against KB and LoVo cells with IC₅₀ values of ca. 1 μM and ca. 5 μM respectively except compound Ulongamides F (Luesch et al., 2002). Examination of a L. confervoides collection from Saipan, Commonwealth of the Northern Mariana Islands, led to the isolation of a novel cytotoxic cyclic depsipeptide Obyanamide (104) (Figure S8). Obyanamide was cytotoxic against KB cells with an IC₅₀ of 0.58 μg/mL. According to the results, the β-amino acid residue was found to play a critical role in the biological activities. Additionally, the ester bond along with the Ala (Thz) moiety was also essential for biological activities (Williams et al., 2002a). Malevamide D (105) (Figure S8), a highly cytotoxic peptide ester have been isolated from marine cyanobacterium Symploca hydnoides (Horgen et al., 2002). A culture Symploca sp. yielded Tasiamide (106) (Figure S8), an acyclic peptide. Tasiamide demonstrated cytotoxic activity against KB and LoVo cells with IC₅₀ values of 0.48 and 3.47 μg/mL, respectively (Williams et al., 2002b). A new cytotoxic peptide Tasiamide B (107) (Figure S9) which contain the unusual amino acid-derived residue 4-amino-3-hydroxy-5-phenylpentanoic acid (Ahppa) have been isolated from cyanobacterium Symploca sp. This peptide displayed an IC₅₀ value of 0.8 μM against KB cells (Williams et al., 2003b).

A Papua New Guinea collection of the marine cyanobacterium L. majuscule was the source of six cyclic depsipeptides, Guineamides A–F (108–113) (Figure S9). The presence of beta-amino or beta-hydroxy carboxylic acid residues in all peptides was determined using a combination of chemical manipulations as well as Marfey's method. Guineamides B and C showed moderate cytotoxicty to a mouse neuroblastoma cell line with IC₅₀ values of 15 and 16 μM, respectively (Tan et al., 2003b). A new bioactive cyclic depsipeptide, Homodolastatin 16 (114) (Figure S9) have been isolated from L. majuscula, collected from Wasini Island off the southern Kenyan coast. Homodolastatin 16 showed moderate activity against oesophageal (IC₅₀ = 4.3 μg/mL) and cervical cancer cell lines (IC₅₀ = 1 μg/mL) (Davies-Coleman et al., 2003). An examination of an organic extract of a cyanobacterium L. majuscula, collected from Guam, led to the isolation of the cyclic peptide Lyngbyastatin 3 (115) (Figure S9). The presence of two unusual amino acid units, 3-amino-2-methylhexanoic acid (Amha) and 4-amino-2, 2-dimethyl-3-oxopentanoic acid units (Ibu) was determined by standard methods. Lyngbyastatin 3 displayed in vitro activity against KB and LoVo cell lines with IC₅₀ values of 32 and 400 nM respectively (Williams et al., 2003a).

A collection of Lyngbya sp. from Palauan was the source of cytotoxic cyclic depsipeptide Ulongapeptin (116) (Figure S9) with an IC₅₀ value of 0.63 μM against KB cells (Williams et al., 2003d). Two new depsipeptides Tasipeptins A (117) and B (118) (Figure S9) have been isolated from Palau collection of Symploca sp. The gross structure of all peptides (117–118) were determined by standard methods and was found to contain unusual amino acid-derived residue 4-amino-3-hydroxy-5-phenylpentanoic acid (Ahppa) and 3-amino-6-hydroxy-2-piperidone (Ahp) moiety respectively. Both were cytotoxic toward KB cells with IC₅₀ values of 0.93 and 0.82 μM, respectively (Williams et al., 2003c). Lyngbya majuscula collected from Hector's Bay, Jamaica was found to contain three lipopeptides, Jamaicamides A–C (119–121) (Figure S9). Further biological investigation of the jamaicamides has revealed that they are generated by iterative hybrid PKS-NRPS assembly and exhibited cytotoxicity to both the H-460 human lung and Neuro-2a mouse neuroblastoma cell lines (IC₅₀ = 15 μM for all), sodium channel blocking activity at 5 μM and ichthyotoxic activities (Edwards et al., 2004). Four new depsipeptides, Wewakpeptins A–D (122–125) (Figures S9, S10), were found cytotoxic to brine shrimp and to the NCI-H460 and neuro-2a cell lines (LC₅₀ of approximately 0.4 μM). These were isolated from Lyngbya semiplena collected from Wewak Bay, Papua New Guinea (Han et al., 2005).

Trungapeptins A-C (126–128) (Figure S10), cyclodepsipeptides have been isolated from marine cyanobacterium L. majuscula. Trungapeptin A exhibited mild icthyotoxicity (6.25 ppm) and weak toxicity to brine shrimp (10 ppm) (Bunyajetpong et al., 2006). Cytotoxic cyclic peptides, Aurilides B (129) and C (130) (Figure S10) were produced by marine cyanobacterium L. majuscula collected from Papua New Guinea. Both aurilides B and C described to induce a dysfunction in mitochondria in NCI-H460 human lung tumor and the neuro-2a mouse neuroblastoma cell lines, with LC₅₀ values between 0.01 and 0.13 μM (Han et al., 2006). A highly methylated tetrapeptide Belamide A (131) (Figure S10) was isolated from Symploca sp. (Salmedina Reef, Panama) which was shown antimitotic and cytotoxic to MCF7 breast cancer and HCT-116 cell lines (IC₅₀ 0.74 μM) by microtubule disruption with structural analogy to the important linear peptides dolastatins 10 and 15 (Simmons et al., 2006). Three new analogs of dolastatin 13, Lyngbyastatins 5-7 (132–134) (Figure S10), have been isolated from two different collections of marine cyanobacteria, Lyngbya sp., from South Florida with previously reported cyclodepsipeptide somamide B. Compounds (132–134) were found to selectively inhibit elastase over several other serine proteases, with IC₅₀ values for porcine pancreatic elastase ranging from 3 to 10 nM (Taori et al., 2007). A new linear peptide Mitsoamide (135) (Figure S10) was produced by marine cyanobacterium Geitlerinema sp. collected from Mitso-Ankaraha Island. Mitsoamide has shown strong activity against NCI-H460 human lung tumor cells with LC₅₀ of 460 nM (Andrianasolo et al., 2007).

A Papua New Guinea collection of the marine cyanobacteria L. majuscula and Lyngbya sordida was the source of potent cytotoxic cyclodepsipeptide Apratoxin D (136) (Figure S10). Compound (136) possesses 3, 7-dihydroxy-2, 5, 8, 10, 10-pentamethylundecanoic acid as the polyketide moiety and potent in vitro cytotoxicity against H-460 human lung cancer cells with an IC₅₀ value of 2.6 nM (Gutiérrez et al., 2008). Another collection of marine cyanobacterium Lyngbya bouillonii from a Guamanian was found to contain Apratoxin E (137) (Figure S10), which was strongly cytotoxic to several cancer cell lines at IC₅₀ 21–72 nM (Matthew et al., 2008). Dragonamides C (138) and D (139) (Figures S10, S11) are linear lipopeptides isolated from the marine cyanobacterium brown Lyngbya polychroa. Both peptides are weak cytotoxic against several cancer cell lines with GI₅₀ values of 56 and 59 μM against U2OS osteosarcoma cells, 22 and 32 μM against HT29 colon adenocarcinoma cells, and 49 and 51 μM against IMR-32 neuroblastoma cells, respectively (Gunasekera et al., 2008). Coibamide A (140) (Figure S11), a potent anti-proliferative highly methylated cyclic depsipeptide was isolated from a culture of Leptolyngbya sp. which was collected from the Coiba National Park, Panama. Compound (140) showed an unprecedented selectivity profile in the NCI 60 cancer cell line panel (LC₅₀ < 23 nM). It causes S phase inhibition in cell cycle (Medina et al., 2008). Symploca sp. yielded dolastatin 10/15 hybrid linear peptide Symplostatin 4 (141) (Figure S11) which was shown to be antimitotic activity via microtubule depolymerization to H-460 lung cancer cells (IC₅₀ = 40 nM) as well as neuro-2a neuroblastoma cells (IC₅₀ = 29 nM) (Taori et al., 2009).

A culture of L. majuscule obtained from True Blue Bay, eastern Caribbean yielded two new depsipeptides, Itralamides A (142) and B (143) (Figure S11). Only itralamide B was found to cytotoxic to HEK293 cells IC₅₀ 6 ± 1 μM (Jiménez et al., 2009). An active peptide metabolite Desmethoxymajusculamide C (144) (Figure S11) (DMMC) have been isolated from Fijian collection of L. majuscule. Both cyclic and liner version of DMMC were found potent and showed selective anti-solid tumor activity at IC₅₀ = 20 nM against HCT-116 through disruption of cellular microfilament networks (Simmons et al., 2009). A Singapore collection of L. majuscule has been shown to produce cyclodepsipeptide, Hantupeptin A (145) (Figure S11) with cytotoxicity to MOLT-4 leukemia cells (IC₅₀ 32 μM) and MCF-7 breast cancer cells (IC₅₀ 4.0 μM) (Tripathi et al., 2008). Chemical investigation of Lyngbya cf. polychroa resulted in isolation of a linear peptide desacetylmicrocolin B (146) (Figure S11), was a growth inhibitor of HT-29 (IC₅₀ 14 nM) and IMR-32 cells (IC₅₀ 14 nM) (Meickle et al., 2009).

Palmyramide A (147) (Figure S11), a cyclic depsipeptide found to block sodium channel in neuro-2a cells (IC₅₀ 17.2 μM) and modest cytotoxicity to H-460 cells (IC₅₀ 39.7 μM) and was isolated from from a Palmyra Atoll Collection of the marine cyanobacterium L. majuscule (Taniguchi et al., 2009). Lyngbya sp. was the source of a potent cytotoxic peptide Bisebromoamide (148) (Figure S11) which potentially inhibit protein kinase and is cytotoxic to HeLa S3 cells with an IC₅₀ value of 0.04 μg/mL (Teruya et al., 2009). An examination of an organic extract of the cyanobacterium L. bouillonii, collected from Guam, led to the isolation of cytoskeletal actin-disrupting and cytotoxic (IC₅₀ = 0.041 μM) Lyngbyabellin J (149) and a linear modified peptide, lyngbyapeptin D (150) (Figure S11) (Matthew et al., 2010). Marine cyanobacterium L. confervoides gave bis-thiazoline containing cyclic depsipeptides, Grassypeptolides A–C (151-153) (Figure S12). All grassypeptolides cause G1 phase cell cycle arrest in HT29 (IC₅₀ = 1.22, 4.97 μM, 76.7 nM) and HeLa cell lines (IC₅₀ = 1.01, 2.93 μM, 44.6 nM) (Kwan et al., 2010). Hantupeptins B (154) and C (155) (Figure S12), two cytotoxic cyclodepsipeptides were obtained from a marine cyanobacterium L. majuscule collected from Pulau Hantu Besar, Singapore. Compound (154) gave an IC₅₀ of 0.2 μM against MOLT-4 and 0.5 μM against MCF-7 cancer cell lines however compound (155) showed moderate cytotoxicity against the MOLT-4 and MCF-7 cancer cell lines with IC₅₀ values of 3.0 μM and 1.0 μM, respectively (Tripathi et al., 2010). Marine cyanobacteria Lyngbya sp. was the source of novel cyclic peptides Lyngbyacyclamides A (156) and B (157) (Figure S12) which moderately inhibited the growth of B16 mouse melanoma cells (IC₅₀ of 0.7 μM) (Maru et al., 2010).

The marine cyanobacterium Leptolyngbya sp. collected from the SS Thistlegorm shipwreck in the Red Sea offered two cyclic depsipeptides, Grassypeptolides D (158) and E (159) (Figure S12). Both of these peptides were cytotoxic to HeLa (IC₅₀ = 335 and 192 nM, respectively) and mouse neuro-2a blastoma cells (IC₅₀ = 599 and 407 nM, respectively) (Thornburg et al., 2011). Bis-thiazoline-containing cyclic depsipeptides Grassypeptolides F (160) and G (161) (Figure S12) which contains rare β-amino acid, extensive N-methylation and a large number of d-amino acids was isolated from an extract of Palauan cyanobacterium L. majuscule. Both (160) and (161) were found to have moderate inhibitory activity against the transcription factor AP-1 (IC₅₀ = 5.2 and 6.0 μM, respectively) (Popplewell et al., 2011). An examination of an organic extract of the cyanobacterium Symploca cf. hydnoides sampled from Cetti Bay, Guam, led to the isolation of the eleven new peptides Veraguamides A-G (162–168), Veraguamide H (169) and J–L (170–172) (Figure S12). Veraguamide A showed potent cytotoxicity to H-460 human lung cancer cell line at LD₅₀ = 141 nM whilst the others were weak inhibitors. Their structures were elucidated by combining various techniques in spectroscopy, chromatography, and synthetic chemistry (Mevers et al., 2011; Salvador et al., 2011).

Three new cyclic peptides Malyngamide 3 (173) and Cocosamides A (174) and B (175) (Figure S13) have been isolated from the lipophilic extract of marine cyanobacteria L. majuscula collected from Cocos Lagoon, Guam and were found to modestly cytotoxic to HT-29 cells with IC₅₀ value of 48, 24, and 11 μM respectively (Gunasekera et al., 2011). L. majuscula (Piti Bomb Holes, Guam) was the source of proline rich unusual cyclic depsipeptide Pitiprolamide (176) (Figure S13). Further investigation yielded four more peptides Pitipeptolides C–F (177–180) (Figure S13). All peptides were found moderately cytotoxic against two HTCLs, however, pitipeptolides C–F were more active against M. tuberculosis and B. cereus as compared to compound (176) (Montaser et al., 2010). The marine cyanobacterium L. majuscule collected from western lagoon of Pulau Hantu Besar, Singapore was the source of cyclodepsipeptide Lagunamide C (181) (Figure S13). Lagunamide C exhibited potent cytotoxic activity against HTCLs with IC₅₀ values ranging from 2.1 to 24.4 nM, antimalarial activity against Plasmodium falciparum (IC₅₀ 0.29 μM) and weak anti-swarming activity against P. aeruginosa (Tripathi et al., 2011).

A collection of marine cyanobacterium L. semiplena and L. majuscule from Papua New Guinea led to isolation of the cyclic depsipeptides Wewakamide A (182) and Guineamide G (183) (Figure S13) were respectively. Both of these peptides displayed potent toxicity against brine shrimp and only guineamide G showed cytotoxicity to a mouse neuroblastoma cell line with LC₅₀ values of 2.7 μM (Nan et al., 2011). A Lyngbya sp. collected in Florida Keys was found to contain epimeric cyclic depsipeptides Porpoisamide A (184) and B (185) (Figure S13) which was weakly cytotoxic to HCT-116 (IC₅₀ = 25, 21 μM respectively) and osteosarcoma U2OS cells (IC₅₀ = 28, 22 μM respectively) (Meickle et al., 2011). Moorea bouillonii (Strawn Is., Palmyra Atoll, Central Pacific Ocean) gave five lipopeptides Lyngbyabellin K (186) and L (187), 7-epi-lyngbyabellin L (188) and Lyngbyabellin M (189) and N (190) (Figure S13). Of note, cyclic metabolites (189) and (190) possess rare monochlorination on the 3-acyloxy-2-methyloctanoate residue, whereas unusual N, N-dimethylvaline containing lyngbyabellin N was strongly cytotoxic to HCT116 colon cancer cell line (IC₅₀ = 40.9 ± 3.3 nM) (Choi et al., 2012). Viequeamides are novel 2, 2-dimethyl-3-hydroxy-7-octynoic acid (Dhoya)-containing cyclic depsipeptides isolated from a shallow subtidal collection of a “button” cyanobacterium Rivularia sp. (Vieques, Puerto Rico). The absolute structures and configurations of major components Viequeamide A-D (191–194) (Figures S13, S14) of the mixture were established by spectroscopic technique. However, viequeamides B–F were not separated out and only viequeamide A showed high cytotoxicity against H460 human lung cancer cells at IC₅₀ 60 ± 10 nM (Boudreau et al., 2012). A new N, N-dimethyl-terminated linear peptide Symplocin A (195) (Figure S14) was produced by Bahamian collection of cyanobacterium Symploca sp. The absolute configuration of symplocin A was done by chiral-phase HPLC of the corresponding 2-naphthacyl esters. Symplocin A showed potent inhibitory effect on protease enzyme cathepsin E with IC₅₀ 300 pM (Molinski et al., 2012). A collection of tropical marine cyanobacterium, M. bouillonii, from New Britain, Papua New Guinea resulted in isolation of a novel cytotoxic cyclic depsipeptide, Bouillonamide (196) (Figure S14). Compound (196) which contains two unique polyketide-derived moieties, a 2-methyl-6-methylamino-hex-5-enoic acid residue and a unit of 3-methyl-5-hydroxy-heptanoic acid have shown mild toxicity against neuron 2a mouse neuroblastoma cells with IC₅₀ 6.0 μM (Tan et al., 2013). A new lipopeptides, Malyngamide 4 (197) (Figure S14) as a moderate inhibitor of several HTCLs have been isolated from marine cyanobacterium Moorea producens collected from the Red Sea, Saudi Arabia (Shaala et al., 2013). The marine cyanobacterium Okeania sp. collected from the coast near Jahana, Okinawa, was the source of Kurahyne B (198) (Figure S14). It showed growth inhibition against HeLa and HL60 cells, with IC₅₀ values of 8.1 and 9.0 μM, respectively (Okamoto et al., 2015).

A culture of marine fungi Fusarium CNL-619 was the source of a new cyclic depsipeptide N-Methylsansalvamide (199) (Figure S15), which showed weak in vitro cytotoxicity against NCI human tumor cell lines (GI₅₀ 8.3 μM) (Cueto et al., 2000). An unidentified fungus isolated from the red alga, Ceradictyon spongiosum (Okinawa) have been shown to produce two linear dodecapeptides, Dictyonamides A (200) and B (201) (Figure S15). Only the compound (200) showed inhibitory effect on cyclin-dependent kinase 4 with IC₅₀ value of 16.5 μg/mL (Komatsu et al., 2001). A culture of marine fungus, Scytalidium sp., collected from Bahamas was the source of two new cyclic heptapeptides Scytalidamides A (202) and B (203) (Figure S15) and both compounds displayed moderate cytotoxicity to the HCT-116 cell line in vitro with IC₅₀ values of 2.7 and 11.0 μM, respectively (Tan et al., 2003a). A strain of Trichoderma virens was isolated from ascidian Didemnum molle and from the surface of a green alga of genus Halimeda from Papua New Guinea, which was the source of two modified dipeptides Trichodermamides A (204) and B (205) (Figure S15). Trichodermamide B has showed significant in vitro cytotoxicity against HCT-116 cells (colon carcinoma) with an IC₅₀ of 0.32 μg/mL (Garo et al., 2003). A fungal strain Exserohilum rostratum associated with a marine cyanobacterial mat produced four moderately cytotoxic cyclic dipeptides Rostratins A–D (206–209) (Figure S15). The structures and absolute configurations of peptides were determined by two-dimensional NMR techniques and Mosher method respectively. Compounds (206–209) exhibit activity against colon carcinoma (HCT-116) with IC₅₀ values of 8.5, 1.9, 0.76, and 16.5 μg/mL, respectively (Tan et al., 2004).

A new cytotoxic cyclodepsipeptide, IB-01212 (210) (Figure S15) was produced by filamentous fungus Clonostachys sp., ESNA-A009 isolated from an unidentified Japanese sponge. IB-01212 was potent cytotoxic to several human tumor cell lines which includes LN-caP (prostrate), SK-BR3 (breast), HT29 (colon), and HELA (cervix) cell lines with GI₅₀ (growth inhibition) in order of 10⁻⁸ M (Cruz et al., 2006). A culture of Zygosporium masonii isolated from a marine cyanobacterium afforded a new cyclic Penta depsipeptide, Zygosporamide (211) (Figure S15), which had significant cytotoxicity in the NCI's 60 cell line panel, CNS cancer cell line SF-268 (GI₅₀ = 6.5 nM) and the renal cancer cell line RXF 393 (GI₅₀ ≤ 5.0 nM) (Oh et al., 2006). Trichoderma reesei isolated from China, Lianyungang collection of sea mud produced moderately cytotoxic Trichoderide A (212) (Figure S15) (Sun et al., 2006). Two new cyclohexadepsipeptides, Spicellamide A (213) and Spicellamide B (214) (Figure S15) obtained from fermentation of Spicellum roseum (Ectyplasia perox, Dominica), exhibited cytotoxicity to neuroblastoma cells with IC₅₀ value of 30 and 6.2 μg/mL respectively (Kralj et al., 2007). Two new cyclic depsipeptides 1962A, cyclo-(d-Leu-Gly-l-Tyr-l-Val-Gly-S-O-Leu) (215), and 1962B, cyclo-(d-Leu-Gly-l-Phe-l-Val-Gly-S-O-Leu) (216) (Figure S15) have been isolated from the fermentation broth of the mangrove endophytic fungus isolated from the leaf of Kandelia candel. Compound (215) only showed activity against human breast cancer MCF-7 cells with an IC₅₀ value of 100 μg/mL (Huang et al., 2007).

Two new cyclic tetrapeptides Microsporins A (217) and B (218) (Figure S15) with potent inhibitors of histone deacetylase (HDAC), cytotoxic to HCT-116 cells (IC₅₀ 0.6 and 8.5 μg/mL) was isolated from the marine-derived fungus Microsporum gypseum (Gu et al., 2007). A Penta decapeptide, Efrapeptin J (219) (Figure S16), a down-regulator of the molecular chaperone GRP78 have been isolated from Tolypocladium sp. (sea mud, Aomori Prefecture, Japan) (Hayakawa et al., 2008). An Australian marine isolate of Aspergillus versicolor (MST-MF495) offered two cyclo pentapeptides, Cotteslosins A (220) and B (221) (Figure S16) (Fremlin et al., 2009). Two new cyclic pentapeptides, Versicotides A (222) and B (223) (Figure S16) came from marine fungus strain ZLN-60, identified as A. versicolor (Zhou et al., 2011). A Cytotoxic lipopeptide Fellutamide F (224) (Figure S16) have been isolated from the sponge-derived fungus A. versicolor with cytotoxicity to several human tumor cells, especially SK-MEL-2 (skin, IC₅₀ 0.67 μM), XF498 (CNS, IC₅₀ 0.14 μM) and HCT15 (Colon, IC₅₀ 0.13 μM) (Lee et al., 2011). Fermentation extract of the marine-derived fungus Acremonium persicinum SCSIO 115 resulted in the discovery of three new cyclo heptapeptides, Cordyheptapeptides C–E (225–227) (Figure S16) with cytotoxicity against SF-268, MCF-7, and NCI-H460 tumor cell line with IC₅₀ values ranging from 2.5 to 12.1 μM (Chen et al., 2012). Chemical investigation of endophytic fungus Bionectria ochroleuca isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan Island (China), lead to discover two new peptides, Pullularins E and F (228-229) (Figure S16). Both compounds exhibited moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC₅₀ values ranging between 0.1 and 6.7 μg/mL (Ebrahim et al., 2012). An unusual anthranilic acid dimer and a d-phenyllactic acid residue containing cyclodepsipeptides Clavatustides A–C (230–232) (Figure S16) were discovered from cultured mycelia and broth of Aspergillus clavatus C2WU isolated from Xenograpsus testudinatus and suppressed proliferation of HTCLs (Jiang et al., 2013; Ye et al., 2014). Aspergillus terreus SCSGAF0162 gave a new cytotoxic (HTCLs) and antiviral (H1N1 and H3N2) cyclic tetrapeptide, Asperterrestide A (233) (Figure S16). Which was cytotoxic toward human carcinoma U937 and MOLT4 cell lines with IC₅₀ values of 6.4 and 6.2 μM, respectively, and also showed inhibitory effects on the influenza virus strains A/WSN/33 (H1N1) and A/Hong Kong/8/68 (H3N2) with IC₅₀ values of 15 and 8.1 μM, respectively (He et al., 2013). A new cyclohexapeptide, Similanamide (234) (Figure S16) was isolated from sponge-associated fungus Aspergillus similanensis KUFA 0013 with weak anticancer activity (Prompanya et al., 2015).

Marine microorganisms have been recognized as one of the most promising groups of organisms from which novel pharmacologically active molecules, with potential benefits against cancer, can be isolated. Recently, several compounds have been emerged as templates for the development of novel anticancer drugs. However the mechanisms implicated in the cytotoxicity of these compounds in tumor cell lines are still largely overlooked but several studies point to an implication in apoptosis. For instance, several compounds were found to inhibit cell growth in a large variety of cancer cell lines, the pathways by which cancer cells are inhibited are still poorly elucidated. In some cases, compounds were found to induce cell death by activation of the apoptotic process; nevertheless the mechanisms underlying the apoptosis still need more investigations. Some compounds were found to create an imbalance in cellular redox potential, with mitochondria representing a central role in the process. However, more studies are needed in order to clarify it. Cell cycle is another disturbed process, mainly due to disruption of the microtubules and actin filaments; however there are only a few studies connecting marine NRPs with alterations in cell cycle and more studies are needed in order to clarify the involvement of these compounds in the process. Even membrane sodium channels can establish interactions with the compounds, revealing its potentially important role in the observed effects. In summary, more investigations are needed in order to clarify the specific targets and the mechanisms that are behind cancer cell cytotoxicity, namely the involvement of the apoptotic process by the implication of functional genomics.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.