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<article article-type="brief-report" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xml:lang="EN">
<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Oral. Health</journal-id>
<journal-title>Frontiers in Oral Health</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Oral. Health</abbrev-journal-title>
<issn pub-type="epub">2673-4842</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/froh.2024.1498117</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Oral Health</subject>
<subj-group>
<subject>Brief Research Report</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Enhancing intraoral radiographic technique: introducing &#x201C;Nallan&#x0027;s Lines&#x201D;</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes"><name><surname>Chaitanya</surname><given-names>Nallan CSK</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="corresp" rid="cor1">&#x002A;</xref><uri xlink:href="https://loop.frontiersin.org/people/2845073/overview"/><role content-type="https://credit.niso.org/contributor-roles/conceptualization/"/><role content-type="https://credit.niso.org/contributor-roles/data-curation/"/><role content-type="https://credit.niso.org/contributor-roles/formal-analysis/"/><role content-type="https://credit.niso.org/contributor-roles/funding-acquisition/"/><role content-type="https://credit.niso.org/contributor-roles/investigation/"/><role content-type="https://credit.niso.org/contributor-roles/methodology/"/><role content-type="https://credit.niso.org/contributor-roles/project-administration/"/><role content-type="https://credit.niso.org/contributor-roles/resources/"/><role content-type="https://credit.niso.org/contributor-roles/software/"/><role content-type="https://credit.niso.org/contributor-roles/supervision/"/><role content-type="https://credit.niso.org/contributor-roles/validation/"/><role content-type="https://credit.niso.org/contributor-roles/visualization/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/></contrib>
<contrib contrib-type="author"><name><surname>Padmanabhan</surname><given-names>Vivek</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref><uri xlink:href="https://loop.frontiersin.org/people/2820854/overview" /><role content-type="https://credit.niso.org/contributor-roles/methodology/"/><role content-type="https://credit.niso.org/contributor-roles/supervision/"/><role content-type="https://credit.niso.org/contributor-roles/validation/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/></contrib>
<contrib contrib-type="author"><name><surname>Islam</surname><given-names>Md Sofiqul</given-names></name>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref><uri xlink:href="https://loop.frontiersin.org/people/2817978/overview" /><role content-type="https://credit.niso.org/contributor-roles/data-curation/"/><role content-type="https://credit.niso.org/contributor-roles/formal-analysis/"/><role content-type="https://credit.niso.org/contributor-roles/investigation/"/><role content-type="https://credit.niso.org/contributor-roles/validation/"/><role content-type="https://credit.niso.org/contributor-roles/visualization/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/></contrib>
<contrib contrib-type="author"><name><surname>Hashim</surname><given-names>Nada Tawfig</given-names></name>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref><uri xlink:href="https://loop.frontiersin.org/people/2692867/overview" /><role content-type="https://credit.niso.org/contributor-roles/resources/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/><role content-type="https://credit.niso.org/contributor-roles/conceptualization/"/><role content-type="https://credit.niso.org/contributor-roles/data-curation/"/><role content-type="https://credit.niso.org/contributor-roles/methodology/"/><role content-type="https://credit.niso.org/contributor-roles/project-administration/"/><role content-type="https://credit.niso.org/contributor-roles/validation/"/></contrib>
<contrib contrib-type="author"><name><surname>Mohammed</surname><given-names>Riham</given-names></name>
<xref ref-type="aff" rid="aff5"><sup>5</sup></xref>
<role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/><role content-type="https://credit.niso.org/contributor-roles/formal-analysis/"/><role content-type="https://credit.niso.org/contributor-roles/validation/"/><role content-type="https://credit.niso.org/contributor-roles/project-administration/"/></contrib>
<contrib contrib-type="author"><name><surname>Allam</surname><given-names>Neeharika Satyajyothi</given-names></name>
<xref ref-type="aff" rid="aff6"><sup>6</sup></xref>
<role content-type="https://credit.niso.org/contributor-roles/methodology/"/><role content-type="https://credit.niso.org/contributor-roles/project-administration/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/><role content-type="https://credit.niso.org/contributor-roles/conceptualization/"/><role content-type="https://credit.niso.org/contributor-roles/visualization/"/></contrib>
<contrib contrib-type="author"><name><surname>Omar</surname><given-names>Jouma Jalal</given-names></name>
<xref ref-type="aff" rid="aff7"><sup>7</sup></xref>
<role content-type="https://credit.niso.org/contributor-roles/conceptualization/"/><role content-type="https://credit.niso.org/contributor-roles/investigation/"/><role content-type="https://credit.niso.org/contributor-roles/methodology/"/><role content-type="https://credit.niso.org/contributor-roles/project-administration/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/><role content-type="https://credit.niso.org/contributor-roles/software/"/></contrib>
<contrib contrib-type="author"><name><surname>Zakaria</surname><given-names>Ahmed</given-names></name>
<xref ref-type="aff" rid="aff7"><sup>7</sup></xref>
<role content-type="https://credit.niso.org/contributor-roles/methodology/"/><role content-type="https://credit.niso.org/contributor-roles/project-administration/"/><role content-type="https://credit.niso.org/contributor-roles/visualization/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/><role content-type="https://credit.niso.org/contributor-roles/formal-analysis/"/><role content-type="https://credit.niso.org/contributor-roles/funding-acquisition/"/><role content-type="https://credit.niso.org/contributor-roles/investigation/"/><role content-type="https://credit.niso.org/contributor-roles/resources/"/></contrib>
<contrib contrib-type="author"><name><surname>Pranathi</surname><given-names>Shaga</given-names></name>
<xref ref-type="aff" rid="aff8"><sup>8</sup></xref><uri xlink:href="https://loop.frontiersin.org/people/2892323/overview" /><role content-type="https://credit.niso.org/contributor-roles/conceptualization/"/><role content-type="https://credit.niso.org/contributor-roles/formal-analysis/"/><role content-type="https://credit.niso.org/contributor-roles/investigation/"/><role content-type="https://credit.niso.org/contributor-roles/methodology/"/><role content-type="https://credit.niso.org/contributor-roles/resources/"/><role content-type="https://credit.niso.org/contributor-roles/supervision/"/><role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/></contrib>
<contrib contrib-type="author"><name><surname>Apoorva</surname><given-names>Mamindla</given-names></name>
<xref ref-type="aff" rid="aff9"><sup>9</sup></xref><role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/><role content-type="https://credit.niso.org/contributor-roles/supervision/"/><role content-type="https://credit.niso.org/contributor-roles/validation/"/><role content-type="https://credit.niso.org/contributor-roles/investigation/"/></contrib>
</contrib-group>
<aff id="aff1"><label><sup>1</sup></label><institution>Department of Oral Radiology and Oral Medicine, RAK College of Dental Sciences, RAK Medical and Health Sciences University</institution>, <addr-line>Ras Al Khaimah</addr-line>, <country>United Arab Emirates</country></aff>
<aff id="aff2"><label><sup>2</sup></label><institution>Department of Pedodontics, RAK College of Dental Sciences, RAK Medical and Health Sciences University</institution>, <addr-line>Ras Al Khaimah</addr-line>, <country>United Arab Emirates</country></aff>
<aff id="aff3"><label><sup>3</sup></label><institution>Department of Restorative Dentistry, RAK College of Dental Sciences, RAK Medical and Health Sciences University</institution>, <addr-line>Ras Al Khaimah</addr-line>, <country>United Arab Emirates</country></aff>
<aff id="aff4"><label><sup>4</sup></label><institution>Department of Periodontics, RAK College of Dental Sciences, RAK Medical and Health Sciences University</institution>, <addr-line>Ras Al Khaimah</addr-line>, <country>United Arab Emirates</country></aff>
<aff id="aff5"><label><sup>5</sup></label><institution>Department of Oral Surgery, RAK College of Dental Sciences, RAK Medical and Health Sciences University</institution>, <addr-line>Ras Al Khaimah</addr-line>, <country>United Arab Emirates</country></aff>
<aff id="aff6"><label><sup>6</sup></label><institution>Department of Dentistry, Tatva Dental Center</institution>, <addr-line>Hyderabad</addr-line>, <country>India</country></aff>
<aff id="aff7"><label><sup>7</sup></label><institution>RAK College of Dental Sciences, RAK Medical and Health Sciences University</institution>, <addr-line>Ras Al Khaimah</addr-line>, <country>United Arab Emirates</country></aff>
<aff id="aff8"><label><sup>8</sup></label><institution>Department of MS healthcare Informatics, Sacred Heart University</institution>, <addr-line>Fairfield, CT</addr-line>, <country>United States</country></aff>
<aff id="aff9"><label><sup>9</sup></label><institution>Department of Oral Medicine and Radiology, Panineeya Dental College</institution>, <addr-line>Hyderabad</addr-line>, <country>India</country></aff>
<author-notes>
<fn fn-type="edited-by"><p><bold>Edited by:</bold> Enrico Nastro Siniscalchi, Kore University of Enna, Italy</p></fn>
<fn fn-type="edited-by"><p><bold>Reviewed by:</bold> Weeraya Tantanapornkul, Naresuan University, Thailand</p>
<p>Oladimeji Adeniyi Akadiri, University of Port Harcourt, Nigeria</p></fn>
<corresp id="cor1"><label>&#x002A;</label><bold>Correspondence:</bold> Nallan CSK Chaitanya <email>nallanchaitanya@gmail.com</email></corresp>
</author-notes>
<pub-date pub-type="epub"><day>20</day><month>12</month><year>2024</year></pub-date>
<pub-date pub-type="collection"><year>2024</year></pub-date>
<volume>5</volume><elocation-id>1498117</elocation-id>
<history>
<date date-type="received"><day>18</day><month>09</month><year>2024</year></date>
<date date-type="accepted"><day>25</day><month>11</month><year>2024</year></date>
</history>
<permissions>
<copyright-statement>&#x00A9; 2024 Chaitanya, Padmanabhan, Islam, Hashim, Mohammed, Allam, Omar, Zakaria, Pranathi and Apoorva.</copyright-statement>
<copyright-year>2024</copyright-year><copyright-holder>Chaitanya, Padmanabhan, Islam, Hashim, Mohammed, Allam, Omar, Zakaria, Pranathi and Apoorva</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License (CC BY)</ext-link>. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p></license>
</permissions>
<abstract><sec><title>Background</title>
<p>Intraoral radiography remains the most widely employed dental radiographic technique for producing geometrically accurate images with minimal distortion and magnification. Despite its common use in the dental office, incorrect projection geometry can be challenging leading to image distortions. To mitigate these inaccuracies specific radiographic landmarks on the face are utilized during periapical radiography. Nallan&#x0027;s lines, proposed in this study may address the perpetual issue.</p>
</sec><sec><title>Material and methods</title>
<p>In this cross over study, the participants were divided into 2 groups, Group A and Group B comprising nine in the each group. The bisecting angle technique was performed by the participants using a gyroscopic device fitted with a LASER with (Study subjects) and without (Controls) utilizing Nallan&#x0027;s lines on a selected group of teeth using a phantom model. Subsequent images were compared with those images obtained by an oral radiologist using the same model for geometric distortions of the images. This comparative analysis aimed to identify potential geometric distortions within the generated images</p>
</sec><sec><title>Results</title>
<p>A paired <italic>t</italic>-test was employed to compare the mean length and breadth of teeth before and after radiographic training. Additionally, a McNemar Test was used to assess the impact of Nallan&#x0027;s lines on technical errors. Both analyses revealed statistically significant improvements post-training (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.01). To determine if radiographic training affected the performance of volunteers and specialists, a Chi-Square Test was conducted to compare error rates. No statistically significant differences were observed between the two groups, both before and after training.</p>
</sec><sec><title>Conclusion</title>
<p>The adoption of Nallan&#x0027;s lines may enhance the accuracy and quality of intraoral radiographic images. By adhering to this geometric framework dental practitioners can minimize geometric distortions and thereby repeated radiographic exposures of patients.</p>
</sec>
</abstract>
<kwd-group>
<kwd>intraoral radiography</kwd>
<kwd>geometric distortion</kwd>
<kwd>periapical projections</kwd>
<kwd>gyroscope</kwd>
<kwd>radiographic reference points</kwd>
</kwd-group><counts>
<fig-count count="3"/>
<table-count count="7"/><equation-count count="0"/><ref-count count="15"/><page-count count="10"/><word-count count="0"/></counts><custom-meta-wrap><custom-meta><meta-name>section-at-acceptance</meta-name><meta-value>Oral and Maxillofacial Surgery</meta-value></custom-meta></custom-meta-wrap>
</article-meta>
</front>
<body><sec id="s1" sec-type="intro"><title>Introduction</title>
<p>Intraoral radiography, utilizing the bisecting technique, is a fundamental diagnostic approach in dentistry with its precession relying significantly on accurate film positioning and the careful angulation of x-ray beam (<xref ref-type="bibr" rid="B1">1</xref>&#x2013;<xref ref-type="bibr" rid="B3">3</xref>). A dental radiologist primary responsibility during this procedure is to ensure optimum film placement through meticulous assessment of both the horizontal and vertical angulation of the x-ray beam (<xref ref-type="bibr" rid="B2">2</xref>). Errors in angulation may lead to image distortions such as elongation or foreshortening of images (<xref ref-type="bibr" rid="B2">2</xref>&#x2013;<xref ref-type="bibr" rid="B4">4</xref>). These technical inaccuracies not only reduce the diagnostic quality of radiographic images but can also result in misdiagnosis and treatment failures, while potentially increasing radiation exposure to patients (<xref ref-type="bibr" rid="B1">1</xref>&#x2013;<xref ref-type="bibr" rid="B4">4</xref>). To address these issues radiographic landmarks are utilized to improve positioning accuracy and minimize the need for repeated exposure (<xref ref-type="bibr" rid="B2">2</xref>).</p>
<p>Standard reference points for intraoral radiography are well-established, with the Ala-tragus line being a crucial guide for directing the x-ray beam in both maxillary and inferior border of mandible for mandibular radiographic exposures. Additionally various anatomical landmarks in the maxilla and mandible serve as standard reference points for x-ray beam alignment particularly in the bisecting technique (<xref ref-type="bibr" rid="B1">1</xref>&#x2013;<xref ref-type="bibr" rid="B4">4</xref>) (<xref ref-type="fig" rid="F1">Figure&#x00A0;1</xref>).</p>
<fig id="F1" position="float"><label>Figure 1</label>
<caption><p>Standard conventional radiographic reference points for maxillary and mandibular teeth periapical projections.</p></caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="froh-05-1498117-g001.tif"/>
</fig>
<p>However the event effectiveness of these landmarks may be limited by anatomical variations and overlapping structures. Studies have shown that using the ala-tragus line as an anatomical reference line in periapical radiographic projections (<xref ref-type="bibr" rid="B2">2</xref>) can significantly improve accuracy in periapical imaging compared to those where it was not utilized as a reference. Nonetheless, visualizing the imaginary ala-tragus line presented certain challenges: Clinicians may occasionally rely on the inferior or superior border of the tragus, experience confusion over the posterior reference points, and required substantial skill and training to orient the x-ray beam accurately to the area of interest. Frankfort&#x0027;s horizontal plane could also be used for maxillary teeth radiographic positions. Furthermore, barring the usage of inferior border of mandible, as a guidance plane, there are no specific radiographic reference lines available for mandibular teeth.</p>
<p>In response to these limitations, a pilot study was conducted to evaluate the effectiveness of modified radiographic reference guidance termed &#x201C;Nallan&#x0027;s Lines.&#x201D; This study aimd to offer a more precise and reliable approach for image acquisition in the bisecting the angle technique. It compared the usage of the standard reference guides and the &#x201C;Nallan Lines&#x201D; for maxillary and mandibular intraoral radiographic projections.</p>
<sec id="s1a"><title>Concept of Nallan&#x0027;s lines</title>
<p>Nallan&#x0027;s lines or Modified radiographic reference lines are confined by specific anatomical boundaries within the mandible and maxilla. In the maxilla, the reference line is an imaginary horizontal line bisecting the triangular region defined by the Frankfort horizontal line, the superior border of the tragus of the ear and the ala of the nose. In the mandible, the line is a horizontal line drawn from there soft tissue of Point B to the gonion on the same side as identify it in this cephalometric analysis. It further bisects the line drawn from corner of mouth to angle of the mandible superiorly and inferior border of the mandible inferiorly (<xref ref-type="fig" rid="F2">Figure&#x00A0;2</xref>).</p>
<fig id="F2" position="float"><label>Figure 2</label>
<caption><p>Shows Nallan&#x0027;s line for maxillary and mandibular radiographic projections. (1) Incisors. (3) Canine. (5) Premolars. (7) Molars.</p></caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="froh-05-1498117-g002.tif"/>
</fig>
</sec>
</sec>
<sec id="s2" sec-type="methods"><title>Materials and methods</title>
<sec id="s2a"><title>Study design</title>
<p>A single-centre, prospective, cross-over study was conducted over a 5 month period a t the Radiology Lab of RAKCODS after obtaining necessary ethical approval. The study was designed as an <italic>in vitro</italic> preclinical investigation which involved 9 participants, including general dental practitioners and dental nurses who routinely make radiographic projections in the dental clinics independently. Undergraduate dental students were trained for dental radiology projections in the radiology lab only from the third year of dental course based on the course curriculum. This necessitated more training before they could make intraoral radiographic projections unassisted. Thus they were excluded from participation in the study. Each participant performed two rounds of radiographic procedures within a 30-day period. This allowed for proper training of the personal and no overlap of both tt old and the new projection techniques. Each participant made at least 10 radiographic projections using the bisecting angle technique on the phantom model with and without a LASER assisted gyroscopic device. The projections included selected maxillary and mandibular teeth within the anterior and posteriors. A phantom head equipped with upper and lower jaws was utilized to simulate the human oral cavity for the radiographic procedures. This provided a consistent and reproducible model for all participants, ensuring standardization across the study. Utilizing the Raosoft sample size calculator, the calculated sample size with a 5&#x0025; margin of error and 95&#x0025; confidence level is 90.</p>
<p>The participants were divided into two groups: Group A (conventional standard projections) and Group B (Nallan Lines). Group A performed intraoral digital radiography using a CCD sensor and Nallan DirectRay device, following the standard radiographic reference points like the ala-tragus line and the inferior border of mandible. Subsequently the same participants assigned as Group B were trained to use Nallan&#x0027;s Lines as radiographic reference and repeated the procedure. The results of both groups were compared to radiographic procedures performed by a specialist oral radiologist using gyroscope-enabled devices with and without usage of the Nallan&#x0027;s lines. The horizontal and vertical angulations for each of the teeth were followed as standard angulations for both the technique without any change.</p>
<p>Nallan-DirectRay is a gyroscope enabled laser device mounted on the position indicating device of the dental radiographic machine. This was innovatively developed device for precise and accurate radiographic reference points on the area of interest. The validity of the device was checked and is under patent evaluation (<xref ref-type="fig" rid="F3">Figure&#x00A0;3</xref>).</p>
<fig id="F3" position="float"><label>Figure 3</label>
<caption><p>Shows Nallan&#x0027;s directRay LASER and gyroscopic device fitted on the x-ray machine for periapical projections.</p></caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="froh-05-1498117-g003.tif"/>
</fig>
<p>The following equipment was employed for the study:
<list list-type="simple">
<list-item><label>&#x2022;</label>
<p><bold>Intraoral x-ray Machine:</bold> Used for generating the radiographic images.</p></list-item>
<list-item><label>&#x2022;</label>
<p><bold>CCD Sensor:</bold> A charge-coupled device sensor was used to capture the x-ray images.</p></list-item>
<list-item><label>&#x2022;</label>
<p><bold>Nallan DirectRay Device:</bold> Utilized to assist in the accurate positioning of the x-ray beam.</p></list-item>
<list-item><label>&#x2022;</label>
<p><bold>Computer:</bold> For storing and analyzing the captured radiographs.</p></list-item>
<list-item><label>&#x2022;</label>
<p><bold>Lead Aprons:</bold> Provided to all participants to ensure radiation protection.</p></list-item>
</list></p>
<p>The landmarks used as guidance for performing radiographic examinations of maxillary and mandibular teeth with standard and Nallan&#x0027;s Lines are provided in <xref ref-type="table" rid="T1">Table&#x00A0;1</xref>.</p>
<table-wrap id="T1" position="float"><label>Table 1</label>
<caption><p>Radiographic landmarks for maxillary and mandibular teeth with Standard and Nallan&#x0027;s lines (<xref ref-type="bibr" rid="B1">1</xref>, <xref ref-type="bibr" rid="B5">5</xref>).</p></caption>
<table frame="hsides" rules="groups">
<colgroup>
<col align="left"/>
<col align="left"/>
<col align="left"/>
</colgroup>
<thead>
<tr>
<th valign="top" align="left"/>
<th valign="top" align="left">Standard reference lines</th>
<th valign="top" align="left">Nallan&#x0027;s lines</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Maxillary incisors:</td>
<td valign="top" align="left">Tip of the nose</td>
<td valign="top" align="left">1&#x2005;cm above the tip of the nose, on the bridge of the nose</td>
</tr>
<tr>
<td valign="top" align="left">Maxillary canine:</td>
<td valign="top" align="left">Ala of nose/Canine eminence</td>
<td valign="top" align="left">On the Nallan&#x0027;s line above the ala tragus line 1&#x2005;cm over the canine eminence</td>
</tr>
<tr>
<td valign="top" align="left">Maxillary premolars:</td>
<td valign="top" align="left">Pupillary perpendicular to A-T line below the pupil of the eye</td>
<td valign="top" align="left">On the Nallan&#x0027;s line, 1&#x2005;cm above the ala tragus line with intersection of a perpendicular line below the pupil of the eye.</td>
</tr>
<tr>
<td valign="top" align="left">Maxillary molars:</td>
<td valign="top" align="left">Outer canthus of the eye perpendicular to ala tragus line./cheek below the outer canthus of the eye and the zygoma at the position of the maxillary second molar</td>
<td valign="top" align="left">On the Nallan&#x0027;s line, 1&#x2005;cm above the ala tragus line with intersection of a perpendicular line from outer canthus of eye.</td>
</tr>
<tr>
<td valign="top" align="left">Mandibular incisors:</td>
<td valign="top" align="left">Tip of the chin below the lower lip and about 1&#x2005;cm lateral to the midline.</td>
<td valign="top" align="left">Soft tissue of point B corresponding to the perpendicular line drawn from philtrum of upper lip to inferior border of mandible</td>
</tr>
<tr>
<td valign="top" align="left">Mandibular canine:</td>
<td valign="top" align="left">Angle of mouth nearly perpendicular to the ala of the nose, over the position of the canine, and about 3&#x2005;cm above the inferior border of the mandible</td>
<td valign="top" align="left">Perpendicular line drawn from corner of mouth to inferior border of mandible and a point taken on the horizontal line drawn from soft tissue of point b to gonion (Nallan&#x0027;s line).</td>
</tr>
<tr>
<td valign="top" align="left">Mandibular premolars:</td>
<td valign="top" align="left">Outer canthus of the eye/below the pupil of the eye and about 3&#x2005;cm above the inferior border of the mandible.</td>
<td valign="top" align="left">An imaginary horizontal line drawn from soft tissue of point b to gonion (Nallan&#x0027;s Lines) and perpendicular line drawn from center of the pupil of the corresponding eye to inferior border of the mandible</td>
</tr>
<tr>
<td valign="top" align="left">Mandibular molars:</td>
<td valign="top" align="left">1&#x2005;cm away from outer canthus of the eye below the outer canthus of the eye about 3&#x2005;cm above the inferior border of the mandible.</td>
<td valign="top" align="left">An imaginary horizontal line drawn from soft tissue of point b to gonion (Nallan&#x0027;s Lines) and a perpendicular line drawn from outer canthus of the corresponding eye to inferior border of the mandible.</td>
</tr>
</tbody>
</table>
</table-wrap>
<p>The radiographs taken by each participant were saved as digital images and were transferred to a computer. The images were organized and analyzed using Microsoft Excel. Parameters such as image quality, clarity of anatomical landmarks, and technical aspects such as the length and breadth of the teeth projected, foreshortening, elongation, apical cut, cone cut and horizontal overlap were systematically recorded, evaluated and compared between the two groups.</p>
</sec>
<sec id="s2b"><title>Statistical analysis</title>
<p>The data were analyzed to evaluate the consistency and accuracy of radiographs taken by both groups. Statistical analysis was performed using SPSS for Windows, Version 16.0 (Chicago, IL). A paired Student&#x0027;s <italic>t</italic>-test was used to compare the mean length and breadth of the tooth (in millimeter) in both the control and study groups before and after training. The McNemar test was employed to compare radiographic errors before and after radiographic training in both groups. level of significance was set at <italic>p</italic>&#x2009;&#x003C;&#x2009;0.05. Bonferroni Correction and Fischer exact test were also performed address issues with small sample size. Intra observer variability obtained for oral radiologist images was 0.8 kappa value.</p>
</sec>
</sec>
<sec id="s3" sec-type="results"><title>Results</title>
<list list-type="simple">
<list-item><label>a.</label>
<p><bold>Images from the Specialist</bold>: A paired <italic>t</italic>-test was used to compare the main length and breadth of teeth (in mm) in radiographs taken by specialist oral radiologist before and radiographic training. No statistically significant differences were found in two length and breadth additionally McNemer test revealed no statistically significant difference in radiographic errors before and after training (<xref ref-type="table" rid="T2">Table&#x00A0;2</xref>).</p></list-item>
<list-item><label>b.</label>
<p><bold>Images from the volunteers</bold>: A paired <italic>t</italic>-test was used to compare the main length and breadth of teeth before and after radiographic training in volunteers. A statistically significant difference (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.01) was observed in tooth length. The McNemer test revealed significant reduction in elongation, apical cut, and horizontal overlap errors after training. While improvements were noted in foreshortening and cone cut errors, these differences were not statistically significant (<xref ref-type="table" rid="T2">Table&#x00A0;2</xref>). These findings suggest that training in the use of Nallan&#x0027;s line as a new radiographic reference significantly improved radiographic image accuracy (<xref ref-type="table" rid="T3">Table&#x00A0;3</xref>).</p></list-item>
<list-item><label>c.</label>
<p><bold>Comparison of both specialist and volunteer radiographic images</bold>: A chi-square test was used to compare radiographic errors between volunteers and the specialist radiologist before and after training. No statistically significant differences were found between the two groups for any type of radiographic error both pre- and post-training. However despite the lack of statistical significance, observable improvements were noted in the volunteers&#x0027; performance after training, particularly in reducing the frequency of foreshortening and apex cut errors. These improvements suggest that radiographic training had a positive impact on reducing certain types of radiographic errors among study subjects, bringing their performance closer to the standard set by the specialist radiologist (<xref ref-type="table" rid="T4">Tables&#x00A0;4A</xref>, <xref ref-type="table" rid="T5">4B</xref>).</p></list-item>
<list-item><label>d.</label>
<p><bold>Bonferroni Correction</bold>: Initially, the <italic>p</italic>-values were calculated for each type of radiographic error (e.g., foreshortening, elongation, apex cut, cone cut, and horizontal overlap) to compare the study subjects with the gold standard (radiologist). All parameters (Foreshortening, Elongation, Apex Cut, Cone Cut, and Horizontal Overlap) showed non-significant differences in radiographic error rates between study subjects and the radiologist, both before and after using Nallan&#x0027;s lines. Adjusting the <italic>p</italic>-values (e.g., with the Bonferroni correction) was important because multiple chi-square tests were performed to compare radiographic error rates between study subjects and the gold standard, both before and after intervention. The adjustment ensured that any &#x201C;significant&#x201D; findings were genuinely meaningful and not the result of chance due to the multiple comparisons made. After applying the Bonferroni correction to account for multiple comparisons, the adjusted <italic>p</italic>-values increased, and all were effectively rounded up to 1.0. This strict correction method minimized the chance of obtaining a false positive result (Type I error) across the multiple tests conducted. With the Bonferroni correction, no differences reached statistical significance during comparison. There was requirement of extra evidence to conclude that Nallan&#x0027;s lines, by increasing the sample size, made a significant impact on reducing radiographic errors when comparing study subjects to the radiologist&#x0027;s standards. Please refer to <xref ref-type="table" rid="T3">Table&#x00A0;3</xref> for detailed <italic>p</italic>-values and adjusted <italic>p</italic>-values (<xref ref-type="table" rid="T6">Table&#x00A0;5</xref>).</p></list-item>
<list-item><label>e.</label>
<p><bold>Fisher&#x0027;s Exact Test</bold>: To verify the findings and address potential issues with small sample sizes, Fisher&#x0027;s Exact Test was conducted for each parameter, as this test was more suitable for contingency tables with low frequencies in certain categories. Specifically, the Fisher <italic>p</italic>-values were as follows: for foreshortening, 0.47 (before) and 0.36 (after); for elongation, 0.46 (before) and 1.00 (after); for apex cut, 0.35 (before) and 1.00 (after); for cone cut, 0.55 (before) and 1.00 (after); and for horizontal overlap, 0.94 (before) and 1.00 (after). (<xref ref-type="table" rid="T7">Table&#x00A0;6</xref>)</p></list-item>
</list>
<table-wrap id="T2" position="float"><label>Table 2</label>
<caption><p>Shows comparison of mean length &#x0026; breadth of the tooth (in mm) and radiographic errors by specialist radiologist with and without Nallan&#x0027;s lines.</p></caption>
<table frame="hsides" rules="groups">
<colgroup>
<col align="left"/>
<col align="left"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
</colgroup>
<thead>
<tr>
<th valign="top" align="left" colspan="7">Comparison of mean Length &#x0026; Breadth of the tooth (in mm) before and after using Nallan&#x0027;s lines by Student Paired <italic>t</italic> test</th>
</tr>
<tr>
<th valign="top" align="left">Parameters</th>
<th valign="top" align="center">Time</th>
<th valign="top" align="center"><italic>N</italic></th>
<th valign="top" align="center">Mean</th>
<th valign="top" align="center">SD</th>
<th valign="top" align="center">Mean Diff</th>
<th valign="top" align="center"><italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Length</td>
<td valign="top" align="left">Before</td>
<td valign="top" align="center">10</td>
<td valign="top" align="center">23.35</td>
<td valign="top" align="center">3.17</td>
<td valign="top" align="center" rowspan="2">&#x2212;0.39</td>
<td valign="top" align="center" rowspan="2">0.70</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">After</td>
<td valign="top" align="center">10</td>
<td valign="top" align="center">23.74</td>
<td valign="top" align="center">4.93</td>
</tr>
<tr>
<td valign="top" align="left">Breadth</td>
<td valign="top" align="left">Before</td>
<td valign="top" align="center">10</td>
<td valign="top" align="center">8.29</td>
<td valign="top" align="center">1.81</td>
<td valign="top" align="center" rowspan="2">0.07</td>
<td valign="top" align="center" rowspan="2">0.70</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">After</td>
<td valign="top" align="center">10</td>
<td valign="top" align="center">8.22</td>
<td valign="top" align="center">1.74</td>
</tr>
<tr>
<th valign="top" align="left" colspan="7">Comparison of radiographic errors before and after using Nallan&#x0027;s lines by McNemar Test</th>
</tr>
<tr>
<th valign="top" align="left" rowspan="2" colspan="1">Parameter</th>
<th valign="top" align="left" rowspan="2" colspan="1">Category</th>
<th valign="top" align="center" colspan="2">Before</th>
<th valign="top" align="center" colspan="2">After</th>
<th valign="top" align="center" rowspan="2" colspan="1"><italic>p</italic>-value</th>
</tr>
<tr>
<th valign="top" align="center" colspan="1"><italic>n</italic></th>
<th valign="top" align="center" colspan="1">&#x0025;</th>
<th valign="top" align="center" colspan="1"><italic>n</italic></th>
<th valign="top" align="center" colspan="1">&#x0025;</th>
</tr>
<tr>
<td valign="top" align="left">Foreshortening</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">6</td>
<td valign="top" align="center">60&#x0025;</td>
<td valign="top" align="center">10</td>
<td valign="top" align="center">100&#x0025;</td>
<td valign="top" align="center" rowspan="2">0.13</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">4</td>
<td valign="top" align="center">40&#x0025;</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Elongation</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">6</td>
<td valign="top" align="center">60&#x0025;</td>
<td valign="top" align="center">10</td>
<td valign="top" align="center">100&#x0025;</td>
<td valign="top" align="center" rowspan="2">0.13</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">4</td>
<td valign="top" align="center">40&#x0025;</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Cone Cut</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">10</td>
<td valign="top" align="center">100&#x0025;</td>
<td valign="top" align="center">9</td>
<td valign="top" align="center">90&#x0025;</td>
<td valign="top" align="center" rowspan="2">1.00</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0&#x0025;</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">10&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Apical Cut</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">7</td>
<td valign="top" align="center">70&#x0025;</td>
<td valign="top" align="center">9</td>
<td valign="top" align="center">90&#x0025;</td>
<td valign="top" align="center" rowspan="2">0.63</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">3</td>
<td valign="top" align="center">30&#x0025;</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">10&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Horizontal Overlap</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">7</td>
<td valign="top" align="center">70&#x0025;</td>
<td valign="top" align="center">8</td>
<td valign="top" align="center">80&#x0025;</td>
<td valign="top" align="center" rowspan="2">1.00</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">3</td>
<td valign="top" align="center">30&#x0025;</td>
<td valign="top" align="center">2</td>
<td valign="top" align="center">20&#x0025;</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="T3" position="float"><label>Table 3</label>
<caption><p>Shows comparison of mean length &#x0026; breadth of the tooth (in mm) and radiographic errors by participant groups with and without Nallan&#x0027;s lines.</p></caption>
<table frame="hsides" rules="groups">
<colgroup>
<col align="left"/>
<col align="left"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
</colgroup>
<thead>
<tr>
<th valign="top" align="left" colspan="7">Comparison of mean Length &#x0026; Breadth of the tooth (in mm) [study subjects] before and after using Nallan&#x0027;s lines by Student Paired <italic>t</italic> test</th>
</tr>
<tr>
<th valign="top" align="left">Parameters</th>
<th valign="top" align="center">Time</th>
<th valign="top" align="center"><italic>N</italic></th>
<th valign="top" align="center">Mean</th>
<th valign="top" align="center">SD</th>
<th valign="top" align="center">Mean Diff</th>
<th valign="top" align="center"><italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Length</td>
<td valign="top" align="left">Before</td>
<td valign="top" align="center">81</td>
<td valign="top" align="center">22.05</td>
<td valign="top" align="center">4.15</td>
<td valign="top" align="center" rowspan="2">1.29</td>
<td valign="top" align="center" rowspan="2">0.01&#x002A;</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">After</td>
<td valign="top" align="center">81</td>
<td valign="top" align="center">20.76</td>
<td valign="top" align="center">3.75</td>
</tr>
<tr>
<td valign="top" align="left">Breadth</td>
<td valign="top" align="left">Before</td>
<td valign="top" align="center">80</td>
<td valign="top" align="center">7.77</td>
<td valign="top" align="center">2.12</td>
<td valign="top" align="center" rowspan="2">0.27</td>
<td valign="top" align="center" rowspan="2">0.28</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">After</td>
<td valign="top" align="center">80</td>
<td valign="top" align="center">7.50</td>
<td valign="top" align="center">2.19</td>
</tr>
<tr>
<th valign="top" align="left" colspan="7">Comparison of radiographic errors [study subjects] before and after using Nallan&#x0027;s lines by McNemar Test</th>
</tr>
<tr>
<th valign="top" align="left" rowspan="2" colspan="1">Parameter</th>
<th valign="top" align="left" rowspan="2" colspan="1">Category</th>
<th valign="top" align="center" colspan="2">Before</th>
<th valign="top" align="center" colspan="2">After</th>
<th valign="top" align="center" rowspan="2" colspan="1"><italic>p</italic>-value</th>
</tr>
<tr>
<th valign="top" align="center" colspan="1"><italic>n</italic></th>
<th valign="top" align="center" colspan="1">&#x0025;</th>
<th valign="top" align="center" colspan="1"><italic>n</italic></th>
<th valign="top" align="center" colspan="1">&#x0025;</th>
</tr>
<tr>
<td valign="top" align="left">Foreshortening</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">65</td>
<td valign="top" align="center">72.2&#x0025;</td>
<td valign="top" align="center">72</td>
<td valign="top" align="center">81.8&#x0025;</td>
<td valign="top" align="center" rowspan="2">0.22</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">25</td>
<td valign="top" align="center">27.8&#x0025;</td>
<td valign="top" align="center">16</td>
<td valign="top" align="center">18.2&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Elongation</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">66</td>
<td valign="top" align="center">73.3&#x0025;</td>
<td valign="top" align="center">81</td>
<td valign="top" align="center">91.0&#x0025;</td>
<td valign="top" align="center" rowspan="2">0.003&#x002A;</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">24</td>
<td valign="top" align="center">26.7&#x0025;</td>
<td valign="top" align="center">8</td>
<td valign="top" align="center">9.0&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Cone Cut</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">76</td>
<td valign="top" align="center">84.4&#x0025;</td>
<td valign="top" align="center">72</td>
<td valign="top" align="center">80.0&#x0025;</td>
<td valign="top" align="center" rowspan="2">0.50</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">14</td>
<td valign="top" align="center">15.6&#x0025;</td>
<td valign="top" align="center">18</td>
<td valign="top" align="center">20.0&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Apical Cut</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">49</td>
<td valign="top" align="center">54.4&#x0025;</td>
<td valign="top" align="center">82</td>
<td valign="top" align="center">91.1&#x0025;</td>
<td valign="top" align="center" rowspan="2">&#x003C;0.001&#x002A;</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">41</td>
<td valign="top" align="center">45.6&#x0025;</td>
<td valign="top" align="center">8</td>
<td valign="top" align="center">8.9&#x0025;</td>
</tr>
<tr>
<td valign="top" align="left">Horizontal Overlap</td>
<td valign="top" align="left">Absent</td>
<td valign="top" align="center">57</td>
<td valign="top" align="center">63.3&#x0025;</td>
<td valign="top" align="center">75</td>
<td valign="top" align="center">83.3&#x0025;</td>
<td valign="top" align="center" rowspan="2">0.001&#x002A;</td>
</tr>
<tr>
<td valign="top" align="left"/>
<td valign="top" align="left">Present</td>
<td valign="top" align="center">33</td>
<td valign="top" align="center">36.7&#x0025;</td>
<td valign="top" align="center">15</td>
<td valign="top" align="center">16.7&#x0025;</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="T4" position="float"><label>Table 4A</label>
<caption><p>Comparison of mean length &#x0026; breadth of the tooth (in mm) b/w study subjects and radiologist before and after using Nallan&#x0027;s lines by independent student <italic>t</italic> test.</p></caption>
<table frame="hsides" rules="groups">
<colgroup>
<col align="left"/>
<col align="left"/>
<col align="left"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
</colgroup>
<thead>
<tr>
<th valign="top" align="left">Parameter</th>
<th valign="top" align="center">Time</th>
<th valign="top" align="center">Time</th>
<th valign="top" align="center">Mean</th>
<th valign="top" align="center">SD</th>
<th valign="top" align="center">Mean Diff</th>
<th valign="top" align="center"><italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left" rowspan="4">Length</td>
<td valign="top" align="left" rowspan="2">Before</td>
<td valign="top" align="left">Study Subjects</td>
<td valign="top" align="center">22.08</td>
<td valign="top" align="center">4.14</td>
<td valign="top" align="center" rowspan="2">&#x2212;1.27</td>
<td valign="top" align="center" rowspan="2">0.35</td>
</tr>
<tr>
<td valign="top" align="left">Gold Std.</td>
<td valign="top" align="center">23.35</td>
<td valign="top" align="center">3.17</td>
</tr>
<tr>
<td valign="top" align="left" rowspan="2">After</td>
<td valign="top" align="left">Study Subjects</td>
<td valign="top" align="center">20.67</td>
<td valign="top" align="center">3.78</td>
<td valign="top" align="center" rowspan="2">&#x2212;3.08</td>
<td valign="top" align="center" rowspan="2">0.02&#x002A;</td>
</tr>
<tr>
<td valign="top" align="left">Gold Std.</td>
<td valign="top" align="center">23.74</td>
<td valign="top" align="center">4.93</td>
</tr>
<tr>
<td valign="top" align="left" rowspan="4">Breadth</td>
<td valign="top" align="left" rowspan="2">Before</td>
<td valign="top" align="left">Study Subjects</td>
<td valign="top" align="center">7.73</td>
<td valign="top" align="center">2.18</td>
<td valign="top" align="center" rowspan="2">&#x2212;0.56</td>
<td valign="top" align="center" rowspan="2">0.44</td>
</tr>
<tr>
<td valign="top" align="left">Gold Std.</td>
<td valign="top" align="center">8.29</td>
<td valign="top" align="center">1.81</td>
</tr>
<tr>
<td valign="top" align="left" rowspan="2">After</td>
<td valign="top" align="left">Study Subjects</td>
<td valign="top" align="center">7.55</td>
<td valign="top" align="center">2.24</td>
<td valign="top" align="center" rowspan="2">&#x2212;0.67</td>
<td valign="top" align="center" rowspan="2">0.36</td>
</tr>
<tr>
<td valign="top" align="left">Gold Std.</td>
<td valign="top" align="center">8.22</td>
<td valign="top" align="center">1.74</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="T5" position="float"><label>Table 4B</label>
<caption><p>Comparison of radiographic errors between study subjects and radiologist before and after using Nallan&#x0027;s lines by Chi square test.</p></caption>
<table frame="hsides" rules="groups">
<colgroup>
<col align="left"/>
<col align="left"/>
<col align="center"/>
<col align="center"/>
<col align="center"/>
</colgroup>
<thead>
<tr>
<th valign="top" align="left">Parameter</th>
<th valign="top" align="center">Condition</th>
<th valign="top" align="center">Study subjects (&#x0025;)</th>
<th valign="top" align="center">Gold Standard (&#x0025;)</th>
<th valign="top" align="center"><italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left" rowspan="2">Foreshortening</td>
<td valign="top" align="left">Before Nallan&#x0027;s Lines</td>
<td valign="top" align="center">27.8</td>
<td valign="top" align="center">40.0</td>
<td valign="top" align="center">0.42</td>
</tr>
<tr>
<td valign="top" align="left">After Nallan&#x0027;s Lines</td>
<td valign="top" align="center">18.2</td>
<td valign="top" align="center">0.0</td>
<td valign="top" align="center">0.14</td>
</tr>
<tr>
<td valign="top" align="left" rowspan="2">Elongation</td>
<td valign="top" align="left">Before Nallan&#x0027;s Lines</td>
<td valign="top" align="center">26.7</td>
<td valign="top" align="center">40.0</td>
<td valign="top" align="center">0.37</td>
</tr>
<tr>
<td valign="top" align="left">After Nallan&#x0027;s Lines</td>
<td valign="top" align="center">9.0</td>
<td valign="top" align="center">0.0</td>
<td valign="top" align="center">0.32</td>
</tr>
<tr>
<td valign="top" align="left" rowspan="2">Apex cut</td>
<td valign="top" align="left">Before Nallan&#x0027;s Lines</td>
<td valign="top" align="center">15.6</td>
<td valign="top" align="center">0.0</td>
<td valign="top" align="center">0.18</td>
</tr>
<tr>
<td valign="top" align="left">After Nallan&#x0027;s Lines</td>
<td valign="top" align="center">20.0</td>
<td valign="top" align="center">10.0</td>
<td valign="top" align="center">0.44</td>
</tr>
<tr>
<td valign="top" align="left" rowspan="2">Cone cut</td>
<td valign="top" align="left">Before Nallan&#x0027;s Lines</td>
<td valign="top" align="center">45.6</td>
<td valign="top" align="center">30.0</td>
<td valign="top" align="center">0.35</td>
</tr>
<tr>
<td valign="top" align="left">After Nallan&#x0027;s Lines</td>
<td valign="top" align="center">8.9</td>
<td valign="top" align="center">10.0</td>
<td valign="top" align="center">0.91</td>
</tr>
<tr>
<td valign="top" align="left" rowspan="2">Horizontal overlap</td>
<td valign="top" align="left">Before Nallan&#x0027;s Lines</td>
<td valign="top" align="center">36.7</td>
<td valign="top" align="center">30.0</td>
<td valign="top" align="center">0.68</td>
</tr>
<tr>
<td valign="top" align="left">After Nallan&#x0027;s Lines</td>
<td valign="top" align="center">16.7&#x0025;</td>
<td valign="top" align="center">20.0&#x0025;</td>
<td valign="top" align="center">0.79</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="T6" position="float"><label>Table 5</label>
<caption><p>Chi-Square test results with adjusted <italic>p</italic>-values (Bonferroni correction).</p></caption>
<table frame="hsides" rules="groups">
<colgroup>
<col align="left"/>
<col align="center"/>
<col align="center"/>
</colgroup>
<thead>
<tr>
<th valign="top" align="left">Comparison</th>
<th valign="top" align="center">Chi-Square <italic>p</italic>-value</th>
<th valign="top" align="center">Adjusted Chi-Square <italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Foreshortening before</td>
<td valign="top" align="center">0.42</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Foreshortening after</td>
<td valign="top" align="center">0.14</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Elongation before</td>
<td valign="top" align="center">0.37</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Elongation after</td>
<td valign="top" align="center">0.32</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Apex cut before</td>
<td valign="top" align="center">0.18</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Apex cut after</td>
<td valign="top" align="center">0.44</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Cone cut before</td>
<td valign="top" align="center">0.35</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Cone cut after</td>
<td valign="top" align="center">0.91</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Horizontal overlap before</td>
<td valign="top" align="center">0.68</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Horizontal overlap after</td>
<td valign="top" align="center">0.79</td>
<td valign="top" align="center">1.0</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="T7" position="float"><label>Table 6</label>
<caption><p>Fisher&#x0027;s exact test results with adjusted <italic>p</italic>-values (Bonferroni correction).</p></caption>
<table frame="hsides" rules="groups">
<colgroup>
<col align="left"/>
<col align="center"/>
<col align="center"/>
</colgroup>
<thead>
<tr>
<th valign="top" align="left">Comparison</th>
<th valign="top" align="center">Fisher <italic>p</italic>-value</th>
<th valign="top" align="center">Adjusted Fisher <italic>p</italic>-value</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Foreshortening before</td>
<td valign="top" align="center">0.47</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Foreshortening after</td>
<td valign="top" align="center">0.36</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Elongation before</td>
<td valign="top" align="center">0.46</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Elongation after</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Apex cut before</td>
<td valign="top" align="center">0.35</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Apex cut After</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Cone cut before</td>
<td valign="top" align="center">0.55</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Cone cut after</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Horizontal overlap before</td>
<td valign="top" align="center">0.94</td>
<td valign="top" align="center">1.0</td>
</tr>
<tr>
<td valign="top" align="left">Horizontal overlap after</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.0</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s4" sec-type="discussion"><title>Discussion</title>
<p>This pilot study aimed to evaluate effectiveness of modified radiographic reference lines, termed &#x201C;Nallan&#x0027;s lines&#x201D;, compared to conventional standard lines (ala-tragus lines, Frankfurt horizontal plane) in intraoral periapical radiography. The study findings indicated that the implementation of Nallan&#x0027;s lines significantly improved the accuracy of radiographic images, particularly by reducing elongation of images, apical cut and horizontal overlap errors. This study aligned with Al-Safi who suggested developing a new method in the bisecting angle technique to enhance radiographic outcomes rather than relying on the traditional approaches. However, any specific new method or approach for radiographic projections were not discussed (<xref ref-type="bibr" rid="B2">2</xref>). The Nallan&#x0027;s lines could represent an evolution in this approach, offering a practical solution for dental practitioners aiming to improve radiographic precision and reduce errors.</p>
<p>The findings demonstrated a statistically significant improvement in the mean length measurements of teeth following radiographic training with Nallan&#x0027;s lines (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.01). This indicates that the modified reference lines enhance image positioning accuracy, contributing to more precise diagnosis and treatment planning based on radiographic images. The absence of significant changes in the control group emphasizes the limitations of traditional reference points which might be more susceptible to variations in anatomy and overlapping structures.</p>
<p>Our results also showed substantial reductions in specific radiographic errors in the study group post-training. Notably, elongation errors decreased from 26.7&#x0025; to 9.0&#x0025; (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.003), and apical cut errors dropped from 45.6&#x0025; to 8.9&#x0025; (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001). Horizontal overlap errors also significantly reduced (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001). These improvements underscore the potential of Nallan&#x0027;s Lines to minimize common technical errors, thereby enhancing image quality and reducing the need for repeated exposures, which ultimately supports better patient safety by lowering cumulative radiation doses.</p>
<p>A systematic review highlighted that the reject rates and error sources in dento-maxillofacial radiography, focusing on intraoral, extra-oral, and CBCT imaging. It also projected that rejection rates were highest for intraoral images, especially periapical radiographs (16.38&#x0025;), primarily due to positioning errors and patient discomfort. This resonated with our work on Nallan&#x0027;s Lines, as improved positioning methods could reduce these issue significantly (<xref ref-type="bibr" rid="B6">6</xref>). In the case of extraoral radiography, the reject rate averaged nearly about 4&#x0025; for panoramic images and 6&#x0025; for lateral cephalograms, with patient movement as a main error source. The study also noted a 2.77&#x0025; rejection rate for CBCT images, where patient stability and head position significantly impacted image quality, suggesting that clearer anatomical references and stability tools, similar to the goals of Nallan&#x0027;s Lines, could reduce errors (<xref ref-type="bibr" rid="B6">6</xref>). The review also underscored the need for accurate reference points, adequate operator training, and ergonomic adjustments in positioning, which alignd with our findings on Nallan&#x0027;s Lines&#x0027; effectiveness in enhancing radiographic accuracy and reducing error rates, such as elongation and apical cut (<xref ref-type="bibr" rid="B6">6</xref>).</p>
<p>A study investigated the proficiency of general dental practitioners in Saudi Arabia with intraoral radiographic techniques. It highlighted variations in technique availability and ease of use, suggesting that standardized training could enhance accuracy in radiographic projections and image. It underscored the importance of precise training in radiographic positioning for improved diagnostic outcomes and reduced errors (<xref ref-type="bibr" rid="B7">7</xref>).</p>
<p>A more recent review demonstrated that though paralleling technique of dental periapical radiography was considered a better approach for endodontic and periodontal treatment plans, the technique might be a disadvantageous for Asian population group because of space insufficiency in maxillary region. Hence a more practical approach with different radiographic reference points were used in this study which may be employed for any population group (<xref ref-type="bibr" rid="B8">8</xref>).</p>
<p>The present study was in agreement with the another study describing the periapical radiographic errors related to apical cut and image contrast (<xref ref-type="bibr" rid="B9">9</xref>, <xref ref-type="bibr" rid="B10">10</xref>). Some studies also demonstrated the need for check list or usage of rectangular collimation for dental radiographic projections in order to reduce the errors during the procedures and images obtained. It may be noted that though the check list might not had positive significant correlation between check list use and error occurrence, these new recommendations and modifications may improve the quality of images obtained thereby reducing patient radiation exposure (<xref ref-type="bibr" rid="B11">11</xref>, <xref ref-type="bibr" rid="B12">12</xref>). These findings are consistent with another similar study emphasizing the importance of accurate reference points in the intraoral radiography.</p>
<p>The present study used an instrument called Nallan&#x0027;s DirectRay. It is a gyroscope enabled laser device mounted on the position indicating device of the dental radiographic machine. The device helped in precise placement of the position indicating device of the x-ray machine on the phantom model with a digital display of vertical angulations. The validity of the device was checked and under patent evaluation.</p>
<p>Shetty et al. (<xref ref-type="bibr" rid="B4">4</xref>) discussed the future potential of laser-guided intraoral radiography for improving accuracy in radiographic procedures. Similarly, Chau et al. (<xref ref-type="bibr" rid="B13">13</xref>) and Zamani et al. (<xref ref-type="bibr" rid="B14">14</xref>) demonstrated that using laser-guided indicators significantly reduced technical errors in radiography (<xref ref-type="bibr" rid="B15">15</xref>). Our study supported these findings by demonstrating that Nallan&#x0027;s lines, used in conjunction with gyroscopic laser guided approach, could serve purpose of enhancing accuracy.</p>
<p>When comparing the study subjects to the specialist radiologist, the study group exhibited a closer approximation of the gold standard in terms of radiographic error rates, post-training. Although not all improvements where statistically significant, the trend suggested a positive impact of the modified reference lines on the radiographic accuracy. Despite the lack of statistically significant differences, several aspects suggested that Nallan&#x0027;s lines offer practical value that may not be fully captured by statistical results alone. Clinically, even modest reductions in radiographic errors&#x2014;such as foreshortening, elongation, and apex cut could enhance diagnostic clarity and patient outcomes. After implementing Nallan&#x0027;s lines, we observed directional improvements across parameters, with increased rates of error-free images in study subjects, approaching the accuracy achieved by the radiologist. In the clinical context, these incremental improvements could be meaningful, particularly for less-experienced practitioners who may benefit from additional guidance in positioning techniques.</p>
<p>Furthermore, Nallan&#x0027;s lines show significant educational potential as a training aid. Visual references, like those provided by this device, could help students and early-career clinicians develop better spatial awareness and technical accuracy, reinforcing proper positioning habits. This is especially valuable in training environments, where proficiency in radiographic positioning can be challenging to master quickly. While these trends may not appear statistically significant in the current study, repeated use and familiarity with Nallan&#x0027;s lines could, over time, lead to more consistent and accurate positioning, ultimately reducing the likelihood of errors in clinical practice.</p>
<sec id="s4a"><title>Limitations and recommendations</title>
<p>Future research may yield stronger evidence of the device&#x0027;s efficacy, especially with larger sample sizes or longer study durations. A larger cohort could provide the statistical power needed to detect smaller, yet clinically meaningful, effects that a limited sample size might obscure. Similarly, a randomized control trial assessing the device&#x0027;s impact over time could provide a more comprehensive understanding of its benefits, particularly if it focuses on practical improvements in radiographic accuracy within routine clinical settings. Another potential benefit lies in reducing the need for repeat exposures, which enhances patient safety by limiting unnecessary radiation exposure. This aligns with a patient-centric approach to healthcare, where even minor reductions in radiographic errors have broader implications for patient safety and care quality.</p>
</sec>
</sec>
<sec id="s5" sec-type="conclusions"><title>Conclusion</title>
<p>The introduction of Nallan&#x0027;s lines as modified radiographic reference points demonstrated a substantial improvement in the accuracy of intraoral periapical radiographs, with particular effectiveness in reducing elongation, apical cut, and horizontal overlap errors. This advancement suggested that Nallan&#x0027;s lines may offer a robust framework for improving radiographic precision, leading to more accurate diagnosis and enhanced treatment planning.</p>
</sec>
</body>
<back>
<sec id="s6" sec-type="data-availability"><title>Data availability statement</title>
<p>The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.</p>
</sec>
<sec id="s7" sec-type="author-contributions"><title>Author contributions</title>
<p>NC: Conceptualization, Data curation, Formal Analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. VP: Methodology, Supervision, Validation, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. MI: Data curation, Formal Analysis, Investigation, Validation, Visualization, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. NH: Resources, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing, Conceptualization, Data curation, Methodology, Project administration, Validation. RM: Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing, Formal analysis, Validation, Project administration. NA: Methodology, Project administration, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing, Conceptualization, Visualization. JO: Conceptualization, Investigation, Methodology, Project administration, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing, Software. AZ: Methodology, Project administration, Visualization, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing, Formal Analysis, Funding acquisition, Investigation, Resources. SP: Conceptualization, Formal Analysis, Investigation, Methodology, Resources, Supervision, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. MA: Writing &#x2013; review &#x0026; editing, Supervision, Validation, Investigation.</p>
</sec>
<sec id="s8" sec-type="funding-information"><title>Funding</title>
<p>The author(s) declare that no financial support was received for the research, authorship, and/or publication of this article.</p>
</sec>
<sec id="s9" sec-type="COI-statement"><title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec id="s10" sec-type="disclaimer"><title>Publisher&#x0027;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
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