AUTHOR=Du Qingwu , Liu Wenting , Mei Ting , Wang Jingya , Qin Tingting , Huang Dingzhi 

TITLE=Prognostic and immunological characteristics of CDK1 in lung adenocarcinoma: A systematic analysis

JOURNAL=Frontiers in Oncology

VOLUME=Volume 13 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1128443

DOI=10.3389/fonc.2023.1128443

ISSN=2234-943X

ABSTRACT=Background: Cyclin-dependent kinases (CDKs) play a key role in cell proliferation in lung adenocarcinoma (LUAD). Comprehensive analysis of CDKs to elucidate their clinical significance and interactions with the tumor immune microenvironment is needed.
Methods: RNA expression, somatic mutation, copy number variation, and single-cell RNA sequencing data were downloaded from public datasets. First, we comprehensively evaluated the expression profile and prognostic characteristics of 26 CDKs in LUAD, and CDK1 was selected as a candidate for further analysis. Then, a systematic analysis was performed to explore the relationships of CDK1 with clinical characteristics and tumor immune microenvironment factors in LUAD.
Results: Expression of CDK1 were markedly upregulated both on mRNA and protein level in LUAD. What’s more, overexpression of CDK1 was related to poor clinical outcomes. CDK1 coexpressed genes were mainly involved in the process of cell cycle, DNA repair, and also in the p53 signaling pathway. In addition, CDK1 expression was found to be correlated with the expression of multiple immunomodulators and chemokines, both of them participate in activating and suppressing the immune microenvironment, with increased infiltration of numerous immune cells, including CD4+ T cells and M1 macrophages. Patients with high CDK1 expression tended to have a poor response to immunotherapy but were sensitive to multiple chemotherapy and targeted drugs. The MDK-NCL and SPP1-CD44 ligand‒receptor pairs were markedly activated in the intercellular communication network. CDK1 was an independent prognostic factor for LUAD and improved the predictive ability of TNM stage for overall survival.
Conclusion: CDK1 plays an essential role in reshaping the tumor immune microenvironment and might be a prognostic and treatment biomarker in LUAD.
Keywords: CDK1; lung adenocarcinoma; tumor immune microenvironment; biomarker; prognosis