AUTHOR=He Dong , Qin Zhen , Liu Zihao , Ji Xiaoshuai , Gao Jiajia , Guo Hua , Yang Fan , Fan Haitao , Wei Yanbang , Wang Zixiao , Liu Qian , Pang Qi 

TITLE=Comprehensive Analysis of the Prognostic Value and Immune Infiltration of Butyrophilin Subfamily 2/3 (BTN2/3) Members in Pan-Glioma

JOURNAL=Frontiers in Oncology

VOLUME=Volume 12 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2022.816760

DOI=10.3389/fonc.2022.816760

ISSN=2234-943X

ABSTRACT=Abstract
The BTN2/3 subfamilies are overexpressed in many cancers, including panglioma (low- and high-grade glioma). However, the expression and prognosis of BTN2/3 subfamilies and tumor-infiltrating lymphocytes in panglioma remain unknown. In the present study, we systematically explored and validated the expression and prognostic value of BTN2/3 subfamily members in panglioma (TCGA-GBMLGG merge cohort) using multiple public databases. We used clinical specimens for high-throughput verification and cell lines for qRT–PCR verification, which confirmed the expression profiles of BTN2/3 subfamilies. In addition, the function of the BTN2/3 subfamily members and the correlations between BTN2/3 subfamily expression and panglioma immune infiltration levels were investigated. We found that BTN2/3 subfamily members were rarely mutated. BTN2/3 subfamilies were overexpressed in panglioma; high expression of BTN2/3 subfamily members was correlated with poor prognosis. In addition, BTN2/3 subfamilies might positively regulate proliferation, and the overexpression of BTN2/3 subfamilies influenced cell cycle, differentiation, and glioma stemness. In terms of immune infiltrating levels, BTN2/3 subfamily expression was positively associated with CD4+ T cell, B cell, neutrophil, macrophage, and dendritic cell infiltrating levels. These findings suggest that BTN2/3 subfamily expression is correlated with prognosis and immune infiltration levels in glioma. Therefore, the BTN2/3 subfamilies can be used as biomarkers for panglioma and prognostic biomarkers for determining the prognosis and immune infiltration levels in panglioma.

Keywords: BTN/BTNL; BTN2/3; panglioma; immune infiltration