Front. Oncol.Frontiers in OncologyFront. Oncol.2234-943XFrontiers Media S.A.10.3389/fonc.2021.666017OncologyReviewNanozymes in Tumor TheranosticsMaQiulian1†LiuYanfang2†ZhuHaitao1*ZhangLirong1*LiaoXiang13*1Department of Medical Imaging, The Affiliated Hospital of Jiangsu University, Zhenjiang, China2School of Medicine, Jiangsu University, Zhenjiang, China3Department of Laboratory Medicine, The Affiliated Hospital of Jiangsu University, Zhenjiang, China
Edited by: Meihua Yu, University of Queensland, Australia
Reviewed by: Juqun Xi, Yangzhou University, China; Kelong Fan, Institute of Biophysics (CAS), China
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Nanozymes, a new generation of enzyme mimics, have recently attracted great attention. Nanozymes could catalyze chemical reactions as biological enzymes under physiologically mild conditions with higher-efficiency catalytic activities. Moreover, nanozymes could overcome the shortcomings of natural enzymes, such as easy inactivation, high cost, and low yield. With the development of more and more smart and multi-functional nanosystems, nanozymes display great achievement in tumor biology. In this review, we outline the recent advances of nanozymes in tumor and tumor microenvironment diagnosis, therapy, and theranostics.
Despite the great achievement of traditional cancer treatment, such as chemotherapy and immunotherapy, tumors continue to be a major cause of morbidity and mortality. The crosstalk between tumor cells and the tumor microenvironment (TME) is a critical factor for therapy resistance, relapse, and metastasis (1). Therefore, it is important to explore novel strategies to enhance tumor treatment sensitivity by targeting both cancer cells and TME.
Nanomaterials have recently received great interest in enhancing the outcome of cancer therapy, especially nanozymes. Natural enzymes are the proteins or ribonucleic acid (RNA) with highly specific and catalytic ability to their substrates produced by living cells. However, the intrinsic characteristics of natural enzymes, such as storage difficulty, easy deactivation, and high cost, limit their further clinic application (2). With the unexpected discovery of horseradish peroxidase (HRP) activity of Fe3O4 magnetic nanoparticles (Fe3O4 MNPs) in 2007, the artificial nanozymes that display similar catalytic mechanism and efficiency to natural enzymes gradually become research hotspots (3). Nanozymes were firstly identified as nanomaterials possessing intrinsic enzyme-like activities (3). Recently, with the development of chemistry and biology, nanozymes are now termed inorganic or organic nanomaterials possessing intrinsic enzyme-like catalytic activities with abundant advantageous properties compared to natural enzymes, such as lower cost, more facile preparation, higher operational ability, and multi-functionalization (4–7).
Based on the rapid development of nanotechnology, the majority of nanoparticles, such as magnetic nanomaterials, cerium oxide nanoparticles (nanoceria), carbon nanotubes (CNTs), graphene oxide (GO), and gold nanoparticles (Au NPs), have demonstrated their intrinsic redox catalytic activities (3, 8–16). Due to the unique properties of nanozymes and the specific characteristics of tumor and TME, deeper and wider applications of nanozymes in tumor diagnosis, therapy, and theranostics are becoming more and more possible. In this review, we firstly briefly summarize the classification of the most common nanozymes and then discuss the promising applications and challenges of nanozymes in the field of tumor theranostics.
Classification of Nanozymes
Nanozymes mainly include the following subtypes: peroxidase (POD), oxidase (OXD), catalase (CAT), and superoxide dismutase (SOD) (Figure 1). More importantly, great efforts must be devoted to the exploration of novel nanozymes. In this section, we discuss only a few parts of representative nanozymes based on their compositions.
Nanozyme reaction formulas.
Carbon-Based Nanozymes
Carbon-based nanomaterials, including carbon nanotubes (CNTs), graphene oxide (GO), carbon nanospheres, and carbon nanodots (C-Dots), have been proved as the POD mimic catalytic enzyme (11, 15, 17–23), while fullerene and its derivatives perform the SOD-like activity (4).
Combining the ability of hemin to catalyze various oxidation reactions and the large open surface area and rich surface chemistry of graphene, the nanoplatform-modified hemin onto the surface of graphene through the π−π stacking can serve as POD enzymes and display stable geometric support and efficient molecular loading ability (19, 20).
The carboxyl-modified graphene oxide (GO-COOH) with the intrinsic POD property could catalyze the peroxidase substrate 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2) (15). The accompanying blue color reaction makes them capable to be developed for a cheaper and more sensitive glucose detection (15).
CNTs can be distinguished as single-wall carbon nanotubes (SWNTs) and multi-wall carbon nanotubes (MWNTs) according to the number of graphene layers (24). SWNTs could catalyze the substrate of TMB, which have been developed to target dsDNA efficiently (11). Moreover, it has been confirmed that the enzymatic activity of carbon nanotubes strongly depended on pH, temperature, and H2O2 concentration (11).
Based on the superior enzyme activities of nitrogen-doped carbon nanomaterials (N-CNMs), N-doped porous carbon nanospheres (N-PCNSs) possess excellent mimic activities, including OXD-, POD-, CAT-, and POD-like activities (25). These activities are positively correlated with the concentration of N dopant and can also be tunable by pH and temperature (25, 26). Additionally, the B/Fe-doped carbon nanoparticles can also function as POD catalysts (26, 27).
Metal-Based Nanozymes
With the high glucose conversion ability, gold nanoparticles (Au NPs) have been discovered to perform POD- and OXD-like activities (13, 14). Mesoporous silica nanoparticles (MSN) or bovine serum albumin (BSA) can be assembled on the surface of Au NPs for the detection of glucose or dopamine (DA) by the distinguished GOx- and POD-like activities of Au NPs (28, 29). However, high temperature can result in the poor catalytic performance of Au NPs due to the instability of enzymatic product ABTS•+, which can be improved by ionic liquid (30). The stable platinum nanoparticles (Pt NPs) have the ability to scavenge H2O2, superoxide anion (O2−), and singlet oxygen (1O2), simulating CAT-, SOD-, and OXD-like activities. The specific catalytic enzyme activities of Pt NPs are tightly dependent on temperature and pH (31, 32). Under the low pH environment, Pt NPs mostly possess POD-like activity, while Pt NPs exhibit CAT- and SOD-like activities under neutral conditions (31, 32). Moreover, there is a positive correlation between enzyme activities and Pt content. Escapsulating apo-ferritin on the Pt NPs (PtNP@apo-ferritin), this system exhibited more outstanding SOD-like activity and longer-term stability (32, 33).
Metal Oxide-Based Nanozymes
Nanoceria and iron oxide magnetic nanoparticles (Fe3O4 MNPs) are the most widely utilized metal oxide catalysts among the metal oxide-based nanomaterials (5, 8–10, 16, 34, 35). Nanoceria exists in a mixed valence state (Ce3+and Ce4+) (9, 36–38). The ratio of Ce3+ and Ce4+ determines the catalytic enzyme activity of nanoceria. Nanoceria mainly performs SOD-like activity with a high Ce3+/Ce4+ ratio, while performing CAT-mimic activity with a low Ce3+/Ce4+ ratio (7). Moreover, the activity of nanoceria and Fe3O4 MNPs can be controlled by pH. Under the low pH environment, nanoceria possesses an intrinsic OXD-like activity (9). Fe3O4 MNPs display a POD-like activity under acid conditions, while showing CAT-like activity in a neutral environment through the decomposed H2O2 (39–41). The manganic oxide nanoparticles (MnO NPs) behave as the SOD, CAT, and GOx enzymes, inducing the elimination of hydroxyl radical (·OH), maintaining redox homeostasis, and protecting cells from neurotoxin-induced damage (42).
Metal Chalcogenide Nanozymes
Copper monosulfide (CuS) nanoparticles (CuS NPs) have been demonstrated to perform POD-mimic activity by catalyzing the peroxide substrate 3,3’,5,5’-TMB in the presence of H2O2 (43–47). Moreover, with the CuS NPs further covered on the graphene, the CuS-graphene nanosheets (CuS-GNSs) possess higher intrinsic POD- and GOx-like activity than CuS or graphene, respectively, which have been employed to detect H2O2 concentration and monitor the human blood glucose level (44). CuS concave polyhedral superstructures (CuS CPSs) possess superior POD-like activity compared to either the initial formed spherical CuS superstructures or convex CuS microspheres, due to the fact that the concave structures constructed by the thinner nanoplates have a hollow/porous structure that led to a higher surface area (43, 48).
It has been proved that several iron chalcogenides can serve as POD-mimic enzymes. FeS2 nanosheets (FeS2 NSs) possess the ability to oxidate the peroxide substrate TMB due to the Fe ion located in the active site (49). Simultaneously, the peroxidase activity of FeS2 NSs can be tunable by pH and temperature (49). The FeS2/SiO2 double mesoporous hollow spheres (DMHSs) not only exhibit a more outstanding POD-like activity than both Fe3O4 NPs and FeS2 NSs, but also are more susceptive to the detection of H2O2 and glutathione (GSH) (50). The sulfur vacancies in magnetic greigite (SVs-Fe3S4) NSs have demonstrated a distinguished POD-mimic activity resulting from the abundant SVs, which have been developed for the colorimetric detection of glucose in human serum (51).
The MoS2 nanosheets have been developed for the regulation of oxidation stress due to their intrinsic multi-enzyme-like activities under physiological conditions, including SOD-, CAT-, and POD-mimicking activities (52). MoS2 nanosheets can efficiently remove several kinds of reactive oxygen species (ROS) through the Mo6+/Mo4+ redox couple and accelerate the electron transfer between TMB and H2O2 (52).
The reason why nanozymes are considered to have enzyme-like catalytic activities is that they own high catalytic activities and can catalyze the same chemical reactions as biological enzymes. In addition, compared to biological enzymes, nanozymes have superior biocompatibility, stability, and targeting ability, and play corresponding catalytic activities in different environments. The application of nanozymes in tumor diagnosis and treatment depends on their closer integration of nanotechnology and biomedicine for the.
Nanozymes in Tumor Diagnosis, Therapy, and Theranostics
Solid tumors consist of cancer cells and their living environment, also termed tumor microenvironment (TME). Previously, traditional cancer therapy avenues were mostly focused on cancer cells. Recently, more and more lines of evidence have uncovered that the TME is also critical on tumor malignant behaviors. Therefore, targeting both cancer cells and TME is becoming a promising cancer therapy method. TME includes the various soluble substance and stromal cells, such as fibroblasts, immune and inflammatory cells, glial cells, and other cells, as well as nearby micro-vessels and various biological signal molecules (53, 54). The “interactive cooperation” between stromal cells and cancer cells facilitates the progression of tumor and contributes to the dramatic dynamic changes and the heterogeneity of TME (53). In addition, cancer cells could also remodel the TME, ultimately resulting in the immune escape, metastasis, and even relapse of tumor (55). The characterized metabolism manner, rapid growth, and strong reproduction ability of cancer cells determine their higher demands for oxygen and glucose than normal cells. Cancer cells compete with stromal cells to take advantage of glucose for aerobic glycolysis. Also, abundant lactic acid secreted into the extracellular environment ultimately forms the acid and immunosuppressed TME (56–58). The broken balance between oxygen consumption and supply resulted in the messy growth and irregular distribution of tumor vasculature systems, which, in turn, eventually enhanced the degree of permanent or temporary hypoxia and further increased the osmotic pressure of TME (59). Therefore, low pH, hypoxia, excessive H2O2 and GSH, high osmotic pressure, and immunosuppressive microenvironment are the outstanding hallmarks of solid tumors (Figure 2) (53, 56, 58). These characteristics are mutually causal, finally contributing to the rapid progression of tumor. Targeting and normalizing TME seem to be a new and effective method for tumor diagnosis and treatment. Recently, more and more nanozymes have been constructed to target the diagnosis and treatment of TME.
The characteristic of tumor microenvironment.
Nanozymes in Tumor Cells and Tumor Microenvironment Target Diagnosis
Nanotechnology-based tumor target diagnosis and therapy include passive target and active target. Active target greatly relies on the recognition of the specific receptors overexpressed on cancer cells and the ligand-directed binding on the surface of nanosystems (60). Loaded with special markers on the cancer cell surface, such as transferrin, growth factors, peptides, folate, antibodies, or antibody fragments, nanozyme systems not only recognize tumor more sensitively, but also result in drug delivery more specifically (61). The nanozymes modified with folic acid can actively target the folic acid receptors on the cancer cell surface and further can serve as oxidants to promote cancer cell death (9, 17). Porous platinum nanoparticles on graphene oxide (Pt NPs/GO) can function as peroxidase mimetics. which enable them to detect cancer cells by the color reaction of TMB (62). Furthermore, by loading folic acid on the Pt NPs/GO, this nanosystem can distinguish a total of 125 cancer cells more broadly than naked-eye observation (62). Prostate-specific antigen (PSA), a special tumor biomarker, can be attached by the immune complexes based on the intrinsic POD-like activity of GO, and then the PSA concentration could be directly detected with the colorimetric reaction (63). Ultra-small gold nanoclusters (Au NCs) can serve as POD-like catalysts for disproportionation and decomposition of H2O2, which make them sensitive probes for tumor imaging in vivo (64). The multifunctional protease nanosensor constructed by Au NCS not only can determine whether the tissue is cancerous through the catalyzed reaction of Au NCS according to the color reaction, but also is non-toxic and can be completely eliminated by liver and kidney excretion (64). Furthermore, the magneto-ferritin nanoparticles (M-HFn) are composed of iron oxide and heavy-chain ferritin (HFn) shell (65). Due to the ability of targeting transferrin receptor 1 (TfR1) overexpressed on cancer cell surface and the color reaction in tumor site resulting from the POD-like activity of the iron oxide core that could catalyze the abundant H2O2 in TME, M-HFn could visualize cancer tissues sensitively and specifically (65). Similarly, HFn-N-PCNSs-3 can also specifically identify the cancer cells and effectively reduce the tumor volume dependent on the special binding of TfR1 on cancer cell surface and the multi-enzyme mimic activities of N-PCNSs-3 (25). Besides, angiopep-2, a specific ligand of lipoprotein related protein-1 (LRP1), anchored on the surface of Au NPs can penetrate through the blood–brain barrier (BBB) and further actively target glioma cancer cells (66, 67).
Magnetic resonance imaging (MRI) contrast agents based on the ROS-stimulated responses, such as superoxide ions, H2O2, and hydroxyl radicals, have been promising tumor diagnostic and imaging markers due to their extensive accumulation and persistent presence in TME (68–70). Prussian Blue nanoparticles (PBNPs, KFe3+[Fe2+(CN)6]) perform CAT-like activities under the neutral pH condition (71). The core Fe3+ with water coordination can form paramagnetic oxygen bubbles, which are conducive to shorten the MRI T1-weighted image (T1WI) relaxation time and then enhance the MRI contrast (72). Based on the previous pioneering work of PBNPs, SPIO@GCS/acryl/biotin-CAT/SOD-gel (SGC), a dual-enzyme-loaded multifunctional hybrid nanogel probe, has been developed to strengthen the ultrasound imaging and the imaging contrast of T2WI (73). In recent years, the PB@Au core-satellite nanoparticles (CSNPs) have been constructed to explore multiple diagnostic and therapeutic strategies of tumors (72). CSNPs can achieve dual-model imaging due to the PB NPs that acted as MRI T1WI contrast agents and the enhanced computed tomography (CT) imaging efficiency by AuNPs (72). Besides, the MnO NPs exposed to the superoxide radicals could enhance the MRI signal and simultaneously treat the catalytic-induced tumor progression due to their intrinsic SOD-mimic ability (74). Moreover, the CAT-like nanoparticles are gradually utilized as coupling or contrast agents of ultrasound (US) and MRI owing to the enhanced catalyzed H2O2 into O2 molecules (71–73).
Early diagnosis of tumor makes it possible to obtain outstanding tumor clearance and satisfactory clinical prognosis by local treatment. Nowadays, the early detection of tumor mainly depends on the blood tumor markers and imaging manifestations. However, the extremely low abscission rate of early tumor markers or the lack of specificity of imaging findings limits the accuracy and sensitivity of early tumor detection in clinical. The emergence of nanozymes provides new ideas and methods for the early diagnosis of tumor and the visualization of tumor tissues, which greatly improve the specificity and sensitivity of early diagnosis of tumor.
Nanozymes in Synergistic Tumor Therapy
Nanozymes can achieve anti-tumor effects by improving TME. For example, the highly ordered MnO2@PtCo nanoflowers are developed as a ROS generation nanoplatform for tumor therapy by targeting the hypoxia and the acidic pH of TME (75, 76). Cooperating with the OXD-like activity of PtCo and the CAT-like activity of MnO2, the MnO2@PtCo nanozymes not only could supply O2 to overcome the hypoxic TME, but also catalyze ROS formation, which further induces the admirable tumor apoptosis (75). Similarly, the DMSN-Au-Fe3O4 composited nanoplatforms could make the TME-responsive tumor vanish owing to the GOx-mimic activity of Au NPs and the POD-like activity of the Fe3O4 nanoparticles. The DMSN-Au-Fe3O4 nanozymes are capable of catalyzing β-D-glucose oxidated into gluconic acid and subsequently produce high-toxic hydroxyl radicals for tumor regression (41). Based on the abundant GSH detained in TME, pyrite nanozymes and FeS2 with ultrahigh H2O2 affinity promote the glutathione oxidation due to their OXD-like activity and the generation of •OH by their POD-like activity, resulting in the ferroptosis and apoptosis of tumor cells consequently (35). Recently, a novel nanosystem, polyethylene glycol (PEG)-ylated iron manganese silicate nanoparticles (IMSN) loaded with TGF-β inhibitor (TI) (IMSN-PEG-TI), has also been constructed to regulate the tumor immune microenvironment and advance the tumor therapeutic modality through the intrinsic POD- and CAT-like activities of IMSN nanozymes under the acidic TME (58).
Additionally, nanozymes can synergistically enhance the anti-tumor effects of tumor therapy avenues that deeply depend on the oxygen level, such as photodynamic therapy (PDT), photothermal therapy (PTT), sonodynamic therapy (SDT), radiotherapy (RT), and chemotherapy (1, 77). Moreover, consumption of O2 and tumor vasoconstriction can further exacerbate hypoxia and limit the efficiency of the above tumor therapies, which finally form a positive feedback (78–81). Nanozymes are used more and more widely in enhancing the efficiency of these therapies.
Nanozymes in Synergistic Phototherapy
Phototherapy relies on light radiation to induce the death of cancer cells, including PDT and PTT. PDT firstly transforms light energy to the surrounding O2 and then produces a high concentration of cytotoxicity of ROS to further oxidize biomacromolecules and induces their dysfunction (77, 82–84). PTT induces the death of tumor cells depending on the local thermal damage (77, 84, 85). Although the photosensitizers and photothermal agents could enhance therapy efficiency and reduce the side effect of PDT and PTT under near-infrared (NIR) laser irradiation, they convert to excited single states and then return to the ground states by collisions between surrounding molecules. Accompanied by the increased kinetic energy, they consequently result in the heating of the surrounding microenvironment (77, 85).
Although the metal-organic frameworks (MOFs) assembled with photosensitizers can induce the death of tumor cells by the conversion of oxygen into 1O2, the efficiency of PDT is still limited owing to the hypoxia of the TME (80, 81, 86). The novel Pt nanozymes have been placed on the photosensitizers integrated with MOFs to break the limitation of hypoxia. This nanoplatform not only possesses higher stability, but also performs CAT-like activity leading to the additional 1O2 formation and further enhancing the efficiency of PDT (81).
CAT-mimicking Pt NPs are sandwiched into the dual-nanozyme-engineered porphyrin metal organic frameworks (PCN); furthermore, the outer GOx-mimicking Au NPs coordinate with folic acid (Pt@P-Au-FA) (87). The Pt@P-Au-FA NPs can enhance O2 generation by catalyzing H2O2, which further enhances PDT efficiency. What is more, Au NPs strengthen the depletion of glucose and the self-produced H2O2 serve as substrates of Pt NPs, cooperating with glucose depletion-induced starving therapy and achieving remarkable anti-tumor effects (87).
The nanozyme PEG/Ce-Bi@DMSN is constructed by dendritic mesoporous silica coated with uniform Bi2S3 nanorods (Bi2S3@DMSN) and further by ultrasmall ceria placed into the large mesopores of Bi2S3@DMSN, which possesses dual mimic catalytic activities (including POD- and CAT-mimic activities) under primary acidic TME resulting in elevated oxidative stress and relieved hypoxia (88) (Figure 3). Additionally, PEG/Ce-Bi@DMSN allowed the enhanced GSH consumption to be overexpressed in TME. The NIR laser irradiation could strengthen the catalytic activities and GSH depletion of PEG/Ce-Bi@DMSN nanozymes, which further synergistically enhance the tumor ablation effect of PTT (88).
Schematic illustration of the PEG/Ce-Bi@DMSN nanozymes enhancing the efficiency of PTT. Reproduced with permission (88). Copyright 2020, Wiley-VCH.
The platinum-doped Prussian blue (PtPB) nanozyme was developed to improve the photothermal property in a large wavelength range during the process of PTT (89). On the other hand, the PtPB nanozyme is endowed with superior CAT and SOD-like catalytic activities by Pt doped with PB nanotubes, which contributed to the relieved inflammation caused by PTT, along with significant tumor inhibition (89).
Nanozymes in Synergistic Sonodynamic Therapy
Ultrasound (US)-triggered sonodynamic therapy (SDT) consisting of a low-intensity ultrasound and a chemotherapeutic agent (sonosensitizer) is a promising alternative tumor therapeutic modality (90–93). US not only is more accessible and noninvasive in reaching deep-seated tumor tissues, but also can activate sonosensitizers to produce toxic ROS molecules for tumor eradication (90, 91, 94). However, the therapeutic efficiency of SDT is still restricted by severe hypoxia in TME to a great extent (90, 95).
The hollow Pt-CuS Janus can overcome the hypoxia environment due to the mimetic enzyme activity of Pt that decomposes the endogenous overexpressed H2O2 into O2 (47). The hollow Pt-CuS Janus has superior photothermal performance, which not only elevates the Pt enzyme activity for O2 production, but also augments the SDT-induced tumor cell death by higher ROS level simultaneously (47). Hence, the synergistic efficiency of PTT and the catalysis-improved SDT can achieve complete tumor elimination.
The nanoprobe (CDP@HP-T), constructed by Pt-embedded hollow polydopamine (P@HP) nanoparticle, co-loaded with doxorubicin (DOX) and chlorine e6 (Ce6) and further modified with the mitochondrial-targeting molecule triphenyl phosphonium (TPP), can be used to achieve enhanced combination therapy of chemotherapy and SDT for tumors (94). As a pH-responsive nanoprobe, the CDP@HP-T could realize the abundant O2 generation and alleviate the hypoxia of tumor sites responsible for the CAT-like activity of Pt and endogenous overexpressed H2O2 under weakly acidic TME, which further enhances the efficacy of SDT (94). Concomitantly, with DOX and TPP, this nanoprobe could achieve tumor eradication by inhibiting cellular DNA replication, further enhancing the combined therapeutic efficacy of chemotherapy and SDT (94).
Analogously, the ultrafine titanium monoxide (TiO1+x) nanorods modified with PEG (PEG-TiO1+x NRs) enable higher tumor elimination outcome in synergistic chemotherapy and SDT (Figure 4) (96). The TiO1+x NRs possess POD-like activity for the decomposition of H2O2 in TME (96). Notably, the PEG-TiO1+x NRs could generate superior US-induced ROS due to the oxygen-deficient structures within TiO. On the other hand, the PEG-TiO1+x NRs could serve as Fenton-like agents for ROS generation in the presence of Ti3+ (96).
Schematic illustration of PEG-TiO1+x NRs that served as sonosensitizers in the synergistic chemotherapy and SDT. Reproduced with permission (96). Copyright 2020, American Chemical Society.
Nanozymes in Synergistic Radiotherapy
Radiotherapy (RT) has been widely used as the first-line treatment modality of various solid tumors in clinics (97–101). However, the TME complex leads to the ultimate resistance to RT and even the recurrence and metastasis of tumors (98). Normalizing the TME to enhance the effectiveness of RT, to improve hypoxia and increase intratumoral oxygen concentration, and to further promote radiation-induced DNA damage is one of the most common strategies.
Several nanomaterials are designed to enhance tumor radiation sensitivity and attenuate hypoxia by catalyzing the generation of O2 (102, 103). The high reactivity, stability, and specificity of the albumin complex and MnO2 NPs (A-MnO2 NPs) towards H2O2 could simultaneously modulate hypoxia and acidosis TME with regulated pH (102). Furthermore, A-MnO2 NPs could normalize tumor blood vessels by the downregulated hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) (102). Taking advantage of the engineered multifunctional A-MnO2 NPs, the tumor response to radiation can be enhanced significantly (102).
Based on the perfect RT responsiveness of MnO2, gold and manganese dioxide (Au@MnO2) core-shell nanoparticles coated with PEG formed Au@MnO2-PEG (104). Au@MnO2-PEG, using the Au core, functioned as a RT sensitizer and MnO2 shell as CAT mimics that mediate the decomposed H2O2 could not only overcome tumor hypoxia but also enhance the tumor sensitivity to RT (75, 104). More importantly, the Au@MnO2-PEG displays more satisfactory tumor inhibition than the outcome of Au-PEG or MnO2-PEG and has good biocompatibility and biosecurity (104).
Nanozymes in Synergistic Chemotherapy
Chemotherapy, as the most commonly applied cancer treatment modality, induces cancer cell death partly through regulating the formation of ROS (105). Abundant nanozymes loading chemotherapy drugs have been applied in tumor treatment depending on enhancing the generation of ROS (106).
Iron oxide nanoparticles (IONPs), with the POD-like activity, could decompose H2O2 into hydroxyl radicals under acidic or neutral conditions (73). The super-paramagnetic iron oxide nanoparticles (SPION) with inherent POD-like activity are proved to reduce H2O2 in human mesenchymal stem cells (hMSCs) in a dose-dependent manner, and further promote cell proliferation and growth (107). SPION can also be degraded in lysozymes and produce iron ions, which further accelerates the process of cell cycle (106). In addition, the combination of SPION with β-lapachone, an anticancer drug, significantly enhances the intracellular ROS levels and tumor-killing efficiency in non-small cell lung cancers (NSCLCs) (106).
Hollow Ru@CeO2 yolk shell nanozymes (Ru@CeO2 YSNs) loaded with anti-tumor drug ruthenium complex (RBT) and resveratrol (Res), and then modified with PEG, formed the Ru@CeO2-RBT/Res-PEG nanozyme system (108). Ru@CeO2-RBT/Res-DPEG could achieve oxygen supply in situ and enhance the anti-tumor responses of both chemotherapy and PDT. Moreover, it can also limit the metastasis and recurrence of tumors (108).
Nanozyme Systems for Tumor Theranostics
Based on the development of nanotechnology, more and more multi-functional nanozyme platforms are designed, with the ability of multi-model therapy, multi-model imaging, or simultaneously realizing tumor diagnosis and therapy.
The novel PtFe@Fe3O4 nanozyme, with outstanding POD- and CAT-like activities in the acid TME, could overcome the hypoxia in tumor and enhance the effects of PDT. Moreover, PtFe@Fe3O4 could be used as MRI T1WI negative contrast agents (41, 109). The biomimetic hybrid nanozyme (rMGB), integrated with GOx and MnO2, could realize the self-catalytic reaction products under TME stimulation, resulting in enhanced O2 generation and improving the efficiency of starvation therapy and PDT (110). Also, rMGB could be used as the MRI T1WI contrast agents.
Based on the POD-like and ROS-regulated activity of Au NPs under an acid environment, the carbon–gold hybrid (OMCAPs@rBSA-FA@IR780) nanoprobes not only reveal excellent tumor-targeting imaging ability, but also offer outstanding tumor therapeutic performance (111). Besides, the Au2Pt-PEG-Ce6 nanoplatform was developed through Ce6 linked to Au2Pt nanozymes covalently (112). Contributing to the photosensitive characteristics of Ce6 and the dual CAT- and POD-like activities simultaneously of Au2Pt nanozymes, this nanosystem not only can relieve tumor hypoxia with O2 generation but also enhance the efficiency of PDT and chemotherapy with the produced ∙OH (112). Moreover, due to high-Z elements of Au and Pt, Au2Pt-PEG-Ce6 can be possible imaging contrast agents of CT (112, 113).
With single-atom Ru incorporated into the Mn3[Co(CN)6]2 MOF framework, followed by the biocompatible poly-vinylpyrrolidone (PVP) polymer further encapsulating organic ligand, metal ions, and photosensitized Ce6, the self-assembled single-atom enzyme (OxgeMCC-rSAE) was constructed (114). As Ru served as an endogenous oxygen-generating single-atom catalytic site, OxgeMCC-rSAE can degrade H2O2 to generate oxygen, which further enhances the generation of ROS, ultimately enhancing PTT-induced cancer cell death (114). Meanwhile, due to the higher loading of the photosensitizer Ce6, the nanoparticles can selectively aggregate and be visualized in the tumor area by MRI (114).
Conclusion and Prospects
Since the hallmark ferromagnetic nanoparticles proved to be of use as POD natural enzymes in 2007, nanozymes have attracted unprecedented attention and applications, especially in oncology. Although nanozymes have achieved excellent progress in many areas, there are still many problems that cannot be ignored. Currently, most of the present nanozymes mainly focus on the activity of oxidoreductase and hydrolase activities, but the other enzyme activities such as transferase and lyase are still poorly understood. Therefore, it is necessary to explore new nanozyme materials and study their catalytic properties in depth. In addition, the catalytic mechanism of nanozymes is diverse and regulated by various factors. Moreover, different nanozymes may have a synergistic effect in the anti-tumor process. Therefore, it is necessary to establish completeness for different types of nanozyme catalytic systems. The current catalytic efficiency of nanozymes makes it hard to reach the level of natural enzyme in vivo, and their activities are still limited due to the complicated TME. Besides, the poor substrate selectivity of nanozymes persists. Modification of certain specific molecules with nanozymes may solve the problem and may improve the substrate specificity and target the tumor more sensitively and specifically. In addition, studies of nanozymes in tumor theranostics are still in the primary stage. The inherent toxicity and clearance rate of the materials also limit their wide applications. Moreover, various nanozymes have their own unique advantages and shortcomings. Therefore, constructing a nanosystem with good biocompatibility, high targeting efficiency, and multiple functions would be a crucial task.
With the continuous development of nanoscale science and technology, nanozymes show superior versatility, operability, and applicability, thus paving the way for new principles and technologies in disease diagnosis and treatment as well as efficient and precise new nanodrug applications in the biomedical field (Table 1).
Nanozyme Classification and Applications.
Nanozyme system
Mimetic activities
Applications
References
Carbon-Based
SWNTs
POD
Drug delivery; Human SNP DNA detection
(11, 24)
GO
POD
Tumor visual detection
(63)
GO-COOH
GOx; POD
Glucose detection
(15)
GFH
POD
Tumor detection
(17)
C-Dots
POD
Glucose detection
(22)
HFn-N-CNMs-3
POD; OXD (acidic pH values)
Tumor catalytic therapy
(25)
SOD; CAT (neutral pH values)
Anti-oxidant therapy
(25)
Metal-Based
Au NPs
GOx
Self-limiting nanomedicine; Biomedical probe
(14, 111)
EMSN-AuNPs
GOx; POD
Self-activated cascade catalysis
(28)
AuNCs
POD
Tumor detection
(64)
BSA-AuNCs
POD
A dual fluorometric and colorimetric sensor for dopamine
(29)
Au/SiO2 nanocomposites
POD
Realizing high-temperature catalytic reactions
(30)
AuNCs-NH2
CAT
Enhancing PDT efficiency
(83)
Carbon-gold hybrid nanoprobes
CAT
Real-time imaging, enhancing PTT and PDT efficiency
Detection of organophosphorus pesticide and nerve agent
(3, 16, 39)
CAT (neutral pH values)
Anti-oxidant
(39)
PtFe@Fe3O4
POD; CAT
Synergistic tumor catalytic therapy and PTT
(109)
DMSN-Au-Fe3O4 NPs
POD; GOx
Anti-tumor
(41)
Nanoceria
SOD (neutral pH values)
Against radiation damage, oxidative stress and inflammation
(9, 10, 38)
Fluorogenic detection of cancer
Folate-conjugated Nanoceria
OXD (acidic pH values)
Cancer detection
(9)
PEG-CNPS
SOD
Radical scavenger with tunable redox chemistry
(36)
A-MnO2 NPs
CAT
Modulating TME and enhancing RT responses
(102)
MnO NPs
SOD
Enhancing MRI contrast
(74)
Au@MnO2-PEG
CAT
Enhanced RT via improving the tumor oxygenation
(104)
Mn3O4 NPs
SOD; CAT; GPx
Anti-inflammation
(76)
MnO2@PtCo
OXD; CAT
Anti-tumor
(75)
rMGB
CAT
Enhancing starvation and PDT against hypoxic tumor
(110)
Ru@CeO2-RBT/Res-DPEG
CAT
Enhancing dual chemotherapy combined with PTT
(108)
PEG-TiO1+x NRs
POD
Enhancing dual chemotherapy combined with SDT
(96)
Metal Chalcogenide
CuS-GNSs
POD; GOx
Detection of H2O2 and human serum glucose level
(44)
Hollow Pt-CuS Janus
POD
Synergistic PTT and SDT
(47)
DMHSs-FeS2/SiO2
POD
Detection of H2O2 and GSH for anti-tumor
(50)
SVs-Fe3S4
POD
Detection of human serum glucose level
(51)
FeS2
OXD; POD
Anti-tumor
(35)
PEG/Ce-Bi@DMSN
POD; CAT
Synergistic tumor catalytic therapy and PTT
(88)
Author Contributions
QM and YL wrote the draft of the manuscript. HZ and LZ contributed to the conception of the work and organized the structure of the manuscript. XL performed the revision. All authors contributed to the article and approved the submitted version.
Funding
This work was supported by grants from the National Natural Science Foundation of China (grant number 82071984), the Young Medical Talents of Jiangsu (grant number QNRC2016833), the Six Talent Peaks Project of Jiangsu Province (grant number WSW-039), the Six for One Project of Jiangsu Province (grant number LGY2018093), the Project of Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX19_0577), and the Social Development Foundation of Zhenjiang City (grant number SH2021071).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
ReferencesBarkerHEPagetJTKhanAAHarringtonKJ. The Tumour Microenvironment After Radiotherapy: Mechanisms of Resistance and Recurrence. Nat Rev Cancer (2015) 15(7):409–25. doi: 10.1038/nrc3958LiangMMYanXY. Nanozymes: From New Concepts, Mechanisms, and Standards to Applications. Acc Chem Res (2019) 52(8):2190–200. doi: 10.1021/acs.accounts.9b00140GaoLZhuangJNieLZhangJZhangYGuN. Intrinsic Peroxidase-Like Activity of Ferromagnetic Nanoparticles. Nat Nanotechnol (2007) 2(9):577–83. doi: 10.1038/nnano.2007.260WeiHWangEK. Nanomaterials With Enzyme-Like Characteristics (Nanozymes): Next-Generation Artificial Enzymes. Chem Soc Rev (2013) 42(14):6060–93. doi: 10.1039/c3cs35486eLinYHRenJSQuXG. Nano-Gold as Artificial Enzymes: Hidden Talents. Adv Mater (2014) 26(25):4200–17. doi: 10.1002/adma.201400238JiangBDuanDMGaoLZZhouMJFanKLTangY. Standardized Assays for Determining the Catalytic Activity and Kinetics of Peroxidase-Like Nanozymes. Nat Protoc (2018) 13(7):1506–20. doi: 10.1038/s41596-018-0001-1WuJJXWangXYWangQLouZPLiSRZhuYY. Nanomaterials With Enzyme-Like Characteristics (Nanozymes): Next-Generation Artificial Enzymes (II). Chem Soc Rev (2019) 48(4):1004–76. doi: 10.1039/c8cs00457aParkKSKimMIChoDYParkHG. Label-Free Colorimetric Detection of Nucleic Acids Based on Target-Induced Shielding Against the Peroxidase-Mimicking Activity of Magnetic Nanoparticles. Small (2011) 7(11):1521–5. doi: 10.1002/smll.201001886AsatiASantraSKaittanisCNathSPerezJM. Oxidase-Like Activity of Polymer-Coated Cerium Oxide Nanoparticles. Angew Chem Int Ed Engl (2009) 48(13):2308–12. doi: 10.1002/anie.200805279HeckertEGKarakotiASSealSSelfWT. The Role of Cerium Redox State in the SOD Mimetic Activity of Nanoceria. Biomaterials (2008) 29(18):2705–9. doi: 10.1016/j.biomaterials.2008.03.014SongYJWangXHZhaoCQuKGRenJSQuXG. Label-Free Colorimetric Detection of Single Nucleotide Polymorphism by Using Single-Walled Carbon Nanotube Intrinsic Peroxidase-Like Activity. Chemistry (2010) 16(12):3617–21. doi: 10.1002/chem.200902643PengoPPolizziSPasquatoLScriminP. Carboxylate-Imidazole Cooperativity in Dipeptide-Functionalized Gold Nanoparticles With Esterase-Like Activity. J Am Chem Soc (2005) 127(6):1616–7. doi: 10.1021/ja043547cComottiMDella PinaCMatarreseRRossiM. The Catalytic Activity of “Naked” Gold Particles. Angew Chem Int Ed Engl (2004) 43(43):5812–5. doi: 10.1002/anie.200460446LuoWJZhuCFSuSLiDHeYHuangQ. Self-Catalyzed, Self-Limiting Growth of Glucose Oxidase-Mimicking Gold Nanoparticles. ACS Nano (2010) 4(12):7451–8. doi: 10.1021/nn102592hSongYJQuKGZhaoCRenJSQuXG. Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and its Application to Glucose Detection. Adv Mater (2010) 22(19):2206–10. doi: 10.1002/adma.200903783LiangMMFanKLPanYJiangHWangFYangDL. Fe3O4 Magnetic Nanoparticle Peroxidase Mimetic-Based Colorimetric Assay for the Rapid Detection of Organophosphorus Pesticide and Nerve Agent. Anal Chem (2013) 85(1):308–12. doi: 10.1021/ac302781rSongYJChenYFengLYRenJSQuXG. Selective and Quantitative Cancer Cell Detection Using Target-Directed Functionalized Graphene and its Synergetic Peroxidase-Like Activity. Chem Commun (Camb) (2011) 47(15):4436–8. doi: 10.1039/c0cc05533fTaoYLinYHRenJSQuXG. Self-Assembled, Functionalized Graphene and DNA as a Universal Platform for Colorimetric Assays. Biomaterials (2013) 34(20):4810–7. doi: 10.1016/j.biomaterials.2013.03.039XueTJiangSQuYQSuQChengRDubinS. Graphene-Supported Hemin as a Highly Active Biomimetic Oxidation Catalyst. Angew Chem Int Ed Engl (2012) 51(16):3822–5. doi: 10.1002/anie.201108400GuoYDengLLiJGuoSWangEDongS. Hemin-Graphene Hybrid Nanosheets With Intrinsic Peroxidase-Like Activity for Label-Free Colorimetric Detection of Single-Nucleotide Polymorphism. ACS Nano (2011) 5(2):1282–90. doi: 10.1021/nn1029586SunHJZhouYRenJSQuXG. Carbon Nanozymes: Enzymatic Properties, Catalytic Mechanism, and Applications. Angew Chem Int Ed Engl (2018) 57(30):9224–37. doi: 10.1002/anie.201712469ShiWBWangQLLongYJChengZLChenSHZhengHZ. Carbon Nanodots as Peroxidase Mimetics and Their Applications to Glucose Detection. Chem Commun (Camb) (2011) 47(23):6695–7. doi: 10.1039/c1cc11943eZhengXXLiuQJingCLiYLiDLuoWJ. Catalytic Gold Nanoparticles for Nanoplasmonic Detection of DNA Hybridization. Angew Chem Int Ed Engl (2011) 50(50):11994–8. doi: 10.1002/anie.201105121LiuZTabakmanSMChenZDaiHJ. Preparation of Carbon Nanotube Bioconjugates for Biomedical Applications. Nat Protoc (2009) 4(9):1372–82. doi: 10.1038/nprot.2009.146FanKLXiJQFanLWangPXZhuCHTangY. In Vivo Guiding Nitrogen-Doped Carbon Nanozyme for Tumor Catalytic Therapy. Nat Commun (2018) 9(1):1440. doi: 10.1038/s41467-018-03903-8HuangYYRenJSQuXG. Nanozymes: Classification, Catalytic Mechanisms, Activity Regulation, and Applications. Chem Rev (2019) 119(6):4357–412. doi: 10.1021/acs.chemrev.8b00672JiangXXLiuKLiQLiuMYangMHChenX. B-Doped Core-Shell Fe@BC Nanozymes: Active Site Identification and Bacterial Inhibition. Chem Commun (Camb) (2021) 57(13):1623–6. doi: 10.1039/d0cc06692cLinYHLiZHChenZWRenJSQuXG. Mesoporous Silica-Encapsulated Gold Nanoparticles as Artificial Enzymes for Self-Activated Cascade Catalysis. Biomaterials (2013) 34(11):2600–10. doi: 10.1016/j.biomaterials.2013.01.007TaoYLinYHRenJSQuXG. A Dual Fluorometric and Colorimetric Sensor for Dopamine Based on BSA-Stabilized Au Nanoclusters. Biosens Bioelectron (2013) 42:41–6. doi: 10.1016/j.bios.2012.10.014LinYHZhaoADTaoYRenJSQuXG. Ionic Liquid as an Efficient Modulator on Artificial Enzyme System: Toward the Realization of High-Temperature Catalytic Reactions. J Am Chem Soc (2013) 135(11):4207–10. doi: 10.1021/ja400280fLiuYWuHHLiMYinJJNieZH. pH Dependent Catalytic Activities of Platinum Nanoparticles With Respect to the Decomposition of Hydrogen Peroxide and Scavenging of Superoxide and Singlet Oxygen. Nanoscale (2014) 6(20):11904–10. doi: 10.1039/c4nr03848gFanJYinJJNingBWuXCHuYFerrariM. Direct Evidence for Catalase and Peroxidase Activities of Ferritin-Platinum Nanoparticles. Biomaterials (2011) 32(6):1611–8. doi: 10.1016/j.biomaterials.2010.11.004ZhangLBLaugLMünchgesangWPippelEGöseleUBrandschM. Reducing Stress on Cells With Apoferritin-Encapsulated Platinum Nanoparticles. Nano Lett (2010) 10(1):219–23. doi: 10.1021/nl903313rKotovNA. Chemistry. Inorganic Nanoparticles as Protein Mimics. Science (2010) 330(6001):188–9. doi: 10.1126/science.1190094MengXQLiDDChenLHeHLWangQHongCY. High-Performance Self-Cascade Pyrite Nanozymes for Apoptosis-Ferroptosis Synergistic Tumor Therapy. ACS Nano (2021) 15(3):5735–51. doi: 10.1021/acsnano.1c01248KarakotiASSinghSKumarAMalinskaMKuchibhatlaSVWozniakK. PEGylated Nanoceria as Radical Scavenger With Tunable Redox Chemistry. J Am Chem Soc (2009) 131(40):14144–5. doi: 10.1021/ja9051087AsatiASantraSKaittanisCPerezJM. Surface-Charge-Dependent Cell Localization and Cytotoxicity of Cerium Oxide Nanoparticles. ACS Nano (2010) 4(9):5321–31. doi: 10.1021/nn100816sAsatiAKaittanisCSantraSPerezJM. pH-Tunable Oxidase-Like Activity of Cerium Oxide Nanoparticles Achieving Sensitive Fluorigenic Detection of Cancer Biomarkers at Neutral Ph. Anal Chem (2011) 83(7):2547–53. doi: 10.1021/ac102826kChenZWYinJJZhouYTZhangYSongLNSongMJ. Dual Enzyme-Like Activities of Iron Oxide Nanoparticles and Their Implication for Diminishing Cytotoxicity. ACS Nano (2012) 6(5):4001–12. doi: 10.1021/nn300291rVallabaniNVSVinuASinghSKarakotiA. Tuning the ATP-Triggered Pro-Oxidant Activity of Iron Oxide-Based Nanozyme Towards an Efficient Antibacterial Strategy. J Colloid Interface Sci (2020) 567:154–64. doi: 10.1016/j.jcis.2020.01.099GaoSSLinHZhangHXYaoHLChenYShiJL. Nanocatalytic Tumor Therapy by Biomimetic Dual Inorganic Nanozyme-Catalyzed Cascade Reaction. Adv Sci (Weinh) (2019) 6(3):1801733. doi: 10.1002/advs.201801733SinghNSavanurMASrivastavaSD’SilvaPMugeshG. A Redox Modulatory Mn3O4 Nanozyme With Multi-Enzyme Activity Provides Efficient Cytoprotection to Human Cells in a Parkinson’s Disease Model. Angew Chem Int Ed Engl (2017) 56(45):14267–71. doi: 10.1002/anie.201708573HeWWJiaHMLiXXLeiYLiJZhaoHX. Understanding the Formation of CuS Concave Superstructures With Peroxidase-Like Activity. Nanoscale (2012) 4(11):3501–6. doi: 10.1039/c2nr30310hDuttaAKDasSSamantaSSamantaPKAdhikaryBBiswasP. CuS Nanoparticles as a Mimic Peroxidase for Colorimetric Estimation of Human Blood Glucose Level. Talanta (2013) 107:361–7. doi: 10.1016/j.talanta.2013.01.032YangZJCaoYLiJLuMMJiangZKHuXY. Smart CuS Nanoparticles as Peroxidase Mimetics for the Design of Novel Label-Free Chemiluminescent Immunoassay. ACS Appl Mater Interfaces (2016) 8(19):12031–8. doi: 10.1021/acsami.6b02481SwaidanAAddadATahonJFBarrasAToufailyJHamiehT. Ultrasmall CuS-BSA-Cu3(PO4)2 Nanozyme for Highly Efficient Colorimetric Sensing of H2O2 and Glucose in Contact Lens Care Solutions and Human Serum. Anal Chim Acta (2020) 1109:78–89. doi: 10.1016/j.aca.2020.02.064LiangSDengXRChangYSunCQShaoSXieZX. Intelligent Hollow Pt-CuS Janus Architecture for Synergistic Catalysis-Enhanced Sonodynamic and Photothermal Cancer Therapy. Nano Lett (2019) 19(6):4134–45. doi: 10.1021/acs.nanolett.9b01595ZhuJLPengXNieWWangYJGaoJWWenW. Hollow Copper Sulfide Nanocubes as Multifunctional Nanozymes for Colorimetric Detection of Dopamine and Electrochemical Detection of Glucose. Biosens Bioelectron (2019) 141:111450. doi: 10.1016/j.bios.2019.111450HuangXNanZD. Porous 2d FeS2 Nanosheets as a Peroxidase Mimic for Rapid Determination of H2O2. Talanta (2020) 216:120995. doi: 10.1016/j.talanta.2020.120995HuangXXiaFNanZD. Fabrication of FeS2/SiO2 Double Mesoporous Hollow Spheres as an Artificial Peroxidase and Rapid Determination of H2O2 and Glutathione. ACS Appl Mater Interfaces (2020) 12(41):46539–48. doi: 10.1021/acsami.0c12593LiuWTianJRMaoCLWangZFLiuJDahlgrenRA. Sulfur Vacancy Promoted Peroxidase-Like Activity of Magnetic Greigite (Fe3S4) for Colorimetric Detection of Serum Glucose. Anal Chim Acta (2020) 1127:246–55. doi: 10.1016/j.aca.2020.06.056ChenTMZouHWuXJLiuCCSituBZhengL. Nanozymatic Antioxidant System Based on MoS2 Nanosheets. ACS Appl Mater Interfaces (2018) 10(15):12453–62. doi: 10.1021/acsami.8b01245HoWJJaffeeEMZhengL. The Tumour Microenvironment in Pancreatic Cancer-Clinical Challenges and Opportunities. Nat Rev Clin Oncol (2020) 17(9):527–40. doi: 10.1038/s41571-020-0363-5BalachandranVPBeattyGLDouganSK. Broadening the Impact of Immunotherapy to Pancreatic Cancer: Challenges and Opportunities. Gastroenterology (2019) 156(7):2056–72. doi: 10.1053/j.gastro.2018.12.038MittalDGubinMMSchreiberRDSmythMJ. New Insights Into Cancer Immunoediting and its Three Component Phases–Elimination, Equilibrium and Escape. Curr Opin Immunol (2014) 27:16–25. doi: 10.1016/j.coi.2014.01.004ShiRCTangYQMiaoHM. Metabolism in Tumor Microenvironment: Implications for Cancer Immunotherapy. MedComm (2020) 1(1):47–68. doi: 10.1002/mco2.6CorbetCFeronO. Tumour Acidosis: From the Passenger to the Driver’s Seat. Nat Rev Cancer (2017) 17(10):577–93. doi: 10.1038/nrc.2017.77XuBLCuiYWangWWLiSSLyuCLWangS. Immunomodulation-Enhanced Nanozyme-Based Tumor Catalytic Therapy. Adv Mater (2020) 32(33):e2003563. doi: 10.1002/adma.202003563JingXMYangFMShaoCCWeiKXieMYShenH. Role of Hypoxia in Cancer Therapy by Regulating the Tumor Microenvironment. Mol Cancer (2019) 18(1):157. doi: 10.1186/s12943-019-1089-9FokongSTheekBWuZKoczeraPAppoldLJorgeS. Image-Guided, Targeted and Triggered Drug Delivery to Tumors Using Polymer-Based Microbubbles. J Control Release (2012) 163(1):75–81. doi: 10.1016/j.jconrel.2012.05.007LeeALWangYChengHYPervaizSYangYY. The Co-Delivery of Paclitaxel and Herceptin Using Cationic Micellar Nanoparticles. Biomaterials (2009) 30(5):919–27. doi: 10.1016/j.biomaterials.2008.10.062ZhangLNDengHHLinFLXuXWWengSHLiuAL. In Situ Growth of Porous Platinum Nanoparticles on Graphene Oxide for Colorimetric Detection of Cancer Cells. Anal Chem (2014) 86(5):2711–8. doi: 10.1021/ac404104jQuFLiTYangM. Colorimetric Platform for Visual Detection of Cancer Biomarker Based on Intrinsic Peroxidase Activity of Graphene Oxide. Biosens Bioelectron (2011) 26(9):3927–31. doi: 10.1016/j.bios.2011.03.013LoynachanCNSoleimanyAPDudaniJSLinYNajerABekdemirA. Renal Clearable Catalytic Gold Nanoclusters for In Vivo Disease Monitoring. Nat Nanotechnol (2019) 14(9):883–90. doi: 10.1038/s41565-019-0527-6FanKLCaoCQPanYXLuDYangDLFengJ. Magnetoferritin Nanoparticles for Targeting and Visualizing Tumour Tissues. Nat Nanotechnol (2012) 7(7):459–64. doi: 10.1038/nnano.2012.90HuangSXLiJFHanLLiuSHMaHJHuangRQ. Dual Targeting Effect of Angiopep-2-Modified, DNA-Loaded Nanoparticles for Glioma. Biomaterials (2011) 32(28):6832–8. doi: 10.1016/j.biomaterials.2011.05.064XinHLJiangXYGuJJShaXYChenLCLawK. Angiopep-Conjugated Poly(Ethylene Glycol)-Co-Poly(ϵ-Caprolactone) Nanoparticles as Dual-Targeting Drug Delivery System for Brain Glioma. Biomaterials (2011) 32(18):4293–305. doi: 10.1016/j.biomaterials.2011.02.044RheeSG. Cell Signaling. H2O2, a Necessary Evil for Cell Signaling. Science (2006) 312(5782):1882–3. doi: 10.1126/science.1130481ZhangKKaufmanRJ. From Endoplasmic-Reticulum Stress to the Inflammatory Response. Nature (2008) 454(7203):455–62. doi: 10.1038/nature07203YangFHuSLZhangYCaiXWHuangYWangF. A Hydrogen Peroxide-Responsive O₂ Nanogenerator for Ultrasound and Magnetic-Resonance Dual Modality Imaging. Adv Mater (2012) 24(38):5205–11. doi: 10.1002/adma.201202367ZhangWHuSLYinJJHeWWLuWMaM. Prussian Blue Nanoparticles as Multienzyme Mimetics and Reactive Oxygen Species Scavengers. J Am Chem Soc (2016) 138(18):5860–5. doi: 10.1021/jacs.5b12070DouYLiXYangWTGuoYYWuMLLiuYJ. PB@Au Core-Satellite Multifunctional Nanotheranostics for Magnetic Resonance and Computed Tomography Imaging In Vivo and Synergetic Photothermal and Radiosensitive Therapy. ACS Appl Mater Interfaces (2017) 9(2):1263–72. doi: 10.1021/acsami.6b13493WangXNiuDCLiPWuQBoXWLiuBJ. Dual-Enzyme-Loaded Multifunctional Hybrid Nanogel System for Pathological Responsive Ultrasound Imaging and T2-Weighted Magnetic Resonance Imaging. ACS Nano (2015) 9(6):5646–56. doi: 10.1021/nn5068094RaggRSchilmannAMKorscheltKWieseotteCKluenkerMVielM. Intrinsic Superoxide Dismutase Activity of MnO Nanoparticles Enhances the Magnetic Resonance Imaging Contrast. J Mater Chem B (2016) 4(46):7423–8. doi: 10.1039/c6tb02078jWangZZZhangYJuEGLiuZCaoFFChenZW. Biomimetic Nanoflowers by Self-Assembly of Nanozymes to Induce Intracellular Oxidative Damage Against Hypoxic Tumors. Nat Commun (2018) 9(1):3334. doi: 10.1038/s41467-018-05798-xYaoJChengYZhouMZhaoSLinSCWangX. ROS Scavenging Mn3O4 Nanozymes for In Vivo Anti-Inflammation. Chem Sci (2018) 9(11):2927–33. doi: 10.1039/c7sc05476aLiXSLovellJFYoonJChenXY. Clinical Development and Potential of Photothermal and Photodynamic Therapies for Cancer. Nat Rev Clin Oncol (2020) 17(11):657–74. doi: 10.1038/s41571-020-0410-2QianCGYuJCChenYLHuQYXiaoXZSunWJ. Light-Activated Hypoxia-Responsive Nanocarriers for Enhanced Anticancer Therapy. Adv Mater (2016) 28(17):3313–20. doi: 10.1002/adma.201505869TongXSrivatsanAJacobsonOWangYWangZTYangXY. Monitoring Tumor Hypoxia Using 18F-FMISO PET and Pharmacokinetics Modeling After Photodynamic Therapy. Sci Rep (2016) 6:31551. doi: 10.1038/srep31551LuKDHeCBGuoNNChanCNiKYWeichselbaumRR. Chlorin-Based Nanoscale Metal-Organic Framework Systemically Rejects Colorectal Cancers via Synergistic Photodynamic Therapy and Checkpoint Blockade Immunotherapy. J Am Chem Soc (2016) 138(38):12502–10. doi: 10.1021/jacs.6b06663ZhangYWangFMLiuCQWangZZKangLHHuangYY. Nanozyme Decorated Metal-Organic Frameworks for Enhanced Photodynamic Therapy. ACS Nano (2018) 12(1):651–61. doi: 10.1021/acsnano.7b07746JiangYYLiJCZengZLXieCLyuYPuKY. Organic Photodynamic Nanoinhibitor for Synergistic Cancer Therapy. Angew Chem Int Ed Engl (2019) 58(24):8161–5. doi: 10.1002/anie.201903968LiuCPWuTHLiuCYChenKCChenYXChenGS. Self-Supplying O2 Through the Catalase-Like Activity of Gold Nanoclusters for Photodynamic Therapy Against Hypoxic Cancer Cells. Small (2017) 13(26):1–9. doi: 10.1002/smll.201700278YangYZhuDMLiuYJiangBJiangWYanXY. Platinum-Carbon-Integrated Nanozymes for Enhanced Tumor Photodynamic and Photothermal Therapy. Nanoscale (2020) 12(25):13548–57. doi: 10.1039/d0nr02800bLiuSPanXTLiuHY. Two-Dimensional Nanomaterials for Photothermal Therapy. Angew Chem Int Ed Engl (2020) 59(15):5890–900. doi: 10.1002/anie.201911477LiuJJYangYZhuWWYiXDongZLXuXN. Nanoscale Metal-Organic Frameworks for Combined Photodynamic & Radiation Therapy in Cancer Treatment. Biomaterials (2016) 97:1–9. doi: 10.1016/j.biomaterials.2016.04.034LiuCXingJAkakuruOULuoLJSunSZouRF. Nanozymes-Engineered Metal-Organic Frameworks for Catalytic Cascades-Enhanced Synergistic Cancer Therapy. Nano Lett (2019) 19(8):5674–82. doi: 10.1021/acs.nanolett.9b02253DongSMDongYSJiaTLiuSKLiuJYangD. GSH-Depleted Nanozymes With Hyperthermia-Enhanced Dual Enzyme-Mimic Activities for Tumor Nanocatalytic Therapy. Adv Mater (2020) 32(42):e2002439. doi: 10.1002/adma.202002439LiZHChenYSunYYZhangXZ. Platinum-Doped Prussian Blue Nanozymes for Multiwavelength Bioimaging Guided Photothermal Therapy of Tumor and Anti-Inflammation. ACS Nano (2021) 15(3):5189–200. doi: 10.1021/acsnano.0c10388SonSBKimJHWangXWZhangCLYoonSAShinJW. Multifunctional Sonosensitizers in Sonodynamic Cancer Therapy. Chem Soc Rev (2020) 49(11):3244–61. doi: 10.1039/c9cs00648fZhuPChenYShiJL. Nanoenzyme-Augmented Cancer Sonodynamic Therapy by Catalytic Tumor Oxygenation. ACS Nano (2018) 12(4):3780–95. doi: 10.1021/acsnano.8b00999LiangSDengXRMaPAChengZYLinJ. Recent Advances in Nanomaterial-Assisted Combinational Sonodynamic Cancer Therapy. Adv Mater (2020) 32(47):e2003214. doi: 10.1002/adma.202003214SunDPangXChengYMingJXiangSJZhangC. Ultrasound-Switchable Nanozyme Augments Sonodynamic Therapy Against Multidrug-Resistant Bacterial Infection. ACS Nano (2020) 14(2):2063–76. doi: 10.1021/acsnano.9b08667AnJHuYGChengKLiCHouXLWangGL. ROS-Augmented and Tumor-Microenvironment Responsive Biodegradable Nanoplatform for Enhancing Chemo-Sonodynamic Therapy. Biomaterials (2020) 234:119761. doi: 10.1016/j.biomaterials.2020.119761LiGZWangSPDengDSXiaoZSDongZLWangZP. Fluorinated Chitosan to Enhance Transmucosal Delivery of Sonosensitizer-Conjugated Catalase for Sonodynamic Bladder Cancer Treatment Post-Intravesical Instillation. ACS Nano (2020) 14(2):1586–99. doi: 10.1021/acsnano.9b06689WangXWZhongXYBaiLXXuJGongFDongZL. Ultrafine Titanium Monoxide (TiO1+x) Nanorods for Enhanced Sonodynamic Therapy. J Am Chem Soc (2020) 142(14):6527–37. doi: 10.1021/jacs.9b10228ZhangCZhaoKLBuWBNiDLLiuYYFengJW. Marriage of Scintillator and Semiconductor for Synchronous Radiotherapy and Deep Photodynamic Therapy With Diminished Oxygen Dependence. Angew Chem Int Ed Engl (2015) 54(6):1770–4. doi: 10.1002/anie.201408472SongGSChengLChaoYYangKLiuZ. Emerging Nanotechnology and Advanced Materials for Cancer Radiation Therapy. Adv Mater (2017) 29(32):1–26. doi: 10.1002/adma.201700996FanWPShenBBuWBChenFZhaoKLZhangSJ. Rattle-Structured Multifunctional Nanotheranostics for Synergetic Chemo-/Radiotherapy and Simultaneous Magnetic/Luminescent Dual-Mode Imaging. J Am Chem Soc (2013) 135(17):6494–503. doi: 10.1021/ja312225bXiaoQFZhengXPBuWBGeWQZhangSJChenF. A Core/Satellite Multifunctional Nanotheranostic for In Vivo Imaging and Tumor Eradication by Radiation/Photothermal Synergistic Therapy. J Am Chem Soc (2013) 135(35):13041–8. doi: 10.1021/ja404985wFanWPBuWBZhangZShenBZhangHHeQJ. X-Ray Radiation-Controlled NO-Release for on-Demand Depth-Independent Hypoxic Radiosensitization. Angew Chem Int Ed Engl (2015) 54(47):14026–30. doi: 10.1002/anie.201504536PrasadPGordijoCRAbbasiAZMaedaAIpARauthAM. Multifunctional Albumin-MnO₂ Nanoparticles Modulate Solid Tumor Microenvironment by Attenuating Hypoxia, Acidosis, Vascular Endothelial Growth Factor and Enhance Radiation Response. ACS Nano (2014) 8(4):3202–12. doi: 10.1021/nn405773rGordijoCRShuhendlerAJWuXY. Glucose-Responsive Bioinorganic Nanohybrid Membrane for Self-Regulated Insulin Release. Adv Funct Mater (2010) 20(9):1404–12. doi: 10.1002/adfm.200901581YiXChenLZhongXYGaoRLQianYTWuF. Core–shell Au@MnO2 Nanoparticles for Enhanced Radiotherapy via Improving the Tumor Oxygenation. Nano Res (2016) 9(11):3267–78. doi: 10.1007/s12274-016-1205-8ZhangCBuWBNiDLZhangSJLiQYaoZW. Synthesis of Iron Nanometallic Glasses and Their Application in Cancer Therapy by a Localized Fenton Reaction. Angew Chem Int Ed Engl (2016) 55(6):2101–6. doi: 10.1002/anie.201510031AliliLSackMKarakotiASTeuberSPuschmannKHirstSM. Combined Cytotoxic and Anti-Invasive Properties of Redox-Active Nanoparticles in Tumor-Stroma Interactions. Biomaterials (2011) 32(11):2918–29. doi: 10.1016/j.biomaterials.2010.12.056LinWSHuangYWZhouXDMaY. Toxicity of Cerium Oxide Nanoparticles in Human Lung Cancer Cells. Int J Toxicol (2006) 25(6):451–7. doi: 10.1080/10915810600959543ZhuXFGongYCLiuYNYangCHWuSJYuanGL. Ru@CeO2 Yolk Shell Nanozymes: Oxygen Supply in Situ Enhanced Dual Chemotherapy Combined With Photothermal Therapy for Orthotopic/Subcutaneous Colorectal Cancer. Biomaterials (2020) 242:119923. doi: 10.1016/j.biomaterials.2020.119923LiSSShangLXuBLWangSHGuKWuQY. A Nanozyme With Photo-Enhanced Dual Enzyme-Like Activities for Deep Pancreatic Cancer Therapy. Angew Chem Int Ed Engl (2019) 58(36):12624–31. doi: 10.1002/anie.201904751YangXYangYGaoFWeiJJQianCGSunMJ. Biomimetic Hybrid Nanozymes With Self-Supplied H+ and Accelerated O2 Generation for Enhanced Starvation and Photodynamic Therapy Against Hypoxic Tumors. Nano Lett (2019) 19(7):4334–42. doi: 10.1021/acs.nanolett.9b00934ZhangAPanSJZhangYHChangJChengJHuangZC. Carbon-Gold Hybrid Nanoprobes for Real-Time Imaging, Photothermal/Photodynamic and Nanozyme Oxidative Therapy. Theranostics (2019) 9(12):3443–58. doi: 10.7150/thno.33266WangMChangMYChenQWangDMLiCXHouZY. Au2Pt-PEG-Ce6 Nanoformulation With Dual Nanozyme Activities for Synergistic Chemodynamic Therapy/Phototherapy. Biomaterials (2020) 252:120093. doi: 10.1016/j.biomaterials.2020.120093LiYYunKHLeeHGohSHSuhYGChoiY. Porous Platinum Nanoparticles as a High-Z and Oxygen Generating Nanozyme for Enhanced Radiotherapy In Vivo. Biomaterials (2019) 197:12–9. doi: 10.1016/j.biomaterials.2019.01.004WangDDWuHHPhuaSZFYangGBQiLWGuL. Self-Assembled Single-Atom Nanozyme for Enhanced Photodynamic Therapy Treatment of Tumor. Nat Commun (2020) 11(1):357. doi: 10.1038/s41467-019-14199-7