AUTHOR=Su Jing , Huang Lynn S. , Barnard Ryan , Parks Graham , Cappellari James , Bellinger Christina , Dotson Travis , Craddock Lou , Prakash Bharat , Hovda Jonathan , Clark Hollins , Petty William Jeffrey , Pasche Boris , Chan Michael D. , Miller Lance D. , Ruiz Jimmy 

TITLE=Comprehensive and Computable Molecular Diagnostic Panel (C2Dx) From Small Volume Specimens for Precision Oncology: Molecular Subtyping of Non-Small Cell Lung Cancer From Fine Needle Aspirates

JOURNAL=Frontiers in Oncology

VOLUME=Volume 11 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.584896

DOI=10.3389/fonc.2021.584896

ISSN=2234-943X

ABSTRACT=We have been pioneering the Comprehensive, Computable NanoString Diagnostic gene panel (C2Dx) as a promising solution to address the need for a molecular pathological research/diagnostic tool for precision oncology utilizing small volume tumor specimens. In this work, through translating knowledge of subtyping-related gene expression patterns learned in our previous work from public transcriptomic data, we established a highly robust, highly accurate lung cancer subtyping system that demonstrated supreme performance on NanoString platform using microgram-level FNA samples and excellent portability to frozen tissues and RNA-Seq transcriptomic data. This workflow shows great potential in revolutionizing research and clinical practice of cancer molecular diagnosis.