AUTHOR=Gao Xinya , Lai Yuanhui , Zhang Zhanqiang , Ma Yanfei , Luo Zhizhai , Li Yanghong , Yang Ciqiu , Lu Guanming , Li Jie 

TITLE=Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis

JOURNAL=Frontiers in Oncology

VOLUME=Volume 10 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.00948

DOI=10.3389/fonc.2020.00948

ISSN=2234-943X

ABSTRACT=Background: RP11-480I12.5 is a newly identified long noncoding RNA (lncRNA) that has never been studied in breast cancer. The biological function of RP11-480I12.5 in breast carcinoma and its underlying mechanism are still unknown.
Methods: We scanned the Cancer Genome Atlas (TCGA) database and identified RP11-480I12.5 as one of the most dysregulated lncRNAs. The level of RP11-480I12.5 was assessed in breast cancer (BC) tissue samples and BC cell lines. The prognostic value of RP11-480I12.5 expression was assessed using the Kaplan-Meier method. The biological influence of RP11-480I12.5 on BC cell lines was studied using proliferation, Transwell migration, and invasion assays.
Results: RP11-480I12.5 was upregulated in both the TCGA database and our own database. Moreover, Kaplan-Meier and Cox proportional hazards analyses indicated that high RP11-480I12.5 expression was related to poor overall survival. Moreover, RP11-480I12.5 promotes the proliferation, migration and invasion of BC. RP11-480I12.5 promotes the expression of AURKA and the downstream Wnt/β-catenin pathway by sponging miRNA miR-490-3p.
Conclusion: Taken together, our results indicate that RP11-480I12.5 is associated with tumour progression in breast cancers. Our findings indicate that the long noncoding RNA RP11-480I12.5 promotes the proliferation, migration and invasion of breast cancer cells through the miR-490-3p-AURKA-Wnt/β-catenin axis, which may serve as a therapeutic target in the future.