AUTHOR=Polaskova Kristyna , Merta Tomas , Martincekova Alexandra , Zapletalova Danica , Kyr Michal , Mazanek Pavel , Krenova Zdenka , Mudry Peter , Jezova Marta , Tuma Jiri , Skotakova Jarmila , Cervinkova Ivana , Valik Dalibor , Zdrazilova-Dubska Lenka , Noskova Hana , Pal Karol , Slaby Ondrej , Fabian Pavel , Kozakova Sarka , Neradil Jakub , Veselska Renata , Kanderova Veronika , Hrusak Ondrej , Freiberger Tomas , Klement Giannoula Lakka , Sterba Jaroslav 

TITLE=Comprehensive Molecular Profiling for Relapsed/Refractory Pediatric Burkitt Lymphomas—Retrospective Analysis of Three Real-Life Clinical Cases—Addressing Issues on Randomization and Customization at the Bedside

JOURNAL=Frontiers in Oncology

VOLUME=Volume 9 - 2019

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2019.01531

DOI=10.3389/fonc.2019.01531

ISSN=2234-943X

ABSTRACT=In order to identify reasons for treatment failures when using targeted therapies, we have analyzed comprehensive molecular profiles of three relapsed, poor-prognosis Burkitt lymphoma cases. All 3 cases had resembling clinical presentation and histology and all three patients relapsed, but their outcomes differed significantly. The samples of their tumor tissue were analyzed using whole exome sequencing, gene expression profiling, phospho-proteomic assays and single cell phospho-flow cytometry. These results explain different treatment responses of the three histologically identical but molecularly different tumors. Our findings support personalized approach for patient with high risk, refractory and rare diseases and may contribute to personalized and customized treatment efforts for patients with limited treatment options like relapsed/refractory Burkitt lymphoma.