Front. Nutr. Frontiers in Nutrition Front. Nutr. 2296-861X Frontiers Media S.A. 10.3389/fnut.2023.1223638 Nutrition Review Integrating the latest biological advances in the key steps of a food packaging life cycle Rzayeva Aynura12 Coffigniez Fanny1* Zeynalov Nizami2 Gontard Nathalie1 Guillard Valérie1 1IATE, Agro Polymers Engineering & Emerging Technology, Univ Montpellier, INRAE, Institut Agro, Montpellier & CIRAD, Montpellier, France 2Nanostructured Metal-Polymer Catalysts, Institute of Catalysis and Inorganic Chemistry, Ministry of Science and Education Republic of Azerbaijan, Baku, Azerbaijan

Edited by: Sneh Punia Bangar, Clemson University, United States

Reviewed by: Tian Ren, Shaanxi Normal University, China; Mojtaba Yousefi, Semnan University of Medical Sciences, Iran

 *Correspondence: Fanny Coffigniez, fanny.coffigniez@umontpellier.fr 27 07 2023 2023 10 1223638 16 05 2023 05 07 2023 Copyright © 2023 Rzayeva, Coffigniez, Zeynalov, Gontard and Guillard. 2023 Rzayeva, Coffigniez, Zeynalov, Gontard and Guillard

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This literature review provides a focus on the potential of integrating the latest scientific and technological advances in the biological field to improve the status of the key steps of a food packaging life cycle: production, usage, post-usage, and long-term fate. A case study of such multi-biological food packaging is demonstrated based on the use of PHAs (polyhydroxyalkanoates) polymer, a microbiologically produced polymer from non-food renewable resources, activated by the use of bioactive components to enhance its usage benefits by reducing food loss and waste, displaying potential for reusability, compostability as post-usage, and finally, being ultimately biodegradable in most common natural conditions to considerably reduce the negative impact that persistent plastics have on the environment. We discuss how designing safe and efficient multi “bio” food packaging implies finding a compromise between sometimes contradictory functional properties. For example, active antimicrobials help preserve food but can hamper the ultimate biodegradation rate of the polymer. This review presents such antagonisms as well as techniques (e.g., coatings, nanoencapsulation) and tools (e.g., release kinetic) that can help design optimized, safe, and efficient active food packaging.

 sustainability biodegradable active food packaging natural active components usage benefit post-usage fate section-at-acceptanceNutrition and Food Science Technology Introduction

Although packaging plays a crucial role in the food supply chain by maintaining the quality and safety of food for a certain period, environmental problems due to resource depletion and persistent plastic accumulation from food packaging are of increasing concern. Indeed, the world’s plastic production is expected to increase three-fold by 2060, and ⅓ of this production will be used for packaging development (1, 2). In the meantime, 90% of this plastic ends up in the environment (through littering, landfilling, mismanagement, etc.): 3% directly in the ocean and 87% on the terrestrial continent (in soil), where they are fragmented through physical abrasion into micro and nanoparticles that diffuse into all environmental compartments (air, soil, water, and living organisms), resulting in considerable environmental and biological damage (1, 3). In this context, the emergence of new materials allowing the overall packaging sustainability to be increased is paramount: it clearly means (1) minimizing overall resource use and process step impacts, (2) ensuring product preservation to reduce food loss and waste, (3) reducing the burden of post-usage plastic waste management (e.g., by being compostable or recyclable) and, (4) paying special attention to the long-term outcome that can lead to the accumulation of plastic in the environment in the final stage of the materials life chain (3–5). One solution to address these multiple issues is to focus on materials that provide a biological solution at each stage: a biological resource, bioprocess transformation, bioactivity to better preserve food, and finally biological digestibility in the post-usage stages (Figure 1).

The expected effect of substituting fossil-based plastic materials with BIO4 ones – in the life cycle of food packaging materials (BIO4 = biobased, bioprocessed, bioactive, and biodegradable).

Therefore, to correctly use biobased resources for packaging development (e.g., plant or microbial polymers), it is necessary to use materials that are compatible with biomass temporal availability and the regeneration time and to avoid competition with food and feed uses. Moreover, bioprocess transformation refers to the use of biotechnologies to synthesize the material (e.g., microbial cells) with the net advantage that the range of resources to be used is considerably enlarged. For instance, with biotechnologies, it is possible to use organic wastes and other residues (6, 7). Bioprocesses also appear to be good alternatives to conventional chemical synthesis, because they require less time and additives, which can result in health problems in living marine and terrestrial organisms (endocrine disruptors, etc.) (8–10). Bioactive packaging refers to the integration of active bio components in the polymer, such as natural antimicrobials or antioxidants which can increase the packaging usage benefit by preserving food from deterioration, which leads to food loss and waste (FLW) mitigation, as demonstrated by several authors (11–13). Finally, biodegradable packaging means that the material could be disintegrated and then assimilated by microorganisms and digested into small unitary molecules (H2O, CO2, CH4) to produce a new microbial biomass. Ultimate biodegradation is currently the only solution to reduce conventional plastic accumulation in the environment (3).

Although several articles have already highlighted the importance of biodegradable packaging [see among others (14)], or the relevance of active, and smart biodegradable packaging for the food sector (15), or the applicability of biobased packaging for long-shelf-life foods (16), no publications to date have addressed all the four ‘bio’ aspects in a holistic approach to the packaging sustainability. It should be noted that in the web of sciences with the keywords “biodegradable, bioactive packaging” AND “biodegradable, bioactive, biobased packaging” AND “biodegradable, antimicrobial packaging” AND “biodegradable, biobased, antimicrobial packaging,” we obtained 90, 5, 215, and 6 review articles, respectively (carried out in February 2023).

In this context, the aim of this paper is to discuss the relevance of developing active packaging materials combining the four “bio” aspects (biobased, bioprocessed, bioactive, and biodegradable) by highlighting their challenges and limitations. Examples will be provided from commercial products or polymers that are still in development (e.g., PHAs, a family of polymers that, promisingly, covers the four “bio” aspects). This review highlights the importance of a reasoned-integrated approach to develop bio-benign, biodegradable, efficient active food packaging starting from the food needs in terms of preservation without compromising the ultimate biodegradability of the material. The importance of the use of biobased, bioprocessed, and biodegradable material will be discussed first, with examples of different polymers more or less in accordance with these aspects. The importance of the strategy of bioactive molecule incorporation is then developed, with the presentation of cutting-edge ways for incorporating active ingredients into biopolymers. Finally, the post-usage fate of the bioactive, biodegradable packaging materials is discussed.

 The importance of using biobased, bioprocessed, and biodegradable materials for food packaging Definition of biobased, bioprocessed, and biodegradable materials Biobased

The fear of a near future where oil-based resources are lacking and the societal demand for more ‘green’ materials have led to the development of polymers derived from renewable biological resources (‘biobased’).

For this purpose, research efforts have long focused on transforming plant or other non-fossil biogenic feedstocks into materials identical (e.g., bio-PET, bio-PE) or close to those derived from petroleum (e.g., PLA). At the same time, efforts have been made regarding the development of biodegradable solutions to solve the issue of plastic persistence in the environment, leading to the emergence, in the early 1990s, of the ‘bioplastics’ market, which includes both biodegradable and biobased plastics, with unfortunately a high degree of confusion regarding the distinction between them (17, 18). “Biobased” means materials partially or fully produced from biomass resources (such as sugar cane, hydrolyzed maize, rice or potato starch, etc.), irrespective of the end-of-life fate of the material (17). As such, a biobased polymer may be not biodegradable, such as bio-polyethylene (bio-PE), which is not more biodegradable than its oil-based counterpart. Several publications have sought to clarify this terminology and denounce the variations coming from the overall misinterpretation of the ‘bio’ prefix (17–19).

 Bioprocessed

Two categories of biobased polymers can be considered (20): the first one consists of materials whereby the chemical structure of the biomass feedstock is not maintained. This is the case, for instance, when biomass is used to obtain soluble sugars, which are then fermented to produce monomers for the polymerization (e.g., lactic acid to produce poly(lactic acid) or ethylene to produce biobased polyethylene). This is the route followed to obtain polymers that are identical to those derived from petroleum (e.g., bio-PET, bio-PE). The second category corresponds to a group of polymers directly synthetized by living organisms (plants, algae, microorganisms, etc.) for which the initial chemical structure is preserved after extraction and purification and then used as such [e.g., polyhydroxyalkanoates (21)], polymalic acid (22) or only slightly modified [e.g., celluloses acetates (23)].

To manufacture compounds belonging to the first category, significant efforts must be placed on the plant transformation, with selective chemical processes required to remove chemical functionality afforded naturally by many biobased feedstocks (e.g., many natural molecules contain oxygen or nitrogen, while this is not the case for fossil-based hydrocarbons). Agricultural crops produce precursors: mostly starch and soluble sugars, that can be directly used to obtain biobased polymers, or building blocks derived from selective transformation used to extract, fractionate, and deconstruct plant cell-wall polymers (cellulose, hemicellulose, pectin, lignin, etc.) (24). Additional chemical transformations are often needed to obtain the necessary functionality for polymerization of the biobased building blocks into polymers.

Beyond the economic and environmental cost of all this necessary chemistry, the use of dedicated agricultural crops for producing biobased polymers is highly controversial in a period of fears for worldwide food security (25). Most lifecycle analyses (LCA) show that biobased polymers are better than their oil-based equivalents in aspects such as GHG emissions and fossil fuel consumption but not for other indicators such as eutrophication, land occupation, etc., which leaves the question of environmental superiority of biobased polymers for further debate (26, 27).

In light of the questionable economic viability and environmental issues of biobased polymers chemically synthesized from biobased building blocks, a reasonable guideline would be to focus on the second category of polymers, those directly synthetized by living organisms and used after extraction/purification with minimal chemical transformations. To not compete with the food chain, they should be produced from agricultural or food by-products or residues. The use of residues and even organic wastes as feedstock to produce polymers is gaining more and more attention, especially in line with the development of circular economy schemes (7). They provide significant environmental benefit, provided that their collection, transport, and transformation are regionally-reasoned and do not necessitate expensive pre-treatments and transportation (28, 29).

 Biodegradable

Biodegradability is the intrinsic property of a material to be fully degraded by living microorganisms (e.g., soil microorganisms) into a new biomass and small non-toxic molecules such as water, carbon dioxide (CO2), and/or methane (CH4) in widespread natural environmental conditions and in a reasonable timeframe compatible with human life cycles (17). The term biodegradable is often and misleadingly applied to plastics that are biobased but not necessarily biodegradable, or that undergo degradation (e.g., lowering of the molar masses of macromolecules that form the material) but without complete, ultimate mineralization (30). The biodegradability of a polymer depends only on its chemical structure and not the carbon source. Polymers that are compostable are not necessarily biodegradable under ambient environmental conditions. For instance, PLA needs to reach a minimal temperature of approximately 60°C to be biodegraded, which can be achieved in industrial composting plants but not in soil for instance or even domestic compost (19). The risk of considering a polymer that turns out not to be biodegradable under ambient environmental conditions is the production of persistent micro and nano plastics with long-term adverse effects for the entire biosphere (3).

In light of the above, nonfood, nonfeed biobased, and minimally bioprocessed polymers appear to be the best compromise of biobased and bioprocessed polymers. In addition, care must be taken regarding the type of biodegradation that the material can achieve: ultimate biodegradation, e.g., complete degradation into mineral molecules, under common ambient environmental conditions, and in a reasonable timeframe are paramount.

 The various biobased and/or bioprocessed and/or biodegradable biopolymers Overview of the various types of biopolymers

Table 1 summarizes the various types of biopolymers already marketed and commercially available and also those that are still in development. Clearly, currently marketed biopolymers are not fully “bio” from their resources nor their end-of-life, or present limited usage benefit. They are either derived from food resources (e.g., PLA, PBSA, starch-based blends, or commercial PHBV) or not fully biodegradable under natural conditions (e.g., PLA), or not water resistant (e.g., starch-based blends).

The various biopolymers in development or available on the market.1

Biopolymer Global annual production in kilotons/year (2022 data)1 TRL achieved Resources: biobased (origin of material) Usage benefit: advantages/disadvantages Post usage fate
Bio-sourced, non-biodegradable, and non-compostable biopolymers ➔ 1-bio
Bio-PET 93 −⑨− Commonly 100% of biobased ethylene glycol (EG) and petroleum-derived terephthalic acid (TA); 100% biobased EG, and PTA production is feasible Very good processability, versatility, O2/CO2/H20 barrier Recyclable, non-biodegradable
Bio-PA 246 −⑨− 100% (vegetable biomass) Very good processability, versatility, O2/CO2 barrier Recyclable, non-biodegradable
Bio-PE 329 −⑨− 100% (e.g., sugar cane, sugar beet) Very good processability, extensibility, versatility, H20 barrier Recyclable, non-biodegradable
Oil-based, biodegradable, and home-compostable biopolymers ➔ 1-bio
PBAT 100 −⑨− Fossil Properties similar to LDPE, flexible (strain at break ∼710%) and tough, PBAT is used primarily in combination with another polymer Home compostable
PBSA 20 −⑨− 20–100% (corn, cane sugar, cassava, beets) Sensitivity to humidity, temperature variations, and UV Home compostable
Bio-sourced and compostable (industrial conditions) bio-polymers, conventional chemistry for polymerization ➔ 2-bio
PLA 460 −⑨− 100% (corn, cane sugar, cassava, beets) Low- and high-temperature behavior to be improved Industrially compostableEN 13432 or NFT 51–800
Biobased, biodegradable (in natural conditions), home-compostable, biosynthesized biopolymers ➔ 3-bio
Cellulose 273.31 million2 −⑨− > 90% (cellulose pulp) Tear resistance at low temperatures, lack of barriers (oxygen, fat, etc.) Home compostable
Starch-based materials 397 −⑨− 30–100% (maize, wheat, potato) Competition w/food Sensitivity to temperature variations, not water resistant Industrially compostableEN 13432 or NFT 51–800 & Home compostable
PHB/coHV (from noble food resources) 50 −⑨− 100% (corn, sugars, beet) Barrier properties similar to that of PP, brittle if low HV Home compostable
PHB/coHV (from organic wastes and residues) Still pilot-scale production −⑥− 100% (agricultural and food residues and wastes, urban wastes) Barrier properties similar to that of PP, brittle if low HV Home compostable
PHBH Undisclosed −⑨− 100% (pure oils, currently) n/a Home compostable
mcl-PHA3 Still experimental production −③− 100% (pure food resources, currently) n/a Home compostable

1http://www.european-bioplastics.org/bioplastics/materials/. 2Global annual production: Paper and cardboard for packaging: « La demande mondiale des matériaux des emballages papier devrait atteindre 273,31 millions de tonnes en 2022 contre 195,72 millions en 2014 » (https://www.graphiline.com/article/21772/previsions-marche-mondial-emballages-papier). 3Medium-chain-length PHA.

Only cellulose-based materials and PHAs obtained from non-food resources (such as PHBV) appear to fulfill all the criteria.

 PHAs, an example of biobased, bioprocessed, and biodegradable polymers

PHAs comprise a family of polymers with very versatile properties (water-insoluble, hydrophobic, thermoplastic). They can be molded into rigid or semi-rigid items using thermomechanical processes, and in their final shape, they exhibit good oxygen barrier properties that make them promising for fresh and other processed food packaging applications (31).

Commercial PHAs are currently nearly exclusively the copolymer polyhydroxy(butyrate-co-valerate), P(HB-co-HV). P(HB-co-HV) is a copolymer, meaning that it is a combination of two monomers, namely hydroxy-butyrate and hydroxy-valerate. P(HB-co-HV) is currently produced using pure microbial cultures fed with high-purity substrates that require (1) sterility (2) high energy, and (3) pure glucose or corn steep liquor as feedstock (48% of the total production cost). This contributes to a prohibitive market price (5 €/kg) to be used as a commodity polymer.

To address this issue, and to create a virtuous cycle rather than depleting one by using organic residues instead of apure carbon source, researchers have developed bioconversion processes of agri-food residues using optimized eco-efficient mixed microbial cultures (MMC). These processes decrease the investments and operating costs of the P(HB-co-HV) conversion with respect to pure culture and make it easier to use cheaper by-products such as feedstock (6, 32, 33). However, these processes are not yet available at an industrial scale.

Once produced, PHA macromolecules must be extracted and purified to remove all impurities (e.g., proteins, inorganic compounds) that can hamper its further use as a thermo-mechanical processable polymer. To do this, after harvesting cells from the bioreactor, flocculation, centrifugation, or filtration is used to remove the aqueous phase from the sedimented biomass. After a thermal drying or lyophilization step, the PHA-rich biomass is ready for extraction and purification (34, 35). Most people utilize methods on a laboratory scale such as solvent-based extraction (using halogenated solvents or halogen-free solvents such as butanol, or acetone, etc.), enzymatic (Alcalase®, lysozyme) or mechanical techniques (ultrasonication, high-pressure homogenization, osmotic pressure), or combinations of these methods. These methods result in cell disruption, enabling polymer solubilization in the solvent used. The last steps are then washing and drying the polymer to obtain a powder that can be stored and subsequently used (36).

PHA granules are often damaged by random and chain-end scission during solvent-based extraction processes. The final PHA purity and quality (e.g., molecular weight and polydispersity index) are thus affected by the extraction method. The solvent extraction method (especially halogenated solvents) provides the highest recovery with the highest purity and molecular weight (up to 98% and 0.8 MDa, respectively) (33, 35–38). However, because of the cost and the environmental impact of solvents, their use remains possible only at the laboratory scale, for research purposes. Beyond extraction, purification is also very important and has been found to greatly impact the processability of the polymer and its final properties (39); it was observed that multiple purification steps lead to better P(HB-co-HV) mechanical properties after processing. Because considerable quantities of hazardous solvents and energy are necessary during the extraction and purification steps, PHA production is not fully bio-benign. Moreover, the halogenated solvents used for the extraction can cause severe health issues in living organisms as well as environmental problems (40).

Above all, PHAs are biodegradable under various composting conditions (including home-compositing) and other natural environments (e.g., soil), making them particularly suitable to fight pollution by persistent plastics. As a result of successful tests on the biodegradation of PHA in activated sludge, compost, aqueous environments, and soil, the biodegradation of PHA blends with natural fillers in composting conditions is faster than with pure PHAs (41–43). This high biodegradation rate is the result of the involvement of a wide range of microorganisms and fungi, similar to the ones used for PHA biosynthesis, that can biodegrade PHAs in anaerobic and aerobic conditions (37, 44–48). Environmental factors such as temperature, pH, moisture, oxygen concentration, sunlight, the number/amount of microorganisms, as well as the chemical structure of PHA such as the percentage of 3 HV, are known to affect the rate of biodegradation of these polymers (49).

In summary, PHAs can be synthesized from agri-food wastes and residues using bioprocessed (microbial synthesis) and they are biodegradable under natural conditions and ambient temperature. They are thus promising candidates to turn the current linear plastic chain into a more sustainable cycle. Moreover, PHAs exhibit good usage benefits, with high gas/water barrier properties as well as good crystallization and mechanical properties; making them good candidates to prolong the shelf-life of food products. PHAs can also be converted into bioactive materials to increase their packaging usage benefit of reducing food losses.

 The importance of bioactive functionality in food packaging Bioactive packaging to better preserve food from deterioration

Commission Regulation [EC 450/2009 (50)] defined Active Packaging (AP) materials as follows “Materials and articles that are intended to extend the shelf-life, or to maintain or improve the condition, of packaged food; they are designed to deliberately incorporate components that would release or absorb substances into or from the packaged food or the environment surrounding the food.” As described in Table 2, various scavenging and releasing systems have been developed, aimed at decreasing the deterioration processes in food, such as microorganism growth, oxidation, etc. (61).

The various scavenger and releasing systems developed for food applications (51, 52).

Type of system Absorbed or released molecules Potential uses of the active packaging Shelf-life gain examples References
Scavenger Oxygen Prevent aerobic microbial and mould spoilage, oxidation, rancidity, color change, vitamin loss 3–9 days (bakery products) (53)
Carbon dioxide Absorb the excess CO2 produced by fermented and respired products Up to 14 days (strawberries) (54)
Moisture Prevent microbial and mold growth, maintain low humidity for dry and respired products Up to 60 days (Portuguese cheese) (55)
Ethylene Reduce ripeness and senescence for F&V Up to 14 days (bananas) (56)
Releasing Carbon dioxide Prevent microbial and mold growth 2 days (cod fillets) (57)
Ethanol 16 days (bread) (58)
Antimicrobial compounds Up to 14–15 days (beef meat) (59)
Antioxidant compounds Prevent lipid and vitamin oxidation 4 weeks (corn oil) (60)

For example (62), showed that 0.5% of gallic acid in PHBV trays of meat, fish, or cheese packaging can induce the absorption of 7.7% of oxygen in 10 days (derived from food desorption or oxygen entrance through the packaging), thus limiting oxidation and microbial aerobic growth. Similarly, several authors have shown that the inclusion of different essential oils such as buriti oil (1.5 wt.%), orange leaf essential oil (2 wt.%), or thymol (6 or 8 wt.%) in chitosan, gelatin, or PLA films exhibited antimicrobial activity against E. coli, P. aeruginosa, S. aureus, and B. subtilis, as well as C. albicans and A. niger, making them suitable strategies to increase food shelf-lives in different sectors (63–66). Going beyond these studies demonstrating antimicrobial effects on only a single microbial species Alparslan et al. (67) tried to quantify the shelf-life gain obtained on fresh shrimp packed in gelatin film containing 2% orange leaf essential oil: the shelf-life increased from 8 days with gelatin film to 14 days with the active film. With the same objective of benefit quantification Suwanamornlert et al. (66) reported that the shelf-life of bread increased from 6 days (control with PLA packaging) to 9 days with PLA film containing 6 wt.% thymol.

It should be noted that essential oils extracted from natural aromatic plants (e.g., oregano, thyme, eucalyptus, rosemary, clove, cinnamon etc.) is often used as natural antioxidant, antimicrobial, and antifungal compounds for AP (68–70). Indeed, consumer demand for food products without synthetic chemical preservatives has promoted EO usage in the food packaging industry in recent years (68). As for all AP, the use of active components directly in the packaging material instead of in the food allow reduction of the use of preservatives, as the active component is slowly released toward the surface where it is needed (on the food surface).

To obtain safe and efficient active materials, the quantity of active compounds added in the formulation of active systems is defined by the expected efficiency but also regulatory needs and sensory aspects (e.g., active compounds that are also flavoring agents). Indeed, a specific admissible daily intake (ADI = the quantity of this specific compound that can be eaten without a negative impact on the health) is defined by regulations for all potential active substances (71). The use of the active molecules is possible only if the natural exposure of consumers to those substances from other (natural) sources (natural occurrence in food products due to their composition) does not exceed the ADI (Figure 2). The natural exposure depends on the country, food consumption habits, and the consumer’s age. This knowledge allows determination of the maximal quantity of the AC that can be used for active packaging (ADI minus the natural exposure) (Figure 2).

Principle of antimicrobial compound dimensioning.

The choice of the best molecule to develop an active antimicrobial packaging also depends on the capacity of the molecules to act on the targeting of specific microorganisms. Consequently, the appropriate AC can differ from one product to another, depending on the microorganisms prone to growth on the product. To correctly design the active packaging, it is necessary to confirm that the quantity that can be used for the active packaging, while remaining below the difference between the ADI and natural exposure, is enough to reach the minimal inhibitory concentration (MIC) of the targeted microorganisms [EC 450/2009 (50)] (Figure 2). The use of modeling tools, taking into account the release and migration (for volatile molecules) or diffusion characteristics (for non-volatile molecules) of the AC in defined storage conditions (temperature, duration) of the product, as well as MIC for the targeted microorganisms allows precise determination of the quantities of antimicrobial agents to use in packaging to preserve the food product from deterioration (13, 72). For some molecules with high odor or taste, such as essential oils, the quantity of AC to use should be below the odor threshold and avoid organoleptic modifications (Figure 2), as stipulated in the European regulations [1935/2004 EC (73)] and [EC 450/2009 (50)].

 Strategies for active functionality incorporation

The impact of antimicrobial compounds, such as essential oil, on microorganism inhibition in food is dependent on the release and migration capacity of the molecules, and as a consequence on (1) the strategy of AC incorporation in the polymer material, (2) the volatility of the AC, and (3) the interactions and bonds between the component and the polymer material (74, 75). Indeed, different authors have shown that a film containing a homogenous concentration of AC throughout generated a slower and more sustained release than a film with an active coating concentrating the entire quantity of AC on the film surface (Figure 3) (76–78). This structure of AP and strategy of AC incorporation impact the preservative action of the AC on the food (Figure 4). For example, Wicochea-Rodríguez et al. (79) showed a four-fold lower release constant for carvacrol included in soy protein isolate film compared with the equivalent solution (carvacrol in soy protein isolate) coated on paper. Moreover, the authors also showed that the production of the same films with eugenol instead of carvacrol considerably increased the release of the molecule (release rate constant 100 times higher) due to the higher volatility of the molecule and lower retention by polymer, thereby inducing a different mechanism of release.

Hypothetical dependence of the active functionality incorporation methods, meaning (1) in coated and (2) “Throughout” material on the migration of AC and biodegradation of the film.

The various film structures and active volatile compound incorporation strategy (Active Compound Throughout and Active Compound in a surface coating) in packaging.

The choice to integrate AC throughout versus on the surface of polymers can also generate different degrees of loss of the molecules, depending on the production processing. Indeed, integration of AC directly in the polymer usually implies that the AC is subjected to thermal processes, such as extrusion or compression molding, meaning that highly sensitive molecules can be degraded, thus reducing their antimicrobial activity. By contrast, integration of AC on the surface of the polymer usually implies no thermal coating process, such as a bar, knife, spray coaters, or electrospinning, which preserves AC and its antimicrobial activity. The main principle of both of these physical incorporation methods is that the active components are physically contained or dispersed within the packaging material. These methods do not involve any chemical reactions or modifications of the active components, unlike sol–gel processes, surface immobilization, cross-linking, and covalent binding methods that involve chemical reactions (covalent and non-covalent bonds) between antimicrobial molecules and the film surface (77, 78, 80). The advantages and disadvantages of these incorporation methods are listed in Table 3.

Advantages and disadvantages of physical and chemical active functionality incorporation methods.

Methods Definition Advantages Disadvantages References
Physical
Extrusion Continuous production of material by melting polymer and pushing it through the rotating screw Low cost, high-volume production, suitable for different types of polymers, obtaining of a homogenous material AC can be degraded due to the applied temperature in the process (77)
Compression molding Production of the film by applying pressure on pre-positioned polymer in the mold between hot plates
Solution casting Casting a polymer-dissolved solution on support where it solidifies thanks to the volatility of the solvent Non-thermal process suitable for throughout formulations and coating Use of solvents potentially toxic, difficulty to obtain a homogeneous material (77, 81)
Knife coating Spreading coating material on polymer support with a knife spreader Simple, scalable, low cost Use of solvents potentially toxic, flat surface requirement, difficulty to obtain a homogeneous material (77)
Spray coating Deposition of atomized droplets fluid as a coating on a polymer support
Bar coating Spreading coating material on a polymer support with a Mayer bar spreader Simple, low cost Limited scalability, use of solvents potentially toxic, flat surface requirement, difficulty to obtain an homogeneous material
Dip coating Immersion of the film substrate into a bath containing coating material followed by dwelling, withdrawal, and drying steps Limited thickness control, use of solvents potentially toxic, difficulty to obtain a homogeneous material
Layer by layer Deposition of alternating layers of oppositely charged materials with wash steps in between Low cost, suitable for large surface areas Limited thickness control, a long processing time (77, 82)
Spin coating Application of coating materials on a flat support by using centrifugal force Low cost, thin and uniform coating, quick drying Not scalable, use of solvents potentially toxic, flat surface requirement (77)
Electrospinning/spraying Production of continuous nanoscale fibers/particles from polymer solution with the aid of a high-voltage electric field by pumping through a spinneret Low cost, scalable, suitable for different types of polymers and AC Use of solvents potentially toxic, difficulties in processing due to various impacts (C (83).)
Chemical
Sol–gel process Formation of a gelatinous three-dimensional network (gel) from inorganic colloidal suspension (sol) (solution containing – antimicrobial and precursor of the inorganic phase) through hydrolysis and condensation High-quality materials with homogeneity and purity Long processing time (77, 84)
Surface immobilization Immobilization of AC on the film surface with materials that have compatible functional groups (proteins, enzymes, peptides, etc.) Better stability and control of the AC release rate Additional action could be needed to immobilize AC on the film surface (spacer, crosslinker); antimicrobial activity may be reduced due to the formed strong bonds. (77)
Cross-linking Formation of covalent bonds or ionic bonds to link the AC (with crosslinkers) to the polymer Improvement of the thermal and mechanical stability of the material, modification of the release rate, and formation of a three-dimensional polymer network structure Reduction of the bioactivity, increase the stiffness of the material, high cost (85)

To overcome the issue of thermal degradation during thermomechanical shaping processes, attention has been given in the past two decades to different encapsulation strategies. Beyond mitigation and protection of AC from thermal degradation during packaging production, encapsulation may also help to control the release of the active molecules, especially if they are volatiles, and it is also used for that purpose (86). The term “encapsulation” was defined by Becerril et al. (87) and Stoleru and Brebu (88) as the technique that permits a substance (the active agent) to be encapsulated within another, resulting in tiny particles, the contents of which are gradually released under certain conditions. Hence, encapsulation strategies permit savings in regard to some active components as it is no longer necessary to compensate for the AC thermal degradation by spiking the initial load of AC in the material. In the same way, it allows minimization of the negative impact of the EOs on odors and organoleptic characteristics of the food products (89–91). Finally, encapsulation allows AC losses during storage (e.g., AC trapped into a polymer capsule) to be avoided and to trigger the release only when it is necessary, e.g., in food contact by action of humidity for instance as is the case for cyclodextrin-based capsules (92). Various carriers have been tested to develop encapsulation strategies, such as cyclodextrins, (nano)clays, nanofibers, and nanoparticles, all of which exhibit some advantages and disadvantages, as listed in Table 4 (87, 94).

Advantages and disadvantages of the various encapsulation strategies used for active packaging.

Type of carriers Definition Advantages Disadvantages References
Cyclodextrins The AC is trapped in cyclic oligosaccharides with a truncated cone shape, including an inner hydrophobic core and outer hydrophilic shell. ➔Highly effective control of the AC release, CDs are opened with high humidity➔Suitable to encapsulate hydrophobic ACs➔Stabilization of the volatile compounds ➔High price (76, 92–94)
Nanoclays The AC is trapped in a nanoparticle of mineral silicates (diameter size < 100 nm) with varying chemical compositions and morphologies (e.g., halloysite, montmorillonite, etc.). ➔Natural➔Low price➔Continuous release of the AC➔Capacity to absorb volatile substances, such as off-flavors and off-odors ➔Migration capacity of clays: problematic for consumer safety (95–97)
Nanofibers The AC is trapped in fibers (1 nm < diameter size < 1 μm) produced by electrospinning techniques (use of an electric field on polymer solutions) and can be coated on a substrate or interlayers of biopolymer films. ➔Effective for trapping and release of the AC➔High loading capacity➔Low price ➔Difficult process because dependent on polymer and solvent characteristics, as well as processing parameters and environmental conditions, to obtain fibers with relevant morphologies (size, shape) (83, 98, 99)
Biopolymeric carriers: nanospheres, nanocapsules The AC is directly trapped inside a nanocarrier core encased in a thin polymer layer. ➔Biodegradable➔Non-toxic➔Low price➔Controlled release of AC➔Improved functional properties of the AP➔Stabilization of the volatile compounds ➔Utilization of toxic solutions in production➔Well suited to lab scale, but difficult to upscale (100, 101)
How to reach the right balance of antimicrobial properties during the usage stage and biodegradation in the post-usage stage

To design bioactive and biodegradable packaging, it is crucial to consider the impact of natural antimicrobial components in polymers on the degradation capabilities of the entire packaging. Thus, it is necessary to achieve the right balance between the antimicrobial characteristics of packaging materials to increase the food shelf-life during the usage phase and the biodegradation of packaging during the post-usage phase. The influences of the incorporated bioactive components on the biodegradation properties of active biobased materials are reviewed in Table 5.

Effects of bioactive components on the antimicrobial/antioxidant activity and biodegradation of biopolymers.

Polymer/Composite Production method Formulation strategy Bioactive component Antimicrobial/Antioxidant effect Biodegradation condition Biodegradation rate AA or MP effect Reference
Delayed and/or slowed down
Gliadin Casting Throughout Cinnamaldehyde - Compost Biodegradation rate decreased as the % of cinnamaldehyde increased MP (102)
Starch-PVA Casting Throughout Neem EO, Oregano EO - Compost EOs slightly decreased the biodegradation rate, especially Oregano EO MP (103)
Chitosan Casting Throughout Buriti oil S. aureus, E. coli, P. aeruginosa Soil Biodegradation rate decreased with increasing Buriti oil content, explained by increasing hydrophobicity with EO MP (63)
Starch Casting Throughout Rosemary extract Antioxidant activity Compost Biodegradation rate decreased by the presence of RE (time lag) AA (104)
Starch/Cassava/Sugarcane Compression molding Throughout Oregano EO S. aureus, E. coli Soil Biodegradation rate decreased with increasing Oregano EO %, explained by increasing hydrophobicity with EO AA (105)
Starch/PCL/Starch Compression molding/Electrospinning Throughout/Multilayer film Carvacrol E. coli Compost Slower biodegradation rate AA (106, 107)
PHBV/PLA-PHB Electrospinning Throughout/Bilayer film Catechin Strong antioxidant activity Compost Slightly slower disintegration rate AA (108)
Cassava/CMC Casting Coating Turmeric A. niger Compost 15 μL TEO slowed the disintegration rate (64)
PHB/PHA Casting Throughout Grape seed lignin Antioxidant activity Compost High % of lignin slowed the biodegradation rate AA (109)
Chitosan/Pigskin gelatin and Chitosan Casting Throughout Boldo-do-Chile extract Psychrotrophic microorganisms, antioxidant activity Compost Disintegration time reduced MP (110–112)
PHB Casting Throughout Eugenol Salmonella sp., S. aureus, E. coli and A. niger Agricultural, sandy, and landfill soil Biodegradation in sandy soil was slower than in other soil types (113)
Pectin Casting Nanoemulsions/Throughout Copaiba S. aureus, E. coli Soil Slower biodegradation rate AA (114)
Chitosan Casting Throughout Quercus extract B. subtilis, antioxidant activity Industrial compost, vineyard soil, and garden soil QE reduced the biodegradation rate in all soil types AA (115)
Gelatin Casting Throughout Citrus lignocellulosic fibers B. subtilis, S. aureus, E. coli, P. aeruginosa, C. albicans, strong antioxidant Soil Increasing ratios of fibers decreased the biodegradation rate MP (116)
Chitosan/Cin/Chitosan Casting Coating/Multilayer film Cinnamon S. aureus, E. coli Soil Biodegradation rate of the films with 0.5% Cinnamon EO reduced MP (117)
PLA/PCL Casting Throughout Green tea extract Antioxidant activity Compost GTE with 30 wt.% decreased the biodegradation MP (118)
Accelerated
PLA-PHB Compression molding Throughout D-Limonene - Compost D-limonene accelerated the disintegration of the PLA-PHB-D-limonene blends MP (119)
PLA/Ag Extrusion, injection molding Throughout Thymol - Compost Thymol increased the biodegradation rate MP (120)
PLA/Cellulose Nanocrystals Extrusion Throughout Lignin Xhanthomonas axonopodis pv. vesicatoria and Xhantomonas arboricola pv. pruni Compost Increased biodegradation rate of the composites with 3 wt.% lignin (121)
Starch Casting Throughout Yerba mate extract - Compost YME increased the biodegradation rate MP (122)
Bean starch Casting Throughout Cocoa nibs extract Strong antioxidant Compost CNE increased the biodegradation rate MP (123)
PCL Extrusion Throughout Grape seed extract L. monocytogenes Soil GSE increased the biodegradation rate MP (124)
PLA/Organoclay Extrusion Supercritical impregnation of the ACs in PLA/Organoclay Thymol, Cinnamaldehyde S. aureus, E. coli Compost Both thymol and cinnamaldehyde increased the biodegradation rate MP (125)
PLA Casting Throughout Cocoa bean shells Strong antioxidant Water CBS presence increased the biodegradation rate MP (126)
PLA Casting Throughout Propolis E. coli, antioxidant activity Soil Propolis addition increased the biodegradation rate (127)
PLA/Nanoclay Compression molding Throughout/Nanoclay Thymol S. aureus 8,325–4, E. coli Compost Nanoclay increased the biodegradation rate MP (65)
PLA/Nanofibrillated cellulose Casting Throughout/Nanoclay Thymol, Curry Fungal growth Soil Thymol and NFC increased the biodegradation rate MP (128)
Non affected
Starch Casting Nanoemulsions/Throughout Lemongrass EO S. aureus, E. coli Compost LEO addition did not affect the biodegradation - (129)
Bacterial cellulose Casting Throughout Lauric acid B. subtilis Compost Biodegradation was not affected by LA addition - (130)
PLA/CNC/Chitosan Extrusion, Electrospinning, Coating Throughout/Multilayer film Ethyl lauroyl arginate L. innocua, S. enterica Compost LAE presence did not reduce the biodegradation rate - (131)

AA, antimicrobial activity; MP, modified properties.

All studies (Table 5) showed that the impact of active components on polymer biodegradation was due to either (1) the antimicrobial activities of the AC or (2) modification of polymer properties owing to the presence of AC (132, 133).

In the first case, essential oils and natural bioactive components have a high probability to hamper the biodegradation of biopolymers, as they are per se antimicrobial compounds and poorly biodegradable due to the presence of quaternary carbon atoms and fused or bridged ring systems (134). Most of these AC are extracted from thyme, mint, sage, parsley seed, and spearmint oil, and they are toxic to microorganisms, including those involved in biodegradation (135, 136). Various effects can be expected: a delay in the biodegradation kinetics (time lag) without further impact on the biodegradation rate, a slowing down of the biodegradation rate, or a decrease of the maximal biodegradation level, as shown in Figure 5. A combination of these three different impacts may also occur. An impact on the disintegration (the first step of the biodegradation process before assimilation by the microorganisms) has also been reported (108).

Different biodegradation scenarios for active biopolymers.

For instance, Tampau et al. (107), showed that biodegradation of a starch/PCL/starch multilayer containing carvacrol (15 wt.% PCL) in compost remained incomplete, with a maximum of 85%, after 45 days compared to the starch/PCL/starch film without carvacrol that is 100% biodegraded in the same conditions and duration, while Piñeros-Hernandez et al. (104) showed that biodegradation of starch film containing between 5 and 20% rosemary extract (polyphenol) was retarded in compost conditions (Table 5) but that the final biodegradation level was not affected.

However, although a decrease in biodegradation because of antimicrobial activity of AC was often observed, the majority of studies cover only active packaging with a non-encapsulation AC throughout the entity (Table 5). Consequently, the impact of the incorporation strategies, i.e., encapsulation or not, coating vs. incorporation throughout, etc. is very absent from the literature. Indeed, the presence of the AC on the surface of the packaging instead of throughout the entity increases the release of the molecules during the usage stage (see Section 3.2). Therefore, biodegradation should be impacted less, as the concentration of AC is lower and the polymer is more available for microorganisms at the post-usage stage (Figure 3). In this vein, Mustapha et al. (64), showed that a decrease in the coating thickness of starch with turmeric oil induced an increase in biodegradation. Consequently, a high level of importance should be placed on the design of active packaging to find the best formulation allowing the shelf-life to be increased without delaying the biodegradation of the material. For this, mathematical modeling could be developed, coupling the alteration of the microorganism’s growth in food and their impact on the product shelf-life, as well as the growth of the biodegradation microorganism and the impact on the end-of-life of the material.

In the second case, the addition of AC in polymers can either reduce or increase the polymer biodegradation due to modification of their properties. A decrease in biodegradation can be due to an increase in the molecular weight or hydrophobicity, or a decrease in flexibility, as has been observed with the presence of cinnamaldehyde (1.5 wt.% to 5 wt.%) in gliadin film during compost (102) or cinnamon (0.5 wt.%) in chitosan/starch film during biodegradation in soil (117). Indeed, EOs formed bonds with the polymer chains, and this cross-linking increased the glass transition temperature. It also restricted the entrance of water into the polymer matrix, which led to less swelling and lower water vapor diffusion coefficients. By contrast, the increase in biodegradation can be due to an increase in hydrophilicity, thermal stability, and chain mobility and a decrease in the molecular weight and crystallinity, as has been observed with the presence of green tea extract (from 10 to 30%) in PCL/PLA film in compost (118), or olive pomace (15%) in PHBV film during biodegradation in soil (137). It should be noted that AC encapsulated in nano clays, such as thymol in PLA-based nano-composites, cinnamaldehyde in PLA/organoclay, or curry/thymol in nanofibrillated cellulose/PLA, led to an increase in the film’s biodegradation owing to higher sensitivity to hydrolysis of the polymeric chains, which highlights the relevance of encapsulation of the AC (65, 125, 128). However, the impact of AC on the biodegradation of the polymer can differ due to the amount of the AC and reach a breaking point with an increase/or decrease of the AC quantity, whereby polymer biodegradation can decrease because of significant deterioration of the polymer properties. For example, the presence of 20 wt.% green tea extract in PCL/PLA accelerated the biodegradation of the entire material owing to increased hydrophilicity, but 30 wt.% green tea extract in PCL/PLA decreased the biodegradation of the entire material owing to an increase in crystallinity (118). By contrast, the integration of 3 wt.% lignin into a cellulose nanocrystals/PLA system resulted in acceleration of the disintegration of the film, but the presence of only 1 wt.% lignin in the system resulted in slower biodegradation due to a higher degree of crystallization of the material (121).

Finally, in some rare cases, the presence of AC in the polymer did not impact the biodegradation of the entire material, as observed with lemongrass EO and lauric acid in starch (129, 130), and ethyl lauroyl arginate (LAE) in a PLA/PLA-LAE/PLA-CNC/Chi structure (131).

Beyond AC incorporation that results in modification of the polymer properties, conditions met during biodegradation also affect the biodegradation rate (humidity, nature of the soil, etc.). Therefore, the type of active molecules, their incorporation strategy, their interaction with polymer, and on polymer properties, are among the many parameters that can affect (positively or negatively) the biodegradation of the bioactive material and should hence be taken into account. Beyond these observations, the comprehension of the interactions of these different parameters still needs to be deepened to better design active packaging, allowing for an increase in food shelf-life by maintaining or even enhancing the biodegradation of the packaging.

 Conclusion

The continuous increase in plastic accumulation and production worldwide, and the very substantial environmental impact of food loss and waste, are the main reasons for improving the overall sustainability of food packaging materials. Therefore, an appropriate choice of material is paramount to tailoring sustainable food packaging. The use of materials derived from biobased sources, which do not compete with food and feed resources, has resulted in bioprocessing for environmentally friendly production. They are fully biodegradable by ensuring their digestibility under natural conditions, as is the case for PHAs, and they are showing great promise to enhance sustainability. Furthermore, bioactive materials help preserve food by minimizing deterioration, such as microbial development or oxidation, consequently reducing food loss and waste and its negative environmental impact. However, to design efficient antimicrobial packaging, the AC has to be present in sufficient quantity to reach the minimal inhibitory concentration of target microorganisms without surpassing the admissible daily intake. To achieve this goal, various strategies for the incorporation of AC in film (coating or throughout the polymer) have been developed with different encapsulation options, thereby enabling control of the release of the AC. However, it is crucial to achieve a proper equilibrium between antimicrobial functions during usage and biodegradability in the post-usage phase. Yet, in the case of natural active components (EOs, plant extracts, etc.), it has been shown that the amount and type of added AC, its incorporation strategy into the polymers, and its impact on the polymer’s properties can either positively or negatively impact the biodegradation properties of the biopolymer. Consequently, in the future, better comprehension and prediction of the impact of AC on the shelf-life of products and biodegradation of the material should be considered as a whole, through the use of modeling tools, to better design sustainable packaging.

 Author contributions

AR and FC wrote the first draft of the manuscript. All authors contributed to the conceptualization, design of the present review, manuscript revision, read, and approved the submitted version.

 Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

AR acknowledges one-year Ph.D. salary funding from the Azerbaijan National Academy of Sciences (ANAS).

 References OECD. 2022. Policy Scenarios to: Global Plastics Outlook (2060). Europe Plastics. Plastics - the Facts 2021. (2021) Available at: https://plasticseurope.org/knowledge-hub/plastics-the-facts-2021/ Gontard NDavid GGuilbert ASohn J. Recognizing the long-term impacts of plastic particles for preventing distortion in decision-making. Nat Sustain. (2022) 5:472–8. doi: 10.1038/s41893-022-00863-2 Andrade M. S. Ishikawa O. H. Costa R. S. Seixas M. V. Rodrigues R. C. Moura E. A. Development of sustainable food packaging material based on biodegradable polymer reinforced with cellulose nanocrystals. Food Packag Shelf Life. (2022) 31:100807. doi: 10.1016/j.fpsl.2021.100807 Guillard VGaucel S. Fornaciari C. Angellier-Coussy HBuche PGontard N. The Next Generation of Sustainable Food Packaging to Preserve Our Environment in a Circular Economy Context. Front Nutr. (2018) 5:121. doi: 10.3389/fnut.2018.00121 Albuquerque M. G. Adiutori R Trindade N. M. Carmo I. T. D. Oliveira C. S. S. Pardelha F . Biobased feedstock valorisation through polyhydroxyalkanoate production: from excess cheese whey to eco-efficient bioplastics. Environ Eng Manag. J. (2012) 11. Available at: http://omicron.ch.tuiasi.ro/EEMJ/ Gontard N. Sonesson U. Birkved M. Majone M. Bolzonella D. Celli A. Sebok A. A research challenge vision regarding management of agricultural waste in a circular bio-based economy. Crit Rev Environ Sci Technol Adv. (2018) doi: 10.1080/10643389.2018.1471957 Hermabessiere L. Dehaut A. Paul-Pont ILacroix C. Jezequel RSoudant P. Occurrence and effects of plastic additives on marine environments and organisms: A review. Chemosphere. (2017) 182:781–93. doi: 10.1016/j.chemosphere.2017.05.096 Pires A. Cuccaro ASole MFreitas R. Micro(nano)plastics and plastic additives effects in marine annelids: A literature review. Environ. Res. (2022) 214:113642. doi: 10.1016/j.envres.2022.113642 Scott L. Environmental Risk of Polymers and their Degradation Products (PhD thesis). (2013) Catarino M. D. Alves-Silva J. M. Fernandes R. P. Gonçalves M. J. Salgueiro L. R. Henriques M. F. . Development and performance of whey protein active coatings with Origanum virens essential oils in the quality and shelf life improvement of processed meat products. Food Control. (2017) 80:273–80. doi: 10.1016/j.foodcont.2017.03.054 Correa J. P. Molina VSanchez M. Kainz C. Eisenberg PMassani MB. Improving ham shelf life with a polyhydroxybutyrate/polycaprolactone biodegradable film activated with nisin. Food Packag Shelf Life. (2017) 11:31–9. doi: 10.1016/j.fpsl.2016.11.004 Menezes N. M. C. Martins W. F. Longhi D. A. Aragão G. M. F.de. Modeling the effect of oregano essential oil on shelf-life extension of vacuum-packed cooked sliced ham. Meat Science. (2018) 139, 113–119. doi: 10.1016/j.meatsci.2018.01.017 Siracusa VRocculi P. Romani S. Rosa MD. Biodegradable polymers for food packaging: a review. Trends Food Sci Technol. (2008) 19:634–43. doi: 10.1016/j.tifs.2008.07.003 Amin U. Khan M. K. I. Maan A. A. Nazir A. Riaz S. Khan M. U. Lorenzo J. M. (2022). Biodegradable active, intelligent, and smart packaging materials for food applications. Food Packag Shelf Life. 33:100903. doi: 10.1016/j.fpsl.2022.100903 Peelman N. Ragaert P. Verguldt E. Devlieghere F. Meulenaer B.de (2016). Applicability of biobased packaging materials for long shelf-life food products. Packag Res. 1. doi: 10.1515/pacres-2016-0002 Aubin S. Beaugrand JBerteloot M. Boutrou RBuche PGontard N. . Plastics in a circular economy: Mitigating the ambiguity of widely-used terms from stakeholders consultation. Environ Sci Pol. (2022) 134:119–26. doi: 10.1016/j.envsci.2022.04.011 Kawashima N. Yagi TKojima K. How Do Bioplastics and Fossil-Based Plastics Play in a Circular Economy? Macromol Mater Eng. (2019) 304:1900383. doi: 10.1002/mame.201900383 Lambert S. Wagner M. Environmental performance of bio-based and biodegradable plastics: The road ahead. Chemical Society Reviews. (2017) 46:6855–71. doi: 10.1039/c7cs00149e EN 17228. European Standard EN 17228:2019 Plastics - Bio-based polymers, plastics, and plastics products - Terminology, characteristics and communication. (2019). Available at: https://www.en-standard.eu/search/?q=EN+17228%3A2019 Chen G-Q. A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem Soc Rev. (2009) 38:2434–46. doi: 10.1039/b812677c Zou X. Cheng CFeng JSong X. Lin MYang S-T. Biosynthesis of polymalic acid in fermentation: Advances and prospects for industrial application. Crit Rev Biotechnol. (2019) 39:408–21. doi: 10.1080/07388551.2019.1571008 Candido R. G. Godoy G. G. Gonçalves A. R. Characterization and application of cellulose acetate synthesized from sugarcane bagasse. Carbohydr Polym. (2017) 167:280–9. doi: 10.1016/j.carbpol.2017.03.057 Cywar R. M. Rorrer N. A. Hoyt C. B. Beckham G. T. Chen EY-X. Bio-based polymers with performance-advantaged properties. Nat Rev Materials. (2022) 7:83–103. doi: 10.1038/s41578-021-00363-3 Kopittke P. M. Menzies N. W. Wang P McKenna B. A. Lombi E. Soil and the intensification of agriculture for global food security. Environ Int. (2019) 132:105078. doi: 10.1016/j.envint.2019.105078 Pellis A. Malinconico MGuarneri A. Gardossi L. Renewable polymers and plastics: Performance beyond the green. Biotechnol. (2021) 60:146–58. doi: 10.1016/j.nbt.2020.10.003 Walker S. Rothman R. Life cycle assessment of bio-based and fossil-based plastic: A review. J Clean Prod. (2020) 261:121158. doi: 10.1016/j.jclepro.2020.121158 Donner M. Gohier R. Vries H.de (2020). A new circular business model typology for creating value from agro-waste. Sci Total Environ. 716,:137065. doi: 10.1016/j.scitotenv.2020.137065 Donner M. Verniquet A. Broeze J. Kayser K. Vries H.de. Critical success and risk factors for circular business models valorising agricultural waste and by-products. Resour Conserv Recycl. (2021) 165:105236. doi: 10.1016/j.resconrec.2020.105236 Vert M. Doi Y. Hellwich K.-H. Hess M. Hodge P. Kubisa P. (2012). Terminology for biorelated polymers and applications (IUPAC Recommendations 2012). Pure Appl Chem. 84, 377–410. doi: 10.1351/PAC-REC-10-12-04 Angellier-Coussy H. Guillard V. Buche PGontard N. Barquettes agro-sourcées à base de sous-produits des industries agro-alimentaires: Projet FP7 EcoBioCAP. Innovat Agronom. (2017) 58:61–7. Duque A. F. Oliveira C. S. S. Carmo I. T. D. Gouveia A. R. Pardelha F Ramos A. M. . Response of a three-stage process for PHA production by mixed microbial cultures to feedstock shift: Impact on polymer composition. New Biotechnol. (2014) 31:276–88. doi: 10.1016/j.nbt.2013.10.010 Kourmentza CPlácido J. Venetsaneas N. Burniol-Figols A. Varrone C. Gavala H. N. . Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production. Bioengineering. (2017)4: doi: 10.3390/bioengineering4020055 Koller M. Established and advanced approaches for recovery of microbial polyhydroxyalkanoate (PHA) biopolyesters from surrounding microbial biomass. The EuroBiotech J. (2020a) 4:113–26. doi: 10.2478/ebtj-2020-0013 Koller M. Niebelschütz H. Braunegg G. Strategies for recovery and purification of poly[(R)-3-hydroxyalkanoates] (PHA) biopolyesters from surrounding biomass. Eng Life Sci. (2013) 13:549–62. doi: 10.1002/elsc.201300021 Pagliano G. Galletti PSamorì CZaghini ATorri C. Recovery of Polyhydroxyalkanoates From Single and Mixed Microbial Cultures: A Review. Front Bioeng Biotechnol. (2021) 9:624021. doi: 10.3389/fbioe.2021.624021 Koller M. (Ed.) (2020b). The Handbook of Polyhydroxyalkanoates: Volume 3. Postsynthetic Treatment, Processing and Application. Taylor&Francis. Pérez-Rivero C. López-Gómez J. P. Roy I. A sustainable approach for the downstream processing of bacterial polyhydroxyalkanoates: State-of-the-art and latest developments. Biochem Eng J. (2019) 150:107283. doi: 10.1016/j.bej.2019.107283 Bossu JAngellier-Coussy H. Totee C. Matos M. Reis M. Guillard V. Effect of the Molecular Structure of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-3HV)) Produced from Mixed Bacterial Cultures on Its Crystallization and Mechanical Properties. Biomacromolecules. (2020) 21:4709–23. doi: 10.1021/acs.biomac.0c00826 Haque M. A. Priya A. Hathi Z. J. Qin Z-H Mettu SLin C. S. K. Advancements and current challenges in the sustainable downstream processing of bacterial polyhydroxyalkanoates. Curr Opin Green Sustain Chem. (2022) 36:100631. doi: 10.1016/j.cogsc.2022.100631 Arcos-Hernandez M. V. Laycock B. Pratt S. Donose B. C. Nikolić M. A. Luckman P. (2012). Biodegradation in a soil environment of activated sludge derived polyhydroxyalkanoate (PHBV). Polym Degrad Stab. 97, 2301–2312. doi: 10.1016/j.polymdegradstab.2012.07.035 David GMichel JGastaldi E. Gontard N. Angellier-Coussy H. How Vine Shoots as Fillers Impact the Biodegradation of PHBV-Based Composites. Int J Mol Sci. (2019) 21. doi: 10.3390/ijms21010228 Sashiwa HFukuda R. Okura TSato SNakayama A. Microbial Degradation Behavior in Seawater of Polyester Blends Containing Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Mar Drugs. (2018) 16. doi: 10.3390/md16010034 Emadian S. M. Onay T. T. Demirel B. Biodegradation of bioplastics in natural environments In: Waste Management, vol. 59. (New York, N.Y.): (2017). 526–36. doi: 10.1016/j.wasman.2016.10.006 Kumaravel SHema R. Lakshmi R. Production of Polyhydroxybutyrate (Bioplastic) and its Biodegradation by Pseudomonas Lemoignei and Aspergillus Niger. E-J Chem. (2010) 7:S536–42. doi: 10.1155/2010/148547 Lee K-M Gimore D. F. Huss M. J. Fungal Degradation of the Bioplastic PHB (Poly-3-hydroxy-butyric acid). J Polym Environ. (2006) 14:213. doi: 10.1007/s10924-006-0013-8 Meereboer K. W. Misra M Mohanty A. K. Review of recent advances in the biodegradability of polyhydroxyalkanoate (PHA) bioplastics and their composites. Green Chem. (2020) 22:5519–58. doi: 10.1039/D0GC01647K Roohi Z. M. R. Kuddus M. PHB (poly-β-hydroxybutyrate) and its enzymatic degradation. Polym Adv Technol. (2018) 29:30–40. doi: 10.1002/pat.4126 Weng Y-X Wang X-L Wang Y-Z. Biodegradation behavior of PHAs with different chemical structures under controlled composting conditions. Polym Testing. (2011) 30:372–80. doi: 10.1016/j.polymertesting.2011.02.001 EC 450/2009. Commission Regulation (EC) No 450/2009 of 29 May 2009 on active and intelligent materials and articles intended to come into contact with food. Official Journal of European Union. (2009) Available at: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32009R0450 Malhotra B. Keshwani A. Kharkwal H. Antimicrobial food packaging: Potential and pitfalls. Front Microbiol. (2015) 6:611. doi: 10.3389/fmicb.2015.00611 Yildirim S., Rocker B. Pettersen M. K., Nilsen-Nygaard J. Ayhan Z. Rutkaite R. Coma V. Active Packaging Applications for Food. Comprehen Rev Food Sci Food Safety Adv. (2017) doi: 10.1111/1541-4337.12322 Rüegg N. Röcker B. Yildirim S. Application of palladium-based oxygen scavenger to extend the mould free shelf life of bakery products. Food Packag Shelf Life. (2022) 31:100771. doi: 10.1016/j.fpsl.2021.100771 Aday M. S. Caner C. Rahvalı F. Effect of oxygen and carbon dioxide absorbers on strawberry quality. Postharvest Biol Technol. (2011) 62:179–87. doi: 10.1016/j.postharvbio.2011.05.002 Pantaleao IPintado MPocas M. Evaluation of two packaging systems for regional cheese. Food Chem. (2007) 102:481–7. doi: 10.1016/j.foodchem.2006.05.058 Wei H. Li L. Zhang T. Seidi FXiao H. Platinum-loaded dendritic mesoporous silica as novel ethylene scavenger to extend shelf life of banana (Musa nana). Food Chem. (2023) 424:136415. doi: 10.1016/j.foodchem.2023.136415 Hansen A. Å. Moen B. Rødbotten M. Berget I. Pettersen M. K. Effect of vacuum or modified atmosphere packaging (MAP) in combination with a CO2 emitter on quality parameters of cod loins (Gadus morhua). Food Packag Shelf Life.(2016) 9, 29–37. doi: 10.1016/j.fpsl.2016.05.005 Hempel A. W. O’Sullivan M. G. Papkovsky D. B. Kerry J. P. Use of smart packaging technologies for monitoring and extending the shelf-life quality of modified atmosphere packaged (MAP) bread: application of intelligent oxygen sensors and active ethanol emitters. Eur Food Res Technol. (2013) 237:117–24. doi: 10.1007/s00217-013-1968-z Sirocchi VDevlieghere F. Peelman NSagratini G. Maggi FVittori S. Effect of Rosmarinus officinalis L. Essential oil combined with different packaging conditions to extend the shelf life of refrigerated beef meat. Food Chem. (2017) 221:1069–76. doi: 10.1016/j.foodchem.2016.11.054 Graciano-Verdugo A. Z. Soto-Valdez H. Peralta E. Cruz-Zárate P. Islas-Rubio A. R. Sánchez-Valdes S. Migration of α-tocopherol from LDPE films to corn oil and its effect on the oxidative stability. Food Res Int. (2010) 43, 1073–1078. doi: 10.1016/j.foodres.2010.01.019 Van Boekel M. A. Kinetic Modeling of Food Quality: A Critical Review. Comp Rev Food Sci Food Safety. (2008) 7:144–58. doi: 10.1111/j.1541-4337.2007.00036.x Di Giuseppe FCoffigniez FAouf C. Guillard V. Torrieri E. Activated gallic acid as radical and oxygen scavenger in biodegradable packaging film. Food Packag Shelf. (2022) 31:100811. doi: 10.1016/j.fpsl.2022.100811 de F. S. M. Lopes P. S. Da Silva C. F. Yoshida C. M. P. Active packaging material based on buriti oil - Mauritia flexuosa L.f. (Arecaceae) incorporated into chitosan films. J Appl Polym Sci. (2016) 133:n/a-n/a. doi: 10.1002/app.43210 Mustapha F. A. Jai JNik Raikhan N. H. Sharif Z. Yusof N. M. Response surface methodology analysis towards biodegradability and antimicrobial activity of biopolymer film containing turmeric oil against Aspergillus niger. Food Control. (2019) 99:106–13. doi: 10.1016/j.foodcont.2018.12.042 Ramos M. Fortunati E., Beltrán A. Peltzer M. Cristofaro F. Visai L. . Properties of Poly (Lactic Acid)/Thymol/Nanoclay Composites. Polymers. (2020) 12. doi: 10.3390/polym12091878 Suwanamornlert PKerddonfag N. Sane AChinsirikul WZhou WChonhenchob V. Poly(lactic acid)/poly(butylene-succinate-co-adipate) (PLA/PBSA) blend films containing thymol as alternative to synthetic preservatives for active packaging of bread. Packag Shelf. (2020) 25:100515. doi: 10.1016/j.fpsl.2020.100515 Alparslan Y. Yapıcı H. H. Metin C. Baygar TGünlü A. Baygar T. Quality assessment of shrimps preserved with orange leaf essential oil incorporated gelatin. LWT. (2016) 72:457–66. doi: 10.1016/j.lwt.2016.04.066 Falleh H. Ben Jemaa M. Saada M. Ksouri R. Essential oils: A promising eco-friendly food preservative. Food Chem. (2020) 330:127268. doi: 10.1016/j.foodchem.2020.127268 Guo Q. Du G. Jia H. Fan Q. Wang Z. Gao Z. (2021). Essential oils encapsulated by biopolymers as antimicrobials in fruits and vegetables: A review. Food Biosci. 44:101367. doi: 10.1016/j.fbio.2021.101367 Seow Y. X. Yeo C. R. Chung H. L. Yuk H-G. Plant Essential Oils as Active Antimicrobial Agents. Crit Rev Food Sci Nutr. (2014) 54. doi: 10.1080/10408398.2011.599504 Corrales MFernández A. Han J. H. Chapter 7 - Antimicrobial Packaging Systems In: Han JH, editor. Food Science and Technology. Innovations in Food Packaging. (Second Edition) ed. San Diego: Academic Press (2014). 133–70. doi: 10.1016/B978-0-12-394601-0.00007-2 Gavara R. Lopez-Carballo G. Hernandez-Munoz P. Muriel-Galet VRamon Catala P. Cerisuelo J. Practical Guide to Antimicrobial Active Packaging. Retrieved from: Smithers Rapra Publishing (2015) Available at: https://books.google.fr/books?id=wKl5jgEACAAJ 1935/2004 EC. REGULATION (EC) No 1935/2004 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 27 October 2004 on materials and articles intended to come into contact with food and repealing Directives 80/590/EEC and 89/109/EEC. Official Journal of European Union. (2004) Available at: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32004R1935 Han J. H. ed. Innivations in Food Packaging Elsevier (2005). Nerin C. Silva F., Manso S. Becerril R. (2016). The Downside of Antimicrobial Packaging. In Antimicrobial Food Packaging (pp. 81–93). Elsevier. doi: 10.1016/B978-0-12-800723-5.00006-1 Almasi H. Jahanbakhsh Oskouie MSaleh A. A review on techniques utilized for design of controlled release food active packaging. Crit Rev Food Sci Nutr. (2021) 61:2601–21. doi: 10.1080/10408398.2020.1783199 Fu YDudley E. G. Antimicrobial-coated films as food packaging: A review. Compr Rev Food Sci Food Saf. (2021) 20:3404–37. doi: 10.1111/1541-4337.12769 Vasile CBaican M. Progresses in Food Packaging, Food Quality, and Safety-Controlled-Release Antioxidant and/or Antimicrobial Packaging. Molecules. (Basel, Switzerland), (2021) 26. doi: 10.3390/molecules26051263 Wicochea-Rodríguez J. D. Chalier PRuiz TGastaldi E. Active Food Packaging Based on Biopolymers and Aroma Compounds: How to Design and Control the Release. Front Chem. (2019) 7:398. doi: 10.3389/fchem.2019.00398 Vilela C. Kurek M. Hayouka Z. Röcker B. Yildirim S. Antunes M. D. C. (2018). A concise guide to active agents for active food packaging. Trends Food Sci Technol. 80, 212–222. doi: 10.1016/j.tifs.2018.08.006 Beltrán Sanahuja A. Valdés García A. New Trends in the Use of Volatile Compounds in Food Packaging. Polymers. (2021) 13. doi: 10.3390/polym13071053 Qie R. Zajforoushan Moghaddam S Thormann E. Dopamine-Assisted Layer-by-Layer Deposition Providing Coatings with Controlled Thickness, Roughness, and Functional Properties. ACS Omega. (2023) 8:2965–72. doi: 10.1021/acsomega.2c05620 Zhang C. Li Y. Wang P. Zhang H. Electrospinning of nanofibers: Potentials and perspectives for active food packaging. Compr Rev Food Sci Food Saf. (2020) 19:479–502. doi: 10.1111/1541-4337.12536 Modan E. M. Plaiasu A. G. Advantages and Disadvantages of Chemical Methods in the Elaboration of Nanomaterials. The Annals of “Dunarea De Jos” University of Galati. Fascicle IX, Metallurgy and Materials. Science. (2020) 43:53–60. doi: 10.35219/mms.2020.1.08 Hussein K. H. Park K.-M. Lee Y.-S. Woo J.-S. Kang B.-J. Choi K.-Y. (2017). New insights into the pros and cons of cross-linking decellularized bioartificial organs. Inter J Art Organs. 40, 136–141. doi: 10.5301/ijao.5000541 Westlake J. R. Tran M. W. Jiang Y. Zhang X. Burrows A. D. Xie M. Biodegradable Active Packaging with Controlled Release: Principles, Progress, and Prospects. ACS Food Sci Technol. (2022) 2:1166–83. doi: 10.1021/acsfoodscitech.2c00070 Becerril R., Nerín C. Silva F. Encapsulation Systems for Antimicrobial Food Packaging Components: An Update. Molecules. (2020) 25. doi: 10.3390/molecules25051134 Stoleru E. Brebu M. Stabilization Techniques of Essential Oils by Incorporation into Biodegradable Polymeric Materials for Food Packaging. Molecules.(2021) 26. doi: 10.3390/molecules26206307 Pedrós-Garrido S. Clemente I. Calanche J. B. Condón-Abanto S. Beltrán J. A. Lyng J. G. Whyte P. Antimicrobial activity of natural compounds against listeria spp. and their effects on sensory attributes in salmon (Salmo salar) and cod (Gadus morhua). Food Control. (2020) 107, 106768. doi: 10.1016/j.foodcont.2019.106768 Reis D. R. Ambrosi A. Di Luccio M. Encapsulated essential oils: A perspective in food preservation. Future Foods. (2022) 5:100126. doi: 10.1016/j.fufo.2022.100126 Zanetti M. Carniel T. K. Dalcanton F. dos Anjos R. S. Gracher Riella H. Araújo P. H.de (2018). Use of encapsulated natural compounds as antimicrobial additives in food packaging: A brief review. Trends Food Sci Technol, 81, 51–60. doi: 10.1016/j.tifs.2018.09.003 Kurek M. Laridon Y. Torrieri E. Guillard V. Pant A. Stramm C. (2017). A mathematical model for tailoring antimicrobial packaging material containing encapsulated volatile compounds. Innov Food Sci Emerg Technol. 42, 64–72. doi: 10.1016/j.ifset.2017.05.014 Nedovic V. Kalusevic A. Manojlovic V. Levic S. Bugarski B. An overview of encapsulation technologies for food applications. Proc Food Sci. (2011) 1:1806–15. doi: 10.1016/j.profoo.2011.09.265 Rezaei A. Fathi M. Jafari S. M. Nanoencapsulation of hydrophobic and low-soluble food bioactive compounds within different nanocarriers. Hydrocoll. (2019) 88:146–62. doi: 10.1016/j.foodhyd.2018.10.003 Bumbudsanpharoke N. Ko S. Nano-food packaging: An overview of market, migration research, and safety regulations. J Food Sci. (2015) 80:R910–23. doi: 10.1111/1750-3841.12861 Saad H. Ayed A. Srasra M. Attia S. Srasra E. Bouhtoury FC-E. . New Trends in Clay-Based Nanohybrid Applications: Essential Oil Encapsulation Strategies to Improve Their Biological Activity: 2 In: Oueslati W, editor. Nanoclay. Rijeka: IntechOpen (2022) doi: 10.5772/intechopen.106855 Ribeiro-Santos R. Andrade M. Melo N. R.de Sanches-Silva A. Use of essential oils in active food packaging: Recent advances and future trends. Trends Food Sci Techno. (2017) 61, 132–140. doi: 10.1016/j.tifs.2016.11.021 Topuz FUyar T. Antioxidant, antibacterial and antifungal electrospun nanofibers for food packaging applications. Food Res Int. (2020) 130:108927. doi: 10.1016/j.foodres.2019.108927 Zhang W. Jiang H. Rhim J-W. Cao J. Jiang W. Effective strategies of sustained release and retention enhancement of essential oils in active food packaging films/coatings. Food Chem. (2022) 367:130671. doi: 10.1016/j.foodchem.2021.130671 Abdul Rahman N. Applications of Polymeric Nanoparticles in Food Sector In: Siddiquee S Melvin GJH Rahman MM, eds. Nanotechnology: Applications in Energy, Drug and Food. Cham: Springer International Publishing (2019). 345–59. Rehman A. Jafari S. M. Aadil R M. Assadpour E. Randhawa M. A. Mahmood S. Development of active food packaging via incorporation of biopolymeric nanocarriers containing essential oils. Trends Food Sci Technol. (2020) 101:106–21. doi: 10.1016/j.tifs.2020.05.001 Balaguer M. P. Villanova J. Cesar G. Gavara R. Hernandez-Munoz P. Compostable properties of antimicrobial bioplastics based on cinnamaldehyde cross-linked gliadins. Chem Eng J. (2015) 262:447–55. doi: 10.1016/j.cej.2014.09.099 Cano A. I. Cháfer M. Chiralt A. González-Martínez C. Biodegradation behavior of starch-PVA films as affected by the incorporation of different antimicrobials. Polym Degrad Stab. (2016) 132:11–20. doi: 10.1016/j.polymdegradstab.2016.04.014 Piñeros-Hernandez D. Medina-Jaramillo C. López-Córdoba A. Goyanes S. Edible cassava starch films carrying rosemary antioxidant extracts for potential use as active food packaging. Food Hydrocollo. (2017) 63:488–95. doi: 10.1016/j.foodhyd.2016.09.034 Ketkaew S. Kasemsiri P. Hiziroglu S. Mongkolthanaruk W. Wannasutta R. Pongsa U. . Effect of Oregano Essential Oil Content on Properties of Green Biocomposites Based on Cassava Starch and Sugarcane Bagasse for Bioactive Packaging. J Polym Environ. (2018) 26:311–8. doi: 10.1007/s10924-017-0957-x Tampau A. González-Martínez C. Chiralt A. Release kinetics and antimicrobial properties of carvacrol encapsulated in electrospun poly-(ε-caprolactone) nanofibres. Application in starch multilayer films. Food Hydrocollo. (2018) 79:158–69. doi: 10.1016/j.foodhyd.2017.12.021 Tampau A. González-Martínez C. Chiralt A. Biodegradability and disintegration of multilayer starch films with electrospun PCL fibres encapsulating carvacrol. Polym Degrad Stab. (2020) 173:109100. doi: 10.1016/j.polymdegradstab.2020.109100 Arrieta M. P., Díez García A. López D. Fiori S. Peponi L. (2019). Antioxidant Bilayers Based on PHBV and Plasticized Electrospun PLA-PHB Fibers Encapsulating Catechin. Nanomaterials. 9. doi: 10.3390/nano9030346 Vostrejs P. Adamcová D. Vaverková M. D. Enev V. Kalina M. Machovsky M. (2020). Active biodegradable packaging films modified with grape seeds lignin. RSC Adv. 10, 29202–29213. doi: 10.1039/d0ra04074f Bonilla J. Poloni T. Sobral P. J. A. Active edible coatings with Boldo extract added and their application on nut products: reducing the oxidative rancidity rate. Int J Food Sci Technol. (2017) 53. doi: 10.1111/ijfs.13645 Bonilla J. Sobral P. J. A. Gelatin-chitosan edible film activated with Boldo extract for improving microbiological and antioxidant stability of sliced Prato cheese. Int J Food Sci Technol. (2018) 53. doi: 10.1111/ijfs.14032 Bonilla J. Sobral P. J. A. Disintegrability under composting conditions of films based on gelatin, chitosan and/or sodium caseinate containing boldo-of-Chile leafs extract. Int J Biol Macromol. (2020) 151:178–85. doi: 10.1016/j.ijbiomac.2020.02.051 Rech C. R. Da Silva Brabes K. C. Bagnara e Silva B. E. Bittencourt P. R. S. Koschevic M. T. Da Silveira T. F. S. (2020). Biodegradation of eugenol-loaded polyhydroxybutyrate films in different soil types. Case Stud Chem Environ Eng, 2, 100014. doi: 10.1016/j.cscee.2020.100014 Norcino L. B. Mendes J. F. Natarelli C. Manrich A. Oliveira J. E. Mattoso L. Pectin films loaded with copaiba oil nanoemulsions for potential use as bio-based active packaging. Food Hydrocoll. (2020) 106:105862. doi: 10.1016/j.foodhyd.2020.105862 Oberlintner A. Bajić M. Kalčíková G. Likozar B. Novak U. Biodegradability study of active chitosan biopolymer films enriched with Quercus polyphenol extract in different soil types. Environ Technol Innov. (2021) 21:101318. doi: 10.1016/j.eti.2020.101318 Ibrahim S. Elsayed H. Hasanin M. Biodegradable, Antimicrobial and Antioxidant Biofilm for Active Packaging Based on Extracted Gelatin and Lignocelluloses Biowastes. J Polym Environ. (2021) 29:472–82. doi: 10.1007/s10924-020-01893-7 He X. Li M. Gong X. Niu B. Li W. Biodegradable and antimicrobial CSC films containing cinnamon essential oil for preservation applications. Food Packag Shelf Life. (2021) 29:100697. doi: 10.1016/j.fpsl.2021.100697 Sadeghi A. Razavi S. M. A. Shahrampour D. Fabrication and characterization of biodegradable active films with modified morphology based on polycaprolactone-polylactic acid-green tea extract. Int J Biol Macromol. (2022) 205:341–56. doi: 10.1016/j.ijbiomac.2022.02.070, PMID: 35182564 Arrieta M. P., López J. Hernández A. Rayón E. (2014). Ternary PLA–PHB–Limonene blends intended for biodegradable food packaging applications. Eur Polym J. 50, 255–270. doi: 10.1016/j.eurpolymj.2013.11.009 Ramos M. Fortunati E., Peltzer M. Dominici F., Jiménez A. Del Garrigós M. C. Kenny J. M. (2014). Influence of thymol and silver nanoparticles on the degradation of poly(lactic acid) based nanocomposites: Thermal and morphological properties. Polym Degrad Stab. 108, 158–165. doi: 10.1016/j.polymdegradstab.2014.02.011 Yang W. Fortunati E., Dominici F., Giovanale G. Mazzaglia A. Balestra G. M. (2016). Effect of cellulose and lignin on disintegration, antimicrobial and antioxidant properties of PLA active films. Int J Biol Macromol. 89, 360–368. doi: 10.1016/j.ijbiomac.2016.04.068 Medina Jaramillo C. Gutiérrez T. J. Goyanes S. Bernal C. Famá L. Biodegradability and plasticizing effect of yerba mate extract on cassava starch edible films. Carbohydr Polym. (2016) 151:150–9. doi: 10.1016/j.carbpol.2016.05.025 Kim S. Baek S-K Go E. Song K. B. Application of Adzuki Bean Starch in Antioxidant Films Containing Cocoa Nibs Extract. Polymers. (2018) 10. doi: 10.3390/polym10111210 Lyu J. S. Lee J-S Han J. Development of a biodegradable polycaprolactone film incorporated with an antimicrobial agent via an extrusion process. Sci Rep. (2019) 9:20236. doi: 10.1038/s41598-019-56757-5 Villegas C. Arrieta M. P., Rojas A., Torres A. Faba S. Toledo M. J. (2019). PLA/organoclay bionanocomposites impregnated with thymol and cinnamaldehyde by supercritical impregnation for active and sustainable food packaging. Compos Part B. 176,:107336. doi: 10.1016/j.compositesb.2019.107336 Papadopoulou E. L. Paul U. C. Tran T. N. Suarato G. Ceseracciu L. Marras S. (2019). Sustainable Active Food Packaging from Poly(lactic acid) and Cocoa Bean Shells. ACS Appl Mater Interfaces. 11, 31317–31327. doi: 10.1021/acsami.9b09755 Ulloa P. A. Vidal J. Dicastillo C. Rodriguez F. Guarda A. Cruz R. M. S. [M. J.] (2019). Development of poly(lactic acid) films with propolis as a source of active compounds: Biodegradability, physical, and functional properties. J Appl Polym Sci, 136,:47090. doi: 10.1002/app.47090 Zabidi N., Nazri F. Tawakkal I. S. M. A. Basri M. S. M. Basha R. K. Othman S. H. (2022). Characterization of active and pH-sensitive poly(lactic acid) (PLA)/nanofibrillated cellulose (NFC) films containing essential oils and anthocyanin for food packaging application. Int J Biol Macromol. 212, 220–231. doi: 10.1016/j.ijbiomac.2022.05.116 Mendes J. F. Norcino L. B. Martins H. Manrich A. Otoni C. G. Carvalho E. (2020). Correlating emulsion characteristics with the properties of active starch films loaded with lemongrass essential oil. Food Hydrocollo. 100,:105428. doi: 10.1016/j.foodhyd.2019.105428 Zahan K. A. Azizul N. M. Mustapha M. Tong W. Y. Abdul Rahman M. S. Sahuri I. S. Application of bacterial cellulose film as a biodegradable and antimicrobial packaging material. Materials Today: Proceedings. (2020) 31:83–8. doi: 10.1016/j.matpr.2020.01.201 Patiño Vidal C. Luzi F. Puglia D., López-Carballo G. Rojas A., Galotto M. J. (2023). Development of a sustainable and antibacterial food packaging material based in a biopolymeric multilayer system composed by polylactic acid, chitosan, cellulose nanocrystals and ethyl lauroyl arginate. Food Packag Shelf Life. 36,:101050. doi: 10.1016/j.fpsl.2023.101050 Atarés L. Chiralt A. Essential oils as additives in biodegradable films and coatings for active food packaging. Trends Food Sci Technol. (2016) 48:51–62. doi: 10.1016/j.tifs.2015.12.001 Lagaron J. M. Lopez-Rubio A. Nanotechnology for bioplastics: opportunities, challenges and strategies. Trends Food Sci Technol. (2011) 22:611–7. doi: 10.1016/j.tifs.2011.01.007 Jenner K. J. Kreutzer G. Racine P. Persistency assessment and aerobic biodegradation of selected cyclic sesquiterpenes present in essential oils. Environ Toxicol Chem. (2011) 30:1096–108. doi: 10.1002/etc.492 Harder J. Heyen U. Probian C. Foß S. Anaerobic utilization of essential oils bydenitrifying bacteria. Biodegradation. (2000) 11:55–63. Suttinun O. Müller R. Luepromchai E. Cometabolic degradation of trichloroethene by Rhodococcus sp. Strain L4 immobilized on plant materials rich in essential oils. Appl Environ Microbiol. (2010) 76:4684–90. doi: 10.1128/AEM.03036-09 Lammi S. Gastaldi E. Gaubiac F. Angellier-Coussy H. How olive pomace can be valorized as fillers to tune the biodegradation of PHBV based composites. Polym Degrad Stab. (2019) 166:325–33. doi: 10.1016/j.polymdegradstab.2019.06.010