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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Neurosci.</journal-id>
<journal-title>Frontiers in Neuroscience</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Neurosci.</abbrev-journal-title>
<issn pub-type="epub">1662-453X</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fnins.2023.1125983</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Neuroscience</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Automatic facial coding predicts self-report of emotion, advertisement and brand effects elicited by video commercials</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name><surname>H&#x00F6;fling</surname> <given-names>T. Tim A.</given-names></name>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name><surname>Alpers</surname> <given-names>Georg W.</given-names></name>
<xref ref-type="corresp" rid="c001"><sup>&#x002A;</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/16846/overview"/>
</contrib>
</contrib-group>
<aff><institution>Department of Psychology, School of Social Sciences, University of Mannheim</institution>, <addr-line>Mannheim</addr-line>, <country>Germany</country></aff>
<author-notes>
<fn fn-type="edited-by"><p>Edited by: Giulia Cartocci, Sapienza University of Rome, Italy</p></fn>
<fn fn-type="edited-by"><p>Reviewed by: Marc Baker, University of Portsmouth, United Kingdom; Peter Lewinski, University of Oxford, United Kingdom</p></fn>
<corresp id="c001">&#x002A;Correspondence: Georg W. Alpers, <email>alpers@uni-mannheim.de</email></corresp>
<fn fn-type="other" id="fn004"><p>This article was submitted to Decision Neuroscience, a section of the journal Frontiers in Neuroscience</p></fn>
</author-notes>
<pub-date pub-type="epub">
<day>02</day>
<month>05</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="collection">
<year>2023</year>
</pub-date>
<volume>17</volume>
<elocation-id>1125983</elocation-id>
<history>
<date date-type="received">
<day>16</day>
<month>12</month>
<year>2022</year>
</date>
<date date-type="accepted">
<day>10</day>
<month>02</month>
<year>2023</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2023 H&#x00F6;fling and Alpers.</copyright-statement>
<copyright-year>2023</copyright-year>
<copyright-holder>H&#x00F6;fling and Alpers</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p></license>
</permissions>
<abstract>
<sec>
<title>Introduction</title>
<p>Consumers&#x2019; emotional responses are the prime target for marketing commercials. Facial expressions provide information about a person&#x2019;s emotional state and technological advances have enabled machines to automatically decode them.</p>
</sec>
<sec>
<title>Method</title>
<p>With automatic facial coding we investigated the relationships between facial movements (i.e., action unit activity) and self-report of commercials advertisement emotion, advertisement and brand effects. Therefore, we recorded and analyzed the facial responses of 219 participants while they watched a broad array of video commercials.</p>
</sec>
<sec>
<title>Results</title>
<p>Facial expressions significantly predicted self-report of emotion as well as advertisement and brand effects. Interestingly, facial expressions had incremental value beyond self-report of emotion in the prediction of advertisement and brand effects. Hence, automatic facial coding appears to be useful as a non-verbal quantification of advertisement effects beyond self-report.</p>
</sec>
<sec>
<title>Discussion</title>
<p>This is the first study to measure a broad spectrum of automatically scored facial responses to video commercials. Automatic facial coding is a promising non-invasive and non-verbal method to measure emotional responses in marketing.</p>
</sec>
</abstract>
<kwd-group>
<kwd>automatic facial coding</kwd>
<kwd>action units (AU)</kwd>
<kwd>FACS</kwd>
<kwd>facial expression</kwd>
<kwd>emotion</kwd>
<kwd>advertisement</kwd>
<kwd>brand</kwd>
<kwd>semiparametric additive mixed models</kwd>
</kwd-group>
<counts>
<fig-count count="3"/>
<table-count count="7"/>
<equation-count count="0"/>
<ref-count count="94"/>
<page-count count="15"/>
<word-count count="11007"/>
</counts>
</article-meta>
</front>
<body>
<sec id="S1" sec-type="intro">
<title>Introduction</title>
<p>Consumer neuroscience promises a better understanding of consumers&#x2019; emotions and attitudes with objective measures. Emotions play a central role in attitude formation (<xref ref-type="bibr" rid="B35">Ito and Cacioppo, 2001</xref>), information processing (<xref ref-type="bibr" rid="B42">Lemerise and Arsenio, 2000</xref>; <xref ref-type="bibr" rid="B28">Fraser et al., 2012</xref>), and decision-making in general (<xref ref-type="bibr" rid="B73">Slovic et al., 2007</xref>; <xref ref-type="bibr" rid="B58">Pittig et al., 2014</xref>). Hence, a direct measurement of emotional responses with advanced technologies might be key to understanding consumers&#x2019; behavior and decisions (<xref ref-type="bibr" rid="B2">Ariely and Berns, 2010</xref>; <xref ref-type="bibr" rid="B74">Solnais et al., 2013</xref>). In particular, emotions play a central role in marketing communications, such as video commercials to elicit desired advertisement and brand effects (<xref ref-type="bibr" rid="B1">Achar et al., 2016</xref>). Correspondingly, advertisements influence customers&#x2019; perception of brands which potentially moderates their purchase decisions and behaviors (<xref ref-type="bibr" rid="B59">Plassmann et al., 2012</xref>). Furthermore, consumers&#x2019; expectancies about a particular brand have a strong psychological impact because they can modulate consumption experience (<xref ref-type="bibr" rid="B49">McClure et al., 2004</xref>; <xref ref-type="bibr" rid="B71">Shiv et al., 2005</xref>). Neural activation patterns elicited by video commercials were previously investigated to either predict advertisement effectiveness beyond self-report with fMRI (<xref ref-type="bibr" rid="B7">Berns and Moore, 2012</xref>; <xref ref-type="bibr" rid="B27">Falk et al., 2012</xref>) and electroencephalogram (EEG) (<xref ref-type="bibr" rid="B22">Dmochowski et al., 2014</xref>; <xref ref-type="bibr" rid="B8">Boksem and Smidts, 2015</xref>) or to display latent emotional processes on a continues basis (<xref ref-type="bibr" rid="B54">Ohme et al., 2010</xref>; <xref ref-type="bibr" rid="B86">Vecchiato et al., 2011</xref>). However, such measures either require obtrusive research settings (fMRI tubes) or sensors attached to the scalp (EEG) and are, thus, not entirely non-invasive.</p>
<sec id="S1.SS1">
<title>Facial expression and emotion</title>
<p>Besides brain activity, emotional experiences also induce affective expressions (<xref ref-type="bibr" rid="B62">Sander et al., 2018</xref>; <xref ref-type="bibr" rid="B67">Scherer and Moors, 2019</xref>). Facial expression is the most investigated and predictive aspect of emotional expressions (<xref ref-type="bibr" rid="B66">Scherer and Ellgring, 2007</xref>; <xref ref-type="bibr" rid="B60">Plusquellec and Denault, 2018</xref>). In comparison to measures of brain activity, facial expressions responses are typically video-based, which requires no measurement preparation or application, can be obtained in ecologically valid environments, and is even applicable to online research. In order to capture emotionally relevant information from the whole face, researchers have heavily relied on observation techniques such as the Facial Action Coding System (FACS) to score intensity estimates of single facial movements called Action Units (AU) (<xref ref-type="bibr" rid="B25">Ekman et al., 2002</xref>; <xref ref-type="bibr" rid="B47">Mauss and Robinson, 2009</xref>). FACS is an extensive coding manual which allows for a very detailed description of facial responses through the combination of AU and shows good to excellent inter-rater reliabilities (<xref ref-type="bibr" rid="B64">Sayette et al., 2001</xref>). However, human FACS coding results in low scaling resolution of AU intensities and it is very time consuming (<xref ref-type="bibr" rid="B69">Schulte-Mecklenbeck et al., 2017</xref>).</p>
<p>Although there are several important theories that explain specific aspects of emotional facial expressions [for an overview, see <xref ref-type="bibr" rid="B3">Barrett et al. (2019)</xref>], there are currently only two relevant empirical approaches that map combinations of specific AUs to specific emotional states. On the one side, basic emotion theory predicts robust AU patterns that cohere with a limited amount of distinct emotion categories (i.e., joy, anger, disgust, sadness, fear, and surprise; e.g., <xref ref-type="bibr" rid="B26">Ekman et al., 1987</xref>). However, there is evidence for universal facial expressions beyond six basic emotions (<xref ref-type="bibr" rid="B18">Cordaro et al., 2018</xref>, <xref ref-type="bibr" rid="B17">2020</xref>; <xref ref-type="bibr" rid="B36">Keltner et al., 2019</xref>; <xref ref-type="bibr" rid="B19">Cowen et al., 2021</xref>). Moreover, there are spontaneous emotional responses that are be much more variable and less universal for a distinct AU pattern (<xref ref-type="bibr" rid="B24">Dur&#x00E1;n and Fern&#x00E1;ndez-Dols, 2021</xref>; <xref ref-type="bibr" rid="B41">Le Mau et al., 2021</xref>; <xref ref-type="bibr" rid="B78">Tcherkassof and Dupr&#x00E9;, 2021</xref>). On the other side, componential process theory predicts several appraisal dimensions that elicit specific AU combinations like valence, novelty, and control (<xref ref-type="bibr" rid="B62">Sander et al., 2018</xref>; <xref ref-type="bibr" rid="B68">Scherer et al., 2018</xref>, <xref ref-type="bibr" rid="B65">2021</xref>).</p>
<p>Although electromyography (EMG) research extensively measured corrugator and zygomaticus activity to approximate a valence dimension (e.g., <xref ref-type="bibr" rid="B34">H&#x00F6;fling et al., 2020</xref>), the investigation of other components in this theory is still preliminary. Hence, there is currently no consensus about the link between meaningful AU combinations regardless of the assumption of dimensional or categorial underlying emotional processes.</p>
</sec>
<sec id="S1.SS2">
<title>Validity of AFC</title>
<p>Recent advances in technology have enabled the measurement of facial expressions to obtain emotion-associated parameters through automatic facial coding based on machine-learning (AFC; <xref ref-type="bibr" rid="B56">Pantic and Rothkrantz, 2003</xref>; <xref ref-type="bibr" rid="B16">Cohn and Sayette, 2010</xref>). AFC parameters include separate facial movements, such as Action Units derived from the Facial Action Coding System on the one side and integrated &#x201C;emotion-scores&#x201D; such as joy or anger estimations, on the other side. There is evidence that AU parameters estimated by AFC correspond with estimates of human FACS raters between 71 and 93%, depending on the specific measurement system (<xref ref-type="bibr" rid="B4">Bartlett et al., 1999</xref>; <xref ref-type="bibr" rid="B81">Tian et al., 2001</xref>; <xref ref-type="bibr" rid="B72">Skiendziel et al., 2019</xref>; <xref ref-type="bibr" rid="B70">Seuss et al., 2021</xref>). Moreover, AFC classifies basic emotional facial expressions with impressive accuracy in prototypical facial expressions in pictures (<xref ref-type="bibr" rid="B44">Lewinski et al., 2014a</xref>; <xref ref-type="bibr" rid="B43">Lewinski, 2015</xref>; <xref ref-type="bibr" rid="B5">Beringer et al., 2019</xref>; <xref ref-type="bibr" rid="B39">K&#x00FC;ntzler et al., 2021</xref>) as well as videos (<xref ref-type="bibr" rid="B48">Mavadati et al., 2013</xref>; <xref ref-type="bibr" rid="B93">Yitzhak et al., 2017</xref>; <xref ref-type="bibr" rid="B13">Calvo et al., 2018</xref>; <xref ref-type="bibr" rid="B38">Krumhuber et al., 2021</xref>).</p>
<p>While attempts to validate this innovative technology mainly focused on highly standardized and prototypical emotional facial expressions in the past, the number of validation studies that approximate more naturalistic or spontaneous facial expressions is still preliminary. AFC is less accurate in the detection of less intense and more naturalistic expressions (<xref ref-type="bibr" rid="B10">B&#x00FC;denbender et al., 2023</xref>), which is also a commonly observed pattern in human emotion recognition (<xref ref-type="bibr" rid="B38">Krumhuber et al., 2021</xref>). Some studies find evidence that AFC can be transferred to the facial expressions of na&#x00EF;ve participants that mimic emotional facial expressions in a typical laboratory setting (<xref ref-type="bibr" rid="B76">St&#x00F6;ckli et al., 2018</xref>; <xref ref-type="bibr" rid="B63">Sato et al., 2019</xref>; <xref ref-type="bibr" rid="B32">H&#x00F6;fling et al., 2022</xref>). To a limited extent, AFC detects highly unstandardized emotional facial expressions of professional actors depicted in movies (<xref ref-type="bibr" rid="B39">K&#x00FC;ntzler et al., 2021</xref>). Furthermore, AFC can also track spontaneous emotional responses toward pleasant scenes, where AFC parameters correlate with emotional self-report and direct measures of muscle activity with EMG (<xref ref-type="bibr" rid="B34">H&#x00F6;fling et al., 2020</xref>). However, AFC is not sensitive to very subtle emotional responses, particularly if participants are motivated to suppress or control their facial responses (<xref ref-type="bibr" rid="B33">H&#x00F6;fling et al., 2021</xref>). Taken together, there is evidence that AFC validly captures spontaneous emotional states in a typical laboratory setting, especially for pleasant emotional responses.</p>
</sec>
<sec id="S1.SS3">
<title>AFC and advertisement</title>
<p>According to the affect-transfer hypothesis (<xref ref-type="bibr" rid="B9">Brown and Stayman, 1992</xref>), there is evidence for a relationship between consumers&#x2019; emotional responses to advertisement stimuli and the subsequently elicited advertisement and brand effects. In this model, an advertisement elicits emotional responses which influence the attitude toward the advertisement (i.e., advertisement likeability). In the following step, a favorable ad likeability leads to changes in the attitude toward the brand (brand likeability), which increases the purchase intention of products and services of a particular brand in the final step of this process model.</p>
<p>AFC has been used successfully to predict the effects of video commercials&#x2019; on the subsequent processes of this advertisement and brand effect framework, such as the self-reported emotional response, advertisement likeability, as well as brand likeability and purchase intention. In the domain of political influencing, AFC measures have been shown to correspond with emotional self-report of pleasant (<xref ref-type="bibr" rid="B46">Mahieu et al., 2019</xref>) as well as unpleasant advertisements (<xref ref-type="bibr" rid="B30">Fridkin et al., 2021</xref>). They are also predictive to measure intended emotional responses in an <italic>a priori</italic> defined target audience (<xref ref-type="bibr" rid="B55">Otamendi and Sutil Mart&#x00ED;n, 2020</xref>). Accordingly, AFC of smiling intensity correlates with advertisement likeability (<xref ref-type="bibr" rid="B45">Lewinski et al., 2014b</xref>; <xref ref-type="bibr" rid="B50">McDuff et al., 2014</xref>, <xref ref-type="bibr" rid="B51">2015</xref>), brand likeability (<xref ref-type="bibr" rid="B45">Lewinski et al., 2014b</xref>), and the purchase intentions of advertised brands (<xref ref-type="bibr" rid="B79">Teixeira et al., 2014</xref>; <xref ref-type="bibr" rid="B51">McDuff et al., 2015</xref>). Furthermore, increased smiling was also found to reduce zapping behavior (<xref ref-type="bibr" rid="B92">Yang et al., 2014</xref>; <xref ref-type="bibr" rid="B14">Chen et al., 2016</xref>), decrease attention dispersion (<xref ref-type="bibr" rid="B80">Teixeira et al., 2012</xref>), and predict long-term attitude changes (<xref ref-type="bibr" rid="B31">Hamelin et al., 2017</xref>). Taken together, there is evidence that AFC of smiling predicts several steps of the advertisement and brand effects proposed by the affect-transfer hypothesis. In contrast to AFC, approaches to measure advertisement effects with human FACS were less successful (e.g., <xref ref-type="bibr" rid="B21">Derbaix, 1995</xref>).</p>
</sec>
<sec id="S1.SS4">
<title>Research gaps and overview</title>
<p>The scientific knowledge regarding a direct link between emotional facial expression on the one side and advertisement or brand effects on the other side is very limited due to the following reasons: First, it is unclear whether facial expressions significantly predict advertisement and brand effects beyond relevant precursor self-report dimensions. Such quantification is only possible if statistical models of facial expression parameters are compared with models that control for relevant self-report, which has not been investigated in previous research. Second, relevant research mainly used integrated parameters for joy or entertainment, relying almost exclusively on measurements of smiling (AU12). Reporting such integrated scores serves a lower level of scientific transparency in comparison to a description with AU terminology because it is largely unknown how AFC classifiers are trained and, correspondingly, how integrated parameters are estimated. As an additional consequence, it is unclear how different AUs that are relevant for emotional facial expressions ensembles to predict advertisement and brand effects of video commercials&#x2019; effectiveness beyond smiling. Third, there is no consensus on whether the degree of amusement or entertainment in video commercials shows a linear or non-linear relationship on branding effects: While <xref ref-type="bibr" rid="B45">Lewinski et al. (2014b)</xref> report a weak linear relationship between AFC of smiling and brand effects, <xref ref-type="bibr" rid="B79">Teixeira et al. (2014)</xref> report an inverted u-shaped relationship between AFC of smiling and brand effects. Finally, there is no study available that investigates the relationship between emotional facial expressions and all relevant steps of advertisement and brand effects according to the affect-transfer hypothesis in a within-subject study design.</p>
<p>In order to close these existing research gaps, we investigated the predictive value of facial expressions while viewing video commercials to forecast all subsequent components of the affect-transfer model of advertisement and brand effects: emotion ratings, ad likeability, changes between pre- and post-measurements of brand likeability, and purchase intention. To this end, we broaden the spectrum of analyzed movements in the face to 20 AU that can be measured with a state-of-the-art AFC algorithm to predict relevant outcome criteria (see <xref ref-type="fig" rid="F1">Figure 1</xref> for an overview of measured AUs and self-report ratings). In addition, we aim to determine non-linearities between facial expressions and self-report with semi-parametric additive mixed models that can account for non-linear effects (<xref ref-type="bibr" rid="B89">Wood, 2006</xref>; <xref ref-type="bibr" rid="B91">Wood et al., 2013</xref>; <xref ref-type="bibr" rid="B90">Wood and Scheipl, 2020</xref>). We expect strong relationships between self-reported emotion ratings and advertisement likeability and changes in brand likeability as well as purchase intention. In order to collect data from a variety of industries and emotional content, we established different stimulus groups which only differed in terms of advertisement videos and corresponding brand stimuli. This is the first study that investigates the relationship between emotional facial expressions measured by artificial intelligence and all relevant components of advertisement and brand effectiveness proposed by the affect-transfer framework.</p>
<fig id="F1" position="float">
<label>FIGURE 1</label>
<caption><p>Overview of the measured Action Units and hypothesized relations to self-report ratings. Depicted is a happy facial expression from the ADFES inventory (model F04; <xref ref-type="bibr" rid="B83">van der Schalk et al., 2011</xref>).</p></caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fnins-17-1125983-g001.tif"/>
</fig>
</sec>
</sec>
<sec id="S2" sec-type="materials|methods">
<title>Materials and methods</title>
<sec id="S2.SS1">
<title>Participants</title>
<p>A total of 257 volunteers were randomly assigned to one of eight stimulus groups. General exclusion criteria were age under 18, acute psychoactive medication use, acute mental disorder episode, severe somatic disease, and wearing glasses. Participants with corrected-to-normal vision were asked to wear contact lenses during the experiment. After visual inspection of the analyzed videos, data from 38 participants were excluded because of face detection problems of the AFC software (e.g., partially covered face with the hand, scarfs, and other accessories or large body movements that lead to false face detection). Hence, we only used data from participants with good recording and analysis quality, resulting in an overall sample of 219 (115 females) participants of mainly Caucasian descent. Age ranged from 19 to 58 years (<italic>M</italic> = 23.79, <italic>SD</italic> = 5.22). All participants received compensation of either 8&#x20AC; or student course credit, and they signed informed consent before the data collection. University Research Ethics Committee approved the experiment.</p>
</sec>
<sec id="S2.SS2">
<title>Questionnaires</title>
<p>Participants filled in various questionnaires to compare relevant states and traits related to emotional responsiveness and expressivity to ensure comparability between experimental groups. After a socio-demographic questionnaire (e.g., gender, age, and educational level), the Social Interaction Anxiety Scale (SIAS; <xref ref-type="bibr" rid="B75">Stangier et al., 1999</xref>), the State-Trait Anxiety Inventory (STAI State and STAI Trait; <xref ref-type="bibr" rid="B40">Laux et al., 1981</xref>), the Positive-and-Negative-Affect-Schedule (PANAS PA and PANAS NA; <xref ref-type="bibr" rid="B37">Krohne et al., 1996</xref>), the Self-Rating Depression Scale (SDS; <xref ref-type="bibr" rid="B94">Zung, 1965</xref>), the Berkley Expressivity Questionnaire (BEQ; <xref ref-type="bibr" rid="B52">Mohiyeddini et al., 2008</xref>), and the Behavioral Inhibition and Activation Scale (BIS and BAS; <xref ref-type="bibr" rid="B77">Strobel et al., 2001</xref>) were administered before starting the main experiment.</p>
</sec>
<sec id="S2.SS3">
<title>Study design and procedure</title>
<p>Following informed consent and completion of the questionnaires, participants were seated in front of a computer screen. In order to collect data from a variety of industries and emotional content, we established eight different stimulus groups to which participants were randomly assigned. These groups only differed in terms of advertisement videos and corresponding brand stimuli; all other aspects remained constant between groups. Before the main experiment started, participants were instructed to maintain a neutral facial expression for 10 s, which was later utilized to calibrate the AFC analysis individually. The main experiment comprised three experimental blocks: pre-evaluation of brands, viewing video advertisements and evaluation, and post-evaluation of brands.</p>
<p>The pre- and post-evaluations of brands were set up identically, and hence, effects on brands elicited by the video advertisement can be traced by pre-post rating changes. The logo of the eight advertised brands was also presented in both brand evaluation blocks, each started with a 1 s fixation cross. The presentation duration of the brand logos lasted until participants decided when to proceed with several ratings of a specific brand by pressing the space bar. All scales were presented as a nine-point semantic differential. Participants rated their familiarity with the brand (1 = familiar and 9 = unfamiliar), brand likeability (1 = like and 9 = dislike; 1 = good and 9 = bad), and brand purchase intention (1 = probable purchase and 9 = unprobeable purchase; 1 = purchasing definitely and 9 = purchasing definitely not) after the brand presentation. All advertisements were indicated by a 1 s fixation cross in the advertisement block. After watching a particular video, each video was rated on nine-point semantic differentials containing advertisement familiarity (1 = familiar and 9 = unfamiliar) and advertisement likeability (1 = like and 9 = dislike; 1 = good and 9 = bad). In addition, participants rated how they felt during each video presentation on several one-item emotion scales (i.e., joy, sadness, anger, disgust, fear, surprise; 1 = strong emotion and 9 = no emotion) immediately after the video presentation. All scales were inverted to improve the readability of the results. If two items were used to measure a construct (i.e., advertisement likeability, brand likeability, brand purchase intention), the average of both items would have been calculated. Internal consistencies were excellent for these three scales (Cronbach&#x2019;s &#x03B1; &#x003E; 0.90).</p>
</sec>
<sec id="S2.SS4">
<title>Stimulus material</title>
<p>Each group watched eight different commercials and corresponding brand logos in randomized order. The video material was selected from the list of award-winning commercials at the Cannes Lions International Festival of Creativity between 2016 and 2017. The videos were counterbalanced between groups in terms of video duration, familiarity with the brand, familiarity with the video, and emotional content (see also Section &#x201C;Results&#x201D;). Appendix 1 contains all commercial video advertisements sorted by groups. Two other non-commercial video advertisements per group were presented but not included in the analysis because the brand ratings did not apply to the non-commercial section.</p>
</sec>
<sec id="S2.SS5">
<title>Apparatus and measurements</title>
<p>High-precision software (E-Prime; Version 2.0.10; Psychology Software Tools, Pittsburgh, PA, USA) was used to run the experiment. Stimuli were shown centrally on a 19-inch monitor with a resolution of 1,024 &#x00D7; 768, approximately 70 cm away from the participant. Optimal illumination with diffused frontal light was maintained throughout. Videos of participants&#x2019; faces were recorded with a Logitech HD C615 web camera placed above the computer screen. Videos were processed off-line with FaceReader Software (FR; Version 7.0, Noldus Information Technology) and further analyzed with Observer XT (Version 12.5, Noldus Information Technology). Furthermore, Observer XT synchronized stimulus onset trigger from E-Prime and the video recordings. FR is a visual pattern classifier based on deep learning approaches and extracts visual features from videos frame by frame. In accordance with neuro-computational models of human face processing (<xref ref-type="bibr" rid="B20">Dailey et al., 2002</xref>; <xref ref-type="bibr" rid="B12">Calder and Young, 2005</xref>), FR detects facial configurations in the following steps (<xref ref-type="bibr" rid="B85">Van Kuilenburg et al., 2005</xref>, <xref ref-type="bibr" rid="B84">2008</xref>): (1) The <italic>Cascade classifier algorithm</italic> finds the position of the face (<xref ref-type="bibr" rid="B88">Viola and Jones, 2004</xref>). (2) Face textures are normalized and the <italic>active appearance model</italic> synthesizes a digital face model representing facial structure with over 500 location points. (3) Compressed distance information is then transmitted to an artificial neural network. (4) Finally, the artificial neural network connects these scores with relevant emotional labels through supervised training with over 10,000 samples (pictures) of emotional faces to classify the relative intensity of a given facial configuration. As a result, FR estimates activity of 20 AU, which includes AU01 Inner Brow Raiser, AU02 Outer Brow Raiser, AU04 Brow Lowerer, AU05 Upper Lid Raiser, AU06 Cheek Raiser, AU07 Lid Tightener, AU09 Nose Wrinkler, AU10 Upper Lid Raiser, AU12 Lip Corner Pull, AU14 Dimpler, AU15 Lip Corner Depressor, AU17 Chin Raiser, AU18 Lip Puckerer, AU20 Lip Stretcher, AU23 Lip Tightener, AU24 Lip Pressor, AU25 Lips Part, AU26 Jaw Drop, AU27 Mouth Stretch, and AU43 Eyes Closed. The estimated parameter of each AU ranges from 0 to 1. FR measures were calibrated per participant based on the baseline measurement at the beginning of the experiment. &#x201C;East-Asian&#x201D; or &#x201C;elderly&#x201D; face models were presented instead of the general face model as recommended by the user manual. For the duration of each video, the mean and peak activity for all AUs were exported and analyzed.</p>
</sec>
<sec id="S2.SS6">
<title>Data reduction and analysis</title>
<p>Across all participants (<italic>N</italic> = 219) and commercial video stimuli (<italic>n</italic> = 64; eight per participant), 1,752 data points were collected on advertisements and corresponding brand ratings. Our data analysis required several steps: analysis of participant characteristics, aggregation of video- and brand-wise means for analysis of stimulus characteristics, and also to report correlations between facial expression parameters and relevant self-report ratings, as well as semi-parametric mixed additive regression models to predict emotion, advertisement and brand effects based on facial expression parameters.</p>
<p>First, we calculated ANOVA with the factor stimulus group (eight groups) to determine differences in participant characteristics separately for STAI State, STAI Trait, SIAS, BIS, BAS, PANAS PA, PANAS NA, BEQ, and SDS. In addition, we report differences in gender distribution in stimulus groups with the Chi-Squared test.</p>
<p>Second, we calculated pre-post difference scores for brand likeability and brand purchase intention and averaged relevant self-report ratings and AU parameters separately for each video and the corresponding brand. On the one side, we determined differences in stimulus characteristics based on stimulus-wise averages for the stimulus groups with ANOVA for video duration, brand familiarity (pre-rating), video familiarity, and emotion ratings (i.e., joy, sadness, anger, fear, disgust, and surprise). We applied a Greenhouse&#x2013;Geisser correction for ANOVA when appropriate. Eta-squared (&#x03B7;<sup>2</sup>) is reported as an effect size for F-tests (&#x03B7;<sup>2</sup> &#x2265; 0.01 small; &#x03B7;<sup>2</sup> &#x2265; 0.06 medium; &#x03B7;<sup>2</sup> &#x2265; 0.14 large; <xref ref-type="bibr" rid="B57">Pierce et al., 2004</xref>).</p>
<p>Third, we identified AU that showed zero or near-zero variance to exclude AU that will not contribute to the prediction of relevant outcomes. This exclusion criterion applied for eight AU, in particular, AU01 Inner Brow Raiser, AU02 Outer Brow Raiser, AU09 Nose Wrinkler, AU10 Upper Lid Raiser, AU18 Lip Puckerer, AU20 Lip Stretcher, AU26 Jaw Drop, and AU27 Mouth Stretch, which were excluded from further analysis. Further, we report spearman&#x2019;s rho correlations between relevant AU and self-report based on averages for each video and the corresponding brand. Effect sizes were interpreted following <xref ref-type="bibr" rid="B15">Cohen (1988)</xref>: <italic>r</italic> &#x2265; 0.1 small, <italic>d</italic> &#x2265; 0.3 medium, and <italic>d</italic> &#x2265; 0.5 large.</p>
<p>Fourth, all self-report ratings were z-transformed on the group level for better interpretability and comparability of the effects. To resolve the limitation of the one-item scale of emotion ratings for joy, z-scores are calculated based on individual participant ratings. In contrast to self-report ratings, AU variables were not transformed in any way because of their scale properties (e.g., exact zero-point) and correspondence to the intensity measurement of the Facial Action Coding System: Values from &#x003E;0.00&#x2013;0.16 are classified as trace (E), 0.16&#x2013;0.26 as slight (D), 0.26&#x2013;0.58 as pronounced (C), 0.58&#x2013;0.90 as severe (B), and 0.90&#x2013;1 as max intensity (A).</p>
<p>Fifth, as predictive models, we carried out semi-parametric additive mixed modeling with the R-package &#x201C;gamm4&#x201D; (<xref ref-type="bibr" rid="B90">Wood and Scheipl, 2020</xref>). In the first step, we developed a basic model controlling gender and stimuli as fixed factors and the participants and stimulus group as random factors. Next, we calculated main effect models for all AU separately for peak and mean activity to predict joy ratings, advertisement likeability, brand likeability change, and brand purchase intention change. Furthermore, we estimated the combined effect of AU and relevant rating scales to determine the relative predictiveness of AFC versus self-report resulting in 17 independent models. Visualization of the most substantial effect patterns is presented as smoothed effect plots with 95% confidence intervals. We report <italic>R<sup>2</sup><sub><italic>adj</italic></sub></italic>, Akaike-Information-Criterion (<italic>AIC</italic>), and Bayesian-Information-Criterion (BIC) as a goodness of fit indices (<xref ref-type="bibr" rid="B11">Burnham and Anderson, 2004</xref>). According to <xref ref-type="bibr" rid="B15">Cohen (1988)</xref>, we interpreted the adjusted <italic>R</italic><sup>2</sup> &#x2265; 0.01 as small, <italic>R</italic><sup>2</sup> &#x2265; 0.09 as moderate, and <italic>R</italic><sup>2</sup> &#x2265; 0.25 as a large proportion of explained variance of each predictive model. A large change in model fit was interpreted by an absolute change of 10 for AIC and BIC. The significance level was always set to &#x03B1; = 0.05.</p>
</sec>
</sec>
<sec id="S3" sec-type="results">
<title>Results</title>
<sec id="S3.SS1">
<title>Manipulation checks</title>
<sec id="S3.SS1.SSS1">
<title>Questionnaire group differences</title>
<p>Participants were randomly assigned to one of eight groups with different stimulus materials. Appendix 2 shows descriptive statistics of the questionnaires separately for the groups. There were no significant differences between groups regarding STAI State, STAI Trait, SIAS, BIS, BAS, PANAS PA, PANAS NA, BEQ, SDS, and gender distribution. Hence, no meaningful differences in participant characteristics between groups can be reported.</p>
</sec>
<sec id="S3.SS1.SSS2">
<title>Stimulus group differences and stimulus emotion characteristics</title>
<p>Appendix 1 shows descriptive statistics for all advertisement videos and averaged scores of relevant brand and advertisement evaluations. Comparison of the different stimulus groups revealed no significant differences for video duration, <italic>F</italic>(7, 56) = 0.32, <italic>p</italic> = 0.944, &#x03B7;<sup>2</sup> = 0.04, brand familiarity (pre-rating), <italic>F</italic>(7, 56) = 0.20, <italic>p</italic> = 0.985, &#x03B7;<sup>2</sup> = 0.02, video familiarity, <italic>F</italic>(7, 56) = 1.62, <italic>p</italic> = 0.148, &#x03B7;<sup>2</sup> = 0.17, joy, <italic>F</italic>(7, 56) = 1.58, <italic>p</italic> = 0.162, &#x03B7;<sup>2</sup> = 0.17, sadness, <italic>F</italic>(7, 56) = 0.09, <italic>p</italic> = 0.999, &#x03B7;<sup>2</sup> = 0.01, anger, <italic>F</italic>(7, 56) = 0.93, <italic>p</italic> = 0.489, &#x03B7;<sup>2</sup> = 0.10, fear, <italic>F</italic>(7, 56) = 0.95, <italic>p</italic> = 0.476, &#x03B7;<sup>2</sup> = 0.11, disgust, <italic>F</italic>(7, 56) = 0.91, <italic>p</italic> = 0.503, &#x03B7;<sup>2</sup> = 0.10, and surprise ratings, <italic>F</italic>(7, 56) = 1.22, <italic>p</italic> = 0.299, &#x03B7;<sup>2</sup> = 0.13. Hence, no meaningful differences in stimulus characteristics between groups were found.</p>
<p>Importantly, different videos elicited different emotions, <italic>F</italic>(5, 315) = 166.59, <italic>p</italic> &#x003C; 0.001, &#x03B7;<sup>2</sup> = 0.73. While participants reported generally higher amounts of joy (<italic>M</italic> = 5.42, SD = 1.43) and surprise (<italic>M</italic> = 4.33, SD = 1.01), other emotions were reported substantially less (sadness: <italic>M</italic> = 2.15, SD = 1.33; anger: <italic>M</italic> = 1.79, SD = 0.60; fear: <italic>M</italic> = 1.58, SD = 0.61; disgust: <italic>M</italic> = 1.65, SD = 0.89) and, hence, were not included in the main analysis (i.e., semiparametric models of AU activity).</p>
</sec>
</sec>
<sec id="S3.SS2">
<title>Correlations between self-reports and facial expressions</title>
<p>Spearman correlations based on unstandardized average values per stimulus between emotion, advertisement, brand ratings, and AU intensity for mean and peak activity over the video duration are reported in <xref ref-type="table" rid="T1">Table 1</xref>. There were strong and positive correlations between facial expression measures of AU6, AU12, and AU25 for ratings of joy as well as surprise for both mean and peak AU activity. Although feelings of surprise can be elicited by pleasant to unpleasant emotional events, the advertisements presented in this study elicited the same correlational patterns of facial activity for higher surprise and higher joy ratings. Therefore, these two emotion ratings might be confounded in the present study design, probably because the videos mainly triggered pleasant emotions. Hence, we focused on joy ratings as a self-report measure of emotion elicited by video commercials in the main analysis (i.e., semiparametric models of AU activity).</p>
<table-wrap position="float" id="T1">
<label>TABLE 1</label>
<caption><p>Spearman correlations based on unstandardized average values per stimulus between self-report ratings and Action Unit (AU) activity (mean and peak).</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Joy</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Surprise</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Anger</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Fear</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Disgust</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Sadness</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Ad like</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Brand like</td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;">Purchase intention</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Ad like</td>
<td valign="top" align="center"><bold>0</bold>.<bold>75</bold></td>
<td valign="top" align="center">&#x2013;0.06</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>42</bold></td>
<td valign="top" align="center">&#x2013;0.19</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>48</bold></td>
<td valign="top" align="center">0.18</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td valign="top" align="left">Brand like</td>
<td valign="top" align="center">0.29</td>
<td valign="top" align="center">&#x2013;0.01</td>
<td valign="top" align="center">&#x2013;0.28</td>
<td valign="top" align="center">&#x2013;0.19</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>32</bold></td>
<td valign="top" align="center">&#x2013;0.08</td>
<td valign="top" align="center"><bold>0</bold>.<bold>33</bold></td>
<td/>
<td/>
</tr>
<tr>
<td valign="top" align="left">Purchase intention</td>
<td valign="top" align="center">0.29</td>
<td valign="top" align="center">&#x2013;0.21</td>
<td valign="top" align="center">&#x2013;0.22</td>
<td valign="top" align="center">&#x2013;0.18</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>40</bold></td>
<td valign="top" align="center">&#x2013;0.03</td>
<td valign="top" align="center"><bold>0</bold>.<bold>43</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>63</bold></td>
<td/>
</tr>
<tr>
<td valign="top" align="left">Mean AU 04</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>51</bold></td>
<td valign="top" align="center">&#x2013;0.02</td>
<td valign="top" align="center">0.26</td>
<td valign="top" align="center"><bold>0</bold>.<bold>44</bold></td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">0.24</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>34</bold></td>
<td valign="top" align="center">&#x2013;0.10</td>
<td valign="top" align="center">&#x2013;0.16</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 05</td>
<td valign="top" align="center">&#x2013;0.01</td>
<td valign="top" align="center">&#x2013;0.03</td>
<td valign="top" align="center">&#x2013;0.02</td>
<td valign="top" align="center">0.12</td>
<td valign="top" align="center">0.06</td>
<td valign="top" align="center">0.08</td>
<td valign="top" align="center">0.04</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.10</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 06</td>
<td valign="top" align="center"><bold>0</bold>.<bold>57</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>53</bold></td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>21</bold></td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>45</bold></td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>45</bold></td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center">0.08</td>
<td valign="top" align="center">0.02</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 07</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>54</bold></td>
<td valign="top" align="center">&#x2013;0.12</td>
<td valign="top" align="center">0.27</td>
<td valign="top" align="center"><bold>0</bold>.<bold>38</bold></td>
<td valign="top" align="center">0.13</td>
<td valign="top" align="center">0.16</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>33</bold></td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">&#x2013;0.13</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 12</td>
<td valign="top" align="center"><bold>0</bold>.<bold>71</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>49</bold></td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>33</bold></td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>53</bold></td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>46</bold></td>
<td valign="top" align="center">0.28</td>
<td valign="top" align="center">0.16</td>
<td valign="top" align="center">0.04</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 14</td>
<td valign="top" align="center">&#x2013;0.26</td>
<td valign="top" align="center">0.05</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.22</td>
<td valign="top" align="center">&#x2013;0.10</td>
<td valign="top" align="center">0.12</td>
<td valign="top" align="center">&#x2013;0.20</td>
<td valign="top" align="center">0.09</td>
<td valign="top" align="center">&#x2013;0.02</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 15</td>
<td valign="top" align="center">&#x2013;0.13</td>
<td valign="top" align="center">0.05</td>
<td valign="top" align="center"><bold>0</bold>.<bold>34</bold></td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.25</td>
<td valign="top" align="center">0.09</td>
<td valign="top" align="center">&#x2013;0.11</td>
<td valign="top" align="center">&#x2013;0.06</td>
<td valign="top" align="center">&#x2013;0.17</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 17</td>
<td valign="top" align="center">&#x2013;0.22</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.08</td>
<td valign="top" align="center"><bold>0</bold>.<bold>33</bold></td>
<td valign="top" align="center">0.09</td>
<td valign="top" align="center">0.27</td>
<td valign="top" align="center">&#x2013;0.02</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.02</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 23</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>44</bold></td>
<td valign="top" align="center">&#x2013;0.01</td>
<td valign="top" align="center">0.29</td>
<td valign="top" align="center"><bold>0</bold>.<bold>38</bold></td>
<td valign="top" align="center">&#x2013;0.09</td>
<td valign="top" align="center">0.22</td>
<td valign="top" align="center">&#x2013;0.29</td>
<td valign="top" align="center">&#x2013;0.15</td>
<td valign="top" align="center">&#x2013;0.20</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 24</td>
<td valign="top" align="center">&#x2013;0.15</td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">0.16</td>
<td valign="top" align="center">&#x2013;0.17</td>
<td valign="top" align="center">0.16</td>
<td valign="top" align="center">&#x2013;0.06</td>
<td valign="top" align="center">0.10</td>
<td valign="top" align="center">&#x2013;0.02</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 25</td>
<td valign="top" align="center"><bold>0</bold>.<bold>50</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>50</bold></td>
<td valign="top" align="center">&#x2013;0.10</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>35</bold></td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>43</bold></td>
<td valign="top" align="center">0.10</td>
<td valign="top" align="center">0.05</td>
<td valign="top" align="center">&#x2013;0.02</td>
</tr>
<tr>
<td valign="top" align="left">Mean AU 43</td>
<td valign="top" align="center">0.22</td>
<td valign="top" align="center">&#x2013;0.16</td>
<td valign="top" align="center">&#x2013;0.24</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>33</bold></td>
<td valign="top" align="center">&#x2013;0.03</td>
<td valign="top" align="center">&#x2013;0.14</td>
<td valign="top" align="center">0.29</td>
<td valign="top" align="center">0.06</td>
<td valign="top" align="center">0.24</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 04</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>49</bold></td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center"><bold>0</bold>.<bold>52</bold></td>
<td valign="top" align="center">0.14</td>
<td valign="top" align="center"><bold>0</bold>.<bold>30</bold></td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>39</bold></td>
<td valign="top" align="center">&#x2013;0.13</td>
<td valign="top" align="center">&#x2013;0.28</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 05</td>
<td valign="top" align="center">0.08</td>
<td valign="top" align="center">0.10</td>
<td valign="top" align="center">&#x2013;0.06</td>
<td valign="top" align="center">0.19</td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center">0.06</td>
<td valign="top" align="center">&#x2013;0.03</td>
<td valign="top" align="center">0.02</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 06</td>
<td valign="top" align="center"><bold>0</bold>.<bold>61</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>55</bold></td>
<td valign="top" align="center">&#x2013;0.21</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>43</bold></td>
<td valign="top" align="center">0.11</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>41</bold></td>
<td valign="top" align="center">0.24</td>
<td valign="top" align="center">0.13</td>
<td valign="top" align="center">0.05</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 07</td>
<td valign="top" align="center">&#x2013;0.27</td>
<td valign="top" align="center">&#x2013;0.02</td>
<td valign="top" align="center">0.19</td>
<td valign="top" align="center"><bold>0</bold>.<bold>34</bold></td>
<td valign="top" align="center">0.18</td>
<td valign="top" align="center">0.19</td>
<td valign="top" align="center">&#x2013;0.13</td>
<td valign="top" align="center">&#x2013;0.01</td>
<td valign="top" align="center">&#x2013;0.18</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 12</td>
<td valign="top" align="center"><bold>0</bold>.<bold>72</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>52</bold></td>
<td valign="top" align="center">&#x2013;0.29</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>43</bold></td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>32</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>39</bold></td>
<td valign="top" align="center">0.16</td>
<td valign="top" align="center">0.05</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 14</td>
<td valign="top" align="center">&#x2013;0.17</td>
<td valign="top" align="center">&#x2013;0.02</td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center">0.23</td>
<td valign="top" align="center">&#x2013;0.19</td>
<td valign="top" align="center">0.22</td>
<td valign="top" align="center">&#x2013;0.04</td>
<td valign="top" align="center">0.12</td>
<td valign="top" align="center">0.00</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 15</td>
<td valign="top" align="center">&#x2013;0.04</td>
<td valign="top" align="center">&#x2013;0.03</td>
<td valign="top" align="center">0.16</td>
<td valign="top" align="center">0.26</td>
<td valign="top" align="center">0.04</td>
<td valign="top" align="center"><bold>0</bold>.<bold>31</bold></td>
<td valign="top" align="center">0.05</td>
<td valign="top" align="center">0.12</td>
<td valign="top" align="center">0.05</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 17</td>
<td valign="top" align="center">&#x2013;0.15</td>
<td valign="top" align="center">&#x2013;0.02</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center"><bold>0</bold>.<bold>38</bold></td>
<td valign="top" align="center">&#x2013;0.03</td>
<td valign="top" align="center"><bold>0</bold>.<bold>39</bold></td>
<td valign="top" align="center">0.13</td>
<td valign="top" align="center">0.13</td>
<td valign="top" align="center">0.13</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 23</td>
<td valign="top" align="center">&#x2013;0.17</td>
<td valign="top" align="center">0.05</td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center"><bold>0</bold>.<bold>37</bold></td>
<td valign="top" align="center">&#x2013;0.23</td>
<td valign="top" align="center">0.28</td>
<td valign="top" align="center">&#x2013;0.05</td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">&#x2013;0.12</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 24</td>
<td valign="top" align="center">&#x2013;0.08</td>
<td valign="top" align="center">0.04</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.28</td>
<td valign="top" align="center">&#x2013;0.23</td>
<td valign="top" align="center">0.27</td>
<td valign="top" align="center">&#x2013;0.01</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center">&#x2013;0.08</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 25</td>
<td valign="top" align="center"><bold>0</bold>.<bold>53</bold></td>
<td valign="top" align="center"><bold>0</bold>.<bold>53</bold></td>
<td valign="top" align="center">&#x2013;0.07</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>34</bold></td>
<td valign="top" align="center">0.18</td>
<td valign="top" align="center"><bold>&#x2013;0</bold>.<bold>38</bold></td>
<td valign="top" align="center">0.11</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">&#x2013;0.04</td>
</tr>
<tr>
<td valign="top" align="left">Peak AU 43</td>
<td valign="top" align="center">0.27</td>
<td valign="top" align="center">&#x2013;0.21</td>
<td valign="top" align="center">&#x2013;0.04</td>
<td valign="top" align="center">&#x2013;0.11</td>
<td valign="top" align="center">&#x2013;0.21</td>
<td valign="top" align="center">0.11</td>
<td valign="top" align="center">0.19</td>
<td valign="top" align="center">&#x2013;0.08</td>
<td valign="top" align="center">0.03</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn><p>Correlations &#x003E; 0.30 are in bold. PI, purchase intention, AU04 = brow lowerer, AU05 = upper, lid raiser, AU06 = cheek raiser, AU07 = lid tightener, AU12 = lip corner pull, AU14 = dimpler, AU15 = lip corner depressor, AU17 = chin raiser, AU23 = lip tightener, AU24 = lip pressor, AU25 = lips part, AU43 = eyes closed. Ad like, Advertisement likeability; Brand like, Brand likeability change; Purchase intention, brand purchase intention change.</p></fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="S3.SS3">
<title>Semiparametric models of AU activity</title>
<p>We fitted separate semiparametric additive mixed models to account for non-linear relationships while controlling for gender and stimuli as fixed factors and the participants and stimulus group as random factors. In the first step, we calculated regression models exclusively based on self-report ratings to test for specific relationships proposed by the affect-transfer hypothesis (see <xref ref-type="table" rid="T2">Table 2</xref>). It is evident that advertisement likeability is strongly predicted by joy ratings, brand likeability change is only significantly predicted by advertisement likeability, and purchase intention change is strongly predicted by brand likeability. This pattern strongly supports a hierarchical influence of advertisement and brand effects as postulated by the affect-transfer hypothesis.</p>
<table-wrap position="float" id="T2">
<label>TABLE 2</label>
<caption><p>Prediction of ad likeability, brand likeability change, and brand purchase intention change based on relevant self-report ratings.</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" colspan="3" style="color:#ffffff;background-color: #7f8080;">Ad like</td>
<td valign="top" align="center" colspan="3" style="color:#ffffff;background-color: #7f8080;">Brand like</td>
<td valign="top" align="center" colspan="3" style="color:#ffffff;background-color: #7f8080;">Purchase intention</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
</tr>
<tr>
<td valign="top" align="left">Brand like</td>
<td/>
<td/>
<td/>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>3.39</bold></td>
<td valign="top" align="center"><bold>124.62</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
</tr>
<tr>
<td valign="top" align="left">Ad like</td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>1.58</bold></td>
<td valign="top" align="center"><bold>72.64</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
<td valign="top" align="center">1.58</td>
<td valign="top" align="center">2.23</td>
<td valign="top" align="center">0.072</td>
</tr>
<tr>
<td valign="top" align="left">Joy</td>
<td valign="top" align="center"><bold>5.26</bold></td>
<td valign="top" align="center"><bold>185.8</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">3.39</td>
<td valign="top" align="center">0.066</td>
<td valign="top" align="center"><bold>2.80</bold></td>
<td valign="top" align="center"><bold>2.38</bold></td>
<td valign="top" align="center"><bold>0.045</bold></td>
</tr>
<tr>
<td valign="top" align="left"><italic>R</italic><sup>2</sup><sub>adj</sub></td>
<td valign="top" align="center" colspan="3">0.445</td>
<td valign="top" align="center" colspan="3">0.141</td>
<td valign="top" align="center" colspan="3">0.262</td>
</tr>
<tr>
<td valign="top" align="left"><italic>AIC</italic></td>
<td valign="top" align="center" colspan="3">3,969</td>
<td valign="top" align="center" colspan="3">4,891</td>
<td valign="top" align="center" colspan="3">4,646</td>
</tr>
<tr>
<td valign="top" align="left"><italic>BIC</italic></td>
<td valign="top" align="center" colspan="3">4,351</td>
<td valign="top" align="center" colspan="3">5,285</td>
<td valign="top" align="center" colspan="3">5,051</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn><p>Significant coefficients are in bold. The models are controlled for gender and stimuli as fixed factors and participants and stimulus groups as random factors. Ad like, Advertisement likeability; Brand like, Brand likeability change; Purchase intention, brand purchase intention change.</p></fn>
</table-wrap-foot>
</table-wrap>
<p>Next, we fitted separate models to predict joy ratings (<xref ref-type="table" rid="T3">Table 3</xref>), advertisement likeability (<xref ref-type="table" rid="T4">Table 4</xref>), brand likeability change (<xref ref-type="table" rid="T5">Table 5</xref>), and purchase intention change (<xref ref-type="table" rid="T6">Table 6</xref>) based on mean and peak AU activity. In addition, we estimated the combined effect of AU and rating scales to determine the relative predictiveness of AFC versus self-report for the models that contain advertisement and brand effect self-report ratings.</p>
<table-wrap position="float" id="T3">
<label>TABLE 3</label>
<caption><p>Prediction of joy ratings based on mean and peak Action Unit (AU) activity.</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" colspan="6" style="color:#ffffff;background-color: #7f8080;">Joy</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" colspan="3" style="color:#ffffff;background-color: #7f8080;"><bold>AU mean</bold></td>
<td valign="top" align="center" colspan="3" style="color:#ffffff;background-color: #7f8080;"><bold>AU peak</bold></td>
</tr>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
</tr>
<tr>
<td valign="top" align="left">AU04</td>
<td valign="top" align="center">1.51</td>
<td valign="top" align="center">1.24</td>
<td valign="top" align="center">0.431</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.787</td>
</tr>
<tr>
<td valign="top" align="left">AU05</td>
<td valign="top" align="center">1.42</td>
<td valign="top" align="center">0.13</td>
<td valign="top" align="center">0.747</td>
<td valign="top" align="center">1.51</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.847</td>
</tr>
<tr>
<td valign="top" align="left">AU06</td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>9.39</bold></td>
<td valign="top" align="center"><bold>0.002</bold></td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>6.70</bold></td>
<td valign="top" align="center"><bold>0.010</bold></td>
</tr>
<tr>
<td valign="top" align="left">AU07</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.25</td>
<td valign="top" align="center">0.617</td>
<td valign="top" align="center">1.31</td>
<td valign="top" align="center">0.64</td>
<td valign="top" align="center">0.403</td>
</tr>
<tr>
<td valign="top" align="left">AU12</td>
<td valign="top" align="center"><bold>4.27</bold></td>
<td valign="top" align="left"><bold>15.09</bold></td>
<td valign="top" align="left"><bold>&#x003C;0.001</bold></td>
<td valign="top" align="left"><bold>1.21</bold></td>
<td valign="top" align="left"><bold>34.27</bold></td>
<td valign="top" align="left"><bold>&#x003C;0.001</bold></td>
</tr>
<tr>
<td valign="top" align="left">AU14</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.644</td>
<td valign="top" align="center">3.70</td>
<td valign="top" align="center">1.13</td>
<td valign="top" align="center">0.321</td>
</tr>
<tr>
<td valign="top" align="left">AU15</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.69</td>
<td valign="top" align="center">0.194</td>
<td valign="top" align="center">1.03</td>
<td valign="top" align="center">1.73</td>
<td valign="top" align="center">0.188</td>
</tr>
<tr>
<td valign="top" align="left">AU17</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">0.939</td>
<td valign="top" align="center">1.06</td>
<td valign="top" align="center">0.06</td>
<td valign="top" align="center">0.859</td>
</tr>
<tr>
<td valign="top" align="left">AU23</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.39</td>
<td valign="top" align="center">0.534</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.90</td>
<td valign="top" align="center">0.168</td>
</tr>
<tr>
<td valign="top" align="left">AU24</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.04</td>
<td valign="top" align="center">0.841</td>
<td valign="top" align="center">1.55</td>
<td valign="top" align="center">0.66</td>
<td valign="top" align="center">0.340</td>
</tr>
<tr>
<td valign="top" align="left">AU25</td>
<td valign="top" align="center">2.47</td>
<td valign="top" align="center">2.53</td>
<td valign="top" align="center">0.125</td>
<td valign="top" align="center">1.37</td>
<td valign="top" align="center">0.78</td>
<td valign="top" align="center">0.357</td>
</tr>
<tr>
<td valign="top" align="left">AU43</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.80</td>
<td valign="top" align="center">0.373</td>
<td valign="top" align="center">1.71</td>
<td valign="top" align="center">0.66</td>
<td valign="top" align="center">0.561</td>
</tr>
<tr>
<td valign="top" align="left"><italic>R</italic><sup>2</sup><italic><sub>adj</sub></italic></td>
<td valign="top" align="center" colspan="3">0.373</td>
<td valign="top" align="center" colspan="3">0.373</td>
</tr>
<tr>
<td valign="top" align="left"><italic>AIC</italic></td>
<td valign="top" align="center" colspan="3">4,249</td>
<td valign="top" align="center" colspan="3">4,244</td>
</tr>
<tr>
<td valign="top" align="left"><italic>BIC</italic></td>
<td valign="top" align="center" colspan="3">4,752</td>
<td valign="top" align="center" colspan="3">4,748</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn><p>Significant coefficients are in bold. The models are controlled for gender and stimuli as fixed factors and participants and stimulus groups as random factors. AU04 = brow lowerer, AU05 = upper, lid raiser, AU06 = cheek raiser, AU07 = lid tightener, AU12 = lip corner pull, AU14 = dimpler, AU15 = lip corner depressor, AU17 = chin raiser, AU23 = lip tightener, AU24 = lip pressor, AU25 = lips part, AU43 = eyes closed.</p></fn>
</table-wrap-foot>
</table-wrap>
<table-wrap position="float" id="T4">
<label>TABLE 4</label>
<caption><p>Prediction of advertisement likeability ratings based on mean and peak Action Unit (AU) activity with and without self-report ratings.</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" colspan="12" style="color:#ffffff;background-color: #7f8080;">Ad like ratings</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU mean</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU mean + ratings</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU peak</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU peak + ratings</bold></td>
</tr>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
</tr>
<tr>
<td valign="top" align="left">Joy</td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>5.27</bold></td>
<td valign="top" align="center"><bold>152.66</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>5.69</bold></td>
<td valign="top" align="center"><bold>135.39</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
</tr>
<tr>
<td valign="top" align="left">AU04</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">3.53</td>
<td valign="top" align="center">0.060</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.99</td>
<td valign="top" align="center">0.159</td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>6.87</bold></td>
<td valign="top" align="center"><bold>0.009</bold></td>
<td valign="top" align="center"><bold>1.06</bold></td>
<td valign="top" align="center"><bold>7.67</bold></td>
<td valign="top" align="center"><bold>0.005</bold></td>
</tr>
<tr>
<td valign="top" align="left">AU05</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">0.932</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.08</td>
<td valign="top" align="center">0.778</td>
<td valign="top" align="center">1.29</td>
<td valign="top" align="center">0.06</td>
<td valign="top" align="center">0.818</td>
<td valign="top" align="center">2.53</td>
<td valign="top" align="center">1.94</td>
<td valign="top" align="center">0.187</td>
</tr>
<tr>
<td valign="top" align="left">AU06</td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>13.95</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>4.46</bold></td>
<td valign="top" align="center"><bold>0.035</bold></td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>10.33</bold></td>
<td valign="top" align="center"><bold>0.001</bold></td>
<td valign="top" align="center">2.17</td>
<td valign="top" align="center">2.78</td>
<td valign="top" align="center">0.059</td>
</tr>
<tr>
<td valign="top" align="left">AU07</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.35</td>
<td valign="top" align="center">0.245</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">2.75</td>
<td valign="top" align="center">0.097</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.45</td>
<td valign="top" align="center">0.229</td>
<td valign="top" align="center">1.14</td>
<td valign="top" align="center">3.51</td>
<td valign="top" align="center">0.058</td>
</tr>
<tr>
<td valign="top" align="left">AU12</td>
<td valign="top" align="center"><bold>4.89</bold></td>
<td valign="top" align="left"><bold>17.96</bold></td>
<td valign="top" align="left"><bold>&#x003C;0.001</bold></td>
<td valign="top" align="center"><bold>1.59</bold></td>
<td valign="top" align="center"><bold>6.74</bold></td>
<td valign="top" align="center"><bold>0.002</bold></td>
<td valign="top" align="center"><bold>2.11</bold></td>
<td valign="top" align="left"><bold>29.22</bold></td>
<td valign="top" align="left"><bold>&#x003C;0.001</bold></td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>9.31</bold></td>
<td valign="top" align="center"><bold>0.002</bold></td>
</tr>
<tr>
<td valign="top" align="left">AU14</td>
<td valign="top" align="center">2.72</td>
<td valign="top" align="center">1.69</td>
<td valign="top" align="center">0.124</td>
<td valign="top" align="center"><bold>2.70</bold></td>
<td valign="top" align="center"><bold>3.00</bold></td>
<td valign="top" align="center"><bold>0.033</bold></td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center">0.679</td>
<td valign="top" align="center">1.11</td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center">0.757</td>
</tr>
<tr>
<td valign="top" align="left">AU15</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.954</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.74</td>
<td valign="top" align="center">0.188</td>
<td valign="top" align="center">1.32</td>
<td valign="top" align="center">0.34</td>
<td valign="top" align="center">0.509</td>
<td valign="top" align="center">1.72</td>
<td valign="top" align="center">0.43</td>
<td valign="top" align="center">0.671</td>
</tr>
<tr>
<td valign="top" align="left">AU17</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.13</td>
<td valign="top" align="center">0.722</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.20</td>
<td valign="top" align="center">0.655</td>
<td valign="top" align="center">1.51</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center">0.827</td>
<td valign="top" align="center">1.10</td>
<td valign="top" align="center">0.30</td>
<td valign="top" align="center">0.559</td>
</tr>
<tr>
<td valign="top" align="left">AU23</td>
<td valign="top" align="center">1.13</td>
<td valign="top" align="center">0.04</td>
<td valign="top" align="center">0.859</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.796</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.04</td>
<td valign="top" align="center">0.307</td>
<td valign="top" align="center">1.95</td>
<td valign="top" align="center">0.67</td>
<td valign="top" align="center">0.541</td>
</tr>
<tr>
<td valign="top" align="left">AU24</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.12</td>
<td valign="top" align="center">0.290</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.23</td>
<td valign="top" align="center">0.268</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.21</td>
<td valign="top" align="center">0.271</td>
<td valign="top" align="center">1.48</td>
<td valign="top" align="center">0.23</td>
<td valign="top" align="center">0.838</td>
</tr>
<tr>
<td valign="top" align="left">AU25</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.24</td>
<td valign="top" align="center">0.266</td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>4.98</bold></td>
<td valign="top" align="center"><bold>0.026</bold></td>
<td valign="top" align="center">1.48</td>
<td valign="top" align="center">2.03</td>
<td valign="top" align="center">0.234</td>
<td valign="top" align="center">1.81</td>
<td valign="top" align="center">2.04</td>
<td valign="top" align="center">0.191</td>
</tr>
<tr>
<td valign="top" align="left">AU43</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.968</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.14</td>
<td valign="top" align="center">0.704</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">0.926</td>
<td valign="top" align="center">1.72</td>
<td valign="top" align="center">0.41</td>
<td valign="top" align="center">0.634</td>
</tr>
<tr>
<td valign="top" align="left"><italic>R</italic><sup>2</sup><italic><sub>adj</sub></italic></td>
<td valign="top" align="center" colspan="3">0.250</td>
<td valign="top" align="center" colspan="3">0.455</td>
<td valign="top" align="center" colspan="3">0.247</td>
<td valign="top" align="center" colspan="3">0.457</td>
</tr>
<tr>
<td valign="top" align="left"><italic>AIC</italic></td>
<td valign="top" align="center" colspan="3">4,652</td>
<td valign="top" align="center" colspan="3">4,038</td>
<td valign="top" align="center" colspan="3">4,643</td>
<td valign="top" align="center" colspan="3">4,042</td>
</tr>
<tr>
<td valign="top" align="left"><italic>BIC</italic></td>
<td valign="top" align="center" colspan="3">5,155</td>
<td valign="top" align="center" colspan="3">4,552</td>
<td valign="top" align="center" colspan="3">5,147</td>
<td valign="top" align="center" colspan="3">4,556</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn><p>Significant coefficients are in bold. The models are controlled for gender and stimuli as fixed factors and participants and stimulus groups as random factors. AU04 = brow lowerer, AU05 = upper, lid raiser, AU06 = cheek raiser, AU07 = lid tightener, AU12 = lip corner pull, AU14 = dimpler, AU15 = lip corner depressor, AU17 = chin raiser, AU23 = lip tightener, AU24 = lip pressor, AU25 = lips part, AU43 = eyes closed. Ad like, Advertisement likeability.</p></fn>
</table-wrap-foot>
</table-wrap>
<table-wrap position="float" id="T5">
<label>TABLE 5</label>
<caption><p>Prediction of brand likeability change ratings based on mean and peak Action Unit (AU) activity with and without self-report ratings.</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" colspan="12" style="color:#ffffff;background-color: #7f8080;">Brand like change ratings</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU mean</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU mean + ratings</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU peak</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU peak + ratings</bold></td>
</tr>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
</tr>
<tr>
<td valign="top" align="left">Ad like</td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>1.59</bold></td>
<td valign="top" align="center"><bold>67.95</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>1.22</bold></td>
<td valign="top" align="center"><bold>82.76</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
</tr>
<tr>
<td valign="top" align="left">Joy</td>
<td/>
<td/>
<td/>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">3.11</td>
<td valign="top" align="center">0.078</td>
<td/>
<td/>
<td/>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">2.51</td>
<td valign="top" align="center">0.114</td>
</tr>
<tr>
<td valign="top" align="left">AU04</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.998</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.43</td>
<td valign="top" align="center">0.511</td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>10.78</bold></td>
<td valign="top" align="center"><bold>0.001</bold></td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>7.43</bold></td>
<td valign="top" align="center"><bold>0.006</bold></td>
</tr>
<tr>
<td valign="top" align="left">AU05</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.961</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">0.861</td>
<td valign="top" align="center">1.14</td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">0.958</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.793</td>
</tr>
<tr>
<td valign="top" align="left">AU06</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">0.938</td>
<td valign="top" align="center">1.41</td>
<td valign="top" align="center">0.68</td>
<td valign="top" align="center">0.360</td>
<td valign="top" align="center">2.09</td>
<td valign="top" align="center">1.91</td>
<td valign="top" align="center">0.135</td>
<td valign="top" align="center">2.11</td>
<td valign="top" align="center">2.11</td>
<td valign="top" align="center">0.101</td>
</tr>
<tr>
<td valign="top" align="left">AU07</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.794</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.04</td>
<td valign="top" align="center">0.833</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.89</td>
<td valign="top" align="center">0.347</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.36</td>
<td valign="top" align="center">0.550</td>
</tr>
<tr>
<td valign="top" align="left">AU12</td>
<td valign="top" align="center"><bold>1.90</bold></td>
<td valign="top" align="center"><bold>4.57</bold></td>
<td valign="top" align="center"><bold>0.007</bold></td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.39</td>
<td valign="top" align="center">0.240</td>
<td valign="top" align="center"><bold>2.31</bold></td>
<td valign="top" align="center"><bold>4.06</bold></td>
<td valign="top" align="center"><bold>0.012</bold></td>
<td valign="top" align="center">2.45</td>
<td valign="top" align="center">1.21</td>
<td valign="top" align="center">0.400</td>
</tr>
<tr>
<td valign="top" align="left">AU14</td>
<td valign="top" align="center">2.40</td>
<td valign="top" align="center">1.83</td>
<td valign="top" align="center">0.100</td>
<td valign="top" align="center">2.05</td>
<td valign="top" align="center">1.08</td>
<td valign="top" align="center">0.307</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.08</td>
<td valign="top" align="center">0.778</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.18</td>
<td valign="top" align="center">0.672</td>
</tr>
<tr>
<td valign="top" align="left">AU15</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.55</td>
<td valign="top" align="center">0.457</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.75</td>
<td valign="top" align="center">0.387</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.17</td>
<td valign="top" align="center">0.680</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.05</td>
<td valign="top" align="center">0.830</td>
</tr>
<tr>
<td valign="top" align="left">AU17</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.41</td>
<td valign="top" align="center">0.521</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.68</td>
<td valign="top" align="center">0.410</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.88</td>
<td valign="top" align="center">0.349</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.83</td>
<td valign="top" align="center">0.363</td>
</tr>
<tr>
<td valign="top" align="left">AU23</td>
<td valign="top" align="center">2.11</td>
<td valign="top" align="center">1.19</td>
<td valign="top" align="center">0.255</td>
<td valign="top" align="center">1.96</td>
<td valign="top" align="center">1.25</td>
<td valign="top" align="center">0.339</td>
<td valign="top" align="center">1.84</td>
<td valign="top" align="center">0.89</td>
<td valign="top" align="center">0.336</td>
<td valign="top" align="center">1.87</td>
<td valign="top" align="center">0.96</td>
<td valign="top" align="center">0.358</td>
</tr>
<tr>
<td valign="top" align="left">AU24</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.02</td>
<td valign="top" align="center">0.313</td>
<td valign="top" align="center">1.32</td>
<td valign="top" align="center">0.40</td>
<td valign="top" align="center">0.497</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.37</td>
<td valign="top" align="center">0.541</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.10</td>
<td valign="top" align="center">0.750</td>
</tr>
<tr>
<td valign="top" align="left">AU25</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.965</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center">0.701</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.08</td>
<td valign="top" align="center">0.773</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">0.917</td>
</tr>
<tr>
<td valign="top" align="left">AU43</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.02</td>
<td valign="top" align="center">0.882</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.995</td>
<td valign="top" align="center">1.14</td>
<td valign="top" align="center">0.26</td>
<td valign="top" align="center">0.733</td>
<td valign="top" align="center">1.34</td>
<td valign="top" align="center">0.35</td>
<td valign="top" align="center">0.756</td>
</tr>
<tr>
<td valign="top" align="left"><italic>R</italic><sup>2</sup><italic><sub>adj</sub></italic></td>
<td valign="top" align="center" colspan="3">0.057</td>
<td valign="top" align="center" colspan="3">0.142</td>
<td valign="top" align="center" colspan="3">0.064</td>
<td valign="top" align="center" colspan="3">0.146</td>
</tr>
<tr>
<td valign="top" align="left"><italic>AIC</italic></td>
<td valign="top" align="center" colspan="3">5,137</td>
<td valign="top" align="center" colspan="3">4,995</td>
<td valign="top" align="center" colspan="3">5,121</td>
<td valign="top" align="center" colspan="3">4,988</td>
</tr>
<tr>
<td valign="top" align="left"><italic>BIC</italic></td>
<td valign="top" align="center" colspan="3">5,640</td>
<td valign="top" align="center" colspan="3">5,520</td>
<td valign="top" align="center" colspan="3">5,625</td>
<td valign="top" align="center" colspan="3">5,513</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn><p>Significant coefficients are in bold. The models are controlled for gender and stimuli as fixed factors and participants and stimulus groups as random factors. AU04 = brow lowerer, AU05 = upper, lid raiser, AU06 = cheek raiser, AU07 = lid tightener, AU12 = lip corner pull, AU14 = dimpler, AU15 = lip corner depressor, AU17 = chin raiser, AU23 = lip tightener, AU24 = lip pressor, AU25 = lips part, AU43 = eyes closed. Ad like, Advertisement likeability; Brand like, Brand likeability change.</p></fn>
</table-wrap-foot>
</table-wrap>
<table-wrap position="float" id="T6">
<label>TABLE 6</label>
<caption><p>Prediction of brand purchase intention change rating based on mean and peak Action Unit (AU) activity with and without self-report ratings.</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" colspan="12" style="color:#ffffff;background-color: #7f8080;">Purchase intention</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU mean</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU mean + ratings</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU peak</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;" colspan="3"><bold>AU peak + ratings</bold></td>
</tr>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><bold>df</bold></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>F</bold></italic></td>
<td valign="top" align="center" style="color:#ffffff;background-color: #7f8080;"><italic><bold>p</bold></italic></td>
</tr>
<tr>
<td valign="top" align="left">Brand like</td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>4.18</bold></td>
<td valign="top" align="center"><bold>100.38</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>3.58</bold></td>
<td valign="top" align="center"><bold>117.77</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
</tr>
<tr>
<td valign="top" align="left">Ad like</td>
<td/>
<td/>
<td/>
<td valign="top" align="center">1.75</td>
<td valign="top" align="center">2.23</td>
<td valign="top" align="center">0.070</td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>1.71</bold></td>
<td valign="top" align="center"><bold>2.60</bold></td>
<td valign="top" align="center"><bold>0.049</bold></td>
</tr>
<tr>
<td valign="top" align="left">Joy</td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>2.79</bold></td>
<td valign="top" align="center"><bold>2.60</bold></td>
<td valign="top" align="center"><bold>0.035</bold></td>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>2.85</bold></td>
<td valign="top" align="center"><bold>2.69</bold></td>
<td valign="top" align="center"><bold>0.031</bold></td>
</tr>
<tr>
<td valign="top" align="left">AU04</td>
<td valign="top" align="center">2.36</td>
<td valign="top" align="center">2.27</td>
<td valign="top" align="center">0.205</td>
<td valign="top" align="center">2.04</td>
<td valign="top" align="center">2.22</td>
<td valign="top" align="center">0.128</td>
<td valign="top" align="center">1.39</td>
<td valign="top" align="center">1.09</td>
<td valign="top" align="center">0.228</td>
<td valign="top" align="center">1.34</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center">0.855</td>
</tr>
<tr>
<td valign="top" align="left">AU05</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.78</td>
<td valign="top" align="center">0.376</td>
<td valign="top" align="center">1.03</td>
<td valign="top" align="center">1.30</td>
<td valign="top" align="center">0.260</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.25</td>
<td valign="top" align="center">0.621</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.26</td>
<td valign="top" align="center">0.613</td>
</tr>
<tr>
<td valign="top" align="left">AU06</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.28</td>
<td valign="top" align="center">0.597</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.92</td>
<td valign="top" align="center">0.337</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center">0.701</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.998</td>
</tr>
<tr>
<td valign="top" align="left">AU07</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.97</td>
<td valign="top" align="center">0.325</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.81</td>
<td valign="top" align="center">0.368</td>
<td valign="top" align="center">1.39</td>
<td valign="top" align="center">0.49</td>
<td valign="top" align="center">0.689</td>
<td valign="top" align="center">1.40</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.816</td>
</tr>
<tr>
<td valign="top" align="left">AU12</td>
<td valign="top" align="center">1.43</td>
<td valign="top" align="center">0.56</td>
<td valign="top" align="center">0.375</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.55</td>
<td valign="top" align="center">0.459</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.71</td>
<td valign="top" align="center">0.399</td>
<td valign="top" align="center">1.59</td>
<td valign="top" align="center">1.48</td>
<td valign="top" align="center">0.206</td>
</tr>
<tr>
<td valign="top" align="left">AU14</td>
<td valign="top" align="center"><bold>2.63</bold></td>
<td valign="top" align="center"><bold>6.28</bold></td>
<td valign="top" align="center"><bold>&#x003C;0.001</bold></td>
<td valign="top" align="center"><bold>1.73</bold></td>
<td valign="top" align="center"><bold>8.49</bold></td>
<td valign="top" align="center"><bold>0.003</bold></td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.82</td>
<td valign="top" align="center">0.178</td>
<td valign="top" align="center">1.51</td>
<td valign="top" align="center">2.21</td>
<td valign="top" align="center">0.231</td>
</tr>
<tr>
<td valign="top" align="left">AU15</td>
<td valign="top" align="center">1.52</td>
<td valign="top" align="center">0.18</td>
<td valign="top" align="center">0.780</td>
<td valign="top" align="center">2.22</td>
<td valign="top" align="center">1.07</td>
<td valign="top" align="center">0.518</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.01</td>
<td valign="top" align="center">0.905</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.44</td>
<td valign="top" align="center">0.507</td>
</tr>
<tr>
<td valign="top" align="left">AU17</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">2.56</td>
<td valign="top" align="center">0.110</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.96</td>
<td valign="top" align="center">0.162</td>
<td valign="top" align="center">1.16</td>
<td valign="top" align="center">0.04</td>
<td valign="top" align="center">0.860</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.74</td>
<td valign="top" align="center">0.391</td>
</tr>
<tr>
<td valign="top" align="left">AU23</td>
<td valign="top" align="center">1.53</td>
<td valign="top" align="center">0.27</td>
<td valign="top" align="center">0.719</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.40</td>
<td valign="top" align="center">0.527</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.24</td>
<td valign="top" align="center">0.625</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.959</td>
</tr>
<tr>
<td valign="top" align="left">AU24</td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>4.39</bold></td>
<td valign="top" align="center"><bold>0.036</bold></td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">3.04</td>
<td valign="top" align="center">0.081</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.798</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.78</td>
<td valign="top" align="center">0.377</td>
</tr>
<tr>
<td valign="top" align="left">AU25</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">1.26</td>
<td valign="top" align="center">0.262</td>
<td valign="top" align="center">1.54</td>
<td valign="top" align="center">1.53</td>
<td valign="top" align="center">0.361</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.36</td>
<td valign="top" align="center">0.550</td>
<td valign="top" align="center">1.00</td>
<td valign="top" align="center">0.38</td>
<td valign="top" align="center">0.538</td>
</tr>
<tr>
<td valign="top" align="left">AU43</td>
<td valign="top" align="center">1.43</td>
<td valign="top" align="center">2.39</td>
<td valign="top" align="center">0.068</td>
<td valign="top" align="center"><bold>1.00</bold></td>
<td valign="top" align="center"><bold>4.88</bold></td>
<td valign="top" align="center"><bold>0.027</bold></td>
<td valign="top" align="center">1.69</td>
<td valign="top" align="center">2.04</td>
<td valign="top" align="center">0.257</td>
<td valign="top" align="center">1.48</td>
<td valign="top" align="center">1.78</td>
<td valign="top" align="center">0.298</td>
</tr>
<tr>
<td valign="top" align="left"><italic>R</italic><sup>2</sup><italic><sub>adj</sub></italic></td>
<td valign="top" align="center" colspan="3">0.055</td>
<td valign="top" align="center" colspan="3">0.275</td>
<td valign="top" align="center" colspan="3">0.034</td>
<td valign="top" align="center" colspan="3">0.263</td>
</tr>
<tr>
<td valign="top" align="left"><italic>AIC</italic></td>
<td valign="top" align="center" colspan="3">5,151</td>
<td valign="top" align="center" colspan="3">4,733</td>
<td valign="top" align="center" colspan="3">5,175</td>
<td valign="top" align="center" colspan="3">4,752</td>
</tr>
<tr>
<td valign="top" align="left"><italic>BIC</italic></td>
<td valign="top" align="center" colspan="3">5,654</td>
<td valign="top" align="center" colspan="3">5,269</td>
<td valign="top" align="center" colspan="3">5,678</td>
<td valign="top" align="center" colspan="3">5,288</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn><p>Significant coefficients are in bold. The models are controlled for gender and stimuli as fixed factors and participants and stimulus groups as random factors. AU04 = brow lowerer, AU05 = upper, lid raiser, AU06 = cheek raiser, AU07 = lid tightener, AU12 = lip corner pull, AU14 = dimpler, AU15 = lip corner depressor, AU17 = chin raiser, AU23 = lip tightener, AU24 = lip pressor, AU25 = lips part, AU43 = eyes closed. Ad like, Advertisement likeability; Brand like, Brand likeability change; Purchase intention, brand purchase intention change.</p></fn>
</table-wrap-foot>
</table-wrap>
<sec id="S3.SS3.SSS1">
<title>Prediction of joy ratings</title>
<p>Joy ratings were significantly predicted by mean and peak activities of AU6 and AU12 (see <xref ref-type="table" rid="T3">Table 3</xref>). Mean AU12 (<xref ref-type="fig" rid="F2">Figure 2</xref>, Panel 1A) showed a non-linear association with joy ratings, with the highest values for moderate AU intensities. In contrast, AU12 peak (<xref ref-type="fig" rid="F3">Figure 3</xref>, Panel 1A), AU6 mean (<xref ref-type="fig" rid="F2">Figure 2</xref>, Panel 2A), and AU6 peak (<xref ref-type="fig" rid="F3">Figure 3</xref>, Panel 2A) activity showed a linear and strictly monotonically increasing function with regard to joy ratings.</p>
<fig id="F2" position="float">
<label>FIGURE 2</label>
<caption><p>Fitted smooth main effects model for Action Unit means of AU12 [lip corner pull, <bold>(1A&#x2013;1D)</bold>], AU06 [cheek raiser, <bold>(2A&#x2013;2D)</bold>], and AU14 [dimpler, <bold>(3A&#x2013;3D)</bold>]. The graphs show the estimated marginal effects on joy ratings <bold>(1A&#x2013;3A)</bold>, advertisement likeability <bold>(1B&#x2013;3B)</bold>, brand likeability change <bold>(1C&#x2013;3C)</bold>, and purchase intention change <bold>(1D&#x2013;3D)</bold>. The effects are centered around zero. The shaded areas show 95% pointwise confidence intervals.</p></caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fnins-17-1125983-g002.tif"/>
</fig>
<fig id="F3" position="float">
<label>FIGURE 3</label>
<caption><p>Fitted smooth main effects model for Action Unit peaks of AU12 [lip corner pull, <bold>(1A&#x2013;1D)</bold>], AU06 [cheek raiser, <bold>(2A&#x2013;2D)</bold>], and AU04 [brow raiser, <bold>(3A&#x2013;3D)</bold>]. The graphs show the estimated marginal effects on joy ratings <bold>(A)</bold>, advertisement likeability <bold>(B)</bold>, brand likeability change <bold>(C)</bold>, and purchase intention change <bold>(D)</bold>. The effects are centered around zero. The shaded areas show 95% pointwise confidence intervals.</p></caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fnins-17-1125983-g003.tif"/>
</fig>
</sec>
<sec id="S3.SS3.SSS2">
<title>Prediction of advertisement likeability</title>
<p>Advertisement likeability ratings were significantly predicted by mean activity of AU6 and AU12 as well as peak activity of AU4, AU6, and AU12 (see <xref ref-type="table" rid="T4">Table 4</xref>). Mean AU12 (<xref ref-type="fig" rid="F2">Figure 2</xref>, Panel 1B) showed a non-linear association with advertisement likeability ratings, with the highest values for moderate AU intensities. In contrast, AU12 peak (<xref ref-type="fig" rid="F3">Figure 3</xref>, Panel 1B), AU6 mean (<xref ref-type="fig" rid="F2">Figure 2</xref>, Panel 2B), AU6 peak (<xref ref-type="fig" rid="F3">Figure 3</xref>, Panel 2B) showed a linear and strictly monotonically increasing function, whereas AU4 peak activity (<xref ref-type="fig" rid="F3">Figure 3</xref>, Panel 3B) showed a linear and strictly monotonically decreasing function with regard to advertisement ratings. These effects remained still significant for most AUs if joy ratings were included in the models.</p>
</sec>
<sec id="S3.SS3.SSS3">
<title>Prediction of brand likeability change</title>
<p>Brand likeability change ratings were significantly predicted by mean and peak activity of AU12 as well as peak activity of AU4 (see <xref ref-type="table" rid="T5">Table 5</xref>). AU12 mean (<xref ref-type="fig" rid="F2">Figure 2</xref>, Panel 1C) and AU12 peak (<xref ref-type="fig" rid="F3">Figure 3</xref>, Panel 1C) showed strictly monotonically increasing functions, whereas AU4 peak activity (<xref ref-type="fig" rid="F3">Figure 3</xref>, Panel 3C) showed a strictly monotonically decreasing function with regard to brand likeability change ratings. These effects for brand likeability change ratings remained only significant for peak AU4 activity if joy and advertisement likeability ratings are included in the models.</p>
</sec>
<sec id="S3.SS3.SSS4">
<title>Prediction of purchase intention change</title>
<p>Purchase intention change ratings were significantly predicted by mean activity of AU14 and AU 24 (see <xref ref-type="table" rid="T6">Table 6</xref>). AU14 mean (<xref ref-type="fig" rid="F2">Figure 2</xref>, Panel 3D) and AU24 mean activity showed strictly monotonically decreasing functions with regard to purchase intention change ratings. These effects for purchase intention change ratings remained only significant for mean AU14 activity if joy, advertisement likeability, and brand likeability change ratings are included in the model.</p>
</sec>
<sec id="S3.SS3.SSS5">
<title>Variation in model fit</title>
<p>Notably, there was a large variation in model fit (i.e., adjusted explained variance), which depends on the specific criterium and whether self-report rating scales are included in the model in addition to the facial expression parameters. For models including only AU parameters, we observed that the explanation of variance was strong for emotion ratings of joy, moderate to strong for advertisement likeability, and small for brand effects such as likeability and purchase intention change. If AU parameters and relevant rating scales are jointly used, we were able to improve model fit significantly and found a strong variance explanation for advertisement likeability mainly driven by joy ratings, a moderate variance explanation for brand likeability change mainly driven by advertisement likeability ratings, and a strong variance explanation for purchase intention change mainly driven by brand likeability change. Taken together, we demonstrated that AU parameters predicted relevant advertisement and brand criteria beyond self-report (see also <xref ref-type="table" rid="T7">Table 7</xref>).</p>
<table-wrap position="float" id="T7">
<label>TABLE 7</label>
<caption><p>Predictions of emotional processes according to a universalist (<xref ref-type="bibr" rid="B18">Cordaro et al., 2018</xref>), an appraisal-driven approach (<xref ref-type="bibr" rid="B68">Scherer et al., 2018</xref>), and a summary of observations in the present study for the relevant subset of Action Units (AU).</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;">AU</td>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;">AU description</td>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"><xref ref-type="bibr" rid="B18">Cordaro et al., 2018</xref></td>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;"><xref ref-type="bibr" rid="B68">Scherer et al., 2018</xref></td>
<td valign="top" align="left" style="color:#ffffff;background-color: #7f8080;">Present findings</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">AU4</td>
<td valign="top" align="left">Brow lowerer<break/></td>
<td valign="top" align="left">Anger, confusion, disgust, pain, shame, sadness, and contempt</td>
<td valign="top" align="left">Novelty, unpleasant, goal obstructive, and high coping potential</td>
<td valign="top" align="left">Lower ad like (peak),<break/> lower brand like (peak)</td>
</tr>
<tr>
<td valign="top" align="left">AU6</td>
<td valign="top" align="left">Cheek raiser<break/></td>
<td valign="top" align="left">Amusement, triumph, joy, desire, coyness, embarassment, disgust, and pain</td>
<td/>
<td valign="top" align="left">Higher joy (mean + peak),<break/> higher ad like (mean + peak)</td>
</tr>
<tr>
<td valign="top" align="left">AU12</td>
<td valign="top" align="left">Lip corner pull<break/></td>
<td valign="top" align="left">Amusement, triumph, joy, desire, coyness, embarassment, pride, content, relief, and awe</td>
<td valign="top" align="left">Pleasant,<break/> goal conductive</td>
<td valign="top" align="left">Higher joy (mean + peak),<break/> higher ad like (mean + peak),<break/> higher brand like (mean + peak)</td>
</tr>
<tr>
<td valign="top" align="left">AU14</td>
<td valign="top" align="left">Dimpler</td>
<td valign="top" align="left">Contempt</td>
<td/>
<td valign="top" align="left">Lower ad like (mean)<break/> lower purchase intention (mean)</td>
</tr>
<tr>
<td valign="top" align="left">AU24</td>
<td valign="top" align="left">Lip pressor<break/></td>
<td/>
<td valign="top" align="left">Unpleasant,<break/> high coping potential</td>
<td valign="top" align="left">Lower purchase intention (mean)</td>
</tr>
<tr>
<td valign="top" align="left">AU25</td>
<td valign="top" align="left">Lips part</td>
<td valign="top" align="left">Amusement, triumph, joy, coyness, relief, embarassment; disgust, pain, sympathy, contempt, fear, awe, surprise</td>
<td valign="top" align="left">Pleasant, unpleasant, goal conductive,<break/> high coping potential, low coping potential</td>
<td valign="top" align="left">Higher ad like (mean)<break/></td>
</tr>
<tr>
<td valign="top" align="left">AU43</td>
<td valign="top" align="left">Eyes closed</td>
<td valign="top" align="left">Content, relief; pain, sadness, contempt, and boredom</td>
<td valign="top" align="left">Unpleasant,<break/> high coping potential</td>
<td valign="top" align="left">Lower purchase intention (mean)</td>
</tr>
</tbody>
</table></table-wrap>
</sec>
</sec>
</sec>
<sec id="S4" sec-type="discussion">
<title>Discussion</title>
<p>Commercials are thought to elicit emotions, but it is difficult to quantify viewers&#x2019; emotional responses objectively and whether this has the intended impact on consumers. With novel technology, we automatically analyzed facial expressions in response to a broad array of video commercials and predicted self-reports of advertisement and brand effects. Taken together, parameters extracted by an automated facial coding technology significantly predicted all dimensions of self-report measures. Hence, automatic facial coding can contribute to a better understanding of advertisement effects in addition to self-report.</p>
<p>However, there was also a tremendous difference in model fit and, particularly, the strength of effects: facial expressions predicted emotion ratings with strong effects, advertisement likeability with moderate effects, changes in brand likeability, and purchase intention only with small effects. Furthermore, relations between self-report ratings strongly support a hierarchical influence of advertisement and brand effects as postulated by the affect-transfer-hypothesis: We found strong associations between joy and advertisement likeability, moderate effects between advertisement likeability and changes in brand likeability and again strong relations between brand likeability and change in purchase intention elicited by video commercials intention. Accordingly, AFC might be a valid indicator for measuring joy experience, advertisement, and brand effects, but the relevant self-report dimension still predicted the investigated criteria with more substantial effects.</p>
<p><xref ref-type="table" rid="T7">Table 7</xref> summarizes the specific effects of mean and peak AU activity on the investigated criteria. AU12 (lip corner pull) compared to other AU was a significant predictor of joy experience, ad likeability, and brand likeability change, which is in line with previous research (<xref ref-type="bibr" rid="B45">Lewinski et al., 2014b</xref>; <xref ref-type="bibr" rid="B50">McDuff et al., 2014</xref>, <xref ref-type="bibr" rid="B51">2015</xref>). In contrast to the previous report (<xref ref-type="bibr" rid="B79">Teixeira et al., 2014</xref>; <xref ref-type="bibr" rid="B51">McDuff et al., 2015</xref>), we observed no significant relationship between AU12 and purchase intention. However, we found facial activity related to unpleasant emotional states to predict purchase intention, such as AU14 (Dimpler), AU24 (Lip Pressor), and AU43 (Eyes Closed). Such contradicting results may be explained by different operationalization of the purchase intention across studies: While previous literature measured purchase intention only per post-advertisement measures (<xref ref-type="bibr" rid="B45">Lewinski et al., 2014b</xref>; <xref ref-type="bibr" rid="B50">McDuff et al., 2014</xref>, <xref ref-type="bibr" rid="B51">2015</xref>; <xref ref-type="bibr" rid="B79">Teixeira et al., 2014</xref>), in the present study this construct was measured by pre to post changes. This is preferable because post-advertisement brand ratings are highly confounded with the selected brand stimuli and cannot validly measure the effects of commercials. Hence, we found several Action Unit patterns that were not investigated before, expanding our knowledge of the relation between facial expressions and advertisement and brand effects.</p>
<p>The presented findings also contribute to a better understanding of the relationships of different statistical aggregation strategies of AFC parameters. In particular, the experience and memorization of emotional events is influenced or even biased by different aggregates of such a dynamic time-series (<xref ref-type="bibr" rid="B29">Fredrickson, 2000</xref>). Specifically, we analyzed mean and peak AU activities which are both widely used aggregates in emotion research. Importantly, we found coherence and exclusive contributions of peak and mean statistics. Mean and peak statistics of AU06 (cheek raiser) and AU12 (lip corner pull) show no meaningful differences in the prediction of joy ratings. However, predictions of advertisement likeability and brand likeability change also demonstrate a differential impact of specific AU patterns. For example, peak activity of AU04 (Brow Lowerer) had a significant effect on these criteria, which was not the case for mean values of AU04. Hence, our findings contribute to a better understanding of the differential impact of facial expression aggregates in advertisement and brand research. Future studies should investigate the role of other associated phenomena, such as the peak-end-bias (e.g., <xref ref-type="bibr" rid="B23">Do et al., 2008</xref>) and the stability of effects over time in advertisement research.</p>
<sec id="S4.SS1">
<title>Limitations and future directions</title>
<p>One aspect of the present study is the exclusive use of self-report ratings as the criteria in a cross-sectional design. The usage of <italic>ad hoc</italic> self-report scales has two significant limitations: First, it is unclear how stable reported advertisement effects on relevant brand dimensions are over time. Long-term effects of advertisement might be explored through longitudinal study design in future research, for example, by inviting participants again weeks or months after the main experiment to probe the stability of brand likeability and purchase intention changes. Second, it is unclear whether psychologically assessed intentions to purchase products or services of a particular brand elicit an actual purchase behavior. Future research should focus on predictions of actual behavior or even population-wide effects like it is approached with other methods in the consumer neuroscience literature (<xref ref-type="bibr" rid="B6">Berkman and Falk, 2013</xref>). Hence, out-of-sample criteria in the consumer research area that facial expression parameters might predict advertisement effects on a market-level response, such as monetary advertising elasticity estimates (<xref ref-type="bibr" rid="B87">Venkatraman et al., 2015</xref>) or video view frequencies on media platforms (<xref ref-type="bibr" rid="B82">Tong et al., 2020</xref>). Furthermore, facial responses toward music and movie trailers could predict actual sales figures in the music and movie industry, as already demonstrated with measures of neural response (<xref ref-type="bibr" rid="B7">Berns and Moore, 2012</xref>; <xref ref-type="bibr" rid="B8">Boksem and Smidts, 2015</xref>). Hence, future research needs to explore the predictive capability of facial expression recognition technology beyond within-subject measured self-report.</p>
<p>Automatic facial coding has also some advantages in comparison to emotional self-report because it enables a passive and non-contact assessment of emotional responses on a continuous basis. In contrast, emotional self-report is typically rated after stimulus presentation, and hence, reflect a more global and possibly biased evaluation of the recipients (e.g., <xref ref-type="bibr" rid="B53">M&#x00FC;ller et al., 2019</xref>). Furthermore, AFC provides a rich data stream of emotion-relevant facial movements, whereas self-report is typically assessed on a limited number of emotion scales. AFC technology enables a moment-to-moment analysis of elicited emotional responses, which allows for the assessment of emotional responses toward dynamic emotional content as in video commercials. For example, stories can have very different emotional dynamics such as an unpleasant beginning and a pleasant end and vice versa (<xref ref-type="bibr" rid="B61">Reagan et al., 2016</xref>). Hence, future research should investigate the differential impact of emotional dynamics of advertisement commercials and whether differences in the emotional dynamics affect relevant advertisement and brand effects.</p>
</sec>
</sec>
<sec id="S5" sec-type="conclusion">
<title>Conclusion</title>
<p>The present study identified facial expressions that were validated by self-reported emotional experience and predicted changes in brand likeability and purchase intention. Hence, this novel technology may be an excellent tool for tracking advertisement effects in real-time. Automatic facial coding enables a moment-to-moment analysis of emotional responses, non-invasive and non-contact. Accordingly, automatic emotional facial expression recognition is suitable for advertisement optimization based on emotional responses and for online research. Future research needs to evaluate the capability of such technology to predict actual consumer behavior beyond self-report and with out-of-sample criteria. Facial expressions can reveal very private emotional states and there will probably be a remarkable increase in the use of face recognition technology and its integration in everyday situations. Consequentially, many ethical issues will arise, specifically if applied in commercial and political contexts, and in particular if facial information is collected or analyzed without consent.</p>
</sec>
<sec id="S6" sec-type="data-availability">
<title>Data availability statement</title>
<p>The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: <ext-link ext-link-type="uri" xlink:href="https://madata.bib.uni-mannheim.de/id/eprint/410">https://madata.bib.uni-mannheim.de/id/eprint/410</ext-link>.</p>
</sec>
<sec id="S7" sec-type="ethics-statement">
<title>Ethics statement</title>
<p>The studies involving human participants were reviewed and approved by the Research Ethics Committee of the University of Mannheim. The patients/participants provided their written informed consent to participate in this study.</p>
</sec>
<sec id="S8" sec-type="author-contributions">
<title>Author contributions</title>
<p>TH contributed to the conception and design of the study, collected the data, performed the statistical analysis, and wrote the first draft of the manuscript. TH and GA reviewed and approved the final manuscript.</p>
</sec>
</body>
<back>
<sec id="S11" sec-type="funding-information">
<title>Funding</title>
<p>The publication of this manuscript was funded by the Ministry of Science, Research and the Arts Baden-W&#x00FC;rttemberg and the University of Mannheim.</p>
</sec>
<ack><p>We thank Ulrich F&#x00F6;hl and both reviewers for the valuable feedback on the manuscript.</p>
</ack>
<sec id="S12" sec-type="COI-statement">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec id="S13" sec-type="disclaimer">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<sec id="S14" sec-type="supplementary-material">
<title>Supplementary material</title>
<p>The Supplementary Material for this article can be found online at: <ext-link ext-link-type="uri" xlink:href="https://www.frontiersin.org/articles/10.3389/fnins.2023.1125983/full#supplementary-material">https://www.frontiersin.org/articles/10.3389/fnins.2023.1125983/full#supplementary-material</ext-link></p>
<supplementary-material xlink:href="Data_Sheet_1.pdf" id="DS1" mimetype="application/pdf" xmlns:xlink="http://www.w3.org/1999/xlink"/>
</sec>
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