The preoptic region (PO) was traditionally included within the hypothalamus until genoarchitectonic studies revealed that this region is derived from the FoxG1-positive telencephalic neuroephitelium (Tao and Lai, 1992; Murphy et al., 1994; Bourguignon et al., 1998; Zhao et al., 2009; Roth et al., 2010), revealing its subpallial nature (Flames et al., 2007; Medina, 2008; Garcia-Lopez et al., 2009; Moreno and González, 2011). In Xenopus, the PO is a Dll4, Isl1, Shh and Nkx2.1 positive territory that limits ventrally with the Otp expressing supraoptoparaventricular region (SPV, the dorsal part of the alar hypothalamus; Domínguez et al., 2013). This gene expression profile of the subpallial PO seems to be largely shared by reptiles (Pseudemys: Moreno et al., 2010) and the rest of amniotes (Puelles et al., 2000). However, some differences can be noted regarding its ventral boundary with the hypothalamus. In Xenopus, the Nkx2.1/Shh positive PO is in contact with the Otp/Nkx2.2 positive SPV (Figures 2A–D; Domínguez et al., 2013). In contrast, a narrow Nkx2.2 positive territory has been observed in the turtle (and not in Xenopus) between the Isl1/Nkx2.1 positive PO and the SPV-Otp expressing region (Figures 2E–H; Moreno et al., 2012). In this context, recent studies have described a Dlx/Nkx2.2 expressing band in mammals and birds that represents the boundary between the PO and the hypothalamus (Bardet et al., 2006, 2010; Flames et al., 2007), like in the turtle but in contrast to the situation found in amphibians and fishes (Domínguez et al., 2011, 2013; Moreno et al., 2012). Thus, the presence of this Nkx2.2 positive territory (preopto-hypothalamic boundary) supposes a relevant acquisition during the anamnio-amniotic transition.

The SPV is the most dorsal region in the alar hypothalamus and it is defined by the expression of Otp/Sim1 and the lack of Dlx/Shh/Nkx2.1 expression in all vertebrates analyzed (reviewed in Markakis, 2002; Medina, 2008; Moreno and González, 2011; Puelles et al., 2012a), from anamniotes (Brox et al., 2003; Del Giacco et al., 2006; Blechman et al., 2007; Bardet et al., 2008; Domínguez et al., 2010, 2013; Machluf et al., 2011; Martínez-de-la-Torre et al., 2011; Herget et al., 2014) to amniotes (Acampora et al., 1999; Flames et al., 2007; Bardet et al., 2008; Morales-Delgado et al., 2011, 2014).

Both in the amphibian Xenopus laevis and in the turtle Pseudemys scripta the extent of the SPV is particularly well defined by the expression of the transcription factor Otp, and its dorsal limit with the PO is defined by the lack of Isl1 expression (Figures 3A,G,I,N; Bardet et al., 2008; Moreno et al., 2012; Domínguez et al., 2013). The boundaries of the Otp positive SPV with the adjacent prethalamic and SC territories are also discernible by the lack of Isl1 in the SPV (Figures 3G,H,H’,N,O,O’; Moreno et al., 2012; Domínguez et al., 2013). The caudal boundary with the Tbr1-expressing prethalamic eminence (EPTh in P3) is also extremely conserved in the anamnio-amniotic transition (Figures 3G,N; Moreno et al., 2012; Domínguez et al., 2013). Thus, the lack of Dlx, Shh, Isl1, and Nkx2.1 in the SPV of all the vertebrates analyzed is a constant feature in tetrapods that allows the distinction of the SPV territory from the adjacent diencephalic prethalamus (PTh) and SC area (Flames et al., 2007; Medina, 2008; van den Akker et al., 2008; Domínguez et al., 2010, 2013; Moreno and González, 2011; Moreno et al., 2012; Puelles et al., 2012a; Morales-Delgado et al., 2014).

Interestingly, in some groups of fishes such as teleosts and lungfishes (the closest living relatives of tetrapods) the expression of Otp in the SPV territory has been reported (Del Giacco et al., 2006; Blechman et al., 2007; Machluf et al., 2011; Moreno and González, 2011). Moreover, a very recent study of the molecular and neurochemical features of this hypothalamic region in zebrafish identified a territory homologous to the mammalian paraventricular nucleus called neurosecretory preoptic-hypothalamic area (NPO), which is characterized by the expression of Otp and the lack of Isl1, Dlx5 and Arx, which define its anatomical boundaries (Herget et al., 2014).

Furthermore, both in Xenopus and Pseudemys the expression of the transcription factor Nkx2.2, allowed the rostro-caudal subdivision of the SPV into two different progenitor domains (Domínguez et al., 2011, 2013; Moreno et al., 2012). The rostral domain (SPVr) is characterized by the ventricular and subventricular expression of Otp and Nkx2.2, whereas the caudal domain (SPVc) only expresses Otp (Figures 3B,C,G,L,N; Moreno et al., 2012; Domínguez et al., 2013). In the case of Xenopus, Lhx5 has also been distinctly observed in the rostral domain of the SPV (Figure 3D; Domínguez et al., 2013), in agreement with descriptions of the SPV of mouse and chick (Bulfone et al., 1993; Abellán et al., 2010). Actually, attending to its internal molecular organization, this region in mammals and birds was divided rostrocaudally into terminal and peduncular portions (Bardet et al., 2008; Puelles et al., 2012a). In addition, regarding to the localization of the Nkx2.2, positive cells, they were reported partially overlapping the Sim1 expression domain in the anterior hypothalamus of the mouse, where the Nkx2.2 cell population seems to occupy a rostral position (see Figures 5K, 7D,E in Caqueret et al., 2006). And a population of Nkx2.2, expressing cells located in the ventral part of the paraventricular area in mouse has been described (Puelles et al., 2012a). In mammals, it was described that Nkx2.2 cells from the dorsal portion of the peduncular tuberal hypothalamus migrated very early colonizing this paraventricular nucleus, overlapping the Otp expressing cells (Puelles et al., 2012a).

The SPV of mammals and birds is also characterized by the expression of Pax6 and Tbr1 (Michaud et al., 1998, 2000; Puelles and Rubenstein, 2003; Flames et al., 2007; Medina, 2008). In the turtle, Pax6 expression was demonstrated in the ventricular zone of the SPV region (Figures 3J,K,N; Moreno et al., 2012), whereas it was not observed in Xenopus (Moreno et al., 2008a, 2012; Domínguez et al., 2013).The lack of Pax6 expression in the SPV has also been reported in other anamniotes (Murakami et al., 2001; Moreno and González, 2011). This situation might reflect differences in the specification of this area between amniotes and anamniotes. Therefore, the expression of Pax6 seems to have appeared for the first time in amniotes, and it could be related to the specific size and functionality of this area, being Pax6 involved in the dorsoventral brain organization, as it has been demonstrated in the mammalian forebrain (Toresson et al., 2000).

Of interest, some studies have recently described in amniotes a contingent of Otp positive cells generated in the SPV that migrate into the medial amygdala (Bardet et al., 2008; Abellán et al., 2010; García-Moreno et al., 2010; Bupesh et al., 2011a,b; Medina et al., 2011). This most likely represents a conserved feature that arose early in phylogeny since in lungfishes, anurans, and reptiles Otp expressing cells have been consistently observed in the region identified as the medial amygdala (González and Northcutt, 2009; Moreno et al., 2010; Domínguez et al., 2013), and a similar migratory pathway from the SPV was suggested (Moreno and González, 2011).

Regarding its neurochemical profile, the SPV of amphibians and reptiles contains different groups of cells secreting several neuropeptides such as vasotocine, mesotocine CRH, and TRH (Smeets et al., 1990; Propper et al., 1992; D’Aniello et al., 1999; Domínguez et al., 2008; López et al., 2008) that constitute part of the neuroendocrine hypothalamic system, which seems to be very conserved during the anamnio-amniotic transition. The TRH positive population in Xenopus and turtle, is specifically located within the Otp expressing territory (Domínguez et al., 2008; López et al., 2008), suggesting that this transcription factor might be involved in the specification of the TRH phenotype during the anamnio-amniotic transition, as describing in other amniotes (Goshu et al., 2004; Del Giacco et al., 2008; Morales-Delgado et al., 2014). Moreover, in Xenopus a correlation between the emergence of somatostatin and mesotocine positive neurons and the presence of Otp was observed in the SPV (Figures 3E,F; Domínguez et al., 2013), highlighting the role of this transcription factor in the specification of these postmitotic cell populations and, consequently, in the differentiation of independent nuclei within this territory. In mice it was shown that Otp is involved in the specification of the somatostatin expressing cell populations (Morales-Delgado et al., 2011) and, therefore, the specification of the somatostatin phenotype in the SPV seems to be conserved during the vertebrtae evolution. Of note, in fishes Otp might also be involved in the specification of multiple neurosecretory hypothalamic cell populations such as those containing somatostatin, vasoticin-neurophysin and isotocin-neurophysin (the latter two are homologous of mammalian vasopressin and oxytocin, respectively), as suggested by the overlapping of these neurosecretory populations in the Otp expressing domain (Blechman et al., 2007; Eaton and Glasgow, 2007; Tessmar-Raible et al., 2007; Eaton et al., 2008; Herget et al., 2014). Finally, a population of dopaminergic cells was described in the paraventricular nucleus of the SPV in reptiles (Figure 3M; Smeets et al., 1987), whereas catecholaminergic cells (TH positive) were not found in the SPV of Xenopus (Domínguez et al., 2013).

The SC constitutes the ventral part of the alar hypothalamus and contains important neuroendocrine cell groups. This region is characterized by the expression of Dlx/Arx genes in all vertebrates analyzed (Bachy et al., 2002; Brox et al., 2003; Puelles and Rubenstein, 2003; Flames et al., 2007; Bardet et al., 2008, 2010; Medina, 2008; Domínguez et al., 2011, 2013; Martínez-de-la-Torre et al., 2011). The SC abuts ventrally the basal hypothalamus, characterized by the expression of Shh/Nkx2.1 genes, defining the alar–basal boundary, according to the prosomeric model (Puelles et al., 2012a). Both in amphibians and reptiles, this ventral boundary is highlighted by the Otp expression in the most rostral part of the Tub (Figures 4G,H,L,M; Moreno et al., 2012; Domínguez et al., 2013). Caudally, in Xenopus, the SC region is adjacent to the Dll4 positive PTh in the diencephalon, whereas in the juvenile turtle the SPV Otp-expressing cells extends reaching the alar–basal boundary (Figures 4G,L; Moreno et al., 2012; Domínguez et al., 2013).

This region in mammals, identified as subparaventrcular area (Puelles et al., 2012a), is defined as a Dlx+/Nkx2.1-territory (Puelles and Rubenstein, 2003; Medina, 2008; Puelles et al., 2012a). Furthermore, a recent study in mice has defined a territory called intrahypothalamic diagonal band, based primarily on the differential expression of Lhx1, Lhx6, Lhx7 and Lhx8, constituting the territory from which the suprachiasmatic populations of interneurons would arise (Shimogori et al., 2010). The domain recently defined in mammals as the liminal subparaventricular subdomain distinctively contains alar mantle cells expressing Nkx2.1, not present at the supraliminal subdomain (Shimogori et al., 2010; Puelles et al., 2012a). It could be comparable to those results showed in our models, but in our case the Nkx2.1 expression is also detected in the ventricular cells.

The acroterminal domain in front of the terminal region was proposed to be the source of the proper suprachiacmaitc nucleus (Puelles et al., 2012a). Both in Xenopus and Pseudemys, no distinct labeling paterns could help in the deliniation of the acroterminal part of the alar hypothalamus but the specialized derivatives of this region have been classically described, such as the optic chiasm, postchiasmatic commissures and suprachiasmatic nuclei (for review, see ten Donkelaar, 1998a,b). Therefore, it is likely that the origin in the terminal region would be conserved, but further studies are needed in order to identify genoarchitectonically this territory.

In birds, Nkx2.1 is also expressed in the subparaventricular nucleus, that belongs to the suprachiasmatic domain, which also expresses Nkx2.2, Lhx6/7 and Lhx8 (Abellán and Medina, 2009; Bardet et al., 2010). This region of Xenopus and turtle is Isl1-positive (Figures 4A,G,I,L; Moreno et al., 2008b, 2012; Domínguez et al., 2013) and, in the case of Xenopus in which the expression of several Dlx genes has been analyzed, the Isl1 and Dlx expression domains overlap in almost all prosencephalic regions including the entire SC territory (Brox et al., 2003; Domínguez et al., 2010, 2013). The transcription factors Nkx2.1 and Nkx2.2, are also expressed in the SC (Figures 4B,G,J,L; Domínguez et al., 2010, 2011) and the combination of both markers allowed the identification of rostro-caudal subdivisions in Xenopus and Pseudemys. Thus, only in the rostral part (SCr) Nkx2.1 and Nkx2.2 are found in the ventricular zone, in contrast to the caudal portion (SCc) that is devoid of expression (Figures 4B,J; Moreno et al., 2012; Domínguez et al., 2013). Of note, in Xenopus, the expression pattern of the morphogen Shh in SC runs parallel to the Nkx2.1 expressing domain (Figures 4C,G), suggesting a regulatory role of Shh through Nkx2.1 actions also in amphibians (Domínguez et al., 2013). Also in Xenopus, the expression of Lhx1 and Lhx7 is restricted to the rostral SC domain (Figures 4D,E,G; Moreno et al., 2004; Domínguez et al., 2013), which would be comparable to the subparaventricular nucleus described in chicken (Abellán and Medina, 2009; Bardet et al., 2010). In the case of fishes, expression of Dlx and Lhx7 has been detected in comparable regions to the SC territory in Medaka (Alunni et al., 2004). In addition, expression of Dlx genes in the SC primordium has been observed in the lamprey (Martínez-de-la-Torre et al., 2011), and Isl1 and Nkx2.1 expressions have been detected in the SC region of lungfishes (Moreno and González, 2011). However, the region expressing Shh/Nkx2.1 in fishes extends to the entire SC territory and subdivisions were not observed (Rohr et al., 2001).

Regarding the Nkx2.1 expression in the alar hypothalamus, it appears that is gradually restricted especially in the SC, from amphibians through amniotes (Figure 7). In mammals, the Lhx6 + intrahypothalamic diagonal band, proposed by Shimogori et al. (2010) corresponds to the Nkx2.1 expressing band of Puelles in the subparaventricular region of the alar domain (see Figure 8.9D in Puelles et al., 2012a). Also in mammals, like in Xenopus and turtle, this small band matches the Nkx2.2 expression (see Figure 8.9F in Puelles et al., 2012a). It could be related to the liminal subparaventricular area proposed by Puelles et al. (2012a). Thus, Nkx2.1 and Shh are expressed in almost the SC territory in non-tetrapod anamniotes like the zebrafish (Rohr et al., 2001), whereas in non-mammalian tetrapods like the anamniote Xenopus and the amniote reptiles and birds the Nkx2.1 expression is restricted to just a SC subdomain (Medina, 2008; van den Akker et al., 2008; Abellán and Medina, 2009; Moreno et al., 2012; Domínguez et al., 2013). Consequently, the evolutionary tendency of the disappearance of both developmental regulators in the SC begins at the origin of the tetrapod phylogeny. Furthermore, this progressive disappearance of Shh/Nkx2.1 expression in the alar hypothalamus/SC region has been related to the pallial expansion that takes place in amniotes (Bruce and Neary, 1995; Striedter, 1997), and the reduction of the alar hypothalamus in amniotes in contrast to anamniotes at the expense of the thalamic expansion (van den Akker et al., 2008; for review, see Medina, 2008).

In functional terms, the SC region is known to belong to the neuroendocrine system and therefore consists of multiple neuropeptide-secreting cell populations. In mammals and birds, the SC is characterized by the presence of TRH positive cells, among others, that have also been reported in anamniotes such as anurans and fishes (Domínguez et al., 2008), but that were not detected in reptiles (López et al., 2008). However, the SC region in amphibians and reptiles is characterized by the presence of catecholaminergic cell groups (González and Smeets, 1991; González et al., 1993) restricted to the rostral domain (Figures 4F,G,K,L; Morona and González, 2008; Moreno et al., 2012; Domínguez et al., 2013). This feature appears to be conserved throughout vertebrate evolution, being observed in amniotes and anamniotes (Hökfelt et al., 1984; González et al., 1993; Smeets and González, 2000; Moreno et al., 2012; Domínguez et al., 2013). In addition, the GABAergic expression has been analyzed in the SC of Xenopus showing that it is widely distributed along the entire Dlx expressing zone (Domínguez et al., 2013), suggesting that Dlx could be involved in the GABAergic specification, like in mammals (Price et al., 1991; Bulfone et al., 1993; Marín and Rubenstein, 2001).

This region is currently considered to extend in the dorsal part of the basal hypothalamus, and like the rest of the hypothalamus has been postulated that posses acroterminal, terminal, and peduncular portions (Figure 1; Puelles et al., 2012a). The Tub is primarily characterized by the expression of Shh, which is directly involved in the organization of the basal hypothalamus through Nkx2.1 action (Kimura et al., 1996; Puelles et al., 2004), and thus Shh/Nkx2.1 expression has been observed in all the vertebrates analyzed (reviewed in Medina, 2008; Moreno and González, 2011). In amphibians and reptiles this territory is defined by the expression of Nkx2.1 and Isl1 (Figures 5A,B,F,H–J,M; Moreno et al., 2008b, 2012; Domínguez et al., 2014). Moreover, in Xenopus the Tub is also characterized by the ventricular expression of Shh, along with Nkx2.1 (Figures 5B,F; Domínguez et al., 2010, 2014), suggesting that also in Xenopus Shh could be implicated in the hypothalamic organization by the action of Nkx2.1, as in amniotes (Kimura et al., 1996; Puelles et al., 2004; reviewed in Medina, 2008). Consistently, both in Xenopus and Pseudemys the transcription factor Otp is exclusively located in the rostral tuberal portion (RT), within the Isl1-positive basal territory (Figures 5C,F,K–M).

However, in mammals the dorsal portion of the rostral terminal Tub is the only Otp expressing zone (Morales-Delgado et al., 2014), and the transcription factor Isl1 is expressed in the ventromedial and arcuate nuclei, where it is involved in the hypothalamic development and the regulation of the reproductive behavior (Davis et al., 2004). Moreover, the intermediate terminal and peduncular Tubs of mammals are also characterized by the expression of Dlx genes, involved in the specification of the GABAergic cell fate (Yee et al., 2009). Importantly, the transcription factor Otp is expressed in the dorsal Tub of mouse and in clusters of cells that from the acroterminal domain give rise to part of the nucleus arcuatus (Puelles et al., 2012a). Thus, the particular expression of Otp in a subdomain of the Tub appears as a conserved feature of the basal hypothalamus in amniotes and anamniotes equivalent to the region that gives rise to part of the arcuate nucleus in amniotes (Bardet et al., 2008; Puelles et al., 2012a).

Distinctly, the caudal tuberal part (CT) of Xenopus and Pseudemys is characterized by the lack of Otp expression (Figures 5C,F,K–M) and, expression of Nkx2.2 (Figures 5C–F), a transcription factor typically located in basal territories and necessary to maintain the ventral phenotype (Briscoe and Ericson, 1999; Sander et al., 2000; Garcia-Lopez et al., 2004; Puelles et al., 2004). Moreover, in mammals, Nkx2.2 in this Tub has been detected in the ventromedial nucleus and the core portion of the dorsomedial peduncular hypothalamic nucleus, in the terminal and peduncular domains respectively (Puelles et al., 2012a), where it is involved in the specification of the basal phenotype and the specification of the ventromedial fate (Kurrasch et al., 2007). Thus, it is possible that the Otp/Nkx2.1/Nkx2.2 expression in Xenopus likely resembles the mouse situation in which Otp is expressed in the dorsal domain of the terminal tuberal hypothalamus (likely including the acroterminal domain; Puelles et al., 2012a; Morales-Delgado et al., 2014), whereas Nkx2.1 is found in the terminal and peduncular intermediate portion (TuI), which gives rise to the ventromedial and dorsomedial nuclei (Puelles et al., 2012a), and likely corresponds to the region with Nkx2.2, Lhx1 and Dll4 expression found in our models, thus resembling the subdivision proposed (Figures 5D,F; Domínguez et al., 2014).

The boundary between the tuberal and mammillary territories in the basal hypothalamus of amphibians and reptiles is mainly defined by the lack of Isl1 expression in the mammillary region within the continuous Nkx2.1 positive tuberomammillar region (Figures 5F,G,M,N; Moreno et al., 2012; Domínguez et al., 2014). In addition, both regions can be distinguished by the differential expression of the transcription factor Otp, which is expressed in the mammillary region and not in the caudalmost tuberal domain (Figures 5C,F,L,M; Moreno et al., 2012; Domínguez et al., 2014). In Pseudemys a thin Nkx2.1 positive band (Moreno et al., 2012) can be detected between the Isl1 positive caudal tuberal zone and the Otp expressing mammillary region, defining specifically the tuberomammillary boundary (Moreno et al., 2012). In mammals, a thin ventral band expressing Dlx5/Nkx2.1/Arx+ but without Otp expression has been recently described in the ventral tuberal domain (Puelles et al., 2012a; Morales-Delgado et al., 2014). This is associated with a longitudinal circumventricular organ and it is the source of histamine in the hypothalamic cells (Puelles et al., 2012a).

Compared to tetrapods, there are only a few data about the hypothalamic organization in fishes, mainly attending to expression patterns and development. Recent studies in lunghfishes have revealed that Nkx2.1 and Isl1 are expressed in the entire Tub, whereas Otp expression is restricted to the most rostral and dorsal part, sustaining similar subdivisions in the tuberal territory to the ones described in tetrapods, using the same markers (Moreno and González, 2011). Data obtained in agnathans (lampreys) demonstrated that a substantial number of Dlx expressing cells occur in the tuberal hypothalamic nucleus and the tuberomammillary region, similar to anurans (Martínez-de-la-Torre et al., 2011).

The chemoarchitecture and neuronal specification processes in the Tub seem to be largely conserved throughout vertebrate evolution. In Xenopus, in the Otp-positive rostral Tub a population of somatostatin expressing cells has been observed (Figures 5E,F; Domínguez et al., 2014) suggesting the implication of Otp in the specification of this neurons in anurans, as has been previously reported in mammals (Acampora et al., 1999; Wang and Lufkin, 2000). In the mouse, the anterobasal nucleus, in the acroterminal domain defined by Puelles et al. (2012a), has been described to be the source of somatostatin cells to the ventromedial and arcuatus nucleus, where Otp would be specifically involved in the specification of these neurons (Morales-Delgado et al., 2011). The neuropeptide TRH, has been traditionally located in the dorsomedial nucleus (Hökfelt et al., 1975; Lechan et al., 1986; Tsuruo et al., 1987; Merchenthaler et al., 1988) and in the lateral hypothalamic area of mammals, where a recent study has proved its role in the arousal generation (Horjales-Araujo et al., 2014). However, a recent study has revealed that these tuberal TRH positive populations are likely generated in the SPV alar region (Morales-Delgado et al., 2014). The neuromodulator TRH has also been found in the periventricular hypothalamic nuclei of reptiles and in the Tub of anurans (Domínguez et al., 2008; López et al., 2008).

Finally, the anatomical position of the Dll4 expressing cell group located in the most caudal tuberal part of Xenopus is closely related to the GABAergic positive population (unpublished data), suggesting an implication of Dll4 in the specification of the GABAergic phenotype in the Tub, as occurs in the majority of the histogenetic domains where both markers colocalize (Price et al., 1991; Bulfone et al., 1993; Marín and Rubenstein, 2001). A distinct feature of the neurochemical profile of the Tub in birds is the presence of catecholaminergic populations originated under the control of Shh. Actually, in the chicken a dopaminergic positive population located in the Tub exists that is specified by Shh in a Six3-dependent manner (Ohyama et al., 2005). However, in anurans and reptiles there are not catecholaminergic cells in the Tub (Moreno et al., 2012; Domínguez et al., 2014).

In the current prosomeric model, the mammillary region is interpreted as formed by mammillary-terminal and retromammillary-peduncular regions (see Figure 1; Puelles et al., 2012a). In addition, immediately dorsal to them, corresponding peri-mammillary and peri-retromammillary regions (RMas) were considered. The latter form in mouse a rostral (peri-) band, which has been described based on the Otp/Nkx2.1 expression and the lack of Dlx genes (Puelles et al., 2012a).

In recent years, the regionalization of the this area has been under analysis and the terminology used for its various subdivisions and their actual extent in the basal hypothalamic region have progressively varied with the appearance of the molecular approach (Shimogori et al., 2010; Puelles et al., 2012a). By means of the combinatorial expression of Shh and Nkx2.1, in Xenopus two different regions were identified, the mammillary area proper (Ma) that is Otp/Nkx2.1+/Shh-, and the RMa, where the expression patterns of Shh and Nkx2.1 are inverted, being Nkx2.1-/Shh+ (Figures 6A,G; Domínguez et al., 2014). In contrast to Xenopus, in the turtle the Nkx2.1 expression is continuous throughout the mammillary band, abutting directly the Pax7 + p3b (Figures 6J,P; Moreno et al., 2012). However, in the turtle, but not in Xenopus (Figures 6B,C), within the Nkx2.1 expressing region (Figure 6O) a portion that is Isl1-/Otp- (Figure 6K, asterisk in K’) can be distinguished between the Isl1 + Tub and the Otp + and Nkx2.2 + Ma region (Figures 6K,L). It has been discussed (see above) that this region could correspond to the ventral tuberal portion proposed in mammals (Puelles et al., 2012a). In addition, the Ma of both reptiles and amphibians shows scattered Pax7 + cells in the subventricular zone late in development that likely originate in the Pax7 expressing population of the adjacent basal plate of P3 (Figures 6D,G–I,O,Q,Q’; Moreno et al., 2012; Domínguez et al., 2014), coincident with the Otp expression observed in the mammillary region (defined by the lack of Isl1 and the expression of Nkx2.1 and Otp; Moreno et al., 2012; Domínguez et al., 2014). This suggests that the longitudinally organization proposed in mammals, and specifically the rostral mammillary band, could also be present in Xenopus and turtle. In this line, in our models Nkx2.2 cells have been observed in the Ma Otp + zone (Figure 5L) and similarly Dlx and GABA expressing cells (Domínguez et al., 2014), likely from the adjacent p3b. In this context, it could also be possible that in mammals some of the Nkx2.2 expressing cells along the alar–basal boundary could reach the periretromammilar region, where Otp is observed in contrast to the perimammillar portion where only Otp is found (see Figure 8.26D in Puelles et al., 2012a).

The mammillary area is also characterized by the expression of genes of the LIM-HD family, whose combinatorial expression pattern led to propose a new regionalization of this territory in mammals (Shimogori et al., 2010). Thus, distinct nuclei were proposed including a supramammillary nucleus Irx5+, a premammillary nucleus expressing Lhx9/Lef1, a mammillary nucleus expressing Lhx1, and a tuberomammillary terminal zone positive for Lhx6, which is continuous with the diagonal band that separates the alar and basal hypothalamic regions (Shimogori et al., 2010). Comparatively, in Xenopus Lhx7 is expressed in the alar hypothalamus and continues ventrally into the mammillary territory (Moreno et al., 2004; Domínguez et al., 2013), and this led to propose the existence of a comparable tuberomammillary terminal zone in anurans (Domínguez et al., 2014). The expression of Lhx1 in the mammillary portion of the Xenopus hypothalamus was defined based on the lack of Isl1 in this portion and the Otp expression (Figure 5D; Domínguez et al., 2014). In addition, Lhx1 is also expressed in the mammillary portion of mouse hypothalamus (Bachy et al., 2002; Shimogori et al., 2010). Of note, in their hypothalamic analysis the group of Shimogori et al. (2010) used the Lhx1 expression to define the MM region (mammillary). However, in the current prosomeric interpretation (Puelles et al., 2012a) the expression of Lhx6 is interpreted in the ventral portion of the tuberal hypothalamus (see Figure 8.9 in Puelles et al., 2012a). Independently of its exact anatomical localization, its position in our models suggests, along with the Nkx2.2 and Pax7 expressing cells described before (Figures 5H,L,Q), a comparable longitudinal hypothalamic band that could be comparable to the rostral perimammillar and periretromammilar band (Puelles et al., 2012a).

Several studies in fishes have also reported the differential expression of LIM genes, such as Lhx6 and Lhx1/5, in the basal hypothalamic territory (Osorio et al., 2005; Menuet et al., 2007). Moreover, a recent study has analyzed the mammillary organization proposed by Shimogori et al. (2010) in zebrafish, finding different domains based on the differential expression of Lef1, Lhx6, Irx5 and Foxb1 (Wolf and Ryu, 2013). Thus, the presence of Otp in the Nkx2.1 positive region of the mammillary band was reported to be playing a crucial role in the establishment of different mammillary domains and is involved in the specification of posterior hypothalamic neurons regulating the expression of Fezf2 and Foxb1.2 in the putative mammillary region (Wolf and Ryu, 2013).

The lack of Shh expression in RMa has also been reported in the chicken, where the Shh becomes downregulated in the tuberomammillary primordium, but not in the RMa, at a specific point during development (Martí et al., 1995; Shimamura et al., 1995; Crossley et al., 2001; Patten et al., 2003; Manning et al., 2006), what seems to confer the hypothalamic fate to these cells (Manning et al., 2006). In the mammillary territory of mammals and birds, two rostro-caudal portions have been described on the basis of the differential expression of Nkx2.1 and Shh. Thus, there is a Nkx2.1+/Shh-region that is also positive for Otp (Bardet et al., 2008; Morales-Delgado et al., 2011), and a Nkx2.1-/Shh+ RMa region (García-Calero et al., 2008; Morales-Delgado et al., 2011). Attending to the Shh/Nkx2.1 expression pattern in the mammillary territory, it represents an exception within the prosencephalon, being the only forebrain area where Shh and Nkx2.1 are not expressed in parallel because Shh expression becomes secondarily downregulated at some point in the development (Shimamura et al., 1995; Crossley et al., 2001; Manning et al., 2006).

Thus, regarding the situation in fishes, some studies have described the presence of Shh in the basal hypothalamus, although so far there are no data about the specific location of Shh expression within this basal hypothalamic territory. It has been reported the presence of two hedgehog genes, expressed in a Sonic Hh-like pattern, in the basal hypothalamus of lamprey (Osorio et al., 2005; Kano et al., 2010). In addition, expression of Shh has been reported in the basal hypothalamus of cavefish and zebrafish (Menuet et al., 2007; Wolf and Ryu, 2013), where its expression seems to be limited in the tuberal territory, although no specific distinction of tuberal and mammillary regions was described.

In terms of chemical specification, the amphibian and reptilian mammillary region is characterized by the presence of a rich catecholaminergic cell population (Smeets et al., 1987; Smeets and González, 2000; Moreno et al., 2012; Domínguez et al., 2014) that co-expresses Nkx2.1 (Figures 6E,G,M,O) and Otp (Figures 6F,G,N,O), in line with previous studies in other vertebrates and suggesting a conserved role of both transcription factors in the specification of the dopaminergic phenotype during the anamnio-amniotic transition (Kawano et al., 2003; Del Giacco et al., 2006; Blechman et al., 2007; Ryu et al., 2007; Löhr et al., 2009).

Regarding the nuclear specification, controversy exists regarding the origin of the different neuronal groups and several data support the contribution of diencephalic areas to the mammillary territory. In mammals, the retromammillary area was considered a caudoventral hypothalamic specification located between the diencephalic tegmentum (in P3; see for review Puelles et al., 2012a), giving rise to the subthalamic nucleus. In birds, recent fate map studies have described that the basal plate of P3 generates the retromammillary tegmentum and the subthalamic nucleus (Garcia-Lopez et al., 2009). In reptiles and anurans, Pax7 expressing cells likely originated in P3 colonize the mammillary region (Moreno et al., 2012; Bandín et al., 2013; Domínguez et al., 2014), suggesting a diencephalic contribution to the formation of the hypothalamic mammillary territory. Moreover, the subthalamic nucleus in mammals was identified by the expression of Pax7 (Stoykova and Gruss, 1994; see mouse developmental Allen Brain Atlas), thus the Pax7 positive cells found dispersed in the mammilary territory in Xenopus could suggest the existence of a forerunner of the subthalamic nucleus in anurans (Domínguez et al., 2014).