AUTHOR=Li Zhiqiang , Kuang Xiaoyuan , Shan Ge , Wu Jiahong 

TITLE=Repeated Aedes albopictus bites reshape gut microbiota and repattern inflammatory readouts in a murine colitis model

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1702365

DOI=10.3389/fmicb.2025.1702365

ISSN=1664-302X

ABSTRACT=The gut microbiota represents a stable and dynamic symbiotic community that maintains host health and shapes immune homeostasis. Environmental exposures can disturb this symbiosis, yet the impact of repeated vector biting on host microbial communities has not been explored. Here, we investigated how repeated Aedes albopictus mosquito bites influence gut microbiota composition and stability in a murine model of dextran sulfate sodium (DSS)–induced colitis. Mice were repeatedly exposed to mosquito bites over several weeks prior to DSS treatment, and fecal microbiota were profiled using 16S rRNA sequencing at baseline, during inflammation (day 7), and recovery (day 14). Mosquito biting acted as a “press disturbance,” increasing microbial richness and community dispersion at baseline compared to unbitten controls. During DSS challenge, mosquito-exposed mice exhibited distinct microbial trajectories relative to DSS-only mice, including altered relative abundance of taxa such as Lactobacillus. These microbiota shifts were associated with changes in host inflammatory readouts, including elevated IL-6 during induction and partial normalization by day 14, as well as modest hematological adjustments. Our findings demonstrate that repeated vector exposure can reshape the gut microbiota, modulating the stability and composition of this core host symbiosis under inflammatory stress. These results highlight the sensitivity of symbiotic microbial communities to ecological perturbations and suggest that vector–host interactions may represent an underappreciated factor influencing host–microbe partnerships.