Front. Microbiol. Frontiers in Microbiology Front. Microbiol. 1664-302X Frontiers Media S.A. 10.3389/fmicb.2025.1630828 Microbiology Review Oral microbiome contributions to metabolic syndrome pathogenesis Yue Zijun 1 Fan Yue 1 Shan Guangliang 2 3 4 Chen Xingming 1 * 1Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China 2Department of Epidemiology and Statistics, School of Basic Medicine Peking, Union Medical College, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, Beijing, China 3State Key Laboratory of Common Mechanism Research for Major Diseases, Beijing, China 4School of Population Medicine and Public Health, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

Edited by: Shanshan Hu, Anhui Agricultural University, China

Reviewed by: Muhammad Hassan Sarfraz, Government College University, Pakistan

Ameer Luqman, Chongqing University, China

Xingmiao Lu, Tianjin University of Science and Technology, China

 *Correspondence: Xingming Chen xingming.chen@hotmail.com 08 08 2025 2025 16 1630828 18 05 2025 21 07 2025 Copyright © 2025 Yue, Fan, Shan and Chen. 2025 Yue, Fan, Shan and Chen

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Comprising over 700 bacterial species, the oral microbiome is the second most diverse microbial community in the human body after the gut microbiome. Currently, existing review literature suggests that gut microbiome events may play a significant role in the pathogenesis of metabolic syndrome, but the role of the oral microbiome in this disease has not yet been reviewed. The oral-gut microbiome axis refers to a bidirectional regulatory system that facilitates interaction between the oral cavity and the gut through microbial pathways. The microbiota from these two sites can migrate between each other via pathways such as swallowing and blood circulation, which may participate in disease development. In addition to the oral-gut axis, the oral microbiome itself may also influence disease pathogenesis. This review examines the potential contributions of the oral microbiome in the pathogenesis of metabolic syndrome, emphasizing its impact on insulin resistance, systemic inflammation and adipokine secretion. We explore therapeutic strategies targeting the oral microbiome which hold promise as future treatments for metabolic syndrome. Future research is needed to further elucidate the causal relationship between the oral microbiome and metabolic syndrome and to develop personalized microbiome-based therapies.

 microbiology oral microbiome metabolic syndrome oral pathogen microorganisms section-at-acceptance Microorganisms in Vertebrate Digestive Systems 1 Introduction

Insulin resistance, atherogenic dyslipidemia, central obesity, and hypertension are among the metabolic dysregulations that make up the metabolic syndrome (MetS; Fahed et al., 2022). It is a group of risk factors that frequently lead to increased metabolic abnormalities such as cardiovascular diseases (CVDs) and type 2 diabetic mellitus (T2DM) if left untreated (Dabke et al., 2019). MetS has caused a significant disease burden, with one-third of adult Americans suffering from MetS (Saklayen, 2018).

These days, numerous research have discovered a link between the elements of MetS and the oral microbiome, and the pathogenic mechanisms through which oral microbiome may contribute to the disease have been partially explored (Schamarek et al., 2023; Wang et al., 2023; Sanz et al., 2020; Chen et al., 2023; Mikami et al., 2021). However, a small number of studies did not find significant associations or causal relationships between the two (Kim et al., 2021; Yan et al., 2024). Nevertheless, despite these conflicting findings, a comprehensive review of the relationship between the oral microbiome and MetS remains necessary.

Comprising bacteria, microeukaryotes, viruses, and archaea, the human oral microbiome harbors a wide variety of over 700 bacterial species, making it the second most prevalent microbial community in the human body after the gut microbiome (Costa et al., 2024). The oral microbiome is possessed of better accessibility (Baker et al., 2024) and diversified ecological niches (Lu et al., 2022), including hard palate, soft palate, dental plaque, buccal mucosa, tongue dorsum, throat, and etc. (Figure 1). It may be more suitable for large-scale and repeated sampling to study the dynamic changes of the microbiome. Interventions related to the oral microbiome (Brookes et al., 2023; Bescos et al., 2020), such as oral hygiene practices (e.g., brushing, mouthwash), may be simpler and easier to implement.

Sampling sites for oral microbiome. Created in BioRender. Yue, Z. (2025) https://BioRender.com/ngzmkfw.

Illustration of an open mouth labeled with anatomical features: hard palate, buccal mucosa, tongue dorsum, dental plaque, soft palate, and throat. An inset shows a magnified view of dental plaque containing bacteria. A scale bar indicates centimeter.

The concept of oral-gut microbiome axis refers to the bidirectional interaction between oral and gut microbiome, encompassing microbial translocation, metabolite exchange, immune signaling, and systemic health impacts (Kitamoto et al., 2020b). Oral microbes can migrate to the gut through swallowing, hematogenous spread, or mucosal transfer, thereby altering gut microbiome composition and function, which subsequently influences host metabolism, immune responses, and disease development (Kitamoto et al., 2020a; Kunath et al., 2024).

Dysbiosis of the gut microbiome has already been found to be a key factor in the pathophysiology of MetS (Dabke et al., 2019). However, no literature has yet reviewed the role of the oral microbiome in the pathogenesis of MetS.

This review attempts to clarify the possible role of the oral microbiome in the pathogenesis of MetS from a mechanistic perspective. This may provide insights for future applications of oral microbiome biomarkers in MetS risk screening. We focus mainly on oral microbiome's influence on insulin resistance, chronic inflammation, and adipokine metabolism. To note, the concept of “central inflammation” mainly refers to the hematopoietic stem and progenitor cells (HSPCs) in the bone marrow serving as a central hub to regulate the host's adaptive response to inflammation (Chavakis et al., 2019). Finally, we give a brief overview of the oral microbiome-targeted management of MetS, offering perspectives and suggestions for further investigation.

 2 The role of oral microbiome in the pathogenesis of MetS

There are numerous intricate processes involved in the pathophysiology of MetS that have not yet been completely understood. Some studies hold the opinion that the onset of MetS occurs in the context of environmental, genetic, and dietary factors, leading to the accumulation of visceral adiposity (Fahed et al., 2022). This process results in insulin resistance, systemic inflammation, and alterations in adipokines secretion, which manifest as the phenotypes of MetS, such as obesity, dyslipidemia, and hypertension. Ultimately, these conditions contribute to an increased risk of CVDs (Fahed et al., 2022). MetS, historically termed insulin resistance syndrome, is fundamentally characterized by insulin resistance—a central phenotype that will be extensively discussed throughout this manuscript. Growing evidence demonstrates that insulin resistance interacts intricately with other key components in the pathogenesis of MetS (Lemieux and Després, 2020). Given the intersection of these mechanisms and phenotypes, this review highlights a key common molecule—free fatty acids (FFAs). FFAs are not only linked to insulin resistance, forming a vicious cycle, regulated by inflammatory factors, but also associated with adipose tissue metabolism. Therefore, the role of FFAs is mentioned in many parts of this review. This is not redundant but rather reflects the interconnected nature of MetS as a cluster of mutually influencing and interrelated conditions.

In addition to local oral diseases, it is becoming more well-acknowledged that the oral microbiome plays a significant part in the emergence of numerous systemic illnesses. Specifically, a number of extra-oral diseases, such as Alzheimer's disease, colorectal cancers, inflammatory bowel disease (IBD), rheumatoid arthritis, diabetes, obesity, and CVDs, have been linked to periodontal disease and its associated pathogens, such as Porphyromonas gingivalis (P. gingivalis), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), and Fusobacterium nucleatum (F. nucleatum). Some of these conditions are metabolic events linked to MetS (Kapila, 2021; Pirih et al., 2021; Jungbauer et al., 2022; Huang et al., 2023; Tonelli et al., 2023; Shaalan et al., 2022).

Direct pathogenic effects from local colonization of oral bacteria and a variety of indirect disease-promoting effects mediated by oral dysbiosis are the two primary, and sometimes complementary, ways by which the oral microbiome affects the pathophysiology of distal disorders (Baker et al., 2024; Imai et al., 2021).

2.1 The oral microbiome promotes insulin resistance through multiple mechanisms

Insulin resistance represents not merely a characteristic phenotypic manifestation of MetS, but rather one of its core pathogenic mechanisms. Specifically, in the context of visceral adiposity expansion, a vicious cycle forms between insulin resistance and increased plasma FFAs. Excess FFAs act on insulin downstream signaling pathways in muscle tissue, liver, and pancreas, leading to sustained high plasma glucose levels and hyperinsulinemia, thereby inducing insulin resistance (Figure 2). In turn, insulin resistance exacerbates the condition by impairing insulin's antilipolytic effects, resulting in increased plasma FFAs (Sun et al., 2021; Yao et al., 2022; Muniyappa, 2024). This vicious cycle contributes to systemic inflammation, dyslipidemia, obesity, and hypertension, with bidirectional interactions amplifying these effects (Fahed et al., 2022).

The oral microbiome influences systemic inflammation, insulin resistance, and hypertension through the oral-gut axis via various microbial metabolites and specific bacterial taxa. Upper portion of the figure: The illustration depicts how oral microbiome-derived immune cells and oral pathogens affect the gut via the oral-gut axis, shifting it from a healthy state to a dysbiotic, pro-inflammatory condition. This process disrupts gut barrier integrity, mediating systemic inflammation. Lower portion of the figure: The diagram highlights the role of oral microbial metabolites and specialized nitrate-reducing bacteria in modulating insulin resistance and hypertension through key signaling pathways, FFAs, and NO levels. Additionally, crosstalk between systemic inflammation and insulin resistance is illustrated. Visual guide: Black arrows: Represent progressive relationships; Red arrows: Indicate changes in metabolites; Blue arrows: Show pathway modifications; Blue background: Highlights MetS mechanisms and phenotypes discussed in this study; Pink background: Indicates microbiome-related metabolites; Yellow background: Marks relevant signaling pathways. Abbreviations: FFAs, free fatty acids; CCR9, CC-chemokine receptor 9; SCFAs, short-chain fatty acids; BCAAs, branched-chain amino acids; mTOR, mammalian target of rapamycin; S6K1, ribosomal protein S6 kinase1; IRS-1, insulin receptor substrate 1; NO, nitric oxide. Created in BioRender. Yue, Z. (2025) https://BioRender.com/0e0vmmc.

Diagram illustrating the connection between the oral microbiome and MetS phenotypes. Oral pathogens interact with T cells, influencing the oral-gut axis. This can lead to inflammation, compromising the gut barrier and causing systemic inflammation. Metabolites include SCFAs increasing FFAs, BCAAs affecting mTOR/S6K1/IRS-1 pathways contribute to insulin resistance. Reduced nitrate-reducing bacteria lead to lowered NO levels, thus promoting hypertension. In the end, systematic inflammation can mediate insulin resistance and the latter worsens hypertension. 2.1.1 Oral microbiome is associated with insulin resistance

One well-known instance of oral microbiome dysbiosis is periodontal disease. Patients with severe periodontitis had significantly higher homeostasis model assessment of insulin resistance (HOMA-IR) readings than the control group in a cross-sectional study of 74 participants in India. A strong correlation between periodontitis and insulin resistance is also suggested by the greater prevalence of prediabetes, incident diabetes, and insulin resistance in the group with severe periodontitis (George et al., 2021). Other cross-sectional studies have reached similar conclusions (Pulido-Moran et al., 2017; Kalhan et al., 2025). Furthermore, compared to standard care, local periodontal therapy has been shown to lower hemoglobin A1c (HbA1c) levels in prediabetes and T2DM patients (Greggianin et al., 2023). These findings collectively suggest a precise link between oral microbiome dysbiosis and insulin resistance.

 2.1.2 Oral pathogens may be associated with insulin resistance through their metabolites

Through metabolic processes, the oral microbiome generates and modifies a range of fatty acids (FAs), such as short-chain fatty acids (SCFAs). These metabolites have been proven to influence the local oral environment (e.g., pH and microbial balance; Magrin et al., 2020). In an animal study, Wu et al. (2018) found that inoculation with P. gingivalis increased total FFAs levels in the tongue tissue and plasma of mice and altered their plasma FFAs profiles. These changes may be linked to the upregulation of de novo FAs synthesis pathways in the tongue tissue by P. gingivalis. However, there lacks definitive human studies proving that these metabolites could enter the systemic circulation and regulate FFAs levels (Figure 2). It is believed that elevated levels of plasma FFAs have a role in the development of insulin resistance, consistent with observations in human studies showing increased FFAs in T2DM patients (Weijers, 2022). Given the existing evidence, we can only suspect that metabolites from oral microbiome may indirectly exacerbate insulin resistance by influencing FFAs metabolism, potentially promoting the development of MetS. Further studies are required to determine whether and how oral microbiome-derived metabolites influence blood FFAs levels, which will help elucidate the role of oral microbial lipid metabolism in systemic diseases.

 2.1.3 Insulin resistance caused by high-fat diet (HFD) is enhanced by the oral microbiome

One popular model for MetS is the HFD mouse. Oral gavage of three human periodontal pathogens [Prevotella intermedia (P. intermedia), F. nucleatum, and P. gingivalis] not only caused local inflammatory bone loss in mice given HFD, but it also seemed to exacerbate insulin resistance and glucose intolerance brought on by the diet. The ability to generate particular antibodies against lipopolysaccharide (LPS) from P. gingivalis is linked to the underlying mechanism (Blasco-Baque et al., 2017; Figure 2). The dietary intervention used in this study may mediate insulin resistance through accumulation of visceral fat, which increase plasma FFAs levels, consistent with the central idea mentioned above, that MetS begins with the expansion of visceral adiposity (Fahed et al., 2022).

Lu et al. (2023) reported similar findings, demonstrating that mice fed a HFD for 15 weeks and inoculated with P. gingivalis exhibited enhanced HFD-induced insulin secretion and insulin resistance compared to those on a low-fat diet (LFD; key parameters including insulin levels and HOMA-IR). However, in this study, the levels of FFAs in HFD-fed mice did not significantly increase compared to LFD-fed mice, suggesting that the mechanisms underlying insulin resistance in MetS are not limited to the vicious cycle driven by elevated plasma FFAs (Fahed et al., 2022). Insulin resistance may also occur as a result of additional mechanisms.

Through the synthesis of branched-chain amino acids (BCAAs), the oral microbiome may worsen insulin resistance brought on by HFD. The wild-type organism, but not a BCAAs aminotransferase-deficient mutant, caused elevated serum levels of BCAAs (like leucine, isoleucine, and valine), fasting insulin levels, and HOMA-IR in a model of P. gingivalis-induced periodontitis in HFD-fed mice when compared to uninfected HFD-fed mice (Tian et al., 2020). This supports the idea that high levels of BCAAs in the blood could raise the risk of T2DM (De Bandt et al., 2022) by activating mammalian target of rapamycin (mTOR) and then ribosomal protein S6 kinase1 (S6K1), which phosphorylates insulin receptor substrate 1 (IRS-1) and promotes insulin resistance (Yoon, 2016; White et al., 2021; Figure 2).

In summary, the oral microbiome may enhance HFD-induced insulin resistance by generating specific antibodies against oral pathogens and producing unique amino acids, which provides new research directions for understanding the development of MetS. However, translating conclusions from HFD mouse models to human MetS research faces certain obstacles. This heterogeneity and limitations primarily stem from genetic backgrounds, dietary design, intervention time scales, and microbiome differences, among other factors (Liu et al., 2021). Approaches to bridge this gap include humanization, multi-factor induced mouse models (e.g., high-fat + high-sugar + stress), extending intervention durations, and validation using more complex animal models and human cohorts (Codazzi et al., 2024; Lone et al., 2023).

 2.1.4 Oral microbiome may contribute to insulin resistance through oral-gut axis

Existing research has suggested that the gut microbiome plays a part in the pathophysiology of MetS (Dabke et al., 2019), which has expanded to the oral-gut axis and its involvement in a number of disorders (Kunath et al., 2024). Under normal conditions, the oral-gut axis maintains homeostasis, preventing most oral-derived pathogenic microorganisms from bypassing barriers such as gastric acid, digestive enzymes, and intestinal epithelial tight junctions, thereby avoiding their ectopic colonization in the gut (Kunath et al., 2024). However, in certain scenarios, such as host susceptibility to colitis or pre-existing intestinal inflammation, oral pathogen enrichment and ectopic colonization in the gut may contribute to systemic diseases and insulin resistance (Yamazaki and Kamada, 2024).

Niu et al. (2024) found that the oral-gut translocation of viable P. gingivalis functions as a fuel connecting periodontitis and insulin resistance, involving the reduction of aryl hydrocarbon receptor (AhR) ligands and the inactivation of AhR signaling, in a periodontitis mouse model using oral ligature plus P. gingivalis inoculation. Insulin resistance associated with periodontitis was reduced by supplementing with an AhR agonist—Ficz (6-formylindolo (3,2-b) carbazole), where the restoration of gut barrier function may have been a significant factor. Similar to this, oral infection of HFD-fed mice with the periodontal pathogen A. actinomycetemcomitans resulted in changes in the gut microbiome and mediated insulin resistance, liver steatosis, and glucose intolerance in the setting of maladaptation of the oral–gut–liver axis (Komazaki et al., 2017). These evidences suggest that under adverse conditions such as host susceptibility, oral microbiome may facilitate the ectopic colonization of oral pathogens in the gut by compromising the oral-gut barrier, which may contribute to insulin resistance.

Together, the possible approaches by which insulin resistance can be modulated by the oral microbiome involve its lipid metabolism, production of oral pathogen-specific antibodies, special amino acid synthesis, and ectopic colonization through oral-gut axis (Figure 2). Together, these processes add proof for the link between insulin resistance and dysbiosis of the oral microbiome.

 2.2 Systemic inflammation and oral microbiome dysbiosis correlates

Systemic inflammation is another critical component in the pathogenesis of MetS. Firstly, expanded visceral adipose tissue releases higher levels of pro-inflammatory factors [e.g., tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP)] while reducing anti-inflammatory factors (e.g., adiponectin). These inflammatory cytokines, in turn, promote lipolysis in adipose tissue, generating more FFAs and exacerbating insulin resistance and dyslipidemia. Additionally, inflammatory factors can directly induce insulin resistance through various downstream signaling pathways, which further sustains systemic inflammation (Fahed et al., 2022). These mechanisms collectively form the foundation of MetS pathogenesis.

2.2.1 Oral microbial dysbiosis is usually accompanied by elevated systemic inflammatory markers

The oral microbiome and systemic inflammation have already been linked in a number of observational studies. In contrast to healthy controls, patients with severe periodontitis have been found to have higher blood levels of neutrophils and pro-inflammatory mediators (such as IL-1, IL-6, CRP, and fibrinogen; Schenkein et al., 2020; Teles et al., 2024). Another cross-sectional study involving 70 participants found that serum high-sensitivity CRP and fibrinogen levels were correlated with the severity of periodontitis, with the association strengthening as the severity of periodontal disease increases (Andreu et al., 2021).

Moreover, local periodontal treatment has been found to attenuate systemic inflammatory markers (e.g., CRP, TNF-α) and improve comorbid disease activity (D'Aiuto et al., 2018). In a multicenter longitudinal study involving 153 participants, researchers found that serum IL-6 levels were successfully lowered by periodontal therapy in systemically healthy patients with periodontal disease (Matsuda et al., 2024).

These findings suggest that higher levels of systemic inflammatory markers are associated with a disturbance of oral microbial balance, which may contribute to systemic inflammation.

 2.2.2 Oral microbiome dysbiosis can mediate systemic inflammation through various mechanisms

A wide range of studies hold the opinion that there exists a bidirectional relationship between systemic inflammation and oral microbial dysbiosis. Some indicated that systemic inflammation promote the growth of certain inflammophilic bacteria by providing tissue degradation products (Hajishengallis and Chavakis, 2021), thereby modulating oral microbial dysbiosis. However, their reciprocal correlation requires further elucidation. Here, we focus on the oral microbiome and examine evidence indicating that oral dysbiosis may induce inflammatory responses. This perspective may help establish connections between the oral microbiome and MetS from a pathogenic standpoint.

It is commonly known that oral dysbiosis and local inflammation are related. Payne et al. discovered that the dysbiotic oral microbiome caused by P. gingivalis could be vertically transmitted from parents to their children and stably transferred to germ-free mice. This resulted in the development of periodontitis, indicating that the dysbiotic microbiome may be a direct source of local inflammatory pathology (Payne et al., 2019; Li et al., 2023b).

Oral pathogens may enter the bloodstream through tissue damage caused by local inflammation, leading to transient but frequent bacteremia. This bacteremia allows oral pathogens to disseminate systemically, either directly colonizing distant sites to mediate disease or inducing endotoxemia, which stimulates the immune system to produce inflammatory cytokines and unique immune system alterations, subsequently affecting systemic inflammation (Hajishengallis and Chavakis, 2021; Plachokova et al., 2021; Figure 3).

Impact of oral microbiome on systemic inflammation and adipokines secretion. The oral microbiome modulates the “central inflammation” hub function of bone marrow by altering its immune status, and influences systemic inflammation through cellular signaling pathways. Concurrently, inflammation itself regulates adipose tissue and affects adipokines secretion. Visual guide: Black arrows: Represent progressive relationships; Blue arrows: Show pathway modifications; Blue background: Highlights MetS mechanisms and phenotypes discussed in this study; Yellow background: Marks relevant signaling pathways; Green background: Shows adipokines secretion. Abbreviations: STAT3, signal transducers and activators of transcription 3; NF-κB nuclear factor-κB; VCAM 1, vascular cell adhesion molecule 1; CAP 1, caspase recruitment domain-containing protein 1; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; CRP, C-reactive protein. Created in BioRender. Yue, Z. (2025) https://BioRender.com/3m6ifm3.

Illustration showing the process of systemic inflammation initiated by the oral microbiome. This involves trained myelopoiesis in bone marrow and the STAT3 pathway, leading to hyperactive myeloid cells with NF-kB p65 nuclear translocation elevated, VCAM1 expression increased, and more adhesion to endothelial cells. At the same time, CAP+ monocytes extravasation increased. Inflammatory cytokines such as IL-6, TNF-?, and CRP are released, affecting adipose tissue. Adipokines secretion is altered, increasing leptin and resistin and decreasing adiponectin. 2.2.2.1 Oral microbiome may promote systemic inflammation by activating immune cells and altering signaling pathways

Hu et al. (2021) found that, in ligature-induced periodontitis (LIP) rats, peripheral blood and the brain showed apparent elevations in inflammatory cytokines (IL-1β, IL-6, IL-8, and IL-21), which are mediated by the signal transducers and activators of transcription 3 (STAT3) signaling pathway. According to a different study by Lorena et al., LIP in rats caused endothelial dysfunction and vascular inflammation, which were accompanied by elevated neutrophil counts and elevated blood levels of IL-6 and CRP (Brito et al., 2013). Periodontitis-induced activation of circulating monocytes, their increased adherence to aortic endothelial cells via nuclear factor-κB (NF-κB) p65, and the elevation of vascular cell adhesion molecule 1 (VCAM1) in the latter are considered to be contributing factors to vascular inflammation (Miyajima et al., 2014; Shen et al., 2023; Kim et al., 2023). According to these investigations on animals, the oral microbiome may encourage systemic inflammation through its specific immune responses and associated downstream signaling pathway alterations (Figure 3).

 2.2.2.2 Oral microbiome modulates systemic inflammation via bone marrow

Periodontal disease appears to shift the bone marrow toward a state of trained myelopoiesis, which refers to elevated levels of hyper-reactive myeloid cells with higher capacity to produce pro-inflammatory cytokines, formimg a mechanistic basis of systematic inflammation (Li et al., 2022). The correlation between periodontal and cardiovascular inflammation may be partially explained by increased hematopoietic activity, as demonstrated by 18F-fluorodeoxyglucose positron emission tomography–computed tomography (18F-FDG-PET-CT) investigations (Ishai et al., 2019; Arefnia et al., 2024), indicating that periodontitis may trigger inflammatory adaptation of HSPCs and trained immunity in the bone marrow. It can be speculated that its ability to influence cardiovascular inflammation may partially explain the phenomenon where oral microbial dysbiosis is associated with an increased risk of CVDs (Tonelli et al., 2023). In accordance with the idea of “central inflammation,” this increased bone marrow inflammatory activity might contribute to the oral microbiome's encouragement of systemic inflammation (Chavakis et al., 2019; Figure 3).

 2.2.2.3 The oral microbiome may affect systemic inflammation through oral-gut axis

Recent studies indicate that the oral-gut axis plays an important role in systemic inflammation associated with oral dysbiosis. Oral bacteria may disseminate via the oropharyngeal or oro-digestive route (Hajishengallis et al., 2023). As previously stated, ectopic colonization of the gut by oral organisms may mediate inflammation, alter the local microbial composition, and impair the function of the gut barrier, all of which may induce endotoxemia and modulate systemic inflammation (Kitamoto et al., 2020b; Yu et al., 2025).

From the perspective of immune cells, periodontitis, and colitis are linked via T cell priming in the periodontal tissue and subsequent T cell trafficking to the gut in mice (Kitamoto et al., 2020b). In particular, oral pathobiont-specific T lymphocytes, which proliferate during LIP, move from the cervical lymph nodes that drain the oral cavity to the gut and express the gut-homing markers α4β7 integrin (the receptor for the gut-specific vascular addressin MAdCAM1) and CC-chemokine receptor 9 (CCR9; Kitamoto et al., 2020b). These oral pathobiont-specific T cells, in which T helper (Th) 17 cells are enriched, multiply, and worsen colitis once they reach the gut (Kitamoto et al., 2020b). This represents the mechanisms linking oral microbiome to IBD. Therefore, it is plausible to believe that the oral-gut axis is essential to the procedure by which the oral microbiome mediates systemic inflammation (Figure 2).

In summary, the oral microbiome can mediate systemic inflammation by modulating signaling pathways, activating immune cells, enhancing bone marrow inflammatory activity, and altering gut microbiome homeostasis. Compared to animal studies with strictly controlled variables, human research involves certain confounding factors that require discussion. Smoking has been demonstrated to elevate systemic inflammation levels (Elisia et al., 2020), and most of the cited human studies controlled for smoking as a covariate. Even after adjusting for smoking, the studies still support that alterations in the oral microbiome itself are associated with systemic inflammation. Unfortunately, existing research has rarely thoroughly investigated baseline conditions such as dietary habits and oral hygiene, which influence the oral microbiome. It is well-established that good oral hygiene practices can reduce the incidence of dental caries and periodontitis (Zini et al., 2021; Mutluay and Mutluay, 2022). Furthermore, Liu et al. (2023) found that poor oral hygiene increases the risk of nasopharyngeal carcinoma by altering the oral microbiome. Given these overlooked potential confounders, it is reasonable to suspect that these unaccounted variables could bias the research outcomes. Future studies should aim to improve in these aspects.

 2.3 The oral microbiome's function in the secretion of adipokines

The adipose tissue is recognized to be not only a thermoregulator and lipid storage facility, but also an endocrine organ which plays a crucial role in the pathogenesis of MetS (Fahed et al., 2022). The former primarily refers to the elevated levels of FFAs released by it, which contributes to pro-atherogenic dyslipidemia during the expansion of visceral adiposity in MetS. The latter involves various adipokines released by adipose tissue including peptides [such as resistin and plasminogen activator inhibitor (PAI)-1], hormones (e.g., leptin and adiponectin), and inflammatory cytokines (including chemerin, TNF-α, IL-6, and visfatin; Taylor, 2021; Kim et al., 2022).

Leptin, a multi-target organ protein hormone secreted by adipose tissue. A pro-inflammatory immune response is known to be facilitated by leptin via various immune cells (Liu and Li, 2025). The idea of “leptin resistance,” in which tissues have reduced sensitivity to leptin, was born out of the inability of high leptin levels to address the metabolic imbalance observed in obesity (Obradovic et al., 2021). Elevated levels of leptin are associated with increased risk of MetS (Pappas-Gogos et al., 2022). In an observational cross-sectional study involving 102 participants, patients with early rheumatoid arthritis exhibited higher serum leptin levels, and the presence of gingivitis or periodontal disease markers was found to influence leptin levels (Rodríguez et al., 2021). Another 6-year follow-up study in youth showed that LPS was associated with higher blood leptin levels (Perng et al., 2022).

Based on the previously discussed impact of the oral microbiome on systemic inflammation, we can speculate that oral microbial dysbiosis may contribute to abnormal leptin levels/leptin resistance through modulating systemic inflammatory factors, endotoxemia, and immune cells.

Resistin, a cytokine released by immune cells and adipocytes, is mostly correlated with metabolic disorders, inflammation, and insulin resistance (Kamil et al., 2023). In a single-cell RNA analysis study involving 27 participants, researchers found that the resistin pathway was intensified in individuals with periodontitis and those with both periodontitis and diabetes. By attaching to receptors and triggering several intracellular signaling pathways [NF-κB, mitogen-activated protein kinase (MAPK), and AMP-activated protein kinase (AMPK)], especially through caspase recruitment domain-containing protein 1 (CAP1)+ classical monocytes, resistin may be responsible for this phenomenon, which makes periodontitis a precursor to T2DM (Lee et al., 2023).

Conversely, the hormone adiponectin, which is released by adipocytes, has anti-inflammatory, insulin-sensitive, and metabolism-regulating qualities (Nesic et al., 2022; Petersen et al., 2024). Tang et al. (2022) hold the opinion that specific single nucleotide polymorphisms in the adiponectin gene may be identified as susceptibility genes for T2DM. In a cohort study involving 71 subjects, Alkan and Guzeldemir-Akcakanat (2021) found that by modifying the amounts of adipokines (IL-1β, TNF-α, leptin, resistin, and adiponectin) in the serum, saliva, and gingival crevicular fluid of obese female patients, different severity of periodontal disease affected general health. Furthermore, in vitro experiments observed that administration of adiponectin receptor agonists reduced the levels of TNF and IL-6 (Shinohara et al., 2022). Thus, oral dysbiosis may reduce the adiponectin levels through systematic inflammation, forming a bidirectional relationship between the latter two.

Additionally, whether periodontal therapy can reverse abnormal adipokine levels in patients with oral dysbiosis requires further validation. Multiple systematic reviews have investigated changes in adipokine and inflammatory marker levels (in serum/gingival crevicular fluid/saliva) of periodontitis patients before and after non-surgical periodontal therapy (NSPT). While some concluded that NSPT could improve dysregulated adipokine profiles (Zhang et al., 2023; Tajik et al., 2025; de Menezes et al., 2024), negative findings have been reported (Zhu et al., 2017). These discrepancies may stem from variations in inclusion/exclusion criteria across different systematic reviews, necessitating further exploration and verification through independent clinical trials.

In conclusion, the oral microbiome may influence adipokine levels through inflammatory responses, signaling pathways and the immune system (Figure 3). The changes in these adipokines seem to be synergistic—pro-inflammatory factors decrease while anti-inflammatory factors increase (as shown in Table 1), which may contribute to the pathogenesis of MetS.

The role and changes of adipokines in normal condition versus oral dysbiosis.

Adipokines Normal condition Oral dysbiosis
Leptin Pro-inflammatory ↑ and/or leptin resistance
Resistin Pro-inflammatory ↑
Adiponectin Anti-inflammatory ↓
Visfatin Pro-inflammatory ↑
Chemerin Pro-inflammatory ↑
Omentin-1 Pro-inflammatory ↑
2.4 Oral microbiome may mediate hypertension through modifying nitric oxide (NO) metabolism

NO is essential to the onset and advancement of hypertension. As a key vasoactive molecule, NO controls endothelial function, inflammatory responses, and vascular tone, all of which affect blood pressure (Bryan, 2022).

According to a study using data from the National Health and Nutrition Examination Survey (NHANES), there may be a connection between the prevalence of hypertension, periodontal disease, and oral health (Li et al., 2023a). In a similar vein, a short-term prospective cohort study of 2,588 Japanese students also discovered a strong correlation between hypertension and periodontal disease (Kawabata et al., 2016).

The positive effects of nitrate-rich meals on blood pressure may be mediated by oral bacteria, especially nitrate-reducing bacteria (e.g., Streptococcus and Veillonella genera). These bacteria play a part in NO production, and their reduction caused by oral dysbiosis may impair NO-mediated vasodilation, which, at least in part, leads to the development of hypertension (Alzahrani et al., 2021; Barbadoro et al., 2021). Meanwhile, dietary nitrate supplementation can alter the oral microbiome in individuals with hypertension, exerting potential prebiotic effects (du Toit et al., 2024). Furthermore, longitudinal studies suggest that excessive oral hygiene practices (such as prolonged use of over-the-counter antimicrobial mouthwashes) may reduce the abundance of oral nitrate-reducing bacteria, leading to decreased NO production and potentially increasing hypertension risk (Joshipura et al., 2020). This finding was confirmed in exploratory experiments, where 7-day use of chlorhexidine mouthwash altered the oral microbiome of healthy subjects, reduced nitrite concentrations in both saliva and plasma, and resulted in elevated systolic blood pressure (Bescos et al., 2020).

Meanwhile, the development of hypertension is also partially influenced by insulin resistance. Studies have shown that during insulin resistance, the vasodilatory effects of insulin are reduced, while its activation of the sympathetic nervous system and renin-induced sodium reabsorption remains intact. These mechanisms collectively contribute to the pathophysiology by which insulin resistance promotes hypertension (Fahed et al., 2022). Therefore, based on the earlier discussion of how the oral microbiome may promote insulin resistance, it is reasonable to suspect that the oral microbiome may indirectly affect the development of hypertension by inducing insulin resistance.

Moreover, the impact of periodontal therapy on blood pressure has been explored in numerous randomized controlled trials (RCTs) and systematic reviews (Zhou et al., 2017; Shang et al., 2025). Evidence of varying quality suggests that periodontal treatment may have short-term beneficial effects on subjects' blood pressure, but there is a lack of long-term evaluation of this intervention (Muñoz Aguilera et al., 2020; Sharma et al., 2021; Meng et al., 2024; Orlandi et al., 2022). The systematic review by Luo et al. (2021) concluded that current evidence is insufficient to demonstrate definitive blood pressure-lowering effects from periodontal therapy, highlighting the need for longer-term, high-quality studies to validate the benefits of oral health improvement on hypertension.

In conclusion, it is reasonable to suspect that the oral microbiome plays a potential role in the development of hypertension by producing NO and indirectly through insulin resistance (Figure 2).

 3 Targeting oral microbiome for the treatment of MetS

Current research on microbiota-based therapies primarily focuses on restoring gut microbiome homeostasis, mainly through the supplementation of probiotics, prebiotics, fecal microbiota transplantation (FMT), and localized microbial modulator. Exploration of oral microbiome-based therapies follows a similar approach, though current research in this area remains limited.

Research has shown that eating habits have a big impact on the oral microbiome's composition (Vach et al., 2022). A high-fiber diet aids in weight loss for obese individuals, improves blood glucose levels and insulin sensitivity in both non-diabetic and diabetic individuals, and reduces blood pressure and serum cholesterol levels, offering benefits for various components of MetS (Anderson et al., 2009; Lepping et al., 2022). By lowering the amount of Alloprevotella in the oral microbiome, Sato et al. (2024) found that a high-fiber diet with a high 12-component modified Japanese diet index (mJDI12) may enhance overall health. However, there are certain challenges in modulating the oral microbiome through dietary interventions. Individual genetic backgrounds, lifestyle variations, and compliance issues may hinder both the adoption of dietary changes and their effectiveness. Moreover, current research lacks long-term dynamic studies on oral microbiome changes following dietary modifications, and the mechanisms by which specific nutrients regulate key microbial species remain unclear (Kerstens et al., 2024). Additionally, the metabolic transformation of dietary components under the influence of the oral microbiome has not been fully elucidated (Bai et al., 2025; Shoer et al., 2023). Future research should focus on elucidating the tripartite interaction mechanisms among the oral microbiome, diet, and host, while developing tailored dietary modification strategies for different eating patterns and investigating their long-term effects.

Strategies using probiotics/prebiotics to restore oral microbiome homeostasis have been explored. Probiotics that produce nisin or nisin itself can change the oral microbiome for the better, reducing periodontal destruction and host immune responses (Gao et al., 2022; Nguyen et al., 2020). By dramatically reducing the messenger RNA (mRNA) expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in the brain that are raised by periodontal infection, these probiotics also help neuroinflammation that resembles Alzheimer's disease and is brought on by periodontal disease (Zhao et al., 2023). According to Rosier et al., people with diabetes and hypertension may benefit from taking supplements containing nitrate/symbiotic combos (nitrate + nitrate-reducing probiotics). However, individual variations in oral microbiome's nitrate-reduction capacity exist. Some people may therefore benefit directly from nitrate as a prebiotic because of their microbiota's innate capacity to significantly reduce nitrate, while others may benefit more from symbiotic pairings (Rosier et al., 2020). Therefore, it is necessary to emphasize personalized microbiome-based therapies in the future. Two double-blind, placebo-controlled trials from the same research team (Yarahmadi et al., 2024; Bazyar et al., 2020) collectively demonstrate that combining symbiotic supplementation with NSPT may help improve inflammation, antioxidant status, periodontal health, glycemic control, and reduce LDL- cholesterol in patients with T2DM and chronic periodontitis. The study by Zorina et al. (2017) likewise revealed similar results. Therefore, from the perspectives of inflammation and microbial metabolism, supplementing with probiotics/prebiotics helps restore oral microbiome homeostasis and promote beneficial metabolic processes, potentially offering benefits for MetS. Nevertheless, potential risks associated with probiotic/prebiotic supplementation warrant careful consideration, including unintended microbiome alterations that may lead to opportunistic infections, or horizontal transfer of antibiotic resistance genes (Ji et al., 2023; Merenstein et al., 2023). Future long-term longitudinal studies are imperative to thoroughly evaluate the safety profile and intervention robustness of this approach (Wieërs et al., 2019).

Corresponding to FMT, the oral microbiota transplantation (OMT) has been introduced (Nascimento, 2017; Beikler et al., 2021). In animal experiments, Xiao et al. (2021) showed that OMT helps mice with head and neck cancer who had radiation-induced oral mucositis. Subsequent clinical research by Goloshchapov et al. (2024) found that maternal saliva transplantation prevented severe chemotherapy-induced oral mucositis, accompanied by changes in the composition of the patient's oral microbiome, with no negative transplant-related events noted. However, the implementation of OMT currently faces significant challenges. Unlike the well-established FMT, there is no internationally recognized standardized protocol for OMT (Bokoliya et al., 2021). Critical gaps exist in multiple aspects, including donor screening, transplantation routes, administration protocols, complication prevention, colonization dynamics of transplanted microbes, and ethical oversight (Allegretti et al., 2020). These obstacles primarily stem from the insufficient depth, breadth, and long-term scope of oral microbiome research itself. It is anticipated that they can be gradually resolved as the field advances in the future. Despite these limitations, OMT remains a promising candidate for future oral microbiome-based interventions.

These encouraging findings suggest that microbiota-based therapies targeting the oral microbiome are a promising and worthwhile direction for exploring MetS treatment. However, further research is needed to refine aspects such as dosage, treatment duration, efficacy, adverse effects, and long-term outcomes.

 4 Discussion

In this review, we have summarized the possible contributions of the oral microbiome to the pathogenesis of MetS and described therapeutic explorations targeting oral homeostasis. Nonetheless, there is no denying that oral microbiome dysbiosis and MetS are correlated in both directions (Dame-Teixeira et al., 2025). Future research should prioritize well-designed RCTs to establish causal relationships between the oral microbiome and incident MetS. Additionally, integrating multi-omics approaches (e.g., metagenomics, metabolomics, and proteomics) could further elucidate the underlying mechanisms and strengthen the evidence for causality.

Longitudinal lifespan studies in both animal models and human populations are required to forecast the long-term consequences of microbiome-based interventions (Hsu et al., 2021; Depommier et al., 2019). Optimization of more comprehensive and robust animal models of MetS are needed as well.

Moreover, the structure of oral microbiome varies across different niches (Baker et al., 2024). Most of the current research on oral microbiome comes from studies on periodontal disease and dental plaque, while other specimens need to include saliva, oral rinses, buccal mucosal, dorsum tongue swabs, and etc. (Jiao et al., 2022; Arweiler et al., 2021; Chan et al., 2021) The link between the oral microbiome at distinct oral niches and different diseases should be one of the main focuses of future research.

To sum up, this study methodically describes how the oral microbiome may contribute to the pathogenesis of MetS, offering important new information for future studies on the connection between systemic disorders and the oral microbiome. Though further research is required to convert these findings into therapeutic applications, studies on the oral microbiome show promise for conquering MetS.

 Author contributions

ZY: Writing – original draft. YF: Methodology, Writing – review & editing, Supervision. GS: Funding acquisition, Writing – review & editing. XC: Supervision, Funding acquisition, Methodology, Conceptualization, Writing – review & editing.

 Funding

The author(s) declare that financial support was received for the research and/or publication of this article. This work was funded by the CAMS Innovation Fund for Medical Sciences (CIFMS) [2021-I2M-1-023, 2023-I2M-C&T-B-005], Science & Technology Fundamental Resources Investigation Program [Grant No. 2022FY100800], National High Level Hospital Clinical Research Funding [2022-PUMCH-B-094].

 Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Generative AI statement

The author(s) declare that no Gen AI was used in the creation of this manuscript.

 Publisher's note

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 References Alkan B. Guzeldemir-Akcakanat E. (2021). Evaluation of adipokine levels in obese women with periodontitis: a Cohort study. Am. J. Dent. 34, 267–272. Allegretti J. R. Kassam Z. Mullish B. H. Chiang A. Carrellas M. Hurtado J. . (2020). Effects of fecal microbiota transplantation with oral capsules in obese patients. Clin. Gastroenterol. Hepatol. 18, 855–863.e2. 10.1016/j.cgh.2019.07.00631301451 Alzahrani H. S. Jackson K. G. Hobbs D. A. Lovegrove J. A. (2021). The role of dietary nitrate and the oral microbiome on blood pressure and vascular tone. Nutr. Res. Rev. 34, 222–239. 10.1017/S095442242000028133280615 Anderson J. W. Baird P. Davis R. H. Ferreri S. Knudtson M. Koraym A. . (2009). Health benefits of dietary fiber. Nutr. Rev. 67, 188–205. 10.1111/j.1753-4887.2009.00189.x19335713 Andreu R. Santos-del-Riego S. Payri F. (2021). Serum inflammatory and prooxidant marker levels in different periodontal disease stages. Healthcare-Basel 9:1070. 10.3390/healthcare908107034442206 Arefnia B. Horina A. Nazerani-Zemann T. Seinost G. Rieder M. Wimmer G. . (2024). Nuclear imaging to visualize periodontal inflammation: findings of a randomized controlled trial. Oral Dis. 30, 4630–4638. 10.1111/odi.1491638438328 Arweiler N. B. Rahmel V. Alhamwe B. A. Alhamdan F. Zemlin M. Boutin S. . (2021). Dental biofilm and saliva microbiome and its interplay with pediatric allergies. Microorganisms 9:1330. 10.3390/microorganisms906133034207425 Bai J. Tian Y. Lu Y. Chen Y. Yu M. Gao X. . (2025). Differential effects of high-fat diet on salivary and gut microbiota. Front. Cell. Infect. Microbiol. 15:1547555. 10.3389/fcimb.2025.154755540066066 Baker J. L. Mark Welch J. L. Kauffman K. M. McLean J. S. He X. (2024). The oral microbiome: diversity, biogeography and human health. Nat. Rev. Microbiol. 22, 89–104. 10.1038/s41579-023-00963-637700024 Barbadoro P. Ponzio E. Coccia E. Prospero E. Santarelli A. Rappelli G. G. L. . (2021). Association between hypertension, oral microbiome and salivary nitric oxide: a case-control study. Nitric Oxide 106, 66–71. 10.1016/j.niox.2020.11.00233186726 Bazyar H. Maghsoumi-Norouzabad L. Yarahmadi M. Gholinezhad H. Moradi L. Salehi P. . (2020). The impacts of synbiotic supplementation on periodontal indices and biomarkers of oxidative stress in type 2 diabetes mellitus patients with chronic periodontitis under non-surgical periodontal therapy. A double-blind, placebo-controlled trial. Diabetes Metab. Syndr. Obes. 13, 19–29. 10.2147/DMSO.S23006032021348 Beikler T. Bunte K. Chan Y. Weiher B. Selbach S. Peters U. . (2021). Oral microbiota transplant in dogs with naturally occurring periodontitis. J. Dent. Res. 100, 764–770. 10.1177/002203452199542333733913 Bescos R. Ashworth A. Cutler C. Brookes Z. L. Belfield L. Rodiles A. . (2020). Effects of chlorhexidine mouthwash on the oral microbiome. Sci. Rep. 10:5254. 10.1038/s41598-020-61912-432210245 Blasco-Baque V. Garidou L. Pomié C. Escoula Q. Loubieres P. Le Gall-David S. . (2017). Periodontitis induced by porphyromonas gingivalis drives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response. Gut 66, 872–885. 10.1136/gutjnl-2015-30989726838600 Bokoliya S. C. Dorsett Y. Panier H. Zhou Y. (2021). Procedures for fecal microbiota transplantation in murine microbiome studies. Front. Cell. Infect. Microbiol. 11:711055. 10.3389/fcimb.2021.71105534621688 Brito L. C. W. DalBó S. Striechen T. M. Farias J. M. Olchanheski L. R. Mendes R. T. . (2013). Experimental periodontitis promotes transient vascular inflammation and endothelial dysfunction. Arch. Oral Biol. 58, 1187–1198. 10.1016/j.archoralbio.2013.03.00923583017 Brookes Z. Teoh L. Cieplik F. Kumar P. (2023). Mouthwash effects on the oral microbiome: are they good, bad, or balanced? Int. Dent. J. 73, S74–81. 10.1016/j.identj.2023.08.01037867065 Bryan N. S. (2022). Nitric oxide deficiency is a primary driver of hypertension. Biochem. Pharmacol. 206:115325. 10.1016/j.bcp.2022.11532536349641 Chan J. Y. K. Ng C. W. K. Lan L. Fung S. Li J.-W. Cai L. . (2021). Restoration of the oral microbiota after surgery for head and neck squamous cell carcinoma is associated with patient outcomes. Front. Oncol. 11:737843. 10.3389/fonc.2021.73784334692514 Chavakis T. Mitroulis I. Hajishengallis G. (2019). Hematopoietic progenitor cells as integrative hubs for adaptation to and fine-tuning of inflammation. Nat. Immunol. 20, 802–811. 10.1038/s41590-019-0402-531213716 Chen B.-Y. Lin W.-Z. Li Y.-L. Bi C. Du L.-J. Liu Y. . (2023). Roles of oral, microbiota and oral-gut microbial transmission in hypertension. J. Adv. Res. 43, 147–161. 10.1016/j.jare.2022.03.00736585105 Codazzi V. Frontino G. Galimberti L. Giustina A. Petrelli A. (2024). Mechanisms and risk factors of metabolic syndrome in children and adolescents. Endocrine 84, 16–28. 10.1007/s12020-023-03642-x38133765 Costa C. F. F. A. Correia-de-Sá T. Araujo R. Barbosa F. Burnet P. W. J. Ferreira-Gomes J. . (2024). The oral-gut microbiota relationship in healthy humans: identifying shared bacteria between environments and age groups. Front. Microbiol. 15:1475159. 10.3389/fmicb.2024.147515939512939 Dabke K. Hendrick G. Devkota S. (2019). The gut microbiome and metabolic syndrome. J. Clin. Invest. 129, 4050–4057. 10.1172/JCI12919431573550 D'Aiuto F. Gkranias N. Bhowruth D. Khan T. Orlandi M. Suvan J. . (2018). Systemic effects of periodontitis treatment in patients with type 2 diabetes: a 12 month, single-centre, investigator-masked, randomised trial. Lancet Diabetes Endocrinol. 6, 954–965. 10.1016/S2213-8587(18)30038-X30472992 Dame-Teixeira N. Do T. Deng D. (2025). The oral microbiome and us. Adv. Exp. Med. Biol. 1472, 3–9. 10.1007/978-3-031-79146-8_140111682 De Bandt J.-P. Coumoul X. Barouki R. (2022). Branched-chain amino acids and insulin resistance, from protein supply to diet-induced obesity. Nutrients 15:68. 10.3390/nu1501006836615726 de Menezes C. C. da Silva Barbirato D. Fogacci M. F. Marañón-Vásquez G. A. Carneiro J. R. I. Maia L. C. . (2024). Systemic benefits of periodontal therapy in patients with obesity and periodontitis: a systematic review. Braz. Oral Res. 38:e031. 10.1590/1807-3107bor-2024.vol38.003138597549 Depommier C. Everard A. Druart C. Plovier H. Van Hul M. Vieira-Silva S. . (2019). Supplementation with akkermansia muciniphila in overweight and obese human volunteers: a proof-of-concept exploratory study. Nat. Med. 25, 1096–1103. 10.1038/s41591-019-0495-231263284 du Toit L. Sundqvist M. L. Redondo-Rio A. Brookes Z. Casas-Agustench P. Hickson M. . (2024). The effect of dietary nitrate on the oral microbiome and salivary biomarkers in individuals with high blood pressure. J. Nutr. 154, 2696–2706. 10.1016/j.tjnut.2024.07.00239019159 Elisia I. Lam V. Cho B. Hay M. Li M. Y. Yeung M. . (2020). The effect of smoking on chronic inflammation, immune function and blood cell composition. Sci. Rep. 10:19480. 10.1038/s41598-020-76556-733173057 Fahed G. Aoun L. Bou Zerdan M. Allam S. Bou Zerdan M. Bouferraa Y. . (2022). Metabolic syndrome: updates on pathophysiology and management in 2021. Int. J. Mol. Sci. 23:786. 10.3390/ijms2302078635054972 Gao L. Kuraji R. Zhang M. J. Martinez A. Radaic A. Kamarajan P. . (2022). Nisin probiotic prevents inflammatory bone loss while promoting reparative proliferation and a healthy microbiome. NPJ Biofilms Microbiomes 8:45. 10.1038/s41522-022-00307-x35672331 George A. K. Narayan V. Kurian N. Joseph A. E. Anil S. A. (2021). Pilot study on glycemia and insulin resistance in patients with severe periodontitis. J. Indian Soc. Periodontol. 25, 393–398. 10.4103/jisp.jisp_419_2034667381 Goloshchapov O. V. Chukhlovin A. B. Bug D. S. Polev D. E. Kosarev O. V. Klementeva R. V. . (2024). Safety, feasibility, and advantages of oral microbiota transplantation: the first clinical case. J. Pediatr. Hematol. Oncol. 46:287. 10.1097/MPH.000000000000289638875447 Greggianin B. F. Marques A. E. M. Amato A. A. de Lima C. L. (2023). Effect of periodontal therapy on insulin resistance in adults with dysglycemia and periodontitis: a systematic review and meta-analysis. Clin. Oral Investig. 27, 1329–1342. 10.1007/s00784-023-04879-636790626 Hajishengallis G. Chavakis T. (2021). Local and systemic mechanisms linking periodontal disease and inflammatory comorbidities. Nat. Rev. Immunol. 21, 426–440. 10.1038/s41577-020-00488-633510490 Hajishengallis G. Lamont R. J. Koo H. (2023). Oral polymicrobial communities: assembly, function, and impact on diseases. Cell Host Microbe 31, 528–538. 10.1016/j.chom.2023.02.00936933557 Hsu C.-N. Hou C.-Y. Hsu W.-H. Tain Y.-. L. (2021). Early-life origins of metabolic syndrome: mechanisms and preventive aspects. Int. J. Mol. Sci. 22:11872. 10.3390/ijms22211187234769303 Hu Y. Zhang X. Zhang J. Xia X. Li H. Qiu C. . (2021). Activated STAT3 signaling pathway by ligature-induced periodontitis could contribute to neuroinflammation and cognitive impairment in rats. J. Neuroinflamm. 18:80. 10.1186/s12974-021-02071-933757547 Huang X. Huang X. Huang Y. Zheng J. Lu Y. Mai Z. . (2023). The oral microbiome in autoimmune diseases: friend or foe? J. Transl. Med. 21:211. 10.1186/s12967-023-03995-x36949458 Imai J. Kitamoto S. Kamada N. (2021). The pathogenic oral–gut–liver axis: new understandings and clinical implications. Expert Rev. Clin. Immunol. 17, 727–736. 10.1080/1744666X.2021.193587734057877 Ishai A. Osborne M. T. El Kholy K. Takx R. A. P. Ali A. Yuan N. . (2019). Periodontal disease associates with arterial inflammation via potentiation of a hematopoietic-arterial axis. JACC Cardiovasc. Imaging 12, 2271–2273. 10.1016/j.jcmg.2019.05.01531326471 Ji J. Jin W. Liu S.-J. Jiao Z. Probiotics L. i. X. (2023). Prebiotics, and postbiotics in health and disease. MedComm 4:e420. 10.1002/mco2.42037929014 Jiao J. Zheng Y. Zhang Q. Xia D. Zhang L. Ma N. . (2022). Saliva microbiome changes in thyroid cancer and thyroid nodules patients. Front. Cell. Infect. Microbiol. 12:989188. 10.3389/fcimb.2022.98918836034695 Joshipura K. Muñoz-Torres F. Fernández-Santiago J. Patel R. P. Lopez-Candales A. (2020). Over-the-counter mouthwash use, nitric oxide and hypertension risk. Blood Press. 29, 103–112. 10.1080/08037051.2019.168027031709856 Jungbauer G. Stähli A. Zhu X. Auber Alberi L. Sculean A. Eick S. . (2022). periodontal microorganisms and Alzheimer disease - a causative relationship? Periodontol 2000 89, 59–82. 10.1111/prd.1242935244967 Kalhan A. C. Kalhan T. A. Romandini M. Bitencourt F. V. Cooray U. M. P. Leite F. R. M. . (2025). Insulin resistance and periodontitis: mediation by blood pressure. J. Periodont. Res. 60, 226–235. 10.1111/jre.1333339123295 Kamil M. A. Peeran S. W. Basheer S. N. Elhassan A. Alam M. N. Thiruneervannan M. . (2023). Role of resistin in various diseases with special emphasis on periodontal and periapical inflammation – a review. J. Pharm. Bioallied Sci. 15:S31. 10.4103/jpbs.jpbs_655_2237654317 Kapila Y. L. (2021). Oral health's inextricable connection to systemic health: special populations bring to bear multimodal relationships and factors connecting periodontal disease to systemic diseases and conditions. Periodontol 2000 87, 11–16. 10.1111/prd.1239834463994 Kawabata Y. Ekuni D. Miyai H. Kataoka K. Yamane M. Mizutani S. . (2016). Relationship between prehypertension/hypertension and periodontal disease: a prospective Cohort study. Am. J. Hypertens. 29, 388–396. 10.1093/ajh/hpv11726208668 Kerstens R. Ng Y. Z. Pettersson S. Jayaraman A. (2024). Balancing the oral–gut–brain axis with diet. Nutrients 16:3206. 10.3390/nu1618320639339804 Kim J.-E. Kim J.-S. Jo M.-J. Cho E. Ahn S.-Y. Kwon Y.-J. . (2022). The roles and associated mechanisms of adipokines in development of metabolic syndrome. Molecules 27:334. 10.3390/molecules2702033435056647 Kim J.-I. Choi C.-H. Chung K.-H. (2021). No association between metabolic syndrome and periodontitis in korean postmenopausal women. Int. J. Environ. Res. Public Health 18:11110. 10.3390/ijerph18211111034769630 Kim S. Y. Kim Y.-J. Kim S. Momeni M. Lee A. Treanor A. . (2023). GV1001 inhibits the severity of the ligature-induced periodontitis and the vascular lipid deposition associated with the periodontitis in mice. Int. J. Mol. Sci. 24:12566. 10.3390/ijms24161256637628753 Kitamoto S. Nagao-Kitamoto H. Hein R. Schmidt T. M. Kamada N. (2020a). The bacterial connection between the oral cavity and the gut diseases. J. Dent. Res. 99, 1021–1029. 10.1177/002203452092463332464078 Kitamoto S. Nagao-Kitamoto H. Jiao Y. Gillilland M. G. Hayashi A. Imai J. . (2020b). The intermucosal connection between the mouth and gut in commensal pathobiont-driven colitis. Cell 182, 447–462.e14. 10.1016/j.cell.2020.05.04832758418 Komazaki R. Katagiri S. Takahashi H. Maekawa S. Shiba T. Takeuchi Y. . (2017). Periodontal pathogenic bacteria, aggregatibacter actinomycetemcomitans affect non-alcoholic fatty liver disease by altering gut microbiota and glucose metabolism. Sci. Rep. 7:13950. 10.1038/s41598-017-14260-929066788 Kunath B. J. De Rudder C. Laczny C. C. Letellier E. Wilmes P. (2024). The oral-gut microbiome axis in health and disease. Nat. Rev. Microbiol. 22, 791–805. 10.1038/s41579-024-01075-539039286 Lee H. Joo J.-Y. Song J.-M. Kim H.-J. Kim Y. H. Park H. R. . (2023). Immunological link between periodontitis and type 2 diabetes deciphered by single-cell RNA analysis. Clin. Transl. Med. 13:e1503. 10.1002/ctm2.150338082425 Lemieux I. Després J.-P. (2020). Metabolic syndrome: past, present and future. Nutrients 12:3501. 10.3390/nu1211350133202550 Lepping K. Adams-Campbell L. L. Hicks J. Mills M. Dash C. (2022). Dietary fiber intake and metabolic syndrome in postmenopausal African American women with obesity. PLoS ONE 17:e0273911. 10.1371/journal.pone.027391136054124 Li X. Wang H. Yu X. Saha G. Kalafati L. Ioannidis C. . (2022). Maladaptive innate immune training of myelopoiesis links inflammatory comorbidities. Cell 185, 1709–1727.e18. 10.1016/j.cell.2022.03.04335483374 Li X. Zhou R. Han Y. Zeng J. Shi L. Mao Y. . (2023b). Silibinin attenuates experimental periodontitis by downregulation of inflammation and oxidative stress. Oxid. Med. Cell. Longev. 2023:5617800. 10.1155/2023/561780036846719 Li Y. Yuan X. Zheng Q. Mo F. Zhu S. Shen T. . (2023a). The association of periodontal disease and oral health with hypertension, NHANES 2009–2018. BMC Public Health 23:1122. 10.1186/s12889-023-16012-z37308938 Liu C. Li X. (2025). Role of leptin and adiponectin in immune response and inflammation. Int. Immunopharmacol. 161:115082. 10.1016/j.intimp.2025.11508240516255 Liu Q.-Y. Liao Y. Wu Y.-X. Diao H. Du Y. Chen Y.-W. . (2023). The oral microbiome as mediator between oral hygiene and its impact on nasopharyngeal carcinoma. Microorganisms 11:719. 10.3390/microorganisms1103071936985292 Liu S. Ma L. Ren X. Zhang W. Shi D. Huo Y. . (2021). A new mouse model of type 2 diabetes mellitus established through combination of high-fat diet, streptozotocin and glucocorticoid. Life Sci. 286:120062. 10.1016/j.lfs.2021.12006234673117 Lone I. M. Nun N. B. Ghnaim A. Schaefer A. S. Houri-Haddad Y. Iraqi F. A. (2023). High-fat diet and oral infection induced type 2 diabetes and obesity development under different genetic backgrounds. Animal Model Exp. Med. 6, 131–145. 10.1002/ame2.1231137026700 Lu H. Zou P. Zhang Y. Zhang Q. Chen Z. Chen F. . (2022). The sampling strategy of oral microbiome. Imeta 1:e23. 10.1002/imt2.2338868567 Lu Z. Li Y. Chowdhury N. Yu H. Syn W.-K. Lopes-Virella M. . (2023). The presence of periodontitis exacerbates non-alcoholic fatty liver disease via sphingolipid metabolism-associated insulin resistance and hepatic inflammation in mice with metabolic syndrome. Int. J. Mol. Sci. 24:8322. 10.3390/ijms2409832237176029 Luo Y. Ye H. Liu W. Lv Z. Jia Y. Li C. . (2021). Effect of periodontal treatments on blood pressure. Cochrane Db Syst Rev. 2021:CD009409. 10.1002/14651858.CD009409.pub234897644 Magrin G. L. Strauss F. J. Benfatti C. A. M. Maia L. C. Gruber R. (2020). Effects of short-chain fatty acids on human oral epithelial cells and the potential impact on periodontal disease: a systematic review of in vitro studies. Int. J. Mol. Sci. 21:4895. 10.3390/ijms2114489532664466 Matsuda S. Shintani T. Miyagawa T. Yumoto H. Komatsu Y. Dewake N. . (2024). Effect of periodontal treatment on reducing chronic inflammation in systemically healthy patients with periodontal disease. Am. J. Med. 137, 273–279.e2. 10.1016/j.amjmed.2023.11.00137984772 Meng R. Xu J. Fan C. Liao H. Wu Z. Zeng Q. . (2024). Effect of non-surgical periodontal therapy on risk markers of cardiovascular disease: a systematic review and meta-analysis. BMC Oral Health 24:692. 10.1186/s12903-024-04433-038877442 Merenstein D. Pot B. Leyer G. Ouwehand A. C. Preidis G. A. Elkins C. A. . (2023). Emerging issues in probiotic safety: 2023 perspectives. Gut Microbes 15:2185034. 10.1080/19490976.2023.218503436919522 Mikami R. Mizutani K. Matsuyama Y. Matsuura T. Kido D. Takeda K. . (2021). Association between periodontal inflammation and serum lipid profile in a healthy population: a cross-sectional study. J. Periodont. Res. 56, 1037–1045. 10.1111/jre.1291734273107 Miyajima S. Naruse K. Kobayashi Y. Nakamura N. Nishikawa T. Adachi K. . (2014). Periodontitis-activated monocytes/macrophages cause aortic inflammation. Sci. Rep. 4:5171. 10.1038/srep0517124893991 Muniyappa R. (2024). Vascular insulin resistance and free fatty acids: the micro-macro circulation Nexus. J. Clin. Endocrinol. Metab. 109, e1671–e1672. 10.1210/clinem/dgae01338181432 Muñoz Aguilera E. Suvan J. Buti J. Czesnikiewicz-Guzik M. Barbosa Ribeiro A. Orlandi M. . (2020). Periodontitis is associated with hypertension: a systematic review and meta-analysis. Cardiovasc. Res. 116, 28–39. 10.1093/cvr/cvz20131549149 Mutluay M. Mutluay A. T. (2022). Caries prevalence, oral health practices/behaviours and dental anxiety levels amongst dental hygiene students: a cross-sectional study. Int. J. Dent. Hyg. 20, 262–272. 10.1111/idh.1257635007395 Nascimento M. M. (2017). Oral microbiota transplant: a potential new therapy for oral diseases. J. Calif. Dent. Assoc. 45, 565–568. 10.1080/19424396.2017.12222506 Nesic J. Ljujic B. Rosic V. Djukic A. Rosic M. Petrovic I. . (2022). Adiponectin and Interleukin-33: possible early markers of metabolic syndrome. J. Clin. Med. 12:132. 10.3390/jcm1201013236614933 Nguyen T. Brody H. Lin G.-H. Rangé H. Kuraji R. Ye C. . (2020). Probiotics, including nisin-based probiotics, improve clinical and microbial outcomes relevant to oral and systemic diseases. Periodontol 2000 82, 173–185. 10.1111/prd.1232431850634 Niu C. Lv W. Zhu X. Dong Z. Yuan K. Jin Q. . (2024). Intestinal translocation of live porphyromonas gingivalis drives insulin resistance. J. Dent. Res. 103, 197–207. 10.1177/0022034523121419538185909 Obradovic M. Sudar-Milovanovic E. Soskic S. Essack M. Arya S. Stewart A. J. . (2021). Leptin and obesity: role and clinical implication. Front. Endocrinol. 12:585887. 10.3389/fendo.2021.58588734084149 Orlandi M. Muñoz Aguilera E. Marletta D. Petrie A. Suvan J. D'Aiuto F. . (2022). Impact of the treatment of periodontitis on systemic health and quality of life: a systematic review. J. Clin. Periodontol. 49(Suppl. 24), 314–327. 10.1111/jcpe.1355434791686 Pappas-Gogos G. Tepelenis K. Goussia A. Tellis C. Fousekis F. Glantzounis G. K. . (2022). Plasma VEGF and leptin values in patients with gastric intestinal metaplasia and metabolic syndrome. Front. Oncol. 12:905168. 10.3389/fonc.2022.90516835712481 Payne M. A. Hashim A. Alsam A. Joseph S. Aduse-Opoku J. Wade W. G. . (2019). Horizontal and vertical transfer of oral microbial dysbiosis and periodontal disease. J. Dent. Res. 98, 1503–1510. 10.1177/002203451987715031560607 Perng W. Friedman J. E. Janssen R. C. Glueck D. H. Dabelea D. (2022). Endotoxin biomarkers are associated with adiposity and cardiometabolic risk across 6 years of follow-up in youth. J. Clin. Endocrinol. Metab. 107, e3018–e3028. 10.1210/clinem/dgac14935276001 Petersen M. C. Smith G. I. Palacios H. H. Farabi S. S. Yoshino M. Yoshino J. . (2024). Cardiometabolic characteristics of people with metabolically healthy and unhealthy obesity. Cell Metab. 36, 745. 10.1016/j.cmet.2024.03.00238569471 Pirih F. Q. Monajemzadeh S. Singh N. Sinacola R. S. Shin J. M. Chen T. . (2021). Association between metabolic syndrome and periodontitis: the role of lipids, inflammatory cytokines, altered host response, and the microbiome. Periodontol 2000 87, 50–75. 10.1111/prd.1237934463996 Plachokova A. S. Andreu-Sánchez S. Noz M. P. Fu J. Riksen N. P. (2021). Oral microbiome in relation to periodontitis severity and systemic inflammation. Int. J. Mol. Sci. 22:5876. 10.3390/ijms2211587634070915 Pulido-Moran M. Bullon P. Morillo J. M. Battino M. Quiles J. L. Ramirez-Tortosa M. (2017). The relationship between insulin resistance and periodontitis is not affected by mediterranean diet in a Spanish population. Arch. Oral Biol. 77, 62–67. 10.1016/j.archoralbio.2017.01.02328178586 Rodríguez J. Lafaurie G. I. Bautista-Molano W. Chila-Moreno L. Bello-Gualtero J. M. Romero-Sánchez C. . (2021). Adipokines and periodontal markers as risk indicators of early rheumatoid arthritis: a cross-sectional study. Clin. Oral Investig. 25, 1685–1695. 10.1007/s00784-020-03469-032740810 Rosier B. T. Moya-Gonzalvez E. M. Corell-Escuin P. Mira A. (2020). Isolation and characterization of nitrate-reducing bacteria as potential probiotics for oral and systemic health. Front. Microbiol. 11:555465. 10.3389/fmicb.2020.55546533042063 Saklayen M. G. (2018). The global epidemic of the metabolic syndrome. Curr. Hypertens. Rep. 20:12. 10.1007/s11906-018-0812-z29480368 Sanz M. Marco Del Castillo A. Jepsen S. Gonzalez-Juanatey J. R. D'Aiuto F. Bouchard P. . (2020). Periodontitis and cardiovascular diseases: consensus report. J. Clin. Periodontol. 47, 268–288. 10.1111/jcpe.1318932011025 Sato S. Chinda D. Iino C. Sawada K. Mikami T. Nakaji S. . (2024). A Cohort study of the influence of the 12-component modified japanese diet index on oral and gut microbiota in the Japanese general population. Nutrients 16:524. 10.3390/nu1604052438398848 Schamarek I. Anders L. Chakaroun R. M. Kovacs P. Rohde-Zimmermann K. (2023). The role of the oral microbiome in obesity and metabolic disease: potential systemic implications and effects on taste perception. Nutr. J. 22:28. 10.1186/s12937-023-00856-737237407 Schenkein H. A. Papapanou P. N. Genco R. Sanz M. (2020). Mechanisms underlying the association between periodontitis and atherosclerotic disease. Periodontol 2000 83, 90–106. 10.1111/prd.1230432385879 Shaalan A. Lee S. Feart C. Garcia-Esquinas E. Gomez-Cabrero D. Lopez-Garcia E. . (2022). Alterations in the oral microbiome associated with diabetes, overweight, and dietary components. Front. Nutr. 9:914715. 10.3389/fnut.2022.91471535873415 Shang Y. Jiang Y. Chen Y. Fu H. Li F. (2025). Effect of nonsurgical periodontal therapy on glycemic control in type 2 diabetes patients with chronic periodontitis. Shanghai Kou Qiang Yi Xue 34, 202–207.40550775 Sharma S. Sridhar S. McIntosh A. Messow C.-M. Aguilera E. M. Del Pinto R. . (2021). Periodontal therapy and treatment of hypertension-alternative to the pharmacological approach. A systematic review and meta-analysis. Pharmacol Res. 166:105511. 10.1016/j.phrs.2021.10551133617973 Shen S. Sun T. Ding X. Gu X. Wang Y. Ma X. . (2023). The exoprotein Gbp of fusobacterium nucleatum promotes THP-1 cell lipid deposition by binding to CypA and activating PI3K-AKT/MAPK/NF-κB pathways. J. Adv. Res. 57, 93–105. 10.1016/j.jare.2023.04.00737100345 Shinohara I. Kataoka T. Mifune Y. Inui A. Sakata R. Nishimoto H. . (2022). Influence of adiponectin and inflammatory cytokines in fatty degenerative atrophic muscle. Sci. Rep. 12:1557. 10.1038/s41598-022-05608-x35091650 Shoer S. Shilo S. Godneva A. Ben-Yacov O. Rein M. Wolf B. C. . (2023). Impact of dietary interventions on pre-diabetic oral and gut microbiome, metabolites and cytokines. Nat. Commun. 14:5384. 10.1038/s41467-023-41042-x37666816 Sun Y. Wang J. Guo X. Zhu N. Niu L. Ding X. . (2021). Oleic acid and eicosapentaenoic acid reverse palmitic acid-induced insulin resistance in human HepG2 cells via the reactive oxygen species/JUN pathway. Genom. Proteomics Bioinf. 19, 754–771. 10.1016/j.gpb.2019.06.00533631425 Tajik Z. Mehrafarid H. Bayani M. Almasi-Hashiani A. (2025). Comparison of visfatin levels before and after non-surgical periodontal therapy: a systematic review and meta-analysis. PLoS ONE 20:e0315035. 10.1371/journal.pone.031503539970162 Tang Y.-H. Wang Y.-H. Chen C.-C. Chan C.-J. Tsai F.-J. Chen S.-Y. . (2022). Genetic and functional effects of adiponectin in type 2 diabetes mellitus development. Int. J. Mol. Sci. 23:13544. 10.3390/ijms23211354436362336 Taylor E. B. (2021). The complex role of adipokines in obesity, inflammation, and autoimmunity. Clin. Sci. 135, 731–752. 10.1042/CS2020089533729498 Teles F. R. F. Chandrasekaran G. Martin L. Patel M. Kallan M. J. Furquim C. . (2024). Salivary and serum inflammatory biomarkers during periodontitis progression and after treatment. J. Clin. Periodontol. 51, 1619–1631. 10.1111/jcpe.1404839104016 Tian J. Liu C. Zheng X. Jia X. Peng X. Yang R. . (2020). Porphyromonas gingivalis induces insulin resistance by increasing BCAA levels in mice. J. Dent. Res. 99, 839–846. 10.1177/002203452091103732176550 Tonelli A. Lumngwena E. N. Ntusi N. A. B. (2023). The oral microbiome in the pathophysiology of cardiovascular disease. Nat. Rev. Cardiol. 20, 386–403. 10.1038/s41569-022-00825-336624275 Vach K. Al-Ahmad A. Anderson A. Woelber J. P. Karygianni L. Wittmer A. . (2022). Examining the composition of the oral microbiota as a tool to identify responders to dietary changes. Nutrients 14:5389. 10.3390/nu1424538936558547 Wang W. Yan Y. Yu F. Zhang W. Su S. (2023). Role of oral and gut microbiota in childhood obesity. Folia Microbiol. 68, 197–206. 10.1007/s12223-023-01033-336626083 Weijers R. N. (2022). Free fatty acids, glucose, and insulin in type 2 diabetes mellitus. World J. Diabetes 13, 275–277. 10.4239/wjd.v13.i3.27535432753 White P. J. McGarrah R. W. Herman M. A. Bain J. R. Shah S. H. Newgard C. B. . (2021). Insulin action, type 2 diabetes, and branched-chain amino acids: a two-way street. Mol. Metab. 52:101261. 10.1016/j.molmet.2021.10126134044180 Wieërs G. Belkhir L. Enaud R. Leclercq S. Philippart de Foy J.-M. Dequenne I. . (2019). How probiotics affect the microbiota. Front. Cell. Infect. Microbiol. 9:454. 10.3389/fcimb.2019.0045432010640 Wu J. Zheng M. Zhang M. Pang X. Li L. Wang S. . (2018). Porphyromonas gingivalis promotes 4-nitroquinoline-1-oxide-induced oral carcinogenesis with an alteration of fatty acid metabolism. Front. Microbiol. 9:2081. 10.3389/fmicb.2018.0208130233549 Xiao H. Fan Y. Li Y. Dong J. Zhang S. Wang B. . (2021). Oral microbiota transplantation fights against head and neck radiotherapy-induced oral mucositis in mice. Comput. Struct. Biotechnol. J. 19, 5898–5910. 10.1016/j.csbj.2021.10.02834815834 Yamazaki K. Kamada N. (2024). Exploring the oral-gut linkage: interrelationship between oral and systemic diseases. Mucosal Immunol. 17, 147–153. 10.1016/j.mucimm.2023.11.00638007003 Yan J. Zheng W. Xie S. Yun X. Wang Z. Zhou H. . (2024). Testing the causal association between metabolic syndrome and periodontitis: a two-sample mendelian randomisation study. Int. Dent. J. 75, 707–715. 10.1016/j.identj.2024.10.01839665953 Yao H. Wang Y. Zhang X. Li P. Shang L. Chen X. . (2022). Targeting peroxisomal fatty acid oxidation improves hepatic steatosis and insulin resistance in obese mice. J. Biol. Chem. 299:102845. 10.1016/j.jbc.2022.10284536586435 Yarahmadi M. Javid A. Z. Bazyar H. Yousefimanesh H. A. Nejatian T. Gravand E. . (2024). The effects of synbiotic supplementation along with non-surgical periodontal therapy in improving the metabolic status and inflammatory markers in type 2 diabetes mellitus patients with periodontal disease: a double-blind randomized clinical trial. J. Educ. Health Promot. 13:430. 10.4103/jehp.jehp_1382_2339811858 Yoon M.-S. (2016). The emerging role of branched-chain amino acids in insulin resistance and metabolism. Nutrients 8:405. 10.3390/nu807040527376324 Yu J. Lyu J. Zhu T. Li Y. Xia H. Liu Q. . (2025). Oral-gut axis in inflammation: periodontitis exacerbates ulcerative colitis via microbial dysbiosis and barrier disruption. BMC Oral Health 25:894. 10.1186/s12903-025-06269-840462053 Zhang Y. Jia R. Zhang Y. Sun X. Mei Y. Zou R. . (2023). Effect of non-surgical periodontal treatment on cytokines/adipocytokines levels among periodontitis patients with or without obesity: a systematic review and meta-analysis. BMC Oral Health 23:717. 10.1186/s12903-023-03383-337798684 Zhao C. Kuraji R. Ye C. Gao L. Radaic A. Kamarajan P. . (2023). Nisin a probiotic bacteriocin mitigates brain microbiome dysbiosis and Alzheimer's disease-like neuroinflammation triggered by periodontal disease. J. Neuroinflamm. 20:228. 10.1186/s12974-023-02915-637803465 Zhou Q.-B. Xia W.-H. Ren J. Yu B.-B. Tong X.-Z. Chen Y.-B. . (2017). Effect of intensive periodontal therapy on blood pressure and endothelial microparticles in patients with prehypertension and periodontitis: a randomized controlled trial. J. Periodontol. 88, 711–722. 10.1902/jop.2017.16044728452620 Zhu J. Guo B. Gan X. Zhang L. He Y. Liu B. . (2017). Association of circulating leptin and adiponectin with periodontitis: a systematic review and meta-analysis. BMC Oral Health 17:104. 10.1186/s12903-017-0395-028662701 Zini A. Mazor S. Timm H. Barker M. L. Grender J. M. Gerlach R. W. . (2021). Effects of an oral hygiene regimen on progression of gingivitis/early periodontitis: a randomized controlled trial. Can. J. Dent. Hyg. 55:85.34221032 Zorina O. A. Petrukhina N. B. Kartysheva E. V. Saltovets M. V. (2017). The effectiveness of combined treatment with simbiotiks and antioxidants in patients with periodontal disease and metabolic syndrome. Vopr. Pitan. 86, 87–92. 10.24411/0042-8833-2017-0002530645895