Remarkably, 100 healthy 1-day-old Ross 308 broiler chickens were randomly divided into two treatment groups: a control group and an LGG group, with 5 replicates in each group and 10 chickens in each replicate. The control group of broilers was fed with basic feed, while the LGG group was supplemented with LGG at a dose of 1,000 mg/kg in the basic feed. The experiment lasted for 29 days and was divided into two stages. Experiment 1: The first stage of the experiment lasted for 27 days and was weighed on the 14th and 27th days, respectively, to record the daily feed intake.

Experiment 2: the second stage lasted for 2 days, from day 27 to day 29 0.4 chickens with similar weights were selected from each replicate, 2 of which were injected with physiological saline and 2 with LPS, with an injection dose of 1 mg/kg BW. Three hours after the LPS injection, and then the chickens were killed by cervical dislocation.

All animal procedures used in the present study were approved by the Institutional Animal Care and Use Committee of Wuhan Polytechnic University (Index number: WPU202306002). Broiler chickens are raised in cages, with 10 chickens per cage. Before the start of the experiment, the chicken houses, feed troughs, chicken pens, and water troughs were thoroughly cleaned, and the chicken houses were fumigated and disinfected with potassium permanganate and formaldehyde. During the raising period, the light is maintained continuously, and the overall humidity of the chicken house is controlled at 50–60%. The temperature was controlled at 30–33°C 1 week before the chickens were introduced, then the temperature was reduced by 2–3°C every week, and the temperature was controlled at 24°C until the end of the experiment. During the experiment, the feces should be cleaned regularly and ventilated frequently, and environmental sanitation should be also maintained. The broilers’ mental status and feed intake should be observed and recorded daily.

Lipopolysaccharide (sourced from Escherichia coli 0111: B4, item number L2630), purchased from Sigma Reagent Company in the United States. The freeze-dried L. rhamnosus GG powder contained 3 × 10⁹ colony forming units (CFU)/g and was stored in the sealed packet at 4°C until used. In the experiment, the basic feed was prepared according to the Chinese broiler breeding standard (2004), and the feed composition and nutrition level are shown in Table 1.

Approximately 3 h after the LPS injection, blood was collected from the broiler chickens. Fabricius’s duodenum, jejunum, ileum, liver, thymus, spleen, and bursa were quickly dissected and separated, and their weights were recorded. Each intestine was rinsed with precooled PBS, frozen in liquid nitrogen, and then stored at −80°C for a long period until testing. A sample of about 1 cm from each intestine was fixed in a 4% paraformaldehyde solution and sent to Wuhan Bolfu Biochemical Company for hematoxylin and eosin (H&E) staining sections.

Based on recorded body weight and feed intake, the average daily gain (ADG), average daily feed intake (ADFI), and feed-to-weight ratio (F/G) were calculated.

The collected blood was separated into plasma and stored at −80°C. Concentrations of biochemical parameters in plasma [total bilirubin (TB), total cholesterol (TC), triglyceride (TG), albumin (ALB), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol] were measured with corresponding kits using a Hitachi 7,060 Automatic Biochemical Analyzer. (Hitachi, Japan).

Fabricius’s liver, thymus, spleen, and bursa were weighed and recorded. Organ index (g/kg) = fresh weight of the organ (g)/fasting live weight of the broiler before slaughter (kg).

The diamine oxidase (DAO) amount detection kit was purchased from Nanjing Jiancheng Bioengineering Institute. All procedures were followed by the kit instructions (DAO: Category number A088-1-1).

Fractionated plasma was used to analyze antioxidant and oxidation-related products. The activities of catalase (CAT), total superoxide dismutase (T-SOD), myeloperoxidase (MPO), and the concentration of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) were determined by using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the protocols of the manufacturer.

The intestinal segments were removed from the 4% paraformaldehyde solution and embedded in paraffin, and H&E staining sections were made. Randomly select 5–10 relatively complete intestinal villi from the slice images, and use an OLYMPSBX-41TF optical microscope (BX-41, Olympus, Japan) to observe and measure the villus height (VH) and crypt depth (CD). VH is the vertical distance from the top of the villus to the opening of the intestinal gland; CD is the vertical distance from the crypt opening to the crypt bottom.

Total RNA from intestinal or liver tissue was extracted using TRIzol™ Reagent Kit (Invitrogen, United States). Approximately 0.1 g intestinal or liver tissue in a 2-ml Eppendorf Tube (EP) was added with a 1-ml TRIzol Reagent, then homogenized at 4°C. Total RNA was extracted according to the instructions by the kit’s manufacturer. The RNA concentration and purity of the sample (A260:A280 = 1.7–2.1, A260:A230 > 2.0) were determined using a microspectrophotometer (NanoDrop 2000, Thermo Fisher Scientific, Inc., United States), and all Samples were diluted evenly to the same concentration. The extracted RNA was reverse transcribed into cDNA using PrimeScript^® RT Reagent Kit with gDNA Eraser (TaKaPa, Dalian). SYBR^® Premix Ex TaqTM (TaKaPa, Dalian) on-machine kit was used to detect the expression of genes related to intestinal barrier function, tissue injury, and inflammatory response through a real-time fluorescence quantitative polymerase chain reaction (PCR) instrument (ABI 7500, Thermo Fisher Scientific, United States). β-actin was used as the internal reference gene. The sequences of the primers used in the experiment are shown in Table 2. The primers were synthesized by Shanghai Sangon Bioengineering Co., Ltd. PCR reaction system 10 μL: Tap Pro Universal SYBR qPCR Master Mix 5 μL, cDNA 1 μL (50 ng), RNase-free ddH₂O 3.6 μL, upstream and downstream primers (10 μmol/L) 0.2 μL each. PCR amplification program: predenaturation at 95°C for 30 s; denaturation at 95°C for 5 s, annealing at 60°C for 34 s, a total of 40 cycles; melting curve program: denaturation at 95°C for 15 s, annealing at 60°C for 1 min, and extension at 95°C for 15 s. The relative expression of each gene was calculated using the 2^−ΔΔCt method.

The experimental data were analyzed using Statistical Package for the Social Sciences (SPSS) version 27.0 (SPSS, Inc., Chicago, IL, United States) statistical software. Growth performance data were assessed using one-way analysis of variance (ANOVA) followed by Duncan’s multiple comparisons test. The remaining data were analyzed using a 2 × 2 factorial design followed by Duncan’s multiple comparisons test. Among them, p < 0.05 denotes the difference is significant, and 0.05 ≤ p ≤ 0.10 denotes a trend.

As shown in Table 3, LGG addition to the diet did not have a significant impact on ADG, ADFI, and F/G of broiler chickens.

As listed in Table 4, LPS stimulation increased the ALT activity and decreased the amounts of TC, TG, ALB, ALP, HDL, and LDL in plasma, while LGG increased the amount of LDL and ALT activity (p < 0.05). There was a tendency for LGG to increase the amount of TB (p = 0.071).

As shown in Table 5, LPS and LGG significantly decreased the GSH-Px activity in plasma (p < 0.001). On the contrary, there were tendencies that LPS decreased T-SOD activity (p = 0.065) and H₂O₂ content (p = 0.098).

As shown in Figure 1, LPS (p = 0.036) and LGG (p < 0.001) significantly decreased the amount of DAO in plasma. There were interactive effects on the amount of plasma. Under LPS stimulation, the supplementation of LGG decreased the amount of DAO in plasma (p < 0.001).

As shown in Figure 2, alphabet a: LPS stimulation caused the intestinal villi to fall off or break in broiler chickens, and the integrity of the villi is reduced, while the LGG addition maintained the structural integrity of the villi to a certain extent; alphabets b and c: LPS stimulation significantly decreased VH in duodenum (p < 0.001), CD in ileum (p = 0.015). LGG addition increased VH in the duodenum (p = 0.016), jejunum (p < 0.001) and ileum (p < 0.001), CD in the duodenum (p = 0.035), and ileum (p < 0.001). There were interactive effects on CD in the jejunum and ileum. Compared with broiler chickens stimulated with LPS, LGG addition significantly increased VH (p < 0.001) in the duodenum and CD in the ileum (p = 0.015) and decreased CD in the jejunum (p = 0.002) (Figure 3).

As shown in Table 6, LPS significantly elevated the relative expression of Occludin in the jejunum (p = 0.014) but decreased the relative expression of Mucin2 in the duodenum (p = 0.001). LGG significantly increased the relative expression of Mucin2 in the duodenum (p < 0.001) and Occludin in the jejunum (p < 0.001). There were interactive effects on the relative expression of Occludin in the duodenum and Mucin2 in the jejunum of broiler chickens. Compared with broiler chickens stimulated with LPS, the relative expression of Mucin2 in the jejunum (p < 0.001) of broiler chickens was higher after LGG supplemented.

As shown in Table 7, There is an interaction between LPS stimulation and LGG. Under LPS stimulation, LGG addition can significantly increase the relative weight of the bursa of Fabricius in broilers (p = 0.006).

As listed in Table 8, LPS stimulation significantly elevated the relative expression of IL-8, IL-1β, and TNF-α in the duodenum (p < 0.001), and elevated the relative expression of IL-8, IL-1β, iNOS, and TNF-α in the jejunum (p < 0.001). LGG addition significantly decreased the relative expression of IL-8 (p = 0.038) iNOS (p < 0.001) in the duodenum, and IL-1β (p = 0.027) in the Jejunum; LGG addition significantly increased the relative expression of IL-1β (p = 0.014) and TNF-α (p = 0.010) in the duodenum and IL-8 (p = 0.001), and TNF-α (p < 0.001) in the Jejunum. There is an interaction between LPS stimulation and LGG. Under LPS stimulation, LGG addition can significantly increase the relative expression of TNF-α in the jejunum (p = 0.001).

As shown in Table 9, LPS significantly increased the relative expression of IL-8, IL-1β, iNOS, and TNF-α (p < 0.001) in the liver of broiler chickens.

As listed in Table 9, LPS stimulation significantly decreased the relative expression of BCL2 (p = 0.004), HSP70 (p < 0.001), and MMP13 (p < 0.001) in the liver and increased the relative expression of MMP9 (p < 0.001); LGG addition significantly increased the relative expression of MMP13 (p = 0.003) and MMP9 (p = 0.013). There is an interaction between LGG and LPS stimulation in the relative expression of MMP9. Compared with broiler chickens stimulated with LPS, LGG addition significantly elevated the relative expression of MMP9 in the liver (p = 0.013).

As shown in Table 9, LPS stimulation significantly decreased the relative expression of SOD (p = 0.013) and CAT (p < 0.001) in the liver but significantly elevated the relative expression of GSH1 (p < 0.001) in the liver.

The beneficial effects of LGG in animal production have been confirmed by numerous experiments. Probiotics can effectively promote the absorption of mineral elements, vitamin metabolism, and physiological regulation of the digestive tract, thereby improving the growth performance of animals (Zhang et al., 2024). Studies have shown that LGG addition can significantly decrease the number of E. coli in the intestinal tract of chickens, increase ADG, reduce F/G, and improve growth performance (Chen et al., 2018). However, in this experiment, LGG had no significant effect on the growth performance of broiler chickens, and consistent results were found in the study of Song. Song et al. (2022) found that Lactobacillus plantarum had no significant effect on the growth performance of Daheng broilers. Song et al. (2006) found Miichthysmiiuy different doses of Clostridium butyricum (10³ ~ 10⁹ CFU/g basal diet), and the results showed that the growth performance of the fish was related to the content of C. butyricum. Therefore, we speculate that the lack of significance in the growth performance of this test may be related to the content of LGG.

Blood has the function of transporting nutrients in the animal body, and blood indicators reflect the absorption of nutrients and the health status of the body to a certain extent. TC refers to the sum of cholesterol contained in all lipoproteins in the blood. TG and TC jointly reflect the liver’s ability to metabolize fat and nutritional status. ALB content reflects the transport of substances in the blood, cell osmotic pressure, and the body’s immune status (Zhang et al., 2021). ALT can effectively reflect liver injury; ALP can reflect bile duct damage, and both are important indicators of liver health (Aravind et al., 2003). The high amount of HDL means that the animal body can inhibit the development of diseases such as cancer by affecting immune responses, while lower HDL means that the ability of LDL to oxidize is enhanced, leading to increased intracellular oxidative stress (Ikonen, 2008). Studies have shown that LPS can significantly increase the amount of ALT in serum and significantly decrease the amount of TC, TG, ALB, ALT, HDL, and LDL in serum (Zhang et al., 2021; Huang et al., n.d.). Consistent with the results of this experiment, it shows that LPS may cause liver injury, resulting in a decrease in liver metabolism and nutritional status. The above results indicate that LPS causes liver injury, and LGG has a slight effect on the plasma parameters of broiler chickens.

In livestock and poultry production, oxidative stress is associated with various syndromes. Factors such as environmental, physiological, and pathological influences can easily lead to oxidative stress in the body, resulting in injury to tissue function (Li et al., 2019). The liver is an important central organ for metabolism and detoxification of the body. We have found that LPS caused liver injury and the study shows the liver is also a targeted effector organ in response to oxidative stress (Li et al., 2015). Antioxidant enzymes like T-SOD, GSH-Px, and CAT play a crucial role in scavenging free radicals in the body. The activity of these enzymes directly indicates the body’s antioxidant capacity and helps maintain the balance of free radicals (Kumar et al., 2012). GSH-Px, in particular, is a key peroxide-decomposing enzyme that can effectively eliminate superoxide and hydroxyl free radicals, safeguarding cell membrane structure and function from peroxide-induced damage (Limón-Pacheco and Gonsebatt, 2009). GSH1 encodes glutathione synthase, which is a key enzyme in the glutathione synthesis process. Glutathione has antioxidant effects (Lisowsky, 1993; Grant et al., 1997). SOD is a type of enzyme that can catalyze the dismutation of superoxide anion free radicals (O²⁻) into hydrogen peroxide (H₂O₂). Its main function is to remove superoxide anion free radicals that are harmful to the body. Low activity of enzymes such as SOD and GSH-Px in the blood will break the homeostasis between the production and removal of free radicals in the body (Saltiel and Olefsky, 2017). Studies have shown that LPS can significantly decrease the activities of GSH-Px, CAT, and T-SOD in serum and tissues, causing oxidative injury and liver injury (Huang et al., n.d.; Li et al., 2015; Zheng et al., 2016; Zheng et al., 2020; Han et al., 2020). This result is similar to the results of this experiment. In this experiment, LPS significantly decreased GSH-Px activity in plasma and the relative expression of CAT and SOD in the liver and had a tendency to reduce T-SOD activity in plasma. The above results indicate that LPS causes oxidative stress in the body. MA studies have found that LGG addition can increase the GSH-Px activity in the kidney and alleviate renal lipid peroxidation caused by DON (Ma et al., 2022). However, the results of this experiment show that LGG addition will significantly reduce the activity of GSH-Px in serum, which may be related to the complex redox reactions of the body. More relevant research is needed to explain it.

The small intestine is the main place where animals digest and absorb nutrients. The mechanical barrier is an important component of the intestinal mucosal barrier, and the tight junctions between cells and the underlying adherens junctions and desmosomes play an important role in maintaining the integrity of the intestinal mechanical barrier (Yao et al., 2022). DAO is a biomarker related to intestinal permeability, and its serum level can reflect the integrity of the intestinal barrier. DAO mainly exists in intestinal epithelial cells, and a complete intestinal mucosal barrier can prevent D-lactic acid and DAO from entering the portal circulation. When the intestinal barrier is damaged, it can enter the blood circulation system through the intestinal mucosa, resulting in increased DAO activity in the plasma (Ji et al., 2018; Fukudome et al., 2014; Xue et al., 2018). LPS not only changes the normal intestinal morphology but also damages the intestinal mechanical barrier. Studies have shown that LPS treatment will reduce the expression of tight junction proteins Occludin and ZO-1 in the ileum of piglets and increase the activity of DAO in the serum of piglets (Sun et al., 2021). Deng’s research found that Lactobacillus casei can significantly reduce the secretion of DAO in the intestinal tract of chickens, improve intestinal permeability, and protect the intestinal mucosal barrier function (Deng et al., 2021). In this experiment, LGG addition significantly decreased the amount of DAO, and the same results were obtained under LPS stimulation, which is consistent with Deng’s results and further illustrates that LGG may have the function of protecting the intestinal barrier.

Digestion and absorption are the most basic functions of the intestine. Different types of animals and different parts of the digestive tract have different digestion methods. The digestion and absorption functions of poultry are mostly concentrated in the small intestine (Wu et al., 2013). Whether the intestinal morphology is normal or not can reflect the digestion and absorption function of the animal’s intestines. Studies have shown that LPS can cause intestinal morphological damage, such as mucosal edema, bleeding, and even necrosis (Ma et al., 2015). Previous studies have shown that LPS stimulation destroyed the intestinal structure of broiler chickens, which is manifested by the decrease of VH and the increase of CD (Li et al., 2015; Hu et al., 2011). Consistent with the above results, in this experiment, LPS significantly decreased the VH of the duodenum. Studies have found that oral administration of various probiotic lactic acid bacteria, such as L. rhamnosus and Lactobacillus reuteri can significantly increase the VH (Ciorba et al., 2012). Mao et al. found that L. rhamnosus significantly increased the VH in the jejunal mucosa of weaned piglets (Mao et al., 2016). Consistent with this experiment, in this experiment, LGG addition significantly increased the VH in the duodenum, jejunum and ileum. Under LPS stimulation, LGG addition significantly increased the VH in the duodenum. In summary, it was found that LGG has the ability to alleviate the injury to the intestinal morphological structure caused by LPS, improve the digestive and absorption function of the small intestine, and protect the integrity of intestinal mucosal tissue morphology.

The tight junctions of intestinal epithelial cells are the prerequisite for ensuring the structural integrity of the intestinal mucosal barrier. Occludin is a transmembrane protein whose main function is to maintain the tight junction structure and protect the intestinal barrier (Aravind et al., 2003; Bewley et al., 2013). The mucus secreted by intestinal mucosal epithelial cells is also one of the important tissue parts of the intestinal mucosal barrier (Johansson et al., 2013). The main component of mucus is mucin, of which Mucin2 is one of the most abundant mucins secreted by goblet cells (Kurashima and Kiyono, 2017). It can form a protective mucus layer and dynamically interact with intestinal epithelial cells, microbiota, and host immune defense to maintain intestinal Mucosal homeostasis (Martens et al., 2018; Castagliuolo et al., 1996). Therefore, changes in Mucin2 are related to a variety of intestinal diseases and are an important factor in expressing the status of the intestinal mucosa. LPS stimulation reduced the expression of Mucin-2 and Occludin in the intestinal tract of broiler chickens (Cui et al., 2024; Yang et al., 2019). In this experiment, LPS significantly decreased the expression of Mucin-2 in the duodenum, consistent with the above results, but LPS significantly increased the expression of Occludin in the jejunum. It is speculated that it may be because LPS activates the autoimmune regulatory function of broilers and increases the expression of tight junction proteins to resist the injury of LPS to the intestines. Combined with the intestinal morphology data, it was considered that LPS stimulation caused damage to the intestinal barrier. Studies by Patricia WLin and others have shown that LGG can regulate the expression of genes related to intestinal epithelial cell proliferation, differentiation, and migration, induce the expression of antiapoptotic genes, maintain the stability of epithelial cells, and activate protein kinase C and mitogen-activated protein kinase pathways to promote Occludin expression in intestinal epithelial cells (Lin et al., 2008; Patel et al., 2012; Shaffiey et al., 2016). Studies have pointed out that probiotics, in addition to chickens, can significantly increase the gene expression of Occludin and Mucin2 in the ileum (Chang et al., 2020). The results of the present experiment were consistent with the above studies. In this experiment, LGG addition significantly increased the relative expression of Mucin-2 in the duodenum and significantly increased the relative expression of Occludin in the jejunum. Under LPS stimulation, LGG addition significantly increased the relative expression of Mucin-2 in the jejunum. Combining the present results that LGG decreased DAO amount in plasma and improved injury intestinal morphology, it has shown that LGG alleviated LPS-induced intestinal damage.

The strength of the animal’s immune function and the body’s nutritional health status can be reflected by organ indexes. The spleen, thymus, and bursa of Fabricius are the main organs for immune regulation in the body (Nakamura et al., 2012; Rivas and Fabricant, 1988). The bursa of Fabricius is a central immune organ unique to birds, participating in humoral immune responses and improving resistance. Chen et al.’s study showed that 1.5 g/kg of xylo-oligosaccharide (100 mg/g), C. butyricum (2 × 10⁹ CFU/g), Bacillus subtilis (3×10¹⁰ CFU/g) and attapulgite Synbiotics addition can significantly increase the bursa index and intestinal immunoglobulin content of Cherry Valley ducks, suggesting that synbiotics can significantly promote the development of immune organs in Cherry Valley ducks and improve the body’s immune function (Chen et al., 2018). In this experiment, under the stimulation of LPS, LGG addition can significantly increase the relative weight of the bursa of Fabricius, which is consistent with the above results, suggesting that LGG can promote the development of immune organs in broiler chickens.

In the intestine, the homeostasis of the intestinal immune system affects intestinal and body health. Cytokines are a type of soluble small peptides secreted by immune cells and tissue cells that regulate each other between cells. IL-1β and IL-8 all belong to cells Factors can regulate the body’s immune response through receptors and play an important role in mediating trauma, infection, autoimmune diseases, and inflammatory reactions (Zhang et al., 2020). IL-8 is an important member of the chemokine family. It is secreted by macrophages and other cells. It can mediate neutrophils to participate in the body’s inflammatory response and is an important proinflammatory factor. It induces the body to produce inflammatory mediators and promote a large number of inflammatory factors (TNF-α and IL-8) (Hsieh et al., 2018; Ren et al., 2021). iNOS is mainly expressed in giant cells and leukocytes. In inflammatory reactions, iNOS promotes the release of NO (Hashemi Goradel et al., 2019; Anavi and Tirosh, 2020; Lopes et al., 2022). LPS significantly decreases the expression of Occludin and Claudin-1 and mediates the expression of IL-1β, IL-8, and TNF-α inflammatory cytokines through the NF-κB and Nrf2 signaling pathways. The expression of inflammatory mediators can mediate iNOS, and excessive production of NO induces oxidative stress, accelerates inflammatory reactions, and leads to intestinal injury (Zhang et al., 2020; Song et al., 2016; Xu et al., 2023; Han et al., 2017). In this experiment, LPS significantly increased the relative expression of IL-8, IL-1β, and TNF-α in the duodenum and IL-1β, IL-8, iNOS, and TNF-α in the jejunum and liver. The results are the same as the above, indicating that we caused an inflammatory response in the intestine through LPS, and the establishment of this intestinal injury model was successful. A study by Li et al. demonstrated that L. rhamnosus GR-1 can induce PINK1/Parkin to mediate mitochondrial autoregulation, clear damaged mitochondria and decrease ROS production and NLRP3 inflammasome activation, decrease E. coli-induced apoptosis, and can decrease the levels of IL-1β, IL-8, TNF-α and other cytokines (Li et al., 2021). Fukuyama et al. found that Lactobacillus acidophilus CRL2074 and L. rhamnosus CRL2084 had a better regulatory effect, decreasing the relative expression of IL-1β, IL-8, and CXCL3 (Fukuyama et al., 2020). In this experiment, LGG addition significantly decreased the relative expression of IL-8 in the duodenum and IL-1β in the jejunum. The results of this trial are similar to the above results, indicating that LGG has the potential to enhance intestinal immunity.

The normal maintenance of the body’s immune function is conducive to improving the intestinal barrier function and the body’s resistance to diseases and infections. Based on previous research findings, LGG has a critical protective effect on the intestinal barrier and intestinal immune function, and then its effect on the liver was studied. The liver is the body’s most important metabolic and innate immune organ. Its abundant natural killer cells and macrophages are the main line of defense for natural immunity in the liver (Zhi et al., 2020). Most inflammatory responses are related to immune mechanisms, and macrophages are the key factor in initiating inflammation (Cho et al., 2021). Heat shock proteins are a type of highly conserved stress proteins. Hsp70 is the most highly conserved protein known so far. It has a protective effect on cells under stress conditions (such as ultraviolet radiation, bacterial infection, or inflammation), such as improving cell Injury tolerance and stress capacity (Jeyachandran et al., 2023). Lyu et al. found that LPS stimulation down-regulates Hsp70 and up-regulates the expression of proinflammatory mediators TNF-α and IL-6 induced by it, thereby inhibiting the activation of NF-κB in macrophages (Lyu et al., 2020). In this experiment, LPS caused oxidative stress and liver damage and significantly decreased the relative expression of Hsp70 in the liver, indicating that LPS caused stress injury to broilers. Matrix metalloproteinase 9 (MMP9) is an important pathogenic factor in inflammatory diseases. High concentrations of MMP9 can lead to increased intestinal tight junction permeability in vitro or in vivo (Al-Sadi et al., 2019). Experiments have shown that the expression of MMP9 in the damaged liver increases significantly when acute liver failure is induced by LPS, etc.; in addition, MMP9 also plays a certain role in acute hepatitis (Yan et al., 2008; Wielockx et al., 2001). In this experiment, LPS significantly increased the relative expression of MMP9 in the liver. Escamilla et al. (2012) used LGG to reduce the infiltration, invasion, and metastasis capabilities of tumor cells by reducing the expression of MMP9, inhibiting the degradation of IκB, and increasing the expression levels of IL-8 and ZO-1 (Escamilla et al., 2012). Consistent with the above results, in this experiment, LGG addition significantly decreased the relative expression of MMP9 in the liver; under LPS stimulation, LGG addition significantly decreased the relative expression of MMP9 in the liver, indicating that LGG may have a liver protective effect.