AUTHOR=Li Yang , Luo Juan , Zhang Fuyou , Shang Jiajing , Deng Chunran , Feng Yingjie , Meng Ge , Jiang Wenming , Yu Xiaohui , Liu Guanhui , Liu Hualei 

TITLE=Establishment and application of quadruple fluorescence quantitative RT-PCR method for the identification of waterfowl astrovirus

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1328243

DOI=10.3389/fmicb.2024.1328243

ISSN=1664-302X

ABSTRACT=Avian astroviruses can infect a wide range of avian species and cause viral diarrhoea, which is widespread, highly pathogenic and has a high incidence rate, causing a certain impact on the healthy development of China's waterfowl aquaculture industry. In order to achieve rapid detection of DAstV-3, DAstV-4, GoAstV-1 and GoAstV-2, a multiplex fluorescent RT-PCR method was developed in this study. DAstV-3, DAstV-4, GoAstV-1 and GoAstV-2 can be detected simultaneously in one reaction tube, which consumes short time and has good specificity. Compared with the routinely used RT-PCR method, the limit of detection by the multiplex RT-PCR lower. Subsequently, 269 clinical samples were tested by this method and the results showed that the positivity rate of DAstV-4 was high, while the positivity rates of DAstV-3, GoAstV-1 and GoAstV-2 were low, and mixed infections existed between DAstV-4 and GoAstV-1 and GoAstV-2.