Microorganisms possess several mechanisms to acclimatize to stress conditions that influence growth and survival in saline environments. Halophiles are microbes that can survive such saline conditions from low to high saturation points. There are different stress proteins and strategies that halophiles adapt to counteract stressful factors such as ions, temperature, pH, and UV radiation. Prokaryotic halophiles have attracted the attention of researchers worldwide because of their distinctive features, ease of manipulation, lesser space requirements for cultivation, and the production of diverse metabolites compared to plants or eukaryotic algae (Torregrosa-Crespo et al., 2017; Dutta and Bandopadhyay, 2022). A significant group of halophilic archaea, represented under the halobacteria class, are tolerant to extreme saline environments. These environments include salt lakes, estuaries, rivers, mangrove swamps, open seawater, coastal waters, salt lakes, estuaries, and salt deserts. Halobacterial class constitutes a wide range of genera (Figure 1) – Salarchaeum, Halobiforma, Natronolimnobius, Halopelagius, Halogranum, Halonotius, Haladaptatus, Natronococcus, Haloferax, Halococcus, Haloalcalophilium, Halorubrum, Halorhabdus, Halorussus, Halopiger, Halomarina, Natronoarchaeum, Halobellus, Natrialba Halobaculum, Haloplanus, Halostagnicola, Halorientalis, Natronomonas, Natrialba, Natronobacterium, Natronorubrum, Haloarcula, Halobacterium, Haloterrigena, Natrinema, Halogeometricum, Halalkalicoccus, Haloquadratum, Halogeometricum, Natronorubrum, Halomicrobium, Halolamina, Halovivax, Halarchaeum, and Halosimplex (Cui and Dyall-Smith, 2021).

Considering the difficulties found by researchers to obtain pure cultures of extremophilic microorganisms (including halophilic archaea) from environmental samples, and consequently to know the microbial biodiversity in those samples, recent advances in omic-based approaches have contributed to a better understanding of haloarchaeal biodiversity. Particularly, metagenomic analysis has been used to overcome this limitation. Metagenomic analysis of environmental samples from the Dead Sea, RedSea, Gulf of Cambay, Mediterranean Sea, Sundarbans mangrove forest, Karak Salt Mine, and Pannonian Steppe, revealed the predominance of haloarchaeal genera in the natural environment, including Haloarcula, Halorubrum, and Halorhabdus (Oh et al., 2010; Rhodes et al., 2012; Somboonna et al., 2012; Fernández et al., 2014; Bhattacharyya et al., 2015; Keshri et al., 2015; Behzad et al., 2016; Szabó et al., 2017; Haldar and Nazareth, 2018; Osman et al., 2019; Cecil et al., 2020). Similarly, a global metagenomic meta-analysis revealed the dominance of Haloarcula and Haloquandratum sp., in the seacoast (Moopantakath et al., 2021). The biogeography of haloarchaea also varies based on the biosystems. For instance, the Haloferax genus is highly dominant in seashores and island samples and estuaries, while the Natrialba genus is predominant in rivers, mangroves, and lakes (Hegazy et al., 2020; Moopantakath et al., 2020; Cho et al., 2021). Thus, the prevalence of halophilic microorganisms in general, and particularly of haloarchaea, is highly dependent on the environment, geography, and physicochemical parameters like salinity, pH, oxygen availability and sun radiation, among other factors (Ventosa, 2006; Mani et al., 2020). As an example, recent whole genome sequencing studies revealed that autotrophic haloarchaea are highly abundant in coastal environments, probably due to salinity and direct sunlight exposure (Moopantakath et al., 2021). These environments display unique features and salt deposition phenomena that contribute to the increase of some haloarchaeal populations requiring extremely high concentrations for optimal growth.

Environmental degradation, such as the release of industrial chemicals into the coastal environment, is also a major factor in pollution and shoreline contamination (Lu et al., 2018) and in microbial biodiversity. Nonetheless, the microbial community plays a significant role in the polluted site, and the unique metabolic features of haloarchaea contribute to the homeostasis of these environments. Halorhabdus and Natrinema sp., have been reported to degrade xylan and produce halocin (antimicrobial peptide), respectively (Begemann et al., 2011; Besse et al., 2017). Similarly, recent advances in the metagenomic analysis have revealed that haloarchaea, such as Haloferax, Haladaptatus, Natrialba, etc., participate in maintaining the biogeochemical processes in coastal ecosystems (Osman et al., 2019). Some haloarchaea can carry out interesting metabolic processes from an industrial point of view; examples: sulfur reduction by Natranaeroarchaeum sulfidigenes (Sorokin et al., 2022), nitrification by Haloarcula, Halolamina, and Halobacterium (Wei et al., 2022), phosphorus solubilization by Haloarcula, Halobacterium, Halococcus, and Haloferax (Yadav et al., 2015) or denitrification by Haloarcula or Haloferax (Miralles-Robledillo et al., 2021).

In summary, haloarchaea exhibit diverse metabolic pathways and biological activities of interest for biotechnological purposes. They constitute predominant microbial communities in salty ecosystems, which are widespread worldwide. For example, Haloarcula, Haloferax, and Halogeometricum sp., can be isolated from saline sediments across the world, such as from Spanish coastal and inland salted ponds (Martínez et al., 2022), Algerian salt lakes, Indian salt pans, Verkhnekamsk deposit etc., and exhibit several biological activities, including the production of carotenoid pigments (Das et al., 2019; Sahli et al., 2022). Besides, many more ecosystems characterized by their high salt concentrations are far from known from a microbiological point of view (i.e., saline mines in Senegal). This review aims to summarize new advances in the knowledge of biological applications of halophilic archaea and the synthesis of secondary metabolites thus contributing to the design of new biotech processes low cost and environmentally friendly based on the use of haloarchaea as cellular factories.

Several biotechnological-based processes can be benefited from the use of haloarchaea. Thus, the use of whole cells in wastewater treatments and bioremediation of brines and salty soils has been recently revealed as a promising tool. Hypersaline wastewater is a common byproduct of industrial processes. Hence, cost-effective treatment of hypersaline wastewater is necessary for sustainable development. Biological wastewater treatment has been considered a more economical approach, but mesophilic microorganisms used so far for this purpose can not be used in the biological treatment of polluted brines of wastewater containing high salt concentration. In this context, recent research findings revealed the role of haloarchaea in the treatment of salty wastewater and brine generated as final residues in water desalination plants (Li et al., 2021; Martínez et al., 2022). Haloarchaea can also degrade hydrocarbons; however, their degradation is more efficient for low molecular-weight hydrocarbons. The degradation of naphthalene, phenol, p-hydroxybenzoic acid, and 3-phenyl propionic acid, and oxychlorides like perchlorate or chlorate by haloarchaea also make it an attractive choice for wastewater treatment (Martínez-Espinosa et al., 2015; Mukherji et al., 2020; Li et al., 2021). Another interesting approach related to wastewater treatments is the removal of nitrogen to avoid eutrophication in the receiving water bodies and heavy metals to avoid global toxicity. Haloarchaea, such as Haloferax mediterranei, can use NO₃^– and NO₂^– as nitrogen sources for growth or as final electron acceptors instead of oxygen in an anaerobic respiratory process (denitrification). For example, Haloferax mediterranei encodes nitrate reductase (nas) and nitrite reductase (nir) that can perform assimilatory nitrate/nitrite reduction (Martínez-Espinosa et al., 2007), whilst Nar, NirK, Nor, and Nos encodes key enzymes in catalyzing the reactions involved in the process of denitrification (Bernabeu et al., 2021). Regarding heavy metals, several recent studies have demonstrated that some haloarchaeal species can grow in the presence of heavy metals at concentrations that are toxic for most living beings. In some cases, because of cellular growth, heavy metals are accumulated, modified or bioassimilated. Molecular machinery for the potential removal of cooper and cadmium has also been identified from many haloarchaeal species (Vera-Bernal and Martínez-Espinosa, 2021; Llorca and Martínez-Espinosa, 2022). In other cases, haloarchaea growing in the presence of heavy metals can also synthesize nanoparticles (NPs) from wastewater polluted with cadmium, arsenic, and zinc (Taran et al., 2017; Li et al., 2021; Gaonkar and Furtado, 2022). Haloarchaea have various mechanisms to tolerate the arsenate metals using minichromosomes/megaplasmids (arsADRC gene cluster) in the Halobacterium species (Wang et al., 2004; Voica et al., 2016). The zinc tolerance mechanism was found due to the presence of physical bioabsorption, ion exchange and intracellular accumulation which can be used for the various biological process inside the cell (Popescu and Dumitru, 2009; Williams et al., 2013; Salgaonkar et al., 2016). Similarly, cadmium also plays a crucial aid in intracellular metabolic functions so cadmium can be tolerated inside the cell without stress (Vera-Bernal and Martínez-Espinosa, 2021).

Another interesting biotechnological application is related to the enzymes from haloarchaea. They exhibit increased tolerance, not only to salinity but also to pH, pressure, temperature, etc. Haloenzymes have several advantages, such as minimum steps in purification, sterilization, and cost-effectiveness (Amoozegar et al., 2017). Haloenzymes such as lipase and alcohol dehydrogenase from Haloferax volcanii and Haloarcula sp., G41, respectively, have been immobilized successfully for increased activity (Alsafadi and Paradisi, 2014; Li and Yu, 2014). Saline-tolerant lipases and esterases like those described from Haloarcula marismortui and Natronococcus sp., TC6 are essential enzymes in several biotechnological applications such as biofuel, detergent, textile, etc (Camacho et al., 2009; Del Campo et al., 2015).

The haloarchaeal membrane protein bacteriorhodopsin, initially discovered from Halobacterium salinarum, is highly stable to thermal and photochemical stress. Bacteriorhodopsin can sense light and convert it into electrical signals (Singh and Singh, 2017). Bacteriorhodopsin from haloarchaea has also found applications in biosensors and artificial retinas (Figure 2; Amoozegar et al., 2017). The gas vesicles mentioned in section “1. Introduction: haloarchaeal diversity and ecology” have applications in drug delivery systems and vaccine development (Cánovas et al., 2021). Meanwhile, poly-β-hydroxy-alkanoates (PHAs) produced by haloarchaea, such as Haloferax mediterranei, are considered an alternative to plastics produced from petroleum and in medical applications owing to their biocompatibility (Salgaonkar et al., 2013; Li et al., 2021). Thus, PHA can be synthesized using these microorganisms as biofactories thanks to cheaper procedures in which the biomass downstream process as well as the purification process of the biopolymer can be done with single steps (Simó-Cabrera et al., 2021). PHA produced by various materials such as residues from food and agriculture products can be synthesized into PHA with a help of haloarchaea (Quillaguamán et al., 2010). Genomic insights of halophiles such as Halonotius terrestris sp., nov. and Halonotius roseus sp., nov. have been recently delineated and found to encode the complete biosynthetic genes for the biosynthesis of cobalamin (vitamin B12) (Durán-Viseras et al., 2019).

Halophiles produce saline-tolerant proteins and metabolites, such as carotenoids, which assist in tolerance toward salinity. Haloarchaeal carotenoids have industrial interest due to their antioxidant, anticancer, antimicrobial, anti-inflammatory, food colorant, and several other biomedical applications (Verma et al., 2020). Bacterioruberin is also commonly used in cosmetics and drug encapsulation (Serrano et al., 2022). Haloarchaea have been extensively explored for bioactive metabolites with anticancer, antimicrobial, and antioxidant activities (Table 1). Among the several haloarchaeal secondary metabolites, carotenoids gained attention due to their multi-faced applications in cosmetics, food, and biomedical sectors (Serrano et al., 2022). Carotenoids have multiple roles in bacteria, plants, and archaea. Carotenoid helps photosynthetic plants and microbes to increase light absorption in the blue-green region through the singlet-singlet energy transfer (Hashimoto et al., 2015). It also has photoprotective effects against excessive light and reactive oxygen species through the triplet energy transfer of chlorophylls to carotenoids (Maoka, 2020). They are crucial in structural stabilization and render tolerance to hypersalinity.

Due to the advancements in culture-based methods and metagenomics, the identification and discovery of novel species of haloarchaea become a continuous process. Recently, 12 haloarchaeal isolates were isolated from Tamil Nadu (India), out of which nine isolates were novel. Interestingly, all of the isolates produced carotenoids (Verma et al., 2020). Haloarchaea has an inherent molecule known as bacterioruberin, which is found in most haloarchaea (Nagar et al., 2022). Similarly, novel species under the genus Halorubrum, isolated from South Korea, produced C₅₀ carotenoid (bacterioruberin), having a strong antioxidant activity (Hwang et al., 2022). Likewise, carotenoids from novel Haloarcula sp., and Halorubrum sp., strains were isolated from the Atacama Desert (Lizama et al., 2021). Large-scale whole genome (n = 68) analysis suggested that haloarchaea has a wide diversity of carotenoid biosynthetic genes (Serrano et al., 2022), suggesting that haloarchaeal species could be a reservoir of carotenoid derivatives.

The wide range of enzymes produced by halophiles plays a vital role in biotechnology, including biosynthesis, food processing industries, and bioremediation methods. As the enzymes produced by haloarchaea are stable at high salt concentrations they can be utilized in several processes related to food or leather tanning. The most important hydrolytic enzymes produced by haloarchaea are proteases and lipases (Modern et al., 2000). The first hydrolase enzyme, a serine protease, was purified and studied from Halobacterium salinarum. This enzyme was active only at a high concentration of NaCl (more than 2 M), being an enzyme rich in negatively charged amino acids (Ventosa et al., 2005). Starch-degrading alpha-amylases are synthesized by halophiles such as Halobacterium salinarum, Haloferax mediterranei, Halomonas meridiana, and Natronococcus amylolyticus (Kumar et al., 2016). Besides amylases, pullulanase is produced by different archaea, such as Halorubrum sp., Ha25, which is already used in the starch industry (Siroosi et al., 2014). Cellulase-degrading cellulases and glycoside hydrolase gene homologs are present in Halorhabdus utahensis (Zhang et al., 2011), Haloarcula sp., (Ogan et al., 2012), Halorubrum lacusprofundi (Karan et al., 2013), Haloarcula vallismortis (Nercessian et al., 2015), Natronobiforma cellulositropha (Sorokin et al., 2018), Halalkalicoccus jeotgali (Anderson et al., 2011), and Haloferax sulfurifontis (Malik and Furtado, 2019). Table 2 displays different intracellular or extracellular hydrolytic enzymes produced by haloarchaea.

Amylase, one of the important industrial enzymes, was reported to synthesize by a new haloarchaeal strain isolated from salterns. High amylase activity was exhibited by the new strain Haloarcula sp., HS and found to be poly extremotolerant. The optimum enzyme yield was obtained at high salt concentrations (25%), 60°C, and was calcium-dependent. Amylases were found to be synthesized in extracellular and intracellular fractions and observed as 3 different types of enzymes. The extracted enzymes were tested on bakery waste. It was found that amylases degraded bakery waste efficiently at high salt concentrations (Gómez-Villegas et al., 2021). Interestingly, extracellular hydrolytic enzymes were produced by haloarchaeal strains obtained from hypersaline lakes. The two most abundant archaeal species, Natrinema and Halorubrum, produced cellulase, pectinase, amylase, lipase, and xylanase but not protease (Karray et al., 2018). In the study, alpha-amylase obtained from H. salinarum was immobilized in calcium alginate to enhance its stability (Patel et al., 1996). In another study, the tolerance of alcohol dehydrogenase enzyme, obtained from Haloferax volcanii, to organic solvents was reported to improve upon immobilization with sepabeads (Alsafadi and Paradisi, 2014). Immobilized lipase enzyme on anionic resin obtained from Haloarcula sp., was used to produce biodiesel (Li and Yu, 2014). Halobacterium salinarum NRC-1, Haloarcula japonica, H. salinarum CECT 395, and H. mediterranei can grow on chitin thanks to chitinases (García-Fraga et al., 2014; Hou et al., 2014). Haloarchaea degrades lignin through laccase and peroxidase enzymes. Some haloarchaea produces enzymes like esterases and lipases to degrade ester, ether, and glycosidic linkages. Menasria et al. (2018) have reported extensive bioprospecting of salt-stable and active hydrolytic enzymes from haloarchaea of arid and semi-arid wetlands. The major haloarchaea identified were from the class halobacteria such as Haloarcula, Halogeometricum, Halococcus, Haloterrigena, etc. Among the 68 isolates screened, 89.7% of isolates produced 2 halophilic enzymes, whereas 52.9% produced 3 hydrolytic enzymes. These isolates produced gelatinase, cellulase, esterase, and inulinase. Secondly, some isolates were profound in producing xylanase, pectinase, and nuclease. The study also reported that the high cellulase activity (35%) makes it a potential candidate in the food and textile industries (Menasria et al., 2018). Likewise, out of 300 isolates from a salt lake, 293 haloarchaea isolates were selected and studied for active hydrophilic hydrolytic enzymes.

The cellulase, xylanase, amylase, DNase, lipase, protease, pullulanase, chitinase, and inulinase were observed in 9 potential isolates. The most abundant enzymes produced by haloarchaeal isolates (Halorubrum and Haloarcula) were lipase, DNase, and amylase (Makhdoumi Kakhki et al., 2011).

Search for eco-friendly biopolymers is one of the important research objectives worldwide to reduce global plastic pollution and in the production of biomedical devices (Simó-Cabrera et al., 2021). Regarding biomedicine, the desirable properties of biodegradable polymers vary based on their application, such as the high degradative potential for surgical mesh and low degradative potential for bioengineered skin. For such applications, biopolymers can be synthetically prepared, such as polylactic acid (PLA) and polyglycolic acid (PGA) (Han et al., 2015). Yet such synthetically prepared biopolymers are associated with setbacks such as biocompatibility and inflammation. Among natural biopolymers with interesting physicochemical properties to be used as bioplastic for packaging or biomedical applications, polyhydroxyalkanoates (PHAs) from haloarchaea are of research interest. The advantages of PHAs include their ease of mechanical customization, biodegradability, and biocompatibility (Han et al., 2015). Hence, PHAs have been under extensive research for application in medical implants, drug delivery, tissue replacement, etc. PHAs, composed of hydroxyalkanoate monomers, are stored as a source of carbon and energy under stress conditions in archaea and bacteria. The type of hydroxyalkanoate monomers determines the physical properties (rigid or elastic) of the PHAs. Among the PHAs, the most common ones are poly-3-hydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV). PHBHV comprises PHB and 3-hydroxyvalerate (3HV) monomer. PHBHV, upon degradation in the body, does not release toxic byproducts, has more outstanding biocompatibility and biodegradation, and helps in the growth of fibroblasts, mesenchymal stem cells, etc (Ahmed et al., 2010).

Archaea are considered promising cell factories and more cost-effective than bacteria for PHBHV production. For instance, Haloferax mediterranei produces PHBHV with a lower melting point than Hydrogenophaga pseudoflava (Koller et al., 2007). PHBHV produced by Halogranum amylolyticum has a higher hemocompatibility than Ralstonia eutropha (Zhao Y. et al., 2015). Furthermore, the entire process of biosynthesis is within the cell. PHAs are water-insoluble, degradable without oxygen, and increase their solubility in chlorinated solvents (Simó-Cabrera et al., 2021). Based on the monomers, PHAs are classified as homopolymers consisting of the same type of monomers (P4HB, P3HP, P3H4P, etc.), random copolymers consisting of multiple monomer types distributed random, and block copolymers where the distinct polymers are distributed in discrete blocks (Simó-Cabrera et al., 2021). Haloferax mediterranei can also produce varying types of PHBHV based on the concentration of valerate being fed (Han et al., 2015). These variations are of two main types, higher-order copolymers (O-PHBHV) composed of PHB and PHV with random PHBHV segments. R-PHBHV comprises random copolymers 3HB and 3HV (Han et al., 2015). PHAs are also produced by Halogranum amylolyticum TNN58 with poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and 3-hydroxyvalerate (3HV) fraction when the carbon source is glucose (Zhao Y. X. et al., 2015). Genetic engineering, such as CRISPR-Cas technology, can enhance gene expression for PHAs production. A recent study showed a ∼165% increase in the output of PHA when citZ and gltA genes were downregulated by CRISPRi (Lin et al., 2021). Recently, robust methods have been developed for monitoring PHA granules in Haloferax mediterranei, such as through confocal fluorescence microscopy stained with Nile red and SYBR Green (Cánovas et al., 2021).

Nanobiotechnology is a boon to the field of medicine, which deals with the synthesis and application of a wide range of NPs for the treatment of diseases and targeted delivery of drugs (Patra et al., 2018; Dash et al., 2020). Synthesis of NPs using biological entities is always important due to their ease of production, eco-friendly approach, and bioavailability. The green approach in nanotechnology involves producing stable NPs capped with metabolites employed by organisms or plants (Singh et al., 2018). Several works have demonstrated that some haloarchaea can synthesize nanoparticles. However, the synthesis of NPs using haloarchaea needs to be better explored.

Recently, haloarchaea has been explored due to its ability to produce several NPs by detoxifying heavy metals. These organisms survive in the presence of heavy metals by employing enzymatic reduction of metals and sequestration methods to detoxify them (Voica et al., 2016). Table 3 shows different NPs synthesized using haloarchaea as well as their potential biological applications (Table 3). Additional factors such as salt, pH, temperature, and size affect the stability and efficiency of NPs (Dutta and Bandopadhyay, 2022).

Abdollahnia et al. (2020) studied the synthesis of silver and selenium nanoparticles by haloarchaea isolated from solar salterns. Intracellular production of silver and selenium NPs were reported by Haloferax sp., and Halogeometricum sp., respectively (Abdollahnia et al., 2020; Nagar et al., 2022). The biosynthesized nanoparticles exhibited antibacterial activity against S. aureus, E. coli, B. subtilis, and P. aeruginosa (Abdollahnia et al., 2020). A haloarchaea, Haloferax sp., NRS1 screened from solar saltern found in Saudi Arabia showed promising potential in synthesizing silver NPs. The biogenic silver NPs showed non-hemolytic activity below 12.5 μg/ml concentration suggesting their thrombolysis property. The non-toxic/hemolytic property of synthesized NPs potentiates their application as nano drug carriers (Tag et al., 2021).

Similarly, silver NPs synthesized by another group exhibited broad antimicrobial activity. The archaea Halococcus salifodinae BK6, mediated synthesis of silver NPs, employs NADH-dependent nitro reductases to reduce metal to nanoparticles. They showed promising antibacterial activity against S. aureus and M. luteus (Gram-positive) and E. coli and P. aeruginosa (Gram-negative) (Srivastava et al., 2014b).

Srivastava et al. (2014a) discussed the synthesis of selenium NPs using H. salifodinae BK18. Similar to their previous work, nitro reductases reduced sodium selenite to NPs. The intracellular synthesized NPs exhibited antiproliferative properties against HeLa cancer cell lines. Also, the NPs were found to be non-toxic against normal cells suggesting their application as an anticancer agent (Srivastava et al., 2014a). Bioactive gold and silver NPs were synthesized using Haloferax volcanii. Significant antibacterial activities were observed with synthesized silver NPs against E. coli and P. putida (Costa et al., 2020). In addition, haloarchaeal nanoparticles hold promising antibacterial properties. Haloferax alexandrinus was used to synthesize silver nanoparticles with an average size of 18 nm and with an amide carbonyl group on the surface. This synthesized nanoparticle has shown antimicrobial activity against P. aeruginosa, Bordetella bronchiseptica, and S. aureus at 5 μg concentrations (Patil et al., 2014). Another study also highlights the synthesis of spherical-shaped silver chloride nanoparticles (MY6-NP and AK2-NPs) with a size of 30–50 nm using Haloferax Alexandrinus and Haloferax lucentense at high salt-saturated conditions. According to a study by Salem et al. (2020), hyperthermia therapy can be employed with the help of superparamagnetic iron oxide nanoparticles synthesized using Halobiforma sp., N1. The nanoparticles were monodispersed and presented growth inhibition activity against P. aeruginosa PAO1 and Bacillus sp., at 200 μg/ml concentration (Moopantakath et al., 2022).

Haloarchaea, or extremely halophilic archaea, are a group of microbes with genuine metabolic features. They inhabit and even predominate in various extreme geographical and ecological/environmental conditions, The number of new haloarchaeon discovered through culturable and non-culturable methods and techniques is increasing during the last decades. Metabolites synthesized by several haloarchaeal species are of high interest due to their potential applications in biotechnology Thus, carotenoids are highly efficient as antimicrobials, antioxidants, and food colorants. They have been reported to have diverse biological activities, including anticancer and antimicrobial activities. Among carotenoids, bacterioruberin, carotenoids almost produce by haloarchaea show higher antioxidant activity than most of the referenced carotenoids from plants, yeast or algae. Haloarchaea are also implemented in hypersaline wastewater treatment to degrade hydrocarbons, nitrogen removal, and heavy metal bioremediation and nanoparticle (NP) biosynthesis. On the other hand, enzymes from haloarchaea like amylase, chitinase, lipase, protease, and esterase show high activity at extreme conditions (in terms of pH, temperature etc.) which are of interest for industrial processes. The biosynthesis of nanoparticles by haloarchaea has also described being many of those nanoparticles effective against drug-resistant microbes. The haloarchaea, haloenzymes, and pigments are also widely used in the fermentation of salty foods, cosmetics, food, biomedical sectors, biocompatible bioplastics, and biosensors. Despite such an array of applications, their roles within the cells in natural environments as well as in industrial processes remain unexplored consequently, the research on the metabolites synthesized by these microorganisms must continue through more specialized approaches using high-tech equipments.

RK and SB designed the study. JM and VA prepared the figures. RK, SB, MI, JM, and VA wrote the manuscript. RK, SB, RM-E, and MD critically revised the manuscript. All authors have read and agreed to the submitted version of the manuscript.