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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Mater.</journal-id>
<journal-title>Frontiers in Materials</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Mater.</abbrev-journal-title>
<issn pub-type="epub">2296-8016</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="publisher-id">1625770</article-id>
<article-id pub-id-type="doi">10.3389/fmats.2025.1625770</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Materials</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Testing the mechanical performance of a single taper-retained implant&#x2013;abutment connection under static and dynamic loads: an <italic>in vitro</italic> study</article-title>
<alt-title alt-title-type="left-running-head">Wu et al.</alt-title>
<alt-title alt-title-type="right-running-head">
<ext-link ext-link-type="uri" xlink:href="https://doi.org/10.3389/fmats.2025.1625770">10.3389/fmats.2025.1625770</ext-link>
</alt-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Wu</surname>
<given-names>Hao</given-names>
</name>
<role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/>
<role content-type="https://credit.niso.org/contributor-roles/formal-analysis/"/>
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<role content-type="https://credit.niso.org/contributor-roles/conceptualization/"/>
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</contrib>
<contrib contrib-type="author">
<name>
<surname>Cao</surname>
<given-names>Ning</given-names>
</name>
<role content-type="https://credit.niso.org/contributor-roles/data-curation/"/>
<role content-type="https://credit.niso.org/contributor-roles/investigation/"/>
<role content-type="https://credit.niso.org/contributor-roles/Writing - review &#x26; editing/"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Cao</surname>
<given-names>Liang-Wei</given-names>
</name>
<role content-type="https://credit.niso.org/contributor-roles/data-curation/"/>
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</contrib>
<contrib contrib-type="author">
<name>
<surname>Guo</surname>
<given-names>Shui-Gen</given-names>
</name>
<role content-type="https://credit.niso.org/contributor-roles/conceptualization/"/>
<role content-type="https://credit.niso.org/contributor-roles/Writing - review &#x26; editing/"/>
<role content-type="https://credit.niso.org/contributor-roles/methodology/"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Zhang</surname>
<given-names>Xu</given-names>
</name>
<role content-type="https://credit.niso.org/contributor-roles/Writing - review &#x26; editing/"/>
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<role content-type="https://credit.niso.org/contributor-roles/resources/"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Yu</surname>
<given-names>Fei</given-names>
</name>
<role content-type="https://credit.niso.org/contributor-roles/resources/"/>
<role content-type="https://credit.niso.org/contributor-roles/data-curation/"/>
<role content-type="https://credit.niso.org/contributor-roles/Writing - review &#x26; editing/"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Wei</surname>
<given-names>Shi-Bo</given-names>
</name>
<role content-type="https://credit.niso.org/contributor-roles/Writing - review &#x26; editing/"/>
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</contrib>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Wei</surname>
<given-names>Hong-Wu</given-names>
</name>
<xref ref-type="corresp" rid="c001">&#x2a;</xref>
<uri xlink:href="https://loop.frontiersin.org/people/3061901/overview"/>
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<role content-type="https://credit.niso.org/contributor-roles/project-administration/"/>
<role content-type="https://credit.niso.org/contributor-roles/Writing - review &#x26; editing/"/>
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</contrib-group>
<aff>
<institution>Department of Stomatology</institution>, <institution>The Fourth Affiliated Hospital of Nanchang University</institution>, <addr-line>Nanchang</addr-line>, <country>China</country>
</aff>
<author-notes>
<fn fn-type="edited-by">
<p>
<bold>Edited by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/1298652/overview">Giuseppe Rosi</ext-link>, UMR8208 Mod&#xe9;lisation et simulation multi-&#xe9;chelle (MSME), France</p>
</fn>
<fn fn-type="edited-by">
<p>
<bold>Reviewed by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/2209696/overview">David Bassir</ext-link>, University of Technology of Belfort-Montb&#xe9;liard, France</p>
<p>
<ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/1229334/overview">Franziska Schmidt</ext-link>, Charit&#xe9; University Medicine Berlin, Germany</p>
</fn>
<corresp id="c001">&#x2a;Correspondence: Hong-Wu Wei, <email>nc.whw@163.com</email>, <email>ndfsyk0550@ncu.edu.cn</email>
</corresp>
</author-notes>
<pub-date pub-type="epub">
<day>16</day>
<month>07</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="collection">
<year>2025</year>
</pub-date>
<volume>12</volume>
<elocation-id>1625770</elocation-id>
<history>
<date date-type="received">
<day>09</day>
<month>05</month>
<year>2025</year>
</date>
<date date-type="accepted">
<day>01</day>
<month>07</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#xa9; 2025 Wu, Cao, Cao, Guo, Zhang, Yu, Wei and Wei.</copyright-statement>
<copyright-year>2025</copyright-year>
<copyright-holder>Wu, Cao, Cao, Guo, Zhang, Yu, Wei and Wei</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<p>The mechanical strength and stability of single taper-retained implant&#x2013;abutment connections in dental implantations have not been determined. We evaluated the mechanical performance of a single taper-retained implant system with two specifications (M1 and M2, with locking diameters being 2.0 and 2.5 mm, respectively). Static and dynamic loading, torsional strength, and lateral load tests were performed. M1 and M2 samples showed significantly different (<italic>P</italic> &#x3c; 0.05) static destructive powers (363.3 &#xb1; 22.32 and 583.6 &#xb1; 15.7 N, respectively). The dynamic maximum bending moment of both samples was 1276 N mm. M1 and M2 samples showed a yield torque of 114.8 &#xb1; 13.9 and 114.3 &#xb1; 6.9 N cm, a maximum yield torque of 130.0 &#xb1; 12.0 and 156.5 &#xb1; 6.6 N cm (<italic>P</italic> &#x3c; 0.05), an axial pull force of 179.8 &#xb1; 19.5 and 207.4 &#xb1; 13.7 N (<italic>P</italic> &#x3c; 0.05), and a lateral force of 140.2 &#xb1; 14.7 N and 238.15 &#xb1; 14.38 N (<italic>P</italic> &#x3c; 0.05). No damage or fracture was observed in abutments or implants after testing. The single taper-retained implant&#x2013;abutment connection showed high mechanical stability and durability, thus is a reliable replacement of the traditional screw-retained connection. Increasing the locking diameter of the abutment enhanced the mechanical strength of the connection.</p>
</abstract>
<kwd-group>
<kwd>single taper retaining</kwd>
<kwd>implant-abutment connection</kwd>
<kwd>solid abutment</kwd>
<kwd>mechanical strength</kwd>
<kwd>dental implantation</kwd>
<kwd>locking diameter</kwd>
</kwd-group>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Mechanics of Materials</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec id="s1">
<title>1 Introduction</title>
<p>Dental implantation is one of the primary treatment options for partially or fully edentulous patients and has a consistently high success rate (<xref ref-type="bibr" rid="B33">Pjetursson et al., 2014</xref>). Traditionally, a screw-retained connection is used to secure the abutment to the implant body. However, because this design requires the use of a central screw, it is often accompanied by mechanical complications such as damage and fracture that result from screw loosening due to excessive occlusal forces. The 5-year incidence rate of complications is 3.1%&#x2013;10.8% (<xref ref-type="bibr" rid="B33">Pjetursson et al., 2014</xref>). These complications can potentially lead to implant loss and even irreversible implant failure (<xref ref-type="bibr" rid="B25">Kim et al., 2021</xref>; <xref ref-type="bibr" rid="B5">Bischof et al., 2024</xref>).</p>
<p>To address the above issues, new implant&#x2013;abutment connection designs have been developed that take advantage of advancements in material technology to improve mechanical stability and reduce screw-related failures. The newest designs are primarily categorized into two types: external connection and internal connection. External connection is achieved by connecting a protrusion in the implant&#x2019;s upper plane with a concave area in the abutment&#x2019;s lower plane. Internal connection is achieved between a concave area in the implant and a protrusion in the abutment. Internal connections are associated with a lower incidence of bone resorption and mechanical complications and are more commonly used in clinical practice (<xref ref-type="bibr" rid="B8">Caricasulo et al., 2018</xref>; <xref ref-type="bibr" rid="B7">Camps-Font et al., 2023</xref>). Conical connections, also referred to as Morse taper connections (<xref ref-type="bibr" rid="B39">Schmitt et al., 2014</xref>), provide a tight fit between the implant and abutment, thereby minimizing bacterial penetration and improving long-term stability (<xref ref-type="bibr" rid="B38">Ricomini Filho et al., 2010</xref>; <xref ref-type="bibr" rid="B14">do Nascimento et al., 2012</xref>; <xref ref-type="bibr" rid="B28">Machado et al., 2013</xref>). Popular Morse taper systems include Ankylos (5.7&#xb0;), ITI (6&#xb0;&#x2013;8&#xb0;), and Astra Tech (11&#xb0;), all of which use screws for retention. In contrast, the Bicon system (1.5&#xb0;) uses a screwless Morse taper connection design that relies purely on frictional engagement for retention and thus eliminates the risk of complications that arise from screw loosening or fracture (<xref ref-type="bibr" rid="B42">Tang et al., 2017</xref>).</p>
<p>While Morse taper systems show acceptable implant survival rates (<xref ref-type="bibr" rid="B29">Mangano et al., 2011</xref>; <xref ref-type="bibr" rid="B46">Urdaneta et al., 2012</xref>) and predictability and demonstrate improved convenience in clinical practice, the performance of this system is still under debate. In a previous study on dental implant restoration complications, a screwless Morse taper system was associated with high rates of restoration dislodgement in the maxillary anterior area (<xref ref-type="bibr" rid="B47">Urdaneta et al., 2010</xref>). This may be because of two reasons. First, the low bone density and oblique occlusal loading in this area may increase the micromovement of the implant&#x2013;abutment interface. Second, restorations in the maxillary anterior area mainly experience forces that are non-axial, which can cause rotation or loosening of the abutment when the force becomes excessive. The same study also observed that Morse taper systems may have fractures in abutments with 2.0 mm locking diameters and dislodging in abutments with 3.0 mm locking diameters in the posterior areas (<xref ref-type="bibr" rid="B47">Urdaneta et al., 2010</xref>), likely because these areas are subject to greater chewing forces that cannot be sustained by narrow abutments. These findings suggest that the abutment diameter and loading patterns greatly affect the performance of Morse taper systems. However, clear understanding of the mechanical performance of screwless Morse taper connections remains limited because of the lack of data.</p>
<p>To fill this gap, we conducted <italic>in vitro</italic> tests to evaluate the mechanical strength of a screwless Morse taper implant&#x2013;abutment connection system, which adopts a narrow-diameter design that was less investigated before. Compared with previous works, we included more types of tests and also greatly increased the extent of mechanical challenge in the tests. Our findings provide useful insights and references for developing more advanced implant restoration strategies.</p>
</sec>
<sec sec-type="materials|methods" id="s2">
<title>2 Materials and methods</title>
<sec id="s2-1">
<title>2.1 Sample preparation</title>
<p>In this study, we developed a screwless Morse taper implant&#x2013;abutment system, with a connection design based on the commercial product from Bicon (Bicon Dental Implant, Boston, MA, United States). A total of 68 implants were machined using TA4G titanium alloy on a BO205-III precision lathe (Precision Tsugami Co., Kyoto, Japan) and included two types: M1 (3.3 &#xd7; 11 mm; batch No.: M120230320) and M2 (3.5 &#xd7; 14.5 mm; batch No.: M220230807) (<xref ref-type="fig" rid="F1">Figures 1</xref>, <xref ref-type="fig" rid="F2">2</xref>). The implants (M1, M2) were made by Jiangxi Zhiyale Medical Equipment Co., Ltd. (Nanchang, Jiangxi, China). Two types of solid abutments were produced with a locking diameter of 2.0 mm (Batch No.: ZJT20221009) and 2.5 mm (Batch No.: ZJT20230814) and with the internal Morse taper angle uniformly set as 1.5&#xb0; (<xref ref-type="fig" rid="F1">Figures 1</xref>, <xref ref-type="fig" rid="F2">2</xref>). A semispherical force-loading cap was added on the abutment. The designs and specifications of the implant and abutments are shown in <xref ref-type="fig" rid="F3">Figure 3</xref>. All implants and abutments were cleaned, polished, and inspected for precision (&#xb1;0.2 mm tolerance) before inclusion.</p>
<fig id="F1" position="float">
<label>FIGURE 1</label>
<caption>
<p>Photos of the abutment with 2.0-mm locking diameter <bold>(a)</bold>, the M1 implant <bold>(b)</bold>, the abutment with 2.5-mm locking diameter <bold>(c)</bold> and the M2 implant <bold>(d)</bold>.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g001.tif">
<alt-text content-type="machine-generated">Four components labeled a, b, c, and d are shown in a row. Items a and c are metallic cylindrical parts with narrow ends. Items b and d appear to be dark, ridged components, possibly screws or implants. Each has a 3mm scale for size reference.</alt-text>
</graphic>
</fig>
<fig id="F2" position="float">
<label>FIGURE 2</label>
<caption>
<p>Designs of the implant and abutment test samples. <bold>(a, d)</bold> Front view of the M1 <bold>(a)</bold> and M2 <bold>(d)</bold> implant. <bold>(b, e)</bold> Cross-sectional view of the M1 <bold>(a)</bold> and M2 <bold>(d)</bold> implant. <bold>(c, f)</bold> Front view of the abutment with 2.0-mm <bold>(c)</bold> and 2.5-mm <bold>(f)</bold> locking diameter.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g002.tif">
<alt-text content-type="machine-generated">Diagrams of dental implant components labeled a to f. a) Implant with 3.3 mm diameter and 11 mm height. b) Section with taper angle of 3 degrees. c) Abutment with 2.0 mm diameter. d) Implant with 3.5 mm diameter and 14.5 mm height. e) Section with taper angle of 3 degrees. f) Abutment with 2.5 mm diameter.</alt-text>
</graphic>
</fig>
<fig id="F3" position="float">
<label>FIGURE 3</label>
<caption>
<p>A photo <bold>(a)</bold> and the design <bold>(b)</bold> of the setup for static and dynamic loading tests. 1. Force loading module. 2. Nominal bone level. 3. Solid abutment. 4. Semispherical force-loading cap. 5. Implant main body. 6. Metal base.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g003.tif">
<alt-text content-type="machine-generated">A two-part image showing a precision measurement device and a related technical diagram. The left panel (a) depicts the close-up of a mechanical setup with a hemispherical tool aligned to make contact. The right panel (b) is a schematic diagram illustrating the angles and forces involved, labeled with indices such as 'F', 'A', 'B', and numerical identifiers for components.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s2-2">
<title>2.2 Equipment</title>
<p>The CMT6104 Microcomputer Controlled Electronic Universal Testing Machine (Shanghai Xinbiao Testing Instrument Manufacturing Co., Ltd., Shanghai, China) and BX53M Optical Microscope (Olympus, Tokyo, Japan) were used in this study.</p>
</sec>
<sec id="s2-3">
<title>2.3 Testing procedures</title>
<p>All tests were conducted under constant temperature (20&#xb0;C &#xb1; 10&#xb0;C) and humidity (40%&#x2013;60%) in a materials technology lab to simulate the physiological oral environment. The implant&#x2013;abutment system samples were embedded in ASTM F1839-compliant Grade 20 polyurethane foam blocks (simulating cancellous bones; Shenzhen Biosungreen Tech Co., Ltd., Shenzhen, Guangdong, China) following the manufacturer&#x2019;s instructions. Specifically, the block was pre-drilled, and the implant was screwed or tapped into the blocks; the abutment was mounted onto the implants by tapping. Then the connected implant and abutment were together removed from the block and mounted onto the universal testing machine.</p>
<p>For static and dynamic loading tests (n &#x3d; 16), the in-bone fixation part of the test sample was embedded and fixed, with the distance from the fixation level to the nominal bone level being 3.0 &#xb1; 0.5 mm and the distance from the center of the semispherical force-loading cap (C, <xref ref-type="fig" rid="F4">Figure 4</xref>) to the fixation level being l &#x3d; 11.0 &#xb1; 0.5 mm.</p>
<fig id="F4" position="float">
<label>FIGURE 4</label>
<caption>
<p>Setup for the torsional resistance test (left) and a representative torque vs. angular displacement curve (right).</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g004.tif">
<alt-text content-type="machine-generated">Close-up view of a metal testing apparatus on the left, holding a specimen for deformation tests. On the right, a graph shows a stress-strain curve with points Mmax, Mtors, A, B, S, and Oma, indicating material deformation behavior.</alt-text>
</graphic>
</fig>
<p>Static loading tests involved the application of a static force load by the Universal Testing Machine to the semispherical force-loading cap at an angle of 30&#xb0; to the implant&#x2019;s body axis and a rate of 5 mm/min, with an upper limit force of 232 N (<xref ref-type="fig" rid="F4">Figure 4</xref>). The force vs. displacement curve was monitored until the force reached maximum and began to decline, identifying the static destructive power. The sample was inspected to identify the affected part and the mode of damage was recorded, which could be material yielding, permanent deformation, or component loosening or breakage. The dynamic loading tests involved five million cycles of unidirectional force loading with an upper limit force of 232 N, a load ratio of 0.1, a sinusoidal loading waveform, and a frequency of 15 Hz. After the test was complete, the specified load force that was sustained by the test sample over five million loading cycles was recorded to calculate the corresponding bending moment, M.</p>
<p>For torsional resistance tests (n &#x3d; 12), both ends of the test sample were fixed using the collets on the micro-controlled torsion testing machine. Torque was applied at a rate of 5&#xb0;/min until the torque vs. angular displacement curve began to decline (<xref ref-type="fig" rid="F5">Figure 5</xref>). The yield torque and maximum yield torque were calculated using the curve.</p>
<fig id="F5" position="float">
<label>FIGURE 5</label>
<caption>
<p>Setup for the axial pull-out test.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g005.tif">
<alt-text content-type="machine-generated">Close-up of a metal stamping tool with numbers 359 visible on the upper part. The tool consists of a central rod and two pieces on the top and bottom, forming a press-like structure.</alt-text>
</graphic>
</fig>
<p>For axial pull-out tests (n &#x3d; 10), the test sample and its fixing collet were stabilized at the base of the force loading module while ensuring that the longitudinal axis of the test sample was aligned with the force load to be applied. The top part of the sample was subjected to axial tensile force at a pulling rate of 5 mm/min until detachment occurred (<xref ref-type="fig" rid="F6">Figure 6</xref>). The axial tensile force vs. displacement curve was recorded, and the axial pull force, i.e., the force needed to detach the implant from the abutment, was recorded.</p>
<fig id="F6" position="float">
<label>FIGURE 6</label>
<caption>
<p>Setup for the lateral load test.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g006.tif">
<alt-text content-type="machine-generated">A precise close-up of a CNC milling machine cutting a small cylindrical metal component. The machine's drill bit is aligned horizontally, working on a piece positioned beneath it, with focus on the detail and precision of the machining process.</alt-text>
</graphic>
</fig>
<p>For lateral load tests (n &#x3d; 10), the test sample was placed horizontally. A cantilever bending test was performed by applying a force perpendicular to the initial longitudinal axis of the sample at a rate of 5 mm/min until the force vs. displacement curve reached maximum and began to decline (<xref ref-type="fig" rid="F7">Figure 7</xref>). The length of the moment arm, the force vs. displacement curve, and the lateral force were recorded.</p>
<fig id="F7" position="float">
<label>FIGURE 7</label>
<caption>
<p>Setup for microscopic inspection.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g007.tif">
<alt-text content-type="machine-generated">Close-up of a microscope focusing on a small metallic object secured on a platform. The microscope lenses are labeled with magnification details in various colors: blue, red, and yellow.</alt-text>
</graphic>
</fig>
<p>For microscopic inspection (n &#x3d; 20), a high-magnification optical microscope was used to observe microstructural changes at the surface of implant samples after the axial pull-out or lateral load test (<xref ref-type="fig" rid="F8">Figure 8</xref>).</p>
<fig id="F8" position="float">
<label>FIGURE 8</label>
<caption>
<p>Statistical analysis of static test results for M1 and M2 samples. &#x2a;&#x2a;<italic>p</italic> &#x3c; 0.02; &#x2a;&#x2a;&#x2a;<italic>p</italic> &#x3c; 0.01; &#x2a;&#x2a;&#x2a;&#x2a;<italic>p</italic> &#x3c; 0.001, assessed by Student&#x2019;s t-test.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g008.tif">
<alt-text content-type="machine-generated">Bar graphs compare mechanical properties between M1 and M2. The left graph shows static destructive power (red), axial pull force (green), and lateral force (orange) in newtons. M2 performs better in destructive power and lateral force. The right graph depicts yield torque (blue) and maximum yield torque (purple) in newton-centimeters. M1 and M2 have similar yield torque, but M2 shows higher maximum yield torque. Statistical significance is indicated with asterisks.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s2-4">
<title>2.4 Statistical analysis</title>
<p>G&#x2a;Power v 3.1.9.7 was used to calculate statistical power for the tests and determine the appropriate sample size for each test. Data analysis was conducted using SPSS 26.0 (IBM, Armonk, NY, United States). Data with a normal distribution, as judged by the Kolmogorov&#x2013;Smirnov test, are presented as mean &#xb1; standard deviation, with independent sample t-test used for data comparison. A <italic>p</italic>-value less than 0.05 indicated statistical significance.</p>
</sec>
</sec>
<sec sec-type="results" id="s3">
<title>3 Results</title>
<sec id="s3-1">
<title>3.1 Static and dynamic loading tests</title>
<p>A significantly higher static destructive power was identified in the M2 samples than in the M1 samples (583.6 &#xb1; 15.7 vs. 363.3 &#xb1; 22.3 N), indicating better mechanical performance of the M2 samples (<xref ref-type="table" rid="T1">Table 1</xref>; <xref ref-type="fig" rid="F9">Figure 9</xref>). Detailed static loading test curves are presented in <xref ref-type="fig" rid="F10">Figure 10</xref>.</p>
<table-wrap id="T1" position="float">
<label>TABLE 1</label>
<caption>
<p>Results of the static tests of M1 and M2 samples.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Test parameter</th>
<th align="left">M1</th>
<th align="left">
<italic>95% CI</italic>
</th>
<th align="left">M2</th>
<th align="left">
<italic>P</italic>
</th>
<th align="left">
<italic>95% CI</italic>
</th>
<th align="left">
<italic>Effect size</italic>
</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">Static destructive power (N)</td>
<td align="left">363.3 &#xb1; 22.3</td>
<td align="left">361.3&#x2013;365.3</td>
<td align="left">583.6 &#xb1; 15.7</td>
<td align="left">0.000</td>
<td align="left">569.6&#x2013;597.6</td>
<td align="left">0.98</td>
</tr>
<tr>
<td align="left">Yield torque (N&#xb7;cm)</td>
<td align="left">114.8 &#xb1; 13.9</td>
<td align="left">103.8&#x2013;125.8</td>
<td align="left">114.3 &#xb1; 6.9</td>
<td align="left">0.947</td>
<td align="left">108.7&#x2013;119.9</td>
<td align="left">0.02</td>
</tr>
<tr>
<td align="left">Maximum yield torque (N&#xb7;cm)</td>
<td align="left">130.0 &#xb1; 12.0</td>
<td align="left">119.0&#x2013;141.0</td>
<td align="left">156.5 &#xb1; 6.6</td>
<td align="left">0.001</td>
<td align="left">151.2&#x2013;161.8</td>
<td align="left">0.78</td>
</tr>
<tr>
<td align="left">Axial pull force (N)</td>
<td align="left">179.8 &#xb1; 19.5</td>
<td align="left">167.8&#x2013;191.8</td>
<td align="left">207.4 &#xb1; 13.7</td>
<td align="left">0.002</td>
<td align="left">198.9&#x2013;215.9</td>
<td align="left">0.63</td>
</tr>
<tr>
<td align="left">Lateral force (N)</td>
<td align="left">140.2 &#xb1; 14.7</td>
<td align="left">127.2&#x2013;153.2</td>
<td align="left">238.1 &#xb1; 14.3</td>
<td align="left">0.000</td>
<td align="left">225.2&#x2013;251.2</td>
<td align="left">0.96</td>
</tr>
</tbody>
</table>
</table-wrap>
<fig id="F9" position="float">
<label>FIGURE 9</label>
<caption>
<p>Static loading test curves of M1 <bold>(a)</bold> and M2 <bold>(b)</bold> samples.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g009.tif">
<alt-text content-type="machine-generated">Two graphs labeled a and b comparing force over distance. Graph a shows curves peaking between 273 and 429 N, then leveling. Graph b shows curves peaking around 549 to 671 N, then rapidly declining. Both graphs feature multiple lines denoting different data sets over the same x-axis ranging from 0 to 3.6 distance units.</alt-text>
</graphic>
</fig>
<fig id="F10" position="float">
<label>FIGURE 10</label>
<caption>
<p>Torsional resistance test curves of the M1 <bold>(a)</bold> and M2 <bold>(b)</bold> samples.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g010.tif">
<alt-text content-type="machine-generated">Two line graphs labeled &#x22;a&#x22; and &#x22;b&#x22; display data trends. Graph &#x22;a&#x22; shows multiple colored lines on a grid, indicating steady trends from zero to two hundred twenty on the horizontal axis. Graph &#x22;b&#x22; also has multiple colored lines, showing rising trends from zero to forty-four on the horizontal axis, with peaks around thirty-five, then declining. The vertical axis for both graphs ranges from zero to two.</alt-text>
</graphic>
</fig>
<p>All implants and abutments survived the dynamic loading test without failure, yielding an overall survival rate of 100%. The dynamic maximum bending moment was 1276 N mm for both M1 and M2 samples.</p>
</sec>
<sec id="s3-2">
<title>3.2 Torsional resistance test</title>
<p>M1 samples rendered a yield torque of 114.8 &#xb1; 13.9 N cm and a maximum yield torque of 130.0 &#xb1; 12.0. While M2 samples rendered a comparable yield torque of 114.3 &#xb1; 6.9 N cm, the maximum yield torque reached a significantly higher value of 156.5 &#xb1; 6.6 N cm (<italic>P</italic> &#x3c; 0.05, <xref ref-type="table" rid="T1">Table 1</xref>; <xref ref-type="fig" rid="F9">Figure 9</xref>).</p>
<p>The torsional resistance test curves are presented in <xref ref-type="fig" rid="F11">Figure 11</xref>.</p>
<fig id="F11" position="float">
<label>FIGURE 11</label>
<caption>
<p>Lateral load test curves of the M1 <bold>(a)</bold> and M2 <bold>(b)</bold> samples.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g011.tif">
<alt-text content-type="machine-generated">Two line graphs labeled &#x22;a&#x22; and &#x22;b&#x22; depict different datasets with overlapping lines. Graph &#x22;a&#x22; ranges from zero to one hundred ninety-eight on the y-axis, and zero to thirteen on the x-axis. Graph &#x22;b&#x22; ranges from zero to three hundred eight on the y-axis, and zero to sixteen on the x-axis. Multiple colored lines indicate different data series, showing upward trends with some fluctuations.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s3-3">
<title>3.3 Axial pull-out test</title>
<p>M1 samples rendered an axial pull force of 179.8 &#xb1; 19.5 N M2 samples rendered a significantly higher axial pull force of 207.4 &#xb1; 13.7 N (<italic>P</italic> &#x3c; 0.05, <xref ref-type="table" rid="T1">Table 1</xref>).</p>
</sec>
<sec id="s3-4">
<title>3.4 Lateral load test</title>
<p>M1 samples rendered a lateral force of 140.2 &#xb1; 14.7 N M2 samples rendered a significantly higher lateral force of 238.1 &#xb1; 14.3 N (<italic>P</italic> &#x3c; 0.05, <xref ref-type="table" rid="T1">Table 1</xref>).</p>
<p>The lateral load test curves are presented in <xref ref-type="fig" rid="F12">Figure 12</xref>.</p>
<fig id="F12" position="float">
<label>FIGURE 12</label>
<caption>
<p>Post-test microscopic inspection of the implants and abutments. <bold>(a&#x2013;f)</bold> Appearance of the implant and abutment for M1 <bold>(a&#x2013;c)</bold> and M2 <bold>(d&#x2013;f)</bold> samples before and after axial pull-out test. <bold>(g&#x2013;l)</bold> The appearance of the implant and abutment for M1 <bold>(g&#x2013;i)</bold> and M2 <bold>(j&#x2013;l)</bold> samples before and after lateral load test.</p>
</caption>
<graphic xlink:href="fmats-12-1625770-g012.tif">
<alt-text content-type="machine-generated">Microscopic images arranged in a grid with labeled sections a to l, showing the surface texture of a material with a scale of five hundred micrometers for reference. Each section displays varying structural patterns and light reflections, indicating differences in the material's surface finish or treatment.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s3-5">
<title>3.5 Post-test microscopic inspection</title>
<p>No crack or surface damage was detected on any of the implants or abutments after completion of the axial pull-out and lateral load tests (<xref ref-type="fig" rid="F12">Figure 12</xref>).</p>
</sec>
</sec>
<sec sec-type="discussion" id="s4">
<title>4 Discussion</title>
<p>By simulating the chewing motion, this study evaluated the mechanical performance of a single taper-retained dental implant&#x2013;abutment system and inspected how the system is impacted by the size of the implant&#x2013;abutment connection interface. The results demonstrated that the single taper-retained connection demonstrated excellent stability while producing minimal micromotion and wear under various high-stress conditions; this system should therefore help prolong the service life of the implants. These findings not only enhance the understanding of single taper-retained implant&#x2013;abutment systems, but also provide useful references for clinicians and may help improve the success rate of dental implantation and patient satisfaction.</p>
<p>The single taper-retained connection system tested in this study involved a screwless design with an internal connection taper angle of 1.5&#xb0;. Retention of this system relies entirely on the friction generated by the tight contact between the implant and the abutment. During implantation, the implant is tapped onto the abutment with external force, which produces a wedging effect at the implant&#x2013;abutment interface (IAC) to achieve a tight connection&#x2014;sometimes referred to as &#x201c;cold welding.&#x201d; This novel connection mechanism has several advantages over traditional mechanisms. First, it constrains the microgap at the coronal aspect of the IAC, which ensures superior sealing and stability, significantly reducing bacterial penetration and lowering the risk of infection (<xref ref-type="bibr" rid="B4">Baixe et al., 2010</xref>; <xref ref-type="bibr" rid="B9">Chu et al., 2012</xref>; <xref ref-type="bibr" rid="B27">Liu and Wang, 2017</xref>; <xref ref-type="bibr" rid="B18">Gao et al., 2021</xref>). Second, the abutment has a solid cylindrical structure, which has high mechanical strength, also ensuring high stability at the peri-implant bone levels and a long service life of the implants (<xref ref-type="bibr" rid="B36">Ribeiro et al., 2024</xref>). Finally, owing to the high-precision manufacturing of the implant&#x2013;abutment interface, consistency can be ensured across implantations.</p>
<p>Static and dynamic loading tests are commonly used to evaluate the mechanical strength of implant&#x2013;abutment connections. For instance, Dittmer et al. studied the fracture strength of Ankylos Morse taper implants by applying a 30&#xb0; oblique load; the failure load was 368 N for the screw-retained connection and 430 N for the Morse taper connection (<xref ref-type="bibr" rid="B13">Dittmer et al., 2012</xref>). Imam et al. investigated the static load strength of narrow-diameter implant systems; the average static destructive power was 367.20 &#xb1; 98.05 and 568.80 &#xb1; 85.24 N for implants with a 3.0 mm or 3.5 mm connection diameter, respectively (<xref ref-type="bibr" rid="B24">Imam et al., 2014</xref>). Marchetti et al. conducted fatigue tests on implant systems with an internal hex connection of 3.8-mm diameter; the results showed that the static destructive power was 499.6 &#xb1; 50.1 N (<xref ref-type="bibr" rid="B30">Marchetti et al., 2014</xref>). Duan et al. conducted fatigue tests on implant systems with a 3.3 mm connection diameter; under a cyclic load of 185 N, the probability of failure after 1 &#xd7; 10<sup>6</sup> dynamic loading cycles was 5% (<xref ref-type="bibr" rid="B15">Duan et al., 2018</xref>). The study suggested that, compared with narrow-diameter implants, implants with the same connection diameter but using the Morse taper connection mechanism achieved higher static and fatigue load capacity. Notably, in our study, the static destructive powers of the M1 and M2 implants both exceeded those of the previously reported Morse taper and screw-retained connections. Furthermore, Dittmer et al. showed that the internal connection diameter of the implant has a significant influence on its overall mechanical strength (<xref ref-type="bibr" rid="B13">Dittmer et al., 2012</xref>), which corroborates our findings in comparing M1 and M2.</p>
<p>Humans can generate great bite forces (<xref ref-type="bibr" rid="B48">Wang, 2012</xref>; <xref ref-type="bibr" rid="B50">Zhou et al., 2022</xref>). Ferrario et al. reported that in healthy young adults, the occlusal force on a single tooth is between 98.33 and 306.07 N (<xref ref-type="bibr" rid="B17">Ferrario et al., 2004</xref>). Shoji et al. found that occlusal forces in healthy adults ranged from 86.40 to 1758.60 N, with an average of 798.33 &#xb1; 492.16 N (<xref ref-type="bibr" rid="B41">Shoji et al., 2022</xref>). After an implant&#x2013;abutment system experiences more and more load cycles with such large forces, it is expected to have gradually declined mechanical performance (<xref ref-type="bibr" rid="B37">Ricciardi Coppede et al., 2009</xref>). Nonetheless, in this study, both M1 and M2 implant&#x2013;abutment connections withstood 5 &#xd7; 10<sup>6</sup> cycles of dynamic loading at 23.2&#x2013;232 N, demonstrating excellent mechanical durability.</p>
<p>By performing <italic>in vitro</italic> fatigue tests, Ugurel et al. found that the mechanical strength of screwless Morse taper implants with a 3&#xb0; taper angle was lower than that of screw-retained implants. None of the tested screwless Morse taper implants survived 1.2 &#xd7; 10<sup>6</sup> cycles of dynamic loading of 120 N applied at a 30&#xb0; angle; all samples failed within the first 1 &#xd7; 10<sup>5</sup> cycles as a result of fracture (<xref ref-type="bibr" rid="B45">Ugurel et al., 2015</xref>). In contrast, in the study by Bagegni et al., all tested screwless Morse taper implants survived 1 &#xd7; 10<sup>6</sup> cycles of dynamic loading of 100 N (<xref ref-type="bibr" rid="B2">Bagegni et al., 2022</xref>). The discrepancy between these two studies may be because the implants tested in the first study had a thinner internal wall. Here, our tested samples also successfully survived 5 &#xd7; 10<sup>6</sup> cycles of dynamic loading applied at a 30&#xb0; angle, supporting the conclusion that single taper-retained connections can achieve high mechanical strength. In contrast with the results from <xref ref-type="bibr" rid="B45">Ugurel et al. (2015)</xref>, the screwless implant samples in our study showed no surface cracks or abutment fractures after testing, consistent with <xref ref-type="bibr" rid="B2">Bagegni et al. (2022)</xref>.</p>
<p>Screw loosening or fracture is the most common mechanical complication in dental implant restorations (<xref ref-type="bibr" rid="B40">Schwartz-Arad et al., 1999</xref>; <xref ref-type="bibr" rid="B44">Theoharidou et al., 2008</xref>). The likelihood of screw loosening and fracture is influenced by the design, material, and diameter of the screw, the applied torque, and the geometry of the implant&#x2013;abutment connection (<xref ref-type="bibr" rid="B21">Gupta et al., 2015</xref>), but was reported to be considerably high across multiple studies (<xref ref-type="bibr" rid="B20">Goodacre et al., 1999</xref>; <xref ref-type="bibr" rid="B32">Pintinha et al., 2013</xref>; <xref ref-type="bibr" rid="B22">Hsu et al., 2018</xref>; <xref ref-type="bibr" rid="B34">Pjetursson et al., 2018</xref>). In our study, none of the tested implants showed abutment fracture after five million mastication-simulating load cycles, demonstrating their mechanical reliability and superiority. The main reason for screw loosening after implantation is that the dynamic load cycles during mastication may cause the vertical subsidence of the abutment, which can induce slippage between implant threads and screw threads, resulting in torque loss and screw loosening (<xref ref-type="bibr" rid="B26">Kim et al., 2014</xref>; <xref ref-type="bibr" rid="B3">Bagegni et al., 2021</xref>). We previously found that during simulated mastication, the total vertical displacement of screwless Morse taper abutments ranged from 0.20 to 0.47 mm (average: 0.32 mm), and the finger-pressed displacement ranged from 0.06 to 0.15 mm (average: 0.10 mm); the degree of subsidence was affected by the loading force, locking depth, locking diameter, and thickness of the implant&#x2019;s external wall (<xref ref-type="bibr" rid="B35">Ren et al., 2024</xref>). Further <italic>in vitro</italic> studies are needed to determine the optimal design for related key parameters such as locking diameter and depth, so as to prevent or minimize abutment subsidence in patients.</p>
<p>For screw-retained implant&#x2013;abutment connections, in addition to repeated screw tightening (<xref ref-type="bibr" rid="B12">D&#x2019;Addazio et al., 2021</xref>), the geometry of the connection also affects the pull-out resistance. For instance, smaller angles were found to offer greater resistance (<xref ref-type="bibr" rid="B6">Caballero et al., 2024</xref>). A similar result was observed for screwless connections as well (<xref ref-type="bibr" rid="B31">Nogueira Barbosa Marchon et al., 2024</xref>), which is likely because of two reasons. First, a smaller taper angle leads to a greater contact area, which increases the friction at the implant&#x2013;abutment interface and thus strengthens the cold welding effect (<xref ref-type="bibr" rid="B6">Caballero et al., 2024</xref>; <xref ref-type="bibr" rid="B31">Nogueira Barbosa Marchon et al., 2024</xref>). Second, Hsu et al. showed that smaller taper angles in implant&#x2013;abutment connections may result in greater axial displacement, thereby increasing friction and thus the pull-out resistance (<xref ref-type="bibr" rid="B22">Hsu et al., 2018</xref>). Like in our work, Hsu et al. also knocked the upper component into the implant to achieve the connection, which led to pull-out resistance and mechanical stability comparable to the screw-retained connections (<xref ref-type="bibr" rid="B22">Hsu et al., 2018</xref>). The implants in our study use an internal taper of 1.5&#xb0; with screwless solid abutments that maximize the implant&#x2013;abutment contact area, ensuring adequate pull-out resistance. The satisfactory pull-out test results of these implants are strongly encouraging and indicate that alternative implant&#x2013;abutment connection designs should be considered to overcome the issue of screw loosening in traditional designs. In addition, our data suggests that increasing the locking diameter and thus the frictional contact surface can also increase the axial pull force.</p>
<p>A higher torque in screw-based systems leads to greater preload, which reduces the risk of screw loosening and restoration dislodgement (<xref ref-type="bibr" rid="B10">Cibirka et al., 2001</xref>; <xref ref-type="bibr" rid="B11">Coelho et al., 2007</xref>). However, excessive torque beyond a threshold can create significant friction between the implant and screw threads, which can potentially lead to wrench fracture. Hyun et al. found that adding a positioning hex design to a Morse taper abutment ensures a snug fit with the implant&#x2019;s corresponding hex slot, and thus can effectively increase torsional resistance (<xref ref-type="bibr" rid="B23">Hyun et al., 2020</xref>). However, adding a hex design to a Morse taper abutment also reduces the conical surface area of the abutment, which may compromise the biomechanical performance of the Morse taper connection. Yao et al. demonstrated that in the Cowell implant system with a 7&#xb0; taper, the single taper-retained connection offered no torsional resistance. The authors found that incorporating an internal octagonal structure provided torsional resistance, but it also lowered the bending strength of the abutment (<xref ref-type="bibr" rid="B49">Yao et al., 2015</xref>). Farr&#xe9;-Berga et al. showed that with a 20&#xb0; angle in the screw channel, connections in the Ball Head System and the Hexagonal Screwdriver System had a mean yield torque of 67.0 &#xb1; 12.0 and 45.0 &#xb1; 2.0 N cm, respectively (<xref ref-type="bibr" rid="B16">Farre-Berga et al., 2020</xref>). In our torsional resistance tests, M1 and M2 samples showed a maximum yield torque of 130.0 &#xb1; 12.0 and 156.5 &#xb1; 6.6 N cm, respectively, which were substantially higher than those reported by <xref ref-type="bibr" rid="B16">Farre-Berga et al. (2020)</xref>, even though our abutments use no anti-torsion features. This result suggests that increasing the contact area and friction between the abutment and the implant effectively strengthens the cold welding effect, ensuring reliable torsional stability.</p>
<p>The performance of the implant&#x2013;abutment connection plays a crucial role in the successful implantation of implant-supported fixed restorations. The abutment is connected to the implant via external or internal connection mechanisms, with the internal connection being biomechanically advantageous. Peri-implantitis and marginal bone loss are two common complications of implant-supported restorations that are induced by the gap space at the implant&#x2013;abutment interface, with the former often preceding and facilitating the latter. Under repetitive lateral occlusal loading, traditional screw-retained systems, even those with an internal hex design, are prone to screw loosening, which increases the gap space at the implant&#x2013;abutment interface, leading to bacterial infiltration and subsequent bone resorption, especially in jaw areas of low bone density (<xref ref-type="bibr" rid="B19">Gehrke et al., 2023</xref>; <xref ref-type="bibr" rid="B1">Angermair et al., 2024</xref>). In contrast, because of its self-locking design, the Morse taper connection offers superior lateral stability, which does not form microgaps under lateral forces (<xref ref-type="bibr" rid="B1">Angermair et al., 2024</xref>).</p>
<p>Terrats et al. studied the lateral mechanical strength of single-unit and multi-unit restorations with a narrow diameter implant; the lateral failure force was 118&#x2013;230 and 488&#x2013;759 N, respectively, demonstrating clearly greater lateral load resistance in the multi-unit restorations (<xref ref-type="bibr" rid="B43">Terrats et al., 2024</xref>). Our study showed that M1 and M2 implants had a lateral resistance of 140.2 &#xb1; 14.7 and 238.1 &#xb1; 14.3 N, respectively, which are relatively low but within clinically acceptable limits. Furthermore, comparison of the results of M1 and M2 indicates that increasing the abutment diameter enhances the implant&#x2019;s fracture resistance and lateral load-bearing capacity. In anterior regions, implant restorations may endure non-axial forces 4&#x2013;5 times greater than normal occlusal forces. Such lateral loading can induce micromotion and microgaps, potentially causing marginal bone resorption. Through continued design optimization (e.g., reducing the taper angle or increasing the contact area), researchers should be able to improve the lateral stability of the implant&#x2013;abutment connection.</p>
<p>Overall, under standardized conditions, all samples tested in this work withstood fatigue loading, indicating that the single taper-retained implant&#x2013;abutment connection represents a reliable option for dental restorations. Nonetheless, due to certain limitations of our work such as small sample sizes and lack of high-resolution microscopic imaging (e.g., scanning electron microscopy), as well as the fact that <italic>in vitro</italic> conditions cannot fully recapitulate the oral environment, further standardized tests are needed to more comprehensively analyze the mechanical behavior of single taper-retained implant systems.</p>
</sec>
<sec sec-type="conclusion" id="s5">
<title>5 Conclusion</title>
<p>This <italic>in vitro</italic> study demonstrated that a single taper-retained implant&#x2013;abutment connection offers strong mechanical performance under both static and long-term dynamic loading conditions. Increasing the locking diameter of the abutment improves the mechanical performance of the connection.</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="s6">
<title>Data availability statement</title>
<p>The raw data supporting the conclusion of this article will be made available by the authors, without undue reservation.</p>
</sec>
<sec sec-type="author-contributions" id="s7">
<title>Author contributions</title>
<p>HW: Writing &#x2013; original draft, Formal Analysis, Data curation, Conceptualization, Investigation. NC: Data curation, Investigation, Writing &#x2013; review and editing. L-WC: Data curation, Investigation, Writing &#x2013; review and editing. S-GG: Conceptualization, Writing &#x2013; review and editing, Methodology. XZ: Writing &#x2013; review and editing, Data curation, Resources. FY: Resources, Data curation, Writing &#x2013; review and editing. S-BW: Writing &#x2013; review and editing, Resources, Data curation. H-WW: Methodology, Project administration, Writing &#x2013; review and editing.</p>
</sec>
<sec sec-type="funding-information" id="s8">
<title>Funding</title>
<p>The author(s) declare that no financial support was received for the research and/or publication of this article.</p>
</sec>
<sec sec-type="COI-statement" id="s9">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="ai-statement" id="s10">
<title>Generative AI statement</title>
<p>The author(s) declare that no Generative AI was used in the creation of this manuscript.</p>
</sec>
<sec sec-type="disclaimer" id="s11">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
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