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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Mar. Sci.</journal-id>
<journal-title>Frontiers in Marine Science</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Mar. Sci.</abbrev-journal-title>
<issn pub-type="epub">2296-7745</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fmars.2025.1613955</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Marine Science</subject>
<subj-group>
<subject>Review</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Pressure-holding transfer and testing techniques for natural gas hydrate reservoir core: a brief review</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Gao</surname>
<given-names>Qiaoling</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/2023097/overview"/>
<role content-type="https://credit.niso.org/contributor-roles/funding-acquisition/"/>
<role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Chen</surname>
<given-names>Jiawang</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/1658493/overview"/>
<role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/>
<role content-type="https://credit.niso.org/contributor-roles/funding-acquisition/"/>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Nan</surname>
<given-names>Liwen</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="author-notes" rid="fn001">
<sup>*</sup>
</xref>
<role content-type="https://credit.niso.org/contributor-roles/investigation/"/>
<role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Shen</surname>
<given-names>Jiayi</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<role content-type="https://credit.niso.org/contributor-roles/funding-acquisition/"/>
<role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/>
</contrib>
</contrib-group>
<aff id="aff1">
<sup>1</sup>
<institution>Ocean Robotics Center, Donghai Laboratory</institution>, <addr-line>Zhoushan</addr-line>,&#xa0;<country>China</country>
</aff>
<aff id="aff2">
<sup>2</sup>
<institution>Institute of Ocean Engineering and Technology, Ocean College, Zhejiang University</institution>, <addr-line>Zhoushan</addr-line>,&#xa0;<country>China</country>
</aff>
<author-notes>
<fn fn-type="edited-by">
<p>Edited by: David Alberto Salas de Le&#xf3;n, National Autonomous University of Mexico, Mexico</p>
</fn>
<fn fn-type="edited-by">
<p>Reviewed by: <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/1981248/overview">Zhaoran Wu</ext-link>, Yanshan University, China</p>
<p>
<ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/3131885/overview">Yufei Chen</ext-link>, Southwest Petroleum University, China</p>
</fn>
<fn fn-type="corresp" id="fn001">
<p>*Correspondence: Liwen Nan, <email xlink:href="mailto:nanliwen@163.com">nanliwen@163.com</email>
</p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>05</day>
<month>09</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="collection">
<year>2025</year>
</pub-date>
<volume>12</volume>
<elocation-id>1613955</elocation-id>
<history>
<date date-type="received">
<day>18</day>
<month>04</month>
<year>2025</year>
</date>
<date date-type="accepted">
<day>21</day>
<month>08</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#xa9; 2025 Gao, Chen, Nan and Shen.</copyright-statement>
<copyright-year>2025</copyright-year>
<copyright-holder>Gao, Chen, Nan and Shen</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<p>Uncovering the dynamic evolution mechanism of natural gas hydrate reservoir properties is crucial for realizing efficient resource exploration and safe trial mining. Obtaining high-quality hydrate reservoir cores and conducting comprehensive testing and analysis are important prerequisites for obtaining real information about hydrate reservoir. Pressure-holding transfer and testing of natural gas hydrate reservoir cores is a powerful means of accumulating <italic>in situ</italic> physical analysis data. This report summarizes the current state of development of pressure-holding transfer and testing techniques for natural gas hydrate reservoir cores from both research and application aspects. The advantages and disadvantages of existing gas hydrate reservoir core pressure-holding transfer and testing systems are comprehensively reviewed. The basic methods of pressure core processing, including buckling decomposition, permeability measurement, triaxial testing and integrated measurement, are analyzed, as well as the advantages and disadvantages of these methods. These reviews not only help researchers to better understand the current status of pressure-holding transfer and testing techniques for natural gas hydrate reservoir cores, but also have important reference significance for the in-depth development of future hydrate reservoir core testing technology.</p>
</abstract>
<kwd-group>
<kwd>natural gas hydrate</kwd>
<kwd>pressure core</kwd>
<kwd>transfer and analysis</kwd>
<kwd>triaxial testing</kwd>
<kwd>automated measurement</kwd>
</kwd-group>
<counts>
<fig-count count="18"/>
<table-count count="4"/>
<equation-count count="0"/>
<ref-count count="62"/>
<page-count count="19"/>
<word-count count="8176"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-in-acceptance</meta-name>
<meta-value>Ocean Observation</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec id="s1" sec-type="intro">
<label>1</label>
<title>Introduction</title>
<p>Natural gas hydrate is a natural gas resource with millions of years of stability discovered in the 1960s, which has the characteristics of high energy density, cleanliness, abundant resources (<xref ref-type="bibr" rid="B26">Lu, 2015</xref>; <xref ref-type="bibr" rid="B29">Makogon et&#xa0;al., 2007</xref>; <xref ref-type="bibr" rid="B18">Johnson and Max, 2006</xref>; <xref ref-type="bibr" rid="B56">Yu et&#xa0;al., 2021</xref>). It is a strategic energy source with the potential for sustainable development, and is receiving the attention of governments and scientists in various countries (<xref ref-type="bibr" rid="B5">Boswell, 2007</xref>; <xref ref-type="bibr" rid="B37">Sain, 2017</xref>; <xref ref-type="bibr" rid="B21">Lee and Holder, 2001</xref>; <xref ref-type="bibr" rid="B4">Borah et&#xa0;al., 2024</xref>). At a time when conventional energy sources are becoming increasingly scarce, countries around the world, such as the United States, Canada, Japan, India, China and South Korea, are competing for natural gas hydrate resource exploration in their respective economic waters (<xref ref-type="bibr" rid="B20">Kvenvolden and Lorenson, 2001</xref>; <xref ref-type="bibr" rid="B31">Pang, 2023</xref>; <xref ref-type="bibr" rid="B48">Veluswamy and Upadhye, 2022</xref>). The latest data on the projected resource potential of natural gas hydrates show that global carbon resources in hydrates range from 500 to 3,000 Gt, which is twice as much as the total amount of carbon in the form of fossil fuels on Earth (<xref ref-type="bibr" rid="B11">Diaconescu and Knapp, 2000</xref>; <xref ref-type="bibr" rid="B10">Diaconescu et&#xa0;al., 2001</xref>; <xref ref-type="bibr" rid="B32">Pang et&#xa0;al., 2021</xref>). Between 2020 and 2030, gas hydrate is expected to enter small-scale commercial production and eventually become a major global natural gas supply source (<xref ref-type="bibr" rid="B21">Lee and Holder, 2001</xref>; <xref ref-type="bibr" rid="B12">Fazioli, 2024</xref>; <xref ref-type="bibr" rid="B30">Nandi et&#xa0;al., 2022</xref>).</p>
<p>Natural gas hydrate is both a potential unconventional energy source and a possible geological disaster trigger with significant environmental impacts. The development of natural gas hydrate resources, disaster prevention and control, and environmental assessment all need to vigorously carry out basic research on the physicochemical and mechanical properties of natural gas hydrate reservoirs (<xref ref-type="bibr" rid="B24">Liu et&#xa0;al., 2017</xref>; <xref ref-type="bibr" rid="B22">Li et&#xa0;al., 2018</xref>; <xref ref-type="bibr" rid="B19">Krey et&#xa0;al., 2009</xref>; <xref ref-type="bibr" rid="B8">Chen et&#xa0;al., 2024</xref>). At present, due to both technical and cost constraints, these basic researches are still based on artificially prepared natural gas hydrate core samples, based on which the data, conclusions, and understandings obtained are quite different from the field reservoir conditions (<xref ref-type="bibr" rid="B49">Wei et&#xa0;al., 2022</xref>; <xref ref-type="bibr" rid="B28">Ma et&#xa0;al., 2023</xref>). Although it is recognized that <italic>in-situ</italic> studies of gas hydrate formations should be prioritized when conditions permit, some of the fine analysis can only be done in the laboratory (<xref ref-type="bibr" rid="B51">Yamamoto, 2015</xref>; <xref ref-type="bibr" rid="B17">Inada and Yamamoto, 2015</xref>). Pressure cores recover gas hydrate preserved within the sedimentary matrix, without the disruption caused by gas hydrate dissociation or dissolved gas exsolution. These samples offer the most pristine representations of gas hydrate formations, making them ideal for lab analysis&#x2014;including geophysical studies, geomechanical testing, and hydrate quantification (<xref ref-type="bibr" rid="B59">Yun et&#xa0;al., 2006</xref>; <xref ref-type="bibr" rid="B25">Liu et&#xa0;al., 2023</xref>; <xref ref-type="bibr" rid="B50">Xue et&#xa0;al., 2024</xref>).</p>
<p>Pressure retaining coring can maintain the <italic>in-situ</italic> temperature and pressure conditions of the core to the maximum extent during drilling, avoid decomposition of natural gas hydrate, and ensure that the composition of the core phases, pore structure and physical properties of the core do not differ much from its <italic>in-situ</italic> state. At present, the pressure retaining coring technology has been relatively mature, and many scholars (<xref ref-type="bibr" rid="B2">Abid et&#xa0;al., 2015</xref>; <xref ref-type="bibr" rid="B15">He et&#xa0;al., 2020</xref>; <xref ref-type="bibr" rid="B1">Abegg et&#xa0;al., 2008</xref>; <xref ref-type="bibr" rid="B27">Lu et al., 2025</xref>) have summarized and compared the key technologies and structural characteristics of the pressure retaining coring system for natural gas hydrate reservoirs, but there are fewer summaries of the current status of the pressure-holding transfer and testing technologies for the cores of natural gas hydrate reservoirs.</p>
<p>In this review, we firstly summarize the research progress of the current stage of pressure-holding transfer and testing technology for natural gas hydrate reservoir cores, and introduce in detail the typical core pressure-holding transfer and testing systems established for natural gas hydrates. Secondly, we analyze the key technologies of pressure core processing, including buckling decomposition, permeability measurement, and triaxial testing. Finally, we summarize the difficulties of pressure-holding testing and analysis techniques for natural gas hydrate reservoir cores, and prospect their future development trends. Researchers can discover the problems of existing technologies through this review, and provide theoretical basis and practical guidance for subsequent research.</p>
</sec>
<sec id="s2">
<label>2</label>
<title>Development and application of pressure core transfer and testing system</title>
<p>The pressure core transfer and testing system is the core platform that connects the pressure corer with scientific research, supporting the transfer of cores from the high-pressure coring chamber to the analysis chamber for non-destructive testing (geophysical parameter measurements and X-ray imaging), sample splitting, and subsequent encapsulation into a pressure vessel for easy transportation or further experimentation (<xref ref-type="bibr" rid="B33">Parkes et al., 2009</xref>). This chapter introduces the current research progress of pressure core transfer and testing technology, and analyzes the functional advantages and limitations of several major pressure core transfer and testing systems.</p>
<sec id="s2_1">
<label>2.1</label>
<title>PCATS (pressure core analysis and transfer system)</title>
<p>PCATS was developed by Geotek and put into use in 2005 in the U.S. Gulf of Mexico gas hydrate drilling project (GOMJIPLeg1), and has been involved in all the gas hydrate drilling projects around the world, and its structure and functions have been continuously improved and optimized in many applications (<xref ref-type="bibr" rid="B41">Schultheiss et&#xa0;al., 2010</xref>). The PCATS system is installed in three standard size 20-foot ISO containers as shown in <xref ref-type="fig" rid="f1">
<bold>Figure&#xa0;1</bold>
</xref> (<xref ref-type="bibr" rid="B45">Schultheiss et&#xa0;al., 2011</xref>). The coring autoclave on display holds a 3.5-meter core sample. In addition, the refrigerated storage tank contains 16 individual one-meter storage chambers. The refrigerated infrastructure container is equipped with chillers, heat exchangers, pumps, and manifolds (not shown). Additionally, the lab&#x2019;s central area contains the motor control electronics and computer equipment.</p>
<fig id="f1" position="float">
<label>Figure&#xa0;1</label>
<caption>
<p>Scale diagram of PCATS (<xref ref-type="bibr" rid="B45">Schultheiss et&#xa0;al., 2011</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g001.tif">
<alt-text content-type="machine-generated">Diagram illustrating a laboratory setup divided into three sections: refrigerated infrastructure with a manipulator, central laboratory featuring a cutter, analysis sensors, and an autoclave with a core, and refrigerated storage containing multiple storage chambers.</alt-text>
</graphic>
</fig>
<p>The PCATS system performs multiple roles in pressure coring operations, streamlining coring logistics through rapid autoclave recycling while also delivering fundamental scientific measurements like P-wave velocity data. PCATS can remove cores up to 3.5 meters in length from a matched pressure coring device (Fugro FPC &amp; FRPC, Aumann HPTC and Hybrid PCS) while maintaining the original temperature and pressure (up to 35 MPa) of the formation, perform nondestructive analysis, cut core subsamples and transfer them into other chambers (<xref ref-type="bibr" rid="B16">Holland et&#xa0;al., 2008</xref>). The nondestructive analysis suite comprises gamma-ray density assessment, P-wave velocity measurement, along with both 2D radiography and 3D CT scanning. These methods yield scientifically valuable data essential for subsample selection. Depending on the sediment type, a variety of cutting techniques can be used to process the core into a &#x201c;whole round&#x201d; subsample (<xref ref-type="bibr" rid="B44">Schultheiss et&#xa0;al., 2008</xref>). Depending on requirements, cut cores may be placed in either basic storage chambers (10&#xa0;cm - 3.5&#xa0;m capacity) or dedicated instrumented cells configured for advanced geotechnical and rock physics measurements. Samples stored in storage chamber can be re-entered into the PCATS system for in-depth analysis or preparation of subsamples, depending on the needs of the experiment. <xref ref-type="fig" rid="f2">
<bold>Figure&#xa0;2</bold>
</xref> shows the photo of PCATS central laboratory.</p>
<fig id="f2" position="float">
<label>Figure&#xa0;2</label>
<caption>
<p>Photo of PCATS central laboratory.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g002.tif">
<alt-text content-type="machine-generated">A technician in a blue uniform and red hard hat operates equipment inside an industrial control room. Several computer screens display data and graphs. The room is filled with pipes and machinery.</alt-text>
</graphic>
</fig>
<p>
<xref ref-type="table" rid="T1">
<bold>Table&#xa0;1</bold>
</xref> shows the technical parameters of PCATS. Although the PCATS system is fully functional, existing articles do not explicitly mention the adaptability limitations of core processing and performance stability under high-pressure and low-temperature conditions.</p>
<table-wrap id="T1" position="float">
<label>Table&#xa0;1</label>
<caption>
<p>Technical parameters of PCATS.</p>
</caption>
<table frame="hsides">
<thead>
<tr>
<th valign="middle" align="center">Name</th>
<th valign="middle" align="center">Parametric</th>
<th valign="middle" align="center">Name</th>
<th valign="middle" align="center">Parametric</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="middle" align="center">Compatible coring tools</td>
<td valign="middle" align="center">Fugro FPC &amp; FRPC, Aumann HPTC and Hybrid PCS</td>
<td valign="middle" align="center">Rotational core motion</td>
<td valign="middle" align="center">&#xb1; 0.1&#x2da;</td>
</tr>
<tr>
<td valign="middle" align="center">Maximum core length</td>
<td valign="middle" align="center">3.5 m</td>
<td valign="middle" align="center">Gamma density sensor</td>
<td valign="middle" align="center">137-Cs 10 mCi source and NaI detector</td>
</tr>
<tr>
<td valign="middle" align="center">Core liner specifications</td>
<td valign="middle" align="center">OD up to 63&#xa0;mm, liner thickness up to 4 mm</td>
<td valign="middle" align="center">P-wave velocity sensor</td>
<td valign="middle" align="center">Geotek 250 kHz transducers</td>
</tr>
<tr>
<td valign="middle" align="center">Maximum operating pressure</td>
<td valign="middle" align="center">35 MPa</td>
<td valign="middle" align="center">X-ray imaging</td>
<td valign="middle" align="center">130 kV microfocal source &amp; 14-bit digital flat-panel detector (1920 x 1536 pixel); image resolution 100 microns</td>
</tr>
<tr>
<td valign="middle" align="center">Operating temperature range</td>
<td valign="middle" align="center">4-30&#x2da;C</td>
<td valign="middle" align="center">System length</td>
<td valign="middle" align="center">18.3 m (3 x 20-foot standard shipping containers)</td>
</tr>
<tr>
<td valign="middle" align="center">Linear core motion</td>
<td valign="middle" align="center">&#xb1; 0.1 mm</td>
<td valign="middle" align="center">System weight</td>
<td valign="middle" align="center">approx 24 tons</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s2_2">
<label>2.2</label>
<title>Mini-PCATS</title>
<p>Mini-PCATS (<xref ref-type="fig" rid="f3">
<bold>Figure&#xa0;3</bold>
</xref>), a compact and streamlined adaptation of the PCATS system, maintains full compatibility with standard pressure core storage chambers and third-party equipment utilizing Geotek&#x2019;s flange-clamp interface. At the pressure of 35 MPa, mini-PCATS can take core sections up to 1.2 meters from the storage chamber and accurately slice them along with the plastic liner tubing, which can then be transferred to test units such as the K<sub>0</sub> Permeameter and PCATS Triaxial for determination of physical parameters (<xref ref-type="bibr" rid="B46">Schultheiss et&#xa0;al., 2017</xref>). For samples intended to be tested in devices such as the PCATS Triaxial or the K<sub>0</sub> Penetrometer, precise control of their geometry is essential. Mini-PCATS uses a new design for cutting core samples that consists of a rolling circular blade for cleanly cutting plastic liners and a reciprocating saw blade for cutting sediment/rock samples. The new technology allows for &#x201c;square&#x201d; end cutting of samples as opposed to older guillotine system that occasionally caused brittle samples to break along the laminar surfaces. By integrating an X-ray system into the mini-PCATS, the operator can visually confirm the position of the sample ends as well as check the quality of the cuts and thus make sample selection decisions. The Mini-PCATS system is compact, portable, and compatible with standard pressure chambers, but existing literature does not mention the imaging resolution limitations of X-rays in complex rock types.</p>
<fig id="f3" position="float">
<label>Figure&#xa0;3</label>
<caption>
<p>Picture of mini-PCATS.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g003.tif">
<alt-text content-type="machine-generated">Two individuals are in a lab setting. One is working at a computer with dual monitors displaying data, and the other is taking notes on a clipboard beside machinery labeled &#x201c;Geotek.&#x201d; Industrial equipment and gauges are visible, and a warning light system indicating X-ray status is mounted above.</alt-text>
</graphic>
</fig>
<p>
<xref ref-type="fig" rid="f4">
<bold>Figure&#xa0;4</bold>
</xref> shows the schematic diagram of the Mini-PCATS system, which primarily consists of a pressure housing, core movement mechanism, core cutter, P-wave sensors, X-ray imaging, and a ball valve. Compared to the PCATS system, the Mini-PCATS system features a newly designed core cutter that is more suitable for precise core cutting in laboratory settings; the PCATS system, on the other hand, is designed for rapid core cutting on drilling vessels. The Mini-PCATS system can be connected to a custom pressure cell via Geotek&#x2019;s flange-clamp and feed core subsamples into it. The custom pressure cell is then connected to third-party equipment and feeds the core subsamples into the test chamber to complete third-party testing of the core.</p>
<fig id="f4" position="float">
<label>Figure&#xa0;4</label>
<caption>
<p>Schematic diagram of mini-PCATS.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g004.tif">
<alt-text content-type="machine-generated">Diagram of a system showing core movement through a pressure housing towards a core cutter. P-wave sensors and X-ray imaging are labeled near the core cutter, with a ball valve at the end.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s2_3">
<label>2.3</label>
<title>HYACINTH (development of HYACE tools in new tests on hydrates)</title>
<p>The HYACINTH system, shown in <xref ref-type="fig" rid="f5">
<bold>Figure&#xa0;5</bold>
</xref>, was developed to explore the response behavior of sedimentary formations in pressure environments up to 250&#xa0;bar (25 MPa) (<xref ref-type="bibr" rid="B43">Schultheiss et&#xa0;al., 2009</xref>). The system operates by acquiring pressure-maintained core samples from boreholes, enabling their retrieval, subdivision, and laboratory analysis under controlled conditions. A &#x201c;quick fit&#x201d; flange has been designed to facilitate the direct tandem connection of different chambers without the need for threaded pipe fittings or flange nuts. In this way, the pressure chambers can be quickly connected to each other without requiring rotation. For transferring cores between pressure chambers, a dedicated Manipulator Chamber has been developed. It operates using two methods: actively pushing cores between chambers or mechanically latching onto samples to withdraw them into a destination chamber. The pressure chambers in the system, which have independent pressure maintaining function, are equipped with specially developed large diameter (65mm) ball valves to ensure that the cores obtained by FPC or HRC can be flexibly transferred between the chambers (<xref ref-type="bibr" rid="B42">Schultheiss et&#xa0;al., 2006</xref>). By using an inverted cone seal interface at the end of the chamber opposite to the ball valve to connect the manipulator, the dual optimization of equipment light weighting and cost control is achieved. The HYACINTH system utilizes inverted cone seals to achieve weight reduction and cost control, but its maximum working pressure is lower than that of the PCATS system, and existing articles do not clearly state its compatibility with extra-long cores (such as 3.5 meters).</p>
<fig id="f5" position="float">
<label>Figure&#xa0;5</label>
<caption>
<p>Diagram of the HYACINTH transfer system (<xref ref-type="bibr" rid="B42">Schultheiss et&#xa0;al., 2006</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g005.tif">
<alt-text content-type="machine-generated">Cross-sectional diagram of a core sampling device. Labeled parts include: push rod, quick-release clamp, core latch, technical portion of core, shear blades, geological portion of core, ball valve. Sections identified are manipulator chamber, shear transfer chamber, and storage chamber or tool autoclave. Arrows indicate directional movement.</alt-text>
</graphic>
</fig>
<p>The initial transfer operation aimed to remove the Catch Assembly from the autoclave and subsequently disassemble it into its two constituent parts: the technical module and geological sample component. To realize this function, the system consists of an autoclave in a series circuit with a Shear Transfer Chamber (STC) and a Manipulator (<xref ref-type="fig" rid="f6">
<bold>Figure&#xa0;6</bold>
</xref>). Once the pressure is equalized, the Manipulator is extended through the STC to the autoclave to engage the core. The core is then pumped back to the STC where the technical and geological sections of the Catch Assembly are separated by cutting the liner. During this process, the original coring autoclave is isolated and removed and replaced with a logging or storage chamber (<xref ref-type="bibr" rid="B3">Anders and M&#xfc;ller, 2008</xref>). Following pressure equalization, the Manipulator advances the geological core segment into either the logging or storage chamber, while retracting the remaining technical portion (still engaged with the Manipulator) back into the STC. The ball valves of the logging or storage chamber were closed and removed to allow scientific measurements to be made, while the technical component of the core was recovered and prepared for subsequent coring missions (<xref ref-type="bibr" rid="B33">Parkes et&#xa0;al., 2009</xref>). Since the FPC and HRC cores differ slightly in diameter, the STC is equipped with two specialized cutting boxes&#x2014;each designed for the specific core dimensions. These boxes can be rapidly swapped thanks to the quick-release butt flange clamp system.</p>
<fig id="f6" position="float">
<label>Figure&#xa0;6</label>
<caption>
<p>Workflow diagram of the HYACINTH transfer system.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g006.tif">
<alt-text content-type="machine-generated">Diagram showing seven stages (A to G) of a core sampling system. Each stage features components like manipulators, quick clamps, cutting blades, ball valves, and chambers for a technical and geological core. Accompanying equipment includes a shear transfer chamber, FPC or IIRC corer autoclave, MSCI-P, X-ray unit, P-wave and gamma-ray density measurement chamber, and HYACINTH storage chamber. Stages progress from start (A) to catch (B), retract (C), log (D), cut (E), push (F), and store (G).</alt-text>
</graphic>
</fig>
</sec>
<sec id="s2_4">
<label>2.4</label>
<title>A Pressure-retained transfer system</title>
<p>Zhejiang University, together with Dalian University of Technology and Technology and Guangzhou Marine Geological Survey, has developed a pressure-retained transfer system for natural gas hydrate cores with the financial support of the National High Technology Research and Development Program (863 Program) (<xref ref-type="bibr" rid="B13">Gao et&#xa0;al., 2020</xref>), as shown in <xref ref-type="fig" rid="f7">
<bold>Figure&#xa0;7</bold>
</xref>. The system is mainly adapted to the Long Gravity-Piston Corer (<xref ref-type="bibr" rid="B6">Chen et&#xa0;al., 2013</xref>), with a maximum working pressure of 20 MPa, which can handle cores with a length of 1.2&#xa0;m and an external diameter of 67&#xa0;mm, and the pressure change in the transferring process is no more than 20%. The system has the functions of core transfer, core cutting, P-wave velocity measurement and pressure core storage (<xref ref-type="bibr" rid="B7">Chen et&#xa0;al., 2019</xref>). The sub-sampler can be connected to the core storage chamber to obtain sub-samples of 200&#xa0;mm in length and 20&#xa0;mm in external diameter, which are mainly used for X-ray computed tomography to obtain the skeletal structure and pore storage pattern of natural gas hydrate formations. In 2016, the research team selected two test sites in the South China Sea for gas hydrate exploration areas to carry out systematic sea trials, including Site 1 with a water depth of 2,264 meters and Site 2 with a water depth of 1,130 meters (<xref ref-type="bibr" rid="B14">Gao et&#xa0;al., 2019</xref>). The system is designed specifically for natural gas hydrates and can obtain 200&#xa0;mm &#xd7; 20&#xa0;mm subsamples for CT structural analysis, but its working pressure and core length are lower than those of the PCATS system.</p>
<fig id="f7" position="float">
<label>Figure&#xa0;7</label>
<caption>
<p>Documentary images from the 2016 sea trial.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g007.tif">
<alt-text content-type="machine-generated">Laboratory equipment is set up on the deck of a ship with the ocean in the background. A long metallic structure is mounted on a frame, and various tools and wires are visible.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s2_5">
<label>2.5</label>
<title>A pressure core transfer and testing system</title>
<p>Under the support of the National Key R&amp;D Program and the Zhejiang Key R&amp;D Program, Zhejiang University has developed a pressure core transfer and testing system (<xref ref-type="bibr" rid="B36">Ren et&#xa0;al., 2018</xref>). As shown in <xref ref-type="fig" rid="f8">
<bold>Figure&#xa0;8</bold>
</xref>, the system is installed in three 6-meter containers, with the left container as the refrigeration facility room, which can maintain the low-temperature environment required during the test process; the middle container as the control room, which is used by the test personnel to operate the control system and the testing device (<xref ref-type="bibr" rid="B61">Zhang et&#xa0;al., 2018</xref>); and the right container as the cold storage room, which is used as the docking place of the coring tools and the core storage chambers (<xref ref-type="bibr" rid="B60">Zhang et&#xa0;al., 2019</xref>). The system can be matched with the drilling tools of seabed drilling rig or drillship to transfer a 4-meter-long core to the core storage chamber under the pressure of 35 MPa, and complete the core cutting, X-ray computed tomography, and P-wave velocity measurement of the core in the process (<xref ref-type="bibr" rid="B35">Ren et&#xa0;al., 2020</xref>). The system is compatible with a variety of coring tools and can handle 4-meter-long cores, but the efficiency of the system in handling non-homogeneous cores is not specified.</p>
<fig id="f8" position="float">
<label>Figure&#xa0;8</label>
<caption>
<p>Pressure core transfer and testing system developed by Zhejiang University.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g008.tif">
<alt-text content-type="machine-generated">Industrial setup with labeled components. Left to right: pressure maintenance and temperature control unit, core transfer unit, core cutting unit, test and analysis system, and drilling tool docking unit. The control system is labeled above.</alt-text>
</graphic>
</fig>
<p>From March to May 2021, the system was carried on board the vessel &#x201c;Marine Geology II&#x201d; for sea trials in the South China Sea, during which the transfer and testing of cores from 6 stations were completed, and the results showed that the temperature of the cores could be maintained in the range of 3.0-4.5 &#xb0;C, the fluctuation of pressure was less than 3.8%. Non-destructive cutting of the core can be carried out with an error of 2mm or less (<xref ref-type="bibr" rid="B62">Zhu et&#xa0;al., 2022</xref>).</p>
</sec>
<sec id="s2_6">
<label>2.6</label>
<title>Summary</title>
<p>The preceding sections have introduced five systems for the processing and analysis of natural gas hydrate pressure cores. To facilitate readers&#x2019; comprehensive evaluation of the performance and application scenarios of different systems, this section compares the core indicators of the five systems (<xref ref-type="table" rid="T2">
<bold>Table&#xa0;2</bold>
</xref>). The comparison dimensions cover working pressure, core size, compatible equipment, and core functions, with a focus on the technical differences between the systems in terms of <italic>in-situ</italic> pressure retention capability and laboratory analysis compatibility.</p>
<table-wrap id="T2" position="float">
<label>Table&#xa0;2</label>
<caption>
<p>Comparison of key indicators for core processing systems.</p>
</caption>
<table frame="hsides">
<thead>
<tr>
<th valign="middle" align="center">Name</th>
<th valign="middle" align="center">Maximum operating pressure/MPa</th>
<th valign="middle" align="center">Maximum core length/m</th>
<th valign="middle" align="center">Operating temperature Range/&#xb0;C</th>
<th valign="middle" align="center">Maximum core diameter/mm</th>
<th valign="middle" align="center">Compatible coring tools</th>
<th valign="middle" align="center">Testing methods</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="middle" align="center">PCATS</td>
<td valign="middle" align="center">35</td>
<td valign="middle" align="center">3.5</td>
<td valign="middle" align="center">4-30&#xb0;C</td>
<td valign="middle" align="center">63</td>
<td valign="middle" align="center">Fugro FPC &amp; FRPC, Aumann HPTC and Hybrid PCS</td>
<td valign="middle" align="center">Gamma density sensor<break/>P-wave velocity sensor<break/>X-ray imaging</td>
</tr>
<tr>
<td valign="middle" align="center">Mini-PCATS</td>
<td valign="middle" align="center">35</td>
<td valign="middle" align="center">1.2</td>
<td valign="middle" align="center">/</td>
<td valign="middle" align="center">63</td>
<td valign="middle" align="center">/</td>
<td valign="middle" align="center">P-wave velocity sensor<break/>X-ray imaging</td>
</tr>
<tr>
<td valign="middle" align="center">HYACINTH</td>
<td valign="middle" align="center">25</td>
<td valign="middle" align="center">/</td>
<td valign="middle" align="center">/</td>
<td valign="middle" align="center">65</td>
<td valign="middle" align="center">FPC or HRC</td>
<td valign="middle" align="center">Gamma density sensor<break/>P-wave velocity sensor<break/>X-ray imaging</td>
</tr>
<tr>
<td valign="middle" align="center">A Pressure-retained Transfer System</td>
<td valign="middle" align="center">20</td>
<td valign="middle" align="center">1.2</td>
<td valign="middle" align="center">Environmental temperature</td>
<td valign="middle" align="center">67</td>
<td valign="middle" align="center">Long Gravity-Piston Corer</td>
<td valign="middle" align="center">P-wave velocity sensor<break/>X-ray imaging</td>
</tr>
<tr>
<td valign="middle" align="center">A Pressure Core Transfer and Testing System</td>
<td valign="middle" align="center">35</td>
<td valign="middle" align="center">4.0</td>
<td valign="middle" align="center">2-30&#xb0;C</td>
<td valign="middle" align="center">61</td>
<td valign="middle" align="center">Coring tools for subsea drilling rigs and drilling vessels</td>
<td valign="middle" align="center">P-wave velocity sensor<break/>X-ray imaging</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
</sec>
<sec id="s3">
<label>3</label>
<title>Development and application of tools for analyzing pressure core samples</title>
<p>The safe handling of large quantities of high-pressure, cryogenically preserved cores or core segments (either temporarily or for long periods of time) is always a difficult logistical challenge in gas hydrate pressure-holding core drilling operations. In order to obtain a large amount of formation information, a variety of tools for core analysis have been developed. This chapter describes the current research progress in gas hydrate reservoir core analysis techniques and summarizes the functional advantages and limitations of several major gas hydrate reservoir core analysis tools.</p>
<sec id="s3_1">
<label>3.1</label>
<title>Field depressurization laboratory</title>
<p>Quantitative depressurization/degassing experiments are typically conducted in small storage chambers (<xref ref-type="fig" rid="f9">
<bold>Figure&#xa0;9</bold>
</xref>) on short sections of cores up to 35&#xa0;cm in length and are performed in a controlled manner to accurately quantify the total amount of each phase of hydrocarbon gas in the subsample, including gas hydrates. This makes it possible to determine the concentration (saturation) of gas hydrates and the overall distribution of gas hydrates in the sedimentary sequence. Step-by-step pressure reduction is used to gradually reduce the pressure in the subsample chamber using a manifold, and the gas-liquid mixture released under each pressure gradient is quantitatively collected in the bubble metering and gas collection chambers (<xref ref-type="bibr" rid="B46">Schultheiss et&#xa0;al., 2017</xref>). Gas composition analysis is carried out throughout the depressurization process and is used to determine the component content of gas hydrates in the sediments. The presence of natural gas hydrates can be verified both kinetically and thermodynamically from pressure-time-volume relationships in depressurization experiments. Kinetic evidence is usually manifested as a pressure rebound after the bucking step, i.e., the pressure increases with time. The thermodynamic criterion for hydrate endowment is reflected in the pressure-volume relationship of the degassing process, i.e., the system pressure remains steady while the gas release volume continues to increase during the predicted hydrate stabilization pressure interval.</p>
<fig id="f9" position="float">
<label>Figure&#xa0;9</label>
<caption>
<p>Depressurization laboratory for high-throughput quantitative core (<xref ref-type="bibr" rid="B46">Schultheiss et&#xa0;al., 2017</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g009.tif">
<alt-text content-type="machine-generated">A person in blue coveralls operates equipment in a laboratory setting. They are surrounded by several cylindrical machines and monitoring screens. The environment is filled with tubes and control panels, indicating a technical or scientific process underway.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s3_2">
<label>3.2</label>
<title>PCATS triaxial system</title>
<p>The PCATS Triaxial system (<xref ref-type="fig" rid="f10">
<bold>Figure&#xa0;10</bold>
</xref>) represents a specialized mechanical testing platform capable of conducting geotechnical analyses across both small and large strain regimes on pressure-preserved core specimens of soils or rocks, while simultaneously facilitating direct permeability flow measurements. After the samples were cut in the PCATS system, they were transferred to the PCATS triaxial for testing while maintaining a maximum of 25 MPa <italic>in-situ</italic> hydrostatic pressure (<xref ref-type="bibr" rid="B47">Schultheiss et&#xa0;al., 2014</xref>). Consequently, samples of gas hydrate-bearing sediments or rocks can be taken from deep in the earth and transferred to sophisticated mechanical testing instruments under near-<italic>in-situ</italic> conditions, while keeping the gas hydrate in its stabilization field (<xref ref-type="bibr" rid="B34">Priest et&#xa0;al., 2015</xref>). The complete triaxial pressure control within the system enables acquisition of reliable geomechanical properties and accurate permeability measurements. A viewing window in the main pressure housing allows direct observation of the delicate process of transferring the sample from the plastic liner to the thin rubber membrane.</p>
<fig id="f10" position="float">
<label>Figure&#xa0;10</label>
<caption>
<p>Schematic diagram PCATS Triaxial system.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g010.tif">
<alt-text content-type="machine-generated">Diagram and photograph of a triaxial testing machine. The diagram labels parts such as the top manipulator, test cell, load cell, top platen, and servo drives. The photograph shows the physical machine with its various mechanical components and setup, consistent with the diagram's labels.</alt-text>
</graphic>
</fig>
<p>The test chamber features modular drive heads that enable multiple testing modalities: a resonant column configuration for small-strain characterization and a conventional triaxial setup for large-deformation analysis. The small strain resonance test uses an electromagnetic drive to apply a torsional load to the specimen to determine the resonance frequency of the specimen-drive system (<xref ref-type="bibr" rid="B45">Schultheiss et&#xa0;al., 2011</xref>). Based on the calibration method and elastic wave theory, the shear modulus of the specimen and the shear wave propagation velocity can be calculated from this. The sample&#x2019;s large-deformation response is characterized through conventional triaxial testing methodology, enabling comprehensive determination of stress-strain behavior under controlled loading conditions. An incremental uniaxial load is applied to the specimen and the change in axial deformation with load is monitored. For permeability testing, the differential pressure method is used, which is realized by measuring the fluid flow rate (<xref ref-type="bibr" rid="B23">Liang et&#xa0;al., 2017</xref>).</p>
</sec>
<sec id="s3_3">
<label>3.3</label>
<title>K<sub>0</sub> Permeameter</title>
<p>Complementing the PCATS triaxial system, a newly developed test cell was specifically designed to acquire permeability data under simulated <italic>in-situ</italic> K<sub>0</sub> stress conditions. This K<sub>0</sub> system (<xref ref-type="fig" rid="f11">
<bold>Figure&#xa0;11</bold>
</xref>) has a simpler and lower cost structure that improves sample handling efficiency and maintains <italic>in-situ</italic> hydrostatic pressure of up to 35 MPa throughout sample cutting and transfer, enabling direct core transfer to the K<sub>0</sub> permeameter. The test cell enables comprehensive evaluation of both consolidation properties and permeability characteristics of the samples. A flexible rubber sleeve is used to wrap the specimen, and the effective stress is applied to restore the <italic>in-situ</italic> stress state of the formation by independently controlling the perimeter and pore pressures. Pressure core samples (25-80&#xa0;mm length) extracted from PCTB storage can be precisely sectioned using either PCATS or mini-PCATS, then transferred to the K<sub>0</sub> permeameter test cell. The cell features an integrated ball valve for isolation, enabling disconnection from mini-PCATS and subsequent integration with the Axial Load Transfer System (ALTS) (<xref ref-type="bibr" rid="B46">Schultheiss et&#xa0;al., 2017</xref>). This combined system is capable of extruding the sample from the liner tube into a flexible rubber sleeve within the test cell. After completing the sample extrusion into the rubber sleeve at a constant pressure, a perimeter pressure and vertical load (via ALTS) can be applied as needed to return the sample to its <italic>in-situ</italic> stress state prior to permeability testing. Permeability testing is performed by direct seepage measurements through the porous stone at the top and bottom of the sample. The K<sub>0</sub> permeameter test chamber interfaces with the ALTS, which stabilizes the sample in horizontal position during transfer operations before reorienting vertically for analytical procedures.</p>
<fig id="f11" position="float">
<label>Figure&#xa0;11</label>
<caption>
<p>Schematic diagram of the K<sub>0</sub> system (<xref ref-type="bibr" rid="B46">Schultheiss et&#xa0;al., 2017</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g011.tif">
<alt-text content-type="machine-generated">A person adjusts a complex, industrial-looking machine composed of interconnected metal components, valves, and gauges mounted on a wheeled frame. The setup suggests a laboratory or industrial application.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s3_4">
<label>3.4</label>
<title>The HYACINTH subsampling system</title>
<p>The HYACINTH subsampling system, which allows subsampling under pressure and then relocating subsamples to dedicated analysis chambers for subsequent testing and experimentation, is designed to meet varied research requirements, the technology supports applications ranging from gas chromatography and pore-water extraction to biological incubation and geomechanical testing. A subsampling tool such as the one shown in <xref ref-type="fig" rid="f12">
<bold>Figure&#xa0;12</bold>
</xref> allows for a 10-cm-long complete core segment to be intercepted from anywhere in a 1-m-long FPC or HRC core and transferred to a small core segment storage compartment (the precise location of this sub-segment can be determined after analyzing logging data from cores acquired under pressure-holding conditions). <xref ref-type="fig" rid="f12">
<bold>Figure&#xa0;12</bold>
</xref> illustrates the process of transferring pressure core subsamples to the microbiology experiments chamber: (A) the Core Section Chamber (CSC) maintains pressure integrity in whole-round core segments while providing fluid/gas connections to adjacent analysis chambers; (B) following chamber pressurization, the CSC&#x2019;s large-bore ball valve is actuated to permit transfer of core segments into the extrusion tube via mechanical displacement, and the rotating cutter obtains an axial subsample and pushes it into the microbiology chamber; and (C) a sterile subsample is obtained for microbiology experiments  (<xref ref-type="bibr" rid="B42">Schultheiss et&#xa0;al., 2006</xref>).</p>
<fig id="f12" position="float">
<label>Figure&#xa0;12</label>
<caption>
<p>Diagram of subsampling tool (<xref ref-type="bibr" rid="B42">Schultheiss et&#xa0;al., 2006</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g012.tif">
<alt-text content-type="machine-generated">Diagram of a core sectioning system with labeled components. Panel A shows the axial sub-core extrusion chamber and associated parts. Panel B details the inner workings, including a camera, minipusher, and rotating cutter. Panel C illustrates an aseptically collected sub-sample in a pressurized chamber. Arrows and labels indicate flow and chamber functions, such as the microbiological experimental chamber and sub-core positioning.</alt-text>
</graphic>
</fig>
</sec>
<sec id="s3_5">
<label>3.5</label>
<title>PCCT (pressure core characterization tools)</title>
<p>Developed by Georgia Tech, the Pressure Core Characterization Tools comprise a suite of instrumented pressure vessels that were operationally deployed in Sapporo, Japan (January 2013) to characterize gas hydrate-bearing pressure cores from the 2012 Eastern Nankai Trough campaign. The system measured key properties including geomechanical, hydraulic, electrical, and biological parameters. The manipulator (MAN) is a longitudinal positioning device capable of gripping and transferring cores between chambers under the required temperature and pressure conditions (<xref ref-type="bibr" rid="B38">Santamarina et&#xa0;al., 2012a</xref>). <xref ref-type="fig" rid="f13">
<bold>Figure&#xa0;13</bold>
</xref> illustrates the standard operating procedure: the sample is first extracted from the storage compartment to the manipulator, which then pushes it to the test chamber. The length (L<sub>man</sub>) of the MAN (including the temporary storage chamber) is designed as 3.5 times the core length (L<sub>core</sub>). For a standard core length of 1.2 meters, the system uses a telescopic screw drive mechanism (stepper motor driven) with a stroke of 2.6 meters and a positioning resolution of better than 1&#xa0;mm (<xref ref-type="bibr" rid="B51">Yamamoto, 2015</xref>). A detachable flange clamp connection is used to interface with the 1.3-meter temporary storage chamber, equipped with a visualization window for continuous monitoring of the manipulator&#x2019;s positioning. The 1.2-meter core handled supports segmental cutting. The cutting tool (CUT) is configured with a linear or circular saw blade in a clamped chamber for saw-based machining that results in flat end surfaces and minimizes sample disturbance. The CUT can be mounted in tandem between the MAN and other test or storage chambers as required (<xref ref-type="fig" rid="f13">
<bold>Figures&#xa0;13d, e</bold>
</xref>).</p>
<fig id="f13" position="float">
<label>Figure&#xa0;13</label>
<caption>
<p>Pressure core manipulation (<xref ref-type="bibr" rid="B39">Santamarina et&#xa0;al., 2012b</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g013.tif">
<alt-text content-type="machine-generated">Sequential diagrams labeled A to E illustrate a mechanism with components including a screw chamber, temporary storage chamber, and a ball valve. A C-clamp holds the specimen within a plastic liner. Diagrams show a manipulator alongside varying configurations with a cutter and characterization tool, indicating procedural steps for specimen handling within a storage chamber system.</alt-text>
</graphic>
</fig>
<p>Instrumented Pressure Testing Chamber (IPTC) (<xref ref-type="fig" rid="f14">
<bold>Figure&#xa0;14A</bold>
</xref>). The chamber was specifically designed to enable fluid sampling while simultaneously acquiring geophysical and geotechnical data, including compressional/shear wave velocities, undrained shear strength, bulk electrical conductivity, and <italic>in-situ</italic> temperature profiles. The cylindrical chamber is equipped with two sets of four radially symmetrical port pairs. The first set of ports is used to drill holes (8&#xa0;mm in diameter) in the plastic liner so that the contact probes in the subsequent ports can be inserted into the sample (<xref ref-type="bibr" rid="B59">Yun et&#xa0;al., 2006</xref>). The IPTC is connected to the MAN and the extension chamber on each side to support arbitrary point measurements over the full length of the core during testing. The design of eight inspection interfaces makes it an ideal multifunctional module for conducting production experiments related to reservoir calibration modeling.</p>
<fig id="f14" position="float">
<label>Figure&#xa0;14</label>
<caption>
<p>Schematic diagrams of characterization chambers (Santamarina et&#xa0;al., 2012).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g014.tif">
<alt-text content-type="machine-generated">Diagram of a drilling apparatus showing components like a manipulator, extension chamber, and specimen holder in section A. Sections B and C display plungers, while section D includes a sapphire window and sampler. Section E features a self-drilling thermocouple, gas trap, and water trap mechanisms. Labels identify each part.</alt-text>
</graphic>
</fig>
<p>Effective Stress Chamber (ESC) (<xref ref-type="fig" rid="f14">
<bold>Figure&#xa0;14B</bold>
</xref>). The ESC can precisely control the temperature and pressure to reconstruct the <italic>in-situ</italic> effective stress environment of the sedimentary layer. During the experiment, the sample is sealed by a flexible membrane and subjected to an effective stress of 3MPa, which realistically simulates the <italic>in-situ</italic> stress state generated by the weight of the overlying strata. Small-strain stiffness, compressibility, hydraulic conductivity, volume contraction upon dissociation, and hydrate saturation were measured. Georgia Tech completed the development and laboratory testing of the equipment in 2006 under a Joint Oceanographic Institutions (JOI) grant, and its first field application was implemented by the Korea Institute of Geoscience &amp; Mineral Resource (KIGM) in conjunction with Geotek (<xref ref-type="bibr" rid="B9">Dai et&#xa0;al., 2017</xref>).</p>
<p>Direct Shear Chamber (DSC) (<xref ref-type="fig" rid="f14">
<bold>Figure&#xa0;14C</bold>
</xref>). The core of the DSC is a thick-walled stainless-steel ring that shears the center third of the specimen by propulsion. The DSC system incorporates three key components: (1) a stress-recovery piston (functionally equivalent to the ESC system), (2) a liner capture mechanism that intercepts the plastic sleeve prior to specimen transfer into the shear chamber, and (3) a compact lateral actuator assembly that drives the side piston for ring displacement. The maximum shear displacement of the system is 15mm, which allows both peak shear strength and residual strength values to be obtained. Through DSC testing, the researchers determined the compressive deformation and shear strength of rock samples before and after hydrate decomposition in a 3 MPa <italic>in situ</italic> effective stress environment (<xref ref-type="bibr" rid="B40">Sapporo Scientific Team, 2013</xref>). The P-wave velocity and temperature were continuously monitored during the experiment, covering loading, shearing and dissociation stages. The DSC data were also utilized to study the creep deformation and volume shrinkage effects induced by dissociation.</p>
<p>Sub-sampling Tool for Bio-Studies (BIO) (<xref ref-type="fig" rid="f14">
<bold>Figure&#xa0;14D</bold>
</xref>). The BIO is disengaged from the system once the core section is loaded by the MAN, which allows for the contamination-free transfer of multiple samples to the bioreactor while maintaining the stable temperature and pressure of the hydrate and the addition of microbial nutrients via a high-pressure injection system (Santamarina et&#xa0;al., 2012). The operational protocol comprises four sequential steps: (1) displacement of liquids with nitrogen purging, (2) surface sterilization of core interfaces and chamber decontamination, (3) rotary coring extraction of sub-samples, and (4) transfer of specimens into pre-conditioned bio-reactors containing 10 mL of nutrient medium. All operations can be monitored in real time through the sapphire window. The bioreactor is easy to replace: simply close the dual ball valve system and disconnect the quick connect coupler. The unit allows the acquisition of a large number of samples from a single core section while maintaining <italic>in-situ</italic> hydrostatic pressure.</p>
<p>Controlled Depressurization Chamber (CDC) (<xref ref-type="fig" rid="f14">
<bold>Figure&#xa0;14E</bold>
</xref>). The CDC is designed to effectively protect core lithology characteristics and capture critical data during depressurization with minimal human resource requirements. This stand-alone device has a built-in drilling station to perforate the liner at selected locations in order to reduce the longitudinal expansion of the specimen. Gas pressure and temperature are continuously monitored by pressure transmitters and thermocouples. The system also integrates three sets of self-drilling thermocouples, which are pierced into the core during the depressurization process to obtain real-time temperature data inside the sediment. A 2-liter water collector and a 55-liter gas collector are configured in series at the outlet end of the pressure-reducing control needle valve to achieve quantitative collection of the output water and gas (<xref ref-type="bibr" rid="B58">Yun et&#xa0;al., 2010</xref>).</p>
</sec>
<sec id="s3_6">
<label>3.6</label>
<title>PNATs (the pressure core nondestructive analysis tools)</title>
<p>To address the challenge of hydrate dissociation under atmospheric conditions, researchers developed the TACTT system (<xref ref-type="fig" rid="f15">
<bold>Figure&#xa0;15</bold>
</xref>), enabling complete sample preparation and testing while preserving hydrate stability conditions. Typically, pressurized triaxial test cells use stainless steel chambers capable of withstanding tens of megapascals of pressure, but TACTT uses transparent acrylic chamber, which allow visual confirmation of the condition of the specimen, both in terms of specimen shape and the condition of the end face after the transport process. The triaxial chamber with the bottom observation window design realizes the visualization and monitoring of the pressure core. The hydraulic system provides up to 16 MPa of perimeter and pore pressure, and the control accuracy is maintained within &#xb1;0.01 MPa. The instrument is suitable for sample sizes from 50.8 to 53.6&#xa0;mm in diameter and less than 110&#xa0;mm in height. The system provides a maximum load of 100 kN and a measurement accuracy of one thousandth of the full scale (<xref ref-type="bibr" rid="B52">Yoneda et&#xa0;al., 2013</xref>; <xref ref-type="bibr" rid="B40">Sapporo Scientific Team, 2013</xref>).</p>
<fig id="f15" position="float">
<label>Figure&#xa0;15</label>
<caption>
<p>Schematic diagram of the TACTT system (<xref ref-type="bibr" rid="B53">Yoneda et&#xa0;al., 2015</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g015.tif">
<alt-text content-type="machine-generated">Diagram and photos of a cylindrical pressure vessel system. The diagram details components like a pressure core, syringe pump, conveyance chamber, and camera. Photos show a transparent acrylic cell and observation window, with equipment and cameras arranged around the vessel for monitoring.</alt-text>
</graphic>
</fig>
<p>All pressure cores, comprising sediment-filled plastic liners (ID = 53.6&#xa0;mm), were preserved in pressurized vessels maintaining <italic>in-situ</italic> conditions of 10 MPa and 4&#xb0;C (<xref ref-type="fig" rid="f16">
<bold>Figure&#xa0;16a</bold>
</xref>). During testing, the pressure cores with plastic liner tubes were extracted by the PNATs Manipulator (<xref ref-type="fig" rid="f16">
<bold>Figure&#xa0;16b</bold>
</xref>). (<xref ref-type="fig" rid="f16">
<bold>Figure&#xa0;16c</bold>
</xref>) shows a schematic of the pressure retaining core transfer process: (i) the storage chamber was docked with PNATs and fluidly pressurized to achieve pressure balance on both sides of the ball valve; (ii) the pressure was stabilized by a syringe pump after the ball valve was opened; (iii) a manipulator transferred the cores wrapped in plastic liner tubes to the temporary storage chamber at 10 MPa; (iv) the storage chamber&#x2019;s ball valve was shut; and (v) the cores and the liner tubes were cut into 10-cm standard segments using a diamond saw (<xref ref-type="bibr" rid="B54">Yoneda et&#xa0;al., 2017</xref>). After cutting, the 10-centimeter-long core segment is maintained in a purpose-built pressure vessel designed for core preservation. This pressure chamber holds the plastic liner and pushes only the intact pressure core sediment into the transfer chamber. After docking the transfer chamber to the TACTT, the sample is moved into the triaxial pressure chamber via a lift table. The core sample was eventually pushed into clear acrylic chamber-sealed sleeves with locator marks (<xref ref-type="fig" rid="f16">
<bold>Figure&#xa0;16d</bold>
</xref>). Friction between the core sidewalls and the sleeve is minimized by maintaining consistent pressure inside and outside the sleeve during the transfer process. The sample is equipped with porous metal filters integrated into both the top endcap and the base pedestal, which link it to the pore fluid pressure system. During testing, syringe pumps regulate the confining (cell) pressure, as well as the pore fluid pressures at the upper and lower ends, while the core is secured inside the sleeve and the endcaps are properly positioned. A vertical displacement transducer records the sample&#x2019;s axial deformation. Additionally, target markers placed on the sleeve (<xref ref-type="fig" rid="f16">
<bold>Figure&#xa0;16d</bold>
</xref>) are monitored via image analysis, achieving an accuracy of &#xb1;0.1 mm (&#xb1; 0.1% strain). This precision is more than adequate to detect even minor volumetric changes in the sample.</p>
<fig id="f16" position="float">
<label>Figure&#xa0;16</label>
<caption>
<p>Pressure core handling protocol <bold>(a)</bold> Visual documentation of pressurized storage chambers. <bold>(b)</bold> Preparation of specialized core manipulation equipment. <bold>(c)</bold> Pressure core transfer procedures. <bold>(d)</bold> Photograph of the process of transferring the pressure core into the sealing sleeve (<xref ref-type="bibr" rid="B55">Yoneda et&#xa0;al., 2019</xref>).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g016.tif">
<alt-text content-type="machine-generated">Detailed assembly of a storage and testing system, featuring labeled components such as storage chambers, manipulators, and triaxial cells. Panel (a) shows storage chambers of varying sizes. Panel (b) illustrates components like storage and temporary chambers, cutter, and manipulation parts. Panel (c) outlines procedural diagrams for extracting and observing samples, with detailed steps using ball valves and pressure chambers. An inset shows sediment within a plastic liner. Panel (d) depicts a triaxial cell setup with an accompanying diagram highlighting the piezo element and sealing mechanisms.</alt-text>
</graphic>
</fig>
<p>During June-July 2012, three pressure core technologies (PCATs, PCCTs, and PNATs) underwent field evaluation at the eastern Nankai Trough, assessing their performance in recovering gas hydrate-bearing sediments. These tests formed part of the MH21 program&#x2019;s 2012-2013 offshore methane hydrate production trials (<xref ref-type="bibr" rid="B51">Yamamoto, 2015</xref>). Historical pressure core test data are compiled in <xref ref-type="table" rid="T3">
<bold>Table&#xa0;3</bold>
</xref>.</p>
<table-wrap id="T3" position="float">
<label>Table&#xa0;3</label>
<caption>
<p>Previous pressure core-based element test results.</p>
</caption>
<table frame="hsides">
<thead>
<tr>
<th valign="middle" align="center">Name</th>
<th valign="middle" align="center">Hydrate saturation <italic>S</italic>
<sub>h</sub>
</th>
<th valign="middle" align="center">Testing type</th>
<th valign="middle" align="center">Value</th>
<th valign="middle" align="center">Conditions</th>
<th valign="middle" align="center">Manipulated by</th>
<th valign="middle" align="center">Testing Tool</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="middle" rowspan="3" align="center">Strength</td>
<td valign="middle" align="center">0, 0, 0</td>
<td valign="middle" align="center">Undrained Triaxial compression</td>
<td valign="middle" align="center">Peak strength <italic>q</italic>
<sub>max</sub> = 2.2, 5.1, 1.7 MPa</td>
<td valign="middle" align="center">2.6, 2.6, 0.57 MPa Initial effective confining stress</td>
<td valign="middle" align="center">PCATS</td>
<td valign="middle" align="center">PCATS Triaxial</td>
</tr>
<tr>
<td valign="middle" align="center">0.27</td>
<td valign="middle" align="center">Direct shear</td>
<td valign="middle" align="center">Shear strengh <italic>&#x3c4;</italic> = 2.2 MPa</td>
<td valign="middle" align="center">3.0 MPa effective vertical stress</td>
<td valign="middle" align="center">PCATS &amp; PCCTs</td>
<td valign="middle" align="center">DSC</td>
</tr>
<tr>
<td valign="middle" align="center">0.63</td>
<td valign="middle" align="center">Drained Triaxial compression</td>
<td valign="middle" align="center">Peak strength <italic>q</italic>
<sub>max</sub> = 8.4 MPa</td>
<td valign="middle" align="center">1.5 MPa effective confining stress</td>
<td valign="middle" align="center">PCATS &amp; PNATs</td>
<td valign="middle" align="center">TACTT</td>
</tr>
<tr>
<td valign="middle" align="center">Compressibility</td>
<td valign="middle" align="center">0.18, 0.74</td>
<td valign="middle" align="center">Vertical loading and unloading</td>
<td valign="middle" align="center">Compression index <italic>C</italic>
<sub>c</sub> = 0.608, 0.407</td>
<td valign="middle" align="center">Confined</td>
<td valign="middle" align="center">PCATS &amp; PCCTs</td>
<td valign="middle" align="center">ESC</td>
</tr>
<tr>
<td valign="middle" rowspan="2" align="center">Permeability</td>
<td valign="middle" align="center">0, 0.71</td>
<td valign="middle" align="center">Permeability test</td>
<td valign="middle" align="center">Water permeability k = 40-127 mD, 19 mD</td>
<td valign="middle" align="center">0.57-2.6, 2.9 MPa effective confining stress</td>
<td valign="middle" align="center">PCATS</td>
<td valign="middle" align="center">PCATS Triaxial</td>
</tr>
<tr>
<td valign="middle" align="center">0.18, 0.74</td>
<td valign="middle" align="center">Permeability test</td>
<td valign="middle" align="center">Water permeability k = 128 mD, 6.12 mD</td>
<td valign="middle" align="center">3.0 MPa effective confining stress</td>
<td valign="middle" align="center">PCATS &amp; PCCTs</td>
<td valign="middle" align="center">ESC</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s3_7">
<label>3.7</label>
<title>The fidelity triaxial test device</title>
<p>As shown in <xref ref-type="fig" rid="f17">
<bold>Figure&#xa0;17</bold>
</xref>, the fidelity triaxial test device developed by Zhejiang University includes a sample transfer system, a triaxial host system, support structures, a data acquisition system, a temperature control system, and a pressure pressurization system (<xref ref-type="bibr" rid="B57">Yuhong et&#xa0;al., 2025</xref>). The device can be adapted to a pressure core transfer and testing system developed by Zhejiang University for quasi-<italic>in-situ</italic> mechanical characterization of gas hydrate reservoirs. The temperature control system operates within a range of 0 to 4 &#xb0;C, ensuring the stability of the hydrate state and maintaining sediment mechanical behavior. The device accommodates sample dimensions of a diameter of &#x3c6;39 mm and a maximum length of 78&#xa0;mm. It can apply an axial force ranging from 2 to 80 kN and achieve a maximum loading confining pressure of 30 MPa. <xref ref-type="table" rid="T4">
<bold>Table&#xa0;4</bold>
</xref> shows main technical indicators of the fidelity triaxial test device.</p>
<fig id="f17" position="float">
<label>Figure&#xa0;17</label>
<caption>
<p>Schematic diagram of fidelity triaxial test device (Wang et&#xa0;al., 2025).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g017.tif">
<alt-text content-type="machine-generated">Laboratory equipment diagram labeled with components including a data acquisition system, sample transfer system, triaxial host system, pressure pressurization system, temperature control system, and support structures.</alt-text>
</graphic>
</fig>
<table-wrap id="T4" position="float">
<label>Table&#xa0;4</label>
<caption>
<p>Main technical indicators of the fidelity triaxial test device.</p>
</caption>
<table frame="hsides">
<thead>
<tr>
<th valign="middle" align="center">Technical indicators</th>
<th valign="middle" align="center">Parameters</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="middle" align="center">Sample diameter</td>
<td valign="middle" align="center">39 mm</td>
</tr>
<tr>
<td valign="middle" align="center">Sample length</td>
<td valign="middle" align="center">&#x2264;78 mm</td>
</tr>
<tr>
<td valign="middle" align="center">Maximum loaded envelope pressure</td>
<td valign="middle" align="center">30 MPa</td>
</tr>
<tr>
<td valign="middle" align="center">Axial force load range</td>
<td valign="middle" align="center">2~80 kN</td>
</tr>
<tr>
<td valign="middle" align="center">Strain measurement precision</td>
<td valign="middle" align="center">&#x2264;0.01 mm</td>
</tr>
<tr>
<td valign="middle" align="center">Temperature control range</td>
<td valign="middle" align="center">0~4 &#xb0;C</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s3_8">
<label>3.8</label>
<title>The field parameter test system for gas hydrate pressure holding core samples</title>
<p>The field parameter test system for gas hydrate pressure holding core samples developed by the College of Engineering of China University of Geosciences (Wuhan) is shown in <xref ref-type="fig" rid="f18">
<bold>Figure&#xa0;18</bold>
</xref>. The system is mainly composed of core capture and cutting unit, sampler pressure maintaining unit, core sample parameter testing unit, core sample storage unit, temperature and pressure maintaining unit, etc. The core sample parameter testing unit is in the shape of a hollow cylinder with a total length of 1.7&#xa0;m, including quick coupling, ball valve, temperature sensor, pressure sensor, acoustic wave probe, resistivity probe, strength probe, wave velocity meter, resistivity meter, and shear strength meter. Temperature sensors and pressure sensors are used to monitor the temperature and pressure inside the core sample parameter testing unit, respectively. Acoustic wave probes are symmetrically distributed to measure the P-wave velocity of hydrate core samples. Resistivity probes are distributed at the front and rear to measure the resistivity of hydrate core samples. Strength probes and shear strength meters are used to measure the shear strength of hydrate core samples. Before the core is transferred to the parameter testing unit with pressure, the testing probe is to be on the outside of the core pressure cylinder, and when the core reaches the testing position, the probe is to be pierced into the inside of the hydrate core from the outside for parameter testing. In order to rotate the core liner in a pressure-holding environment to ensure that the acoustic, resistivity, and shear strength probes are aligned with the appropriate measurement points, a core liner rotation and positioning device was designed. The monitoring component allows you to see if the test holes are aligned with the test points on the core liner.</p>
<fig id="f18" position="float">
<label>Figure&#xa0;18</label>
<caption>
<p>The schematic diagram of the field parameter test system for gas hydrate pressure holding core samples. 1-Step motor; 2-Screw; 3-&#x201d;C&#x201d; tube; 4-Quick coupling; 5-Grabber; 6-Water jacket; 7-Cutter; 8-Ball valve; 9-Safety valve; 10-Quick coupling; 11-Temperature sensor; 12-Pressure sensor; 13-Pressure tracking port; 14-Pressure tracking port; 15-Quick coupling; 16-Ball valve; 17-Core inner tube; 18-Core liner; 19-Core; 20-Quick coupling; 21-Ball valve; 22-Temperature sensor; 23-Sonic probe; 24-Resistivity probe; 25-Strength probe; 26-Pressure sensor; 27-Wave velocity meter; 28-Resistivity meter; 29-Cutting shear strength meter; 30-Quick coupling; 31-Ball valve; 32-Storage cylinder; 33-Temperature monitoring indicator; 34-Pressure monitoring indicator; 35-Data acquisition processor; 36- Thermostatic circulating water bath; 37-Double-cylinder constant-speed constant-pressure pump.</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fmars-12-1613955-g018.tif">
<alt-text content-type="machine-generated">Diagram and photo depicting a core sampling system. The diagram shows sections labeled as core capture and cutting unit, sampler pressure maintaining unit, core sample parameter testing unit, and core sample storage unit, with numbered components. Connected equipment includes a computer (labeled 35) and control units (labeled 36, 37). The photo presents the physical setup in a workshop, featuring long metal pipes and machinery placed on stands in alignment with the diagram's sections.</alt-text>
</graphic>
</fig>
</sec>
</sec>
<sec id="s4" sec-type="conclusions">
<label>4</label>
<title>Conclusions and prospects</title>
<p>Natural gas hydrate reservoir core pressure-holding transfer and testing systems are indispensable investigation equipment for hydrate resource exploration and exploitation. Although countries worldwide have developed various analytical tools for more than 20 years, they are still inadequate. With the arrival of the period of commercial exploitation of hydrate resources, the requirements for analytical tools are increasing. Integration, intelligence and diversification are the future development trends of hydrate core analysis tools, which aim to carry out more complex field tests and obtain reliable data and information. Therefore, the development prospects of gas hydrate reservoir core pressure-holding transfer and testing technology are discussed from the following aspects.</p>
<list list-type="order">
<list-item>
<p>Automatization. Pressure retention transfer and testing in the field is very important due to the impact of long-term pressure retention storage on the physical properties of gas hydrate reservoir cores. However, handling a large number of cores under high pressure and low temperature for a long period of time is always a challenge due to the complexity of the equipment operating procedures and the need for human intervention in most cases, resulting in long processing and testing times for pressure cores. This calls for more automated equipment to reduce core handling times and make it possible to efficiently handle large numbers of cores in a short period of time.</p>
</list-item>
<list-item>
<p>Functional integration. The cost of removing natural gas hydrate reservoir cores is high, and the number of high-quality core samples is very limited. In order to obtain as much test data as possible from the limited pressure cores, it is necessary to develop multiple physical properties joint testing techniques in the field, so that the test results can be corroborated by multiple means in the limited space, limited time and limited cores.</p>
</list-item>
<list-item>
<p>Develop a multi-scale coupled permeability testing system. By integrating <italic>in-situ</italic> CT imaging with an intelligent pressure control module, combined with digital core simulation and joint inversion of experimental data, establish a dynamic correction model for the Klinkenberg effect that considers pore structure characteristics, enabling precise conversion from apparent permeability to intrinsic permeability, and providing more reliable permeability parameters for natural gas hydrate reservoir evaluation.</p>
</list-item>
<list-item>
<p>Develop a preparation and testing system for fissure-type natural gas hydrate deposits. Develop a large-scale hydrate fissure simulation device that integrates a triaxial stress loading system with real-time CT monitoring. Develop directional fracturing methods based on acoustic/microwave excitation to achieve precise control of the fissure network. Provide technical support for the industrial development of natural fissure-type hydrate reservoirs.</p>
</list-item>
<list-item>
<p>During the processing of natural gas hydrate cores, the risk of hydrate dissociation is a key factor affecting sample fidelity and experimental reliability. Although existing systems (such as PCATS and Mini-PCATS) suppress dissociation through high-pressure, low-temperature environments, local temperature increases, pressure fluctuations, and pad damage caused by mechanical cutting may still lead to hydrate decomposition at the microscopic scale. Future improvements could involve developing liquid nitrogen-assisted blades to suppress cutting-induced thermal effects, or dynamically adjusting pressure and cooling rates to precisely maintain the metastable window. Such enhancements would further enhance the accuracy of hydrate exploration data.</p>
</list-item>
<list-item>
<p>Current ship-based hydrate core testing technologies (such as CT and P-wave) prioritize screening efficiency over absolute accuracy. However, the unique phase transition characteristics of hydrates and sample heterogeneity pose challenges to traditional geotechnical testing methods (such as triaxial tests). Future research should focus on: 1) Developing calibration methods suitable for hydrate cores and establishing an error assessment system that accounts for phase transition effects; 2) Integrating machine learning technology to enhance the consistency of <italic>in-situ</italic> measurements through multi-modal data fusion (CT-P-wave-resistivity); 3) Constructing a hydrate core database to quantify the correlation between geological factors and testing variability. These efforts will drive the transformation of hydrate core testing from qualitative screening to quantitative characterization, providing a more reliable data foundation for resource assessment.</p>
</list-item>
</list>
</sec>
</body>
<back>
<sec id="s5" sec-type="author-contributions">
<title>Author contributions</title>
<p>QG: Funding acquisition, Writing &#x2013; original draft. JC: Writing &#x2013; review &amp; editing, Funding acquisition. LN: Investigation, Writing &#x2013; original draft. JS: Funding acquisition, Writing &#x2013; review &amp; editing.</p>
</sec>
<sec id="s6" sec-type="funding-information">
<title>Funding</title>
<p>The author(s) declare financial support was received for the research and/or publication of this article. This study was supported by the Science Foundation of Donghai Laboratory (No. L24QH011 and No. L24QH010), Key R&amp;D Program of &#x201c;Jianbing Lingyan+X&#x201d; in Zhejiang Province (2024SSYS0089) and the Donghai Laboratory (DH-2022ZY0007).</p>
</sec>
<sec id="s7" sec-type="COI-statement">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec id="s8" sec-type="ai-statement">
<title>Generative AI statement</title>
<p>The author(s) declare that no Generative AI was used in the creation of this manuscript.</p>
<p>Any alternative text (alt text) provided alongside figures in this article has been generated by Frontiers with the support of artificial intelligence and reasonable efforts have been made to ensure accuracy, including review by the authors wherever possible. If&#xa0;you identify any issues, please contact us.</p>
</sec>
<sec id="s9" sec-type="disclaimer">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors&#xa0;and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
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