ColBIO-TL-53, ColBIO-TL-435, ColBIO-TL-442, ColBIO-TL-450, ColBIO-TL-451, all collected at Abrolhos tidepool, 1.5 m deep, on 20/i/2011, 8/xii/2011, and 9/xii/2011.

Colonies incrusting, thin and brittle, up to 2 cm in length and 0.6 mm thick, with pale orange color. Tunic surface smooth. Spicules abundant throughout the tunic, except for the thin bladder cell layer on the surface. Spicules spherical, with short and blunt rays emerging from polygonal bases. Some spicules have one to three conical rays notably longer than the others. Central portion of the spicules without the longer rays measuring around 25 μm, and on those with long rays it ranges from 30 to 60 μm.

Zooids yellowish colored, around 1 mm in length. Thorax with almost the same size of abdomen, slightly triangular, with the branchial portion narrowing down toward the esophageal neck. Branchial siphon with a short base and 6 long, pointy lobes, measuring 3–4 times the size of the rim in some zooids. Branchial sac with 4 stigmatal rows and 7 stigmata on the first half row. Atrial opening located at the third row position and exposing just a small portion of the branchial sac. Lateral organ round and small, close to de edge of the atrial opening at the level of the third row. Thorax with strong longitudinal muscles on both sides, originating at the dorsal region and passing through the atrial opening to form the muscular process just after the thorax. Esophageal neck short and straight. Stomach globular, smooth, and yellowish colored. Midgut with 2 posterior constrictions. Posterior stomach present. Gonads within the gut loop, with a single testis and spiraled vas deferens with 5–6 counterclockwise coils. Ovary with a single developed oocyte.

Larvae gemmiparous, oval-shaped, whitish, measuring over 330 μm, with 3 papillae and 4 ectodermic ampullae. Ocelus and statocyst present. Tail coiled around 3/4 of the trunk.

Didemnum digestum has its type locality in Indonesia (Sluiter, 1909), with further records in Fiji (Kott, 1981), Moorea and Tahiti (Monniot and Monniot, 1987), and Guam (Lambert, 2003). All records are restricted to the Indo-Pacific.

The main diagnostic character for the species is spicules with one to three notably longer rays. The same type of spicule is found in Didemnum uturoa Monniot and Monniot, 1987, which in turn has two testicular follicles. Didemnum sphaericum Tokioka, 1967 also has spicules resembling those of D. digestum, especially the polygonal hollow bases of the spicule rays, but it does not have the typical longer rays (Kott, 1981). Kott (1981) described the specimens from Fiji with only 4 stigmata on the first half row, but Monniot and Monniot (1987) mentioned 7 stigmata, as observed in the specimens here described. The difference is not usual for didemnids, and the two forms might in fact be different species. The type material was not examined, but according to Kott (1981) the holotype is immature, and one of the designated types was included in the description of Didemnum multispirale Kott, 2001.

The specimens from Rocas Atoll fit into the description of D. digestum in every aspect despite the great distance from its previously known area of occurrence. In this regard, this species may well have a more widespread distribution, and be present in many tropical areas that have not been properly investigated.

ColBIO TL-49, TL-439, Abrolhos tidepool, 1.5 m, 20/i/2011; and 8/xii/2012; ColBIO TL 468, Barreta Falsa tidepool, 1.5 m, 11/xii/2011; ColBIO TL 456-461, Farol II tidepool, 1.6 m, 10/xii/2011; ColBIO TL 477, Salãozinho tidepool, 2 m, 12/xii/2011.

Colonies incrusting, thin and brittle, up to 2.5 cm in length and 0.8 mm thick. Bright orange color when alive, fading to pale orange in formalin. Colony surface rough, granulated, marked by microscopic papillae uniformly distributed. Spicules densely packed throughout the tunic, of three types: the most abundant is star-shaped, 30–50 μm in diameter, 5–8 long conic rays in the same optical plane; second type also star shaped, 25–40 μm in diameter, but frequently 8 short conical rays in the same plane; third type tetrahedral, about 50 μm and rays measuring 25 μm.

Zooids pale orange, 0.6 mm in length. Thorax triangular and slightly larger than the abdomen. Branchial sac with 4 stigmatal rows and 6-5-4-4 stigmata per half row. Branchial siphon short, wide, with 6 small pointy lobes and strong circular muscles. Atrial opening wide, exposing most of the branchial sac. Lateral organ small and round, at the fourth row position. Muscular process long, larger than the abdomen, attached to the base of the thorax by two muscle bands. Esophageal neck equivalent to 1/3–1/4 of the zooid length. Stomach globular, smooth, and yellowish. Gut with 2 midgut constrictions. Gonads included in the gut loop. Single testis, with 6 counterclockwise coils in the vas deferens. Ovary with a single developed oocyte.

Didemnum granulatum has the Tokara Islands (Japan) as type locality and was originally described as a subspecies of Didemnum moseleyi Herdman, 1886 (Tokioka, 1954). Monniot and Monniot (1994) presented the first record in the Atlantic, in the Madeleine Islands, in Senegal. One year later it was also reported in southeastern Brazil (Rocha and Monniot, 1995), and it was then detected along the country's tropical coast (Lotufo, 2002). Rocha and Kremer (2005) recorded D. granulatum on the southern coast of Brazil and considered the species as non-native in the Atlantic.

The specimens were usually found on shadowed crevices in vertical walls inside the tidepools. Indeed, the colonies are not large compared to those observed in coastal areas, which is probably due to ecological constraints on their growing pattern.

The analyzed specimens match the available descriptions (Tokioka, 1954; Monniot, 1995; Monniot and Monniot, 2001; Lotufo, 2002; Rocha et al., 2005; Lotufo and Silva, 2006), but none of the descriptions mention the presence of tetrahedral spicules, which despite being rare, were seen in all analyzed colonies.

ColBIO TL 54-56, 436, 440, and 452-454, Abrolhos tidepool, 1.5 m, 20/i/2011, 8/xii/2011 and 9/xii/2011. ColBIO TL-484, Âncoras tidepool, 1.5 m, 13/xii/2011.

Colonies incrusting, very thin (<1 mm) and brittle, measuring up to 1 cm. Tunic surface smooth, without visible cloacal openings. Color white, with spicules packed throughout the tunic, of 3 different types: the more common is spherical, 25–60 μm in diameter, with about 14 short conical rays in the same optical plane; star-shaped, 25–60 μm, with 10 long conical rays in the same plane; spherical, 12–35 μm in size and many cylindrical rays with rounded tips.

Zooids are whitish, measuring around 0.6 mm in length. Thorax smaller than the abdomen. Branchial siphon short and narrow, with 6 pointy lobes and strong circular muscles. Atrial opening generally small, depending on the contraction state of the zooid, and exposing only a small part of the second and third stigmata rows. Lateral organ as a round depression, located in a central position between the second and third stigmata row. Muscular process about the same size as the thorax, attached to the base of the thorax or sometimes farther on the esophageal neck.

Esophageal neck corresponding to 1/4 of the total zooid length. Stomach globular, smooth, and yellow colored. Abdomen flexed under the thorax, assuming a near horizontal position. Gut marked by two constrictions after the stomach, on the first half of the intestine. Posterior stomach present. Gonads included in the gut loop. Single testis, with a vas deferens with 6–7 counterclockwise coils. Ovary with a single developed oocyte, slightly larger than the testis.

Larvae unremarkable, oval and whitish, measuring around 320 μm, with 3 papillae and 4 large and round ectodermic ampullae. Oozooid in a more posterior position, Ocellus and statocyst present. Tail coiled around 3/4 of the body.

Didemnum halimedae was known solely from Caribbean waters. Described by Françoise Monniot (1983a) based on specimens from Guadeloupe, the species got its name because it was frequently found growing over green calcareous algae (Halimeda) or dead coral. Goodbody (2003) recorded the species in Jamaica, attached to old shipwrecks and not associated with Halimeda. At Rocas, it was found over the same substrates used by other species, mainly crustose coralline algae. Didemnum halimedae resembles Didemnum algadescens Monniot and Monniot, 2001 from the Pacific, which is also found over Halimeda, but differs in spicule shape.

The Rocas specimens were more variable in terms of the spicule's shapes, but the most abundant type match the holotype description. The zooids of the Brazilian specimens were slightly smaller, and the muscular process was mostly attached to the base of the thorax, but this is related to the contraction state. The lateral organ of Rocas's specimens is also larger than described by Monniot (1983a). However, there were no other distinctive characters to separate it from D. halimedae. This is the first record of the species outside of Caribbean waters.

Holotype: MZUSP 00089, 1 colony, Âncoras tidepool, 1.6 m, 13/xii/2011. Paratypes: MZUSP 00090-00094, Cemitério Island sandstone reefs, 1 m, 30/i/2011 and 15/xii/2011.

The species was named after Dr. Rosana Moreira da Rocha, in recognition of her contributions to the knowledge of the group.

Colonies white when alive, incrusting, thin (1.2 mm thick) and brittle, measuring up to 1 cm in length, generally growing on small crevices. Tunic surface smooth, devoid of bladder cells, branchial siphon positions marked by slight elevations. One single inconspicuous cloacal opening on each colony. Cloacal channels wide, at the thoracic level, right under a thin superficial tunic layer. Zooids positioned vertically. Spicules abundant in the whole tunic, of two different types: the most common measures 35–45 μm in diameter, star-shaped, with 10–13 thick rays with rounded tips per optical section; second type spherical, 25–50 μm in diameter, with 10 short conical rays on the same plane.

Zooids whitish, about 0.8 mm long, embedded vertically in the colony. Thorax long and barrel shaped, measuring about 300 μm in length. Branchial sac with 4 stigmatal rows, with 9–10 stigmata per half row. Branchial siphon short, with conspicuous circular muscles and 6 small rounded lobes. Atrial opening at the 2nd and 3rd row position, exposing part of the branchial sac. Lateral organ small and flat, at the 4th row position, close to the atrial opening. Muscular process very short and strong, attached to the base of the thorax or first half of the esophageal neck.

Esophageal neck about 1/4 of the total zooid length. Stomach rounded, smooth, and yellow. Midgut with one constriction right after the stomach, and a second one at the distal portion of the abdomen. Gonads included in the gut loop, with a single large and spherical testis and a slightly smaller oocyte. A small mass of cells is usually observed over the testis, close to the ovary. Vas deferens spiraled with 6–7 counterclockwise coils.

Larvae oval and whitish, with 360–375 μm in length, with 3 papillae and 4 pairs of large and rounded ectodermal ampullae, incubated in the basal portion of the colony. Ocellus and statocyst present in the mid portion of the trunk. Tail coiled around 3/4 of the larvae.

Overall, the analyzed specimens resemble the description of Didemnum speciosum (Herdman, 1886) in terms of general colony aspect and structure (Rocha and Monniot, 1995; Lotufo, 2002) but differ in the spicules' size and shape. (Millar's, (1977) description of D. speciosum may well match the present species, but the author was certainly mixing different species into one description, as later corrected by Rocha and Monniot (1995). Didemnum ahu Monniot and Monniot, 1987 is also similar to the present species in many aspects, especially zooid morphology. However, specimens from New Caledonia and French Polynesia have spicules of similar size but with needle-stack rays (Monniot and Monniot, 1987; Monniot, 1995). Specimens from Palau, in particular, have spicules measuring up to 500 μm, with short and thick rays (Monniot and Monniot, 2008). Also, in descriptions from both Palau and French Polynesia, the cloacal channels are seen on the colony surface, a character not present in D. rochai. Further, Brazilian D. ahu specimens are yellowish or cream colored, with spicules restricted to the superficial tunic layer (Rocha and Monniot, 1995; Lotufo, 2002).

The species Didemnum algasedens Monniot and Monniot, 2001 from the West Pacific is also similar to D. rochai in terms of the colony structure, spicules, and a few aspects of the zooid morphology, such as gonads, but it has long pointy lobes around the branchial siphon, a long muscular process, a long esophageal neck, and pigmented larvae.

Although most features are commonly found in other white Didemnum species, the combination of characters does not allow the identification as any known species.

Holotype: MZUSP 00104, 1 colony, Salãozinho tidepool, 21/i/2011, 3 m.

The name refers to the bright orange color of the colony.

Colonies bright orange colored, incrusting, moderatelly thick (1.6 mm), up to 1.2 cm in length. Tunic surface smooth, with a bladder cell layer. Colony consistency soft, with zooids not arranged in any particular pattern. Cloacal openings small, three on the type specimen. Cloacal channels narrow, in thoracic position, sometimes reaching spaces under the zooids. Spicules small and spherical, concentrated on the superficial layer of the tunic, sparsely distributed on the interior of the colony, measuring around 12 μm, with numerous irregular, thin and short conic rays. Spicules form also a thin layer on the underside of the colony.

Zooids yellowish, up to 1.0 mm in length. Branchial sac with 10-9-9-8 stigmata per half row. Branchial siphon small, narrow, and devoid of conspicuous lobes. Atrial opening small and tubular, with smooth rim, located at the 2nd and 3rd stigmata row level and directed dorsally. Lateral organ very small, located at the base of the atrial siphon. Thorax with strong longitudinal muscle bands. Muscular process absent.

Esophageal neck short. Stomach globular, smooth, and yellow. Midgut with two constrictions on the proximal third. Posterior stomach present. Gonads included in the gut loop, with 3 testicular follicles and a spiraled vas deferens with 2–3 counterclockwise coils. Oocytes or larvae were not present.

The genus has few species in the Atlantic: Leptoclinides brasiliensis Michaelsen, 1923, Leptoclinides faeroensis Bjerkan, 1905, Leptoclinides latus Monniot, 1983, and Leptoclinides torosus Monniot, 1983 (Rocha et al., 2012). Among them, L. latus is the most similar to the present species, originally described from specimens from Guadeloupe, with a single record for southern Brazil (Rocha et al., 2005). However, L. latus zooids usually have a bifid atrial languet as well as a pyloric gland around the gut on the posterior side of the stomach (Monniot, 1983a), characters not seen in Leptoclinides crocotulus.

Leptoclinides robiginis Monniot, 1989, with the type locality of New Caledonia, also presents spicules concentrated on the superficial layer of the colony and a vas deferens with 2–3 coils, but differs in spicule size, color pattern, branchial siphon lobes, and a bifid atrial languet, as in L. latus (Monniot, 1983a, 1989).

Therefore, Leptoclinides crocotulus does not fit into any available description.

Holotype: MZUSP 00095, Âncoras tidepool, 1.6 m, 13/xii/2011; Paratypes: MZUSP 00099-00101, Barreta Falsa tidepool, 1.6 m, 11/xii/2011; MZUSP 00103, Salãozinho tidepool, 3 m, 21/i/2011.

The species is named after Maurizélia de Brito Silva due to her tireless work managing and protecting the Rocas Biological Reserve.

Colonies thin (~1.4 mm thick), small, and incrusting, up to 1.0 cm in length. When alive, tunic orange colored on the surface and bright red inside. Tunic surface smooth when the colony is turgid, becoming wrinkled when removed from substrate. Two or three cloacal openings in each colony. Common cloacal cavity short and restricted to thoracic level, but occasionally also at abdominal level. Spicules densely packed on the superficial layer, becoming rare inside the colony. Spicules small, 10–20 μm, of two types: most common type spherical, with over 10 small conic rays on the same plane (Figure 7C); second type star-shaped, with 7 long conic rays per optical section (Figure 7B).

Zooids yellowish, measuring around 1.5 mm. Thorax and abdomen of equal sizes. Branchial sac with 4 stigmatal rows and 12 stigmata on the first half row. Branchial siphon wide, about 1/3 the size of the branchial sac, with 6 small sharp lobes and conspicuous circular musculature. Atrial opening wide, at the middle of the thorax. Atrial languet single, flap-like, absent in most zooids. Lateral organ very small and circular, positioned at the third row level. Thorax with about 5 longitudinal muscle bands, equally spaced. Muscular process absent.

Esophageal neck short, less than 1/3 the total zooid length. Stomach globular, smooth-walled, yellow. Midgut with constrictions defining a posterior stomach. Gonads inside the gut loop, with 3–4 testicular follicles and a vas deferens with 3 counterclockwise coils. Ovary with a single developed oocyte.

Larvae oval-shaped, with few granular cells present on the external epithelium, yellowish, 860 μm in length, with 3 papillae and 27 rounded ectodermal ampullae disposed as a crown. Statocyst and ocellus present at the half of the trunk. Tail coiled around 1/2 the trunk.

The present species bears no resemblance with any other Polysyncraton recorded from Brazilian waters. The most common species of this genus in Brazil is Polysyncraton aff. amethysteum (Lotufo, 2002; Dias et al., 2012), which has a different color pattern, spicules, and larvae. However, other Atlantic species seem more related to the one described herein, such as Polysynrcaton bilobatum Lafargue, 1968 regarding the color and spicule shape, and Polysyncraton reedi Monniot and Monniot, 1994, which is similar in terms of the testis structure, but has a wider variation interval in the number of follicles (3–6) and coils in the vas deferens (2–3).

The most noticeable character of the colony is its wrinkled aspect after removal from the substrate, a feature also present in Polysyncraton rugosum Monniot, 1993, which also has a similar color and does not have a muscular process. The differences are in the distribution of spicules in the tunic and the structure of the cloacal openings, with dark edges devoid of spicules. In addition, the size of the larvae (1.1 mm) is also a character that clearly allows the distinction (Monniot, 1993).

Although the wrinkled aspect of the colony is also present in other species, such as Polysyncraton asterix Monniot, 1974 and Polysyncraton pseudorugosum Monniot, 1993, there are important differences related to the size and shape of the spicules and the presence of a muscular process (Monniot, 1974a, 1993).

ColBIO TL 52, 437, 438, 447-449, Abrolhos tidepool, 1.6 m, 20/i/2011, 8/xii/2011, and 9/xii/2011; ColBIo TL 514, Cemitério tidepool, 1 m, 11/xi/2011; ColBIO TL 47-48, Donzelinha tidepool, 1 m, 18/i/2011; ColBIO TL 58-60, 473-476, Salãozinho tidepool, 4 m, 21/i/2011 and 12/xii/2011.

Colonies easily removed from substrate, incrusting, up to 2.5 cm in length and 5 mm thick. Color varying from green-brown and reddish-brown on shaded areas to gray-white on light exposed spots. Colonies have rounded edges and grow clustered together, giving them a jigsaw aspect. Five to seven cloacal openings on each colony. Tunic firm and rigid, somewhat brittle, with spicules packed in the whole extension, except for a thin superficial layer. Spicules large, sizes varying from 30 to 75 μm, but most are 50 μm, with around 9 short conical rays on the same optical plane.

Zooids white, 1.0 mm long. Thorax larger than abdomen. Branchial sac with 9–11 stigmata on the first two rows and 6–8 in the third and last row. Branchial siphon long, about the size of the branchial sac, with 6 long and sharp lobes, frequently pigmented with a dark squamous epithelium. Atrial opening small and tubular, about 1/2–1/3 the size of the branchial siphon, inserted at the second stigmata row level. Few zooids may present a wider atrial opening. Thorax with 7 longitudinal muscle bands, and siphons with marked circular muscles and thin longitudinal muscles. Lateral organ large and circular, positioned at the second or third row level. Muscular process short, inserted at the base of the thorax.

Esophageal neck about 1/3 the total zooid length. Stomach small, globular, smooth and yellowish. Midgut with 2 constrictions. Gonads inside the gut loop. Single testis large, with 7–8 counterclockwise coils in the vas deferens. A single oocyte developed on each mature zooid.

Larvae large, measuring 1.0 mm, rounded, with 3 papillae and 11 pairs of ampullae.

There are few Trididemnum species recorded from the Brazilian shores: Trididemnum maragogi, Trididemnum orbiculatum (Van Name, 1902), and Trididemnum solidum Van Name, 1902; (Van Name, 1945; Rocha et al., 2012).

Trididemnum maragogi was described based on specimens from Alagoas (Rocha, 2002), and this is the only record for Brazil. The species was also registered in Panama (Rocha et al., 2005). It is strikingly similar to T. solidum, but the distinctive character pointed out by Rocha (2002) regarding the size of the branchial siphon, number of stigmata per row, and vas deferens coils were also noticed in the animals from Rocas. However, it seems reasonable to investigate the validity of this species with an analysis of a greater number of colonies from different locations, along with a genetic study to determine whether the differences between these two species simply reflect intraspecific variation.

Holotype: MZUSP 00105, Cemitério Island sandstone reefs, 1 m, 10/xii/2011. Paratypes: MZUSP 00106, Abrolhos tidepool, 1.6 m, 9/xii/2011; MZUSP 00115, Barretinha tidepool, 1.5 m, 23/i/2011; MZUSP 00107-00112, Farol II tidepool, 1 m, 10/xii/2011; MZUSP 00113-00114, Cemitério Island sandstone reefs, 1 m, 15/xii/2011.

The species received the epithet from its type locality.

Colonies small and incrusting, up to 2.7 cm in length and 1.5 mm thick. Color black or dark gray, with white spots marking the spicules around the cloacal openings. Tunic fragile and soft, with spherical or elongated brown pigmented cells, mainly on its surface and lining the common cloacal cavity. Cloacal openings large, 1–3 per colony. Common cloacal cavity at the thoracic level, sometimes extending into a posterior abdominal cavity. Spicules absent on the tunic superficial layer, except around the cloacal openings, and abundant on the middle layers, mainly lining the common cloacal cavity, becoming scarce at the basal portion. Spicules of three different types: the most common larger and spherical, 75 μm and short conical rays; another 50 μm and cylindrical rays; last type smaller, with 25 μm and long, thin rays.

Zooids measuring around 1.0 mm, covered with squamous epithelial cells giving it a green-brown color. Thorax slightly smaller than the abdomen. Branchial sac with three stigmata rows. Branchial siphon short, large, and pigmented, generally with 6 lobes, usually rounded, but a few zooids have more pointed ones. Atrial opening tubular, small, devoid of squamous cells, and slightly longer than the branchial siphon, with a few zooids presenting a wider opening. Muscular process strong, about the size of the thorax, formed by 7 converging muscle bands.

Esophageal neck short and wide, typically with a muscular ring in the transition to the abdomen. Stomach rounded, smooth and yellowish. Midgut with two constrictions and posterior stomach present. Gonads with a single testis and 6–7 counterclockwise coils in the vas deferens; a single well-developed oocyte, sometimes with a second smaller oocyte in development.

Larvae oval-shaped, white, 700 μm long, with 3 long stalked papillae and 3 pairs of drop shaped ectodermal ampullae. Statocyst and ocellus present at the limit of the posterior half of the trunk. Tail coiled around 3/4 of the larval trunk.

Trididemnum rocasensis sp. nov. is the most widespread and abundant species in Rocas' shallow waters, where it is usually found in exposed spots on the reef as well as in small crevices inside tidepools. The species resembles Trididemnum banneri Eldredge, 1966 in colony color, spicule size, and overall larvae morphology (Eldredge, 1966). However, T. banneri larvae may present 3 or 4 pairs of ampullae, and the lobed atrial opening and larger number of coils in the vas deferens also distinguish this species from T. rocasensis (Monniot and Monniot, 1987; Monniot, 1990/1991).

Trididemnum areolatum Herdman, 1906, Trididemnum cerebriforme Hartmeyer, 1913, and Trididemnum hians Monniot, 1983, among many others, also have dark pigmented cells in the tunic, but differ in the spicule size or shape, endostyle pigmentation, number of coils in the vas deferens, and larvae morphology (Kott, 1981, 2001; Monniot, 1983a).

Trididemnum hians is also similar to T. rocasensis sp. nov. in colony pigmentation and zooid structure, but it has a larger number of ampullae in the larvae (5 pairs) and different spicules.

ColBIO TL 487, Barreta Falsa tidepool, 1.5 m, 14/ xii/2011.

Individual small, about 3.8 mm long, with a thin transparent tunic. Body wall also transparent, with few longitudinal muscle fibers on the right side, running from the branchial siphon. Transversal muscle fibers starting at the atrial siphon and reaching half of the body. Muscles on the left side almost absent, with few short fibers disposed perpendicularly to the dorsal margin, but present only by the gut. Circular muscle fibers visible in both siphons.

Branchial siphon short, with 8 lobes. Atrial siphon also short, with 6 lobes, positioned at the posterior region. Prepharyngeal groove simple, without papillae in the whole prepharyngeal area. Oral tentacles simple, long, and thin, 21 in total, of 3 orders. Dorsal tubercle small and rounded, 0.3 mm from the tentacles ring, and 1.2 mm from the neural gland. Dorsal lamina smooth-edged. Branchial sac with 18 longitudinal vessels on the right side, 15 on the left, and 27 transverse vessels and 2 stigmata per mesh. Primary papillae simple, but intermediary papillae and parastigmatic vessels absent. Numerous renal vesicles over the stomach and ascending portion of the gut. Hindgut dilated. Immature specimen.

The present species resembles Ascidia molguloides Monniot, 1974 in regard to the minute size and body muscles as well as the number of oral tentacles (25). The number of longitudinal vessels on the branchial sac, on the other hand, if far greater in A. molguloides (27–30 on the left side, 29–33 on the right side). There are also differences in the gut loop, which is wider in the North Atlantic species, as well as the tunic completely covered by sand (Monniot, 1974b). All these differences point to a new species, but as the single specimen was immature, we opted not to name it.

ColBIO TL 434, Abrolhos tidepool, 1.6 m, 8/xii/2011; ColBIO TL 43, 68, Cemitério Island sandstone reefs, 1 m, 16/i/2011 and 30/i/2011.

Colonies small, up to 3 cm long, with few zooids, bright red color when alive. Zooids clustered irregularly, more or less individualized, but covered by a common tunic. Tunic firm, rough, and resistant.

Zooids 1 cm long when removed from tunic, dorsoventrally flattened. Body wall yellowish, translucid, with endocarps present. Both siphons smooth-edged and of equal sizes. Atrial siphon with pronounced longitudinal muscles extending to half the body length. Branchial siphon vellum with grooves, giving it a wrinkled aspect. Branchial sac with 4-folds on each side. Oral tentacles simple, filiform, around 30 in total, of 3 different size orders. Dorsal tubercle oval-shaped. Dorsal lamina smooth-edged. Branchial sac with over 62 longitudinal vessels, about 30 on the left side and 32 on the right side. Approximately 20 transverse vessels, and 10–12 stigmata per mesh. Stomach large, orange, barrel-shaped, with about 13-folds and a thumb-shaped cecum curved toward the intestine. Gonads at the ventral region, but strongly attached to the tunic. Gonads composed of 2 oval testicular lobes, surrounded by numerous round oocytes.

This is an abundant species in Rocas and is found as individual zooids or small clusters on the underside of loose stones around the reef at Cemitério Island.

The species is widely distributed along the Atlantic, occurring from Florida to southern Brazil (Rocha et al., 2012). The records are concentrated in the Caribbean region, where it seems to be very common. It was found in Belize (Goodbody, 2000), Bermuda (Berril, 1932; Monniot, 1972), Cuba (Hernández-Zanuy and Carballo, 2001), Curaçao (Goodbody, 1984), Guadeloupe (Monniot, 1983c; Monniot and Monniot, 1985), Jamaica (Goodbody, 2003), and Panama (Rocha et al., 2012). The species was also recorded in Senegal, West Africa (Pérès, 1951) and Inhaca Island, Mozambique, in the Indian Ocean (Millar, 1961).