Front. Insect Sci. Frontiers in Insect Science Front. Insect Sci. 2673-8600 Frontiers Media S.A. 10.3389/finsc.2022.1110480 Insect Science Original Research The impact of host plant species on instar duration and body weight of nymphal Lycorma delicatula Kreitman Devin 1 Keena Melody A. 2 Nielsen Anne L. 1 Hamilton George 1 * 1 Entomology Department, Rutgers, The State University of New Jersey, Brunswick, NJ, United States 2 Northern Research Station, USDA Forest Service, Hamden, CT, United States

Edited by: Xiaoyi Wang, Chinese Academy of Forestry, China

Reviewed by: Arthur Vieira Ribeiro, University of Minnesota Twin Cities, United States; Chuanwang Cao, Northeast Forestry University, China

*Correspondence: George Hamilton, ghamilto@njaes.rutgers.edu

This article was submitted to Invasive Insect Species, a section of the journal Frontiers in Insect Science

 19 01 2023 2022 2 1110480 28 11 2022 28 12 2022 Copyright © 2023 Kreitman, Keena, Nielsen and Hamilton 2023 Kreitman, Keena, Nielsen and Hamilton

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

The spotted lanternfly, Lycorma delicatula (White) (Hemiptera: Fulgoridae), is an invasive species of planthopper that was introduced to North America and is a threat to multiple industries. Nymphs and egg masses were collected to assess each instar’s rate of development at a constant temperature of 25°C on the following hosts: Ailanthus altissima (Miller) (Sapindales: Simaroubaceae), Vitis labrusca (L.) (Vitales: Vitaceae), Salix babylonica (L.) (Malpighiales: Salicaceae), Acer rubrum (L.) (Sapindales: Sapindaceae), Celastrus orbiculata (Thunberg) (Celastrales: Celastraceae), Ocimum basilicum (L.) (Lamiales: Lamiaceae), and Rosa multiflora (Thunberg) (Rosales: Rosaceae). Host plant species was found to have a significant effect on developmental time for nymphs in the first through third instars, as well as on nymphal survival. Nymphs failed to develop through the second instar on O. basilicum and the third and fourth instars on A. rubrum. Host plant species also had a significant effect on the mean weight of nymphs in the first, second, and fourth instars (but not in the third instar), and on the hind tibia length and forewing width of adult nymphs. This variability in L. delicatula developmental time by host plant species can potentially impact phenology models, which should be updated to reflect these new insights. Rearing practices should also be refined to account for host plant influences on the physiology of L. delicatula.

 phenology survival development hostplant lanternfly Animal and Plant Health Inspection Service10.13039/100009168 Animal and Plant Health Inspection Service10.13039/100009168 Introduction

The spotted lanternfly, Lycorma delicatula (White) (Hemiptera: Fulgoridae), is an invasive species of planthopper that was first detected in the United States in the summer of 2014. L. delicatula is native to China, Vietnam, and India; the United States is one of three countries invaded by this species, together with Japan and South Korea (1). Since its initial detection in Pennsylvania, L. delicatula has spread across the northeastern region of the United States and is now established in multiple states (1).

L. delicatula has four instars. The first-instar nymphs start to emerge from eggs in late April in North America (2). Nymphs of the first three instars are black and white, and fourth-instar nymphs are black, white, and red in color. Adults appear around mid-July and lay eggs from early September until temperatures are low enough to kill them. The eggs are deposited in grayish to tan-colored egg masses on various substrates, such as bark, stone, wood fences, and brick, on which the egg masses overwinter until the following spring.

L. delicatula has a broad host range consisting of 103 plant species (2). Despite this, L. delicatula has a preferred host, which is the tree of heaven, Ailanthus altissima (Miller) (Sapindales: Simarobaceae) (3). Recently, it was found that L. delicatula does not require A. altissima to complete its lifecycle, but that the removal of A. altissima from its diet is associated with reduced fitness (4). However, despite being widespread and commonly found in disturbed sites, A. altissima is not always available as a host for L. delicatula.

External temperature has a major influence on the development and growth of insects; however, other factors can also influence their growth. Previous research has shown that the host plant can affect an insect’s phenology and should be considered in phenology models (5, 6). For example, the larvae of the Oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), develop more quickly when feeding on Prunus persica (L.) (Rosales: Rosaceae) than when feeding on Malus domestica (Borkhausen) (Rosales: Rosaceae) (7). Likewise, nymphs of the brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), from the second instar onward were found to develop more quickly when reared on P. persica than when reared on M. domestica (8).

The phenology of L. delicatula has been previously determined on A. altissima and Parthenocissus quinquefolia (L.) (Vitales: Vitaceae) (9, 10). In the latter study, when L. delicatula was reared on P. quinquefolia at room temperature (assumed to be slightly above 20°C), it was found that the developmental duration of the first-, second-, third-, and fourth-instar nymphs was 18.8, 20.9, 20.8, and 22.2 days, respectively (10). When L. delicatula was reared on A. altissima at that temperature, it was found that the duration of the first-, second-, and third-instar nymphs was 23.4, 24.0, and 40.4 days, respectively (9). The data for the fourth-instar nymphs were separated by sex, with male and female nymphs completing that instar within 39.5 and 50.1 days, respectively. These differences in results, with L. delicatula taking less time to develop on P. quinquefolia than on A. altissima at 20°C, suggest that host plant species also influences their development. In addition, fourth-instar nymphs were found to take fewer days to develop at 25°C when they were reared on fox grape, Vitis labrusca (L.) (Vitales: Vitaceae), than on A. altissima in an unpublished study (8), a finding which further stresses the importance of determining the developmental rate of L. delicatula on different host plants.

To further understand the effect of host plant species on the development of L. delicatula, it is important to rear nymphs on a variety of different host plants. In this study, the survival and development of nymphs and the weight and size of L. delicatula adult insects were examined using one of the following plants as a host: tree of heaven (A. altissima), fox grape (V. labrusca), weeping willow [Salix babylonica (L.) (Malpighiales: Salicaceae)], red maple [Acer rubrum (L.) (Sapindales: Sapindaceae)], Oriental bittersweet [Celastrus orbiculata (Thunberg) (Celastrales: Celastraceae)], basil [Ocimum basilicum (L.) (Lamiales: Lamiaceae)], and multiflora rose [Rosa multiflora (Thunberg) (Rosales: Rosaceae)]. The results from this study will help to further advance phenology models for this insect.

 Methods Source populations

On 17 June 2020, L. delicatula first-instar (n = 140) and second-instar (n = 63) nymphs were collected at a site in Hunterdon County, New Jersey, USA (Riegelsville, NJ). The site had Vitis spp., Rosa spp., C. orbiculata, A. altissima, Celtis occidentalis (L.) (Rosales: Cannabaceae), and Juglans nigra (Fagales: Juglandaceae), as well as other assorted unidentified shrubbery. The nymphs were found mostly in the shade, and egg masses were observed on site. The nymphs were transferred to a quarantine facility located in Ansonia, Connecticut, USA, as per the terms of the US Department of Agriculture Animal and Plant Health Inspection Service (USDA APHIS) permits, in containers containing a single 50- to 70-cm-long sprig of wild grape, Vitis volpina L. (Vitales: Vitaceae), to sustain them for the trip. At the quarantine facility, the nymphs were sorted by instar and placed into a large mesh cage (60 cm × 60 cm × 120 cm; BugDorm 6S620, MegaView Science Co., Ltd, Taichung, Taiwan) with two or three 100-cm-tall potted A. altissima plants and a single V. labrusca plant and kept at 25°C with a photoperiod of 16 h : 8 h (L : D) and a relative humidity between 60% and 80%. Once the nymphs began molting to the next instar, 10 that molted on the same day were taken and set up in a smaller 32.5 cm × 32.5 cm × 77.0 cm mesh cage (BugDorm 4S3074, MegaView Science Co., Ltd, Taichung, Taiwan) containing two host plants of the same species for use in experiments. These smaller cages were kept at the same photoperiod, temperature, and humidity as the other larger cages. Any additional nymphs that molted were transferred to the large BugDorm cages and allowed to develop into later instars.

 Plant rearing

The host plants that were used were selected for a variety of reasons. Ailanthus altissima was selected because it is the preferred host for L. delicatula, making it a good reference for comparison with other host plants. As L. delicatula poses a significant threat to wine grapes, it is important to determine if being reared on V. labrusca influences its developmental rate. Salix babylonica was selected because it is a common landscape tree and was one of the trees used in the study that showed that L. delicatula could complete development without A. altissima (4). Celastrus orbiculata was selected based on previous literature findings indicating that L. delicatula, in the early instars, commonly used it as a host. Ocimum basilicum is a common garden plant and R. multiflora is a common forest plant, and it has been found that L. delicatula feeds on both plants.

A. altissima was grown from seeds collected in Wallingford, Connecticut, USA, in October 2019. Seeds were initially planted in Jiffy Plugs and then transferred to tree pots measuring 7.6 cm × 7.6 cm × 20.3 cm (CN-SS-TP-308, Greenhouse Megastore, Danville, IL, USA) filled with soil (Premier BK25, Promix M, Premier Horticultural Inc., Quakertown, PA, USA) after sprouting. The A. altissima seedlings were provided with 5–10 g (the amount was dependent on the size of the pot) of Osmocote fertilizer (ICL Specialty Fertilizers, Summerville, SC, USA) when they were first put into the tree pots, and monthly thereafter. Otherwise, the A. altissima seedlings were reared as described in Kreitman et al. (9).

Celastrus orbiculate was grown from cuttings from multiple plants obtained from the towns of Wallingford and Ansonia (CT, USA). Rosa multiflora was grown from cuttings obtained from multiple plants from Wallingford, Connecticut, USA, and from at least 10 individual plants from Ansonia, Connecticut, USA. Both Celastrus orbiculate and Rosa multiflora were obtained during the summer of 2020. Ocimum basilicum plants were grown from “hybrid herb, basil Prospera organic” seeds purchased from Seedway, LLC (Hall, New York, NY, USA) using the same method as for the A. altissima plants. Celastrus orbiculate, R. multiflora, and O. basilicum were all grown in the same soil and tree pots as the A. altissima plants.

The A. rubrum and S. babylonica plants were purchased from Cold Stream Farm LLC (Freesoil, MI, USA) in late March 2020. The V. labrusca bare-root plants were purchased from Double A Vineyard (Fredonia, NY, USA) and were shipped in the spring of 2020.

 The effect of different host plant species on the development of nymphal <italic>Lycorma delicatula</italic> Nymphal rearing in 2020

For this first year, the host plants used were A. altissima, V. labrusca, S. babylonica, and A. rubrum. Three cages of each host plant treatment were set up, with 10 second-instar nymphs or 10 third-instar nymphs per cage, both sourced from the rearing cages containing the field-collected nymphs. For the fourth-instar nymphs, five nymphs that molted on the same day were placed in a small cage for each host with 10 replicates of each over a period of 12 days. Each cage started with two plants, and new plants of the same host were added to the cages every 7 days for the second- through third-instar nymphs, and every 4 days for the fourth-instar nymphs. Nymphs were monitored daily for survival and molting, which was confirmed by a cast skin. Any newly molted nymphs were removed from the cages, weighed, and then preserved by freezing for later sexing. The second-instar nymphs were preserved in ethanol, and, therefore, we were unable to determine their sex. Measurements of forewing length, forewing width, and hind tibia length were taken for all frozen adult nymphs using a dissecting microscope.

 Nymphal rearing in 2021

In 2021, we evaluated only the first- and second-instar nymphs of the hosts. The nymphs were hatched (first instars) or reared (second instars) from field-collected egg masses. The egg masses were collected by removing both the egg mass and the bark substrate that it was on using a chisel and hammer, from two sites in Pennsylvania and one site in New Jersey, on 20 October 2020 ( Table 1 ). These egg masses were held individually in 60 mm × 15 mm Petri dishes (Falcon 351007, Becton Dickinson Labware, Franklin Lakes, NJ, USA) at 15°C until they hatched. On hatching, 20 nymphs that hatched from egg masses on the same day, from the same collection site, were placed in a small BugDorm cage (32.5 cm × 32.5 cm × 77.0 cm) containing two plants of the same species. The species of plants used were A. altissima, V. labrusca, S. babylonica, A. rubrum, C. orbiculata, O. basilicum, and R. multiflora. The range of host plant species was expanded in 2021 because of the promising preliminary results in 2020. Additional hatch from those egg masses was placed in the larger BugDorm cages (60 cm × 60 cm × 120 cm) with two or three 100-cm-tall-potted A. altissima plants and a single V. labrusca plant, and kept at a temperature of 25°C for rearing to be used as second-instar nymphs. For both the first- and second-instar nymphs, two cages were set up with nymphs from the New Jersey site, and one cage was set up with nymphs from each Pennsylvania site, for a total of four cages for each host. Voucher specimens were preserved in a freezer for reference, in addition to the voucher specimens of adult L. delicatula that were deposited at the Entomology Division, Yale Peabody Museum of Natural History, New Haven, Connecticut, USA.

Approximate locations (latitude and longitude), collection date, and hosts from which the egg masses of Lycorma delicatula used in this study were obtained.

Collection location Collection date Host (number of egg masses) Latitude Longitude
Spruce Run Reservoir, Clinton, NJ, USA 10 October 2020 Betula pendula Roth (93) and dead trees (23) 40°39′47.03″N 74°55′36.02″W
The Woodlands, Philadelphia, PA, USA 10 October 2020 Prunus spp. (110), Broussonentia papyrifera (L. Vent.) (Rosales: Moraceae) (8), Acer platanoides (L.) (Sapindales: Sapindaceae) (7), and Crataegus spp. (Rosales: Rosaceae) (10) 39°5′45.86″N 75°12′19.37″W
Neshaminy State Park, Bensalem, PA, USA 10 October 2020 Betula nigra (L.) (Fagales: Betulaceae) (33), Betula lenta (L.) (Fagales: Betulaceae) (18), Acer rubrum (25), Prunus spp. (18), and Pinus strobus (L.) (Pinales: Pinaceae) (24) 40°4′31.87″N 75°55′0.59″W
Statistical analyses

Statistical analyses were performed using SAS 9.4 (11). Data did not fit assumptions of normality per the Shapiro–Wilk and Anderson–Darling tests. PROC UNIVARIATE was then used to assess the fit of the data to a gamma distribution. Each model was fitted to a gamma distribution with a log-link function because the response variables had long right tails. PROC GLIMMIX was used to evaluate the fixed effect of host on the duration and body mass of each instar. If the sex was known, the fixed effects of sex and the interaction of sex and host plant species were added to the model. The state(s) in which the egg masses were collected, and of the cages, were treated as random effects. PROC GLIMMIX with a beta distribution and logit link function was used to evaluate the effects of host on the overall survival for each instar. Percentage survival was calculated for each cage. Values of 1 were replaced with 0.9999, and values of 0 were replaced with 0.0001, because the beta curve allows only values between 1 and 0. Differences among means were determined using Tukey–Kramer post hoc analysis and a α value equal to 0.05. Statistical comparisons of nymphal survival curves between host plants for each instar were carried out using a Peto–Wilcoxon test in Statistix 10.0 (12). For this analysis, any nymphs that molted or were inadvertently killed were censored.

 Results Survival

Overall, host plant species had an impact on nymphal survival. There was no significant difference in the survival curves (χ2 = 4.98, d.f. = 3, p = 0.1730), but there was a significant difference in overall survival (F3 ,9 = 5.03; p = 0.0256), between host plant species for the second-instar nymphs in 2020. In 2020, the overall percentage survival of second-instar nymphs was lowest on A. rubrum among all host plant species ( Figure 1 ). The survival curves (χ2 = 114.44, d.f. = 3, p < 0.0001 for third-instar nymphs and χ2 = 100.48, d.f. = 3, p < 0.0001 for fourth-instar nymphs) and overall survival percentages (F 3,16 = 5.12; p = 0.0113 for third instar nymphs, and F 3,36 = 9.48; p < 0.0001 for fourth-instar nymphs) were significantly different between host plant species for both third- and fourth-instar nymphs in 2020. Third- and fourth-instar nymphs reared on A. rubrum and S. babylonica had the numerically lowest percentage survival. There was a significant difference in survival between host plant species for both the first- and second-instar nymphs in 2021 (χ2 = 87.12, d.f. = 6, p < 0.0001, for first-instar nymphs and χ2 = 55.00, d.f. = 6, p < 0.0001, for second-instar nymphs). There was no significant difference in overall survival by host for first-instar nymphs (F 6,21 = 1.66; p = 0.1811), but there was a significant difference for second-instar nymphs (F 6,21 = 3.44; p = 0.0159). In 2021, overall survival was numerically highest for first-instar nymphs reared on C. orbiculate, A. altissima, and A. rubrum, and lowest for those reared on O. basilicum and S. babylonica, but there were substantial differences between the individual cages in percentage survival ( Figure 2 ). Second-instar nymphs had the highest overall percentage survival when reared on C. orbiculate, A. altissima, S. babylonica, and V. labrusca, and the lowest overall percentage survival when reared on O. basilicum.

Survivorship curves for Lycorma delicatula nymphs reared in 2020 by instar and host plant species. Different letters to the right of the graphs indicate differences between the overall survival for the host plant species.

Survivorship curves for Lycorma delicatula nymphs reared in 2021 by instar and host plant species. Different letters to the right of the graphs indicate differences between the overall survival for the host plant species.

 Nymphal development 2020 development

Host plant species did not have a significant effect on the mean time spent in the second instar in 2020 (F 3,78 = 2.49; p = 0.0660) ( Figure 3 ). Second-instar nymphs reared on A. rubrum spent significantly more time in that instar than nymphs reared on V. labrusca. In addition, host plant species did not have a significant effect on the weight of nymphs on completing the second instar (F 3,78 = 0.91; p = 0.4400).

Mean time (days) spent in instar and weight (mg) of Lycorma delicatula nymphs reared in 2020 by host plant species, instar, and sex. Means with a different letter are significantly different from each other at a p-value < 0.05 using Tukey–Kramer grouping.

For third-instar nymphs, the mean development time was significantly affected by sex (F 1,186 = 30.87; p > 0.0001), with female nymphs having longer development time ( Figure 3 ). Host plant species had a significant effect on the mean development time in the third instar (F 2,186 = 8.98; p = 0.0003); however, there was no significant difference for nymphs reared on A. altissima and V. labrusca. There was no significant interaction of host plant species and sex for third-instar nymphs (F 2,186 = 0.35; p = 0.7029). Female nymphs reared on S. babylonica spent significantly longer in the third instar than all other nymphs. None of the nymphs reared on A. rubrum were able to complete the third instar.

Sex had a significant effect on the mean weight of nymphs that completed the third instar (F 1,186 = 126.8; p < 0.0001) ( Figure 3 ). For each host plant species, female nymphs weighed significantly more than male nymphs reared on the same host plant species. Neither host plant species (F 2,186 = 1.68; p = 0.193) nor the interaction of host plant species and sex (F2,186 = 1.99; p = 0.143) had a significant effect on the mean weight of nymphs that completed the third instar. No significant difference was found in the mean weight of male or female nymphs reared on any of these hosts. In addition, female nymphs that were reared on A. altissima weighed significantly more than male nymphs that were reared on either S. babylonica or V. labrusca. Female nymphs reared on V. labrusca also weighed significantly more than male nymphs that were reared on S. babylonica.

Sex had a significant effect on the mean development time spent in the fourth instar (F 1,59 = 7.26; p = 0.009) ( Figure 3 ); however, host plant species did not have a significant effect on the mean time spent in the fourth instar (F 2,59 = 0.88; p = 0.4195). Likewise, the interaction of host and sex also did not have a significant effect on the mean time spent in the fourth instar (F 2,59 = 0.6; p = 0.5526). Female nymphs reared on A. altissima had a significantly longer developmental time than male nymphs that were reared on the same host plant. None of the fourth-instar nymphs reared on A. rubrum were able to complete the fourth instar.

 2021 development

For first-instar nymphs, host plant species had a significant effect on developmental time (F 6,262 = 24.21; p < 0.0001) ( Figure 4 ), with nymphs reared on A. rubrum having a significantly longer developmental time than those reared on all other host plant species, except for O. basilicum. There was no significant difference in mean nymphal development time in first instar between those reared on A. altissima, V. labrusca, and C. orbiculata. First-instar nymphs also spent significantly less time in the first instar when reared on A. altissima than those reared on R. multiflora, S. babylonica, A. rubrum, and O. basilicum. The weights of first-instar nymphs were also significantly affected by the host plant species (F 6,271 = 22.41; p > 0.0001), although no significant differences were observed in the weights of first-instar nymphs reared on A. altissima, V. labrusca, R. multiflora, and S. babylonica. Nymphs reared on C. orbiculata weighed significantly less than those reared on V. labrusca or A. altissima; however, no significant difference was seen when their weights were compared with those reared on R. multiflora or S. babylonica. Nymphs reared on O. basilicum and A. rubrum weighed significantly less than nymphs reared on all other hosts.

Mean time (days) spent in instar and weight (mg) of Lycorma delicatula nymphs reared in 2021 by host plant species and instar. Triangles represent males, whereas squares represent females for second instar nymphs. Means with a different letter are significantly different from each other at a p-value < 0.05 using Tukey–Kramer grouping.

For second-instar nymphs, both host plant species (F 5,186 = 9.25; p < 0.0001) ( Figure 4 ) and the interaction of host plant species and sex (F 5,186 = 3.25; p = 0.008) had significant effects on the mean development time in the second instar. Sex alone did not have a significant effect (F 1,186 = 0.62; p = 0.432) on the mean development time in the second instar. When reared on A. rubrum, males spent significantly longer in that instar than females. In addition, male nymphs reared on A. rubrum took significantly longer than second-instar nymphs reared on any other host, except for R. multiflora. None of the nymphs reared on O. basilicum were able to complete the second instar.

For the second-instar nymphs, sex had a significant effect on their mean weight (F 1,186 = 113.27; p < 0.0001) ( Figure 4 ), with female nymphs weighing more than male nymphs. Host plant species also significantly impacted the mean weight of nymphs (F 1,186 = 22.25; p < 0.0001); however, the interaction of host plant species and sex was not significant (F 1,186 = 0.22; p = 0.956). Female nymphs reared on S. babylonica weighed significantly more than female nymphs reared on C. orbiculate; however, no significant difference was seen when their weights were compared with the weights of female nymphs reared on V. labrusca, A. altissima, and S. babylonica. Female nymphs reared on these hosts weighed significantly more than female nymphs reared on C. orbiculata, A. rubrum, or R. multiflora. No significant difference in weight was found between male nymphs reared on V. labrusca, C. orbiculata, A. altissima, and S. babylonica; however, male nymphs reared on either V. labrusca, A. altissima, or S. babylonica weighed more than male nymphs reared on either R. multiflora or A. rubrum.

 2020 adult mass and morphometrics

Host plant species (F 2,59 = 3.97; p = 0.024), sex (F 1,59 = 32.42; p < 0.0001), and the interaction of host plant species and sex (F 2,59 = 8.02; p = 0.001), had a significant effect on the mean weight of adults that completed the fourth instar in 2020 ( Table 2 ). Adult female nymphs that completed the fourth instar, and which had been reared on either A. altissima or V. labrusca, weighed significantly more than male adult nymphs that completed the fourth instar, and which had been reared on A. altissima, V. labrusca, or S. babylonica, as well as female adult nymphs that completed the fourth instar and had been reared on S. babylonica. Likewise, there was no significant difference in the mean weight of male and female adult nymphs that completed the fourth instar and which had been reared on either A. altissima or V. labrusca. There was also no significant difference observed in the mean weight of adult male nymphs that completed the fourth instar and were reared on A. altissima, V. labrusca, and S. babylonica, or female adults that completed the fourth instar and were reared on S. babylonica.

Mean [± SE (n)] adult Lycorma delicatula body weight (g), forewing length (mm) and width (mm), and hind tibia length (mm) at different combinations of host plant species and sex in L. delicatula reared on three host plants in 2020.

Measure Host plant species and sex a Statistics
Ailanthus altissima Vitis labrusca Salix babylonica
Male Female Male Female Male Female F d.f. p-value
Weight (g) 0.120 ± 0.003b (32) 0.175 ± 0.006a (14) 0.124 ± 0.004b (12) 0.187 ± 0.006a (20) 0.124 ± 0.01b (4) 0.115b (1) 8.02 2,59 0.001
Forewing length (mm) 17.43 ± 0.22b (31) 21.43 ± 0.39a (13) 18.01 ± 0.35b (12) 21.55 ± 0.33a (20) 17.65 ± 0.59b (4) 18.39ab (1) 2.42 2,68.73 0.097
Forewing width (mm) 7.683 ± 0.11b (31) 9.18 ± 0.19a (13) 7.852 ± 0.18b (12) 9.361 ± 0.11a (20) 7.585 ± 0.30b (4) 7.56ab (1) 2.01 2,75 0.142
Hind tibia length (mm) 9.875 ± 0.10b (31) 11.05 ± 0.16a (13) 10.08 ± 0.15b (12) 11.15 ± 0.14a (20) 9.79 ± 0.28b (4) 9.58ab (1) 2.84 2,69.31 0.066

Means followed by the same letter are not significantly different at a p-value ≤ 0.05 using Tukey–Kramer grouping. Sample size (N) is the number of survivors.

d.f., degrees of freedom.

Sex had a significant effect on adult forewing length (F 1,64.92 = 28.16; p < 0.0001), whereas host plant species (F 2,20.14 = 2.81; p = 0.084) and the interaction of host plant species and sex (F 2,68.73 = 2.42, p = 0.097) did not ( Table 2 ). Both host plant species (F 2,75 = 3.71; p = 0.029) and sex (F 1,75 = 13.32; p = 0.001) had a significant effect on the forewing width of adult nymphs; however, the interaction of host plant species and sex (F 2,75 = 2.01; p = 0.142) did not. Female adult nymphs reared on either A. altissima or V. labrusca had significantly wider and longer forewings than male nymphs reared on these hosts. Likewise, host plant species (F 2,19.75 = 3.93; p = 0.037) and sex (F 1,64.45 = 10.49; p = 0.002) had a significant effect on adult hind tibia length, whereas the interaction of host plant species and sex (F 2 69.31 = 2.84; p = 0.066) had no significant effect. The hind tibia length of female adult nymphs that completed development on either A. altissima or V. labrusca was significantly longer than the hind tibia length of adult male nymphs reared on these two hosts.

 Discussion

Host plant species had an effect on nymphal survival. Nymphs reared on A. altissima and V. labrusca survived equally well, but survival was decreased for those reared on R. multiflora, A. rubrum, and O. basilicum. Nymphs failed to develop through the second instar on O. basilicum and through the third and fourth instars on A. rubrum. Host plant species was found to have a significant effect on the development time of L. delicatula nymphs in the first through third instars. Host plant species was also found to have a significant effect on the mean weight of nymphs in the first, second, and fourth, but not in the third, instars. Host plant species had a significant effect on adult hind tibia length and forewing width. These findings should be incorporated into phenology models for L. delicatula to account for host effects.

First-instar nymphs reared on A. rubrum and O. basilicum took longer to develop and later, as second-instar nymphs, had the lowest weights. The inability of second-instar nymphs reared on O. basilicum to complete the second instar, and of third- and fourth-instar nymphs reared on A. rubrum to complete development, suggests that the performance of earlier instars is indicative of host viability for later instars. Declining host viability as nymphal development progresses was also seen in the percentage survival of second- through fourth-instar nymphs in 2020; specifically, second-instar nymphs reared on S. babylonica had a similar percentage survival to those reared on either A. altissima or V. labrusca. This trend of reduced viability as development progresses can also be seen in previous research, in which a shift away from R. multiflora as the dominant host was observed in the L. delicatula third instar (13). In addition, this trend of reduced survival on hosts where nymphs take longer to develop in earlier instars is also seen in H. halys nymphs (8). Another study found that the number of host plants on which L. delicatula nymphs could complete the first instar was higher than the number on which it could complete the second instar (14). In addition, in that study, second-instar nymphs reared on A. rubrum failed to complete that instar, and third-instar nymphs reared on S. babylonica failed to complete that instar, supporting the results seen for those hosts with later-instar nymphs in this study. Interestingly, L. delicatula has consistently good survival during all instars on its preferred host, A. altissima. Furthermore, life stages where host plant species had a significant effect on the mean time spent in each instar, A. altissima was one of the hosts that nymphs spent the least amount of time feeding on during each instar, as inferred from the slow-growth, high-mortality hypothesis (15). In many cases, there was no significant difference in mean development time in instar between nymphs reared on V. labrusca and those reared on A. altissima, thus suggesting that V. labrusca is comparable to A. altissima as a host for L. delicatula nymphs. The interaction of host and sex had a significant effect on the mean time spent in instar only for second-instar nymphs; however, that is most likely a result of the fact that male second-instar nymphs reared on A. rubrum spent significantly longer in that instar than nymphs reared on other hosts, excluding R. multiflora.

Host plant species also had a significant effect on the mean weight of L. delicatula nymphs in the first and second instars. This difference in weight is more likely explained by nutritional differences in the host plant, rather than by differences in plant defensive compounds, as L. delicatula is known to sequester defensive compounds (16). Mean weight was also significantly affected by sex in the second, third, and fourth instars. The significant effect on mean weight of the interaction of host and sex in fourth-instar nymphs may limit the use of weight for sexing L. delicatula nymphs. The differences between sexes in development time and weight were reflective of each other, as the lighter males took less time to develop than the heavier females. Lower weights in fourth-instar nymphs were also associated with less optimal temperatures in previous research, which further hints at S. babylonica being a less optimal host than either A. altissima or V. labrusca for female fourth-instar nymphs (9). The general similarities in the mean time spent in an instar for first- and second-instar nymphs among different host plant species suggest that weight might be a better indicator of host suitability for those instars. For first-instar nymphs, longer developmental times also resulted in nymphs with lower weights. Growth rate affects the size of an individual, but the final size is determined by factors that terminate growth and lead to a molt. Many insects have a critical weight they must achieve before they molt and, if this weight is not reached, they do not survive. The critical weight has been determined for Manduca sexta L. (Lepidoptera: Sphingidae), and molting frequency is associated with growth rate (17). In addition, slower growth rate has been seen in M. sexta in response to suboptimal temperatures or nutrition, which matches the trend shown in this study for L. delicatula. Thus, it may be the case that, on suboptimal hosts, reach the critical weights for each instar only just before molting.

Adult morphometrics differed by sex, further suggesting that there is size-based sexual dimorphism in L. delicatula adults. Host plant species had a significant effect on the hind tibia length and forewing width of L. delicatula adults. These factors are more indicative of nymph size than forewing length, which has been shown to affect the flight capabilities of L. delicatula (18). In L. delicatula adults, weight appears to be a proxy for sex and nourishment level. Nourishment level could have an impact on nymph flight capabilities, and this is particularly important in the context of dispersal, as extra nourishment could be used to sustain longer flights (19). Heavier weights can also allow adult to persist longer without food sources, as seen with other hemipterans, and thus increase the odds of individual nymphs surviving human-mediated dispersal events, such as those occurring on planes or cargo ships (20). Landscape-level decisions, in terms of host quality for L. delicatula, could also play a role in dispersal through shipping hubs, ports, and airfields.

The results of this study have implications for phenology models for L. delicatula because phenology is affected by the host plant that individual nymphs feed on. Dynamic models accounting for host preference by instar are needed moving forward, so that accurate predictions of phenology can be made. As the mean development time did not differ significantly between nymphs reared on either A. altissima or V. labrusca, the degree-day requirements from Kreitman et al. (9) should be viable for degree-day modeling for monitoring the growth of L. delicatula in vineyards, where the development of L. delicatula on grape plants takes 12.6–12.77 days to complete. Regardless of which host the second-instar nymphs were reared on, the time spent in that instar was shorter than the time spent in the second instar at 25°C in the previously mentioned study. However, as the previous study did not account for sex in that instar, it could potentially not be a true comparison. Furthermore, the use of plastic tubes in that study and the use of the BugDorm cages in this one makes it harder to make comparisons because the cages could hold more, and larger, host plants. This same trend was also observed for nearly every host plant in the case of third-instar nymphs, with the exception of female third-instar nymphs reared on S. babylonica. The same trend was apparent with fourth-instar nymphs, which in both studies accounted for their sex. This difference in developmental rates between the two studies only confirms the disadvantages of using plastic tubes over other containers for rearing nymphs. This is different from previous research that found that both first- and second-instar nymphs took longer to develop on Vitis rotundifolia var. Carlos (Michaux) than on A. altissima (21). This suggests that L. delicatula nymphs perform differently depending on the variant and species of Vitis that they are reared on. Further studies that look at different host plant species and use a combination of host plant species similar to that found in forest and landscape environments are needed to get a better idea of how different host plant species influence the development of L. delicatula nymphs.

Overall, this study shows that the development of L. delicatula can be influenced by host plant species. Moving forward, it is important to consider potential host options when developing management strategies for L. delicatula. Furthermore, this research can be extrapolated to identify what nutrients L. delicatula require to complete development based on their host utilization. The differences in development time by host plant species indicate a potential issue regarding the use of phenology models to predict the current life stage. Sampling for field data to use for validating phenology models is important and might be affected at a site level by the host plants that are present. As being reared on certain host plants results in nymphs having a lower weight, this study can also inform host plant choice when mass rearing L. delicatula for potential parasitoid use. The risk of L. delicatula damage to O. basilicum in homeowner gardens seems to be minimal, as O. basilicum does not appear to be a viable host for later instar nymphs. It is important for further research evaluating L. delicatula nymphal utilization of other species of Vitis to be undertaken, as its presence is a major threat to grape production.

 Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

 Author contributions

Part of DK’s M.Sc. thesis. Authors jointly conceived the study and got the funding for it. MK and DK collected and analyzed the data. MK prepared the figures. DK wrote the first draft of the paper, and all authors edited the manuscript. All authors contributed to the article and approved the submitted version.

 Funding

This work was funded in part by the USDA APHIS PPQ S&T interagency agreement 20–8130–0840-IA (FS 21IA11242303019) with the Forest Service and the AP19PPQS&T00C117 cooperative agreement with Rutgers University. The Northern Research Station, US Forest Service also partly funded this work.

 Acknowledgments

We thank L. Nixon and C. Rodriguez-Saona for critically reviewing the manuscript, and the reviewers for their input that improved the paper. We thank P. Moore, J. Richards, and N. Lowe for their technical assistance. We also thank K. Gundersen-Thorpe, P. Kallesser, L. Nixon, R. Rick, D. Supple, and T. Trotter for their help in obtaining permission to collect, or in collecting, the egg masses used in these studies.

 Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

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