Front. Immunol.Frontiers in ImmunologyFront. Immunol.1664-3224Frontiers Media S.A.10.3389/fimmu.2025.1645773ImmunologyCorrectionCorrection: HDAC6 inhibition by ITF3756 modulates PD-L1 expression and monocyte phenotype: insights for a promising immune checkpoint blockade co-treatment therapySpadottoValeria1†RipamontiChiara1†GhiroldiAndrea1GalbiatiElisabetta2PozziPietro2NoberiniRoberta3BonaldiTiziana34SteinkühlerChristian1FossatiGianluca1*1New Drug Incubator Department, Italfarmaco Group, Milan, Italy2Preclinical Drug Development Department, Italfarmaco Group, Milan, Italy3Department of Experimental Oncology, IEO European Institute of Oncology IRCCS, Milan, Italy4Department of Oncology and Hematology-Oncology (DIPO), University of Milan, Milan, Italy
Edited and Reviewed by: S. Paul Gao, Memorial Sloan Kettering Cancer Center, United States
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A Correction on HDAC6 inhibition by ITF3756 modulates PD-L1 expression and monocyte phenotype: insights for a promising immune checkpoint blockade co-treatment therapy By Spadotto V, Ripamonti C, Ghiroldi A, Galbiati E, Pozzi P, Noberini R, Bonaldi T, Steinkühler C and Fossati G (2025). Front. Immunol. 16:1546939. doi: 10.3389/fimmu.2025.1546939HDAC6monocytesimmuno-checkpointsTNF-αdendritic cellssection-in-acceptanceCancer Immunity and Immunotherapy
In the published article, there was an error in Figure 6 as published. There was a mistake in the axes of Figure 6C and Figure 6D as published, and as a result, the list of the genes were not correct. The corrected Figure 6 and its caption “ITF3756 downregulates monocytes activation and differentiation markers activated by TNF-α and promotes a less immunosuppressive phenotype in TNF-α stimulated monocytes. Purified human monocytes were treated for 2h with ITF3756 (1μM) and then stimulated with TNF-α (100ng/ml) for 4h. RNAseq data obtained as described before were used for this analysis. (A) Analysis of the modulation of specific markers of monocytes-derived cell population by TNF-α (left panel) and by the combination of TNF-α and ITF3756 (right panel). Fold changes (FC) are calculated versus the unstimulated control cells or versus the TNF-α stimulated cells, respectively. (B–D) Analysis of the modulation by TNF-α and by the combination of TNF-α and ITF3756 of a list of inhibitory immune checkpoints (31). Fold changes (FC) are calculated versus the unstimulated control cells in (B), versus the TNF-α stimulated cells in (C) and between ITF3756 and unstimulated control cells in (D). Significant differentially expressed genes are represented as circles, while non-significant genes are shown as triangles” appear below.
ITF3756 downregulates monocytes activation and differentiation markers activated by TNF-α and promotes a less immunosuppressive phenotype in TNF-α stimulated monocytes. Purified human monocytes were treated for 2h with ITF3756 (1μM) and then stimulated with TNF-α (100ng/ml) for 4h. RNAseq data obtained as described before were used for this analysis. (A) Analysis of the modulation of specific markers of monocytes-derived cell population by TNF-α (left panel) and by the combination of TNF-α and ITF3756 (right panel). Fold changes (FC) are calculated versus the unstimulated control cells or versus the TNF-α stimulated cells, respectively. (B–D) Analysis of the modulation by TNF-α and by the combination of TNF-α and ITF3756 of a list of inhibitory immune checkpoints (31). Fold changes (FC) are calculated versus the unstimulated control cells in (B), versus the TNF-α stimulated cells in (C) and between ITF3756 and unstimulated control cells in (D). Significant differentially expressed genes are represented as circles, while non-significant genes are shown as triangles.
Four scatter plots (A, B, C, D) display log2 fold changes for gene expression under different conditions. Plots show significance and markers of various cell types, with dot sizes indicating base mean levels. Graph A includes diverse cell markers like monocytes and dendritic cells, with colors distinguishing them. Graphs B, C, and D are green, indicating different settings and focus on inhibitory immune checkpoints. Triangles and circles represent significance, with annotations provided for each shape.
The original article has been updated.
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