Front. Immunol. Frontiers in Immunology Front. Immunol. 1664-3224 Frontiers Media S.A. 10.3389/fimmu.2025.1645241 Immunology Correction Correction: Endogenous SLPI contributes to the regulation of inflammatory responses in peritoneal macrophages by modulating MMP-9 production Tyshchenko Mariia 1 2 Pocałuń Natalia 1 2 Kwiecińska Patrycja 1 † Cichy Joanna 1 Wilk Mieszko M. 1 Oleszycka Ewa 1 * 1 Department of Immunology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Kraków, Poland 2 Doctoral School of Exact and Natural Sciences, Jagiellonian University, Kraków, Poland

Approved by: Frontiers Editorial Office, Frontiers Media SA, Switzerland

*Correspondence: Ewa Oleszycka, ewa.oleszycka@uj.edu.pl

†Present address: Patrycja Kwiecińska, Laboratory of Stem Cell Biology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Kraków, Poland

 23 07 2025 2025 16 1645241 11 06 2025 12 06 2025 Copyright © 2025 Tyshchenko, Pocałuń, Kwiecińska, Cichy, Wilk and Oleszycka 2025 Tyshchenko, Pocałuń, Kwiecińska, Cichy, Wilk and Oleszycka

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 A Correction on Endogenous SLPI contributes to the regulation of inflammatory responses in peritoneal macrophages by modulating MMP-9 production By Tyshchenko M, Pocałuń N, Kwiecińska P, Cichy J, Wilk MM and Oleszycka E (2025) Front. Immunol. 16:1563845. doi: 10.3389/fimmu.2025.1563845 SLPI proteinase inhibitor LPS septic shock inflammation monocytes peritoneal macrophages MMP-9 (matrix metalloproteinase-9) 2020/39/D/NZ6/00798 Narodowe Centrum Nauki10.13039/501100004281 section-in-acceptance Cytokines and Soluble Mediators in Immunity

There was a mistake in Figure 3 as published. The final version of Figure 3 which included additional panel of zymography ( Figure 3G ) was not included during post-acceptance proofs and pre-peer review version of Figure 3 was published by mistake. The correct version of Figure 3 appears below.

SLPI regulates MMP-9 secretion in resident peritoneal macrophages. (A) SLPI, IL-6 and TNF in supernatants of WT and Slpi-/- resident peritoneal macrophages incubated with LPS (100 ng/ml) for 24h.WT control vs WT LPS ** p< 0.01 by one-way ANOVA, Tuckey post hoc test. (B) IL-1α and IL-1β in supernatants of WT and Slpi-/- peritoneal macrophages incubated with LPS (100 ng/ml) for 3 h followed by addition of alum (100 µg/ml) or nigericin (10 µM). (C) Total cell and neutrophil count in WT and Slpi-/- mice injected i.p. with PBS or 100 μg alum for 24h. (D) Representative immunoblots of MMP-9 and SLPI in supernatants and lysates of WT and Slpi-/- peritoneal macrophages incubated with LPS (100 ng/ml) for 24h.Samples were resolved by SDS-PAGE and probed by Western blotting. β-actin was used as loading control. (E) Densitometry analysis of (D) WT LPS vs Slpi-/- LPS ***p < 0.001 by one-way ANOVA, Tukey post hoc test. (F) MMP-9 in supernatants of WT and Slpi-/- resident peritoneal macrophages incubated with LPS (100 ng/ml) for indicated times. WT LPS vs Slpi-/- LPS ***p < 0.001 by one-way ANOVA, Tukey post hoc test. (G) Representative zymography of equal volume of supernatants obtained from WT and Slpi-/- peritoneal macrophages incubated with LPS (100 ng/ml) for 24h.Representative of four separate experiments. (H) MMP-9 in supernatants of WT and Slpi-/- resident peritoneal macrophages incubated with DMSO or inhibitors: JNK-INH-8 (10 μM), SP600125 (20 μM), PD98059 (20 μM) or SB203580 (10 μM) for 1h followed by LPS treatment (100 ng/ml) for 24h.LPS vs LPS + inhibitor *p<0.05 by one-way ANOVA, Tukey post hoc test. (I) SLPI, IL-6 and MMP-9 in supernatants of WT and Slpi-/- resident peritoneal macrophages incubated with LPS (100 ng/ml) and IFN-γ (50 ng/ml) for 24h.Control vs IFN−γ *p<0.05, ***p<0.001 by multiple unpaired t-test. (A, B, F, H, I) Data are presented as the mean of three (A, B, F) or four (H, I) independent experiments. Error bars show means ± SEM. (C) Data represent 5 to 6 mice per experimental group pooled from two independent experiments. Error bars show means ± SEM.

Graphs and images present scientific data on the effects of control, alum, and LPS treatments on various markers like SLPI, IL-6, IL-1α, IL-1β, TNF, and MMP-9 in wild-type (WT) and Slpi-/- cells. Key findings include differential expression levels in cell lysates and supernatants, showcased through bar graphs and Western blot images. Significance is marked by asterisks, and some values are labeled as non-detectable (nd). Data highlights the role of SLPI in inflammatory responses.