AUTHOR=Nazki Salik , Reddy Vishwanatha R. A. P. , Kamble Nitin , Sadeyen Jean-Remy , Iqbal Munir , Behboudi Shahriar , Shelton Holly , Broadbent Andrew J. 

TITLE=CD4+TGFβ+ cells infiltrated the bursa of Fabricius following IBDV infection, and correlated with a delayed viral clearance, but did not correlate with disease severity, or immunosuppression

JOURNAL=Frontiers in Immunology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1197746

DOI=10.3389/fimmu.2023.1197746

ISSN=1664-3224

ABSTRACT=Infectious Bursal Disease Virus (IBDV) causes immunosuppression in chickens. While B-cell destruction is the main cause of humoral immunosuppression, bursal T cells from IBDV-infected birds have been reported to inhibit the mitogenic response of splenocytes, indicating that some T cell subsets in the infected bursa have immunomodulatory activities. CD4 + CD25 + TGFβ + cells have been recently described in chickens that have immunoregulatory properties and play a role in the pathogenesis of Marek's Disease Virus. To evaluate if they infiltrated the bursa of Fabricius (BF) following IBDV infection, and influenced the outcome of infection, birds were inoculated at either 2 days or 2 weeks of age with vaccine strain (228E), classic field strain (F52-70), or PBS (mock). Both 228E and F52-70 led to atrophy of the BF, a significant reduction of Bu1 + -B cells, and a significant increase in CD4 + and CD8α + T cells in the BF, but only F52/70 caused suppression of immune responses to a test antigen in younger birds, and clinical signs in older birds. Virus was cleared from the BF more rapidly in younger birds than older birds. An infiltration of CD4 + CD25 + T cells into the BF, and elevated expression of bursal TGFβ-1 + mRNA was observed at all time points following infection, irrespective of the strain or age of the birds, but CD4 + TGFβ + cells and CD4 + CD25 + TGFβ + cells only appeared in the BF at 28dpi in younger birds. In older birds, CD4 + TGFβ + cells and CD4 + CD25 + TGFβ + cells were present at earlier time points, from 7dpi following 228E infection, and from 14 and 28dpi following F52/70 infection, respectively. Taken together, our data suggest that an earlier infiltration of CD4 + TGFβ + cells into the BF correlated with a delayed clearance of virus. However, the influx of CD4 + TGFβ + cells and CD4 + CD25 + TGFβ + into the BF did not correlate with increased pathogenicity, or immunosuppression.