AUTHOR=Chen Lei , Lu Jiansheng , Yue Junjie , Wang Rong , Du Peng , Yu Yunzhou , Guo Jiazheng , Wang Xi , Jiang Yujia , Cheng Kexuan , Yang Zhixin , Zheng Tao 

TITLE=A humanized anti-human adenovirus 55 monoclonal antibody with good neutralization ability

JOURNAL=Frontiers in Immunology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1132822

DOI=10.3389/fimmu.2023.1132822

ISSN=1664-3224

ABSTRACT=Human adenovirus type 55 (HAdV55) has a re-emerged as pathogen causing an acute respiratory disease presenting as a severe lower respiratory illness that can cause death. To date, there is no HAdV55 vaccine or treatment available for general use. Herein, a monoclonal antibody specific for HAdV55, mAb 9-8, was isolated from an scFv-phage display library derived from mice immunized with the purified inactived-HAdV55 virions. mAb 9-8 showed potent neutralization activity against HAdV55. After humanization, the humanized neutralizing monoclonal antibody (9-8-h2) was identified to neutralize HAdV55 infection with an IC50 of 0.3886 nM. The mAb 9-8-h2 recognized HAdV55 and HAdV7 virus particles, but not HAdV4 particles. Although mAb 9-8-h2 could recognize HAdV7, it could not neutralize HAdV7. Furthermore, mAb 9-8-h2 recognized a conformational neutralization epitope of the fiber protein and the crucial amino acid residues (Arg 288, Asp 157, and Asn 200) were identified. MAb 9-8-h2 also showed favorable general physicochemical properties, including good thermostability and pH stability. Overall, mAb 9-8-h2 might be a promising molecule for the prevention and treatment of HAdV55.