AUTHOR=Xiao Chunying , Zhu Zhenlai , Zhang Chen , Gao Jixin , Luo Yixin , Fang Hui , Qiao Hongjiang , Li Wei , Wang Gang , Fu Meng 
  
TITLE=A population of dermal Langerin+ dendritic cells promote the inflammation in mouse model of atopic dermatitis
  
JOURNAL=Frontiers in Immunology
  
VOLUME=Volume 13 - 2022
  
YEAR=2022
  
URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.981819
  
DOI=10.3389/fimmu.2022.981819
  
ISSN=1664-3224
  
ABSTRACT=Cutaneous dendritic cells (DCs) have been implicated in the pathogenesis of atopic dermatitis (AD); however, the role of specific subset of DCs has not been well defined. Here, we investigated the contributions of Langerhans cells (LCs), resident dermal Langerin+ DCs (r-Langerin+ dDCs) and newly infiltrated inflammatory dermal Langerin+ DCs (i-Langerin+ dDCs) in AD mouse model induced by topical application of MC903. We found that depletion of i-Langerin+ dDCs only in DTR mice after multiple DT injection resulted in significant reduction of thymic stromal lymphopoietin (TSLP) production and inflammation alleviation, while depletion of LCs or r-Langerin+ dDCs in DTA mice or DTR mice with single DT injection resulted in no significant inflammation changes compared to the wild-type (WT) mice. DT-treated DTR-WT mice with depletion of BM-derived i-Langerin+ dDCs resulted in markedly lower inflammation than that of controls, while PBS-treated chimeric mice (DTR-WT) with only depletion of r-Langerin+ dDCs had a comparable inflammation to WT mice. Furthermore, we found that TSLP contributed to the upregulation of Langerin expression in bone marrow derived DCs and promoted the maturation of Langerin+ DCs. The current study demonstrated that the newly infiltrated inflammatory dermal Langerin+ DCs were essential for TSLP production, which conversely promoted the production of BM-derived i-Langerin+ dDCs, and participated in the inflammation of AD.