Front. Immunol. Frontiers in Immunology Front. Immunol. 1664-3224 Frontiers Media S.A. 10.3389/fimmu.2022.932151 Immunology Correction Corrigendum: Escape of TLR5 recognition by Leptospira spp.: A rationale for atypical endoflagella HolzapfelMarion 1 2 3 † BonhommeDelphine 1 2 3 4 † CaglieroJulie 5 Vernel-PauillacFrédérique 1 2 3 d’AndonMartine Fanton 1 2 3 BortolussiSophia 1 2 3 FietteLaurence 6 ‡ GoarantCyrille 7 WunderElsio A. Jr. 8 9 PicardeauMathieu 10 KoAlbert I. 8 9 WerlingDirk 11 MatsuiMariko 5 BonecaIvo G. 1 2 3 WertsCatherine 1 2 3 * 1Institut Pasteur, Unité Biologie et Génétique de la Paroi Bactérienne, Paris, France 2CNRS, UMR 2001 Microbiologie Intégrative et Moléculaire, Paris, France 3Institut National de la Santé et de la Recherche Médicale, Equipe Avenir, Paris, France 4Sorbonne Paris Cité, Université de Paris, Paris, France 5Institut Pasteur de Nouvelle Calédonie, Immunity and Inflammation Group, Institut Pasteur International Network, Noumea, France 6Unité Histopathologie Humaine et Modèles Animaux, Institut Pasteur, Paris, France 7Leptospirosis Research and Expertise Unit, Institut Pasteur International Network, Institut Pasteur de Nouvelle Calédonie, Noumea, France 8Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Brazil 9Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, United States 10Unité Biologie des Spirochètes, Institut Pasteur, Paris, France 11Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United Kingdom

Edited and Reviewed by: Melissa Jo Caimano, University of Connecticut Health Center, United States

*Correspondence: Catherine Werts, cwerts@pasteur.fr

This article was submitted to Microbial Immunology, a section of the journal Frontiers in Immunology

†These authors share first authorship

‡Present address: Laurence Fiette, IMMR, Paris, France

 08 11 2022 2022 13 932151 29 04 2022 25 05 2022 Copyright © 2022 Holzapfel, Bonhomme, Cagliero, Vernel-Pauillac, d’Andon, Bortolussi, Fiette, Goarant, Wunder, Picardeau, Ko, Werling, Matsui, Boneca and Werts 2022 Holzapfel, Bonhomme, Cagliero, Vernel-Pauillac, d’Andon, Bortolussi, Fiette, Goarant, Wunder, Picardeau, Ko, Werling, Matsui, Boneca and Werts

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 A Corrigendum on Escape of TLR5 Recognition by Leptospira spp.: A Rationale for Atypical Endoflagella By Holzapfel M, Bonhomme D, Cagliero J, Vernel-Pauillac F, Fanton d’Andon M, Bortolussi S, Fiette L, Goarant C, Wunder Jr EA, Picardeau M, Ko AI, Werling D, Matsui M, Boneca IG and Werts C (2020) Front. Immunol. 11:2007. doi: 10.3389/fimmu.2020.02007 Leptospira toll-like receptor innate immunity Flagelin genes TLR5 mouse model

In the original article, there was a mistake in Figure 8A, as published. We showed that we did not get expression of the FLaB1 subunit in Manilae L495 strain. In fact, the forward primer (designed according to the Fiocruz sequence) used to amplify the FlaB1 subunit has two mismatches within the Manilae sequence. We did the RT-PCR with the good primer and found an amplification, showing an enhanced expression at the stationary phase compared to the exponential phase, likewise the other FlaB subunits. The corrected Figure 8 appears below.

A correction has been made to Results, “FlaB mRNA Are Upregulated in Stationary Phase”.

“Of note, and different from other strains, the Manilae L495 flaB1 mRNA was undetectable” and a paragraph in the Discussion, “Furthermore, in Manilae L495…” are not accurate and should be both omitted.

In the original article, there was also mistake in Figure 6, Supplementary Figure 3, Supplementary Figure 4 and Supplementary Figure 6 and their legends as published. We realized a “slipping” of the names of FlaB subunits only in the figures of alignments and comparison of sequences (Figure 6, Sup Figure 3, Sup Figure 4 and Sup Figure 6). What we called FlaB1 in figures and respective legends is in fact FlaB4, FlaB2 is FlaB1, FlaB3 is FlaB2, and FlaB4 is FlaB3 (see corrected annotation in Additional table provided).

(A) Amino acid sequence homology average percentage between Salmonella typhimurium FliC (P06179) and Leptospira interrogans strain Fiocruz FlaB (LIC11890, LIC11889, LIC 11532 and LIC11531) and FlaAs (LIC10788 and LIC10787) and primary structures of the flagellin proteins with TLR5 binding consensus. (B) In silico (Phyre2 and Chimera softwares) prediction of Salmonella typhimurium FliC (P06179) structure with the four described domains and with positions of the TLR5 binding consensus: 1 (red), 2 (yellow) and 3 (light blue) and stabilization region (light green) highlighted. (C) In silico (Phyre2 and Chimera softwares) prediction of Leptospira interrogans strain Fiocruz FlaB4 (LICI1531) with the positions of the TLRS binding consensus and stabilization region highlighted, FlaA1 (LIC10788), FlaA2 (LICI0787). (D) Clustal (MEGA software) alignment of the amino acid sequences for the TLR5 binding consensus regions of: Salmonella enterica FliC (GeneBank QDQ31983.1), L. bifleva (strain Patoc) FlaB1 (LEPBla2133), FlaB2 (LEPBIa2132), FlaB3 (LEPBla1872) and FlaB4 (LEPBla1589), L. interrogans (strain Fiocruz L1-130) FlaB1 (LIC18890), FlaB2 (LICIT889), FlaB3 (LICI1532) and FlaB4 (LIC11531), L. interrogans (strain L495) FlaB1 (LMANv2 260016), FlaB2 (LMANv2 260015). FlaB3 (LMANv2 590024) and FlaB4 (LMANv2 590023), and L. interrogans (strain Verdun) FlaB1 (AKWP_v1_110429), FlaB2 (AKWP_v1_110428) and FlaB3 (AKWP_v1_110068) and FlaB4 (AKWP_v1_110067).

Additional Table Old annotations (published Frontiers Immunol 2020).

10788 Patoc Fiocruz L1-130 Manilae Verdun
flaA1 LEPBIa2335 LIC10788 LMANv2_260046 AKWP_v1_210009
flaA2 LEPBIa2336 LIC10787 LMANv2_260045 AKWP_v1_210008
flaB1 LEPBIa1589 LIC11531 LMANv2_590023 AKWP_v1_110067
flaB2 LEPBIa2133 LIC11890 LMANv2_260016 AKWP_v1_110429
flaB3 LEPBIa2132 LIC11889 LMANv2_260015 AKWP_v1_110428
flaB4 LEPBIa1872 LIC11532 LMANv2_590024 AKWP_v1_110068

New annotations consistent with studies from M Picardeau.

10788 Patoc* Fiocruz L1-130* Manilae* Verdun*
flaA1 LEPBIa2335 LIC10788 LMANv2_260046 / LIMLP_13775 AKWP_v1_210009
flaA2 LEPBIa2336 LIC10787 LMANv2_260045/ LIMLP_13780 AKWP_v1_210008
flaB1 LEPBIa2133 LIC11890 LMANv2_260016/ LIMLP_09410 AKWP_v1_110429
flaB2 LEPBIa2132 LIC11889 LMANv2_260015/LIMLP_09405 AKWP_v1_110428
flaB3 LEPBIa1872 LIC11532 LMANv2_590024/ LIMLP_07480 AKWP_v1_110068
flaB4 LEPBIa1589 LIC11531 LMANv2_590023/ LIMLP_07475 AKWP_v1_110067

*Annotation of L. biflexa serovar Patoc strain Patoc 1 (Patoc), L. interrogans serovar Copenhageni strain Fiocruz L1-130 (Fiocruz L1-130), L. interrogans serovar Manilae strain L495 (Manilae), L. interrogans serovar Manilae UP-MMC-NIID LP (Manilae) (ref Satou et al. 2015 ; PMID: 26272567).

L. interrogans serovar Icterohaemorrhagiae Verdun LP (Verdun) in https://mage.genoscope.cns.fr/microscope/home/index.php.

However, the annotations used in Figures 7, 8 were correct and all the mutants used are correct. The corrected Figure 6 and its corrected legend appears below. The corrected Supplementary Figures 3, 4, and 6 can be accessed from the original article.

(A) In vitro FlaBs mRNA expression in L. interrogans Copenhageni Fiocruz L1-130, Icterohaemorrhagiae Verdun and Manilae L495 at the exponential (E) and stationary (S) phase. Data of RT-qPCR are expressed as the relative mRNA quantities normalized to the expression of the lipl41 mRNA. Technical replicates are represented as dots and lines correspond to mean (+/- SD) of replicates (3 < n < 9). Statistically significant differences (Student t-test) are indicated. (B) In vivo FlaAs and (C) FlaBs mRNA expression in blood of infected mice (n=5, light blue) and hamsters (n-5, dark blue), 24 h post intraperitoneal infection with 2x108 virulent L. interrogans Icterohaemorrhagiae strain Verdun, compared with mRNA expression in culture in EMJH at 30° C. Data of RT-gPCR are expressed as the ratio of mRNA quantities relatives to the EMJH control. Individual animals are represented as dots and lines correspond to mean (+/- SD) of all animals. Statistically significant differences (Student t-test) are indicated with corresponding p values: * for p < 0.05; ** for p < 0.01 and *** for p < 0.001.

The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.

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