AUTHOR=Wang Xue , Qu Yunhe , Wang Yuan , Wang Xiang , Xu Jialei , Zhao Hailing , Zheng Donglin , Sun Lin , Tai Guihua , Zhou Yifa , Cheng Hairong 

TITLE=β-1,6-Glucan From Pleurotus eryngii Modulates the Immunity and Gut Microbiota

JOURNAL=Frontiers in Immunology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.859923

DOI=10.3389/fimmu.2022.859923

ISSN=1664-3224

ABSTRACT=Polysaccharides from Pleurotus eryngii have a variety of biological activities. In the present study, a homogeneous branched β-1,6-glucan named APEP-A-b was obtained from the fruiting bodies of P. eryngii and the effects on immunity and gut microbiota were investigated. Our results showed that APEP-A-b could significantly increase phagocytic capacity of peritoneal cavity phagocytes, splenic lymphocyte proliferation, and NK cell activity. Furthermore, the proportion of CD4+ T cells and CD8+ T cells in lamina propria were significantly increased upon APEP-A-b treatment. Additionally, APEP-A-b supplementation demonstrated pronounced differentiating microbiota, which indicated by a promotion of the relative abundances of Lachnospiraceae and Rikenellaceae family etc.. Consistently, APEP-A-b significantly increased the concentration of acetic acid and butyric acid in the cecum contents. Collectively, our results suggest that β-1,6-glucan from P. eryngii might enhance the immunity by modulating microbiota. These results are important for the structure-activity relationship of polysaccharides as well as processing and product development of P. eryngii derived polysaccharides.