AUTHOR=Wen Zhaohai , Aleem Muhammad Tahir , Aimulajiang Kalibixiati , Chen Cheng , Liang Meng , Song Xiaokai , Xu Lixin , Li Xiangrui , Yan Ruofeng 

TITLE=The GT1-TPS Structural Domain Protein From Haemonchus contortus Could Be Suppressive Antigen of Goat PBMCs

JOURNAL=Frontiers in Immunology

VOLUME=Volume 12 - 2021

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.787091

DOI=10.3389/fimmu.2021.787091

ISSN=1664-3224

ABSTRACT=Trehalose phosphate synthase (TPS) is one of the keys to the synthesis of trehalose, an enzyme that is not present in mammals but is critical to the viability of a wide range of lower organisms because trehalose is a key metabolite. However, almost nothing is known about the function of Hc-TPS (GT1-TPS structural domain protein from Haemonchus contortus). In the present study, the Hc-TPS gene was cloned and the recombinant protein (rHc-TPS) was expressed and purified. The qPCR results showed that Hc-TPS was transcribed at different developmental stages of Haemonchus contortus (H. contortus), with higher levels of transcription at the molt and embryo stages. Immunofluorescence analysis showed that Hc-TPS was widely distributed in adults, but the expression was mainly localized on the mucosal surface of the intestine and in the embryos of female worms. The impacts of rHc-TPS on PBMCs cell proliferation, nitric oxide (NO) generation, transcriptional expression of cytokines and related pathway molecules were examined by co-incubating rHc-TPS with goat PBMCs. The results showed that rHc-TPS significantly inhibited PBMCs proliferation and NO secretion in a dose-dependent manner. We also found that rHc-TPS activated the IL-10/STAT3/SOCS3 axis and significantly promoted SOCS3 expression, while inhibiting INF-γ, IL-4, IL-9 and IL-2 pathways. Our findings contribute to understand the procedure elaborate for protection against parasites during host-parasite interactions and also produce new ideas for the progress of new drug targets.